Chy2G038770 (gene) Cucumber (hystrix) v1

Overview
NameChy2G038770
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionBeta-amyrin 28-monooxygenase
LocationchrH02: 14962422 .. 14970327 (+)
RNA-Seq ExpressionChy2G038770
SyntenyChy2G038770
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGCTTTTCCTCATTTCTCTCTTAATCCTTTTGTTCTTCTTTCTTTCTCTTACTCTCTTCATCCTCTTCCACAATCACAAATCCTTATTCTCTTATCCCAACACTCCTCCCGGCGCCATCGGCCTTCCCATACTCGGCGAGAGCCTCGAGTTCTTATCATCTGGTTGGAGAGGCCATCCTGAGAAGTTCATCTTCGATCGTTTGAATAAGTACAAGTCAGATGTGTTCAAAACCTCGATCGTGGGCGTTCCAGCCGCCATTTTCTGCGGCCCTATTTGTAACAAGTTCCTCTTCTCTAACGAGAATAAACTGGTTACTCCTTGGTGGCCAGATTCCGTGAACAAGATCTTCCCCTCTACAACGCAGACTAGCTCCAAAGAAGAAGCTAAGAAACTCAAGAAACTCCTTCCGCAATTCCTTAAACCCGAAGCGCTTCAGCGTTATATTGGAATTATGGACGAACTTGCTGAACGCCATTTCAATTCCTTTTGGGAGAACAGAGAAGAGGTCCTCGTGTTTCCTCTTGCTAAAAGGTAAATGATTTATCTTATCTTTGATTTCTTATACGAATAAAATTGGTGAGGAATCAAATTTTATTAAAATCACAGTAAAACTGAATGATTTACAATACCTCACTCATGAATAAATACTACTTGAAATATAAGTGGACGTAACAAAAAAGATAGTGAAAAACTGGGTTCAAAGTAAATCCACAACAAACTCTAACTTAATGCTAATGAGTTACAACAAACTTCAATAATTTCATCATACCCCTGAAGTTGAGCATGAATATAAAAATTGTCCAACTTTGCATAAAATTGAGGATAAAGCCGAAGAGGTTAACGCTTTTGTAAACATGTCTGCAAGTTGTGCTGAAGAACGAACAGATAATAATTGTTTTAAGAAACTGTCAACGATTTTATCACGAACAAAATGACAATCGATCTCGATGTGTTTGTTCATTAATGAAATGTTGGATTGGAAGAAATAGATATAGCTGCTTGTTTGTCACAATAGAAGACAATGGGAAGCAAAACTGAAACATTCAAATCAAGAAGAATTTAGATTAATCCAGATGATTTAACTAGTAAGAGAAGCCCAAGCTCGATACTCAGCTTCAGCAGAAGATCGGGACATTGTCGTCACCTAAAAATATAATGCAAAAGCTAGTGATTGAACGTCGTGTGTCAAGACACAGTTCAGAATCAGCATCAACAAAGGCCCGAATTTGAAATGAGGAAACTGATTTAAATAAAATGCCTTGTCCAAGTGCTCTCTTTAGATAAAGTAGCAAATGATGGACAGCTTGTAAATGAGACTGTGTGGGAGCTTGTAAGAATTGACTCAAATGATGAACCGCAAAACAAATATCAGGACGTGAGATTTGAAGATACAATAACCGACCAATTACTAATCAAAAGCTAATTCCAAACGAATGTTCTGTGAATCTGAAGCGAACTCAGAAAATTCACCATCAAAAAGCTTCTATCCCTTTGCATCAGCCGGATGTCTCAACACATCCTCTGTTTTGACTGGTTTATCCTTATATCATCTCATGTCAGACGAGTCCTCTTGCAATACAAACAATCGCTATAATCTTGGTATCAACGAAAAGTGTTGCAATACCTCACACCGAGTTCTTTTCCCTCTATTAGAACTAACCTTGTACTGAGACTCAACATAAGTTGGACAATGCTGCAAATCCCCAAACGCCTGCAAATCCCCAAACGCCTTCTAATATCTCTAACAACATGTTCTCCAACCGTTGAGAAACTTTACATGTATCAACGTAACCAAAAAGTTTACGGATGAAAATTCTAAATAGCCGGGTATAGATCAGTACGTGCTTGGTTTAATAACTCCTGAAATATGTTTGTTGTCTCTTATCCTACACCACAATTAAATGAAATGACATTCTCTAAACCTTGCTCTATTTCTTCTTCGTGTTCAATCGAAACTCGTAAATTATGAAGCATACCCAACATTTCATTGTCTTTAGGAAAAGGTTTACTGCTAGTTTCTTCATGAAAGGGGCCAGTACTGGTTCCTTCATGGAAAGGGTCGTCACTGCTAGTTCCTTCATCAAATCTTTCTATATCTCTATGCAAGTTCACTAGCTCTCCATGATACAACCAATCTCGTTAATAAATGTCGCTCCACACCCTCTAATGAGTCTCAATTTGAGTTCATACATCTCTTGCATGGACACCTTGTCCGTCCGTACTCATCGACGTGATACTTCAAAACCTCTGAAAATTTGGACACTCCCTCTCTATACTCAATCGAAAACTTATTTCTAAGTTTCATCCAACCCCTGTCCATCGTTAAAGACCTAGAACATAAATAGGTTTCATTAGTCTTATATTTCTCCTCTCAAATGCTCAAACTTACTCTATTGATAAGCTATACCTCACGAGTATATCCAATATCCCAAGTTTCACTACATCTAAGTTTAAACTAAAAAAGCTACCTAACACTTCCAACTAACAAGCTTTAGAAGCTACCTAACACTTCCAACAAATTATAAACCACTAAAGGCTACGACAATTCGAACAAACACTTAATTCTAACAAATAAAACACATAGTCACAAATAAAAAACTATAGACTACCATAACTACGGATAGCCACAACAAATCAATAAAAATTTTCAAGTATTTAAACTATTTCAACAATTTACAAATTTTCAATTCATCAATTCAATAATTTCAATATATTTAAATAAATTTCAACTGGACCCCTCTATAACAATCACAATTTCAATAATTTAAACCCTAACTATAACAAGTTTCAATATTTCATTTATACATACAATTTAATTTATAAGCGATTCATATACACTTTAATTCATAAACTTCCAACAATTTCAGTATACAATCTATACATACAATTTAATATCAATCATACATTTATTTATTCATGCAACAAATTTAATATCAATGTTACATACAATATACAATCTACCATATAATATAAATACAATATACAATATACAATCTACAACAGTGGAGGATCCAAAAAATTTATTGACAAGGGAGACTTCAAAAAAGATAATGGCCCCATTGATTTTCTTTTAAAATATTTCTCCATTTAGATTTTAAATTAAATGTAAATAACATAACATTGTAATGAGAATAAATCAAGAAGATATTGACATATAACAACAATATCATAACTTAAACAATGCAACAAGTTTAATTAAGATCCATAAATTATTTCAAGAATTAATAAAATAGGTATAAAGCAATTGAACTAATACAAAAAAATAAGCTAAGAATGATTTGAAATGCATAAAAATAAAATAACAAACTTGAAAAGAAAAGAAAATAAATTACAAAACTTACCTCATAAATTAGGAGATAAAGTTGAAGAATAACCCAGAAGAAGTTGAAGAAGAAATTTTAAAAACTAAAACAAAAAATAAGAGTGATTTTAAATATTAAAACGAATGCATGTACGTAAAATAACAAACTTAAAAAAAATAAAAGGAAATAAATAAAAATTTACCTCATCAACTTTGAGAAGATATTGAAGAAGAAAAATAGTTGAAAGAAAAGATGTTAGAAGTTTTATAAAAAGAAAATATGAATGAAGTTGAATAATTTTAATTTAGGTGGGGAAGAGTGTAGTTGAAAAAAAAAAGATGTTGAAGTTTTATAAAAATAAAAAAACGAATGAAGTGAAGTTGAATGACTTTAATTTAGGTGGGAAAGAGTGTAGAATTATTATTTCTAGAAAGATTGTAATTTGAGGAACCTGGAATGGAAGGAGGGCTGACAAACAGAGTAACCACTAAGCTATTCTTTATTTATATTATATAAACACGATAAATTTGAGGGGGGGCCAGAGCCCCTGGGGCCGATGATCTACAATCTATACATACAATATACAATATACAATATATCAATCATACATTTATTTAGAAACAAAAAAAAAAAGTATTTTTACAGGAATGTAGAGACAACTTAATACGACGACGAAAGGAGGATGATGGGGGGTGGAACACGACGACGACGGGCAAGGAGACGGCAAACGACGATGGCATAAACTCTCTCTCTCTGGTTGTCTAACTCTCTCTCTTTTCTCTTTCGTGTGTTCTGTGTTTTTGGAACACAAAACACTTATTAACCGGGGTTTTCCCAATGCAGCTCAAAAACGTCGCGATAACCCCCTAATCCAGATGTCATAAGTGAAAACATCGAGATTACTTGGGCTCAATCTCGACGTTTCACTTATGGCGTAGAGGAAAAGTTTTACAAATTATTCAAACGTTATAGTTTCTCCCGACGTGGTCAACCTTTATGTCTGCCATAGTCTGACCAATCCCAAAGTCTCACTTACTGCGTCAGGGAAAGGTTGAAATAATTATTTAAAGGTTAAATTTCCCTGTCGTGCTTAACGCTAATGCCGTAGAGTCTTACTAATTTCTGACGCCATGCACATAAACATCGGGGTAATCATAAATTTTAATAATAATTTTCATTTTTCATGCCGTATTAATAATAATTTCCATTTTTCCCGACGTATTAATAATAATGTTCATTTTCCCCGACGTCATACTGGGGCTTCCCGACGTGTACTTTCATGGCATCGGGGAAAATATATATTATTATTTAAATAAAATGTTTCCCCGATGTTTTCCTACGGTAAGTGATGCGGTCCTGACGTCTTCAACACTAAGTCAAGACTCCGGCGACTGTTGACACAGATTTTCTAGCGCCGGAGAAAGTATTGTTTCCCGACTTTGTCTATATCGAAAGCATTGTTTCCCAACTTTGTCTATGTCGAAAGCATTGTTTCCCGACTTTGTCTATGCCGACACCTTTTTTACATTGGGATAGCTCCGTTTTCTTGTATATAGTGAGGTCACCCAACATAGAATTGATCATTCTTAACATTGGAGTACATTGGAGTTCGGATGATCAAGCCACCGAAAATGAAGGATTCTTTTAAATCTTTCTTAATCCCTCTCATTTAGATGTGCGTCACAAGTAGTTAACTTAAGTTTTGTACTCGTTAAAATTGCAAGTTCAGCATGATCACGCATAAGCGTTTAGTTATGTTTAAAAGAAGTAAACTAGGCGTAGGCGAACGCCCATGTCATGTCAATACTTGGTAAGTAGAAAGTGTGTCCTTGAATCCACAATTTACACAAATAGGAGGAGAACATTTTGTAACGATCCTTATTTCCTTTTTTATTAGGAAAACTTGAAAAATAGTAAAAAAACATTATGATACCAGAGCCCATGACATACAAATTTCAAAATATTTTTGTCTGATGCAATTTCCGTTTCTATTATCGTAGCAGCAAGAAATGATGGATCTCCAATATTTCTTTGATTTTCTTCTTGGATGAGGAGTAAAAAAACTTTAGTAATGGATGGCAAGGGATCCATCAAAAGAATTTGTGCGCGAGTGCTTGCATAAGTCTCATTAAGGCCCATAAAAAAGATCATCACATATTCAGATTCCAGGTAAGAAAATAAAGATTTAGCGGCACCACAACAACATTCATCAATAGGTCTAAAATCATAGAGATCTTGCCAGAGAGTTTTAATTTTAGTAAAATAAGCTTCCATCGACAATGAACCTTGAGAAACAGTAACCAATTGGAAGTTGATAGATCTTAGGTCCATTTGATTGATGAAAACGCTCCTTCAATTCGTCCCAAACAACTTTTGTTGAACCAGTATGTATATACCAAGCTTCCCAGCAATTTCTTTAGAAATGGAATTAAGAATCCATGAGGTAACAATATCATTGTTACACTGCCAGGGGGTAAGAAGGCTCCCCGTTGGTTTTGGTATGTCAGCATTAATGAAGCCTAACTTGTTCTTACCAGAAAGAGCGCAATCAACATAGCCTTGCTCCATGAGATATAATTGTCCGTGCCGACTAGCACTTATGTGACTAAAACAGTGGTAGGTGCCGTAGTTTGATGCAACATATATGGATTAAGTTGTGCTTCAATAGCTGATGATCCATAGAAGAAGAAGAAGTCGAAGATTGTGTAGACATCGCTCAAGAGGAATAAAACGGCAAGAAATCGCGCAAAAGAAAACGCTAAGAGGAAAGCGTGTAAAAATCTTGCTCTGATACCATTATACGTATAAAATAAAATTGGTAAGGAATCAAGTTTTATTAAAATCACAATTACAATATCTCACTCATGAATAAATACTACTTGAAATACGAGTGGAAATAAAAGAAAAGATGAAAAACTGGGATAAAAGAAAATCCACAACAAACCCTAAGTTAATGCTAATTAGTTGCAACAAACTTCAATAATTTCATCAATTTCAATTTTCCAGTTTGTTTTCTGAACTGATCGTTTTCCGATAATGATCAGCTTCACATTCTCACTAGCGTGCCGACTGTTCATGAGCGTGGAAGATGAAATTCATGTAAAGAGATTATCGGGACCATTCGAGCACATTGCAGCAGGAATCATATCGATGCCGATTGATTTACCAGGAACGCCGTTCAATAGAGCAATAAAGGCGTCAAAGTTCATCAGAAAGGAAGTGGTGGCGATCGTGAGGCAGAGGAAACAGGATTTGGCGGAAGGAAAGGCGTTGGCGACGCAGGATATTTTGTCTCACATGCTTCTAACGTGCGATGAGAATGGTGTGTACATGAACGAATCAGATATCACCGATAAGATTCTTGGGTTGTTGATCGGCGGCCATGACACTGCCAGTGTCGCATGTACCTTCATCGTTAAGTTCCTCGCTGAGCTTCCTCATATCTACGATGCTGTATATACAGGTAATTAAGCTTAGTTTATGTATATATATACAAAAAGACCAAAACAGCCATAGCCTACACTACTTGCTCAAAATCAACCCTGCATTTAAATTTTTTTATTTTGTTGAGGACGTGAAATATTCCAAAGAAATGTATTTAAAATTTCAAAATTCAGGTTTAATTTGTAATTGAAAAAGGAAAAAAAAAAGTTGATTTTCCTGATATAGGGATATAGATGTAGTAATTAAAAATTAGAAAATAATGAGGTCTAACTTATTTAATCTTGTGAAATCATGTGAAATCCCAATACAAATATGATTTAAAATCATTTCAAGATTTTAAATTTATTATTTTTTCTTCGGGCAACAAACGAATTGTTCATTTTGAGATCTATTATGAATTAAATCACTCAAAATTCTGAAAGTTTAAAAGTTTACTGCCTCGAGTACTACTCTTAAAAAAATTGATTTAAGAAATAAAATTGGGAAATTTGGAATTGAACAGAGCAAATGGAAATAGCAAGAGCAAAAGCGGAAGGGGAAACGTTGAAGTGGGAAGACATTAAGAAGATGAAATATTCATGGAATGTGGCTTGTGAGGTTCTAAGACTTGCTTCCCCACTCCAAGGTGCCTTTAGGGAAGCCTTAAATGACTTCGTTTTTAATGGTTTTTTCATTCCCAAGGGTTGGAAGGTAATTTCTCCTCCCTCGATTCAAATAACATTTAGGTTTCGGTACTTTCAAGCTTTTTTTCAATTTTAATCAGATTTCGTTTTTGATTTGTCGATTCCATAACCATGATTGTAGCAAGCAAACATTATATTAGACATTGATGCTTGGTTTTTGCAGCTATATTGGAGTGCAAACTCTACACACAAAAACCCCGAGTACTTCCCAGAACCTTATAAGTTCGAGCCGGGAAGATTTGAAGGAATTGGACCATTACCCTACACATTTGTGCCGTTTGGGGGAGGGCCAAGGATGTGCCCTGGAAAGGAGTATGCAAAGCTTGAGATTTTGGTGTTCATGCATAATTTGGTGAAGAGATTCAAATGGACAAAGCTACTTGATAATGAAAACATCATTGTTAACCCAATGCCAATCCCTCAAAAAGGTCTCCCAGTTCGCCTTTTTCCTCATCAACCTCCTTCTCTTTAA

mRNA sequence

ATGGAGCTTTTCCTCATTTCTCTCTTAATCCTTTTGTTCTTCTTTCTTTCTCTTACTCTCTTCATCCTCTTCCACAATCACAAATCCTTATTCTCTTATCCCAACACTCCTCCCGGCGCCATCGGCCTTCCCATACTCGGCGAGAGCCTCGAGTTCTTATCATCTGGTTGGAGAGGCCATCCTGAGAAGTTCATCTTCGATCGTTTGAATAAGTACAAGTCAGATGTGTTCAAAACCTCGATCGTGGGCGTTCCAGCCGCCATTTTCTGCGGCCCTATTTGTAACAAGTTCCTCTTCTCTAACGAGAATAAACTGGTTACTCCTTGGTGGCCAGATTCCGTGAACAAGATCTTCCCCTCTACAACGCAGACTAGCTCCAAAGAAGAAGCTAAGAAACTCAAGAAACTCCTTCCGCAATTCCTTAAACCCGAAGCGCTTCAGCGTTATATTGGAATTATGGACGAACTTGCTGAACGCCATTTCAATTCCTTTTGGGAGAACAGAGAAGAGGTCCTCGTGTTTCCTCTTGCTAAAAGCTTCACATTCTCACTAGCGTGCCGACTGTTCATGAGCGTGGAAGATGAAATTCATGTAAAGAGATTATCGGGACCATTCGAGCACATTGCAGCAGGAATCATATCGATGCCGATTGATTTACCAGGAACGCCGTTCAATAGAGCAATAAAGGCGTCAAAGTTCATCAGAAAGGAAGTGGTGGCGATCGTGAGGCAGAGGAAACAGGATTTGGCGGAAGGAAAGGCGTTGGCGACGCAGGATATTTTGTCTCACATGCTTCTAACGTGCGATGAGAATGGTGTGTACATGAACGAATCAGATATCACCGATAAGATTCTTGGGTTGTTGATCGGCGGCCATGACACTGCCAGTGTCGCATGTACCTTCATCGTTAAGTTCCTCGCTGAGCTTCCTCATATCTACGATGCTGTATATACAGAGCAAATGGAAATAGCAAGAGCAAAAGCGGAAGGGGAAACGTTGAAGTGGGAAGACATTAAGAAGATGAAATATTCATGGAATGTGGCTTGTGAGGTTCTAAGACTTGCTTCCCCACTCCAAGGTGCCTTTAGGGAAGCCTTAAATGACTTCGTTTTTAATGGTTTTTTCATTCCCAAGGGTTGGAAGCTATATTGGAGTGCAAACTCTACACACAAAAACCCCGAGTACTTCCCAGAACCTTATAAGTTCGAGCCGGGAAGATTTGAAGGAATTGGACCATTACCCTACACATTTGTGCCGTTTGGGGGAGGGCCAAGGATGTGCCCTGGAAAGGAGTATGCAAAGCTTGAGATTTTGGTGTTCATGCATAATTTGGTGAAGAGATTCAAATGGACAAAGCTACTTGATAATGAAAACATCATTGTTAACCCAATGCCAATCCCTCAAAAAGGTCTCCCAGTTCGCCTTTTTCCTCATCAACCTCCTTCTCTTTAA

Coding sequence (CDS)

ATGGAGCTTTTCCTCATTTCTCTCTTAATCCTTTTGTTCTTCTTTCTTTCTCTTACTCTCTTCATCCTCTTCCACAATCACAAATCCTTATTCTCTTATCCCAACACTCCTCCCGGCGCCATCGGCCTTCCCATACTCGGCGAGAGCCTCGAGTTCTTATCATCTGGTTGGAGAGGCCATCCTGAGAAGTTCATCTTCGATCGTTTGAATAAGTACAAGTCAGATGTGTTCAAAACCTCGATCGTGGGCGTTCCAGCCGCCATTTTCTGCGGCCCTATTTGTAACAAGTTCCTCTTCTCTAACGAGAATAAACTGGTTACTCCTTGGTGGCCAGATTCCGTGAACAAGATCTTCCCCTCTACAACGCAGACTAGCTCCAAAGAAGAAGCTAAGAAACTCAAGAAACTCCTTCCGCAATTCCTTAAACCCGAAGCGCTTCAGCGTTATATTGGAATTATGGACGAACTTGCTGAACGCCATTTCAATTCCTTTTGGGAGAACAGAGAAGAGGTCCTCGTGTTTCCTCTTGCTAAAAGCTTCACATTCTCACTAGCGTGCCGACTGTTCATGAGCGTGGAAGATGAAATTCATGTAAAGAGATTATCGGGACCATTCGAGCACATTGCAGCAGGAATCATATCGATGCCGATTGATTTACCAGGAACGCCGTTCAATAGAGCAATAAAGGCGTCAAAGTTCATCAGAAAGGAAGTGGTGGCGATCGTGAGGCAGAGGAAACAGGATTTGGCGGAAGGAAAGGCGTTGGCGACGCAGGATATTTTGTCTCACATGCTTCTAACGTGCGATGAGAATGGTGTGTACATGAACGAATCAGATATCACCGATAAGATTCTTGGGTTGTTGATCGGCGGCCATGACACTGCCAGTGTCGCATGTACCTTCATCGTTAAGTTCCTCGCTGAGCTTCCTCATATCTACGATGCTGTATATACAGAGCAAATGGAAATAGCAAGAGCAAAAGCGGAAGGGGAAACGTTGAAGTGGGAAGACATTAAGAAGATGAAATATTCATGGAATGTGGCTTGTGAGGTTCTAAGACTTGCTTCCCCACTCCAAGGTGCCTTTAGGGAAGCCTTAAATGACTTCGTTTTTAATGGTTTTTTCATTCCCAAGGGTTGGAAGCTATATTGGAGTGCAAACTCTACACACAAAAACCCCGAGTACTTCCCAGAACCTTATAAGTTCGAGCCGGGAAGATTTGAAGGAATTGGACCATTACCCTACACATTTGTGCCGTTTGGGGGAGGGCCAAGGATGTGCCCTGGAAAGGAGTATGCAAAGCTTGAGATTTTGGTGTTCATGCATAATTTGGTGAAGAGATTCAAATGGACAAAGCTACTTGATAATGAAAACATCATTGTTAACCCAATGCCAATCCCTCAAAAAGGTCTCCCAGTTCGCCTTTTTCCTCATCAACCTCCTTCTCTTTAA

Protein sequence

MELFLISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGESLEFLSSGWRGHPEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFPSTTQTSSKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWENREEVLVFPLAKSFTFSLACRLFMSVEDEIHVKRLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVAIVRQRKQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACTFIVKFLAELPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLRLASPLQGAFREALNDFVFNGFFIPKGWKLYWSANSTHKNPEYFPEPYKFEPGRFEGIGPLPYTFVPFGGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLDNENIIVNPMPIPQKGLPVRLFPHQPPSL*
Homology
BLAST of Chy2G038770 vs. ExPASy Swiss-Prot
Match: F6H9N6 (Beta-amyrin 28-monooxygenase OS=Vitis vinifera OX=29760 GN=CYP716A15 PE=1 SV=1)

HSP 1 Score: 731.5 bits (1887), Expect = 6.3e-210
Identity = 344/480 (71.67%), Postives = 409/480 (85.21%), Query Frame = 0

Query: 1   MELFLISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGESLEFLSSGWRGH 60
           ME+F +SLL++    +S+ L +LF+ H+S F+ PN PPG IG P++GESLEFLS+GW+GH
Sbjct: 1   MEVFFLSLLLIFVLSVSIGLHLLFYKHRSHFTGPNLPPGKIGWPMVGESLEFLSTGWKGH 60

Query: 61  PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFPS 120
           PEKFIFDR++KY S+VFKTS++G PAA+F G   NKFLFSNENKLV  WWP SV+K+FPS
Sbjct: 61  PEKFIFDRISKYSSEVFKTSLLGEPAAVFAGAAGNKFLFSNENKLVHAWWPSSVDKVFPS 120

Query: 121 TTQTSSKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWENREEVLVFPLAKSF 180
           +TQTSSKEEAKK++KLLPQF KPEALQRYIGIMD +A+RHF   W+NR+EV+VFPLAK F
Sbjct: 121 STQTSSKEEAKKMRKLLPQFFKPEALQRYIGIMDHIAQRHFADSWDNRDEVIVFPLAKRF 180

Query: 181 TFSLACRLFMSVEDEIHVKRLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVA 240
           TF LACRLFMS+ED  HV +   PF  +A+G+I++PIDLPGTPF+RAIKAS FIRKE+ A
Sbjct: 181 TFWLACRLFMSIEDPAHVAKFEKPFHVLASGLITVPIDLPGTPFHRAIKASNFIRKELRA 240

Query: 241 IVRQRKQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACT 300
           I++QRK DLAEGKA   QDILSHMLL  DE+G +MNE +I DKILGLLIGGHDTAS A T
Sbjct: 241 IIKQRKIDLAEGKASQNQDILSHMLLATDEDGCHMNEMEIADKILGLLIGGHDTASAAIT 300

Query: 301 FIVKFLAELPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLRLASPLQG 360
           F++K++AELPHIY+ VY EQMEIA +KA GE L W+D++KM+YSWNVACEV+RLA PLQG
Sbjct: 301 FLIKYMAELPHIYEKVYEEQMEIANSKAPGELLNWDDVQKMRYSWNVACEVMRLAPPLQG 360

Query: 361 AFREALNDFVFNGFFIPKGWKLYWSANSTHKNPEYFPEPYKFEPGRFEGIGPLPYTFVPF 420
           AFREA+ DFVFNGF IPKGWKLYWSANSTHK+PE FP+P  F+P RFEG GP PYTFVPF
Sbjct: 361 AFREAITDFVFNGFSIPKGWKLYWSANSTHKSPECFPQPENFDPTRFEGDGPAPYTFVPF 420

Query: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLDNENIIVNPMPIPQKGLPVRLFPHQP 480
           GGGPRMCPGKEYA+LEILVFMHN+VKRFKW KLL +E IIV+PMP+P KGLPVRL P +P
Sbjct: 421 GGGPRMCPGKEYARLEILVFMHNVVKRFKWDKLLPDEKIIVDPMPMPAKGLPVRLHPRKP 480

BLAST of Chy2G038770 vs. ExPASy Swiss-Prot
Match: A5BFI4 (Beta-amyrin 28-monooxygenase OS=Vitis vinifera OX=29760 GN=CYP716A17 PE=1 SV=2)

HSP 1 Score: 731.5 bits (1887), Expect = 6.3e-210
Identity = 345/480 (71.88%), Postives = 409/480 (85.21%), Query Frame = 0

Query: 1   MELFLISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGESLEFLSSGWRGH 60
           ME+F +SLL++    +S+ L++L + H+S F+ PN PPG IG P++GESLEFLS+GW+GH
Sbjct: 1   MEVFFLSLLLICVLSVSIRLYLLLYKHRSHFTGPNLPPGKIGWPMVGESLEFLSTGWKGH 60

Query: 61  PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFPS 120
           PEKFIFDR++KY S+VFKTS++G PAA+F G   NKFLFSNENKLV  WWP SV+K+FPS
Sbjct: 61  PEKFIFDRISKYSSEVFKTSLLGEPAAVFAGAAGNKFLFSNENKLVHAWWPSSVDKVFPS 120

Query: 121 TTQTSSKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWENREEVLVFPLAKSF 180
           +TQTSSKEEAKK++KLLPQFLKPEALQRY GIMD +A+RHF   W+NR+EV+VFPLAK F
Sbjct: 121 STQTSSKEEAKKMRKLLPQFLKPEALQRYTGIMDHIAQRHFADSWDNRDEVIVFPLAKRF 180

Query: 181 TFSLACRLFMSVEDEIHVKRLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVA 240
           TF LACRLFMS+ED  HV +   PF  +A+G+I++PIDLPGTPF+RAIKAS FIRKE+ A
Sbjct: 181 TFWLACRLFMSIEDPAHVAKFEKPFHVLASGLITIPIDLPGTPFHRAIKASNFIRKELRA 240

Query: 241 IVRQRKQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACT 300
           I++QRK DLAE KA  TQDILSHMLL  DE+G +MNE  I DKILGLLIGGHDTAS A T
Sbjct: 241 IIKQRKIDLAESKASKTQDILSHMLLATDEDGCHMNEMSIADKILGLLIGGHDTASSAIT 300

Query: 301 FIVKFLAELPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLRLASPLQG 360
           F+VK++AELPHIY+ VY EQMEIA +KA GE L W+D++KM+YSWNVACEV+RLA PLQG
Sbjct: 301 FLVKYMAELPHIYEKVYKEQMEIANSKAPGELLNWDDVQKMRYSWNVACEVMRLAPPLQG 360

Query: 361 AFREALNDFVFNGFFIPKGWKLYWSANSTHKNPEYFPEPYKFEPGRFEGIGPLPYTFVPF 420
           AFREA+ DFVFNGF IPKGWKLYWSANSTHK+ E FP+P KF+P RFEG GP PYTFVPF
Sbjct: 361 AFREAITDFVFNGFSIPKGWKLYWSANSTHKSLECFPQPEKFDPTRFEGAGPAPYTFVPF 420

Query: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLDNENIIVNPMPIPQKGLPVRLFPHQP 480
           GGGPRMCPGKEYA+LEIL+FMHNLVKRFKW KLL +E IIV+PMP+P KGLPVRL PH+P
Sbjct: 421 GGGPRMCPGKEYARLEILIFMHNLVKRFKWDKLLPDEKIIVDPMPMPAKGLPVRLHPHKP 480

BLAST of Chy2G038770 vs. ExPASy Swiss-Prot
Match: A0A0S2II38 (Beta-amyrin 28-monooxygenase OS=Kalopanax septemlobus OX=228393 GN=CYP716A94 PE=1 SV=1)

HSP 1 Score: 718.4 bits (1853), Expect = 5.5e-206
Identity = 342/480 (71.25%), Postives = 401/480 (83.54%), Query Frame = 0

Query: 1   MELFLISLLILLFFFLSLTLFILFHNHKS-LFSYPNTPPGAIGLPILGESLEFLSSGWRG 60
           M+LF + LL L   F+SL+ + LF+  KS   S    PPG  G P++GES EFLS+GW+G
Sbjct: 1   MQLFYVPLLSLFVLFVSLSFYFLFYKSKSGSTSGLPLPPGKTGWPVIGESFEFLSTGWKG 60

Query: 61  HPEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFP 120
           +PEKFIFDRL KY S+VFKTS+ G PAA FCG  CNKFLFSNENKLV  WWPDSVNK+FP
Sbjct: 61  YPEKFIFDRLAKYSSNVFKTSLFGQPAAAFCGAACNKFLFSNENKLVQAWWPDSVNKVFP 120

Query: 121 STTQTSSKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWENREEVLVFPLAKS 180
           S+TQTSSKEEA K++KLLP FLKPEALQRY+G+MD++A +HF S WEN++EVLVFPLAK+
Sbjct: 121 SSTQTSSKEEAIKMRKLLPNFLKPEALQRYVGVMDQIATKHFKSGWENKKEVLVFPLAKN 180

Query: 181 FTFSLACRLFMSVEDEIHVKRLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVV 240
           +TF +AC++F+SVE+   V +L  PF  IA+GIIS+PIDLPGTPFN AIK+SK +R +++
Sbjct: 181 YTFWIACKVFVSVEEPTQVAKLLEPFNAIASGIISVPIDLPGTPFNSAIKSSKIVRDKLM 240

Query: 241 AIVRQRKQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVAC 300
            I++QRK DL EGKA  TQDILSHMLLT DENG +M E DI DKILGLLIGGHDTAS AC
Sbjct: 241 GIIKQRKTDLGEGKASPTQDILSHMLLTSDENGKFMTEGDIADKILGLLIGGHDTASSAC 300

Query: 301 TFIVKFLAELPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLRLASPLQ 360
           TF+VKFLAELP IY+ VY EQMEIA++K  GE L WEDI+KMKYSWNVACEVLRLA PLQ
Sbjct: 301 TFVVKFLAELPEIYEGVYKEQMEIAKSKKAGELLNWEDIQKMKYSWNVACEVLRLAPPLQ 360

Query: 361 GAFREALNDFVFNGFFIPKGWKLYWSANSTHKNPEYFPEPYKFEPGRFEGIGPLPYTFVP 420
           GAFREAL DF +NGF IPKGWKLYWSANSTH+N E FPEP KF+P RFEG GP PY+FVP
Sbjct: 361 GAFREALTDFSYNGFSIPKGWKLYWSANSTHRNSEVFPEPLKFDPSRFEGAGPPPYSFVP 420

Query: 421 FGGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLDNENIIVNPMPIPQKGLPVRLFPHQ 480
           FGGGPRMCPGKEYA+LEILVFMH++VKRFKW K++ +E I+VNPMPIP KGLPVRLFPH+
Sbjct: 421 FGGGPRMCPGKEYARLEILVFMHHVVKRFKWEKVIPDEKIVVNPMPIPAKGLPVRLFPHK 480

BLAST of Chy2G038770 vs. ExPASy Swiss-Prot
Match: Q2MJ20 (Beta-amyrin 28-monooxygenase OS=Medicago truncatula OX=3880 GN=CYP716A12 PE=1 SV=1)

HSP 1 Score: 714.5 bits (1843), Expect = 7.9e-205
Identity = 340/477 (71.28%), Postives = 399/477 (83.65%), Query Frame = 0

Query: 4   FLISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGESLEFLSSGWRGHPEK 63
           F +SLL+L   F+SL+LF +F+  KS     N PPG +G PI+GESLEFLS+GW+GHPEK
Sbjct: 5   FYLSLLLLFVTFISLSLFFIFYKQKSPL---NLPPGKMGYPIIGESLEFLSTGWKGHPEK 64

Query: 64  FIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFPSTTQ 123
           FIFDR+ KY S++FKTSIVG    + CG   NKFLFSNENKLVT WWPDSVNKIFP+T+ 
Sbjct: 65  FIFDRMRKYSSELFKTSIVGESTVVCCGAASNKFLFSNENKLVTAWWPDSVNKIFPTTSL 124

Query: 124 TSS-KEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWENREEVLVFPLAKSFTF 183
            S+ KEE+ K++KLLPQF KPEALQRY+G+MD +A+RHF + W+N+ E+ V+PLAK +TF
Sbjct: 125 DSNLKEESIKMRKLLPQFFKPEALQRYVGVMDVIAQRHFVTHWDNKNEITVYPLAKRYTF 184

Query: 184 SLACRLFMSVEDEIHVKRLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVAIV 243
            LACRLFMSVEDE HV + S PF+ IAAGIIS+PIDLPGTPFN+AIKAS FIRKE++ I+
Sbjct: 185 LLACRLFMSVEDENHVAKFSDPFQLIAAGIISLPIDLPGTPFNKAIKASNFIRKELIKII 244

Query: 244 RQRKQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACTFI 303
           +QR+ DLAEG A  TQDILSHMLLT DENG  MNE +I DKILGLLIGGHDTASVACTF+
Sbjct: 245 KQRRVDLAEGTASPTQDILSHMLLTSDENGKSMNELNIADKILGLLIGGHDTASVACTFL 304

Query: 304 VKFLAELPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLRLASPLQGAF 363
           VK+L ELPHIYD VY EQMEIA++K  GE L W+D+KKMKYSWNVACEV+RL+ PLQG F
Sbjct: 305 VKYLGELPHIYDKVYQEQMEIAKSKPAGELLNWDDLKKMKYSWNVACEVMRLSPPLQGGF 364

Query: 364 REALNDFVFNGFFIPKGWKLYWSANSTHKNPEYFPEPYKFEPGRFEGIGPLPYTFVPFGG 423
           REA+ DF+FNGF IPKGWKLYWSANSTHKN E FP P KF+P RFEG GP PYTFVPFGG
Sbjct: 365 REAITDFMFNGFSIPKGWKLYWSANSTHKNAECFPMPEKFDPTRFEGNGPAPYTFVPFGG 424

Query: 424 GPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLDNENIIVNPMPIPQKGLPVRLFPHQ 480
           GPRMCPGKEYA+LEILVFMHNLVKRFKW K++ +E IIV+P PIP K LP+RL+PH+
Sbjct: 425 GPRMCPGKEYARLEILVFMHNLVKRFKWEKVIPDEKIIVDPFPIPAKDLPIRLYPHK 478

BLAST of Chy2G038770 vs. ExPASy Swiss-Prot
Match: I7C6E8 (Beta-amyrin 28-monooxygenase OS=Panax ginseng OX=4054 GN=CYP716A52v2 PE=1 SV=1)

HSP 1 Score: 713.8 bits (1841), Expect = 1.4e-204
Identity = 342/481 (71.10%), Postives = 399/481 (82.95%), Query Frame = 0

Query: 1   MELFLISLLILLFFFLSLTLFILFHNHK--SLFSYPNTPPGAIGLPILGESLEFLSSGWR 60
           MELF + LL L   F+SL+   LF+  K  S   +P  PPG  G PI+GES EFLS+GW+
Sbjct: 1   MELFYVPLLSLFVLFISLSFHFLFYKSKPSSSGGFP-LPPGKTGWPIIGESYEFLSTGWK 60

Query: 61  GHPEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIF 120
           G+PEKFIFDR+ KY S+VFKTSI G PAA+FCG  CNKFLFSNENKLV  WWPDSVNK+F
Sbjct: 61  GYPEKFIFDRMTKYSSNVFKTSIFGEPAAVFCGAACNKFLFSNENKLVQAWWPDSVNKVF 120

Query: 121 PSTTQTSSKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWENREEVLVFPLAK 180
           PS+TQTSSKEEA K++K+LP F KPEALQRYIG+MD++A  HF S WEN+ EV+VFPLAK
Sbjct: 121 PSSTQTSSKEEAIKMRKMLPNFFKPEALQRYIGLMDQIAANHFESGWENKNEVVVFPLAK 180

Query: 181 SFTFSLACRLFMSVEDEIHVKRLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEV 240
           S+TF +AC++F+SVE+   V  L  PF  IA+GIIS+PIDLPGTPFN AIK+SK +R+++
Sbjct: 181 SYTFWIACKVFVSVEEPAQVAELLEPFSAIASGIISVPIDLPGTPFNSAIKSSKIVRRKL 240

Query: 241 VAIVRQRKQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVA 300
           V I++QRK DL EGKA ATQDILSHMLLT DE+G +M E DI DKILGLLIGGHDTAS A
Sbjct: 241 VGIIKQRKIDLGEGKASATQDILSHMLLTSDESGKFMGEGDIADKILGLLIGGHDTASSA 300

Query: 301 CTFIVKFLAELPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLRLASPL 360
           CTF+VKFLAELP IY+ VY EQMEI ++K  GE LKWEDI+KMKYSWNVACEVLRLA PL
Sbjct: 301 CTFVVKFLAELPQIYEGVYQEQMEIVKSKKAGELLKWEDIQKMKYSWNVACEVLRLAPPL 360

Query: 361 QGAFREALNDFVFNGFFIPKGWKLYWSANSTHKNPEYFPEPYKFEPGRFEGIGPLPYTFV 420
           QGAFREAL+DF +NGF IPKGWKLYWSANSTH N E FPEP KF+P RF+G GP P++FV
Sbjct: 361 QGAFREALSDFTYNGFSIPKGWKLYWSANSTHINSEVFPEPLKFDPSRFDGAGPPPFSFV 420

Query: 421 PFGGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLDNENIIVNPMPIPQKGLPVRLFPH 480
           PFGGGPRMCPGKEYA+LEILVFMH+LVKRFKW K++ +E I+VNPMPIP  GLPVRLFPH
Sbjct: 421 PFGGGPRMCPGKEYARLEILVFMHHLVKRFKWEKVIPDEKIVVNPMPIPANGLPVRLFPH 480

BLAST of Chy2G038770 vs. ExPASy TrEMBL
Match: A0A1S3B8H4 (beta-amyrin 28-oxidase OS=Cucumis melo OX=3656 GN=LOC103487187 PE=3 SV=1)

HSP 1 Score: 972.6 bits (2513), Expect = 6.0e-280
Identity = 477/483 (98.76%), Postives = 480/483 (99.38%), Query Frame = 0

Query: 1   MELFLISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGESLEFLSSGWRGH 60
           ME FLISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGESLEFLSSGWRGH
Sbjct: 1   MEHFLISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGESLEFLSSGWRGH 60

Query: 61  PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFPS 120
           PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFPS
Sbjct: 61  PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFPS 120

Query: 121 TTQTSSKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWENREEVLVFPLAKSF 180
           TTQTSSKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFW+NREEVLVFPLAKSF
Sbjct: 121 TTQTSSKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWKNREEVLVFPLAKSF 180

Query: 181 TFSLACRLFMSVEDEIHVKRLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVA 240
           TFSLACRLFMSVEDEIHV+RLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVA
Sbjct: 181 TFSLACRLFMSVEDEIHVERLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVA 240

Query: 241 IVRQRKQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACT 300
           IVRQRKQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACT
Sbjct: 241 IVRQRKQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACT 300

Query: 301 FIVKFLAELPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLRLASPLQG 360
           FIVKFLAELPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLRLASPLQG
Sbjct: 301 FIVKFLAELPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLRLASPLQG 360

Query: 361 AFREALNDFVFNGFFIPKGWKLYWSANSTHKNPEYFPEPYKFEPGRFEGIGPLPYTFVPF 420
           AFREALNDFVFNGFFIPKGWKLYWSANSTHKNPEYFPEPYKFEPGRFEG GPLPYTFVPF
Sbjct: 361 AFREALNDFVFNGFFIPKGWKLYWSANSTHKNPEYFPEPYKFEPGRFEGNGPLPYTFVPF 420

Query: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLDNENIIVNPMPIPQKGLPVRLFPHQP 480
           GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLL+NENIIVNPMPIPQKGLPVRLFPHQP
Sbjct: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLENENIIVNPMPIPQKGLPVRLFPHQP 480

Query: 481 PSL 484
            SL
Sbjct: 481 LSL 483

BLAST of Chy2G038770 vs. ExPASy TrEMBL
Match: A0A0A0LXN3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G649550 PE=3 SV=1)

HSP 1 Score: 967.2 bits (2499), Expect = 2.5e-278
Identity = 471/483 (97.52%), Postives = 481/483 (99.59%), Query Frame = 0

Query: 1   MELFLISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGESLEFLSSGWRGH 60
           MELFLISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGES+EFLSSGW+GH
Sbjct: 1   MELFLISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGESVEFLSSGWKGH 60

Query: 61  PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFPS 120
           PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFPS
Sbjct: 61  PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFPS 120

Query: 121 TTQTSSKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWENREEVLVFPLAKSF 180
           TTQTS+KEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFW+NREEVLVFPLAKSF
Sbjct: 121 TTQTSTKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWKNREEVLVFPLAKSF 180

Query: 181 TFSLACRLFMSVEDEIHVKRLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVA 240
           TFS+ACRLFMSVEDEIHV+RLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVA
Sbjct: 181 TFSIACRLFMSVEDEIHVERLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVA 240

Query: 241 IVRQRKQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACT 300
           IVRQRKQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACT
Sbjct: 241 IVRQRKQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACT 300

Query: 301 FIVKFLAELPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLRLASPLQG 360
           FIVKFLAELPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLR+ASPLQG
Sbjct: 301 FIVKFLAELPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLRIASPLQG 360

Query: 361 AFREALNDFVFNGFFIPKGWKLYWSANSTHKNPEYFPEPYKFEPGRFEGIGPLPYTFVPF 420
           AFREAL+DFVFNGFFIPKGWKLYWSANSTHKNPEYFPEPYKF+PGRFEG GPLPYTFVPF
Sbjct: 361 AFREALSDFVFNGFFIPKGWKLYWSANSTHKNPEYFPEPYKFDPGRFEGNGPLPYTFVPF 420

Query: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLDNENIIVNPMPIPQKGLPVRLFPHQP 480
           GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLL+NENIIVNPMPIPQKGLPVRLFPHQP
Sbjct: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLENENIIVNPMPIPQKGLPVRLFPHQP 480

Query: 481 PSL 484
            SL
Sbjct: 481 LSL 483

BLAST of Chy2G038770 vs. ExPASy TrEMBL
Match: A0A6J1JJQ7 (beta-amyrin 28-oxidase OS=Cucurbita maxima OX=3661 GN=LOC111485071 PE=3 SV=1)

HSP 1 Score: 839.0 bits (2166), Expect = 1.0e-239
Identity = 407/485 (83.92%), Postives = 444/485 (91.55%), Query Frame = 0

Query: 1   MELFLISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGESLEFLSSGWRGH 60
           ME FLISLLIL   F+SLTLF+LF+ HKSLFSYPNTPPGAIGLPI+GESLEFLS+GW+G 
Sbjct: 1   MEDFLISLLILFLSFISLTLFVLFYKHKSLFSYPNTPPGAIGLPIIGESLEFLSTGWKGE 60

Query: 61  PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFPS 120
           PEKFIFDRLNKYKSD+FKTSIVGVPAAIFCGP+CNKFLFSNENKLVT WWPDSVNKIFP+
Sbjct: 61  PEKFIFDRLNKYKSDIFKTSIVGVPAAIFCGPVCNKFLFSNENKLVTSWWPDSVNKIFPA 120

Query: 121 TTQTSSKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWENREEVLVFPLAKSF 180
           +T+ +SKE A +LKKLLP+FLKPEALQRYIG+MDELA+RHF S WENRE VLVFPLAKSF
Sbjct: 121 STEINSKESAIRLKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENREHVLVFPLAKSF 180

Query: 181 TFSLACRLFMSVEDEIHVKRLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVA 240
           TFSLACRLF+S+ED IHV+RLS PF++IAAGIISMPIDLPGTPFNRAIKAS F+RKE+++
Sbjct: 181 TFSLACRLFLSLEDRIHVERLSEPFQNIAAGIISMPIDLPGTPFNRAIKASNFVRKELLS 240

Query: 241 IVRQRKQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACT 300
           IVRQRK DLAEGKA  TQDILSHMLLTCDE G YMNES I D ILGLLIGGHDTAS ACT
Sbjct: 241 IVRQRKSDLAEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHDTASAACT 300

Query: 301 FIVKFLAELPHIYDAVYTEQMEI--ARAKAEGETLKWEDIKKMKYSWNVACEVLRLASPL 360
           FIVK+LAELP IYD VY EQMEI  A+AKA+GE LKWEDI+KMKYSWNVACEVLRLASPL
Sbjct: 301 FIVKYLAELPQIYDRVYREQMEIAKAKAKAKGELLKWEDIQKMKYSWNVACEVLRLASPL 360

Query: 361 QGAFREALNDFVFNGFFIPKGWKLYWSANSTHKNPEYFPEPYKFEPGRFEGIGPLPYTFV 420
           QGAFREA+NDFVFNGF IPKGWKLYWS+NSTHKN  YFPEPYKF+P RFEG GP PYTFV
Sbjct: 361 QGAFREAINDFVFNGFNIPKGWKLYWSSNSTHKNHAYFPEPYKFDPSRFEGNGPAPYTFV 420

Query: 421 PFGGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLDNENIIVNPMPIPQKGLPVRLFPH 480
           PFGGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLL+ E IIV+P+PIP+KGLPVRLFPH
Sbjct: 421 PFGGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLEKEKIIVDPLPIPEKGLPVRLFPH 480

Query: 481 QPPSL 484
           +  SL
Sbjct: 481 KTLSL 485

BLAST of Chy2G038770 vs. ExPASy TrEMBL
Match: A0A6J1H8T4 (beta-amyrin 28-oxidase OS=Cucurbita moschata OX=3662 GN=LOC111461545 PE=3 SV=1)

HSP 1 Score: 833.6 bits (2152), Expect = 4.3e-238
Identity = 401/480 (83.54%), Postives = 436/480 (90.83%), Query Frame = 0

Query: 4   FLISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGESLEFLSSGWRGHPEK 63
           FLISLLIL    +SLTLF+LF+ HKSLFSYPNTPPGAIGLPI+GESLEFLS+GW+G PEK
Sbjct: 4   FLISLLILFLSLISLTLFVLFYKHKSLFSYPNTPPGAIGLPIIGESLEFLSTGWKGEPEK 63

Query: 64  FIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFPSTTQ 123
           FIFDRLNKYKSD+FKTSIVGVPAAIFCGP CNKFLFSNENKLVT WWPDSVNKIFP++T+
Sbjct: 64  FIFDRLNKYKSDIFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPASTE 123

Query: 124 TSSKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWENREEVLVFPLAKSFTFS 183
            +SKE A +LKKLLP+FLKPEALQRYIG+MDELA+RHF S WENRE+VLVFPLAKSFTFS
Sbjct: 124 INSKESAIRLKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENREQVLVFPLAKSFTFS 183

Query: 184 LACRLFMSVEDEIHVKRLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVAIVR 243
           LACRLF+S+ED IHV+RLS PF+ IAAGIISMPIDLPGTPFNRAIKAS F+RKE+++IVR
Sbjct: 184 LACRLFVSLEDRIHVERLSEPFQSIAAGIISMPIDLPGTPFNRAIKASNFVRKELLSIVR 243

Query: 244 QRKQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACTFIV 303
           QRK DLAEGKA  TQDILSHMLLTCDE G YMNES I D ILGLLIGGHDT S ACTFIV
Sbjct: 244 QRKSDLAEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHDTTSAACTFIV 303

Query: 304 KFLAELPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLRLASPLQGAFR 363
            +LAELP IYD VY EQMEIA+AKA GE LKWEDI+KMKYSWNVACEVLRLASPLQGAFR
Sbjct: 304 NYLAELPQIYDGVYREQMEIAKAKAAGELLKWEDIQKMKYSWNVACEVLRLASPLQGAFR 363

Query: 364 EALNDFVFNGFFIPKGWKLYWSANSTHKNPEYFPEPYKFEPGRFEGIGPLPYTFVPFGGG 423
           EA+N FVFNGF+IPKGWK+YWS+NSTHKN  YFPEPYKF+P RFEG GP PYTFVPFGGG
Sbjct: 364 EAINHFVFNGFYIPKGWKVYWSSNSTHKNHAYFPEPYKFDPSRFEGNGPAPYTFVPFGGG 423

Query: 424 PRMCPGKEYAKLEILVFMHNLVKRFKWTKLLDNENIIVNPMPIPQKGLPVRLFPHQPPSL 483
           PRMCPGKEYAKLEILVFMHNLVKRFKWTKLLD E IIV+P+PIP+KGLPVRLFPH   SL
Sbjct: 424 PRMCPGKEYAKLEILVFMHNLVKRFKWTKLLDKEKIIVDPLPIPEKGLPVRLFPHTTLSL 483

BLAST of Chy2G038770 vs. ExPASy TrEMBL
Match: A0A6J1DZX7 (beta-amyrin 28-oxidase OS=Momordica charantia OX=3673 GN=LOC111024635 PE=3 SV=1)

HSP 1 Score: 827.4 bits (2136), Expect = 3.1e-236
Identity = 395/482 (81.95%), Postives = 430/482 (89.21%), Query Frame = 0

Query: 1   MELFLISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGESLEFLSSGWRGH 60
           ME FL+SLLI     + + LFILF+ HKS FSYPN PPG IG PI+GESLEF S+GW+G 
Sbjct: 1   MEHFLVSLLIFFVSVVCVGLFILFYKHKSPFSYPNIPPGGIGFPIIGESLEFFSTGWKGE 60

Query: 61  PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFPS 120
           PEKFIFDR+NKYKS +FKTSIVG PAA+FCGP CNKFLFSNENKLVT WWPDSVNKIFPS
Sbjct: 61  PEKFIFDRINKYKSHIFKTSIVGAPAAVFCGPTCNKFLFSNENKLVTAWWPDSVNKIFPS 120

Query: 121 TTQTSSKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWENREEVLVFPLAKSF 180
           +TQTSS EEAKKLKKLLPQFLKPEAL RYIGIMD LA RHF S WEN+++VLVFPLAKSF
Sbjct: 121 STQTSSMEEAKKLKKLLPQFLKPEALHRYIGIMDALAHRHFASSWENKDQVLVFPLAKSF 180

Query: 181 TFSLACRLFMSVEDEIHVKRLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVA 240
           TFSLACRLF+SVEDE HV+RLSGPF+HIAAGIISMPID PGTPFNR IKAS FIRKE+++
Sbjct: 181 TFSLACRLFVSVEDEAHVERLSGPFQHIAAGIISMPIDFPGTPFNRGIKASNFIRKELLS 240

Query: 241 IVRQRKQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACT 300
           IV+QRK DLAEGKA  TQDILSHMLLTCDENG YMNE DI DKILGLLIGGHDTAS ACT
Sbjct: 241 IVKQRKVDLAEGKASPTQDILSHMLLTCDENGAYMNELDIADKILGLLIGGHDTASAACT 300

Query: 301 FIVKFLAELPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLRLASPLQG 360
           FIVKFLAELPHIYD VY EQM IA+AKA GE L WEDI+KMKYSWNVACEVLRLASPLQG
Sbjct: 301 FIVKFLAELPHIYDGVYKEQMAIAKAKAPGELLNWEDIQKMKYSWNVACEVLRLASPLQG 360

Query: 361 AFREALNDFVFNGFFIPKGWKLYWSANSTHKNPEYFPEPYKFEPGRFEGIGPLPYTFVPF 420
           AFREA+NDFVFNGF+IPKGWKLYWS+NSTHKN +YFP+PY F+P RFEG GP PYTFVPF
Sbjct: 361 AFREAINDFVFNGFYIPKGWKLYWSSNSTHKNADYFPDPYTFDPSRFEGNGPAPYTFVPF 420

Query: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLDNENIIVNPMPIPQKGLPVRLFPHQP 480
           GGGPRMCPGKEYA+LEILVFMHNLVKRFKW K+L+ E I+V+PMPIPQKGLPVRLFPH+ 
Sbjct: 421 GGGPRMCPGKEYARLEILVFMHNLVKRFKWAKILEKEKIVVDPMPIPQKGLPVRLFPHKT 480

Query: 481 PS 483
            S
Sbjct: 481 VS 482

BLAST of Chy2G038770 vs. NCBI nr
Match: XP_008443638.1 (PREDICTED: beta-amyrin 28-oxidase [Cucumis melo])

HSP 1 Score: 967 bits (2501), Expect = 0.0
Identity = 477/483 (98.76%), Postives = 480/483 (99.38%), Query Frame = 0

Query: 1   MELFLISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGESLEFLSSGWRGH 60
           ME FLISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGESLEFLSSGWRGH
Sbjct: 1   MEHFLISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGESLEFLSSGWRGH 60

Query: 61  PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFPS 120
           PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFPS
Sbjct: 61  PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFPS 120

Query: 121 TTQTSSKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWENREEVLVFPLAKSF 180
           TTQTSSKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFW+NREEVLVFPLAKSF
Sbjct: 121 TTQTSSKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWKNREEVLVFPLAKSF 180

Query: 181 TFSLACRLFMSVEDEIHVKRLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVA 240
           TFSLACRLFMSVEDEIHV+RLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVA
Sbjct: 181 TFSLACRLFMSVEDEIHVERLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVA 240

Query: 241 IVRQRKQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACT 300
           IVRQRKQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACT
Sbjct: 241 IVRQRKQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACT 300

Query: 301 FIVKFLAELPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLRLASPLQG 360
           FIVKFLAELPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLRLASPLQG
Sbjct: 301 FIVKFLAELPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLRLASPLQG 360

Query: 361 AFREALNDFVFNGFFIPKGWKLYWSANSTHKNPEYFPEPYKFEPGRFEGIGPLPYTFVPF 420
           AFREALNDFVFNGFFIPKGWKLYWSANSTHKNPEYFPEPYKFEPGRFEG GPLPYTFVPF
Sbjct: 361 AFREALNDFVFNGFFIPKGWKLYWSANSTHKNPEYFPEPYKFEPGRFEGNGPLPYTFVPF 420

Query: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLDNENIIVNPMPIPQKGLPVRLFPHQP 480
           GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLL+NENIIVNPMPIPQKGLPVRLFPHQP
Sbjct: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLENENIIVNPMPIPQKGLPVRLFPHQP 480

Query: 481 PSL 483
            SL
Sbjct: 481 LSL 483

BLAST of Chy2G038770 vs. NCBI nr
Match: XP_004139087.1 (beta-amyrin 28-monooxygenase [Cucumis sativus] >KGN66615.1 hypothetical protein Csa_007564 [Cucumis sativus])

HSP 1 Score: 962 bits (2487), Expect = 0.0
Identity = 471/483 (97.52%), Postives = 481/483 (99.59%), Query Frame = 0

Query: 1   MELFLISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGESLEFLSSGWRGH 60
           MELFLISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGES+EFLSSGW+GH
Sbjct: 1   MELFLISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGESVEFLSSGWKGH 60

Query: 61  PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFPS 120
           PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFPS
Sbjct: 61  PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFPS 120

Query: 121 TTQTSSKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWENREEVLVFPLAKSF 180
           TTQTS+KEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFW+NREEVLVFPLAKSF
Sbjct: 121 TTQTSTKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWKNREEVLVFPLAKSF 180

Query: 181 TFSLACRLFMSVEDEIHVKRLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVA 240
           TFS+ACRLFMSVEDEIHV+RLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVA
Sbjct: 181 TFSIACRLFMSVEDEIHVERLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVA 240

Query: 241 IVRQRKQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACT 300
           IVRQRKQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACT
Sbjct: 241 IVRQRKQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACT 300

Query: 301 FIVKFLAELPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLRLASPLQG 360
           FIVKFLAELPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLR+ASPLQG
Sbjct: 301 FIVKFLAELPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLRIASPLQG 360

Query: 361 AFREALNDFVFNGFFIPKGWKLYWSANSTHKNPEYFPEPYKFEPGRFEGIGPLPYTFVPF 420
           AFREAL+DFVFNGFFIPKGWKLYWSANSTHKNPEYFPEPYKF+PGRFEG GPLPYTFVPF
Sbjct: 361 AFREALSDFVFNGFFIPKGWKLYWSANSTHKNPEYFPEPYKFDPGRFEGNGPLPYTFVPF 420

Query: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLDNENIIVNPMPIPQKGLPVRLFPHQP 480
           GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLL+NENIIVNPMPIPQKGLPVRLFPHQP
Sbjct: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLENENIIVNPMPIPQKGLPVRLFPHQP 480

Query: 481 PSL 483
            SL
Sbjct: 481 LSL 483

BLAST of Chy2G038770 vs. NCBI nr
Match: XP_038877759.1 (LOW QUALITY PROTEIN: beta-amyrin 28-monooxygenase-like [Benincasa hispida])

HSP 1 Score: 898 bits (2320), Expect = 0.0
Identity = 438/483 (90.68%), Postives = 457/483 (94.62%), Query Frame = 0

Query: 1   MELFLISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGESLEFLSSGWRGH 60
           ME FLISLLI   FFLSLTLFILFHNHKSLFSYPN PPGAIGLPILGESLEFLS+GW+G 
Sbjct: 1   MEHFLISLLIFFLFFLSLTLFILFHNHKSLFSYPNVPPGAIGLPILGESLEFLSTGWKGE 60

Query: 61  PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFPS 120
           PEKFIFDRL+KYKSDVFKTSIVGVPAAIFCG  CNKFLFSNENKLVTPWWPDSVNKIFPS
Sbjct: 61  PEKFIFDRLHKYKSDVFKTSIVGVPAAIFCGSACNKFLFSNENKLVTPWWPDSVNKIFPS 120

Query: 121 TTQTSSKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWENREEVLVFPLAKSF 180
           +TQTSSKEEAKKLKK LPQFLKPEALQRYIG+MDELA+RHF SFWENREEVLVFPLAKSF
Sbjct: 121 STQTSSKEEAKKLKKFLPQFLKPEALQRYIGVMDELAQRHFASFWENREEVLVFPLAKSF 180

Query: 181 TFSLACRLFMSVEDEIHVKRLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVA 240
           TFSLACRLF+SVEDEIHVKRLSGPFEHIAAGIIS+PIDLPGTPFNRAIKASKFIRKE+VA
Sbjct: 181 TFSLACRLFVSVEDEIHVKRLSGPFEHIAAGIISVPIDLPGTPFNRAIKASKFIRKELVA 240

Query: 241 IVRQRKQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACT 300
           IV++R+ DLAEGKA ATQDILSHMLLTCDENGV+MNESDI DKILGLLIGGHDTASVACT
Sbjct: 241 IVKKRRWDLAEGKASATQDILSHMLLTCDENGVFMNESDIADKILGLLIGGHDTASVACT 300

Query: 301 FIVKFLAELPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLRLASPLQG 360
           FIVKFLAELPHIY  VYTEQMEIA+ KA GE LKWEDIKKMKYSWNV CEVLRLASP QG
Sbjct: 301 FIVKFLAELPHIYHGVYTEQMEIAKGKAPGEALKWEDIKKMKYSWNVVCEVLRLASPXQG 360

Query: 361 AFREALNDFVFNGFFIPKGWKLYWSANSTHKNPEYFPEPYKFEPGRFEGIGPLPYTFVPF 420
           AFREAL DFVFNGF+IPKGWKLYWSANSTHK+PEYFPEPYKFEP RFEG GPLPYTFVPF
Sbjct: 361 AFREALTDFVFNGFYIPKGWKLYWSANSTHKSPEYFPEPYKFEPARFEGNGPLPYTFVPF 420

Query: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLDNENIIVNPMPIPQKGLPVRLFPHQP 480
           GGGPRMCPGKEYAKLEILVFMHNLVKRFKW KLL+ ENIIV+PMPIPQKGLP+RLFPHQP
Sbjct: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWAKLLEKENIIVDPMPIPQKGLPIRLFPHQP 480

Query: 481 PSL 483
            SL
Sbjct: 481 LSL 483

BLAST of Chy2G038770 vs. NCBI nr
Match: XP_022987538.1 (beta-amyrin 28-oxidase [Cucurbita maxima])

HSP 1 Score: 834 bits (2154), Expect = 1.14e-302
Identity = 407/485 (83.92%), Postives = 444/485 (91.55%), Query Frame = 0

Query: 1   MELFLISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGESLEFLSSGWRGH 60
           ME FLISLLIL   F+SLTLF+LF+ HKSLFSYPNTPPGAIGLPI+GESLEFLS+GW+G 
Sbjct: 1   MEDFLISLLILFLSFISLTLFVLFYKHKSLFSYPNTPPGAIGLPIIGESLEFLSTGWKGE 60

Query: 61  PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFPS 120
           PEKFIFDRLNKYKSD+FKTSIVGVPAAIFCGP+CNKFLFSNENKLVT WWPDSVNKIFP+
Sbjct: 61  PEKFIFDRLNKYKSDIFKTSIVGVPAAIFCGPVCNKFLFSNENKLVTSWWPDSVNKIFPA 120

Query: 121 TTQTSSKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWENREEVLVFPLAKSF 180
           +T+ +SKE A +LKKLLP+FLKPEALQRYIG+MDELA+RHF S WENRE VLVFPLAKSF
Sbjct: 121 STEINSKESAIRLKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENREHVLVFPLAKSF 180

Query: 181 TFSLACRLFMSVEDEIHVKRLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVA 240
           TFSLACRLF+S+ED IHV+RLS PF++IAAGIISMPIDLPGTPFNRAIKAS F+RKE+++
Sbjct: 181 TFSLACRLFLSLEDRIHVERLSEPFQNIAAGIISMPIDLPGTPFNRAIKASNFVRKELLS 240

Query: 241 IVRQRKQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACT 300
           IVRQRK DLAEGKA  TQDILSHMLLTCDE G YMNES I D ILGLLIGGHDTAS ACT
Sbjct: 241 IVRQRKSDLAEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHDTASAACT 300

Query: 301 FIVKFLAELPHIYDAVYTEQMEIARAKAE--GETLKWEDIKKMKYSWNVACEVLRLASPL 360
           FIVK+LAELP IYD VY EQMEIA+AKA+  GE LKWEDI+KMKYSWNVACEVLRLASPL
Sbjct: 301 FIVKYLAELPQIYDRVYREQMEIAKAKAKAKGELLKWEDIQKMKYSWNVACEVLRLASPL 360

Query: 361 QGAFREALNDFVFNGFFIPKGWKLYWSANSTHKNPEYFPEPYKFEPGRFEGIGPLPYTFV 420
           QGAFREA+NDFVFNGF IPKGWKLYWS+NSTHKN  YFPEPYKF+P RFEG GP PYTFV
Sbjct: 361 QGAFREAINDFVFNGFNIPKGWKLYWSSNSTHKNHAYFPEPYKFDPSRFEGNGPAPYTFV 420

Query: 421 PFGGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLDNENIIVNPMPIPQKGLPVRLFPH 480
           PFGGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLL+ E IIV+P+PIP+KGLPVRLFPH
Sbjct: 421 PFGGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLEKEKIIVDPLPIPEKGLPVRLFPH 480

Query: 481 QPPSL 483
           +  SL
Sbjct: 481 KTLSL 485

BLAST of Chy2G038770 vs. NCBI nr
Match: XP_023516848.1 (beta-amyrin 28-oxidase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 833 bits (2153), Expect = 1.50e-302
Identity = 400/476 (84.03%), Postives = 438/476 (92.02%), Query Frame = 0

Query: 4   FLISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGESLEFLSSGWRGHPEK 63
           FLISLLIL   F+SLTLF+LF+ HKSLFSYPNTPPGAIGLPI+GESLEFLS+GW+G PEK
Sbjct: 4   FLISLLILFLSFISLTLFVLFYKHKSLFSYPNTPPGAIGLPIIGESLEFLSTGWKGEPEK 63

Query: 64  FIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFPSTTQ 123
           FIFDRLNKYKSD+FKTSIVGVPAAIFCGP CNKFLFSNENKLVT WWPDSVNKIFP++T+
Sbjct: 64  FIFDRLNKYKSDIFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPASTE 123

Query: 124 TSSKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWENREEVLVFPLAKSFTFS 183
            +SKE A +LKKLLP+FLKPEALQRYIG+MDELA+RHF S WENRE+VLVFPLAKSFTFS
Sbjct: 124 INSKESAIRLKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENREQVLVFPLAKSFTFS 183

Query: 184 LACRLFMSVEDEIHVKRLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVAIVR 243
           LACRLF+S+ED IHV+RLS PF++IAAGIISMPIDLPGTPFNRAIKAS F+RKE+++IVR
Sbjct: 184 LACRLFVSLEDRIHVERLSEPFQNIAAGIISMPIDLPGTPFNRAIKASNFVRKELLSIVR 243

Query: 244 QRKQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACTFIV 303
           QRK DLAEGKA  TQDILSHMLLTCDE G YMNES I D ILGLLIGGHDT S ACTFIV
Sbjct: 244 QRKSDLAEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHDTTSAACTFIV 303

Query: 304 KFLAELPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLRLASPLQGAFR 363
           K+LAELP IYD+VY EQMEIA+AK  GE LKWEDI+KMKYSWNVACEVLRLASPLQGAFR
Sbjct: 304 KYLAELPQIYDSVYREQMEIAKAKGAGELLKWEDIQKMKYSWNVACEVLRLASPLQGAFR 363

Query: 364 EALNDFVFNGFFIPKGWKLYWSANSTHKNPEYFPEPYKFEPGRFEGIGPLPYTFVPFGGG 423
           E +NDFVFNGF+IPKGWKLYWS+NSTHKN  YFPEPYKF+P RFEG GP PYTFVPFGGG
Sbjct: 364 EPINDFVFNGFYIPKGWKLYWSSNSTHKNHAYFPEPYKFDPSRFEGNGPAPYTFVPFGGG 423

Query: 424 PRMCPGKEYAKLEILVFMHNLVKRFKWTKLLDNENIIVNPMPIPQKGLPVRLFPHQ 479
           PRMCPGKEYAKLEILVFMHNLVKRFKWTKL+D E IIV+P+PIP+KGLPVRLFPH+
Sbjct: 424 PRMCPGKEYAKLEILVFMHNLVKRFKWTKLMDKEKIIVDPLPIPEKGLPVRLFPHK 479

BLAST of Chy2G038770 vs. TAIR 10
Match: AT5G36110.1 (cytochrome P450, family 716, subfamily A, polypeptide 1 )

HSP 1 Score: 637.5 bits (1643), Expect = 8.7e-183
Identity = 300/479 (62.63%), Postives = 372/479 (77.66%), Query Frame = 0

Query: 3   LFLISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGESLEFLSSGWRGHPE 62
           +++  ++IL    + L+L +L   H S FSYPN PPG  GLP++GES  FLS+G +GHPE
Sbjct: 1   MYMAIMIILFLSSILLSLLLLLRKHLSHFSYPNLPPGNTGLPLIGESFSFLSAGRQGHPE 60

Query: 63  KFIFDRLNKYKSD---VFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFP 122
           KFI DR+ ++ S    VFKT + G P A+  G   NKFLF+NENKLV  WWPDSVNKIFP
Sbjct: 61  KFITDRVRRFSSSSSCVFKTHLFGSPTAVVTGASGNKFLFTNENKLVVSWWPDSVNKIFP 120

Query: 123 STTQTSSKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWENREEVLVFPLAKS 182
           S+ QTSSKEEA+KL+ LL QF+KPEAL+RY+G+MDE+A+RHF + W N+++V+VFPL K 
Sbjct: 121 SSMQTSSKEEARKLRMLLSQFMKPEALRRYVGVMDEIAQRHFETEWANQDQVIVFPLTKK 180

Query: 183 FTFSLACRLFMSVEDEIHVKRLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVV 242
           FTFS+ACR F+S+ED   V++L   F  +A GI S+PIDLPGT FNRAIKAS+ +RKEV 
Sbjct: 181 FTFSIACRSFLSMEDPARVRQLEEQFNTVAVGIFSIPIDLPGTRFNRAIKASRLLRKEVS 240

Query: 243 AIVRQRKQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVAC 302
           AIVRQRK++L  GKAL   DILSHML+   E      + D+ DKI+GLLIGGHDTAS+ C
Sbjct: 241 AIVRQRKEELKAGKALEEHDILSHMLMNIGET----KDEDLADKIIGLLIGGHDTASIVC 300

Query: 303 TFIVKFLAELPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLRLASPLQ 362
           TF+V +LAE PH+Y  V  EQ EI + K E E L+WEDI+KM+YSWNVACEV+R+  PL 
Sbjct: 301 TFVVNYLAEFPHVYQRVLQEQKEILKEKKEKEGLRWEDIEKMRYSWNVACEVMRIVPPLS 360

Query: 363 GAFREALNDFVFNGFFIPKGWKLYWSANSTHKNPEYFPEPYKFEPGRFEGIGPLPYTFVP 422
           G FREA++ F F GF+IPKGWKLYWSA +TH NP+YFPEP +FEP RFEG GP PYT+VP
Sbjct: 361 GTFREAIDHFSFKGFYIPKGWKLYWSATATHMNPDYFPEPERFEPNRFEGSGPKPYTYVP 420

Query: 423 FGGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLDNEN-IIVNPMPIPQKGLPVRLFP 478
           FGGGPRMCPGKEYA+LEIL+FMHNLV RFKW K+  NEN I+V+P+PIP KGLP+R+FP
Sbjct: 421 FGGGPRMCPGKEYARLEILIFMHNLVNRFKWEKVFPNENKIVVDPLPIPDKGLPIRIFP 475

BLAST of Chy2G038770 vs. TAIR 10
Match: AT5G36140.1 (cytochrome P450, family 716, subfamily A, polypeptide 2 )

HSP 1 Score: 361.7 bits (927), Expect = 9.3e-100
Identity = 178/318 (55.97%), Postives = 232/318 (72.96%), Query Frame = 0

Query: 9   LILLFFFLSLTLF-ILFH--NHKSLFSYPNTPPGAIGLPILGESLEFLSSGWRGHPEKFI 68
           L ++F F+S  +F +LF    H S F YPN PPG IG P++GE+L FLS+G +GHPEKF+
Sbjct: 3   LTIIFLFISSIIFPLLFFLGKHLSNFRYPNLPPGKIGFPLIGETLSFLSAGRQGHPEKFV 62

Query: 69  FDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFPSTTQTS 128
            DR+  + S +FKT + G P A+  G   NKFLF+NENKLV  WWPDSVNKIFPS+TQTS
Sbjct: 63  TDRVRHFSSGIFKTHLFGSPFAVVTGASGNKFLFTNENKLVISWWPDSVNKIFPSSTQTS 122

Query: 129 SKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWENREEVLVFPLAKSFTFSLA 188
           SKEEA K + LL   +KPEAL+RY+G+MDE+A++HF + W N+++++VFPL K FTFS+A
Sbjct: 123 SKEEAIKTRMLLMPSMKPEALRRYVGVMDEIAQKHFETEWANQDQLIVFPLTKKFTFSIA 182

Query: 189 CRLFMSVEDEIHVKRLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVAIVRQR 248
           CRLF+S++D   V++L  PF  +  G+ S+PIDLPGT FNRAIKAS+ + KEV  I+RQR
Sbjct: 183 CRLFLSMDDLERVRKLEEPFTTVMTGVFSIPIDLPGTRFNRAIKASRLLSKEVSTIIRQR 242

Query: 249 KQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACTFIVKF 308
           K++L  GK    QDILSHML+   E      + D+ DKI+ LLIGGHDT S+ CTF+V +
Sbjct: 243 KEELKAGKVSVEQDILSHMLMNIGET----KDEDLADKIIALLIGGHDTTSIVCTFVVNY 302

Query: 309 LAELPHIYDAVYTEQMEI 324
           LAE PHIY  V  E M+I
Sbjct: 303 LAEFPHIYQRV-LEGMQI 315

BLAST of Chy2G038770 vs. TAIR 10
Match: AT2G42850.1 (cytochrome P450, family 718 )

HSP 1 Score: 277.3 bits (708), Expect = 2.3e-74
Identity = 158/470 (33.62%), Postives = 254/470 (54.04%), Query Frame = 0

Query: 16  LSLTLFILFHNH-----KSLFSYPNTPPGAIGLPILGESLEFLSSGWRGHP-EKFIFDRL 75
           +S TLF     H     K +       PG +GLP +GE+++F  +       E F+  R+
Sbjct: 20  ISSTLFFFRKKHHRFITKKIQKKKKLLPGEMGLPWIGETMDFYKAQKSNRVFEDFVNPRI 79

Query: 76  NKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWP-DSVNKIFPSTTQTSSKE 135
            K+  ++FKT I+G P  +  G   N+ + SNE  LV   WP  SV  +  +       E
Sbjct: 80  IKH-GNIFKTRIMGSPTIVVNGAEANRLILSNEFSLVVSSWPSSSVQLMGMNCIMAKQGE 139

Query: 136 EAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWENREEVLVFPLAKSFTFSLACRL 195
           + + L+ ++   L    L+  I  + +  + H  + W  +EE+ ++  AK  TF++    
Sbjct: 140 KHRVLRGIVANSLSYIGLESLIPKLCDTVKFHHETEWRGKEEISLYRSAKVLTFTVVFEC 199

Query: 196 FMSVEDEIHVKRLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVAIVRQRKQD 255
              ++ EI +  +   FE +  G+ ++P++ P + F RA KA   I   +V  VR+++++
Sbjct: 200 LYGIKVEIGMLEV---FERVLEGVFALPVEFPCSKFARAKKARLEIETFLVGKVREKRRE 259

Query: 256 LAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACTFIVKFLAE 315
           + +  A      L   L+     GV + E ++ D ++ L+   HDT S A +   K LA+
Sbjct: 260 MEKEGAEKPNTTLFSRLVEELIKGV-ITEEEVVDNMVLLVFAAHDTTSYAMSMTFKMLAQ 319

Query: 316 LPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLRLASPLQGAFREALND 375
            P   D +  E  +I   K EGE L  ED+KKMKYSW V  E +RL+ P+ G+FR+A+ D
Sbjct: 320 HPTCRDTLLQEHAQIKANKGEGEYLTVEDVKKMKYSWQVVRETMRLSPPIFGSFRKAVAD 379

Query: 376 FVFNGFFIPKGWKLYWSANSTHKNPEYFPEPYKFEPGRFEGIGPL-PYTFVPFGGGPRMC 435
             + G+ IPKGWK+ W+   TH NPE F +P  F+P RF+   P+  YT++PFGGGPR+C
Sbjct: 380 IDYGGYTIPKGWKILWTTYGTHYNPEIFQDPMSFDPTRFD--KPIQAYTYLPFGGGPRLC 439

Query: 436 PGKEYAKLEILVFMHNLVKRFKWTKLLDNENIIVNPMPIPQKGLPVRLFP 478
            G + AK+ ILVFMH +V  F W+ +  +E I ++P+P P  G+P+++ P
Sbjct: 440 AGHQLAKISILVFMHFVVTGFDWSLVYPDETISMDPLPFPSLGMPIKISP 482

BLAST of Chy2G038770 vs. TAIR 10
Match: AT5G45340.1 (cytochrome P450, family 707, subfamily A, polypeptide 3 )

HSP 1 Score: 258.8 bits (660), Expect = 8.5e-69
Identity = 153/462 (33.12%), Postives = 249/462 (53.90%), Query Frame = 0

Query: 15  FLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGESLEFLSSGWRGHPEKFIFDRLNKYKS 74
           FL L  FI      S    P  PPG +G P +GE+ +  S      P  F   +  +Y S
Sbjct: 16  FLCLLRFIAGVRRSSSTKLP-LPPGTMGYPYVGETFQLYSQ----DPNVFFAAKQRRYGS 75

Query: 75  DVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIF-PSTTQTSSKEEAKKL 134
            VFKT ++G P  +   P   KF+   ++ L  P +P S  ++           +   KL
Sbjct: 76  -VFKTHVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDYHSKL 135

Query: 135 KKLLPQFLKPEALQRYIGIMDELAERHFNSFWENREEVLVFPLAKSFTFSLACRLFMSVE 194
           +KL+ +   P+A++  +  ++ +A+   NS W+   ++  +   K++TF++A    +  +
Sbjct: 136 RKLVLRAFMPDAIRNMVPHIESIAQESLNS-WDG-TQLNTYQEMKTYTFNVALISILGKD 195

Query: 195 DEIHVKRLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVAIVRQRKQDLAEGK 254
           +  + + L   +  +  G  SMPI+LPGT F++A+KA K + + +  I+ +R+Q+ +   
Sbjct: 196 EVYYREDLKRCYYILEKGYNSMPINLPGTLFHKAMKARKELAQILANILSKRRQNPS--- 255

Query: 255 ALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACTFIVKFLAELPHIY 314
             +  D+L   +    E+   + +  I D I+G++    DT +   T+I+K+LA+ P + 
Sbjct: 256 --SHTDLLGSFM----EDKAGLTDEQIADNIIGVIFAARDTTASVLTWILKYLADNPTVL 315

Query: 315 DAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLRLASPLQGAFREALNDFVFNG 374
           +AV  EQM I + K EGE+L WED KKM  ++ V  E LR A+ L   FREA+ D  + G
Sbjct: 316 EAVTEEQMAIRKDKKEGESLTWEDTKKMPLTYRVIQETLRAATILSFTFREAVEDVEYEG 375

Query: 375 FFIPKGWKLYWSANSTHKNPEYFPEPYKFEPGRFEGIGPLPYTFVPFGGGPRMCPGKEYA 434
           + IPKGWK+     + H N + F +P KF+P RFE + P P TF+PFG G   CPG E A
Sbjct: 376 YLIPKGWKVLPLFRNIHHNADIFSDPGKFDPSRFE-VAPKPNTFMPFGSGIHSCPGNELA 435

Query: 435 KLEILVFMHNLVKRFKWTKLLDNENIIVNPMPIPQKGLPVRL 476
           KLEI V +H+L  +++W+ +  ++ I   P  +PQ GLP+ L
Sbjct: 436 KLEISVLIHHLTTKYRWSIVGPSDGIQYGPFALPQNGLPIAL 459

BLAST of Chy2G038770 vs. TAIR 10
Match: AT2G29090.1 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 255.4 bits (651), Expect = 9.3e-68
Identity = 154/476 (32.35%), Postives = 251/476 (52.73%), Query Frame = 0

Query: 5   LISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGESLEFLSSGWRGHPEKF 64
           LI+L I++   + L  + L    + L      PPG++GLP +GE+L   +     +P  F
Sbjct: 22  LITLTIVVVVVVLLFKWWLHWKEQRL----RLPPGSMGLPYIGETLRLYTE----NPNSF 81

Query: 65  IFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNK-IFPSTTQ 124
              R NKY  D+FKT I+G P  +   P   + +  ++  L  P +P S  + I P    
Sbjct: 82  FATRQNKY-GDIFKTHILGCPCVMISSPEAARMVLVSKAHLFKPTYPPSKERMIGPEALF 141

Query: 125 TSSKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWENREEVLVFPLAKSFTFS 184
                    LK+L+     P AL+  +  ++ L  +  +S W +++ +      K + F 
Sbjct: 142 FHQGPYHSTLKRLVQSSFMPSALRPTVSHIELLVLQTLSS-WTSQKSINTLEYMKRYAFD 201

Query: 185 LA-CRLFMSVEDEIHVKRLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVAIV 244
           +A    F   E+   +  +   ++ +  G  SMP+DLPGT F++++KA   + +E+  ++
Sbjct: 202 VAIMSAFGDKEEPTTIDVIKLLYQRLERGYNSMPLDLPGTLFHKSMKARIELSEELRKVI 261

Query: 245 RQRKQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACTFI 304
            +R+++  E   L     L  +L   D+    +++S I D I+G++    DT +   T++
Sbjct: 262 EKRRENGREEGGL-----LGVLLGAKDQKRNGLSDSQIADNIIGVIFAATDTTASVLTWL 321

Query: 305 VKFLAELPHIYDAVYTEQMEI-ARAKAEGETLKWEDIKKMKYSWNVACEVLRLASPLQGA 364
           +K+L + P++   V  EQ  I  + K E   + WED +KM  +  V  E LR AS L   
Sbjct: 322 LKYLHDHPNLLQEVSREQFSIRQKIKKENRRISWEDTRKMPLTTRVIQETLRAASVLSFT 381

Query: 365 FREALNDFVFNGFFIPKGWKLYWSANSTHKNPEYFPEPYKFEPGRFEGIGPLPYTFVPFG 424
           FREA+ D  ++G+ IPKGWK+       H + E+FP+P KF+P RFE + P PYT++PFG
Sbjct: 382 FREAVQDVEYDGYLIPKGWKVLPLFRRIHHSSEFFPDPEKFDPSRFE-VAPKPYTYMPFG 441

Query: 425 GGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLDNENIIVNPMPIPQKGLPVRLFP 478
            G   CPG E AKLE+L+ +H+L   F+W  + D E I   P P+P+KGLP+R+ P
Sbjct: 442 NGVHSCPGSELAKLEMLILLHHLTTSFRWEVIGDEEGIQYGPFPVPKKGLPIRVTP 481

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F6H9N66.3e-21071.67Beta-amyrin 28-monooxygenase OS=Vitis vinifera OX=29760 GN=CYP716A15 PE=1 SV=1[more]
A5BFI46.3e-21071.88Beta-amyrin 28-monooxygenase OS=Vitis vinifera OX=29760 GN=CYP716A17 PE=1 SV=2[more]
A0A0S2II385.5e-20671.25Beta-amyrin 28-monooxygenase OS=Kalopanax septemlobus OX=228393 GN=CYP716A94 PE=... [more]
Q2MJ207.9e-20571.28Beta-amyrin 28-monooxygenase OS=Medicago truncatula OX=3880 GN=CYP716A12 PE=1 SV... [more]
I7C6E81.4e-20471.10Beta-amyrin 28-monooxygenase OS=Panax ginseng OX=4054 GN=CYP716A52v2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3B8H46.0e-28098.76beta-amyrin 28-oxidase OS=Cucumis melo OX=3656 GN=LOC103487187 PE=3 SV=1[more]
A0A0A0LXN32.5e-27897.52Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G649550 PE=3 SV=1[more]
A0A6J1JJQ71.0e-23983.92beta-amyrin 28-oxidase OS=Cucurbita maxima OX=3661 GN=LOC111485071 PE=3 SV=1[more]
A0A6J1H8T44.3e-23883.54beta-amyrin 28-oxidase OS=Cucurbita moschata OX=3662 GN=LOC111461545 PE=3 SV=1[more]
A0A6J1DZX73.1e-23681.95beta-amyrin 28-oxidase OS=Momordica charantia OX=3673 GN=LOC111024635 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_008443638.10.098.76PREDICTED: beta-amyrin 28-oxidase [Cucumis melo][more]
XP_004139087.10.097.52beta-amyrin 28-monooxygenase [Cucumis sativus] >KGN66615.1 hypothetical protein ... [more]
XP_038877759.10.090.68LOW QUALITY PROTEIN: beta-amyrin 28-monooxygenase-like [Benincasa hispida][more]
XP_022987538.11.14e-30283.92beta-amyrin 28-oxidase [Cucurbita maxima][more]
XP_023516848.11.50e-30284.03beta-amyrin 28-oxidase [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT5G36110.18.7e-18362.63cytochrome P450, family 716, subfamily A, polypeptide 1 [more]
AT5G36140.19.3e-10055.97cytochrome P450, family 716, subfamily A, polypeptide 2 [more]
AT2G42850.12.3e-7433.62cytochrome P450, family 718 [more]
AT5G45340.18.5e-6933.12cytochrome P450, family 707, subfamily A, polypeptide 3 [more]
AT2G29090.19.3e-6832.35cytochrome P450, family 707, subfamily A, polypeptide 2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 417..427
score: 52.97
coord: 427..450
score: 28.06
coord: 385..409
score: 29.26
coord: 279..296
score: 25.02
IPR001128Cytochrome P450PRINTSPR00385P450coord: 418..427
score: 58.31
coord: 290..307
score: 27.64
coord: 427..438
score: 35.55
IPR001128Cytochrome P450PFAMPF00067p450coord: 37..452
e-value: 1.0E-54
score: 186.0
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 27..477
e-value: 8.9E-110
score: 369.5
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 30..477
NoneNo IPR availablePANTHERPTHR24286CYTOCHROME P450 26coord: 10..476
NoneNo IPR availablePANTHERPTHR24286:SF297BETA-AMYRIN 28-OXIDASE-LIKEcoord: 10..476
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 420..429

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy2G038770.1Chy2G038770.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016125 sterol metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen