Chy2G044830 (gene) Cucumber (hystrix) v1

BLAST of Chy2G044830 vs. ExPASy Swiss-Prot
Match: P31684 (4-coumarate--CoA ligase 1 OS=Solanum tuberosum OX=4113 GN=4CL1 PE=3 SV=1)

HSP 1 Score: 745.3 bits (1923), Expect = 5.2e-214
Identity = 381/549 (69.40%), Postives = 450/549 (81.97%), Query Frame = 0

Query: 55  PMAAADVSSPEFIFRSKLPEIPISTHLPLHTYCFENISEFKRRPCLINAATGHTYTYGEV 114
           PM      S + IFRSKLP+I I  HLPLH+YCFEN+SEF  RPCLI+ A    YTY EV
Sbjct: 2   PMDTETKQSGDLIFRSKLPDIYIPKHLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEV 61

Query: 115 QATSRRVAAGLHKLGIGKGDVVMLLLQNTPEFVLAFLGASYLGAAATMANPFFMPSEIAK 174
           + TSR+VA GL+KLGI + D +M+LL N PEFV AF+GASYLGA +TMANP F P+E+ K
Sbjct: 62  ELTSRKVAVGLNKLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVK 121

Query: 175 QAVSSGAKVIITQAAFAEKVKNISQENEMIIKVIFIDD--DDPPQFSSLIEDVSKEEELG 234
           QA +S AK++ITQA FA KVK+ + EN+  +KVI +D   +    FS LI+  S E E+ 
Sbjct: 122 QAKASSAKIVITQACFAGKVKDYAIEND--LKVICVDSVPEGCVHFSELIQ--SDEHEI- 181

Query: 235 MGDVKISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLHIRSDDVVLCL 294
             DVKI PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGEN +L++ SDDV++C+
Sbjct: 182 -PDVKIQPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSDDVLMCV 241

Query: 295 LPLFHIYSLNSIMMCALRVGAAILIVQKFDVNSLVELVSKYKVTFAPFVPPIVLAIAKSP 354
           LPLFHIYSLNS+++CALRVGAAILI+QKFD+   +EL+ K+KVT  PFVPPIVLAIAKSP
Sbjct: 242 LPLFHIYSLNSVLLCALRVGAAILIMQKFDIAQFLELIPKHKVTIGPFVPPIVLAIAKSP 301

Query: 355 AVDHFDMSSLRIVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFVKEG 414
            VD++D+SS+R V+SGAAPLGK LEDA RAK P+  LGQGYGMTEAG V+ M LAF KE 
Sbjct: 302 LVDNYDLSSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEP 361

Query: 415 FGIKSGGCGTIMRNSEMKILNLQTGASLPRNQTGEICIRSSQMMKGYLNDEDATKAIIDE 474
           F IKSG CGT++RN+EMKI++  TG SLPRNQ GEICIR  Q+MKGYLND +AT   I++
Sbjct: 362 FDIKSGACGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATARTIEK 421

Query: 475 DGWLHTGDIGFVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHAHIADAAVIPMND 534
           +GWLHTGDIGF+DDDDELFIVDRLKELIKYKGFQVAPAELEALLI+H  I+DAAV+PM D
Sbjct: 422 EGWLHTGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVPMID 481

Query: 535 EVAGEVPVAFIVRFDGSNITEDEIKQFISKQVVFYKRINRVFFVDSIPKSPSGKILRRQL 594
           E AGEVPVAF+VR +GS ITEDE+K FISKQV+FYKRI RVFFV+++PKSPSGKILR+ L
Sbjct: 482 EQAGEVPVAFVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVETVPKSPSGKILRKDL 541

Query: 595 RALLAASIS 602
           RA LAA IS
Sbjct: 542 RARLAAGIS 544

BLAST of Chy2G044830 vs. ExPASy Swiss-Prot
Match: P31685 (4-coumarate--CoA ligase 2 OS=Solanum tuberosum OX=4113 GN=4CL2 PE=3 SV=1)

HSP 1 Score: 742.3 bits (1915), Expect = 4.4e-213
Identity = 380/549 (69.22%), Postives = 449/549 (81.79%), Query Frame = 0

Query: 55  PMAAADVSSPEFIFRSKLPEIPISTHLPLHTYCFENISEFKRRPCLINAATGHTYTYGEV 114
           PM      S + IFRSKLP+I I  HLPLH+YCFEN+SEF  RPCLI+ A    YTY EV
Sbjct: 2   PMDIETKQSGDLIFRSKLPDIYIPKHLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEV 61

Query: 115 QATSRRVAAGLHKLGIGKGDVVMLLLQNTPEFVLAFLGASYLGAAATMANPFFMPSEIAK 174
           + TSR+VA GL+KLGI + D +M+LL N PEFV AF+GASYLGA +TMANP F P+E+ K
Sbjct: 62  ELTSRKVAVGLNKLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVK 121

Query: 175 QAVSSGAKVIITQAAFAEKVKNISQENEMIIKVIFIDD--DDPPQFSSLIEDVSKEEELG 234
           QA +S AK++ITQA FA KVK+ + EN+  +KVI +D   +    FS LI+  S E E+ 
Sbjct: 122 QAKASSAKIVITQACFAGKVKDYAIEND--LKVICVDSAPEGCVHFSELIQ--SDEHEI- 181

Query: 235 MGDVKISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLHIRSDDVVLCL 294
             DVKI PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGEN +L++ SDDV++C+
Sbjct: 182 -PDVKIQPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSDDVLMCV 241

Query: 295 LPLFHIYSLNSIMMCALRVGAAILIVQKFDVNSLVELVSKYKVTFAPFVPPIVLAIAKSP 354
           LPLFHIYSLNS+++CALRVGAAILI+QKFD+   +EL+ K+KVT  PFVPPIVLAIAKSP
Sbjct: 242 LPLFHIYSLNSVLLCALRVGAAILIMQKFDIAQFLELIPKHKVTIGPFVPPIVLAIAKSP 301

Query: 355 AVDHFDMSSLRIVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFVKEG 414
            V ++D+SS+R V+SGAAPLGK LEDA RAK P+  LGQGYGMTEAG V+ M LAF KE 
Sbjct: 302 LVHNYDLSSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEP 361

Query: 415 FGIKSGGCGTIMRNSEMKILNLQTGASLPRNQTGEICIRSSQMMKGYLNDEDATKAIIDE 474
           F IKSG CGT++RN+EMKI++  TG SLPRNQ GEICIR  Q+MKGYLND +AT   I++
Sbjct: 362 FDIKSGACGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATARTIEK 421

Query: 475 DGWLHTGDIGFVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHAHIADAAVIPMND 534
           +GWLHTGDIGF+DDDDELFIVDRLKELIKYKGFQVAPAELEALLI+H  I+DAAV+PM D
Sbjct: 422 EGWLHTGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVPMID 481

Query: 535 EVAGEVPVAFIVRFDGSNITEDEIKQFISKQVVFYKRINRVFFVDSIPKSPSGKILRRQL 594
           E AGEVPVAF+VR +GS ITEDE+K FISKQV+FYKRI RVFFV+++PKSPSGKILR+ L
Sbjct: 482 EQAGEVPVAFVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVETVPKSPSGKILRKDL 541

Query: 595 RALLAASIS 602
           RA LAA IS
Sbjct: 542 RARLAAGIS 544

BLAST of Chy2G044830 vs. ExPASy Swiss-Prot
Match: O24146 (4-coumarate--CoA ligase 2 OS=Nicotiana tabacum OX=4097 GN=4CL2 PE=1 SV=1)

HSP 1 Score: 738.4 bits (1905), Expect = 6.4e-212
Identity = 370/541 (68.39%), Postives = 443/541 (81.89%), Query Frame = 0

Query: 60  DVSSPEFIFRSKLPEIPISTHLPLHTYCFENISEFKRRPCLINAATGHTYTYGEVQATSR 119
           D    + IFRSKLP+I I  HLPLH+YCFENISEF  RPCLIN A    YTY +V+  SR
Sbjct: 4   DTKQVDIIFRSKLPDIYIPNHLPLHSYCFENISEFSSRPCLINGANKQIYTYADVELNSR 63

Query: 120 RVAAGLHKLGIGKGDVVMLLLQNTPEFVLAFLGASYLGAAATMANPFFMPSEIAKQAVSS 179
           +VAAGLHK GI   D +M+LL N+PEFV AF+GASYLGA +TMANP F P+E+ KQA +S
Sbjct: 64  KVAAGLHKQGIQPKDTIMILLPNSPEFVFAFIGASYLGAISTMANPLFTPAEVVKQAKAS 123

Query: 180 GAKVIITQAAFAEKVKNISQENEMIIKVIFIDDDDPPQFSSLIEDVSKEEELGMGDVKIS 239
            AK+I+TQA    KVK+ + EN+  +K+I I  D  P+       +++  E  + +V+I 
Sbjct: 124 SAKIIVTQACHVNKVKDYAFEND--VKIICI--DSAPEGCLHFSVLTQANEHDIPEVEIQ 183

Query: 240 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLHIRSDDVVLCLLPLFHIY 299
           PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENP+L+I S+DV+LC+LPLFHIY
Sbjct: 184 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYIHSEDVMLCVLPLFHIY 243

Query: 300 SLNSIMMCALRVGAAILIVQKFDVNSLVELVSKYKVTFAPFVPPIVLAIAKSPAVDHFDM 359
           SLNS+++C LRVGAAILI+QKFD+ S +EL+ +YKVT  PFVPPIVLAIAKSP VD +D+
Sbjct: 244 SLNSVLLCGLRVGAAILIMQKFDIVSFLELIQRYKVTIGPFVPPIVLAIAKSPMVDDYDL 303

Query: 360 SSLRIVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFVKEGFGIKSGG 419
           SS+R V+SGAAPLGK LED  RAK P+  LGQGYGMTEAG V+ M LAF KE F IKSG 
Sbjct: 304 SSVRTVMSGAAPLGKELEDTVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFEIKSGA 363

Query: 420 CGTIMRNSEMKILNLQTGASLPRNQTGEICIRSSQMMKGYLNDEDATKAIIDEDGWLHTG 479
           CGT++RN+EMKI++ +TG SLPRNQ+GEICIR  Q+MKGYLND +AT   ID++GWL+TG
Sbjct: 364 CGTVVRNAEMKIVDPKTGNSLPRNQSGEICIRGDQIMKGYLNDPEATARTIDKEGWLYTG 423

Query: 480 DIGFVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHAHIADAAVIPMNDEVAGEVP 539
           DIG++DDDDELFIVDRLKELIKYKGFQVAPAELEALL++H +I+DAAV+PM DE AGEVP
Sbjct: 424 DIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMKDEQAGEVP 483

Query: 540 VAFIVRFDGSNITEDEIKQFISKQVVFYKRINRVFFVDSIPKSPSGKILRRQLRALLAAS 599
           VAF+VR +GS ITEDE+K FISKQV+FYKRI RVFFVD+IPKSPSGKILR+ LRA LAA 
Sbjct: 484 VAFVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVDAIPKSPSGKILRKDLRAKLAAG 540

Query: 600 I 601
           +
Sbjct: 544 L 540

BLAST of Chy2G044830 vs. ExPASy Swiss-Prot
Match: I3PB37 (4-coumarate:CoA ligase 1 OS=Petunia hybrida OX=4102 GN=4CL1 PE=1 SV=1)

HSP 1 Score: 736.5 bits (1900), Expect = 2.4e-211
Identity = 365/541 (67.47%), Postives = 443/541 (81.89%), Query Frame = 0

Query: 60  DVSSPEFIFRSKLPEIPISTHLPLHTYCFENISEFKRRPCLINAATGHTYTYGEVQATSR 119
           + +  + IFRSKLP+I I  HLPLH+YCFENISEF  RPCLIN A  H YTY +V+ TSR
Sbjct: 6   ETNQGDLIFRSKLPDIYIPKHLPLHSYCFENISEFSSRPCLINGANNHIYTYADVELTSR 65

Query: 120 RVAAGLHKLGIGKGDVVMLLLQNTPEFVLAFLGASYLGAAATMANPFFMPSEIAKQAVSS 179
           +VAAGL+KLGI + D +M+LL N+PEFV AF+GASYLGA +TMANP F P+E+ KQA +S
Sbjct: 66  KVAAGLNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVKQAKAS 125

Query: 180 GAKVIITQAAFAEKVKNISQENEMIIKVIFIDDDDPPQFSSLIEDVSKEEELGMGDVKIS 239
            AK+IITQA F  KVK+ + +N + +  I    D  P+      ++++ +E  + DVKI 
Sbjct: 126 NAKLIITQACFVNKVKDYAFDNNLNVICI----DSAPEGCIHFSELTQADEHDIPDVKIQ 185

Query: 240 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLHIRSDDVVLCLLPLFHIY 299
            DDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGEN +L++ S+DV++C+LPLFHIY
Sbjct: 186 SDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPLFHIY 245

Query: 300 SLNSIMMCALRVGAAILIVQKFDVNSLVELVSKYKVTFAPFVPPIVLAIAKSPAVDHFDM 359
           SLNS+++C LRVGAAILI+QKFD+    EL+ KYKVT  PFVPPIVLAIAKSP VD++D+
Sbjct: 246 SLNSVLLCGLRVGAAILIMQKFDIVQFCELIEKYKVTIGPFVPPIVLAIAKSPVVDNYDL 305

Query: 360 SSLRIVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFVKEGFGIKSGG 419
           SS+R V+SGAAPLGK LEDA R K P+  LGQGYGMTEAG V+ M LAF KE F IKSG 
Sbjct: 306 SSVRTVMSGAAPLGKELEDAVRIKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGA 365

Query: 420 CGTIMRNSEMKILNLQTGASLPRNQTGEICIRSSQMMKGYLNDEDATKAIIDEDGWLHTG 479
           CGT++RN+EMKI++  TG SLPRNQ GEICIR  Q+MKGYLND  AT   ID++GWLHTG
Sbjct: 366 CGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPAATTRTIDKEGWLHTG 425

Query: 480 DIGFVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHAHIADAAVIPMNDEVAGEVP 539
           DIG++D+DDELFIVDRLKELIKYKGFQVAPAELEALL++H +I+DAAV+PM DE AGEVP
Sbjct: 426 DIGYIDNDDELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMKDEQAGEVP 485

Query: 540 VAFIVRFDGSNITEDEIKQFISKQVVFYKRINRVFFVDSIPKSPSGKILRRQLRALLAAS 599
           VAF+VR +GS+ITEDE+K F+SKQV+FYKRI RVFFV+++PKSPSGKILR+ LRA LAA 
Sbjct: 486 VAFVVRSNGSDITEDEVKDFVSKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRARLAAG 542

Query: 600 I 601
           +
Sbjct: 546 V 542

BLAST of Chy2G044830 vs. ExPASy Swiss-Prot
Match: O24145 (4-coumarate--CoA ligase 1 OS=Nicotiana tabacum OX=4097 GN=4CL1 PE=2 SV=1)

HSP 1 Score: 736.5 bits (1900), Expect = 2.4e-211
Identity = 367/538 (68.22%), Postives = 443/538 (82.34%), Query Frame = 0

Query: 63  SPEFIFRSKLPEIPISTHLPLHTYCFENISEFKRRPCLINAATGHTYTYGEVQATSRRVA 122
           S + IFRSKLP+I I  HLPLH+YCFENISEF  RPCLIN A    YTY EV+ T R+VA
Sbjct: 12  SGDLIFRSKLPDIYIPKHLPLHSYCFENISEFSSRPCLINGANDQIYTYAEVELTCRKVA 71

Query: 123 AGLHKLGIGKGDVVMLLLQNTPEFVLAFLGASYLGAAATMANPFFMPSEIAKQAVSSGAK 182
            GL+KLGI + D +M+LL N+PEFV AF+GASYLGA +TMANP F P+E+ KQA +S AK
Sbjct: 72  VGLNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVKQAKASSAK 131

Query: 183 VIITQAAFAEKVKNISQENEMIIKVIFIDDDDPPQFSSLIEDVSKEEELGMGDVKISPDD 242
           +IITQ+ F  KVK+ + EN+  +KVI I  D  P+      ++++ +E  + +VKI PDD
Sbjct: 132 IIITQSCFVGKVKDYASEND--VKVICI--DSAPEGCLHFSELTQSDEHEIPEVKIQPDD 191

Query: 243 VVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLHIRSDDVVLCLLPLFHIYSLN 302
           VVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGEN +L++ S+DV++C+LPLFHIYSLN
Sbjct: 192 VVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPLFHIYSLN 251

Query: 303 SIMMCALRVGAAILIVQKFDVNSLVELVSKYKVTFAPFVPPIVLAIAKSPAVDHFDMSSL 362
           SI++C LRVGAAILI+QKFD+   +EL+ KYKV+  PFVPPIVLAIAKSP VD +D+SS+
Sbjct: 252 SILLCGLRVGAAILIMQKFDIAPFLELIQKYKVSIGPFVPPIVLAIAKSPIVDSYDLSSV 311

Query: 363 RIVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFVKEGFGIKSGGCGT 422
           R V+SGAAPLGK LEDA R K P+  LGQGYGMTEAG V+ M LAF KE F IKSG CGT
Sbjct: 312 RTVMSGAAPLGKELEDAVRTKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGACGT 371

Query: 423 IMRNSEMKILNLQTGASLPRNQTGEICIRSSQMMKGYLNDEDATKAIIDEDGWLHTGDIG 482
           ++RN+EMKI++  TG SLPRNQ GEICIR  Q+MKGYLND +AT   ID++GWLHTGDIG
Sbjct: 372 VVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATTRTIDKEGWLHTGDIG 431

Query: 483 FVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHAHIADAAVIPMNDEVAGEVPVAF 542
           F+D+DDELFIVDRLKELIKYKGFQVAPAE+EALL++H +I+DAAV+PM DE AGEVPVAF
Sbjct: 432 FIDEDDELFIVDRLKELIKYKGFQVAPAEIEALLLNHPNISDAAVVPMKDEQAGEVPVAF 491

Query: 543 IVRFDGSNITEDEIKQFISKQVVFYKRINRVFFVDSIPKSPSGKILRRQLRALLAASI 601
           +VR +GS ITEDE+K FISKQV+FYKR+ RVFFV+++PKSPSGKILR+ LRA LAA +
Sbjct: 492 VVRSNGSAITEDEVKDFISKQVIFYKRVKRVFFVETVPKSPSGKILRKDLRARLAAGV 545

BLAST of Chy2G044830 vs. ExPASy TrEMBL
Match: A0A0A0LWT6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G423020 PE=4 SV=1)

HSP 1 Score: 1038.9 bits (2685), Expect = 8.4e-300
Identity = 534/546 (97.80%), Postives = 540/546 (98.90%), Query Frame = 0

Query: 56  MAAADVSSPEFIFRSKLPEIPISTHLPLHTYCFENISEFKRRPCLINAATGHTYTYGEVQ 115
           MAAAD SSPEFIFRSKLPEIPISTHLPLHTYCFENISEFK RPCLINAATGH YTYGEVQ
Sbjct: 1   MAAADASSPEFIFRSKLPEIPISTHLPLHTYCFENISEFKHRPCLINAATGHIYTYGEVQ 60

Query: 116 ATSRRVAAGLHKLGIGKGDVVMLLLQNTPEFVLAFLGASYLGAAATMANPFFMPSEIAKQ 175
           ATSRRVAAGLHKLGIGKGDV+MLLLQNTPEFVLAFLGASYLGAAATMANPFFM SEIAKQ
Sbjct: 61  ATSRRVAAGLHKLGIGKGDVIMLLLQNTPEFVLAFLGASYLGAAATMANPFFMQSEIAKQ 120

Query: 176 AVSSGAKVIITQAAFAEKVKNISQENEMIIKVIFIDDDDPPQFSSLIEDVSKEEELGMGD 235
           AVSSGAKVIITQAAFAEKVKN+SQENEMIIKVIFIDD+DPPQFSSLIEDV+KEEEL MGD
Sbjct: 121 AVSSGAKVIITQAAFAEKVKNLSQENEMIIKVIFIDDNDPPQFSSLIEDVAKEEELEMGD 180

Query: 236 VKISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLHIRSDDVVLCLLPL 295
           VKISP+DVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLHIRSDDVVLCLLPL
Sbjct: 181 VKISPEDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLHIRSDDVVLCLLPL 240

Query: 296 FHIYSLNSIMMCALRVGAAILIVQKFDVNSLVELVSKYKVTFAPFVPPIVLAIAKSPAVD 355
           FHIYSLNSIMMCALRVGAAIL+VQKFDVNSLVELVSKYKVTFAPFVPPIVLAIAKSPAVD
Sbjct: 241 FHIYSLNSIMMCALRVGAAILLVQKFDVNSLVELVSKYKVTFAPFVPPIVLAIAKSPAVD 300

Query: 356 HFDMSSLRIVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFVKEGFGI 415
           HFDMSSLRIVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFVKEGFGI
Sbjct: 301 HFDMSSLRIVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFVKEGFGI 360

Query: 416 KSGGCGTIMRNSEMKILNLQTGASLPRNQTGEICIRSSQMMKGYLNDEDATKAIIDEDGW 475
           KSGGCGTIMRNSEMKILNLQTG SLPRNQTGEICIRSSQMMKGYLNDEDATKAIIDEDGW
Sbjct: 361 KSGGCGTIMRNSEMKILNLQTGESLPRNQTGEICIRSSQMMKGYLNDEDATKAIIDEDGW 420

Query: 476 LHTGDIGFVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHAHIADAAVIPMNDEVA 535
           LHTGDIGFVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHAHIADAAVIPMNDEVA
Sbjct: 421 LHTGDIGFVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHAHIADAAVIPMNDEVA 480

Query: 536 GEVPVAFIVRFDGSNITEDEIKQFISKQVVFYKRINRVFFVDSIPKSPSGKILRRQLRAL 595
           GEVPVAFIVRFDGSNITEDEIKQFISKQVVFYKRINRVFFVDSIPKSPSGKILRRQLRAL
Sbjct: 481 GEVPVAFIVRFDGSNITEDEIKQFISKQVVFYKRINRVFFVDSIPKSPSGKILRRQLRAL 540

Query: 596 LAASIS 602
           LAASIS
Sbjct: 541 LAASIS 546

BLAST of Chy2G044830 vs. ExPASy TrEMBL
Match: A0A5D3CN41 (4-coumarate--CoA ligase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold152G00670 PE=4 SV=1)

HSP 1 Score: 991.1 bits (2561), Expect = 2.0e-285
Identity = 509/550 (92.55%), Postives = 530/550 (96.36%), Query Frame = 0

Query: 56  MAAADVSSPEFIFRSKLPEIPISTHLPLHTYCFENISEFKRRPCLINAATGHTYTYGEVQ 115
           MAAAD SSPEF+FRSKLPEIPISTHLPLHTYCFENISEFK+RPCLINAATGHTYTYGEVQ
Sbjct: 1   MAAADGSSPEFLFRSKLPEIPISTHLPLHTYCFENISEFKQRPCLINAATGHTYTYGEVQ 60

Query: 116 ATSRRVAAGLHKLGIGKGDVVMLLLQNTPEFVLAFLGASYLGAAATMANPFFMPSEIAKQ 175
           ATSRRVAAGLHKLGIGKGDV+MLLLQNTPEFVLAFL ASYLGAAATMANPFFMP+EIAKQ
Sbjct: 61  ATSRRVAAGLHKLGIGKGDVIMLLLQNTPEFVLAFLAASYLGAAATMANPFFMPAEIAKQ 120

Query: 176 AVSSGAKVIITQAAFAEKVKNISQENEMIIKVIFIDDDDPP-----QFSSLIEDVSKEEE 235
           AVSSGAKVIITQ+AFAEKVK +SQ +EMIIKVIFIDDDDPP     +FSSLI+DV+KEEE
Sbjct: 121 AVSSGAKVIITQSAFAEKVKKLSQGSEMIIKVIFIDDDDPPTKGGYKFSSLIDDVAKEEE 180

Query: 236 LGMGDVKISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLHIRSDDVVL 295
           L MGDVKISPDDVVALPYSSGTTGLPKGVMLTHKG VTSVAQQVDGENPHL+IRSDDVVL
Sbjct: 181 LEMGDVKISPDDVVALPYSSGTTGLPKGVMLTHKGSVTSVAQQVDGENPHLYIRSDDVVL 240

Query: 296 CLLPLFHIYSLNSIMMCALRVGAAILIVQKFDVNSLVELVSKYKVTFAPFVPPIVLAIAK 355
           CLLPLFHIYSLNSIMMC+LRVGAAILIVQKFD+NSL+ELV KYKVTFAP VPPIVLAIAK
Sbjct: 241 CLLPLFHIYSLNSIMMCSLRVGAAILIVQKFDINSLLELVPKYKVTFAPVVPPIVLAIAK 300

Query: 356 SPAVDHFDMSSLRIVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFVK 415
           SPAVDHFDMSSLR+VLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAF K
Sbjct: 301 SPAVDHFDMSSLRMVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFAK 360

Query: 416 EGFGIKSGGCGTIMRNSEMKILNLQTGASLPRNQTGEICIRSSQMMKGYLNDEDATKAII 475
           EGFGIKSGGCGTIMRNSEMKI+  QTG SLPRN+TGEICIRS QMMKGYLNDE+ATKAII
Sbjct: 361 EGFGIKSGGCGTIMRNSEMKIVCPQTGVSLPRNRTGEICIRSFQMMKGYLNDEEATKAII 420

Query: 476 DEDGWLHTGDIGFVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHAHIADAAVIPM 535
           D+DGWLHTGDIG+VDDDDELFIVDRLKELIKYKGFQVAPAELEALLISH HIADAAVIPM
Sbjct: 421 DKDGWLHTGDIGYVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIPM 480

Query: 536 NDEVAGEVPVAFIVRFDGSNITEDEIKQFISKQVVFYKRINRVFFVDSIPKSPSGKILRR 595
           NDEVAGEVPV FIVRFDGSNITEDEIKQ+ISKQVVFYKRINRVFFVDSIPKSPSGKILRR
Sbjct: 481 NDEVAGEVPVTFIVRFDGSNITEDEIKQYISKQVVFYKRINRVFFVDSIPKSPSGKILRR 540

Query: 596 QLRALLAASI 601
           QLRALLAASI
Sbjct: 541 QLRALLAASI 550

BLAST of Chy2G044830 vs. ExPASy TrEMBL
Match: A0A1S3BWP0 (4-coumarate--CoA ligase 1-like OS=Cucumis melo OX=3656 GN=LOC103494274 PE=4 SV=1)

HSP 1 Score: 991.1 bits (2561), Expect = 2.0e-285
Identity = 509/550 (92.55%), Postives = 530/550 (96.36%), Query Frame = 0

Query: 56  MAAADVSSPEFIFRSKLPEIPISTHLPLHTYCFENISEFKRRPCLINAATGHTYTYGEVQ 115
           MAAAD SSPEF+FRSKLPEIPISTHLPLHTYCFENISEFK+RPCLINAATGHTYTYGEVQ
Sbjct: 1   MAAADGSSPEFLFRSKLPEIPISTHLPLHTYCFENISEFKQRPCLINAATGHTYTYGEVQ 60

Query: 116 ATSRRVAAGLHKLGIGKGDVVMLLLQNTPEFVLAFLGASYLGAAATMANPFFMPSEIAKQ 175
           ATSRRVAAGLHKLGIGKGDV+MLLLQNTPEFVLAFL ASYLGAAATMANPFFMP+EIAKQ
Sbjct: 61  ATSRRVAAGLHKLGIGKGDVIMLLLQNTPEFVLAFLAASYLGAAATMANPFFMPAEIAKQ 120

Query: 176 AVSSGAKVIITQAAFAEKVKNISQENEMIIKVIFIDDDDPP-----QFSSLIEDVSKEEE 235
           AVSSGAKVIITQ+AFAEKVK +SQ +EMIIKVIFIDDDDPP     +FSSLI+DV+KEEE
Sbjct: 121 AVSSGAKVIITQSAFAEKVKKLSQGSEMIIKVIFIDDDDPPTKGGYKFSSLIDDVAKEEE 180

Query: 236 LGMGDVKISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLHIRSDDVVL 295
           L MGDVKISPDDVVALPYSSGTTGLPKGVMLTHKG VTSVAQQVDGENPHL+IRSDDVVL
Sbjct: 181 LEMGDVKISPDDVVALPYSSGTTGLPKGVMLTHKGSVTSVAQQVDGENPHLYIRSDDVVL 240

Query: 296 CLLPLFHIYSLNSIMMCALRVGAAILIVQKFDVNSLVELVSKYKVTFAPFVPPIVLAIAK 355
           CLLPLFHIYSLNSIMMC+LRVGAAILIVQKFD+NSL+ELV KYKVTFAP VPPIVLAIAK
Sbjct: 241 CLLPLFHIYSLNSIMMCSLRVGAAILIVQKFDINSLLELVPKYKVTFAPVVPPIVLAIAK 300

Query: 356 SPAVDHFDMSSLRIVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFVK 415
           SPAVDHFDMSSLR+VLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAF K
Sbjct: 301 SPAVDHFDMSSLRMVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFAK 360

Query: 416 EGFGIKSGGCGTIMRNSEMKILNLQTGASLPRNQTGEICIRSSQMMKGYLNDEDATKAII 475
           EGFGIKSGGCGTIMRNSEMKI+  QTG SLPRN+TGEICIRS QMMKGYLNDE+ATKAII
Sbjct: 361 EGFGIKSGGCGTIMRNSEMKIVCPQTGVSLPRNRTGEICIRSFQMMKGYLNDEEATKAII 420

Query: 476 DEDGWLHTGDIGFVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHAHIADAAVIPM 535
           D+DGWLHTGDIG+VDDDDELFIVDRLKELIKYKGFQVAPAELEALLISH HIADAAVIPM
Sbjct: 421 DKDGWLHTGDIGYVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIPM 480

Query: 536 NDEVAGEVPVAFIVRFDGSNITEDEIKQFISKQVVFYKRINRVFFVDSIPKSPSGKILRR 595
           NDEVAGEVPV FIVRFDGSNITEDEIKQ+ISKQVVFYKRINRVFFVDSIPKSPSGKILRR
Sbjct: 481 NDEVAGEVPVTFIVRFDGSNITEDEIKQYISKQVVFYKRINRVFFVDSIPKSPSGKILRR 540

Query: 596 QLRALLAASI 601
           QLRALLAASI
Sbjct: 541 QLRALLAASI 550

BLAST of Chy2G044830 vs. ExPASy TrEMBL
Match: A0A5A7UT30 (4-coumarate--CoA ligase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G006230 PE=4 SV=1)

HSP 1 Score: 988.8 bits (2555), Expect = 1.0e-284
Identity = 508/550 (92.36%), Postives = 529/550 (96.18%), Query Frame = 0

Query: 56  MAAADVSSPEFIFRSKLPEIPISTHLPLHTYCFENISEFKRRPCLINAATGHTYTYGEVQ 115
           MAAAD SSPEF+FRSKLPEIPISTHLPLHTYCFENISEFK+RPCLINAATGHTYTYGEVQ
Sbjct: 1   MAAADGSSPEFLFRSKLPEIPISTHLPLHTYCFENISEFKQRPCLINAATGHTYTYGEVQ 60

Query: 116 ATSRRVAAGLHKLGIGKGDVVMLLLQNTPEFVLAFLGASYLGAAATMANPFFMPSEIAKQ 175
           A SRRVAAGLHKLGIGKGDV+MLLLQNTPEFVLAFL ASYLGAAATMANPFFMP+EIAKQ
Sbjct: 61  AISRRVAAGLHKLGIGKGDVIMLLLQNTPEFVLAFLAASYLGAAATMANPFFMPAEIAKQ 120

Query: 176 AVSSGAKVIITQAAFAEKVKNISQENEMIIKVIFIDDDDPP-----QFSSLIEDVSKEEE 235
           AVSSGAKVIITQ+AFAEKVK +SQ +EMIIKVIFIDDDDPP     +FSSLI+DV+KEEE
Sbjct: 121 AVSSGAKVIITQSAFAEKVKKLSQGSEMIIKVIFIDDDDPPTKGGYKFSSLIDDVAKEEE 180

Query: 236 LGMGDVKISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLHIRSDDVVL 295
           L MGDVKISPDDVVALPYSSGTTGLPKGVMLTHKG VTSVAQQVDGENPHL+IRSDDVVL
Sbjct: 181 LEMGDVKISPDDVVALPYSSGTTGLPKGVMLTHKGSVTSVAQQVDGENPHLYIRSDDVVL 240

Query: 296 CLLPLFHIYSLNSIMMCALRVGAAILIVQKFDVNSLVELVSKYKVTFAPFVPPIVLAIAK 355
           CLLPLFHIYSLNSIMMC+LRVGAAILIVQKFD+NSL+ELV KYKVTFAP VPPIVLAIAK
Sbjct: 241 CLLPLFHIYSLNSIMMCSLRVGAAILIVQKFDINSLLELVPKYKVTFAPVVPPIVLAIAK 300

Query: 356 SPAVDHFDMSSLRIVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFVK 415
           SPAVDHFDMSSLR+VLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAF K
Sbjct: 301 SPAVDHFDMSSLRMVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFAK 360

Query: 416 EGFGIKSGGCGTIMRNSEMKILNLQTGASLPRNQTGEICIRSSQMMKGYLNDEDATKAII 475
           EGFGIKSGGCGTIMRNSEMKI+  QTG SLPRN+TGEICIRS QMMKGYLNDE+ATKAII
Sbjct: 361 EGFGIKSGGCGTIMRNSEMKIVCPQTGVSLPRNRTGEICIRSFQMMKGYLNDEEATKAII 420

Query: 476 DEDGWLHTGDIGFVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHAHIADAAVIPM 535
           D+DGWLHTGDIG+VDDDDELFIVDRLKELIKYKGFQVAPAELEALLISH HIADAAVIPM
Sbjct: 421 DKDGWLHTGDIGYVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIPM 480

Query: 536 NDEVAGEVPVAFIVRFDGSNITEDEIKQFISKQVVFYKRINRVFFVDSIPKSPSGKILRR 595
           NDEVAGEVPV FIVRFDGSNITEDEIKQ+ISKQVVFYKRINRVFFVDSIPKSPSGKILRR
Sbjct: 481 NDEVAGEVPVTFIVRFDGSNITEDEIKQYISKQVVFYKRINRVFFVDSIPKSPSGKILRR 540

Query: 596 QLRALLAASI 601
           QLRALLAASI
Sbjct: 541 QLRALLAASI 550

BLAST of Chy2G044830 vs. ExPASy TrEMBL
Match: A0A6J1KTJ2 (4-coumarate--CoA ligase 2-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111497098 PE=4 SV=1)

HSP 1 Score: 849.4 bits (2193), Expect = 9.5e-243
Identity = 444/547 (81.17%), Postives = 484/547 (88.48%), Query Frame = 0

Query: 56  MAAADVSSPEFIFRSKLPEIPISTHLPLHTYCFENISEFKRRPCLINAATGHTYTYGEVQ 115
           MAAAD+ SPEFIFRSKLP+I I  HLPLHTYCFENIS+FK RPCLIN ATG TYTY EV 
Sbjct: 1   MAAADL-SPEFIFRSKLPDITIPDHLPLHTYCFENISQFKHRPCLINGATGDTYTYDEVH 60

Query: 116 ATSRRVAAGLHKLGIGKGDVVMLLLQNTPEFVLAFLGASYLGAAATMANPFFMPSEIAKQ 175
            TSRRVAAGLHKLGIGKGDV+MLLLQN+P FVLAFLGASY+GA  TMANPFF   EIAK 
Sbjct: 61  VTSRRVAAGLHKLGIGKGDVIMLLLQNSPHFVLAFLGASYIGAVVTMANPFFTLPEIAKH 120

Query: 176 AVSSGAKVIITQAAFAEKVKNISQENEMIIKVIFID--DDDPPQFSSLIEDVSKEEELGM 235
             SSGAK+IITQAAFAEKVK++  +N + IK+IFID   D    FS L +DV +E+   M
Sbjct: 121 VASSGAKLIITQAAFAEKVKSLVDKNGVSIKLIFIDPPPDGEAHFSVLTDDVKEED---M 180

Query: 236 GDVKISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLHIRSDDVVLCLL 295
            DVKISP+DVVALPYSSGTTGLPKGVMLTH+GLVTSVAQQVDGENPHL+IRSDDVVLC+L
Sbjct: 181 PDVKISPNDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPHLYIRSDDVVLCVL 240

Query: 296 PLFHIYSLNSIMMCALRVGAAILIVQKFDVNSLVELVSKYKVTFAPFVPPIVLAIAKSPA 355
           PLFHIYSLNSIMMC+LRVGAAILIVQKFD+N +VELV KYKVT AP VPPIVLAIAKSPA
Sbjct: 241 PLFHIYSLNSIMMCSLRVGAAILIVQKFDINYVVELVGKYKVTIAPLVPPIVLAIAKSPA 300

Query: 356 VDHFDMSSLRIVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFVKEGF 415
           V  ++MSS+R+VLSGAAPLGK LEDAFRAKLP  ILGQGYGMTEAGS +T+SLAF KE F
Sbjct: 301 VGGWEMSSIRMVLSGAAPLGKELEDAFRAKLPDAILGQGYGMTEAGSALTLSLAFAKERF 360

Query: 416 GIKSGGCGTIMRNSEMKILNLQTGASLPRNQTGEICIRSSQMMKGYLNDEDATKAIIDED 475
            IKSG CGT+MRNSEMKI++ QTGASLPRNQ GEI IRS  +MKGYLN+E+ATKAIIDE 
Sbjct: 361 QIKSGACGTLMRNSEMKIVSPQTGASLPRNQPGEIWIRSPHVMKGYLNNEEATKAIIDEH 420

Query: 476 GWLHTGDIGFVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHAHIADAAVIPMNDE 535
           GWLHTGDIG+VD+DDE+FIVDRLKELIKYKGFQVAPAELEALLISH HIADAAVIPM +E
Sbjct: 421 GWLHTGDIGYVDEDDEVFIVDRLKELIKYKGFQVAPAELEALLISHTHIADAAVIPMKNE 480

Query: 536 VAGEVPVAFIVRFDGSNITEDEIKQFISKQVVFYKRINRVFFVDSIPKSPSGKILRRQLR 595
           VAGEVPVAFIVR  GSNITEDEIKQ+ISKQVVFYKRI+RVFFVDSIPKSPSGKILRRQLR
Sbjct: 481 VAGEVPVAFIVRSGGSNITEDEIKQYISKQVVFYKRIHRVFFVDSIPKSPSGKILRRQLR 540

Query: 596 ALLAASI 601
            LLAA I
Sbjct: 541 ELLAARI 543

BLAST of Chy2G044830 vs. NCBI nr
Match: XP_004146311.1 (4-coumarate--CoA ligase 1 [Cucumis sativus] >KGN65444.1 hypothetical protein Csa_019996 [Cucumis sativus])

HSP 1 Score: 1034 bits (2674), Expect = 0.0
Identity = 534/546 (97.80%), Postives = 540/546 (98.90%), Query Frame = 0

Query: 56  MAAADVSSPEFIFRSKLPEIPISTHLPLHTYCFENISEFKRRPCLINAATGHTYTYGEVQ 115
           MAAAD SSPEFIFRSKLPEIPISTHLPLHTYCFENISEFK RPCLINAATGH YTYGEVQ
Sbjct: 1   MAAADASSPEFIFRSKLPEIPISTHLPLHTYCFENISEFKHRPCLINAATGHIYTYGEVQ 60

Query: 116 ATSRRVAAGLHKLGIGKGDVVMLLLQNTPEFVLAFLGASYLGAAATMANPFFMPSEIAKQ 175
           ATSRRVAAGLHKLGIGKGDV+MLLLQNTPEFVLAFLGASYLGAAATMANPFFM SEIAKQ
Sbjct: 61  ATSRRVAAGLHKLGIGKGDVIMLLLQNTPEFVLAFLGASYLGAAATMANPFFMQSEIAKQ 120

Query: 176 AVSSGAKVIITQAAFAEKVKNISQENEMIIKVIFIDDDDPPQFSSLIEDVSKEEELGMGD 235
           AVSSGAKVIITQAAFAEKVKN+SQENEMIIKVIFIDD+DPPQFSSLIEDV+KEEEL MGD
Sbjct: 121 AVSSGAKVIITQAAFAEKVKNLSQENEMIIKVIFIDDNDPPQFSSLIEDVAKEEELEMGD 180

Query: 236 VKISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLHIRSDDVVLCLLPL 295
           VKISP+DVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLHIRSDDVVLCLLPL
Sbjct: 181 VKISPEDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLHIRSDDVVLCLLPL 240

Query: 296 FHIYSLNSIMMCALRVGAAILIVQKFDVNSLVELVSKYKVTFAPFVPPIVLAIAKSPAVD 355
           FHIYSLNSIMMCALRVGAAIL+VQKFDVNSLVELVSKYKVTFAPFVPPIVLAIAKSPAVD
Sbjct: 241 FHIYSLNSIMMCALRVGAAILLVQKFDVNSLVELVSKYKVTFAPFVPPIVLAIAKSPAVD 300

Query: 356 HFDMSSLRIVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFVKEGFGI 415
           HFDMSSLRIVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFVKEGFGI
Sbjct: 301 HFDMSSLRIVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFVKEGFGI 360

Query: 416 KSGGCGTIMRNSEMKILNLQTGASLPRNQTGEICIRSSQMMKGYLNDEDATKAIIDEDGW 475
           KSGGCGTIMRNSEMKILNLQTG SLPRNQTGEICIRSSQMMKGYLNDEDATKAIIDEDGW
Sbjct: 361 KSGGCGTIMRNSEMKILNLQTGESLPRNQTGEICIRSSQMMKGYLNDEDATKAIIDEDGW 420

Query: 476 LHTGDIGFVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHAHIADAAVIPMNDEVA 535
           LHTGDIGFVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHAHIADAAVIPMNDEVA
Sbjct: 421 LHTGDIGFVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHAHIADAAVIPMNDEVA 480

Query: 536 GEVPVAFIVRFDGSNITEDEIKQFISKQVVFYKRINRVFFVDSIPKSPSGKILRRQLRAL 595
           GEVPVAFIVRFDGSNITEDEIKQFISKQVVFYKRINRVFFVDSIPKSPSGKILRRQLRAL
Sbjct: 481 GEVPVAFIVRFDGSNITEDEIKQFISKQVVFYKRINRVFFVDSIPKSPSGKILRRQLRAL 540

Query: 596 LAASIS 601
           LAASIS
Sbjct: 541 LAASIS 546

BLAST of Chy2G044830 vs. NCBI nr
Match: XP_008453615.1 (PREDICTED: 4-coumarate--CoA ligase 1-like [Cucumis melo] >TYK11846.1 4-coumarate--CoA ligase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 986 bits (2550), Expect = 0.0
Identity = 509/550 (92.55%), Postives = 530/550 (96.36%), Query Frame = 0

Query: 56  MAAADVSSPEFIFRSKLPEIPISTHLPLHTYCFENISEFKRRPCLINAATGHTYTYGEVQ 115
           MAAAD SSPEF+FRSKLPEIPISTHLPLHTYCFENISEFK+RPCLINAATGHTYTYGEVQ
Sbjct: 1   MAAADGSSPEFLFRSKLPEIPISTHLPLHTYCFENISEFKQRPCLINAATGHTYTYGEVQ 60

Query: 116 ATSRRVAAGLHKLGIGKGDVVMLLLQNTPEFVLAFLGASYLGAAATMANPFFMPSEIAKQ 175
           ATSRRVAAGLHKLGIGKGDV+MLLLQNTPEFVLAFL ASYLGAAATMANPFFMP+EIAKQ
Sbjct: 61  ATSRRVAAGLHKLGIGKGDVIMLLLQNTPEFVLAFLAASYLGAAATMANPFFMPAEIAKQ 120

Query: 176 AVSSGAKVIITQAAFAEKVKNISQENEMIIKVIFIDDDDPP-----QFSSLIEDVSKEEE 235
           AVSSGAKVIITQ+AFAEKVK +SQ +EMIIKVIFIDDDDPP     +FSSLI+DV+KEEE
Sbjct: 121 AVSSGAKVIITQSAFAEKVKKLSQGSEMIIKVIFIDDDDPPTKGGYKFSSLIDDVAKEEE 180

Query: 236 LGMGDVKISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLHIRSDDVVL 295
           L MGDVKISPDDVVALPYSSGTTGLPKGVMLTHKG VTSVAQQVDGENPHL+IRSDDVVL
Sbjct: 181 LEMGDVKISPDDVVALPYSSGTTGLPKGVMLTHKGSVTSVAQQVDGENPHLYIRSDDVVL 240

Query: 296 CLLPLFHIYSLNSIMMCALRVGAAILIVQKFDVNSLVELVSKYKVTFAPFVPPIVLAIAK 355
           CLLPLFHIYSLNSIMMC+LRVGAAILIVQKFD+NSL+ELV KYKVTFAP VPPIVLAIAK
Sbjct: 241 CLLPLFHIYSLNSIMMCSLRVGAAILIVQKFDINSLLELVPKYKVTFAPVVPPIVLAIAK 300

Query: 356 SPAVDHFDMSSLRIVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFVK 415
           SPAVDHFDMSSLR+VLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAF K
Sbjct: 301 SPAVDHFDMSSLRMVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFAK 360

Query: 416 EGFGIKSGGCGTIMRNSEMKILNLQTGASLPRNQTGEICIRSSQMMKGYLNDEDATKAII 475
           EGFGIKSGGCGTIMRNSEMKI+  QTG SLPRN+TGEICIRS QMMKGYLNDE+ATKAII
Sbjct: 361 EGFGIKSGGCGTIMRNSEMKIVCPQTGVSLPRNRTGEICIRSFQMMKGYLNDEEATKAII 420

Query: 476 DEDGWLHTGDIGFVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHAHIADAAVIPM 535
           D+DGWLHTGDIG+VDDDDELFIVDRLKELIKYKGFQVAPAELEALLISH HIADAAVIPM
Sbjct: 421 DKDGWLHTGDIGYVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIPM 480

Query: 536 NDEVAGEVPVAFIVRFDGSNITEDEIKQFISKQVVFYKRINRVFFVDSIPKSPSGKILRR 595
           NDEVAGEVPV FIVRFDGSNITEDEIKQ+ISKQVVFYKRINRVFFVDSIPKSPSGKILRR
Sbjct: 481 NDEVAGEVPVTFIVRFDGSNITEDEIKQYISKQVVFYKRINRVFFVDSIPKSPSGKILRR 540

Query: 596 QLRALLAASI 600
           QLRALLAASI
Sbjct: 541 QLRALLAASI 550

BLAST of Chy2G044830 vs. NCBI nr
Match: KAA0058290.1 (4-coumarate--CoA ligase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 984 bits (2544), Expect = 0.0
Identity = 508/550 (92.36%), Postives = 529/550 (96.18%), Query Frame = 0

Query: 56  MAAADVSSPEFIFRSKLPEIPISTHLPLHTYCFENISEFKRRPCLINAATGHTYTYGEVQ 115
           MAAAD SSPEF+FRSKLPEIPISTHLPLHTYCFENISEFK+RPCLINAATGHTYTYGEVQ
Sbjct: 1   MAAADGSSPEFLFRSKLPEIPISTHLPLHTYCFENISEFKQRPCLINAATGHTYTYGEVQ 60

Query: 116 ATSRRVAAGLHKLGIGKGDVVMLLLQNTPEFVLAFLGASYLGAAATMANPFFMPSEIAKQ 175
           A SRRVAAGLHKLGIGKGDV+MLLLQNTPEFVLAFL ASYLGAAATMANPFFMP+EIAKQ
Sbjct: 61  AISRRVAAGLHKLGIGKGDVIMLLLQNTPEFVLAFLAASYLGAAATMANPFFMPAEIAKQ 120

Query: 176 AVSSGAKVIITQAAFAEKVKNISQENEMIIKVIFIDDDDPP-----QFSSLIEDVSKEEE 235
           AVSSGAKVIITQ+AFAEKVK +SQ +EMIIKVIFIDDDDPP     +FSSLI+DV+KEEE
Sbjct: 121 AVSSGAKVIITQSAFAEKVKKLSQGSEMIIKVIFIDDDDPPTKGGYKFSSLIDDVAKEEE 180

Query: 236 LGMGDVKISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLHIRSDDVVL 295
           L MGDVKISPDDVVALPYSSGTTGLPKGVMLTHKG VTSVAQQVDGENPHL+IRSDDVVL
Sbjct: 181 LEMGDVKISPDDVVALPYSSGTTGLPKGVMLTHKGSVTSVAQQVDGENPHLYIRSDDVVL 240

Query: 296 CLLPLFHIYSLNSIMMCALRVGAAILIVQKFDVNSLVELVSKYKVTFAPFVPPIVLAIAK 355
           CLLPLFHIYSLNSIMMC+LRVGAAILIVQKFD+NSL+ELV KYKVTFAP VPPIVLAIAK
Sbjct: 241 CLLPLFHIYSLNSIMMCSLRVGAAILIVQKFDINSLLELVPKYKVTFAPVVPPIVLAIAK 300

Query: 356 SPAVDHFDMSSLRIVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFVK 415
           SPAVDHFDMSSLR+VLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAF K
Sbjct: 301 SPAVDHFDMSSLRMVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFAK 360

Query: 416 EGFGIKSGGCGTIMRNSEMKILNLQTGASLPRNQTGEICIRSSQMMKGYLNDEDATKAII 475
           EGFGIKSGGCGTIMRNSEMKI+  QTG SLPRN+TGEICIRS QMMKGYLNDE+ATKAII
Sbjct: 361 EGFGIKSGGCGTIMRNSEMKIVCPQTGVSLPRNRTGEICIRSFQMMKGYLNDEEATKAII 420

Query: 476 DEDGWLHTGDIGFVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHAHIADAAVIPM 535
           D+DGWLHTGDIG+VDDDDELFIVDRLKELIKYKGFQVAPAELEALLISH HIADAAVIPM
Sbjct: 421 DKDGWLHTGDIGYVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIPM 480

Query: 536 NDEVAGEVPVAFIVRFDGSNITEDEIKQFISKQVVFYKRINRVFFVDSIPKSPSGKILRR 595
           NDEVAGEVPV FIVRFDGSNITEDEIKQ+ISKQVVFYKRINRVFFVDSIPKSPSGKILRR
Sbjct: 481 NDEVAGEVPVTFIVRFDGSNITEDEIKQYISKQVVFYKRINRVFFVDSIPKSPSGKILRR 540

Query: 596 QLRALLAASI 600
           QLRALLAASI
Sbjct: 541 QLRALLAASI 550

BLAST of Chy2G044830 vs. NCBI nr
Match: XP_038878634.1 (4-coumarate--CoA ligase 1-like [Benincasa hispida])

HSP 1 Score: 902 bits (2332), Expect = 0.0
Identity = 466/553 (84.27%), Postives = 497/553 (89.87%), Query Frame = 0

Query: 56  MAAADVSSPEFIFRSKLPEIPISTHLPLHTYCFENISEFKRRPCLINAATGHTYTYGEVQ 115
           MAA + SSPEFIFRSKLPEI I THLPLHTY FE +SEF  RPCLINA TG T+TYGEV 
Sbjct: 1   MAATEDSSPEFIFRSKLPEISIPTHLPLHTYLFEKVSEFTNRPCLINATTGETFTYGEVH 60

Query: 116 ATSRRVAAGLHKLGIGKGDVVMLLLQNTPEFVLAFLGASYLGAAATMANPFFMPSEIAKQ 175
            TSRRVAAGLHKLGI KGDV+MLLLQNTP+FV AFLGASYLGAAAT ANPFF P+EI KQ
Sbjct: 61  ETSRRVAAGLHKLGIRKGDVIMLLLQNTPQFVFAFLGASYLGAAATTANPFFTPAEITKQ 120

Query: 176 AVSSGAKVIITQAAFAEKVKNISQENEMIIKVIFIDDDD----PPQ----FSSLIEDVSK 235
           A SS  K+IITQAAFAEKVK +SQEN+ IIKVIFIDDDD    PP     FSSL +DV+K
Sbjct: 121 AASSSIKLIITQAAFAEKVKKLSQENKAIIKVIFIDDDDDDDPPPDGGYHFSSLTDDVAK 180

Query: 236 EEELGMGDVKISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLHIRSDD 295
           EEE+ MGD K+SPDDVVALPYSSGTTGLPKGVMLTHKGLV  VAQQVDGENPH +I+SDD
Sbjct: 181 EEEMEMGDAKVSPDDVVALPYSSGTTGLPKGVMLTHKGLVAIVAQQVDGENPHFNIQSDD 240

Query: 296 VVLCLLPLFHIYSLNSIMMCALRVGAAILIVQKFDVNSLVELVSKYKVTFAPFVPPIVLA 355
           VVLC+LPLFHIYSLNSIMMC+LRVGAAILIVQKFDVN L+ L+ KYKVT AP VPPIVLA
Sbjct: 241 VVLCVLPLFHIYSLNSIMMCSLRVGAAILIVQKFDVNLLLGLIPKYKVTIAPIVPPIVLA 300

Query: 356 IAKSPAVDHFDMSSLRIVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLA 415
           I KSPAVDH DMSSLRIV+SGAAPLGKNLEDAFR KLPHVILGQGYGMTE+GS MTMSLA
Sbjct: 301 IVKSPAVDHSDMSSLRIVMSGAAPLGKNLEDAFRTKLPHVILGQGYGMTESGSAMTMSLA 360

Query: 416 FVKEGFGIKSGGCGTIMRNSEMKILNLQTGASLPRNQTGEICIRSSQMMKGYLNDEDATK 475
           F KEGF IKSGGCGTIMRN+EMKI+N QTGASLPRNQ GEICI+S Q+MKGYLNDE+ATK
Sbjct: 361 FAKEGFEIKSGGCGTIMRNTEMKIINPQTGASLPRNQAGEICIKSLQIMKGYLNDEEATK 420

Query: 476 AIIDEDGWLHTGDIGFVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHAHIADAAV 535
            IID+DGWLHTGD+GFVDDDDE+FIVDRLKELIKYKGFQVAPAELEALLISH HIADAAV
Sbjct: 421 GIIDKDGWLHTGDVGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAV 480

Query: 536 IPMNDEVAGEVPVAFIVRFDGSNITEDEIKQFISKQVVFYKRINRVFFVDSIPKSPSGKI 595
           IPM DEVAGEVPVAFI R DG+NITEDEIKQ+ISKQVVFYKRINRVFFVDSIPKSPSGKI
Sbjct: 481 IPMKDEVAGEVPVAFIARSDGTNITEDEIKQYISKQVVFYKRINRVFFVDSIPKSPSGKI 540

Query: 596 LRRQLRALLAASI 600
           LRRQLRALLAASI
Sbjct: 541 LRRQLRALLAASI 553

BLAST of Chy2G044830 vs. NCBI nr
Match: KAG6587759.1 (4-coumarate--CoA ligase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 851 bits (2198), Expect = 2.18e-306
Identity = 448/549 (81.60%), Postives = 487/549 (88.71%), Query Frame = 0

Query: 56  MAAADVSSPEFIFRSKLPEIPISTHLPLHTYCFENISEFKRRPCLINAATGHTYTYGEVQ 115
           MAAAD  SPEFIFRSKLP+I I  HLPLHTYCFENIS+FK RPCLIN ATG TYTY EV 
Sbjct: 1   MAAAD-PSPEFIFRSKLPDITIPDHLPLHTYCFENISQFKHRPCLINGATGDTYTYDEVH 60

Query: 116 ATSRRVAAGLHKLGIGKGDVVMLLLQNTPEFVLAFLGASYLGAAATMANPFFMPSEIAKQ 175
            T+RRVAAGLHKLG+GKGDV+MLLLQN+P+FVLAFLGASY+GA ATMANPFF P EIAK 
Sbjct: 61  VTARRVAAGLHKLGVGKGDVIMLLLQNSPQFVLAFLGASYIGAVATMANPFFTPPEIAKH 120

Query: 176 AVSSGAKVIITQAAFAEKVKNISQENEMIIKVIFIDDDDPP----QFSSLIEDVSKEEEL 235
           A SSGAK+IITQAAFAEKVK++  +N + IK+IFID   PP     FS L +DV +EE  
Sbjct: 121 AASSGAKLIITQAAFAEKVKSLVDKNGVSIKLIFIDP--PPYGEVHFSVLTDDVKEEE-- 180

Query: 236 GMGDVKISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLHIRSDDVVLC 295
            M DVKISP+DVVALPYSSGTTGLPKGVMLTH+GLVTSVAQQVDGENPHL+IRSDDVVLC
Sbjct: 181 -MPDVKISPNDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPHLYIRSDDVVLC 240

Query: 296 LLPLFHIYSLNSIMMCALRVGAAILIVQKFDVNSLVELVSKYKVTFAPFVPPIVLAIAKS 355
           +LPLFHIYSLNSIMMC+LRVGAAILIVQKFD+N +VELV KYKVT AP VPPIVLAIAKS
Sbjct: 241 VLPLFHIYSLNSIMMCSLRVGAAILIVQKFDINYVVELVGKYKVTIAPLVPPIVLAIAKS 300

Query: 356 PAVDHFDMSSLRIVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFVKE 415
           PAV   +MSS+R+VLSGAAPLGK LEDAFRAKLP  ILGQGYGMTEAGS +T+SLAF KE
Sbjct: 301 PAVGGTEMSSIRMVLSGAAPLGKELEDAFRAKLPDAILGQGYGMTEAGSALTLSLAFAKE 360

Query: 416 GFGIKSGGCGTIMRNSEMKILNLQTGASLPRNQTGEICIRSSQMMKGYLNDEDATKAIID 475
            F IKSG CGT+MRNS+MKI+N QTGASLPRNQ GEI IRS  +MKGYLN+E+ATKAIID
Sbjct: 361 RFPIKSGACGTLMRNSDMKIVNPQTGASLPRNQPGEIWIRSPHVMKGYLNNEEATKAIID 420

Query: 476 EDGWLHTGDIGFVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHAHIADAAVIPMN 535
           E GWLHTGDIGFVD+DDE+FIVDRLKELIKYKGFQVAPAELEALLISH HIADAAVIPM 
Sbjct: 421 EHGWLHTGDIGFVDEDDEVFIVDRLKELIKYKGFQVAPAELEALLISHTHIADAAVIPMK 480

Query: 536 DEVAGEVPVAFIVRFDGSNITEDEIKQFISKQVVFYKRINRVFFVDSIPKSPSGKILRRQ 595
           +EVAGEVPVAFIVR  GSNITEDEIKQ+ISKQVVFYKRI+RVFFVDSIPKSPSGKILRRQ
Sbjct: 481 NEVAGEVPVAFIVRSGGSNITEDEIKQYISKQVVFYKRIHRVFFVDSIPKSPSGKILRRQ 540

Query: 596 LRALLAASI 600
           LR LLAA I
Sbjct: 541 LRELLAARI 543

BLAST of Chy2G044830 vs. TAIR 10
Match: AT3G21240.1 (4-coumarate:CoA ligase 2 )

HSP 1 Score: 697.2 bits (1798), Expect = 1.2e-200
Identity = 351/537 (65.36%), Postives = 428/537 (79.70%), Query Frame = 0

Query: 63  SPEFIFRSKLPEIPISTHLPLHTYCFENISEFKRRPCLINAATGHTYTYGEVQATSRRVA 122
           S + IFRS+LP+I I  HLPLH Y FENISEF  +PCLIN  TG  YTY +V  TSR++A
Sbjct: 18  SNDVIFRSRLPDIYIPNHLPLHDYIFENISEFAAKPCLINGPTGEVYTYADVHVTSRKLA 77

Query: 123 AGLHKLGIGKGDVVMLLLQNTPEFVLAFLGASYLGAAATMANPFFMPSEIAKQAVSSGAK 182
           AGLH LG+ + DVVM+LL N+PE VL FL AS++GA  T ANPFF P+EI+KQA +S AK
Sbjct: 78  AGLHNLGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANPFFTPAEISKQAKASAAK 137

Query: 183 VIITQAAFAEKVKNISQENEMIIKVIFIDDDDPPQFSSLIEDVSKEEELGMGDV--KISP 242
           +I+TQ+ + +K+KN+  +  +I+     D D  P+      ++++ EE  +  +  KISP
Sbjct: 138 LIVTQSRYVDKIKNLQNDGVLIVTT---DSDAIPENCLRFSELTQSEEPRVDSIPEKISP 197

Query: 243 DDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLHIRSDDVVLCLLPLFHIYS 302
           +DVVALP+SSGTTGLPKGVMLTHKGLVTSVAQQVDGENP+L+   DDV+LC+LP+FHIY+
Sbjct: 198 EDVVALPFSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFNRDDVILCVLPMFHIYA 257

Query: 303 LNSIMMCALRVGAAILIVQKFDVNSLVELVSKYKVTFAPFVPPIVLAIAKSPAVDHFDMS 362
           LNSIM+C+LRVGA ILI+ KF++  L+E + + KVT A  VPPIVLAIAKSP  + +D+S
Sbjct: 258 LNSIMLCSLRVGATILIMPKFEITLLLEQIQRCKVTVAMVVPPIVLAIAKSPETEKYDLS 317

Query: 363 SLRIVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFVKEGFGIKSGGC 422
           S+R+V SGAAPLGK LEDA  AK P+  LGQGYGMTEAG V+ MSL F KE F +KSG C
Sbjct: 318 SVRMVKSGAAPLGKELEDAISAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSGAC 377

Query: 423 GTIMRNSEMKILNLQTGASLPRNQTGEICIRSSQMMKGYLNDEDATKAIIDEDGWLHTGD 482
           GT++RN+EMKIL+  TG SLPRN+ GEICIR +Q+MKGYLND  AT + ID+DGWLHTGD
Sbjct: 378 GTVVRNAEMKILDPDTGDSLPRNKPGEICIRGNQIMKGYLNDPLATASTIDKDGWLHTGD 437

Query: 483 IGFVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHAHIADAAVIPMNDEVAGEVPV 542
           +GF+DDDDELFIVDRLKELIKYKGFQVAPAELE+LLI H  I D AV+ M +E AGEVPV
Sbjct: 438 VGFIDDDDELFIVDRLKELIKYKGFQVAPAELESLLIGHPEINDVAVVAMKEEDAGEVPV 497

Query: 543 AFIVRFDGSNITEDEIKQFISKQVVFYKRINRVFFVDSIPKSPSGKILRRQLRALLA 598
           AF+VR   SNI+EDEIKQF+SKQVVFYKRIN+VFF DSIPK+PSGKILR+ LRA LA
Sbjct: 498 AFVVRSKDSNISEDEIKQFVSKQVVFYKRINKVFFTDSIPKAPSGKILRKDLRARLA 551

BLAST of Chy2G044830 vs. TAIR 10
Match: AT1G51680.1 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 696.0 bits (1795), Expect = 2.6e-200
Identity = 351/543 (64.64%), Postives = 430/543 (79.19%), Query Frame = 0

Query: 62  SSPEFIFRSKLPEIPISTHLPLHTYCFENISEFKRRPCLINAATGHTYTYGEVQATSRRV 121
           ++ + IFRSKLP+I I  HL LH Y F+NISEF  +PCLIN  TGH YTY +V   SR++
Sbjct: 19  NNSDVIFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVISRQI 78

Query: 122 AAGLHKLGIGKGDVVMLLLQNTPEFVLAFLGASYLGAAATMANPFFMPSEIAKQAVSSGA 181
           AA  HKLG+ + DVVMLLL N PEFVL+FL AS+ GA AT ANPFF P+EIAKQA +S  
Sbjct: 79  AANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASNT 138

Query: 182 KVIITQAAFAEKVKNISQENEMIIKVIFIDDDDPP-------QFSSLIEDVSKEEELGMG 241
           K+IIT+A + +K+K +  ++ ++I  + IDD++         +F+ L +  ++  E+ + 
Sbjct: 139 KLIITEARYVDKIKPLQNDDGVVI--VCIDDNESVPIPEGCLRFTELTQSTTEASEV-ID 198

Query: 242 DVKISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLHIRSDDVVLCLLP 301
            V+ISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENP+L+  SDDV+LC+LP
Sbjct: 199 SVEISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLP 258

Query: 302 LFHIYSLNSIMMCALRVGAAILIVQKFDVNSLVELVSKYKVTFAPFVPPIVLAIAKSPAV 361
           +FHIY+LNSIM+C LRVGAAILI+ KF++N L+EL+ + KVT AP VPPIVLAIAKS   
Sbjct: 259 MFHIYALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSET 318

Query: 362 DHFDMSSLRIVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFVKEGFG 421
           + +D+SS+R+V SGAAPLGK LEDA  AK P+  LGQGYGMTEAG V+ MSL F KE F 
Sbjct: 319 EKYDLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFP 378

Query: 422 IKSGGCGTIMRNSEMKILNLQTGASLPRNQTGEICIRSSQMMKGYLNDEDATKAIIDEDG 481
           +KSG CGT++RN+EMKI++  TG SL RNQ GEICIR  Q+MKGYLN+  AT   ID+DG
Sbjct: 379 VKSGACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDG 438

Query: 482 WLHTGDIGFVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHAHIADAAVIPMNDEV 541
           WLHTGDIG +DDDDELFIVDRLKELIKYKGFQVAPAELEALLI H  I D AV+ M +E 
Sbjct: 439 WLHTGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEA 498

Query: 542 AGEVPVAFIVRFDGSNITEDEIKQFISKQVVFYKRINRVFFVDSIPKSPSGKILRRQLRA 598
           AGEVPVAF+V+   S ++ED++KQF+SKQVVFYKRIN+VFF +SIPK+PSGKILR+ LRA
Sbjct: 499 AGEVPVAFVVKSKDSELSEDDVKQFVSKQVVFYKRINKVFFTESIPKAPSGKILRKDLRA 558

BLAST of Chy2G044830 vs. TAIR 10
Match: AT1G65060.1 (4-coumarate:CoA ligase 3 )

HSP 1 Score: 661.0 bits (1704), Expect = 9.2e-190
Identity = 331/562 (58.90%), Postives = 438/562 (77.94%), Query Frame = 0

Query: 46  SLHSPKLNPPMAAADVS------SPEF--IFRSKLPEIPISTHLPLHTYCFENISEFKRR 105
           +LH P+++ P   + VS      SP    IFRSKLP+I I  HLPLHTYCFE +S    +
Sbjct: 5   ALHEPQIHKPTDTSVVSDDVLPHSPPTPRIFRSKLPDIDIPNHLPLHTYCFEKLSSVSDK 64

Query: 106 PCLINAATGHTYTYGEVQATSRRVAAGLHKLGIGKGDVVMLLLQNTPEFVLAFLGASYLG 165
           PCLI  +TG +YTYGE     RRVA+GL+KLGI KGDV+M+LLQN+ EFV +F+GAS +G
Sbjct: 65  PCLIVGSTGKSYTYGETHLICRRVASGLYKLGIRKGDVIMILLQNSAEFVFSFMGASMIG 124

Query: 166 AAATMANPFFMPSEIAKQAVSSGAKVIITQAAFAEKVKNISQENEMIIKVIFIDDDDPPQ 225
           A +T ANPF+   E+ KQ  SSGAK+IIT + + +K+KN+ +     + +I  D+  P  
Sbjct: 125 AVSTTANPFYTSQELYKQLKSSGAKLIITHSQYVDKLKNLGEN----LTLITTDEPTPEN 184

Query: 226 ---FSSLIEDVSKEEELGMGDVKISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQV 285
              FS+LI D   E       V I  DD  ALP+SSGTTGLPKGV+LTHK L+TSVAQQV
Sbjct: 185 CLPFSTLITD--DETNPFQETVDIGGDDAAALPFSSGTTGLPKGVVLTHKSLITSVAQQV 244

Query: 286 DGENPHLHIRSDDVVLCLLPLFHIYSLNSIMMCALRVGAAILIVQKFDVNSLVELVSKYK 345
           DG+NP+L+++S+DV+LC+LPLFHIYSLNS+++ +LR GA +L++ KF++ +L++L+ +++
Sbjct: 245 DGDNPNLYLKSNDVILCVLPLFHIYSLNSVLLNSLRSGATVLLMHKFEIGALLDLIQRHR 304

Query: 346 VTFAPFVPPIVLAIAKSPAVDHFDMSSLRIVLSGAAPLGKNLEDAFRAKLPHVILGQGYG 405
           VT A  VPP+V+A+AK+P V+ +D+SS+R VLSGAAPLGK L+D+ R +LP  ILGQGYG
Sbjct: 305 VTIAALVPPLVIALAKNPTVNSYDLSSVRFVLSGAAPLGKELQDSLRRRLPQAILGQGYG 364

Query: 406 MTEAGSVMTMSLAFVKEGFGIKSGGCGTIMRNSEMKILNLQTGASLPRNQTGEICIRSSQ 465
           MTEAG V++MSL F KE    KSG CGT++RN+E+K+++L+T  SL  NQ GEICIR  Q
Sbjct: 365 MTEAGPVLSMSLGFAKEPIPTKSGSCGTVVRNAELKVVHLETRLSLGYNQPGEICIRGQQ 424

Query: 466 MMKGYLNDEDATKAIIDEDGWLHTGDIGFVDDDDELFIVDRLKELIKYKGFQVAPAELEA 525
           +MK YLND +AT A IDE+GWLHTGDIG+VD+DDE+FIVDRLKE+IK+KGFQV PAELE+
Sbjct: 425 IMKEYLNDPEATSATIDEEGWLHTGDIGYVDEDDEIFIVDRLKEVIKFKGFQVPPAELES 484

Query: 526 LLISHAHIADAAVIPMNDEVAGEVPVAFIVRFDGSNITEDEIKQFISKQVVFYKRINRVF 585
           LLI+H  IADAAV+P NDEVAGEVPVAF+VR +G++ITE+++K++++KQVVFYKR+++VF
Sbjct: 485 LLINHHSIADAAVVPQNDEVAGEVPVAFVVRSNGNDITEEDVKEYVAKQVVFYKRLHKVF 544

Query: 586 FVDSIPKSPSGKILRRQLRALL 597
           FV SIPKSPSGKILR+ L+A L
Sbjct: 545 FVASIPKSPSGKILRKDLKAKL 560

BLAST of Chy2G044830 vs. TAIR 10
Match: AT1G51680.3 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 643.7 bits (1659), Expect = 1.5e-184
Identity = 325/510 (63.73%), Postives = 400/510 (78.43%), Query Frame = 0

Query: 62  SSPEFIFRSKLPEIPISTHLPLHTYCFENISEFKRRPCLINAATGHTYTYGEVQATSRRV 121
           ++ + IFRSKLP+I I  HL LH Y F+NISEF  +PCLIN  TGH YTY +V   SR++
Sbjct: 19  NNSDVIFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVISRQI 78

Query: 122 AAGLHKLGIGKGDVVMLLLQNTPEFVLAFLGASYLGAAATMANPFFMPSEIAKQAVSSGA 181
           AA  HKLG+ + DVVMLLL N PEFVL+FL AS+ GA AT ANPFF P+EIAKQA +S  
Sbjct: 79  AANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASNT 138

Query: 182 KVIITQAAFAEKVKNISQENEMIIKVIFIDDDDPP-------QFSSLIEDVSKEEELGMG 241
           K+IIT+A + +K+K +  ++ ++I  + IDD++         +F+ L +  ++  E+ + 
Sbjct: 139 KLIITEARYVDKIKPLQNDDGVVI--VCIDDNESVPIPEGCLRFTELTQSTTEASEV-ID 198

Query: 242 DVKISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLHIRSDDVVLCLLP 301
            V+ISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENP+L+  SDDV+LC+LP
Sbjct: 199 SVEISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLP 258

Query: 302 LFHIYSLNSIMMCALRVGAAILIVQKFDVNSLVELVSKYKVTFAPFVPPIVLAIAKSPAV 361
           +FHIY+LNSIM+C LRVGAAILI+ KF++N L+EL+ + KVT AP VPPIVLAIAKS   
Sbjct: 259 MFHIYALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSET 318

Query: 362 DHFDMSSLRIVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFVKEGFG 421
           + +D+SS+R+V SGAAPLGK LEDA  AK P+  LGQGYGMTEAG V+ MSL F KE F 
Sbjct: 319 EKYDLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFP 378

Query: 422 IKSGGCGTIMRNSEMKILNLQTGASLPRNQTGEICIRSSQMMKGYLNDEDATKAIIDEDG 481
           +KSG CGT++RN+EMKI++  TG SL RNQ GEICIR  Q+MKGYLN+  AT   ID+DG
Sbjct: 379 VKSGACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDG 438

Query: 482 WLHTGDIGFVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHAHIADAAVIPMNDEV 541
           WLHTGDIG +DDDDELFIVDRLKELIKYKGFQVAPAELEALLI H  I D AV+ M +E 
Sbjct: 439 WLHTGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEA 498

Query: 542 AGEVPVAFIVRFDGSNITEDEIKQFISKQV 565
           AGEVPVAF+V+   S ++ED++KQF+SKQV
Sbjct: 499 AGEVPVAFVVKSKDSELSEDDVKQFVSKQV 525

BLAST of Chy2G044830 vs. TAIR 10
Match: AT3G21230.1 (4-coumarate:CoA ligase 5 )

HSP 1 Score: 627.1 bits (1616), Expect = 1.5e-179
Identity = 320/549 (58.29%), Postives = 412/549 (75.05%), Query Frame = 0

Query: 63  SPEFIFRSKLPEIPISTHLPLHTYCFENIS----EFKRRPCLINAATGHTYTYGEVQATS 122
           S +FIFRSKLP+I I  HLPL  Y F+  S          C+I+ ATG   TY +VQ   
Sbjct: 24  SHDFIFRSKLPDIFIPNHLPLTDYVFQRFSGDGDGDSSTTCIIDGATGRILTYADVQTNM 83

Query: 123 RRVAAGLHKLGIGKGDVVMLLLQNTPEFVLAFLGASYLGAAATMANPFFMPSEIAKQAVS 182
           RR+AAG+H+LGI  GDVVMLLL N+PEF L+FL  +YLGA +T ANPF+   EIAKQA +
Sbjct: 84  RRIAAGIHRLGIRHGDVVMLLLPNSPEFALSFLAVAYLGAVSTTANPFYTQPEIAKQAKA 143

Query: 183 SGAKVIITQAAFAEKVKNISQENEMIIKVIFIDDDDPPQFSS------LIEDVSKEEELG 242
           S AK+IIT+    +K+ N+  +N+ ++ V   DD D    SS         ++++ +E  
Sbjct: 144 SAAKMIITKKCLVDKLTNL--KNDGVLIVCLDDDGDNGVVSSSDDGCVSFTELTQADETE 203

Query: 243 MGDVKISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLHIRSDDVVLCL 302
           +   KISP+D VA+PYSSGTTGLPKGVM+THKGLVTS+AQ+VDGENP+L+  ++DV+LC 
Sbjct: 204 LLKPKISPEDTVAMPYSSGTTGLPKGVMITHKGLVTSIAQKVDGENPNLNFTANDVILCF 263

Query: 303 LPLFHIYSLNSIMMCALRVGAAILIVQKFDVNSLVELVSKYKVTFAPFVPPIVLAIAKSP 362
           LP+FHIY+L+++M+ A+R GAA+LIV +F++N ++EL+ +YKVT  P  PP+VLA  KSP
Sbjct: 264 LPMFHIYALDALMLSAMRTGAALLIVPRFELNLVMELIQRYKVTVVPVAPPVVLAFIKSP 323

Query: 363 AVDHFDMSSLRIVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFVKEG 422
             + +D+SS+RI+LSGAA L K LEDA R K P+ I GQGYGMTE+G+V   SLAF K  
Sbjct: 324 ETERYDLSSVRIMLSGAATLKKELEDAVRLKFPNAIFGQGYGMTESGTV-AKSLAFAKNP 383

Query: 423 FGIKSGGCGTIMRNSEMKILNLQTGASLPRNQTGEICIRSSQMMKGYLNDEDATKAIIDE 482
           F  KSG CGT++RN+EMK+++ +TG SLPRN++GEIC+R  Q+MKGYLND +AT   ID+
Sbjct: 384 FKTKSGACGTVIRNAEMKVVDTETGISLPRNKSGEICVRGHQLMKGYLNDPEATARTIDK 443

Query: 483 DGWLHTGDIGFVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHAHIADAAVIPMND 542
           DGWLHTGDIGFVDDDDE+FIVDRLKELIK+KG+QVAPAELEALLISH  I DAAV+ M D
Sbjct: 444 DGWLHTGDIGFVDDDDEIFIVDRLKELIKFKGYQVAPAELEALLISHPSIDDAAVVAMKD 503

Query: 543 EVAGEVPVAFIVRFDGSNITEDEIKQFISKQVVFYKRINRVFFVDSIPKSPSGKILRRQL 602
           EVA EVPVAF+ R  GS +TED++K +++KQVV YKRI  VFF++ IPK+ SGKILR+ L
Sbjct: 504 EVADEVPVAFVARSQGSQLTEDDVKSYVNKQVVHYKRIKMVFFIEVIPKAVSGKILRKDL 563