Chy2G039530 (gene) Cucumber (hystrix) v1

Overview
NameChy2G039530
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionTryptophan synthase
LocationchrH02: 16864471 .. 16867776 (+)
RNA-Seq ExpressionChy2G039530
SyntenyChy2G039530
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTTGCCTCTACGGCGCTCAACTGCAAAATCCCCACCTTTTCACCCTCCTCTTCCCATTCAAATAACAAGATTTCTTCTTCTATCTCTCACTTTCCTGTCCGTTTCCGGCGAGTTTCCTCCAACCCATCGCCGGTTTTTCGGTCAAACGCCGTCTCCTGCACTTTGACTCGGGAGCCGTCGCTGGCAATGGAGGACAAAGTCAATAATTCGAGTCTGCAACAGAGACCGGATTCATTTGGCCGTTTTGGCCGTTTTGGGGGAAAATATGTCCCCGAGACCCTAATGCACGCCCTCACCGAGCTCGAAACTGCTTTCTTTTCCCTTGCTGGGGATCAAGACTTTCAGGTTTGTGGGAATTTTGTTTTCCTTTGAGATTTTTTCTTCTTTATTTTATTTTATTTAATTTTTAGATGGAGGAAGTTTAATTTCCTGAGTTTTGGGTCTTTGTTTTAATCGCCCAATTAGGTGCTATTTGAGGATTTTGAATCTCTTGAATGCTTATTGGATTTTATGTGGACTTAAGTTGAAATGTCTTCTTTTTTCTTTCTTGAAATTGGTAGTAGAAAAGTGGGAGTTTGACATTTATTAGACTCAGTTACATAATAAAACCATTAAGTATGTAGATGTTATTTTCAAAGAGAAGAAGGCTTTGAGACATTTTTTAATTTCATTGCAAAGTTCATGATTTTGAAATTCAGTAGTTTCTAAATTGGACAAACCAAGCAGTCCTTATTATCTTTTTCTACCCACTCTTTATAATAATTGACTCTAATCATCAGAAAGAGCTGGATGGAATTTTGAGAGACTACGTGGGGAGAGAGAGCCCTTTGTACTTCGCGGAGAGGCTGACTGAGCACTATAGACGTTCCAATGGTGAGGGGCCTCATATTTTCCTGAAGAGAGAAGACCTTAACCACACAGGGGCTCACAAAATTAACAATGCCGTTGCCCAAGCTTTGCTTGCTAAGCGTTTGGGAAAGAAACGGATTATAGCTGAAACAGGCGCAGGTCAGCATGGAGTTGCAACTGCAACTGTTTGTGCTAGATTTGGACTAGAATGTATCATCTACATGGGTGCCCAAGATATGGAGAGGCAAGCTCTCAATGTGTTCAGAATGCGGCTTCTTGGGGCTGAGGTTTGTCCATTATAATTTTGGTTGTTTTCTTCCATTATTAATCTGCAGCCATTTATTTGTAGGACTCTAATTTCATCGTCCAATCTGTTATGATCAATTTGGACCTTCAAAACTGTGAATATTGTATTTCTGTTAGAGAATCTGGGTGCTCAACACACCAGGAGAATTTATCTTATTTGTTTTCTTGGCTGAATGTTCATTTTTTTGCATATTTTAGATCCCTCTGATAACGTTTGTATTTATTACCATTCTACTGCATAGTGAGACATTCTCTTTAGTTATTCTCCTTTTTCATTGCTAGTTAGCATTCCCTAAAGTTATGGATTGAGCTTTCTCTGACTTGCATCCTTGCAATGAATATGATTTAAAGTGCAGCTTATTCAGTCAACAAATATTTATTGCATGTAATATAATTTGTTTCCTATTCTTTCCATTATTGTTTGTAGAGATAATTTTGAACCTGGTCACATTTCTTCCAAGTCTTTTCACGAACCGGCATTTGAAAATAAAAGATTTATCCTAATAGGTGTGATTCGCAGTTGCTACCTCATCCTAGGTGTTCAAAATGAATTTCAAAGTATAAATTACACTTGCTTTGCAGGTTAGGCCAGTTCATTCTGGTACTGCCACGCTAAAGGATGCTACTTCAGAAGCTATAAGGGACTGGGTGACTAATGTGGAGACTACCCATTATATCTTAGGATCTGTTGCTGGGCCCCACCCATATCCCATGATGGTCAGAGATTTTCATGCTGTGATTGGTAAAGAAACGAGAAAACAGGCCTTGGAGAAGTGGGGAGGGAAGCCAGATGTCCTAGTAGCATGTGTTGGAGGAGGTTCAAATGCTATGGGACTATTTCATGAGTTTGTCAATGACAAAGATGTTAGGTTGGTTGGTGTGGAGGCTGCAGGTTTTGGCTTGGATAGTGGTAAGCATGCAGCCACTCTAACCAAAGGGGAAGTTGGGGTTCTACATGGAGCGATGAGTTATTTACTACAGGATGATGATGGGCAAATAATTGAACCTCACTCGATCAGTGCAGGGTATGGGATATATACAAAGTTTTCCTTTGTCATGATGTTTGCCTGCTGTTCCAATCTTGCACCTTTCAAATTTAATCCATGTGTTTTTTTTCCGTGGTCTGCAGTTTGGATTACCCTGGGGTTGGACCAGAGCATAGTTATTTGAAAGATCTAGGCCGTGCTGAGTACCACAGTGTAACGGATGATGAAGCACTGGAAGGTACTGGATTGTAATACATTAATTTCTTGGTATTTTATTTGTTAGATATACCATCTATCTTTTGAAAAGTTATCTTTTAGATATTTATATATATATATATATATGTATATATCCTTCTTTTTCAGAGCCAAAGTACTCTTTTTATTCTGAAAATGACAATCTGTTTTTACTATTTAGGTAGTGTTTTATACTTAATACTTCAGTCCATTTTTTCTGCTCTGTTCAATGTTTTTCTTTCTTTCCTATCTTGATTCTCACCTTCCTTTTAGGTGGAAACTGCTATGCATTCTTTTCAGCTATTAGTAATTTTGTAAACTGTGCACTGGCACATGCTAAATCATGACCAATTATCGTGGTTCTTCTTTAGGTTCCTGATGAGAAATTATCTGTTCTCTCATTAAGTTTTAAATAAATTTGTACTAAATTAGTTTTACATAAAGTCCTATCTGTTACTACAATCTGAGCTCTGTTTCACCCACTGATTCCATGATCAATAATCTACTGCAGAGTCGATCTTGATAGTGGAATTGTTGGTCCTGAGGGAGGTTTCTCTTCCATGTATCTGTCTTTTGACTGATTTTGATTGTTATCTTGAATCACTATGGACTGTTTTGGAAACTTGTACATCCAATTAGAACTCGATTTCTGTCCAAGATGATGGTTGTGAAAGCTAGCTTTCACCATTTCTTGTTCTTGTTGTTCTTCTGATCCTTAGAGACTAAATTCATAATCTGGCTTGTGACTGCAGCCTTCAAGAGATTGTCACGACTGGAGGGCATAATCCCAGCTCTGGAGACATCTCATGCTCTGGCTTATCTTGAGAAGTTGTGTCTGACCCTCCCAGACGGAACAAAGGTCGTGCTTAACTGCAGCGGCAGAGGAGATAAGGATGTTCAGACAGCGATCAAGTATTTGCAAGTTTGA

mRNA sequence

ATGGTTGCCTCTACGGCGCTCAACTGCAAAATCCCCACCTTTTCACCCTCCTCTTCCCATTCAAATAACAAGATTTCTTCTTCTATCTCTCACTTTCCTGTCCGTTTCCGGCGAGTTTCCTCCAACCCATCGCCGGTTTTTCGGTCAAACGCCGTCTCCTGCACTTTGACTCGGGAGCCGTCGCTGGCAATGGAGGACAAAGTCAATAATTCGAGTCTGCAACAGAGACCGGATTCATTTGGCCGTTTTGGCCGTTTTGGGGGAAAATATGTCCCCGAGACCCTAATGCACGCCCTCACCGAGCTCGAAACTGCTTTCTTTTCCCTTGCTGGGGATCAAGACTTTCAGAAAGAGCTGGATGGAATTTTGAGAGACTACGTGGGGAGAGAGAGCCCTTTGTACTTCGCGGAGAGGCTGACTGAGCACTATAGACGTTCCAATGGTGAGGGGCCTCATATTTTCCTGAAGAGAGAAGACCTTAACCACACAGGGGCTCACAAAATTAACAATGCCGTTGCCCAAGCTTTGCTTGCTAAGCGTTTGGGAAAGAAACGGATTATAGCTGAAACAGGCGCAGGTCAGCATGGAGTTGCAACTGCAACTGTTTGTGCTAGATTTGGACTAGAATGTATCATCTACATGGGTGCCCAAGATATGGAGAGGCAAGCTCTCAATGTGTTCAGAATGCGGCTTCTTGGGGCTGAGGTTAGGCCAGTTCATTCTGGTACTGCCACGCTAAAGGATGCTACTTCAGAAGCTATAAGGGACTGGGTGACTAATGTGGAGACTACCCATTATATCTTAGGATCTGTTGCTGGGCCCCACCCATATCCCATGATGGTCAGAGATTTTCATGCTGTGATTGGTAAAGAAACGAGAAAACAGGCCTTGGAGAAGTGGGGAGGGAAGCCAGATGTCCTAGTAGCATGTGTTGGAGGAGGTTCAAATGCTATGGGACTATTTCATGAGTTTGTCAATGACAAAGATGTTAGGTTGGTTGGTGTGGAGGCTGCAGGTTTTGGCTTGGATAGTGGTAAGCATGCAGCCACTCTAACCAAAGGGGAAGTTGGGGTTCTACATGGAGCGATGAGTTATTTACTACAGGATGATGATGGGCAAATAATTGAACCTCACTCGATCAGTGCAGGTTTGGATTACCCTGGGGTTGGACCAGAGCATAGTTATTTGAAAGATCTAGGCCGTGCTGAGTACCACAGTGTAACGGATGATGAAGCACTGGAAGCCTTCAAGAGATTGTCACGACTGGAGGGCATAATCCCAGCTCTGGAGACATCTCATGCTCTGGCTTATCTTGAGAAGTTGTGTCTGACCCTCCCAGACGGAACAAAGGTCGTGCTTAACTGCAGCGGCAGAGGAGATAAGGATGTTCAGACAGCGATCAAGTATTTGCAAGTTTGA

Coding sequence (CDS)

ATGGTTGCCTCTACGGCGCTCAACTGCAAAATCCCCACCTTTTCACCCTCCTCTTCCCATTCAAATAACAAGATTTCTTCTTCTATCTCTCACTTTCCTGTCCGTTTCCGGCGAGTTTCCTCCAACCCATCGCCGGTTTTTCGGTCAAACGCCGTCTCCTGCACTTTGACTCGGGAGCCGTCGCTGGCAATGGAGGACAAAGTCAATAATTCGAGTCTGCAACAGAGACCGGATTCATTTGGCCGTTTTGGCCGTTTTGGGGGAAAATATGTCCCCGAGACCCTAATGCACGCCCTCACCGAGCTCGAAACTGCTTTCTTTTCCCTTGCTGGGGATCAAGACTTTCAGAAAGAGCTGGATGGAATTTTGAGAGACTACGTGGGGAGAGAGAGCCCTTTGTACTTCGCGGAGAGGCTGACTGAGCACTATAGACGTTCCAATGGTGAGGGGCCTCATATTTTCCTGAAGAGAGAAGACCTTAACCACACAGGGGCTCACAAAATTAACAATGCCGTTGCCCAAGCTTTGCTTGCTAAGCGTTTGGGAAAGAAACGGATTATAGCTGAAACAGGCGCAGGTCAGCATGGAGTTGCAACTGCAACTGTTTGTGCTAGATTTGGACTAGAATGTATCATCTACATGGGTGCCCAAGATATGGAGAGGCAAGCTCTCAATGTGTTCAGAATGCGGCTTCTTGGGGCTGAGGTTAGGCCAGTTCATTCTGGTACTGCCACGCTAAAGGATGCTACTTCAGAAGCTATAAGGGACTGGGTGACTAATGTGGAGACTACCCATTATATCTTAGGATCTGTTGCTGGGCCCCACCCATATCCCATGATGGTCAGAGATTTTCATGCTGTGATTGGTAAAGAAACGAGAAAACAGGCCTTGGAGAAGTGGGGAGGGAAGCCAGATGTCCTAGTAGCATGTGTTGGAGGAGGTTCAAATGCTATGGGACTATTTCATGAGTTTGTCAATGACAAAGATGTTAGGTTGGTTGGTGTGGAGGCTGCAGGTTTTGGCTTGGATAGTGGTAAGCATGCAGCCACTCTAACCAAAGGGGAAGTTGGGGTTCTACATGGAGCGATGAGTTATTTACTACAGGATGATGATGGGCAAATAATTGAACCTCACTCGATCAGTGCAGGTTTGGATTACCCTGGGGTTGGACCAGAGCATAGTTATTTGAAAGATCTAGGCCGTGCTGAGTACCACAGTGTAACGGATGATGAAGCACTGGAAGCCTTCAAGAGATTGTCACGACTGGAGGGCATAATCCCAGCTCTGGAGACATCTCATGCTCTGGCTTATCTTGAGAAGTTGTGTCTGACCCTCCCAGACGGAACAAAGGTCGTGCTTAACTGCAGCGGCAGAGGAGATAAGGATGTTCAGACAGCGATCAAGTATTTGCAAGTTTGA

Protein sequence

MVASTALNCKIPTFSPSSSHSNNKISSSISHFPVRFRRVSSNPSPVFRSNAVSCTLTREPSLAMEDKVNNSSLQQRPDSFGRFGRFGGKYVPETLMHALTELETAFFSLAGDQDFQKELDGILRDYVGRESPLYFAERLTEHYRRSNGEGPHIFLKREDLNHTGAHKINNAVAQALLAKRLGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMRLLGAEVRPVHSGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKWGGKPDVLVACVGGGSNAMGLFHEFVNDKDVRLVGVEAAGFGLDSGKHAATLTKGEVGVLHGAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSYLKDLGRAEYHSVTDDEALEAFKRLSRLEGIIPALETSHALAYLEKLCLTLPDGTKVVLNCSGRGDKDVQTAIKYLQV*
Homology
BLAST of Chy2G039530 vs. ExPASy Swiss-Prot
Match: P14671 (Tryptophan synthase beta chain 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TSB1 PE=2 SV=1)

HSP 1 Score: 769.2 bits (1985), Expect = 2.6e-221
Identity = 385/464 (82.97%), Postives = 417/464 (89.87%), Query Frame = 0

Query: 13  TFSPSSSHSNNKISSSISHFPVRFRRVSSNPSPVFRSN----AVSCTLTREPSLAMEDKV 72
           TF  S S + +  SS ++H    F+ V   P P  RS     +VSCT+ ++P + M    
Sbjct: 9   TFRASVSSAPSS-SSQLTHLKSPFKAVKYTPLPSSRSKSSSFSVSCTIAKDPPVLMA-AG 68

Query: 73  NNSSLQQRPDSFGRFGRFGGKYVPETLMHALTELETAFFSLAGDQDFQKELDGILRDYVG 132
           ++ +L QRPDSFGRFG+FGGKYVPETLMHAL+ELE+AF++LA D DFQ+EL GIL+DYVG
Sbjct: 69  SDPALWQRPDSFGRFGKFGGKYVPETLMHALSELESAFYALATDDDFQRELAGILKDYVG 128

Query: 133 RESPLYFAERLTEHYRRSNGEGPHIFLKREDLNHTGAHKINNAVAQALLAKRLGKKRIIA 192
           RESPLYFAERLTEHYRR NGEGP I+LKREDLNHTGAHKINNAVAQALLAKRLGKKRIIA
Sbjct: 129 RESPLYFAERLTEHYRRENGEGPLIYLKREDLNHTGAHKINNAVAQALLAKRLGKKRIIA 188

Query: 193 ETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMRLLGAEVRPVHSGTATLKD 252
           ETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMRLLGAEVR VHSGTATLKD
Sbjct: 189 ETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMRLLGAEVRGVHSGTATLKD 248

Query: 253 ATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKWGGKPDVLV 312
           ATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKWGGKPDVLV
Sbjct: 249 ATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKWGGKPDVLV 308

Query: 313 ACVGGGSNAMGLFHEFVNDKDVRLVGVEAAGFGLDSGKHAATLTKGEVGVLHGAMSYLLQ 372
           ACVGGGSNAMGLFHEFVND +VR++GVEAAGFGLDSGKHAATLTKG+VGVLHGAMSYLLQ
Sbjct: 309 ACVGGGSNAMGLFHEFVNDTEVRMIGVEAAGFGLDSGKHAATLTKGDVGVLHGAMSYLLQ 368

Query: 373 DDDGQIIEPHSISAGLDYPGVGPEHSYLKDLGRAEYHSVTDDEALEAFKRLSRLEGIIPA 432
           DDDGQIIEPHSISAGLDYPGVGPEHS+ KD+GRAEY+S+TD+EALEAFKR+SRLEGIIPA
Sbjct: 369 DDDGQIIEPHSISAGLDYPGVGPEHSFFKDMGRAEYYSITDEEALEAFKRVSRLEGIIPA 428

Query: 433 LETSHALAYLEKLCLTLPDGTKVVLNCSGRGDKDVQTAIKYLQV 473
           LETSHALAYLEKLC TL DGT+VVLN SGRGDKDVQT  KYL V
Sbjct: 429 LETSHALAYLEKLCPTLSDGTRVVLNFSGRGDKDVQTVAKYLDV 470

BLAST of Chy2G039530 vs. ExPASy Swiss-Prot
Match: P25269 (Tryptophan synthase beta chain 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TSB2 PE=1 SV=2)

HSP 1 Score: 768.1 bits (1982), Expect = 5.9e-221
Identity = 384/469 (81.88%), Postives = 425/469 (90.62%), Query Frame = 0

Query: 13  TFSPSSSHSNNKISSSISHFPVRFRRVSSNPSPVFRSN---AVSCTLTREPSLAMEDKV- 72
           TF PSS  ++++++   S  P +  + +  PS   RS+   +VSCT+ ++P++ M D   
Sbjct: 9   TFRPSSVSASSELTHLRS--PSKLPKFTPLPSARSRSSSSFSVSCTIAKDPAVVMADSEK 68

Query: 73  -----NNSSLQQRPDSFGRFGRFGGKYVPETLMHALTELETAFFSLAGDQDFQKELDGIL 132
                ++ ++ QRPDSFGRFG+FGGKYVPETLMHAL+ELETAF+SLA D+DFQ+EL  IL
Sbjct: 69  IKAAGSDPTMWQRPDSFGRFGKFGGKYVPETLMHALSELETAFYSLATDEDFQRELAEIL 128

Query: 133 RDYVGRESPLYFAERLTEHYRRSNGEGPHIFLKREDLNHTGAHKINNAVAQALLAKRLGK 192
           +DYVGRESPLYFAERLTEHYRR NGEGP I+LKREDLNHTGAHKINNAVAQALLAKRLGK
Sbjct: 129 KDYVGRESPLYFAERLTEHYRRENGEGPLIYLKREDLNHTGAHKINNAVAQALLAKRLGK 188

Query: 193 KRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMRLLGAEVRPVHSGT 252
           KRIIAETGAGQHGVATATVCARFGL+CIIYMGAQDMERQALNVFRMRLLGAEVR VHSGT
Sbjct: 189 KRIIAETGAGQHGVATATVCARFGLQCIIYMGAQDMERQALNVFRMRLLGAEVRGVHSGT 248

Query: 253 ATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKWGGK 312
           ATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQA+EKWGGK
Sbjct: 249 ATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQAMEKWGGK 308

Query: 313 PDVLVACVGGGSNAMGLFHEFVNDKDVRLVGVEAAGFGLDSGKHAATLTKGEVGVLHGAM 372
           PDVLVACVGGGSNAMGLFHEFV+D +VR++GVEAAGFGLDSGKHAATLTKG+VGVLHGAM
Sbjct: 309 PDVLVACVGGGSNAMGLFHEFVDDTEVRMIGVEAAGFGLDSGKHAATLTKGDVGVLHGAM 368

Query: 373 SYLLQDDDGQIIEPHSISAGLDYPGVGPEHSYLKDLGRAEYHSVTDDEALEAFKRLSRLE 432
           SYLLQDDDGQIIEPHSISAGLDYPGVGPEHS+LKD+GRAEY SVTD+EALEAFKR+SRLE
Sbjct: 369 SYLLQDDDGQIIEPHSISAGLDYPGVGPEHSFLKDVGRAEYFSVTDEEALEAFKRVSRLE 428

Query: 433 GIIPALETSHALAYLEKLCLTLPDGTKVVLNCSGRGDKDVQTAIKYLQV 473
           GIIPALETSHALA+LEKLC TLPDG +VVLN SGRGDKDVQTAIKYL+V
Sbjct: 429 GIIPALETSHALAHLEKLCPTLPDGARVVLNFSGRGDKDVQTAIKYLEV 475

BLAST of Chy2G039530 vs. ExPASy Swiss-Prot
Match: O50046 (Tryptophan synthase beta chain 2, chloroplastic OS=Camptotheca acuminata OX=16922 GN=TSB PE=2 SV=1)

HSP 1 Score: 760.8 bits (1963), Expect = 9.4e-219
Identity = 381/466 (81.76%), Postives = 413/466 (88.63%), Query Frame = 0

Query: 20  HSNNKISSSISHFPVRFRRVSSNPS-------------PVFRSNAVSCTLTREPSLAMED 79
           ++N    ++ S FP  +R   SNPS                +  ++ CT+ RE  +  E 
Sbjct: 4   YTNPACRTNTSAFPGPYRPY-SNPSRFSFNLDKFRPRTSAIKVPSICCTIARE--MEKER 63

Query: 80  KVNNSSLQQRPDSFGRFGRFGGKYVPETLMHALTELETAFFSLAGDQDFQKELDGILRDY 139
                 + QRPDSFGRFG+FGGKYVPETLM+ALTELE+AF SL+GDQ FQKELDGIL+DY
Sbjct: 64  SEREPDVLQRPDSFGRFGKFGGKYVPETLMYALTELESAFRSLSGDQVFQKELDGILKDY 123

Query: 140 VGRESPLYFAERLTEHYRRSNGEGPHIFLKREDLNHTGAHKINNAVAQALLAKRLGKKRI 199
           VGRESPLYFAERLT HY+R NGEGP I+LKREDLNHTGAHKINNAVAQALLAKRLGKKRI
Sbjct: 124 VGRESPLYFAERLTLHYKRPNGEGPEIYLKREDLNHTGAHKINNAVAQALLAKRLGKKRI 183

Query: 200 IAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMRLLGAEVRPVHSGTATL 259
           IAETGAGQHGVATATVCARFGL+C+IYMGAQDMERQALNVFRMRLLGAEVR VHSGTATL
Sbjct: 184 IAETGAGQHGVATATVCARFGLQCVIYMGAQDMERQALNVFRMRLLGAEVRAVHSGTATL 243

Query: 260 KDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKWGGKPDV 319
           KDATSEAIRDWVTNVE+THYILGSVAGPHPYPMMVR+FHAVIGKETRKQALEKWGGKPDV
Sbjct: 244 KDATSEAIRDWVTNVESTHYILGSVAGPHPYPMMVREFHAVIGKETRKQALEKWGGKPDV 303

Query: 320 LVACVGGGSNAMGLFHEFVNDKDVRLVGVEAAGFGLDSGKHAATLTKGEVGVLHGAMSYL 379
           LVACVGGGSNAMGLFHEFV+DKDVR++GVEAAGFGLDSGKHAATLTKGEVGVLHGAMSYL
Sbjct: 304 LVACVGGGSNAMGLFHEFVDDKDVRMIGVEAAGFGLDSGKHAATLTKGEVGVLHGAMSYL 363

Query: 380 LQDDDGQIIEPHSISAGLDYPGVGPEHSYLKDLGRAEYHSVTDDEALEAFKRLSRLEGII 439
           LQDDDGQIIEPHSISAGLDYPGVGPEHS+LKD+GRAEY+  TD+EALEAFKRLSRLEGII
Sbjct: 364 LQDDDGQIIEPHSISAGLDYPGVGPEHSFLKDIGRAEYYCCTDEEALEAFKRLSRLEGII 423

Query: 440 PALETSHALAYLEKLCLTLPDGTKVVLNCSGRGDKDVQTAIKYLQV 473
           PALETSHALA+LEKLC TLP+GTKVVLNCSGRGDKDV TAIK+LQV
Sbjct: 424 PALETSHALAFLEKLCPTLPNGTKVVLNCSGRGDKDVHTAIKHLQV 466

BLAST of Chy2G039530 vs. ExPASy Swiss-Prot
Match: P43284 (Tryptophan synthase beta chain 2, chloroplastic (Fragment) OS=Zea mays OX=4577 GN=TSB2 PE=2 SV=1)

HSP 1 Score: 728.4 bits (1879), Expect = 5.2e-209
Identity = 356/425 (83.76%), Postives = 388/425 (91.29%), Query Frame = 0

Query: 50  NAVSCTLTREPSLAMEDKVNNSSLQ--QRPDSFGRFGRFGGKYVPETLMHALTELETAFF 109
           NA    +  E S A  +  N ++    QRPD+ GRFGRFGGKYVPETLMHALTELE+AF 
Sbjct: 19  NAAGQAVAAEASPAAVEMGNGAAAPGLQRPDAMGRFGRFGGKYVPETLMHALTELESAFH 78

Query: 110 SLAGDQDFQKELDGILRDYVGRESPLYFAERLTEHYRRSNGEGPHIFLKREDLNHTGAHK 169
           +LA D +FQKELDGIL+DYVGRESPLYFAERLTEHY+R++G GP I+LKREDLNHTGAHK
Sbjct: 79  ALATDDEFQKELDGILKDYVGRESPLYFAERLTEHYKRADGTGPLIYLKREDLNHTGAHK 138

Query: 170 INNAVAQALLAKRLGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVF 229
           INNAVAQALLAKRLGK+RIIAETGAGQHGVATATVC RFGL+CIIYMGAQDMERQALNVF
Sbjct: 139 INNAVAQALLAKRLGKQRIIAETGAGQHGVATATVCRRFGLQCIIYMGAQDMERQALNVF 198

Query: 230 RMRLLGAEVRPVHSGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAV 289
           RMRLLGAEVR VHSGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVR+FH V
Sbjct: 199 RMRLLGAEVRAVHSGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVREFHKV 258

Query: 290 IGKETRKQALEKWGGKPDVLVACVGGGSNAMGLFHEFVNDKDVRLVGVEAAGFGLDSGKH 349
           IGKETR+QA++KWGGKPDVLVACVGGGSNAMGLFHEFV D+DVRLVG+EAAG G+D+ KH
Sbjct: 259 IGKETRRQAMDKWGGKPDVLVACVGGGSNAMGLFHEFVEDQDVRLVGLEAAGHGVDTDKH 318

Query: 350 AATLTKGEVGVLHGAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSYLKDLGRAEYHSV 409
           AATLTKG+VGVLHG+MSYLLQDDDGQ+IEPHSISAGLDYPGVGPEHS+LKD+GRAEY SV
Sbjct: 319 AATLTKGQVGVLHGSMSYLLQDDDGQVIEPHSISAGLDYPGVGPEHSFLKDIGRAEYDSV 378

Query: 410 TDDEALEAFKRLSRLEGIIPALETSHALAYLEKLCLTLPDGTKVVLNCSGRGDKDVQTAI 469
           TD EAL+AFKR+SRLEGIIPALETSHALAYLEKLC TL DG +VV+NCSGRGDKDV TA 
Sbjct: 379 TDQEALDAFKRVSRLEGIIPALETSHALAYLEKLCPTLADGVRVVVNCSGRGDKDVHTAS 438

Query: 470 KYLQV 473
           KYL V
Sbjct: 439 KYLDV 443

BLAST of Chy2G039530 vs. ExPASy Swiss-Prot
Match: P43283 (Tryptophan synthase beta chain 1 (Fragment) OS=Zea mays OX=4577 GN=TSB1 PE=2 SV=1)

HSP 1 Score: 713.8 bits (1841), Expect = 1.3e-204
Identity = 344/389 (88.43%), Postives = 369/389 (94.86%), Query Frame = 0

Query: 84  GRFGGKYVPETLMHALTELETAFFSLAGDQDFQKELDGILRDYVGRESPLYFAERLTEHY 143
           GRFGGKYVPETLMHALTELE AF +LA D +FQKELDGIL+DYVGRESPLYFAERLTEHY
Sbjct: 1   GRFGGKYVPETLMHALTELENAFHALATDDEFQKELDGILKDYVGRESPLYFAERLTEHY 60

Query: 144 RRSNGEGPHIFLKREDLNHTGAHKINNAVAQALLAKRLGKKRIIAETGAGQHGVATATVC 203
           +R++G GP I+LKREDLNH GAHKINNAVAQALLAKRLGK+RIIAETGAGQHGVATATVC
Sbjct: 61  KRADGTGPLIYLKREDLNHRGAHKINNAVAQALLAKRLGKQRIIAETGAGQHGVATATVC 120

Query: 204 ARFGLECIIYMGAQDMERQALNVFRMRLLGAEVRPVHSGTATLKDATSEAIRDWVTNVET 263
           ARFGL+CIIYMGAQDMERQALNVFRM+LLGAEVR VHSGTATLKDATSEAIRDWVTNVET
Sbjct: 121 ARFGLQCIIYMGAQDMERQALNVFRMKLLGAEVRAVHSGTATLKDATSEAIRDWVTNVET 180

Query: 264 THYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKWGGKPDVLVACVGGGSNAMGLFHE 323
           THYILGSVAGPHPYPMMVR+FH VIGKETR+QA+ KWGGKPDVLVACVGGGSNAMGLFHE
Sbjct: 181 THYILGSVAGPHPYPMMVREFHKVIGKETRRQAMHKWGGKPDVLVACVGGGSNAMGLFHE 240

Query: 324 FVNDKDVRLVGVEAAGFGLDSGKHAATLTKGEVGVLHGAMSYLLQDDDGQIIEPHSISAG 383
           FV D+DVRL+GVEAAG G+D+ KHAATLTKG+VGVLHG+MSYLLQDDDGQ+IEPHSISAG
Sbjct: 241 FVEDQDVRLIGVEAAGHGVDTDKHAATLTKGQVGVLHGSMSYLLQDDDGQVIEPHSISAG 300

Query: 384 LDYPGVGPEHSYLKDLGRAEYHSVTDDEALEAFKRLSRLEGIIPALETSHALAYLEKLCL 443
           LDYPGVGPEHS+LKD+GRAEY SVTD EAL+AFKR+SRLEGIIPALETSHALAYLEKLC 
Sbjct: 301 LDYPGVGPEHSFLKDIGRAEYDSVTDQEALDAFKRVSRLEGIIPALETSHALAYLEKLCP 360

Query: 444 TLPDGTKVVLNCSGRGDKDVQTAIKYLQV 473
           TLPDG +VVLNCSGRGDKDV TA KYL V
Sbjct: 361 TLPDGVRVVLNCSGRGDKDVHTASKYLDV 389

BLAST of Chy2G039530 vs. ExPASy TrEMBL
Match: A0A0A0M065 (Tryptophan synthase OS=Cucumis sativus OX=3659 GN=Csa_1G660140 PE=3 SV=1)

HSP 1 Score: 938.7 bits (2425), Expect = 9.3e-270
Identity = 465/472 (98.52%), Postives = 470/472 (99.58%), Query Frame = 0

Query: 1   MVASTALNCKIPTFSPSSSHSNNKISSSISHFPVRFRRVSSNPSPVFRSNAVSCTLTREP 60
           MVASTALNCKIPTFSPSSSHSNNKISSS SHFPVRFRRVSSNPSPVFRSNAVSCTLTREP
Sbjct: 1   MVASTALNCKIPTFSPSSSHSNNKISSSTSHFPVRFRRVSSNPSPVFRSNAVSCTLTREP 60

Query: 61  SLAMEDKVNNSSLQQRPDSFGRFGRFGGKYVPETLMHALTELETAFFSLAGDQDFQKELD 120
           SLAMEDKV++SSLQQRPDSFGRFGRFGGKYVPETLMHALTELETAF+SLAGDQDFQKELD
Sbjct: 61  SLAMEDKVHDSSLQQRPDSFGRFGRFGGKYVPETLMHALTELETAFYSLAGDQDFQKELD 120

Query: 121 GILRDYVGRESPLYFAERLTEHYRRSNGEGPHIFLKREDLNHTGAHKINNAVAQALLAKR 180
           GILRDYVGRESPLYFAERLTEHYRRSNGEGPHIFLKREDLNHTGAHKINNAVAQALLAKR
Sbjct: 121 GILRDYVGRESPLYFAERLTEHYRRSNGEGPHIFLKREDLNHTGAHKINNAVAQALLAKR 180

Query: 181 LGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMRLLGAEVRPVH 240
           LGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRM+LLGAEVRPVH
Sbjct: 181 LGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMQLLGAEVRPVH 240

Query: 241 SGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKW 300
           SGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKW
Sbjct: 241 SGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKW 300

Query: 301 GGKPDVLVACVGGGSNAMGLFHEFVNDKDVRLVGVEAAGFGLDSGKHAATLTKGEVGVLH 360
           GGKPDVLVACVGGGSNAMGLFHEFVNDKDVRLVGVEAAGFGLDSGKHAATLTKGEVGVLH
Sbjct: 301 GGKPDVLVACVGGGSNAMGLFHEFVNDKDVRLVGVEAAGFGLDSGKHAATLTKGEVGVLH 360

Query: 361 GAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSYLKDLGRAEYHSVTDDEALEAFKRLS 420
           GAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSYLKDLGRAEYHSVTDDEALEAFKRL+
Sbjct: 361 GAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSYLKDLGRAEYHSVTDDEALEAFKRLA 420

Query: 421 RLEGIIPALETSHALAYLEKLCLTLPDGTKVVLNCSGRGDKDVQTAIKYLQV 473
           RLEGIIPALETSHALAYLEKLC TLPDGTKVVLNCSGRGDKDVQTAIKYLQV
Sbjct: 421 RLEGIIPALETSHALAYLEKLCPTLPDGTKVVLNCSGRGDKDVQTAIKYLQV 472

BLAST of Chy2G039530 vs. ExPASy TrEMBL
Match: A0A1S3C016 (Tryptophan synthase OS=Cucumis melo OX=3656 GN=LOC103494920 PE=3 SV=1)

HSP 1 Score: 926.4 bits (2393), Expect = 4.8e-266
Identity = 459/472 (97.25%), Postives = 464/472 (98.31%), Query Frame = 0

Query: 1   MVASTALNCKIPTFSPSSSHSNNKISSSISHFPVRFRRVSSNPSPVFRSNAVSCTLTREP 60
           MVASTALNCKIPT SPS SHSNNKISSSI HFPVRFRRVSSNPSP+ RSNAVSCTLTREP
Sbjct: 1   MVASTALNCKIPTISPSPSHSNNKISSSIPHFPVRFRRVSSNPSPLLRSNAVSCTLTREP 60

Query: 61  SLAMEDKVNNSSLQQRPDSFGRFGRFGGKYVPETLMHALTELETAFFSLAGDQDFQKELD 120
           SLAMEDK++  SLQQRPDSFGRFGRFGGKYVPETLMHALTELE AF+SLAGDQDFQKELD
Sbjct: 61  SLAMEDKLHTLSLQQRPDSFGRFGRFGGKYVPETLMHALTELEAAFYSLAGDQDFQKELD 120

Query: 121 GILRDYVGRESPLYFAERLTEHYRRSNGEGPHIFLKREDLNHTGAHKINNAVAQALLAKR 180
           GILRDYVGRESPLYFAERLTEHYRRSNGEGPHIFLKREDLNHTGAHKINNAVAQALLAKR
Sbjct: 121 GILRDYVGRESPLYFAERLTEHYRRSNGEGPHIFLKREDLNHTGAHKINNAVAQALLAKR 180

Query: 181 LGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMRLLGAEVRPVH 240
           LGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMRLLGAEVRPVH
Sbjct: 181 LGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMRLLGAEVRPVH 240

Query: 241 SGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKW 300
           SGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKW
Sbjct: 241 SGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKW 300

Query: 301 GGKPDVLVACVGGGSNAMGLFHEFVNDKDVRLVGVEAAGFGLDSGKHAATLTKGEVGVLH 360
           GGKPDVLVACVGGGSNAMGLFHEFVND+DVRLVGVEAAGFGLDSGKHAATLTKGEVGVLH
Sbjct: 301 GGKPDVLVACVGGGSNAMGLFHEFVNDEDVRLVGVEAAGFGLDSGKHAATLTKGEVGVLH 360

Query: 361 GAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSYLKDLGRAEYHSVTDDEALEAFKRLS 420
           GAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSYLKDLGRAEYHSVTDDEALEAFKRLS
Sbjct: 361 GAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSYLKDLGRAEYHSVTDDEALEAFKRLS 420

Query: 421 RLEGIIPALETSHALAYLEKLCLTLPDGTKVVLNCSGRGDKDVQTAIKYLQV 473
           RLEGIIPALETSHALAYLEKLC TLPDGTKVVLNCSGRGDKDVQTAIKYLQV
Sbjct: 421 RLEGIIPALETSHALAYLEKLCPTLPDGTKVVLNCSGRGDKDVQTAIKYLQV 472

BLAST of Chy2G039530 vs. ExPASy TrEMBL
Match: A0A5D3E225 (Tryptophan synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold588G00030 PE=3 SV=1)

HSP 1 Score: 917.9 bits (2371), Expect = 1.7e-263
Identity = 459/483 (95.03%), Postives = 464/483 (96.07%), Query Frame = 0

Query: 1   MVASTALNCKIPTFSPSSSHSNNKISSSISHFPVRFRRVSSNPSPVFRSNAVSCTLTREP 60
           MVASTALNCKIPT SPS SHSNNKISSSI HFPVRFRRVSSNPSP+ RSNAVSCTLTREP
Sbjct: 1   MVASTALNCKIPTISPSPSHSNNKISSSIPHFPVRFRRVSSNPSPLLRSNAVSCTLTREP 60

Query: 61  SLAMEDKVNNSSLQQRPDSFGRFGRFGGKYVPETLMHALTELETAFFSLAGDQDFQKELD 120
           SLAMEDK++  SLQQRPDSFGRFGRFGGKYVPETLMHALTELE AF+SLAGDQDFQKELD
Sbjct: 61  SLAMEDKLHTLSLQQRPDSFGRFGRFGGKYVPETLMHALTELEAAFYSLAGDQDFQKELD 120

Query: 121 GILRDYVGRESPLYFAERLTEHYRRSNGEGPHIFLKREDLNHTGAHKINNAVAQALLAKR 180
           GILRDYVGRESPLYFAERLTEHYRRSNGEGPHIFLKREDLNHTGAHKINNAVAQALLAKR
Sbjct: 121 GILRDYVGRESPLYFAERLTEHYRRSNGEGPHIFLKREDLNHTGAHKINNAVAQALLAKR 180

Query: 181 LGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMRLLGAEVRPVH 240
           LGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMRLLGAEVRPVH
Sbjct: 181 LGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMRLLGAEVRPVH 240

Query: 241 SGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKW 300
           SGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKW
Sbjct: 241 SGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKW 300

Query: 301 GGKPDVLVACVGGGSNAMGLFHEFVNDKDVRLVGVEAAGFGLDSGKHAATLTKGEVGVLH 360
           GGKPDVLVACVGGGSNAMGLFHEFVND+DVRLVGVEAAGFGLDSGKHAATLTKGEVGVLH
Sbjct: 301 GGKPDVLVACVGGGSNAMGLFHEFVNDEDVRLVGVEAAGFGLDSGKHAATLTKGEVGVLH 360

Query: 361 GAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSYLKDLGRAEYHSVTDDEALE------ 420
           GAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSYLKDLGRAEYHSVTDDEALE      
Sbjct: 361 GAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSYLKDLGRAEYHSVTDDEALEEGKFII 420

Query: 421 -----AFKRLSRLEGIIPALETSHALAYLEKLCLTLPDGTKVVLNCSGRGDKDVQTAIKY 473
                AFKRLSRLEGIIPALETSHALAYLEKLC TLPDGTKVVLNCSGRGDKDVQTAIKY
Sbjct: 421 WLVTAAFKRLSRLEGIIPALETSHALAYLEKLCPTLPDGTKVVLNCSGRGDKDVQTAIKY 480

BLAST of Chy2G039530 vs. ExPASy TrEMBL
Match: A0A6J1CDB2 (Tryptophan synthase OS=Momordica charantia OX=3673 GN=LOC111010430 PE=3 SV=1)

HSP 1 Score: 875.2 bits (2260), Expect = 1.3e-250
Identity = 436/472 (92.37%), Postives = 446/472 (94.49%), Query Frame = 0

Query: 1   MVASTALNCKIPTFSPSSSHSNNKISSSISHFPVRFRRVSSNPSPVFRSNAVSCTLTREP 60
           MVA TALNCKIPT SPSSSHSN++ISSS S  P+RFRR +S PSP  R   VSCTLT +P
Sbjct: 1   MVAFTALNCKIPTVSPSSSHSNSEISSSFSRLPLRFRRAASYPSPAARRCGVSCTLTTQP 60

Query: 61  SLAMEDKVNNSSLQQRPDSFGRFGRFGGKYVPETLMHALTELETAFFSLAGDQDFQKELD 120
           SLAMEDK       QRPDSFGRFGRFGGKYVPETLMHALT+LE AF SLAGDQ+FQKELD
Sbjct: 61  SLAMEDKTQT----QRPDSFGRFGRFGGKYVPETLMHALTQLEAAFHSLAGDQEFQKELD 120

Query: 121 GILRDYVGRESPLYFAERLTEHYRRSNGEGPHIFLKREDLNHTGAHKINNAVAQALLAKR 180
           GILRDYVGRESPLYFAERLTEHYRR NGEGPHIFLKREDLNHTGAHKINNAVAQALLAKR
Sbjct: 121 GILRDYVGRESPLYFAERLTEHYRRPNGEGPHIFLKREDLNHTGAHKINNAVAQALLAKR 180

Query: 181 LGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMRLLGAEVRPVH 240
           LGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMRLLGAEV+PVH
Sbjct: 181 LGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMRLLGAEVKPVH 240

Query: 241 SGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKW 300
           SGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKW
Sbjct: 241 SGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKW 300

Query: 301 GGKPDVLVACVGGGSNAMGLFHEFVNDKDVRLVGVEAAGFGLDSGKHAATLTKGEVGVLH 360
           GGKPDVLVACVGGGSNAMGLFHEFV DKDVRLVGVEAAGFGLDSGKHAATLTKGE+GVLH
Sbjct: 301 GGKPDVLVACVGGGSNAMGLFHEFVGDKDVRLVGVEAAGFGLDSGKHAATLTKGEIGVLH 360

Query: 361 GAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSYLKDLGRAEYHSVTDDEALEAFKRLS 420
           GAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSYLKDLGRAEY+SVTDDEALEAFKRLS
Sbjct: 361 GAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSYLKDLGRAEYYSVTDDEALEAFKRLS 420

Query: 421 RLEGIIPALETSHALAYLEKLCLTLPDGTKVVLNCSGRGDKDVQTAIKYLQV 473
           RLEGIIPALETSHALAYLEKLC TLPDGTKVVLNCSGRGDKDV TAIKYLQV
Sbjct: 421 RLEGIIPALETSHALAYLEKLCPTLPDGTKVVLNCSGRGDKDVHTAIKYLQV 468

BLAST of Chy2G039530 vs. ExPASy TrEMBL
Match: A0A6J1J261 (Tryptophan synthase OS=Cucurbita maxima OX=3661 GN=LOC111480575 PE=3 SV=1)

HSP 1 Score: 867.1 bits (2239), Expect = 3.4e-248
Identity = 434/472 (91.95%), Postives = 444/472 (94.07%), Query Frame = 0

Query: 1   MVASTALNCKIPTFSPSSSHSNNKISSSISHFPVRFRRVSSNPSPVFRSNAVSCTLTREP 60
           MVAS  L+CKI T  PSSSHSN+KISSS S FPV  RR   NPSPVFRS AV+CTLTRE 
Sbjct: 1   MVASVELSCKILTIPPSSSHSNSKISSSFSRFPVHSRRFLQNPSPVFRSCAVTCTLTRET 60

Query: 61  SLAMEDKVNNSSLQQRPDSFGRFGRFGGKYVPETLMHALTELETAFFSLAGDQDFQKELD 120
           SLAMED     +L QRPDSFGRFGRFGGKYVPETLMHAL ELETAF SL+GDQ+FQKELD
Sbjct: 61  SLAMEDN-KTQNLGQRPDSFGRFGRFGGKYVPETLMHALAELETAFHSLSGDQEFQKELD 120

Query: 121 GILRDYVGRESPLYFAERLTEHYRRSNGEGPHIFLKREDLNHTGAHKINNAVAQALLAKR 180
           GILRDYVGR SPLYFAERLTEHYRR NGEGPHIFLKREDLNHTGAHKINNAVAQALLAKR
Sbjct: 121 GILRDYVGRASPLYFAERLTEHYRRPNGEGPHIFLKREDLNHTGAHKINNAVAQALLAKR 180

Query: 181 LGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMRLLGAEVRPVH 240
           LGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMRLLGAEVRPVH
Sbjct: 181 LGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMRLLGAEVRPVH 240

Query: 241 SGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKW 300
           SGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKW
Sbjct: 241 SGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKW 300

Query: 301 GGKPDVLVACVGGGSNAMGLFHEFVNDKDVRLVGVEAAGFGLDSGKHAATLTKGEVGVLH 360
           GGKPDVLVACVGGGSNAMGLFHEFVND+DVRLVGVEAAGFG+DSGKHAATLTKGE+GVLH
Sbjct: 301 GGKPDVLVACVGGGSNAMGLFHEFVNDEDVRLVGVEAAGFGVDSGKHAATLTKGEIGVLH 360

Query: 361 GAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSYLKDLGRAEYHSVTDDEALEAFKRLS 420
           GAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSYLKD GRAEY+SVTDDEALEAFKRLS
Sbjct: 361 GAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSYLKDAGRAEYYSVTDDEALEAFKRLS 420

Query: 421 RLEGIIPALETSHALAYLEKLCLTLPDGTKVVLNCSGRGDKDVQTAIKYLQV 473
           RLEGIIPALETSHALAYLEKLC TL DGTKVVLNCSGRGDKDV TAIKYLQV
Sbjct: 421 RLEGIIPALETSHALAYLEKLCPTLADGTKVVLNCSGRGDKDVHTAIKYLQV 471

BLAST of Chy2G039530 vs. NCBI nr
Match: XP_004142558.1 (tryptophan synthase beta chain 1 [Cucumis sativus] >KGN66694.1 hypothetical protein Csa_007090 [Cucumis sativus])

HSP 1 Score: 932 bits (2408), Expect = 0.0
Identity = 465/472 (98.52%), Postives = 470/472 (99.58%), Query Frame = 0

Query: 1   MVASTALNCKIPTFSPSSSHSNNKISSSISHFPVRFRRVSSNPSPVFRSNAVSCTLTREP 60
           MVASTALNCKIPTFSPSSSHSNNKISSS SHFPVRFRRVSSNPSPVFRSNAVSCTLTREP
Sbjct: 1   MVASTALNCKIPTFSPSSSHSNNKISSSTSHFPVRFRRVSSNPSPVFRSNAVSCTLTREP 60

Query: 61  SLAMEDKVNNSSLQQRPDSFGRFGRFGGKYVPETLMHALTELETAFFSLAGDQDFQKELD 120
           SLAMEDKV++SSLQQRPDSFGRFGRFGGKYVPETLMHALTELETAF+SLAGDQDFQKELD
Sbjct: 61  SLAMEDKVHDSSLQQRPDSFGRFGRFGGKYVPETLMHALTELETAFYSLAGDQDFQKELD 120

Query: 121 GILRDYVGRESPLYFAERLTEHYRRSNGEGPHIFLKREDLNHTGAHKINNAVAQALLAKR 180
           GILRDYVGRESPLYFAERLTEHYRRSNGEGPHIFLKREDLNHTGAHKINNAVAQALLAKR
Sbjct: 121 GILRDYVGRESPLYFAERLTEHYRRSNGEGPHIFLKREDLNHTGAHKINNAVAQALLAKR 180

Query: 181 LGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMRLLGAEVRPVH 240
           LGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRM+LLGAEVRPVH
Sbjct: 181 LGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMQLLGAEVRPVH 240

Query: 241 SGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKW 300
           SGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKW
Sbjct: 241 SGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKW 300

Query: 301 GGKPDVLVACVGGGSNAMGLFHEFVNDKDVRLVGVEAAGFGLDSGKHAATLTKGEVGVLH 360
           GGKPDVLVACVGGGSNAMGLFHEFVNDKDVRLVGVEAAGFGLDSGKHAATLTKGEVGVLH
Sbjct: 301 GGKPDVLVACVGGGSNAMGLFHEFVNDKDVRLVGVEAAGFGLDSGKHAATLTKGEVGVLH 360

Query: 361 GAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSYLKDLGRAEYHSVTDDEALEAFKRLS 420
           GAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSYLKDLGRAEYHSVTDDEALEAFKRL+
Sbjct: 361 GAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSYLKDLGRAEYHSVTDDEALEAFKRLA 420

Query: 421 RLEGIIPALETSHALAYLEKLCLTLPDGTKVVLNCSGRGDKDVQTAIKYLQV 472
           RLEGIIPALETSHALAYLEKLC TLPDGTKVVLNCSGRGDKDVQTAIKYLQV
Sbjct: 421 RLEGIIPALETSHALAYLEKLCPTLPDGTKVVLNCSGRGDKDVQTAIKYLQV 472

BLAST of Chy2G039530 vs. NCBI nr
Match: XP_008454527.1 (PREDICTED: tryptophan synthase beta chain 1 [Cucumis melo])

HSP 1 Score: 919 bits (2376), Expect = 0.0
Identity = 459/472 (97.25%), Postives = 464/472 (98.31%), Query Frame = 0

Query: 1   MVASTALNCKIPTFSPSSSHSNNKISSSISHFPVRFRRVSSNPSPVFRSNAVSCTLTREP 60
           MVASTALNCKIPT SPS SHSNNKISSSI HFPVRFRRVSSNPSP+ RSNAVSCTLTREP
Sbjct: 1   MVASTALNCKIPTISPSPSHSNNKISSSIPHFPVRFRRVSSNPSPLLRSNAVSCTLTREP 60

Query: 61  SLAMEDKVNNSSLQQRPDSFGRFGRFGGKYVPETLMHALTELETAFFSLAGDQDFQKELD 120
           SLAMEDK++  SLQQRPDSFGRFGRFGGKYVPETLMHALTELE AF+SLAGDQDFQKELD
Sbjct: 61  SLAMEDKLHTLSLQQRPDSFGRFGRFGGKYVPETLMHALTELEAAFYSLAGDQDFQKELD 120

Query: 121 GILRDYVGRESPLYFAERLTEHYRRSNGEGPHIFLKREDLNHTGAHKINNAVAQALLAKR 180
           GILRDYVGRESPLYFAERLTEHYRRSNGEGPHIFLKREDLNHTGAHKINNAVAQALLAKR
Sbjct: 121 GILRDYVGRESPLYFAERLTEHYRRSNGEGPHIFLKREDLNHTGAHKINNAVAQALLAKR 180

Query: 181 LGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMRLLGAEVRPVH 240
           LGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMRLLGAEVRPVH
Sbjct: 181 LGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMRLLGAEVRPVH 240

Query: 241 SGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKW 300
           SGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKW
Sbjct: 241 SGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKW 300

Query: 301 GGKPDVLVACVGGGSNAMGLFHEFVNDKDVRLVGVEAAGFGLDSGKHAATLTKGEVGVLH 360
           GGKPDVLVACVGGGSNAMGLFHEFVND+DVRLVGVEAAGFGLDSGKHAATLTKGEVGVLH
Sbjct: 301 GGKPDVLVACVGGGSNAMGLFHEFVNDEDVRLVGVEAAGFGLDSGKHAATLTKGEVGVLH 360

Query: 361 GAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSYLKDLGRAEYHSVTDDEALEAFKRLS 420
           GAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSYLKDLGRAEYHSVTDDEALEAFKRLS
Sbjct: 361 GAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSYLKDLGRAEYHSVTDDEALEAFKRLS 420

Query: 421 RLEGIIPALETSHALAYLEKLCLTLPDGTKVVLNCSGRGDKDVQTAIKYLQV 472
           RLEGIIPALETSHALAYLEKLC TLPDGTKVVLNCSGRGDKDVQTAIKYLQV
Sbjct: 421 RLEGIIPALETSHALAYLEKLCPTLPDGTKVVLNCSGRGDKDVQTAIKYLQV 472

BLAST of Chy2G039530 vs. NCBI nr
Match: KAA0053097.1 (tryptophan synthase beta chain 1 [Cucumis melo var. makuwa] >TYK29942.1 tryptophan synthase beta chain 1 [Cucumis melo var. makuwa])

HSP 1 Score: 911 bits (2354), Expect = 0.0
Identity = 459/483 (95.03%), Postives = 464/483 (96.07%), Query Frame = 0

Query: 1   MVASTALNCKIPTFSPSSSHSNNKISSSISHFPVRFRRVSSNPSPVFRSNAVSCTLTREP 60
           MVASTALNCKIPT SPS SHSNNKISSSI HFPVRFRRVSSNPSP+ RSNAVSCTLTREP
Sbjct: 1   MVASTALNCKIPTISPSPSHSNNKISSSIPHFPVRFRRVSSNPSPLLRSNAVSCTLTREP 60

Query: 61  SLAMEDKVNNSSLQQRPDSFGRFGRFGGKYVPETLMHALTELETAFFSLAGDQDFQKELD 120
           SLAMEDK++  SLQQRPDSFGRFGRFGGKYVPETLMHALTELE AF+SLAGDQDFQKELD
Sbjct: 61  SLAMEDKLHTLSLQQRPDSFGRFGRFGGKYVPETLMHALTELEAAFYSLAGDQDFQKELD 120

Query: 121 GILRDYVGRESPLYFAERLTEHYRRSNGEGPHIFLKREDLNHTGAHKINNAVAQALLAKR 180
           GILRDYVGRESPLYFAERLTEHYRRSNGEGPHIFLKREDLNHTGAHKINNAVAQALLAKR
Sbjct: 121 GILRDYVGRESPLYFAERLTEHYRRSNGEGPHIFLKREDLNHTGAHKINNAVAQALLAKR 180

Query: 181 LGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMRLLGAEVRPVH 240
           LGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMRLLGAEVRPVH
Sbjct: 181 LGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMRLLGAEVRPVH 240

Query: 241 SGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKW 300
           SGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKW
Sbjct: 241 SGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKW 300

Query: 301 GGKPDVLVACVGGGSNAMGLFHEFVNDKDVRLVGVEAAGFGLDSGKHAATLTKGEVGVLH 360
           GGKPDVLVACVGGGSNAMGLFHEFVND+DVRLVGVEAAGFGLDSGKHAATLTKGEVGVLH
Sbjct: 301 GGKPDVLVACVGGGSNAMGLFHEFVNDEDVRLVGVEAAGFGLDSGKHAATLTKGEVGVLH 360

Query: 361 GAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSYLKDLGRAEYHSVTDDEALE------ 420
           GAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSYLKDLGRAEYHSVTDDEALE      
Sbjct: 361 GAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSYLKDLGRAEYHSVTDDEALEEGKFII 420

Query: 421 -----AFKRLSRLEGIIPALETSHALAYLEKLCLTLPDGTKVVLNCSGRGDKDVQTAIKY 472
                AFKRLSRLEGIIPALETSHALAYLEKLC TLPDGTKVVLNCSGRGDKDVQTAIKY
Sbjct: 421 WLVTAAFKRLSRLEGIIPALETSHALAYLEKLCPTLPDGTKVVLNCSGRGDKDVQTAIKY 480

BLAST of Chy2G039530 vs. NCBI nr
Match: XP_038899607.1 (tryptophan synthase beta chain 1 [Benincasa hispida])

HSP 1 Score: 902 bits (2330), Expect = 0.0
Identity = 450/472 (95.34%), Postives = 459/472 (97.25%), Query Frame = 0

Query: 1   MVASTALNCKIPTFSPSSSHSNNKISSSISHFPVRFRRVSSNPSPVFRSNAVSCTLTREP 60
           MVASTALNCKIPT SPSSSHSN KISSS S FPV FRRV SNPSPVFRSNA+SCTLTREP
Sbjct: 1   MVASTALNCKIPTISPSSSHSNTKISSSNSRFPVHFRRVLSNPSPVFRSNAISCTLTREP 60

Query: 61  SLAMEDKVNNSSLQQRPDSFGRFGRFGGKYVPETLMHALTELETAFFSLAGDQDFQKELD 120
           SLAMEDK+++ +L+QRPDSFG FGRFGGKYVPETLMHALTELETAF SLAGDQ+FQKELD
Sbjct: 61  SLAMEDKLHDLNLEQRPDSFGHFGRFGGKYVPETLMHALTELETAFHSLAGDQEFQKELD 120

Query: 121 GILRDYVGRESPLYFAERLTEHYRRSNGEGPHIFLKREDLNHTGAHKINNAVAQALLAKR 180
           GILRDYVGRESPLYFAERLTEHYRR NGEGPHIFLKREDLNHTGAHKINNAVAQALLAKR
Sbjct: 121 GILRDYVGRESPLYFAERLTEHYRRPNGEGPHIFLKREDLNHTGAHKINNAVAQALLAKR 180

Query: 181 LGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMRLLGAEVRPVH 240
           LGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDM+RQALNVFRMRLLGAEVRPVH
Sbjct: 181 LGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMDRQALNVFRMRLLGAEVRPVH 240

Query: 241 SGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKW 300
           SGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKW
Sbjct: 241 SGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKW 300

Query: 301 GGKPDVLVACVGGGSNAMGLFHEFVNDKDVRLVGVEAAGFGLDSGKHAATLTKGEVGVLH 360
           GG PDVLVACVGGGSNAMGLFHEFVND+DVRLVGVEAAGFGLDSGKHAATLTKGEVGVLH
Sbjct: 301 GGTPDVLVACVGGGSNAMGLFHEFVNDEDVRLVGVEAAGFGLDSGKHAATLTKGEVGVLH 360

Query: 361 GAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSYLKDLGRAEYHSVTDDEALEAFKRLS 420
           GAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSYLKDLGRAEYHSVTDDEALEAFKRLS
Sbjct: 361 GAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSYLKDLGRAEYHSVTDDEALEAFKRLS 420

Query: 421 RLEGIIPALETSHALAYLEKLCLTLPDGTKVVLNCSGRGDKDVQTAIKYLQV 472
           RLEGIIPALETSHALAYLEKLC TLPDG KVVLNCSGRGDKDVQTAIKYLQV
Sbjct: 421 RLEGIIPALETSHALAYLEKLCPTLPDGAKVVLNCSGRGDKDVQTAIKYLQV 472

BLAST of Chy2G039530 vs. NCBI nr
Match: XP_022139544.1 (tryptophan synthase beta chain 1 [Momordica charantia])

HSP 1 Score: 868 bits (2242), Expect = 0.0
Identity = 436/472 (92.37%), Postives = 446/472 (94.49%), Query Frame = 0

Query: 1   MVASTALNCKIPTFSPSSSHSNNKISSSISHFPVRFRRVSSNPSPVFRSNAVSCTLTREP 60
           MVA TALNCKIPT SPSSSHSN++ISSS S  P+RFRR +S PSP  R   VSCTLT +P
Sbjct: 1   MVAFTALNCKIPTVSPSSSHSNSEISSSFSRLPLRFRRAASYPSPAARRCGVSCTLTTQP 60

Query: 61  SLAMEDKVNNSSLQQRPDSFGRFGRFGGKYVPETLMHALTELETAFFSLAGDQDFQKELD 120
           SLAMEDK       QRPDSFGRFGRFGGKYVPETLMHALT+LE AF SLAGDQ+FQKELD
Sbjct: 61  SLAMEDKTQT----QRPDSFGRFGRFGGKYVPETLMHALTQLEAAFHSLAGDQEFQKELD 120

Query: 121 GILRDYVGRESPLYFAERLTEHYRRSNGEGPHIFLKREDLNHTGAHKINNAVAQALLAKR 180
           GILRDYVGRESPLYFAERLTEHYRR NGEGPHIFLKREDLNHTGAHKINNAVAQALLAKR
Sbjct: 121 GILRDYVGRESPLYFAERLTEHYRRPNGEGPHIFLKREDLNHTGAHKINNAVAQALLAKR 180

Query: 181 LGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMRLLGAEVRPVH 240
           LGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMRLLGAEV+PVH
Sbjct: 181 LGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMRLLGAEVKPVH 240

Query: 241 SGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKW 300
           SGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKW
Sbjct: 241 SGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKW 300

Query: 301 GGKPDVLVACVGGGSNAMGLFHEFVNDKDVRLVGVEAAGFGLDSGKHAATLTKGEVGVLH 360
           GGKPDVLVACVGGGSNAMGLFHEFV DKDVRLVGVEAAGFGLDSGKHAATLTKGE+GVLH
Sbjct: 301 GGKPDVLVACVGGGSNAMGLFHEFVGDKDVRLVGVEAAGFGLDSGKHAATLTKGEIGVLH 360

Query: 361 GAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSYLKDLGRAEYHSVTDDEALEAFKRLS 420
           GAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSYLKDLGRAEY+SVTDDEALEAFKRLS
Sbjct: 361 GAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSYLKDLGRAEYYSVTDDEALEAFKRLS 420

Query: 421 RLEGIIPALETSHALAYLEKLCLTLPDGTKVVLNCSGRGDKDVQTAIKYLQV 472
           RLEGIIPALETSHALAYLEKLC TLPDGTKVVLNCSGRGDKDV TAIKYLQV
Sbjct: 421 RLEGIIPALETSHALAYLEKLCPTLPDGTKVVLNCSGRGDKDVHTAIKYLQV 468

BLAST of Chy2G039530 vs. TAIR 10
Match: AT5G54810.1 (tryptophan synthase beta-subunit 1 )

HSP 1 Score: 769.2 bits (1985), Expect = 1.9e-222
Identity = 385/464 (82.97%), Postives = 417/464 (89.87%), Query Frame = 0

Query: 13  TFSPSSSHSNNKISSSISHFPVRFRRVSSNPSPVFRSN----AVSCTLTREPSLAMEDKV 72
           TF  S S + +  SS ++H    F+ V   P P  RS     +VSCT+ ++P + M    
Sbjct: 9   TFRASVSSAPSS-SSQLTHLKSPFKAVKYTPLPSSRSKSSSFSVSCTIAKDPPVLMA-AG 68

Query: 73  NNSSLQQRPDSFGRFGRFGGKYVPETLMHALTELETAFFSLAGDQDFQKELDGILRDYVG 132
           ++ +L QRPDSFGRFG+FGGKYVPETLMHAL+ELE+AF++LA D DFQ+EL GIL+DYVG
Sbjct: 69  SDPALWQRPDSFGRFGKFGGKYVPETLMHALSELESAFYALATDDDFQRELAGILKDYVG 128

Query: 133 RESPLYFAERLTEHYRRSNGEGPHIFLKREDLNHTGAHKINNAVAQALLAKRLGKKRIIA 192
           RESPLYFAERLTEHYRR NGEGP I+LKREDLNHTGAHKINNAVAQALLAKRLGKKRIIA
Sbjct: 129 RESPLYFAERLTEHYRRENGEGPLIYLKREDLNHTGAHKINNAVAQALLAKRLGKKRIIA 188

Query: 193 ETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMRLLGAEVRPVHSGTATLKD 252
           ETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMRLLGAEVR VHSGTATLKD
Sbjct: 189 ETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMRLLGAEVRGVHSGTATLKD 248

Query: 253 ATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKWGGKPDVLV 312
           ATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKWGGKPDVLV
Sbjct: 249 ATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKWGGKPDVLV 308

Query: 313 ACVGGGSNAMGLFHEFVNDKDVRLVGVEAAGFGLDSGKHAATLTKGEVGVLHGAMSYLLQ 372
           ACVGGGSNAMGLFHEFVND +VR++GVEAAGFGLDSGKHAATLTKG+VGVLHGAMSYLLQ
Sbjct: 309 ACVGGGSNAMGLFHEFVNDTEVRMIGVEAAGFGLDSGKHAATLTKGDVGVLHGAMSYLLQ 368

Query: 373 DDDGQIIEPHSISAGLDYPGVGPEHSYLKDLGRAEYHSVTDDEALEAFKRLSRLEGIIPA 432
           DDDGQIIEPHSISAGLDYPGVGPEHS+ KD+GRAEY+S+TD+EALEAFKR+SRLEGIIPA
Sbjct: 369 DDDGQIIEPHSISAGLDYPGVGPEHSFFKDMGRAEYYSITDEEALEAFKRVSRLEGIIPA 428

Query: 433 LETSHALAYLEKLCLTLPDGTKVVLNCSGRGDKDVQTAIKYLQV 473
           LETSHALAYLEKLC TL DGT+VVLN SGRGDKDVQT  KYL V
Sbjct: 429 LETSHALAYLEKLCPTLSDGTRVVLNFSGRGDKDVQTVAKYLDV 470

BLAST of Chy2G039530 vs. TAIR 10
Match: AT4G27070.1 (tryptophan synthase beta-subunit 2 )

HSP 1 Score: 768.1 bits (1982), Expect = 4.2e-222
Identity = 384/469 (81.88%), Postives = 425/469 (90.62%), Query Frame = 0

Query: 13  TFSPSSSHSNNKISSSISHFPVRFRRVSSNPSPVFRSN---AVSCTLTREPSLAMEDKV- 72
           TF PSS  ++++++   S  P +  + +  PS   RS+   +VSCT+ ++P++ M D   
Sbjct: 9   TFRPSSVSASSELTHLRS--PSKLPKFTPLPSARSRSSSSFSVSCTIAKDPAVVMADSEK 68

Query: 73  -----NNSSLQQRPDSFGRFGRFGGKYVPETLMHALTELETAFFSLAGDQDFQKELDGIL 132
                ++ ++ QRPDSFGRFG+FGGKYVPETLMHAL+ELETAF+SLA D+DFQ+EL  IL
Sbjct: 69  IKAAGSDPTMWQRPDSFGRFGKFGGKYVPETLMHALSELETAFYSLATDEDFQRELAEIL 128

Query: 133 RDYVGRESPLYFAERLTEHYRRSNGEGPHIFLKREDLNHTGAHKINNAVAQALLAKRLGK 192
           +DYVGRESPLYFAERLTEHYRR NGEGP I+LKREDLNHTGAHKINNAVAQALLAKRLGK
Sbjct: 129 KDYVGRESPLYFAERLTEHYRRENGEGPLIYLKREDLNHTGAHKINNAVAQALLAKRLGK 188

Query: 193 KRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMRLLGAEVRPVHSGT 252
           KRIIAETGAGQHGVATATVCARFGL+CIIYMGAQDMERQALNVFRMRLLGAEVR VHSGT
Sbjct: 189 KRIIAETGAGQHGVATATVCARFGLQCIIYMGAQDMERQALNVFRMRLLGAEVRGVHSGT 248

Query: 253 ATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKWGGK 312
           ATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQA+EKWGGK
Sbjct: 249 ATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQAMEKWGGK 308

Query: 313 PDVLVACVGGGSNAMGLFHEFVNDKDVRLVGVEAAGFGLDSGKHAATLTKGEVGVLHGAM 372
           PDVLVACVGGGSNAMGLFHEFV+D +VR++GVEAAGFGLDSGKHAATLTKG+VGVLHGAM
Sbjct: 309 PDVLVACVGGGSNAMGLFHEFVDDTEVRMIGVEAAGFGLDSGKHAATLTKGDVGVLHGAM 368

Query: 373 SYLLQDDDGQIIEPHSISAGLDYPGVGPEHSYLKDLGRAEYHSVTDDEALEAFKRLSRLE 432
           SYLLQDDDGQIIEPHSISAGLDYPGVGPEHS+LKD+GRAEY SVTD+EALEAFKR+SRLE
Sbjct: 369 SYLLQDDDGQIIEPHSISAGLDYPGVGPEHSFLKDVGRAEYFSVTDEEALEAFKRVSRLE 428

Query: 433 GIIPALETSHALAYLEKLCLTLPDGTKVVLNCSGRGDKDVQTAIKYLQV 473
           GIIPALETSHALA+LEKLC TLPDG +VVLN SGRGDKDVQTAIKYL+V
Sbjct: 429 GIIPALETSHALAHLEKLCPTLPDGARVVLNFSGRGDKDVQTAIKYLEV 475

BLAST of Chy2G039530 vs. TAIR 10
Match: AT5G28237.1 (Pyridoxal-5'-phosphate-dependent enzyme family protein )

HSP 1 Score: 543.1 bits (1398), Expect = 2.2e-154
Identity = 261/396 (65.91%), Postives = 322/396 (81.31%), Query Frame = 0

Query: 76  RPDSFGRFGRFGGKYVPETLMHALTELETAFFSLAGDQDFQKELDGILRDYVGRESPLYF 135
           R D  G+FGRFGGK+VPETLM  L ELE  F  +  D +FQ+EL   LRDYVGRE+PLYF
Sbjct: 65  RGDGNGKFGRFGGKFVPETLMSRLIELEDEFNFVRCDHEFQEELTTALRDYVGRETPLYF 124

Query: 136 AERLTEHYRR---SNGEGPHIFLKREDLNHTGAHKINNAVAQALLAKRLGKKRIIAETGA 195
           AERLTEHY+    +   GP I+LKREDL+H G+HKINNA+AQA++++RLG  R++A TGA
Sbjct: 125 AERLTEHYKNIVPTIEGGPEIYLKREDLSHCGSHKINNALAQAMISRRLGCSRVVAATGA 184

Query: 196 GQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMRLLGAEVRPVHSGTATLKDATSE 255
           GQHGVATA  CA+  LEC ++MGA D+E+Q+ NV  M+LLGA+V  V     T KDA+SE
Sbjct: 185 GQHGVATAAACAKLSLECTVFMGAADIEKQSFNVLSMKLLGAQVISVE---GTFKDASSE 244

Query: 256 AIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKWGGKPDVLVACVG 315
           AIR+WV N+ TT+Y+ G+V GPHP P++VR+F +VIGKETR+QA + WGGKPDVLVACVG
Sbjct: 245 AIRNWVENLYTTYYLSGTVVGPHPCPIIVREFQSVIGKETRRQAKQLWGGKPDVLVACVG 304

Query: 316 GGSNAMGLFHEFVNDKDVRLVGVEAAGFGLDSGKHAATLTKGEVGVLHGAMSYLLQDDDG 375
            GSNA+GLFHEFV D+DVRLVGVEAAG GLDSGKH+ATL  G+VGV HG+MSYLLQDD G
Sbjct: 305 SGSNALGLFHEFVGDEDVRLVGVEAAGLGLDSGKHSATLAFGDVGVYHGSMSYLLQDDQG 364

Query: 376 QIIEPHSISAGLDYPGVGPEHSYLKDLGRAEYHSVTDDEALEAFKRLSRLEGIIPALETS 435
           QI++PHS+  GL+YPGVGPE S++K+ GRAE+++ TD+EA++A  RLSRLEGIIPALE S
Sbjct: 365 QILKPHSVGVGLEYPGVGPEISFMKETGRAEFYTATDEEAIQACMRLSRLEGIIPALEAS 424

Query: 436 HALAYLEKLCLTLPDGTKVVLNCSGRGDKDVQTAIK 469
           HALA+L+KL  TL DG KVV+NCSGRGDKD+ T I+
Sbjct: 425 HALAFLDKLVPTLRDGAKVVVNCSGRGDKDLDTLIQ 457

BLAST of Chy2G039530 vs. TAIR 10
Match: AT5G38530.1 (tryptophan synthase beta type 2 )

HSP 1 Score: 125.9 bits (315), Expect = 8.4e-29
Identity = 110/366 (30.05%), Postives = 161/366 (43.99%), Query Frame = 0

Query: 129 RESPLYFAERLTEHYRRSNGEGPHIFLKREDLNHTGAHKINNAVAQALLAKRLGKKRIIA 188
           R +PL  A+RL +  +        I+ K E  +  G+HK N AV QA    + G K ++ 
Sbjct: 132 RPTPLIRAKRLEKLLQTP----ARIYFKYEGGSPAGSHKPNTAVPQAYYNAKEGVKNVVT 191

Query: 189 ETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMRLLGAEVRPV--------- 248
           ETGAGQ G + A   + FGL+C ++  A     +      M+  GA+V P          
Sbjct: 192 ETGAGQWGSSLAFASSLFGLDCEVWQVANSYHTKPYRRLMMQTWGAKVHPSPSDLTEAGR 251

Query: 249 ------HSGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETR 308
                  S   +L  A SEA+     N E T Y LGSV         V     +IG+E  
Sbjct: 252 RILESDPSSPGSLGIAISEAVEVAARN-EDTKYCLGSVLN------HVLLHQTIIGEECI 311

Query: 309 KQALEKWGGKPDVLVACVGGGSNAMGLFHEFVNDKDVRLVGVEAAGFGLDSGKHAATLTK 368
           +Q +E +G  PD+++ C GGGSN  GL   F+ +K   L G               +LTK
Sbjct: 312 QQ-MENFGETPDLIIGCTGGGSNFAGLSFPFIREK---LKGKINPVIRAVEPSACPSLTK 371

Query: 369 G----EVGVLHGAMSYLLQDDDGQIIEPHSISA-GLDYPGVGPEHSYLKDLGRAEYHSVT 428
           G    + G   G    +     G    P  I A GL Y G+ P  S++ + G  E  S+ 
Sbjct: 372 GVYAYDFGDTAGLTPLMKMHTLGHDFIPDPIHAGGLRYHGMAPLISHVYEQGFMEAISIP 431

Query: 429 DDEALEAFKRLSRLEGIIPALETSHALAYLEK---LCLTLPDGTKVVLNCSGRGDKDVQT 472
             E  +   + +R EGIIPA E +HA+A   +    C    +   +++   G G  D+ +
Sbjct: 432 QIECFQGAIQFARTEGIIPAPEPTHAIAATIREALRCKETGEAKVILMAMCGHGHFDLTS 482

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P146712.6e-22182.97Tryptophan synthase beta chain 1, chloroplastic OS=Arabidopsis thaliana OX=3702 ... [more]
P252695.9e-22181.88Tryptophan synthase beta chain 2, chloroplastic OS=Arabidopsis thaliana OX=3702 ... [more]
O500469.4e-21981.76Tryptophan synthase beta chain 2, chloroplastic OS=Camptotheca acuminata OX=1692... [more]
P432845.2e-20983.76Tryptophan synthase beta chain 2, chloroplastic (Fragment) OS=Zea mays OX=4577 G... [more]
P432831.3e-20488.43Tryptophan synthase beta chain 1 (Fragment) OS=Zea mays OX=4577 GN=TSB1 PE=2 SV=... [more]
Match NameE-valueIdentityDescription
A0A0A0M0659.3e-27098.52Tryptophan synthase OS=Cucumis sativus OX=3659 GN=Csa_1G660140 PE=3 SV=1[more]
A0A1S3C0164.8e-26697.25Tryptophan synthase OS=Cucumis melo OX=3656 GN=LOC103494920 PE=3 SV=1[more]
A0A5D3E2251.7e-26395.03Tryptophan synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold588G... [more]
A0A6J1CDB21.3e-25092.37Tryptophan synthase OS=Momordica charantia OX=3673 GN=LOC111010430 PE=3 SV=1[more]
A0A6J1J2613.4e-24891.95Tryptophan synthase OS=Cucurbita maxima OX=3661 GN=LOC111480575 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_004142558.10.098.52tryptophan synthase beta chain 1 [Cucumis sativus] >KGN66694.1 hypothetical prot... [more]
XP_008454527.10.097.25PREDICTED: tryptophan synthase beta chain 1 [Cucumis melo][more]
KAA0053097.10.095.03tryptophan synthase beta chain 1 [Cucumis melo var. makuwa] >TYK29942.1 tryptoph... [more]
XP_038899607.10.095.34tryptophan synthase beta chain 1 [Benincasa hispida][more]
XP_022139544.10.092.37tryptophan synthase beta chain 1 [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT5G54810.11.9e-22282.97tryptophan synthase beta-subunit 1 [more]
AT4G27070.14.2e-22281.88tryptophan synthase beta-subunit 2 [more]
AT5G28237.12.2e-15465.91Pyridoxal-5'-phosphate-dependent enzyme family protein [more]
AT5G38530.18.4e-2930.05tryptophan synthase beta type 2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006654Tryptophan synthase, beta chainTIGRFAMTIGR00263TIGR00263coord: 81..469
e-value: 2.3E-186
score: 617.4
IPR006654Tryptophan synthase, beta chainCDDcd06446Trp-synth_Bcoord: 98..465
e-value: 0.0
score: 690.043
IPR036052Tryptophan synthase beta subunit-like PLP-dependent enzymeGENE3D3.40.50.1100coord: 128..463
e-value: 6.8E-198
score: 659.0
IPR036052Tryptophan synthase beta subunit-like PLP-dependent enzymeGENE3D3.40.50.1100coord: 83..285
e-value: 6.8E-198
score: 659.0
IPR036052Tryptophan synthase beta subunit-like PLP-dependent enzymeSUPERFAMILY53686Tryptophan synthase beta subunit-like PLP-dependent enzymescoord: 77..470
IPR023026Tryptophan synthase beta chain/beta chain-likePIRSFPIRSF001413Trp_syn_betacoord: 64..472
e-value: 2.9E-164
score: 545.0
IPR023026Tryptophan synthase beta chain/beta chain-likeHAMAPMF_00133Trp_synth_betacoord: 76..470
score: 53.01189
IPR001926Pyridoxal-phosphate dependent enzymePFAMPF00291PALPcoord: 128..457
e-value: 7.3E-47
score: 160.2
NoneNo IPR availablePIRSRPIRSR006278-2PIRSR006278-2coord: 151..337
e-value: 1.5E-9
score: 35.7
NoneNo IPR availablePIRSRPIRSR605856-51PIRSR605856-51coord: 147..338
e-value: 1.7E-6
score: 25.3
NoneNo IPR availablePANTHERPTHR48077TRYPTOPHAN SYNTHASE-RELATEDcoord: 23..472
NoneNo IPR availablePANTHERPTHR48077:SF8TRYPTOPHAN SYNTHASE BETA CHAIN 1, CHLOROPLASTIC-RELATEDcoord: 23..472
IPR006653Tryptophan synthase, beta chain, conserved sitePROSITEPS00168TRP_SYNTHASE_BETAcoord: 160..174

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy2G039530.1Chy2G039530.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000162 tryptophan biosynthetic process
biological_process GO:0006568 tryptophan metabolic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0004834 tryptophan synthase activity