Chy2G040170 (gene) Cucumber (hystrix) v1

Overview


Name	Chy2G040170
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	Proteinase inhibitor-like
Location	chrH02: 18473573 .. 18473952 (+)
RNA-Seq Expression	Chy2G040170
Synteny	Chy2G040170

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTGGAGTTTCTTGGCCTCCATACCCTCCATGTGTTAGTGGTACTTGTACAGATTCTGCCTGTTGTACTTTAGGTCGAGGTAAGATTTTGTTTTATATTTTCAAATAACATTTACTAGAAGGGACAGTTTAGTTTAGTTTATTTTATAAATGCTAAAAAGGTGGTTAATTAATACAGGACAACGTAAGTTTAGATGGCCAGAACTAATGGGAACAAATGGAGGAGAAGCCAAAAAGCAAATAAACAATGACGCCCCTTTTGTGACAGTTGTTTTTATTAAACCGGGGCAGGTTTCACTACCTAATTTTTGCTGCAATCGTGTTAATGTCGTGCTTGATCCAACTGGAAAGGTCTCCAATATTCCATTCATTGGTTAA

mRNA sequence

ATGTCTGGAGTTTCTTGGCCTCCATACCCTCCATGTGTTAGTGGTACTTGTACAGATTCTGCCTGTTGTACTTTAGGTCGAGGACAACGTAAGTTTAGATGGCCAGAACTAATGGGAACAAATGGAGGAGAAGCCAAAAAGCAAATAAACAATGACGCCCCTTTTGTGACAGTTGTTTTTATTAAACCGGGGCAGGTTTCACTACCTAATTTTTGCTGCAATCGTGTTAATGTCGTGCTTGATCCAACTGGAAAGGTCTCCAATATTCCATTCATTGGTTAA

Coding sequence (CDS)

ATGTCTGGAGTTTCTTGGCCTCCATACCCTCCATGTGTTAGTGGTACTTGTACAGATTCTGCCTGTTGTACTTTAGGTCGAGGACAACGTAAGTTTAGATGGCCAGAACTAATGGGAACAAATGGAGGAGAAGCCAAAAAGCAAATAAACAATGACGCCCCTTTTGTGACAGTTGTTTTTATTAAACCGGGGCAGGTTTCACTACCTAATTTTTGCTGCAATCGTGTTAATGTCGTGCTTGATCCAACTGGAAAGGTCTCCAATATTCCATTCATTGGTTAA

Protein sequence

MSGVSWPPYPPCVSGTCTDSACCTLGRGQRKFRWPELMGTNGGEAKKQINNDAPFVTVVFIKPGQVSLPNFCCNRVNVVLDPTGKVSNIPFIG*

Homology

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BLAST of Chy2G040170 vs. ExPASy Swiss-Prot
Match: P24076 (Glu S.griseus protease inhibitor OS=Momordica charantia OX=3673 PE=1 SV=1)

HSP 1 Score: 49.3 bits (116), Expect = 2.8e-05
Identity = 24/65 (36.92%), Postives = 34/65 (52.31%), Query Frame = 0


Query: 29 QRKFRWPELMGTNGGEAKKQINNDAPFVTVVFIKPGQVSLPNFCCNRVNVVLDPTGKVSN 88
          Q K  WP+L+G+ G  AK  I  + P V  V ++ G     +F C+RV V +   G V+ 
Sbjct: 4  QGKRSWPQLVGSTGAAAKAVIERENPRVRAVIVRVGSPVTADFRCDRVRVWVTERGIVAR 63

Query: 89 IPFIG 94
           P IG
Sbjct: 64 PPAIG 68

[Close]

BLAST of Chy2G040170 vs. ExPASy Swiss-Prot
Match: P82381 (Proteinase inhibitor OS=Linum usitatissimum OX=4006 PE=1 SV=1)

HSP 1 Score: 45.8 bits (107), Expect = 3.1e-04
Identity = 20/62 (32.26%), Postives = 34/62 (54.84%), Query Frame = 0


Query: 31 KFRWPELMGTNGGEAKKQINNDAPFVTVVFIKPGQVSLPNFCCNRVNVVLDPTGKVSNIP 90
          K  WPEL+G +G  A   +  +   V  + +K G     +F C+RV V+++  G V+++P
Sbjct: 7  KNAWPELVGKSGNMAAATVERENRNVHAIVLKEGSAMTKDFRCDRVWVIVNDHGVVTSVP 66

Query: 91 FI 93
           I
Sbjct: 67 HI 68

[Close]

BLAST of Chy2G040170 vs. ExPASy TrEMBL
Match: A0A5D3DF00 (Proteinase inhibitor-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold543G00060 PE=3 SV=1)

HSP 1 Score: 194.5 bits (493), Expect = 2.0e-46
Identity = 85/93 (91.40%), Postives = 89/93 (95.70%), Query Frame = 0


Query: 1  MSGVSWPPYPPCVSGTCTDSACCTLGRGQRKFRWPELMGTNGGEAKKQINNDAPFVTVVF 60
          MSGVSWPPYPPCVSGTCTDSACCTLGRGQRKFRWPEL G NGG+AK QIN DAPFVTVVF
Sbjct: 1  MSGVSWPPYPPCVSGTCTDSACCTLGRGQRKFRWPELRGKNGGDAKNQINKDAPFVTVVF 60

Query: 61 IKPGQVSLPNFCCNRVNVVLDPTGKVSNIPFIG 94
          I+PGQV+LPNFCCNRVNVVLDP+GKVSNIPFIG
Sbjct: 61 IRPGQVALPNFCCNRVNVVLDPSGKVSNIPFIG 93

[Close]

BLAST of Chy2G040170 vs. ExPASy TrEMBL
Match: A0A5A7SX46 (Proteinase inhibitor-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold57G002800 PE=3 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 9.8e-46
Identity = 84/93 (90.32%), Postives = 88/93 (94.62%), Query Frame = 0


Query: 1  MSGVSWPPYPPCVSGTCTDSACCTLGRGQRKFRWPELMGTNGGEAKKQINNDAPFVTVVF 60
          MSGVSWPPYPPCVSGTCTDSACCTLGRGQRKFRWPEL G NG +AK QIN DAPFVTVVF
Sbjct: 1  MSGVSWPPYPPCVSGTCTDSACCTLGRGQRKFRWPELRGKNGADAKNQINKDAPFVTVVF 60

Query: 61 IKPGQVSLPNFCCNRVNVVLDPTGKVSNIPFIG 94
          I+PGQV+LPNFCCNRVNVVLDP+GKVSNIPFIG
Sbjct: 61 IRPGQVALPNFCCNRVNVVLDPSGKVSNIPFIG 93

[Close]

BLAST of Chy2G040170 vs. ExPASy TrEMBL
Match: A0A5A7SZ39 (UPF0261 protein y4oU OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold57G002880 PE=3 SV=1)

HSP 1 Score: 177.2 bits (448), Expect = 3.3e-41
Identity = 77/86 (89.53%), Postives = 81/86 (94.19%), Query Frame = 0


Query: 1  MSGVSWPPYPPCVSGTCTDSACCTLGRGQRKFRWPELMGTNGGEAKKQINNDAPFVTVVF 60
          MSGVSWPPYPPCVSGTCTDSACCTLGRGQRKFRWPEL G NG +AK QIN DAPFVTVVF
Sbjct: 1  MSGVSWPPYPPCVSGTCTDSACCTLGRGQRKFRWPELRGKNGADAKNQINKDAPFVTVVF 60

Query: 61 IKPGQVSLPNFCCNRVNVVLDPTGKV 87
          I+PGQV+LPNFCCNRVNVVLDP+GKV
Sbjct: 61 IRPGQVALPNFCCNRVNVVLDPSGKV 86

[Close]

BLAST of Chy2G040170 vs. ExPASy TrEMBL
Match: A0A6P6AIT5 (proteinase inhibitor-like OS=Durio zibethinus OX=66656 GN=LOC111309978 PE=3 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 2.4e-23
Identity = 53/93 (56.99%), Postives = 63/93 (67.74%), Query Frame = 0


Query: 1  MSGVSWPPYPPCVSGTCTDSACCTLGRGQRKFRWPELMGTNGGEAKKQINNDAPFVTVVF 60
          MS  +WP YPPC SG+CTD  CC+ G   RKF WPEL+G NG  AK  I  D P VTV  
Sbjct: 1  MSQGTWPRYPPCASGSCTDPICCSHG---RKFTWPELLGKNGQVAKATIEKDNPEVTVEI 60

Query: 61 IKPGQVSLPNFCCNRVNVVLDPTGKVSNIPFIG 94
          I PG+V  P+FCCNRV +VLD  G V+N+P +G
Sbjct: 61 ITPGRVGFPDFCCNRVYLVLDNNGNVTNMPTVG 90

[Close]

BLAST of Chy2G040170 vs. ExPASy TrEMBL
Match: A0A0D2TFV2 (Uncharacterized protein OS=Gossypium raimondii OX=29730 GN=B456_009G297200 PE=3 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 2.0e-22
Identity = 53/93 (56.99%), Postives = 60/93 (64.52%), Query Frame = 0


Query: 1  MSGVSWPPYPPCVSGTCTDSACCTLGRGQRKFRWPELMGTNGGEAKKQINNDAPFVTVVF 60
          MSG  WP YPPC SGTCTD  CC  G    K RWPEL+G NG  AK  I  + P VT   
Sbjct: 1  MSG--WPRYPPCASGTCTDVVCCAHG---HKLRWPELLGENGAAAKATIEKENPEVTAEI 60

Query: 61 IKPGQVSLPNFCCNRVNVVLDPTGKVSNIPFIG 94
          + PG+V  PNFCCNRV V++D  G V+NIP IG
Sbjct: 61 VTPGRVGPPNFCCNRVFVIVDTHGNVTNIPTIG 88

[Close]

BLAST of Chy2G040170 vs. NCBI nr
Match: TYK07514.1 (proteinase inhibitor-like [Cucumis melo var. makuwa] >TYK21960.1 proteinase inhibitor-like [Cucumis melo var. makuwa] >TYK21972.1 proteinase inhibitor-like [Cucumis melo var. makuwa])

HSP 1 Score: 194 bits (493), Expect = 1.75e-62
Identity = 85/93 (91.40%), Postives = 89/93 (95.70%), Query Frame = 0


Query: 1  MSGVSWPPYPPCVSGTCTDSACCTLGRGQRKFRWPELMGTNGGEAKKQINNDAPFVTVVF 60
          MSGVSWPPYPPCVSGTCTDSACCTLGRGQRKFRWPEL G NGG+AK QIN DAPFVTVVF
Sbjct: 1  MSGVSWPPYPPCVSGTCTDSACCTLGRGQRKFRWPELRGKNGGDAKNQINKDAPFVTVVF 60

Query: 61 IKPGQVSLPNFCCNRVNVVLDPTGKVSNIPFIG 93
          I+PGQV+LPNFCCNRVNVVLDP+GKVSNIPFIG
Sbjct: 61 IRPGQVALPNFCCNRVNVVLDPSGKVSNIPFIG 93

[Close]

BLAST of Chy2G040170 vs. NCBI nr
Match: KAA0035178.1 (proteinase inhibitor-like [Cucumis melo var. makuwa])

HSP 1 Score: 192 bits (487), Expect = 1.44e-61
Identity = 84/93 (90.32%), Postives = 88/93 (94.62%), Query Frame = 0


Query: 1  MSGVSWPPYPPCVSGTCTDSACCTLGRGQRKFRWPELMGTNGGEAKKQINNDAPFVTVVF 60
          MSGVSWPPYPPCVSGTCTDSACCTLGRGQRKFRWPEL G NG +AK QIN DAPFVTVVF
Sbjct: 1  MSGVSWPPYPPCVSGTCTDSACCTLGRGQRKFRWPELRGKNGADAKNQINKDAPFVTVVF 60

Query: 61 IKPGQVSLPNFCCNRVNVVLDPTGKVSNIPFIG 93
          I+PGQV+LPNFCCNRVNVVLDP+GKVSNIPFIG
Sbjct: 61 IRPGQVALPNFCCNRVNVVLDPSGKVSNIPFIG 93

[Close]

BLAST of Chy2G040170 vs. NCBI nr
Match: KAA0035186.1 (UPF0261 protein y4oU [Cucumis melo var. makuwa])

HSP 1 Score: 177 bits (449), Expect = 1.22e-54
Identity = 77/86 (89.53%), Postives = 81/86 (94.19%), Query Frame = 0


Query: 1  MSGVSWPPYPPCVSGTCTDSACCTLGRGQRKFRWPELMGTNGGEAKKQINNDAPFVTVVF 60
          MSGVSWPPYPPCVSGTCTDSACCTLGRGQRKFRWPEL G NG +AK QIN DAPFVTVVF
Sbjct: 1  MSGVSWPPYPPCVSGTCTDSACCTLGRGQRKFRWPELRGKNGADAKNQINKDAPFVTVVF 60

Query: 61 IKPGQVSLPNFCCNRVNVVLDPTGKV 86
          I+PGQV+LPNFCCNRVNVVLDP+GKV
Sbjct: 61 IRPGQVALPNFCCNRVNVVLDPSGKV 86

[Close]

BLAST of Chy2G040170 vs. NCBI nr
Match: XP_022764760.1 (proteinase inhibitor-like [Durio zibethinus])

HSP 1 Score: 117 bits (294), Expect = 3.58e-32
Identity = 53/93 (56.99%), Postives = 63/93 (67.74%), Query Frame = 0


Query: 1  MSGVSWPPYPPCVSGTCTDSACCTLGRGQRKFRWPELMGTNGGEAKKQINNDAPFVTVVF 60
          MS  +WP YPPC SG+CTD  CC+ GR   KF WPEL+G NG  AK  I  D P VTV  
Sbjct: 1  MSQGTWPRYPPCASGSCTDPICCSHGR---KFTWPELLGKNGQVAKATIEKDNPEVTVEI 60

Query: 61 IKPGQVSLPNFCCNRVNVVLDPTGKVSNIPFIG 93
          I PG+V  P+FCCNRV +VLD  G V+N+P +G
Sbjct: 61 ITPGRVGFPDFCCNRVYLVLDNNGNVTNMPTVG 90

[Close]

BLAST of Chy2G040170 vs. NCBI nr
Match: XP_012442756.1 (PREDICTED: glu S.griseus protease inhibitor-like [Gossypium raimondii] >KAB2031338.1 hypothetical protein ES319_D05G298100v1 [Gossypium barbadense] >KJB53436.1 hypothetical protein B456_009G297200 [Gossypium raimondii] >MBA0775872.1 hypothetical protein [Gossypium trilobum])

HSP 1 Score: 114 bits (286), Expect = 5.60e-31
Identity = 53/93 (56.99%), Postives = 60/93 (64.52%), Query Frame = 0


Query: 1  MSGVSWPPYPPCVSGTCTDSACCTLGRGQRKFRWPELMGTNGGEAKKQINNDAPFVTVVF 60
          MSG  WP YPPC SGTCTD  CC  G    K RWPEL+G NG  AK  I  + P VT   
Sbjct: 1  MSG--WPRYPPCASGTCTDVVCCAHGH---KLRWPELLGENGAAAKATIEKENPEVTAEI 60

Query: 61 IKPGQVSLPNFCCNRVNVVLDPTGKVSNIPFIG 93
          + PG+V  PNFCCNRV V++D  G V+NIP IG
Sbjct: 61 VTPGRVGPPNFCCNRVFVIVDTHGNVTNIPTIG 88

[Close]

BLAST of Chy2G040170 vs. TAIR 10
Match: AT3G46860.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 92.4 bits (228), Expect = 2.0e-19
Identity = 40/85 (47.06%), Postives = 54/85 (63.53%), Query Frame = 0


Query: 9  YPPCVSGTCTDSACCTLGRGQRKFRWPELMGTNGGEAKKQINNDAPFVTVVFIKPGQVSL 68
          YPPC SG+C D  CC +G   +K+RWPEL+G NG  AK  I  + P V  + ++ G+  +
Sbjct: 4  YPPCWSGSCEDPECCAIG---KKYRWPELVGKNGQLAKMTIERENPNVLAIVLRYGEKRI 63

Query: 69 PNFCCNRVNVVLDPTGKVSNIPFIG 94
           NFCCNRV V L   G+V++ P IG
Sbjct: 64 ENFCCNRVFVYLGSNGQVADAPMIG 85

[Close]

BLAST of Chy2G040170 vs. TAIR 10
Match: AT3G50020.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 62.8 bits (151), Expect = 1.7e-10
Identity = 39/88 (44.32%), Postives = 43/88 (48.86%), Query Frame = 0


Query: 10 PPCVSGTCTDSACCTLGRGQRKFRWPELMGTNGGEAKKQINNDAPFVTVVFIKPGQVSLP 69
          PPC   +C    C  L  G  K  WPEL G  G EAK+ I +D P V VV I      L 
Sbjct: 10 PPCQRCSCAGITCQPLFPG-IKVDWPELNGVKGLEAKRIIEHDNPKVVVVIIPDDVAVLA 69

Query: 70 NFCCNRVNVVLD----PTGKVSNIPFIG 94
            CCNRV V +     P G VSN P IG
Sbjct: 70 VNCCNRVIVRVPVNNCPNGPVSNFPHIG 96

[Close]

BLAST of Chy2G040170 vs. TAIR 10
Match: AT5G43580.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 51.6 bits (122), Expect = 4.0e-07
Identity = 26/63 (41.27%), Postives = 34/63 (53.97%), Query Frame = 0


Query: 31 KFRWPELMGTNGGEAKKQINNDAPFVTVVFIKPGQVSLPNFCCNRVNVVLDPTGKVSNIP 90
          K  WPEL+G  G +AK+ I  + P +  V I  G V    F C+RV V ++  G V  IP
Sbjct: 37 KSSWPELLGAKGEDAKEVIERENPKMKAVIILDGTVVPEIFICSRVYVWVNDCGIVVQIP 96

Query: 91 FIG 94
           IG
Sbjct: 97 IIG 99

[Close]

BLAST of Chy2G040170 vs. TAIR 10
Match: AT5G43570.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 43.5 bits (101), Expect = 1.1e-04
Identity = 25/67 (37.31%), Postives = 33/67 (49.25%), Query Frame = 0


Query: 24 TLGRGQRKFRWPELMGTNGGEAKKQINNDAPFVTVVFIKPGQVSLPNFCCNRVNVVLDPT 83
          T G G  K  WPEL+G  G E K+ I+ +   VT   I    V L    C+RV V ++  
Sbjct: 8  TAGEGM-KSSWPELVGRRGEEVKEIIDRENTKVTAKIISENAVVLAVVICDRVYVRVNDQ 67

Query: 84 GKVSNIP 91
          G V+  P
Sbjct: 68 GIVTRTP 73

The following BLAST results are available for this feature:

BLAST of Chy2G040170 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P24076	2.8e-05	36.92	Glu S.griseus protease inhibitor OS=Momordica charantia OX=3673 PE=1 SV=1	[more]
P82381	3.1e-04	32.26	Proteinase inhibitor OS=Linum usitatissimum OX=4006 PE=1 SV=1	[more]

BLAST of Chy2G040170 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3DF00	2.0e-46	91.40	Proteinase inhibitor-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]
A0A5A7SX46	9.8e-46	90.32	Proteinase inhibitor-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffo...	[more]
A0A5A7SZ39	3.3e-41	89.53	UPF0261 protein y4oU OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold57G...	[more]
A0A6P6AIT5	2.4e-23	56.99	proteinase inhibitor-like OS=Durio zibethinus OX=66656 GN=LOC111309978 PE=3 SV=1	[more]
A0A0D2TFV2	2.0e-22	56.99	Uncharacterized protein OS=Gossypium raimondii OX=29730 GN=B456_009G297200 PE=3 ...	[more]

BLAST of Chy2G040170 vs. NCBI nr


Match Name	E-value	Identity	Description
TYK07514.1	1.75e-62	91.40	proteinase inhibitor-like [Cucumis melo var. makuwa] >TYK21960.1 proteinase inhi...	[more]
KAA0035178.1	1.44e-61	90.32	proteinase inhibitor-like [Cucumis melo var. makuwa]	[more]
KAA0035186.1	1.22e-54	89.53	UPF0261 protein y4oU [Cucumis melo var. makuwa]	[more]
XP_022764760.1	3.58e-32	56.99	proteinase inhibitor-like [Durio zibethinus]	[more]
XP_012442756.1	5.60e-31	56.99	PREDICTED: glu S.griseus protease inhibitor-like [Gossypium raimondii] >KAB20313...	[more]

BLAST of Chy2G040170 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G46860.1	2.0e-19	47.06	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT3G50020.1	1.7e-10	44.32	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT5G43580.1	4.0e-07	41.27	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT5G43570.1	1.1e-04	37.31	Serine protease inhibitor, potato inhibitor I-type family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PFAM	PF00280	potato_inhibit	coord: 31..93 e-value: 1.4E-19 score: 70.5
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PANTHER	PTHR33091	PROTEIN, PUTATIVE, EXPRESSED-RELATED	coord: 9..93
None	No IPR available	GENE3D	3.30.10.10	Trypsin Inhibitor V, subunit A	coord: 24..93 e-value: 1.6E-20 score: 75.0
None	No IPR available	PANTHER	PTHR33091:SF44	SERINE PROTEASE INHIBITOR POTATO INHIBITOR I-TYPE FAMILY PROTEIN	coord: 9..93
IPR036354	Proteinase inhibitor I13, potato inhibitor I superfamily	SUPERFAMILY	54654	CI-2 family of serine protease inhibitors	coord: 29..93

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy2G040170.1	Chy2G040170.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
biological_process	GO:0009611	response to wounding
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity