Chy2G040510 (gene) Cucumber (hystrix) v1

Overview
NameChy2G040510
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionProtein kinase domain-containing protein
LocationchrH02: 19331307 .. 19332350 (-)
RNA-Seq ExpressionChy2G040510
SyntenyChy2G040510
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCGACATGATCGTGAACAGCCAATACCAAATCCTCGAGGAGATCGGAAGAGGAAGGTTCGGAATCATCACTCGTTGCTTTTGTCCAATCTCCAACAAGTTCTTCGCCTGCAAAACCATCTCTAAAAACGATCTCAACGATGAAACTGACAAAGAATGCCTTGTCAAAGAACCCAAAATCATGGCTCTTCTCCCTCCCCATCCCAACATTCTTCAGTTAATCGACGTTCTCGAGAATGACCATTACCTCTGTTTGATTTCCGACCTCTGTGAATCCCTCTCCCTTTACGATCGGATCATCCGTCGTCCCTTTTCTGAATCCGAAGCTGCAGTCGTTATGAAGCAGCTTCTCCAAGCCCTCGCCCACTGTCATTCTCATGGCGTCGTTCATCGCGATGTCAAGCCTGACAATGTGCTCTTCGATTCCAGGAACAATTTGAAACTCATCGATTTCGGATCGGCTGAGTGGTGTGATGAAGATGGATTTATGTACGGTGTCGTCGGAACGCCTTATTACGTTGCGCCGGAAGTTTTGAGAGGATCTGAGTACGGTCGGAAGGTCGACGTGTGGAGCGCCGGTGTGATTCTTTACACGATGTTGGCCGGATTTCCCCCGTTCTACGGTGAATCTGCGGCGGAGGTCTTTGAGGCTGTTCTTAGAGGAAATCTCAGATTTCCGACTAGGGTTTTTAGGTCGGTTTCTTCAGCGGCTAAGGATCTGATGAAGAAGATGATCTGTCGAGATGTTTCTAGAAGATTTTCAGCCGAACAAGCTCTCAGTAAGTCTAATCCTTATATTGAATCAAACCATTTTATGTTCTTGAGCTGGTTGTTCTGGCAACTGTTTATTGATTGATTCCATATCTTTTTAGCAAATGTTGAACCATTTGAAGAAACCCCAAAACCCCAAAACAAGTGAAAATTCATTGCATGTTTCAAAACTAGTGAATCTATTTGTTTTCTTATGGGAGTTGGAAACTGTTGTTTTGCAGGACACTCATGGATATTGAGTGGAGGAGAAGGAACATCTTCAGTTGAATAA

mRNA sequence

ATGGCCGACATGATCGTGAACAGCCAATACCAAATCCTCGAGGAGATCGGAAGAGGAAGGTTCGGAATCATCACTCGTTGCTTTTGTCCAATCTCCAACAAGTTCTTCGCCTGCAAAACCATCTCTAAAAACGATCTCAACGATGAAACTGACAAAGAATGCCTTGTCAAAGAACCCAAAATCATGGCTCTTCTCCCTCCCCATCCCAACATTCTTCAGTTAATCGACGTTCTCGAGAATGACCATTACCTCTGTTTGATTTCCGACCTCTGTGAATCCCTCTCCCTTTACGATCGGATCATCCGTCGTCCCTTTTCTGAATCCGAAGCTGCAGTCGTTATGAAGCAGCTTCTCCAAGCCCTCGCCCACTGTCATTCTCATGGCGTCGTTCATCGCGATGTCAAGCCTGACAATGTGCTCTTCGATTCCAGGAACAATTTGAAACTCATCGATTTCGGATCGGCTGAGTGGTGTGATGAAGATGGATTTATGTACGGTGTCGTCGGAACGCCTTATTACGTTGCGCCGGAAGTTTTGAGAGGATCTGAGTACGGTCGGAAGGTCGACGTGTGGAGCGCCGGTGTGATTCTTTACACGATGTTGGCCGGATTTCCCCCGTTCTACGGTGAATCTGCGGCGGAGGTCTTTGAGGCTGTTCTTAGAGGAAATCTCAGATTTCCGACTAGGGTTTTTAGGTCGGTTTCTTCAGCGGCTAAGGATCTGATGAAGAAGATGATCTGTCGAGATGTTTCTAGAAGATTTTCAGCCGAACAAGCTCTCAGACACTCATGGATATTGAGTGGAGGAGAAGGAACATCTTCAGTTGAATAA

Coding sequence (CDS)

ATGGCCGACATGATCGTGAACAGCCAATACCAAATCCTCGAGGAGATCGGAAGAGGAAGGTTCGGAATCATCACTCGTTGCTTTTGTCCAATCTCCAACAAGTTCTTCGCCTGCAAAACCATCTCTAAAAACGATCTCAACGATGAAACTGACAAAGAATGCCTTGTCAAAGAACCCAAAATCATGGCTCTTCTCCCTCCCCATCCCAACATTCTTCAGTTAATCGACGTTCTCGAGAATGACCATTACCTCTGTTTGATTTCCGACCTCTGTGAATCCCTCTCCCTTTACGATCGGATCATCCGTCGTCCCTTTTCTGAATCCGAAGCTGCAGTCGTTATGAAGCAGCTTCTCCAAGCCCTCGCCCACTGTCATTCTCATGGCGTCGTTCATCGCGATGTCAAGCCTGACAATGTGCTCTTCGATTCCAGGAACAATTTGAAACTCATCGATTTCGGATCGGCTGAGTGGTGTGATGAAGATGGATTTATGTACGGTGTCGTCGGAACGCCTTATTACGTTGCGCCGGAAGTTTTGAGAGGATCTGAGTACGGTCGGAAGGTCGACGTGTGGAGCGCCGGTGTGATTCTTTACACGATGTTGGCCGGATTTCCCCCGTTCTACGGTGAATCTGCGGCGGAGGTCTTTGAGGCTGTTCTTAGAGGAAATCTCAGATTTCCGACTAGGGTTTTTAGGTCGGTTTCTTCAGCGGCTAAGGATCTGATGAAGAAGATGATCTGTCGAGATGTTTCTAGAAGATTTTCAGCCGAACAAGCTCTCAGACACTCATGGATATTGAGTGGAGGAGAAGGAACATCTTCAGTTGAATAA

Protein sequence

MADMIVNSQYQILEEIGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPKIMALLPPHPNILQLIDVLENDHYLCLISDLCESLSLYDRIIRRPFSESEAAVVMKQLLQALAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLRGSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKDLMKKMICRDVSRRFSAEQALRHSWILSGGEGTSSVE*
Homology
BLAST of Chy2G040510 vs. ExPASy Swiss-Prot
Match: Q9SPK4 (Phosphoenolpyruvate carboxylase kinase 1 OS=Arabidopsis thaliana OX=3702 GN=PPCK1 PE=1 SV=1)

HSP 1 Score: 336.7 bits (862), Expect = 2.6e-91
Identity = 161/266 (60.53%), Postives = 202/266 (75.94%), Query Frame = 0

Query: 8   SQYQILEEIGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPKIMALLPP 67
           ++YQI EEIGRGRFG ++R + P +  FFACKTI K  L+D+ D+ CL  EPK+MALL  
Sbjct: 13  NKYQICEEIGRGRFGTVSRVYAPATGDFFACKTIDKASLSDDLDRACLDNEPKLMALLSY 72

Query: 68  HPNILQLIDVLENDHYLCLISDLCE-SLSLYDRIIRR-PFSESEAAVVMKQLLQALAHCH 127
           HPNI+Q+ D+++ D  L +  +L   S+S+YDR++    F E + A   KQ+LQAL+HCH
Sbjct: 73  HPNIVQIHDLIDTDSTLSIFMELVHPSVSIYDRLVSSGTFFEPQTASFAKQILQALSHCH 132

Query: 128 SHGVVHRDVKPDNVLFDSRNN-LKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLRGSEY 187
            +GVVHRD+KP+N+L D RN+ +K+ DFGS  W  E     GVVGTPYYVAPEVL G  Y
Sbjct: 133 RYGVVHRDIKPENILVDLRNDTVKICDFGSGIWLGEGETTEGVVGTPYYVAPEVLMGYSY 192

Query: 188 GRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKDLMKK 247
           G KVD+WSAGV+LYTMLAG PPFYGE+A E+FEAVLRGNLRFPT++FR VSS AKD ++K
Sbjct: 193 GEKVDLWSAGVVLYTMLAGTPPFYGETAEEIFEAVLRGNLRFPTKIFRGVSSMAKDFLRK 252

Query: 248 MICRDVSRRFSAEQALRHSWILSGGE 271
           +IC+D SRRFSAEQALRH WI   GE
Sbjct: 253 LICKDASRRFSAEQALRHPWIQRAGE 278

BLAST of Chy2G040510 vs. ExPASy Swiss-Prot
Match: Q93VK0 (Phosphoenolpyruvate carboxylase kinase 2 OS=Arabidopsis thaliana OX=3702 GN=PPCK2 PE=1 SV=2)

HSP 1 Score: 324.3 bits (830), Expect = 1.3e-87
Identity = 158/268 (58.96%), Postives = 202/268 (75.37%), Query Frame = 0

Query: 6   VNSQYQILEEIGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPKIMALL 65
           + + YQ+ +EIGRGRFG ITRCF P + +F+ACKTI K  L D  D+EC+  EP+IMA+L
Sbjct: 7   LENNYQLCDEIGRGRFGTITRCFSPATKEFYACKTIDKRVLIDALDRECIETEPRIMAML 66

Query: 66  PPHPNILQLIDVLENDHYLCLISDLCE-SLSLYDRIIRR--PFSESEAAVVMKQLLQALA 125
           PPHPNI+++ D+ E +  L ++ +L +  +++YDR+I      SESE+A   KQ+L ALA
Sbjct: 67  PPHPNIIRIFDLYETEDSLAIVMELVDPPMTIYDRLISAGGRLSESESASYAKQILSALA 126

Query: 126 HCHSHGVVHRDVKPDNVLFD-SRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLRG 185
           HCH   VVHRDVKPDNVL D     +KL DFGSA W   +    GVVGTPYYVAPEV+ G
Sbjct: 127 HCHRCDVVHRDVKPDNVLVDLVSGGVKLCDFGSAVWLGGE-TAEGVVGTPYYVAPEVVMG 186

Query: 186 SEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKDL 245
            +Y  KVD+WSAGV++YTMLAG PPF GE+A ++FE++LRGNLRFP + F SVSS AKDL
Sbjct: 187 RKYDEKVDIWSAGVVIYTMLAGEPPFNGETAEDIFESILRGNLRFPPKKFGSVSSEAKDL 246

Query: 246 MKKMICRDVSRRFSAEQALRHSWILSGG 270
           ++KMICRDVSRRFSAE ALRHSW+++ G
Sbjct: 247 LRKMICRDVSRRFSAEDALRHSWMMNVG 273

BLAST of Chy2G040510 vs. ExPASy Swiss-Prot
Match: Q42438 (Calcium-dependent protein kinase 8 OS=Arabidopsis thaliana OX=3702 GN=CPK8 PE=1 SV=1)

HSP 1 Score: 220.7 bits (561), Expect = 2.1e-56
Identity = 115/264 (43.56%), Postives = 164/264 (62.12%), Query Frame = 0

Query: 6   VNSQYQILEEIGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPKIMALL 65
           ++  Y +  E+GRG FGI   C    + + +ACK+ISK  L    D E + +E +IM  +
Sbjct: 53  ISLMYDLGREVGRGEFGITYLCTDIKTGEKYACKSISKKKLRTAVDIEDVRREVEIMKHM 112

Query: 66  PPHPNILQLIDVLENDHYLCLISDLCESLSLYDRIIRR-PFSESEAAVVMKQLLQALAHC 125
           P HPNI+ L D  E+D  + ++ +LCE   L+DRI+ R  ++E  AA VMK +L+ +  C
Sbjct: 113 PRHPNIVSLKDAFEDDDAVHIVMELCEGGELFDRIVARGHYTERAAAAVMKTILEVVQIC 172

Query: 126 HSHGVVHRDVKPDNVLFDSRNN---LKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLRG 185
           H HGV+HRD+KP+N LF ++     LK IDFG + +         +VG+PYY+APEVLR 
Sbjct: 173 HKHGVMHRDLKPENFLFANKKETSALKAIDFGLSVFFKPGEGFNEIVGSPYYMAPEVLR- 232

Query: 186 SEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKDL 245
             YG +VD+WSAGVILY +L G PPF+ E+   V +A++R  + F    +  VS  AKDL
Sbjct: 233 RNYGPEVDIWSAGVILYILLCGVPPFWAETEQGVAQAIIRSVIDFKRDPWPRVSETAKDL 292

Query: 246 MKKMICRDVSRRFSAEQALRHSWI 266
           ++KM+  D  +R SA Q L HSWI
Sbjct: 293 VRKMLEPDPKKRLSAAQVLEHSWI 315

BLAST of Chy2G040510 vs. ExPASy Swiss-Prot
Match: P93759 (Calcium-dependent protein kinase 14 OS=Arabidopsis thaliana OX=3702 GN=CPK14 PE=2 SV=1)

HSP 1 Score: 219.5 bits (558), Expect = 4.6e-56
Identity = 113/267 (42.32%), Postives = 169/267 (63.30%), Query Frame = 0

Query: 6   VNSQYQILEEIGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPKIMALL 65
           +  +Y++  E+GRG FG+   C    + + FACK+I K  L    D E + +E +IM  +
Sbjct: 50  IKQKYKLGRELGRGEFGVTYLCTEIETGEIFACKSILKKKLKTSIDIEDVKREVEIMRQM 109

Query: 66  PPHPNILQLIDVLENDHYLCLISDLCESLSLYDRIIRR-PFSESEAAVVMKQLLQALAHC 125
           P HPNI+ L +  E+D  + L+ +LCE   L+DRI+ R  ++E  AA V+K +++ +  C
Sbjct: 110 PEHPNIVTLKETYEDDKAVHLVMELCEGGELFDRIVARGHYTERAAASVIKTIIEVVQMC 169

Query: 126 HSHGVVHRDVKPDNVLFDSRN---NLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLRG 185
           H HGV+HRD+KP+N LF ++    +LK IDFG + +         +VG+PYY+APEVLR 
Sbjct: 170 HKHGVMHRDLKPENFLFANKKETASLKAIDFGLSVFFKPGERFNEIVGSPYYMAPEVLRR 229

Query: 186 SEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKDL 245
           S YG+++D+WSAGVILY +L G PPF+ E+   V +A+L+  + F    +  VS  AKDL
Sbjct: 230 S-YGQEIDIWSAGVILYILLCGVPPFWAETEHGVAKAILKSVIDFKRDPWPKVSDNAKDL 289

Query: 246 MKKMICRDVSRRFSAEQALRHSWILSG 269
           +KKM+  D  RR +A+Q L H WI +G
Sbjct: 290 IKKMLHPDPRRRLTAQQVLDHPWIQNG 315

BLAST of Chy2G040510 vs. ExPASy Swiss-Prot
Match: P28582 (Calcium-dependent protein kinase OS=Daucus carota OX=4039 PE=2 SV=2)

HSP 1 Score: 219.5 bits (558), Expect = 4.6e-56
Identity = 110/271 (40.59%), Postives = 174/271 (64.21%), Query Frame = 0

Query: 6   VNSQYQILEEIGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPKIMALL 65
           +  +Y + +E+GRG+FG + +C    S + +ACK+I K  L  + DKE + +E +I+  L
Sbjct: 77  IRGKYTLGKELGRGQFGCVYQCTENSSGQLYACKSILKRKLVSKNDKEDIKREIQILQHL 136

Query: 66  PPHPNILQLIDVLENDHYLCLISDLCESLSLYDRIIRR-PFSESEAAVVMKQLLQALAHC 125
              PNI++   V E+   + L+ +LC    L+DRII +  +SE  AA + +Q++  +  C
Sbjct: 137 SGQPNIVEFKGVFEDRQSVHLVMELCAGGELFDRIIAQGHYSERAAATICRQIVNVVHVC 196

Query: 126 HSHGVVHRDVKPDNVLFDSRNN---LKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLRG 185
           H  GV+HRD+KP+N L  S++    LK  DFG + + +E      +VG+ YYVAPEVLR 
Sbjct: 197 HFMGVMHRDLKPENFLLSSKDKDAMLKATDFGLSVFIEEGKVYRNIVGSAYYVAPEVLRR 256

Query: 186 SEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKDL 245
           S YG+++D+WSAGVILY +L+G PPF+ E+   +F+A+L G + F +  + SVS++AKDL
Sbjct: 257 S-YGKEIDIWSAGVILYILLSGVPPFWAENEKGIFDAILEGVIDFESEPWPSVSNSAKDL 316

Query: 246 MKKMICRDVSRRFSAEQALRHSWILSGGEGT 273
           ++KM+ +D  RR ++ Q L H W+  GGE +
Sbjct: 317 VRKMLTQDPRRRITSAQVLDHPWMREGGEAS 346

BLAST of Chy2G040510 vs. ExPASy TrEMBL
Match: A0A0A0M3G7 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G690150 PE=3 SV=1)

HSP 1 Score: 558.9 bits (1439), Expect = 1.2e-155
Identity = 272/276 (98.55%), Postives = 273/276 (98.91%), Query Frame = 0

Query: 1   MADMIVNSQYQILEEIGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPK 60
           MADMIVNSQYQILEE+GRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPK
Sbjct: 1   MADMIVNSQYQILEELGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPK 60

Query: 61  IMALLPPHPNILQLIDVLENDHYLCLISDLCESLSLYDRIIRRPFSESEAAVVMKQLLQA 120
           IMALLP HPNILQLIDVLENDHYLCLISDLCESLSLYDRIIRRPFSESEAAVVMKQLLQA
Sbjct: 61  IMALLPSHPNILQLIDVLENDHYLCLISDLCESLSLYDRIIRRPFSESEAAVVMKQLLQA 120

Query: 121 LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLR 180
           LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLR
Sbjct: 121 LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLR 180

Query: 181 GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240
           GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD
Sbjct: 181 GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240

Query: 241 LMKKMICRDVSRRFSAEQALRHSWILSGGEGTSSVE 277
           LMKKMICRDVSRRFSAEQALRH WILSGGE TSSVE
Sbjct: 241 LMKKMICRDVSRRFSAEQALRHPWILSGGEATSSVE 276

BLAST of Chy2G040510 vs. ExPASy TrEMBL
Match: A0A5A7TYV2 (Phosphoenolpyruvate carboxylase kinase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold403G00970 PE=3 SV=1)

HSP 1 Score: 555.1 bits (1429), Expect = 1.7e-154
Identity = 271/276 (98.19%), Postives = 271/276 (98.19%), Query Frame = 0

Query: 1   MADMIVNSQYQILEEIGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPK 60
           MADMIVNSQYQILEEIGRGRFGIITRCFCPISNKFFACKTISK DL DETDKECLVKEPK
Sbjct: 1   MADMIVNSQYQILEEIGRGRFGIITRCFCPISNKFFACKTISKTDLIDETDKECLVKEPK 60

Query: 61  IMALLPPHPNILQLIDVLENDHYLCLISDLCESLSLYDRIIRRPFSESEAAVVMKQLLQA 120
           IMALLPPHPNILQLIDVLENDHYLCLISDLCE LSLYDRIIRRPFSESEAAVVMKQLLQA
Sbjct: 61  IMALLPPHPNILQLIDVLENDHYLCLISDLCEPLSLYDRIIRRPFSESEAAVVMKQLLQA 120

Query: 121 LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLR 180
           LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLR
Sbjct: 121 LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLR 180

Query: 181 GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240
           GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD
Sbjct: 181 GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240

Query: 241 LMKKMICRDVSRRFSAEQALRHSWILSGGEGTSSVE 277
           LMKKMICRDVSRR SAEQALRH WILSGGEGTSSVE
Sbjct: 241 LMKKMICRDVSRRLSAEQALRHPWILSGGEGTSSVE 276

BLAST of Chy2G040510 vs. ExPASy TrEMBL
Match: A0A1S3CHK9 (phosphoenolpyruvate carboxylase kinase 1 OS=Cucumis melo OX=3656 GN=LOC103501000 PE=3 SV=1)

HSP 1 Score: 555.1 bits (1429), Expect = 1.7e-154
Identity = 271/276 (98.19%), Postives = 271/276 (98.19%), Query Frame = 0

Query: 1   MADMIVNSQYQILEEIGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPK 60
           MADMIVNSQYQILEEIGRGRFGIITRCFCPISNKFFACKTISK DL DETDKECLVKEPK
Sbjct: 1   MADMIVNSQYQILEEIGRGRFGIITRCFCPISNKFFACKTISKTDLIDETDKECLVKEPK 60

Query: 61  IMALLPPHPNILQLIDVLENDHYLCLISDLCESLSLYDRIIRRPFSESEAAVVMKQLLQA 120
           IMALLPPHPNILQLIDVLENDHYLCLISDLCE LSLYDRIIRRPFSESEAAVVMKQLLQA
Sbjct: 61  IMALLPPHPNILQLIDVLENDHYLCLISDLCEPLSLYDRIIRRPFSESEAAVVMKQLLQA 120

Query: 121 LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLR 180
           LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLR
Sbjct: 121 LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLR 180

Query: 181 GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240
           GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD
Sbjct: 181 GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240

Query: 241 LMKKMICRDVSRRFSAEQALRHSWILSGGEGTSSVE 277
           LMKKMICRDVSRR SAEQALRH WILSGGEGTSSVE
Sbjct: 241 LMKKMICRDVSRRLSAEQALRHPWILSGGEGTSSVE 276

BLAST of Chy2G040510 vs. ExPASy TrEMBL
Match: A0A6J1JGI4 (phosphoenolpyruvate carboxylase kinase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111485498 PE=3 SV=1)

HSP 1 Score: 526.9 bits (1356), Expect = 5.0e-146
Identity = 252/276 (91.30%), Postives = 266/276 (96.38%), Query Frame = 0

Query: 1   MADMIVNSQYQILEEIGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPK 60
           M DMIVNSQY+ILEEIGRGRFG ITRCFCPISNKFFACKTI+K+DL DETDKECL KEPK
Sbjct: 1   MGDMIVNSQYEILEEIGRGRFGTITRCFCPISNKFFACKTIAKSDLIDETDKECLEKEPK 60

Query: 61  IMALLPPHPNILQLIDVLENDHYLCLISDLCESLSLYDRIIRRPFSESEAAVVMKQLLQA 120
           IMALLPPHPNILQLIDV END YLCLIS+LC+S+SLYDRIIRRPFSESEAA+VMKQLLQA
Sbjct: 61  IMALLPPHPNILQLIDVFENDDYLCLISELCDSVSLYDRIIRRPFSESEAAIVMKQLLQA 120

Query: 121 LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLR 180
           LAHCH HGVVHRDVKPDNVLFDSR+NLKLIDFGSAEWCD+DGF +GVVGTPYY+APEVLR
Sbjct: 121 LAHCHYHGVVHRDVKPDNVLFDSRDNLKLIDFGSAEWCDKDGFTFGVVGTPYYIAPEVLR 180

Query: 181 GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240
           GSEYG+KVDVWSAGVILYTMLAGFPPFYG+SAAEVFEAVLRGNLRFP RVFRS+SSAAKD
Sbjct: 181 GSEYGQKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPPRVFRSISSAAKD 240

Query: 241 LMKKMICRDVSRRFSAEQALRHSWILSGGEGTSSVE 277
           LMKKMICRDVSRRFSAEQALRH WILSGGEGTSSVE
Sbjct: 241 LMKKMICRDVSRRFSAEQALRHPWILSGGEGTSSVE 276

BLAST of Chy2G040510 vs. ExPASy TrEMBL
Match: A0A6J1HBL3 (phosphoenolpyruvate carboxylase kinase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111461799 PE=3 SV=1)

HSP 1 Score: 524.2 bits (1349), Expect = 3.2e-145
Identity = 250/276 (90.58%), Postives = 265/276 (96.01%), Query Frame = 0

Query: 1   MADMIVNSQYQILEEIGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPK 60
           M DMIVNSQY+ILEEIGRGRFG ITRCFCPISNKFFACKTI+K+DL DETDKECL KEPK
Sbjct: 1   MGDMIVNSQYEILEEIGRGRFGTITRCFCPISNKFFACKTIAKSDLIDETDKECLEKEPK 60

Query: 61  IMALLPPHPNILQLIDVLENDHYLCLISDLCESLSLYDRIIRRPFSESEAAVVMKQLLQA 120
           IMALLPPHPNILQLIDV END YLCLIS+LC+S+SLYDRIIRRPFSESEAA+VMKQLLQA
Sbjct: 61  IMALLPPHPNILQLIDVFENDDYLCLISELCDSVSLYDRIIRRPFSESEAAIVMKQLLQA 120

Query: 121 LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLR 180
           LAHCH HGVVHRDVKPDNVLFDSR+NLKLIDFGSAEWCD+DGF +GVVGTPYY+APEVLR
Sbjct: 121 LAHCHYHGVVHRDVKPDNVLFDSRDNLKLIDFGSAEWCDKDGFTFGVVGTPYYIAPEVLR 180

Query: 181 GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240
           GSEYG+KVDVWSAGVILYTMLAGFPPFYG+SAAEVFEAVLRGNLRFP RVFRS+S AAKD
Sbjct: 181 GSEYGQKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPPRVFRSISPAAKD 240

Query: 241 LMKKMICRDVSRRFSAEQALRHSWILSGGEGTSSVE 277
           LMKKMICRDVSRRFSAEQALRH WI+SGGEGTSSVE
Sbjct: 241 LMKKMICRDVSRRFSAEQALRHPWIMSGGEGTSSVE 276

BLAST of Chy2G040510 vs. NCBI nr
Match: XP_011660276.2 (LOW QUALITY PROTEIN: phosphoenolpyruvate carboxylase kinase 1 [Cucumis sativus])

HSP 1 Score: 556 bits (1434), Expect = 5.90e-200
Identity = 271/276 (98.19%), Postives = 272/276 (98.55%), Query Frame = 0

Query: 1   MADMIVNSQYQILEEIGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPK 60
           MADMIVNSQYQILEE+ RGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPK
Sbjct: 1   MADMIVNSQYQILEELXRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPK 60

Query: 61  IMALLPPHPNILQLIDVLENDHYLCLISDLCESLSLYDRIIRRPFSESEAAVVMKQLLQA 120
           IMALLP HPNILQLIDVLENDHYLCLISDLCESLSLYDRIIRRPFSESEAAVVMKQLLQA
Sbjct: 61  IMALLPSHPNILQLIDVLENDHYLCLISDLCESLSLYDRIIRRPFSESEAAVVMKQLLQA 120

Query: 121 LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLR 180
           LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLR
Sbjct: 121 LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLR 180

Query: 181 GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240
           GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD
Sbjct: 181 GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240

Query: 241 LMKKMICRDVSRRFSAEQALRHSWILSGGEGTSSVE 276
           LMKKMICRDVSRRFSAEQALRH WILSGGE TSSVE
Sbjct: 241 LMKKMICRDVSRRFSAEQALRHPWILSGGEATSSVE 276

BLAST of Chy2G040510 vs. NCBI nr
Match: XP_008462700.1 (PREDICTED: phosphoenolpyruvate carboxylase kinase 1 [Cucumis melo] >KAA0046511.1 phosphoenolpyruvate carboxylase kinase 1 [Cucumis melo var. makuwa] >TYK28749.1 phosphoenolpyruvate carboxylase kinase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 555 bits (1431), Expect = 1.69e-199
Identity = 271/276 (98.19%), Postives = 271/276 (98.19%), Query Frame = 0

Query: 1   MADMIVNSQYQILEEIGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPK 60
           MADMIVNSQYQILEEIGRGRFGIITRCFCPISNKFFACKTISK DL DETDKECLVKEPK
Sbjct: 1   MADMIVNSQYQILEEIGRGRFGIITRCFCPISNKFFACKTISKTDLIDETDKECLVKEPK 60

Query: 61  IMALLPPHPNILQLIDVLENDHYLCLISDLCESLSLYDRIIRRPFSESEAAVVMKQLLQA 120
           IMALLPPHPNILQLIDVLENDHYLCLISDLCE LSLYDRIIRRPFSESEAAVVMKQLLQA
Sbjct: 61  IMALLPPHPNILQLIDVLENDHYLCLISDLCEPLSLYDRIIRRPFSESEAAVVMKQLLQA 120

Query: 121 LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLR 180
           LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLR
Sbjct: 121 LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLR 180

Query: 181 GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240
           GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD
Sbjct: 181 GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240

Query: 241 LMKKMICRDVSRRFSAEQALRHSWILSGGEGTSSVE 276
           LMKKMICRDVSRR SAEQALRH WILSGGEGTSSVE
Sbjct: 241 LMKKMICRDVSRRLSAEQALRHPWILSGGEGTSSVE 276

BLAST of Chy2G040510 vs. NCBI nr
Match: XP_038877083.1 (phosphoenolpyruvate carboxylase kinase 1-like [Benincasa hispida])

HSP 1 Score: 536 bits (1382), Expect = 5.19e-192
Identity = 259/277 (93.50%), Postives = 270/277 (97.47%), Query Frame = 0

Query: 1   MADMIVNSQYQILEEIGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPK 60
           M DMIVNSQYQILEEIGRGRFG ITRCFCPISNKFFACKTI+KNDL DETDKECL KEPK
Sbjct: 1   MGDMIVNSQYQILEEIGRGRFGTITRCFCPISNKFFACKTIAKNDLIDETDKECLEKEPK 60

Query: 61  IMALLPPHPNILQLIDVLENDHYLCLISDLCESLSLYDRIIRRPFSESEAAVVMKQLLQA 120
           IMA+LPPHPNILQLIDVLEND YLCLISDLC+SLSLYDRIIRRPFSESEAA+V+KQLL+A
Sbjct: 61  IMAILPPHPNILQLIDVLENDDYLCLISDLCDSLSLYDRIIRRPFSESEAAIVIKQLLEA 120

Query: 121 LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLR 180
           LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSA+WCDEDGFM+GVVGTPYY+APEVLR
Sbjct: 121 LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSADWCDEDGFMFGVVGTPYYIAPEVLR 180

Query: 181 GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240
           GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD
Sbjct: 181 GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240

Query: 241 LMKKMICRDVSRRFSAEQALRHSWILSGGEGTS-SVE 276
           LMKKMICRDVSRRFSAEQ+LRH WILSGGEGT+ SVE
Sbjct: 241 LMKKMICRDVSRRFSAEQSLRHPWILSGGEGTATSVE 277

BLAST of Chy2G040510 vs. NCBI nr
Match: XP_022988181.1 (phosphoenolpyruvate carboxylase kinase 1-like [Cucurbita maxima])

HSP 1 Score: 527 bits (1358), Expect = 2.28e-188
Identity = 252/276 (91.30%), Postives = 266/276 (96.38%), Query Frame = 0

Query: 1   MADMIVNSQYQILEEIGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPK 60
           M DMIVNSQY+ILEEIGRGRFG ITRCFCPISNKFFACKTI+K+DL DETDKECL KEPK
Sbjct: 1   MGDMIVNSQYEILEEIGRGRFGTITRCFCPISNKFFACKTIAKSDLIDETDKECLEKEPK 60

Query: 61  IMALLPPHPNILQLIDVLENDHYLCLISDLCESLSLYDRIIRRPFSESEAAVVMKQLLQA 120
           IMALLPPHPNILQLIDV END YLCLIS+LC+S+SLYDRIIRRPFSESEAA+VMKQLLQA
Sbjct: 61  IMALLPPHPNILQLIDVFENDDYLCLISELCDSVSLYDRIIRRPFSESEAAIVMKQLLQA 120

Query: 121 LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLR 180
           LAHCH HGVVHRDVKPDNVLFDSR+NLKLIDFGSAEWCD+DGF +GVVGTPYY+APEVLR
Sbjct: 121 LAHCHYHGVVHRDVKPDNVLFDSRDNLKLIDFGSAEWCDKDGFTFGVVGTPYYIAPEVLR 180

Query: 181 GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240
           GSEYG+KVDVWSAGVILYTMLAGFPPFYG+SAAEVFEAVLRGNLRFP RVFRS+SSAAKD
Sbjct: 181 GSEYGQKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPPRVFRSISSAAKD 240

Query: 241 LMKKMICRDVSRRFSAEQALRHSWILSGGEGTSSVE 276
           LMKKMICRDVSRRFSAEQALRH WILSGGEGTSSVE
Sbjct: 241 LMKKMICRDVSRRFSAEQALRHPWILSGGEGTSSVE 276

BLAST of Chy2G040510 vs. NCBI nr
Match: XP_022961225.1 (phosphoenolpyruvate carboxylase kinase 1-like [Cucurbita moschata] >XP_022961226.1 phosphoenolpyruvate carboxylase kinase 1-like [Cucurbita moschata])

HSP 1 Score: 525 bits (1351), Expect = 2.66e-187
Identity = 250/276 (90.58%), Postives = 265/276 (96.01%), Query Frame = 0

Query: 1   MADMIVNSQYQILEEIGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPK 60
           M DMIVNSQY+ILEEIGRGRFG ITRCFCPISNKFFACKTI+K+DL DETDKECL KEPK
Sbjct: 1   MGDMIVNSQYEILEEIGRGRFGTITRCFCPISNKFFACKTIAKSDLIDETDKECLEKEPK 60

Query: 61  IMALLPPHPNILQLIDVLENDHYLCLISDLCESLSLYDRIIRRPFSESEAAVVMKQLLQA 120
           IMALLPPHPNILQLIDV END YLCLIS+LC+S+SLYDRIIRRPFSESEAA+VMKQLLQA
Sbjct: 61  IMALLPPHPNILQLIDVFENDDYLCLISELCDSVSLYDRIIRRPFSESEAAIVMKQLLQA 120

Query: 121 LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLR 180
           LAHCH HGVVHRDVKPDNVLFDSR+NLKLIDFGSAEWCD+DGF +GVVGTPYY+APEVLR
Sbjct: 121 LAHCHYHGVVHRDVKPDNVLFDSRDNLKLIDFGSAEWCDKDGFTFGVVGTPYYIAPEVLR 180

Query: 181 GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240
           GSEYG+KVDVWSAGVILYTMLAGFPPFYG+SAAEVFEAVLRGNLRFP RVFRS+S AAKD
Sbjct: 181 GSEYGQKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPPRVFRSISPAAKD 240

Query: 241 LMKKMICRDVSRRFSAEQALRHSWILSGGEGTSSVE 276
           LMKKMICRDVSRRFSAEQALRH WI+SGGEGTSSVE
Sbjct: 241 LMKKMICRDVSRRFSAEQALRHPWIMSGGEGTSSVE 276

BLAST of Chy2G040510 vs. TAIR 10
Match: AT1G08650.1 (phosphoenolpyruvate carboxylase kinase 1 )

HSP 1 Score: 336.7 bits (862), Expect = 1.8e-92
Identity = 161/266 (60.53%), Postives = 202/266 (75.94%), Query Frame = 0

Query: 8   SQYQILEEIGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPKIMALLPP 67
           ++YQI EEIGRGRFG ++R + P +  FFACKTI K  L+D+ D+ CL  EPK+MALL  
Sbjct: 13  NKYQICEEIGRGRFGTVSRVYAPATGDFFACKTIDKASLSDDLDRACLDNEPKLMALLSY 72

Query: 68  HPNILQLIDVLENDHYLCLISDLCE-SLSLYDRIIRR-PFSESEAAVVMKQLLQALAHCH 127
           HPNI+Q+ D+++ D  L +  +L   S+S+YDR++    F E + A   KQ+LQAL+HCH
Sbjct: 73  HPNIVQIHDLIDTDSTLSIFMELVHPSVSIYDRLVSSGTFFEPQTASFAKQILQALSHCH 132

Query: 128 SHGVVHRDVKPDNVLFDSRNN-LKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLRGSEY 187
            +GVVHRD+KP+N+L D RN+ +K+ DFGS  W  E     GVVGTPYYVAPEVL G  Y
Sbjct: 133 RYGVVHRDIKPENILVDLRNDTVKICDFGSGIWLGEGETTEGVVGTPYYVAPEVLMGYSY 192

Query: 188 GRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKDLMKK 247
           G KVD+WSAGV+LYTMLAG PPFYGE+A E+FEAVLRGNLRFPT++FR VSS AKD ++K
Sbjct: 193 GEKVDLWSAGVVLYTMLAGTPPFYGETAEEIFEAVLRGNLRFPTKIFRGVSSMAKDFLRK 252

Query: 248 MICRDVSRRFSAEQALRHSWILSGGE 271
           +IC+D SRRFSAEQALRH WI   GE
Sbjct: 253 LICKDASRRFSAEQALRHPWIQRAGE 278

BLAST of Chy2G040510 vs. TAIR 10
Match: AT3G04530.1 (phosphoenolpyruvate carboxylase kinase 2 )

HSP 1 Score: 324.3 bits (830), Expect = 9.4e-89
Identity = 158/268 (58.96%), Postives = 202/268 (75.37%), Query Frame = 0

Query: 6   VNSQYQILEEIGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPKIMALL 65
           + + YQ+ +EIGRGRFG ITRCF P + +F+ACKTI K  L D  D+EC+  EP+IMA+L
Sbjct: 7   LENNYQLCDEIGRGRFGTITRCFSPATKEFYACKTIDKRVLIDALDRECIETEPRIMAML 66

Query: 66  PPHPNILQLIDVLENDHYLCLISDLCE-SLSLYDRIIRR--PFSESEAAVVMKQLLQALA 125
           PPHPNI+++ D+ E +  L ++ +L +  +++YDR+I      SESE+A   KQ+L ALA
Sbjct: 67  PPHPNIIRIFDLYETEDSLAIVMELVDPPMTIYDRLISAGGRLSESESASYAKQILSALA 126

Query: 126 HCHSHGVVHRDVKPDNVLFD-SRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLRG 185
           HCH   VVHRDVKPDNVL D     +KL DFGSA W   +    GVVGTPYYVAPEV+ G
Sbjct: 127 HCHRCDVVHRDVKPDNVLVDLVSGGVKLCDFGSAVWLGGE-TAEGVVGTPYYVAPEVVMG 186

Query: 186 SEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKDL 245
            +Y  KVD+WSAGV++YTMLAG PPF GE+A ++FE++LRGNLRFP + F SVSS AKDL
Sbjct: 187 RKYDEKVDIWSAGVVIYTMLAGEPPFNGETAEDIFESILRGNLRFPPKKFGSVSSEAKDL 246

Query: 246 MKKMICRDVSRRFSAEQALRHSWILSGG 270
           ++KMICRDVSRRFSAE ALRHSW+++ G
Sbjct: 247 LRKMICRDVSRRFSAEDALRHSWMMNVG 273

BLAST of Chy2G040510 vs. TAIR 10
Match: AT1G08650.2 (phosphoenolpyruvate carboxylase kinase 1 )

HSP 1 Score: 323.9 bits (829), Expect = 1.2e-88
Identity = 157/260 (60.38%), Postives = 198/260 (76.15%), Query Frame = 0

Query: 8   SQYQILEEIGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPKIMALLPP 67
           ++YQI EEIGRGRFG ++R + P +  FFACKTI K  L+D+ D+ CL  EPK+MALL  
Sbjct: 13  NKYQICEEIGRGRFGTVSRVYAPATGDFFACKTIDKASLSDDLDRACLDNEPKLMALLSY 72

Query: 68  HPNILQLIDVLENDHYLCLISDLCE-SLSLYDRIIRR-PFSESEAAVVMKQLLQALAHCH 127
           HPNI+Q+ D+++ D  L +  +L   S+S+YDR++    F E + A   KQ+LQAL+HCH
Sbjct: 73  HPNIVQIHDLIDTDSTLSIFMELVHPSVSIYDRLVSSGTFFEPQTASFAKQILQALSHCH 132

Query: 128 SHGVVHRDVKPDNVLFDSRNN-LKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLRGSEY 187
            +GVVHRD+KP+N+L D RN+ +K+ DFGS  W  E     GVVGTPYYVAPEVL G  Y
Sbjct: 133 RYGVVHRDIKPENILVDLRNDTVKICDFGSGIWLGEGETTEGVVGTPYYVAPEVLMGYSY 192

Query: 188 GRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKDLMKK 247
           G KVD+WSAGV+LYTMLAG PPFYGE+A E+FEAVLRGNLRFPT++FR VSS AKD ++K
Sbjct: 193 GEKVDLWSAGVVLYTMLAGTPPFYGETAEEIFEAVLRGNLRFPTKIFRGVSSMAKDFLRK 252

Query: 248 MICRDVSRRFSAEQALRHSW 265
           +IC+D SRRFSAEQAL  SW
Sbjct: 253 LICKDASRRFSAEQAL--SW 270

BLAST of Chy2G040510 vs. TAIR 10
Match: AT5G19450.1 (calcium-dependent protein kinase 19 )

HSP 1 Score: 220.7 bits (561), Expect = 1.5e-57
Identity = 115/264 (43.56%), Postives = 164/264 (62.12%), Query Frame = 0

Query: 6   VNSQYQILEEIGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPKIMALL 65
           ++  Y +  E+GRG FGI   C    + + +ACK+ISK  L    D E + +E +IM  +
Sbjct: 53  ISLMYDLGREVGRGEFGITYLCTDIKTGEKYACKSISKKKLRTAVDIEDVRREVEIMKHM 112

Query: 66  PPHPNILQLIDVLENDHYLCLISDLCESLSLYDRIIRR-PFSESEAAVVMKQLLQALAHC 125
           P HPNI+ L D  E+D  + ++ +LCE   L+DRI+ R  ++E  AA VMK +L+ +  C
Sbjct: 113 PRHPNIVSLKDAFEDDDAVHIVMELCEGGELFDRIVARGHYTERAAAAVMKTILEVVQIC 172

Query: 126 HSHGVVHRDVKPDNVLFDSRNN---LKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLRG 185
           H HGV+HRD+KP+N LF ++     LK IDFG + +         +VG+PYY+APEVLR 
Sbjct: 173 HKHGVMHRDLKPENFLFANKKETSALKAIDFGLSVFFKPGEGFNEIVGSPYYMAPEVLR- 232

Query: 186 SEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKDL 245
             YG +VD+WSAGVILY +L G PPF+ E+   V +A++R  + F    +  VS  AKDL
Sbjct: 233 RNYGPEVDIWSAGVILYILLCGVPPFWAETEQGVAQAIIRSVIDFKRDPWPRVSETAKDL 292

Query: 246 MKKMICRDVSRRFSAEQALRHSWI 266
           ++KM+  D  +R SA Q L HSWI
Sbjct: 293 VRKMLEPDPKKRLSAAQVLEHSWI 315

BLAST of Chy2G040510 vs. TAIR 10
Match: AT5G19450.2 (calcium-dependent protein kinase 19 )

HSP 1 Score: 220.7 bits (561), Expect = 1.5e-57
Identity = 115/264 (43.56%), Postives = 164/264 (62.12%), Query Frame = 0

Query: 6   VNSQYQILEEIGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPKIMALL 65
           ++  Y +  E+GRG FGI   C    + + +ACK+ISK  L    D E + +E +IM  +
Sbjct: 53  ISLMYDLGREVGRGEFGITYLCTDIKTGEKYACKSISKKKLRTAVDIEDVRREVEIMKHM 112

Query: 66  PPHPNILQLIDVLENDHYLCLISDLCESLSLYDRIIRR-PFSESEAAVVMKQLLQALAHC 125
           P HPNI+ L D  E+D  + ++ +LCE   L+DRI+ R  ++E  AA VMK +L+ +  C
Sbjct: 113 PRHPNIVSLKDAFEDDDAVHIVMELCEGGELFDRIVARGHYTERAAAAVMKTILEVVQIC 172

Query: 126 HSHGVVHRDVKPDNVLFDSRNN---LKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLRG 185
           H HGV+HRD+KP+N LF ++     LK IDFG + +         +VG+PYY+APEVLR 
Sbjct: 173 HKHGVMHRDLKPENFLFANKKETSALKAIDFGLSVFFKPGEGFNEIVGSPYYMAPEVLR- 232

Query: 186 SEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKDL 245
             YG +VD+WSAGVILY +L G PPF+ E+   V +A++R  + F    +  VS  AKDL
Sbjct: 233 RNYGPEVDIWSAGVILYILLCGVPPFWAETEQGVAQAIIRSVIDFKRDPWPRVSETAKDL 292

Query: 246 MKKMICRDVSRRFSAEQALRHSWI 266
           ++KM+  D  +R SA Q L HSWI
Sbjct: 293 VRKMLEPDPKKRLSAAQVLEHSWI 315

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SPK42.6e-9160.53Phosphoenolpyruvate carboxylase kinase 1 OS=Arabidopsis thaliana OX=3702 GN=PPCK... [more]
Q93VK01.3e-8758.96Phosphoenolpyruvate carboxylase kinase 2 OS=Arabidopsis thaliana OX=3702 GN=PPCK... [more]
Q424382.1e-5643.56Calcium-dependent protein kinase 8 OS=Arabidopsis thaliana OX=3702 GN=CPK8 PE=1 ... [more]
P937594.6e-5642.32Calcium-dependent protein kinase 14 OS=Arabidopsis thaliana OX=3702 GN=CPK14 PE=... [more]
P285824.6e-5640.59Calcium-dependent protein kinase OS=Daucus carota OX=4039 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A0A0M3G71.2e-15598.55Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G690... [more]
A0A5A7TYV21.7e-15498.19Phosphoenolpyruvate carboxylase kinase 1 OS=Cucumis melo var. makuwa OX=1194695 ... [more]
A0A1S3CHK91.7e-15498.19phosphoenolpyruvate carboxylase kinase 1 OS=Cucumis melo OX=3656 GN=LOC103501000... [more]
A0A6J1JGI45.0e-14691.30phosphoenolpyruvate carboxylase kinase 1-like OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A6J1HBL33.2e-14590.58phosphoenolpyruvate carboxylase kinase 1-like OS=Cucurbita moschata OX=3662 GN=L... [more]
Match NameE-valueIdentityDescription
XP_011660276.25.90e-20098.19LOW QUALITY PROTEIN: phosphoenolpyruvate carboxylase kinase 1 [Cucumis sativus][more]
XP_008462700.11.69e-19998.19PREDICTED: phosphoenolpyruvate carboxylase kinase 1 [Cucumis melo] >KAA0046511.1... [more]
XP_038877083.15.19e-19293.50phosphoenolpyruvate carboxylase kinase 1-like [Benincasa hispida][more]
XP_022988181.12.28e-18891.30phosphoenolpyruvate carboxylase kinase 1-like [Cucurbita maxima][more]
XP_022961225.12.66e-18790.58phosphoenolpyruvate carboxylase kinase 1-like [Cucurbita moschata] >XP_022961226... [more]
Match NameE-valueIdentityDescription
AT1G08650.11.8e-9260.53phosphoenolpyruvate carboxylase kinase 1 [more]
AT3G04530.19.4e-8958.96phosphoenolpyruvate carboxylase kinase 2 [more]
AT1G08650.21.2e-8860.38phosphoenolpyruvate carboxylase kinase 1 [more]
AT5G19450.11.5e-5743.56calcium-dependent protein kinase 19 [more]
AT5G19450.21.5e-5743.56calcium-dependent protein kinase 19 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 10..265
e-value: 1.7E-91
score: 319.9
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 10..265
e-value: 9.9E-72
score: 241.5
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 10..265
score: 47.523468
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 3..275
e-value: 2.9E-87
score: 295.0
NoneNo IPR availablePANTHERPTHR24349SERINE/THREONINE-PROTEIN KINASEcoord: 6..274
NoneNo IPR availablePANTHERPTHR24349:SF410PHOSPHOENOLPYRUVATE CARBOXYLASE KINASE 1coord: 6..274
NoneNo IPR availableCDDcd05117STKc_CAMKcoord: 9..264
e-value: 1.71584E-122
score: 348.312
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 129..141
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 16..39
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 4..267

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy2G040510.1Chy2G040510.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0035556 intracellular signal transduction
biological_process GO:0018105 peptidyl-serine phosphorylation
biological_process GO:0046777 protein autophosphorylation
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0005524 ATP binding
molecular_function GO:0009931 calcium-dependent protein serine/threonine kinase activity
molecular_function GO:0005516 calmodulin binding
molecular_function GO:0004683 calmodulin-dependent protein kinase activity
molecular_function GO:0004672 protein kinase activity