Chy2G035180 (gene) Cucumber (hystrix) v1

Overview
NameChy2G035180
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionThreonine synthase
LocationchrH02: 9120584 .. 9122182 (-)
RNA-Seq ExpressionChy2G035180
SyntenyChy2G035180
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTACCTCCTCCCTCTTCAACCCCACCATTTCTCTTCCTTCTCGCAACCCCAAGCTCCAATCCACCCCGAAATTCCGCAACCCCATTCTCAGAATCCCTAAAGCTACCTCCGATTCTTCTCCCCCTCCACCCACTAACGGCTCTCCCCCATTGTCCACTCTCAAGAACCGCCGTTCTGCTGACGAGAACATCAAAGATGAAGCCCGTCGCCACCGTGCTGCCTCCGGAGAAGATCGGAGCAACTTCTCGGCCAAGTACGTCCCTTTTAACGCTGGCCCCGACTCCACTGAGTGCTACTCTCTGGACGAGATCGTTTACCGAAGCCGATCTGGTGGGTTACTCGACATTCAACACGACATGGAGGCATTGAAGAAATATGACGGCGCGTATTGGCGAAACCTCTTCGATTCGCGCGTGGGGAAAACTACGTGGCCTTATGGTTCCGGCGTTTGGAGTAAGAAGGAATGGGTGTTGCCGGAGATCGATCCCGACGATATCGTCAGCGCTTTCGAAGGTAACTCGAACTTGTTTTGGGCTGAGCGTTTTGGCAAACAGTTTCTGGGTATGAATGATTTATGGGTCAAACATTGTGGGATTAGCCATACTGGGAGTTTCAAGGATTTGGGTATGACTGTTTTGGTTAGCCAAGTGAATCGGTTAAGGAAATTGAAACGGCCTGTCGTCGGAGTCGGTTGTGCGTCGACCGGCGATACATCGGCTGCATTATCGGCTTACTGTGCTTCTGCAGGGATTCCTTCCATTGTATTCCTTCCAGCAAACAAGATTTCCATGGCACAGCTTGTTCAACCCATTGCTAATGGAGCTTTTGTGTTGAGTATAGACACAGATTTTGATGGCTGTATGAAGTTAATAAGAGAAGTTACAGCAGAATTGCCAATTTATTTAGCTAATTCTTTGAATAGTTTGAGACTTGAAGGACAAAAAACAGCAGCTATTGAAATTCTACAGCAATTTGATTGGGGAGTACCTGAATGGGTGATTGTTCCTGGAGGAAATCTTGGTAACATTTATGCATTTTACAAAGGTTTCAAGATGTGTCAAGAACTTGGCCTTGTTGATAGAATTCCAAGGCTTGTTTGTGCTCAAGCAGCCAATGCAAATCCTTTGTATTTACATTACAAATCTGGTTGGAAAGATTTCAAGGCTGTGAAAGCAAGTTCAACTTTTGCTTCCGCCATTCAGATTGGAGATCCGGTTTCTATAGATCGAGCTGTTTACGCTTTGCAGAATTCAAATGGCATTGTTGAGGAAGCAACCGAGGAGGAACTTATGGATGCAATGGCACAAGCTGATTCTACTGGGATGTTCATTTGTCCTCACACTGGGGTTGCACTAACTGCTTTAATCAAGCTGAGAAACAAAGGGATCATCGGACCAACTGATCGGACAGTGGTGGTGAGTACTGCTCATGGTCTGAAGTTCACACAATCCAAAATTGATTATCATTCCAAGGAAATCCCAGACATGGCTTGCCAATTTGCTAACCCACCTGTTCAAGTGAAGGCTGATTTTGGAAGTGTCATGGATGTACTCAAAGATTATTTACTAAATAGTGCTCCAAAGTCTAAAGTGTAA

mRNA sequence

ATGGCTACCTCCTCCCTCTTCAACCCCACCATTTCTCTTCCTTCTCGCAACCCCAAGCTCCAATCCACCCCGAAATTCCGCAACCCCATTCTCAGAATCCCTAAAGCTACCTCCGATTCTTCTCCCCCTCCACCCACTAACGGCTCTCCCCCATTGTCCACTCTCAAGAACCGCCGTTCTGCTGACGAGAACATCAAAGATGAAGCCCGTCGCCACCGTGCTGCCTCCGGAGAAGATCGGAGCAACTTCTCGGCCAAGTACGTCCCTTTTAACGCTGGCCCCGACTCCACTGAGTGCTACTCTCTGGACGAGATCGTTTACCGAAGCCGATCTGGTGGGTTACTCGACATTCAACACGACATGGAGGCATTGAAGAAATATGACGGCGCGTATTGGCGAAACCTCTTCGATTCGCGCGTGGGGAAAACTACGTGGCCTTATGGTTCCGGCGTTTGGAGTAAGAAGGAATGGGTGTTGCCGGAGATCGATCCCGACGATATCGTCAGCGCTTTCGAAGGTAACTCGAACTTGTTTTGGGCTGAGCGTTTTGGCAAACAGTTTCTGGGTATGAATGATTTATGGGTCAAACATTGTGGGATTAGCCATACTGGGAGTTTCAAGGATTTGGGTATGACTGTTTTGGTTAGCCAAGTGAATCGGTTAAGGAAATTGAAACGGCCTGTCGTCGGAGTCGGTTGTGCGTCGACCGGCGATACATCGGCTGCATTATCGGCTTACTGTGCTTCTGCAGGGATTCCTTCCATTGTATTCCTTCCAGCAAACAAGATTTCCATGGCACAGCTTGTTCAACCCATTGCTAATGGAGCTTTTGTGTTGAGTATAGACACAGATTTTGATGGCTGTATGAAGTTAATAAGAGAAGTTACAGCAGAATTGCCAATTTATTTAGCTAATTCTTTGAATAGTTTGAGACTTGAAGGACAAAAAACAGCAGCTATTGAAATTCTACAGCAATTTGATTGGGGAGTACCTGAATGGGTGATTGTTCCTGGAGGAAATCTTGGTAACATTTATGCATTTTACAAAGGTTTCAAGATGTGTCAAGAACTTGGCCTTGTTGATAGAATTCCAAGGCTTGTTTGTGCTCAAGCAGCCAATGCAAATCCTTTGTATTTACATTACAAATCTGGTTGGAAAGATTTCAAGGCTGTGAAAGCAAGTTCAACTTTTGCTTCCGCCATTCAGATTGGAGATCCGGTTTCTATAGATCGAGCTGTTTACGCTTTGCAGAATTCAAATGGCATTGTTGAGGAAGCAACCGAGGAGGAACTTATGGATGCAATGGCACAAGCTGATTCTACTGGGATGTTCATTTGTCCTCACACTGGGGTTGCACTAACTGCTTTAATCAAGCTGAGAAACAAAGGGATCATCGGACCAACTGATCGGACAGTGGTGGTGAGTACTGCTCATGGTCTGAAGTTCACACAATCCAAAATTGATTATCATTCCAAGGAAATCCCAGACATGGCTTGCCAATTTGCTAACCCACCTGTTCAAGTGAAGGCTGATTTTGGAAGTGTCATGGATGTACTCAAAGATTATTTACTAAATAGTGCTCCAAAGTCTAAAGTGTAA

Coding sequence (CDS)

ATGGCTACCTCCTCCCTCTTCAACCCCACCATTTCTCTTCCTTCTCGCAACCCCAAGCTCCAATCCACCCCGAAATTCCGCAACCCCATTCTCAGAATCCCTAAAGCTACCTCCGATTCTTCTCCCCCTCCACCCACTAACGGCTCTCCCCCATTGTCCACTCTCAAGAACCGCCGTTCTGCTGACGAGAACATCAAAGATGAAGCCCGTCGCCACCGTGCTGCCTCCGGAGAAGATCGGAGCAACTTCTCGGCCAAGTACGTCCCTTTTAACGCTGGCCCCGACTCCACTGAGTGCTACTCTCTGGACGAGATCGTTTACCGAAGCCGATCTGGTGGGTTACTCGACATTCAACACGACATGGAGGCATTGAAGAAATATGACGGCGCGTATTGGCGAAACCTCTTCGATTCGCGCGTGGGGAAAACTACGTGGCCTTATGGTTCCGGCGTTTGGAGTAAGAAGGAATGGGTGTTGCCGGAGATCGATCCCGACGATATCGTCAGCGCTTTCGAAGGTAACTCGAACTTGTTTTGGGCTGAGCGTTTTGGCAAACAGTTTCTGGGTATGAATGATTTATGGGTCAAACATTGTGGGATTAGCCATACTGGGAGTTTCAAGGATTTGGGTATGACTGTTTTGGTTAGCCAAGTGAATCGGTTAAGGAAATTGAAACGGCCTGTCGTCGGAGTCGGTTGTGCGTCGACCGGCGATACATCGGCTGCATTATCGGCTTACTGTGCTTCTGCAGGGATTCCTTCCATTGTATTCCTTCCAGCAAACAAGATTTCCATGGCACAGCTTGTTCAACCCATTGCTAATGGAGCTTTTGTGTTGAGTATAGACACAGATTTTGATGGCTGTATGAAGTTAATAAGAGAAGTTACAGCAGAATTGCCAATTTATTTAGCTAATTCTTTGAATAGTTTGAGACTTGAAGGACAAAAAACAGCAGCTATTGAAATTCTACAGCAATTTGATTGGGGAGTACCTGAATGGGTGATTGTTCCTGGAGGAAATCTTGGTAACATTTATGCATTTTACAAAGGTTTCAAGATGTGTCAAGAACTTGGCCTTGTTGATAGAATTCCAAGGCTTGTTTGTGCTCAAGCAGCCAATGCAAATCCTTTGTATTTACATTACAAATCTGGTTGGAAAGATTTCAAGGCTGTGAAAGCAAGTTCAACTTTTGCTTCCGCCATTCAGATTGGAGATCCGGTTTCTATAGATCGAGCTGTTTACGCTTTGCAGAATTCAAATGGCATTGTTGAGGAAGCAACCGAGGAGGAACTTATGGATGCAATGGCACAAGCTGATTCTACTGGGATGTTCATTTGTCCTCACACTGGGGTTGCACTAACTGCTTTAATCAAGCTGAGAAACAAAGGGATCATCGGACCAACTGATCGGACAGTGGTGGTGAGTACTGCTCATGGTCTGAAGTTCACACAATCCAAAATTGATTATCATTCCAAGGAAATCCCAGACATGGCTTGCCAATTTGCTAACCCACCTGTTCAAGTGAAGGCTGATTTTGGAAGTGTCATGGATGTACTCAAAGATTATTTACTAAATAGTGCTCCAAAGTCTAAAGTGTAA

Protein sequence

MATSSLFNPTISLPSRNPKLQSTPKFRNPILRIPKATSDSSPPPPTNGSPPLSTLKNRRSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGPDSTECYSLDEIVYRSRSGGLLDIQHDMEALKKYDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLFWAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGDTSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAELPIYLANSLNSLRLEGQKTAAIEILQQFDWGVPEWVIVPGGNLGNIYAFYKGFKMCQELGLVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVYALQNSNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNKGIIGPTDRTVVVSTAHGLKFTQSKIDYHSKEIPDMACQFANPPVQVKADFGSVMDVLKDYLLNSAPKSKV*
Homology
BLAST of Chy2G035180 vs. ExPASy Swiss-Prot
Match: Q9MT28 (Threonine synthase, chloroplastic OS=Solanum tuberosum OX=4113 PE=2 SV=1)

HSP 1 Score: 867.8 bits (2241), Expect = 6.2e-251
Identity = 427/489 (87.32%), Postives = 453/489 (92.64%), Query Frame = 0

Query: 41  SPPPPTNGSPPLSTLKNRRSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGPDSTECY 100
           +P   T  +  +S  K+RR ADENI++EARRH ++      NFSA+YVPFNAGP+S E Y
Sbjct: 35  TPIKATATNDAISQQKHRRPADENIREEARRHCSS-----HNFSARYVPFNAGPNSDEWY 94

Query: 101 SLDEIVYRSRSGGLLDIQHDMEALKKYDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLP 160
           SLDEIVYRSRSGGLLD+QHDM+ALKK+DG YWR+LFDSRVGKTTWPYGSGVWSKKEWVLP
Sbjct: 95  SLDEIVYRSRSGGLLDVQHDMDALKKFDGQYWRSLFDSRVGKTTWPYGSGVWSKKEWVLP 154

Query: 161 EIDPDDIVSAFEGNSNLFWAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNR 220
           EID DDIVSAFEGNSNLFWAERFGKQFLGM DLWVKHCGISHTGSFKDLGMTVLVSQVNR
Sbjct: 155 EIDSDDIVSAFEGNSNLFWAERFGKQFLGMTDLWVKHCGISHTGSFKDLGMTVLVSQVNR 214

Query: 221 LRKLKRPVVGVGCASTGDTSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLS 280
           LRK+ +PVVGVGCASTGDTSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLS
Sbjct: 215 LRKMHKPVVGVGCASTGDTSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLS 274

Query: 281 IDTDFDGCMKLIREVTAELPIYLANSLNSLRLEGQKTAAIEILQQFDWGVPEWVIVPGGN 340
           IDTDFDGCM+LIREVTAELPIYLANSLNSLRLEGQKTAAIEILQQFDW VPEWVIVPGGN
Sbjct: 275 IDTDFDGCMQLIREVTAELPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPEWVIVPGGN 334

Query: 341 LGNIYAFYKGFKMCQELGLVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASA 400
           LGNIYAFYKGF+MC+ELGLVDRIPRLVCAQAANANPLYLHYKSGWKDFK VKA++TFASA
Sbjct: 335 LGNIYAFYKGFQMCKELGLVDRIPRLVCAQAANANPLYLHYKSGWKDFKPVKANTTFASA 394

Query: 401 IQIGDPVSIDRAVYALQNSNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLR 460
           IQIGDPVSIDRAV+ALQ  NGIVEEATEEELMDAMAQADSTGMFICPHTGVALTAL KLR
Sbjct: 395 IQIGDPVSIDRAVFALQQCNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALFKLR 454

Query: 461 NKGIIGPTDRTVVVSTAHGLKFTQSKIDYHSKEIPDMACQFANPPVQVKADFGSVMDVLK 520
           N G+I PTDRTVVVSTAHGLKFTQSKIDYHSKEI DM C+FANPPV+VKADFGSVMDVLK
Sbjct: 455 NSGVIAPTDRTVVVSTAHGLKFTQSKIDYHSKEIKDMECRFANPPVEVKADFGSVMDVLK 514

Query: 521 DYLLNSAPK 530
            YLL+   K
Sbjct: 515 SYLLSQNSK 518

BLAST of Chy2G035180 vs. ExPASy Swiss-Prot
Match: Q9S7B5 (Threonine synthase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TS1 PE=1 SV=1)

HSP 1 Score: 864.8 bits (2233), Expect = 5.2e-250
Identity = 434/524 (82.82%), Postives = 467/524 (89.12%), Query Frame = 0

Query: 1   MATSSLFNPTISLPSRNPKLQSTPKFRNPILRIPKATSDSSPPPPTNGSPPLST-LKNRR 60
           MA+S LFN ++S  S NPK     + R+  L   +    S      N   P+ T +K   
Sbjct: 1   MASSCLFNASVS--SLNPKQDPIRRHRSTSLLRHRPVVISCTADGNNIKAPIETAVKPPH 60

Query: 61  SADENIKDEARRHRAASGEDRSNFSAKYVPFNAGPDSTECYSLDEIVYRSRSGGLLDIQH 120
             ++NI+DEARR+R+ +    + FSAKYVPFNA P STE YSLDEIVYRSRSGGLLD++H
Sbjct: 61  RTEDNIRDEARRNRSNA---VNPFSAKYVPFNAAPGSTESYSLDEIVYRSRSGGLLDVEH 120

Query: 121 DMEALKKYDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLFW 180
           DMEALK++DGAYWR+LFDSRVGK+TWPYGSGVWSKKEWVLPEID DDIVSAFEGNSNLFW
Sbjct: 121 DMEALKRFDGAYWRDLFDSRVGKSTWPYGSGVWSKKEWVLPEIDDDDIVSAFEGNSNLFW 180

Query: 181 AERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGDT 240
           AERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRK+KRPVVGVGCASTGDT
Sbjct: 181 AERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMKRPVVGVGCASTGDT 240

Query: 241 SAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAEL 300
           SAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIRE+TAEL
Sbjct: 241 SAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREITAEL 300

Query: 301 PIYLANSLNSLRLEGQKTAAIEILQQFDWGVPEWVIVPGGNLGNIYAFYKGFKMCQELGL 360
           PIYLANSLNSLRLEGQKTAAIEILQQFDW VP+WVIVPGGNLGNIYAFYKGFKMCQELGL
Sbjct: 301 PIYLANSLNSLRLEGQKTAAIEILQQFDWQVPDWVIVPGGNLGNIYAFYKGFKMCQELGL 360

Query: 361 VDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVYALQNS 420
           VDRIPR+VCAQAANANPLYLHYKSGWKDFK + AS+TFASAIQIGDPVSIDRAVYAL+  
Sbjct: 361 VDRIPRMVCAQAANANPLYLHYKSGWKDFKPMTASTTFASAIQIGDPVSIDRAVYALKKC 420

Query: 421 NGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNKGIIGPTDRTVVVSTAHG 480
           NGIVEEATEEELMDAMAQADSTGMFICPHTGVALTAL KLRN+G+I PTDRTVVVSTAHG
Sbjct: 421 NGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALFKLRNQGVIAPTDRTVVVSTAHG 480

Query: 481 LKFTQSKIDYHSKEIPDMACQFANPPVQVKADFGSVMDVLKDYL 524
           LKFTQSKIDYHS  IPDMAC+F+NPPV VKADFG+VMDVLK YL
Sbjct: 481 LKFTQSKIDYHSNAIPDMACRFSNPPVDVKADFGAVMDVLKSYL 519

BLAST of Chy2G035180 vs. ExPASy Swiss-Prot
Match: Q9SSP5 (Threonine synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TS2 PE=1 SV=1)

HSP 1 Score: 818.5 bits (2113), Expect = 4.3e-236
Identity = 404/475 (85.05%), Postives = 431/475 (90.74%), Query Frame = 0

Query: 50  PPLSTLKNRRSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGPDSTECYSLDEIVYRS 109
           PP +  K RRS DENI+DEARR          N SA+YVPFNA P STE YSLDEIVYRS
Sbjct: 41  PPQTPQKPRRSPDENIRDEARRR----PHQLQNLSARYVPFNAPPSSTESYSLDEIVYRS 100

Query: 110 RSGGLLDIQHDMEALKKYDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVS 169
           +SG LLD+QHD  ALK+YDG +WRNLFDSRVGKT WPYGSGVWSKKEWVLPEID DDIVS
Sbjct: 101 QSGALLDVQHDFAALKRYDGEFWRNLFDSRVGKTNWPYGSGVWSKKEWVLPEIDDDDIVS 160

Query: 170 AFEGNSNLFWAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVV 229
           AFEGNSNLFWAERFGKQ+L MNDLWVKHCGISHTGSFKDLGM+VLVSQVNRLRK+ +PV+
Sbjct: 161 AFEGNSNLFWAERFGKQYLQMNDLWVKHCGISHTGSFKDLGMSVLVSQVNRLRKMNKPVI 220

Query: 230 GVGCASTGDTSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCM 289
           GVGCASTGDTSAALSAYCASAGIPSIVFLPA+KISMAQLVQPIANGAFVLSIDTDFDGCM
Sbjct: 221 GVGCASTGDTSAALSAYCASAGIPSIVFLPADKISMAQLVQPIANGAFVLSIDTDFDGCM 280

Query: 290 KLIREVTAELPIYLANSLNSLRLEGQKTAAIEILQQFDWGVPEWVIVPGGNLGNIYAFYK 349
            LIREVTAELPIYLANSLNSLRLEGQKTAAIEILQQF+W VP+WVIVPGGNLGNIYAFYK
Sbjct: 281 HLIREVTAELPIYLANSLNSLRLEGQKTAAIEILQQFNWQVPDWVIVPGGNLGNIYAFYK 340

Query: 350 GFKMCQELGLVDRIPRLVCAQAANANPLYLHYKSGWK-DFKAVKASSTFASAIQIGDPVS 409
           GF MC+ELGLVDRIPRLVCAQAANANPLYLHYKSG+K DF  +KA++TFASAIQIGDPVS
Sbjct: 341 GFHMCKELGLVDRIPRLVCAQAANANPLYLHYKSGFKEDFNPLKANTTFASAIQIGDPVS 400

Query: 410 IDRAVYALQNSNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNKGIIGPT 469
           IDRAVYAL+ SNGIVEEATEEELMDA A ADSTGMFICPHTGVALTAL+KLR  G+IG  
Sbjct: 401 IDRAVYALKKSNGIVEEATEEELMDATALADSTGMFICPHTGVALTALMKLRKSGVIGAN 460

Query: 470 DRTVVVSTAHGLKFTQSKIDYHSKEIPDMACQFANPPVQVKADFGSVMDVLKDYL 524
           DRTVVVSTAHGLKFTQSKIDYHSK I +MAC+ ANPPV+VKA FGSVMDVLK+YL
Sbjct: 461 DRTVVVSTAHGLKFTQSKIDYHSKNIKEMACRLANPPVKVKAKFGSVMDVLKEYL 511

BLAST of Chy2G035180 vs. ExPASy Swiss-Prot
Match: Q58860 (Threonine synthase OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=thrC PE=3 SV=1)

HSP 1 Score: 275.4 bits (703), Expect = 1.4e-72
Identity = 161/423 (38.06%), Postives = 242/423 (57.21%), Query Frame = 0

Query: 100 YSLDEIVYRSRSGGLLDIQHDMEALKKYDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVL 159
           Y +DEI+Y    GGLL+I +D E +K         + + ++ K       GVW   E+ L
Sbjct: 12  YDVDEIIYTCECGGLLEIIYDYEEIKD-------KVSEEKLRKRE----IGVWRYLEY-L 71

Query: 160 PEIDPDDIVSAFEGNSNLFWAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVN 219
           P  D   IVS  EG + L+      K+ LG+ +L+VK+ G + TGSFKD GMTV V++ N
Sbjct: 72  PVKDESKIVSLCEGGTPLYRCNNLEKE-LGIKELYVKNEGANPTGSFKDRGMTVGVTRAN 131

Query: 220 RLRKLKRPVVGVGCASTGDTSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVL 279
            L      V  VGCASTG+TSA+L+AY A +G   IV LP  K+++ +L Q +  GA V+
Sbjct: 132 EL-----GVEVVGCASTGNTSASLAAYSARSGKKCIVLLPEGKVALGKLAQAMFYGAKVI 191

Query: 280 SIDTDFDGCMKLIREVTAELPIYLANSLNSLRLEGQKTAAIEILQQFDWGVPEWVIVPGG 339
            +  +FD  + +++++  E  IYL NS+N  RLEGQKT A EI  Q +W VP+ VIVP G
Sbjct: 192 QVKGNFDDALDMVKQLAKEKLIYLLNSINPFRLEGQKTIAFEICDQLNWQVPDRVIVPVG 251

Query: 340 NLGNIYAFYKGFKMCQELGLVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFAS 399
           N GNI A +KGFK  +  G++D +P++   QA  A P+   ++   KD    K   T A+
Sbjct: 252 NAGNISAIWKGFKEFEITGIIDELPKMTGIQADGAKPIVEAFRKRAKDIIPYKNPETIAT 311

Query: 400 AIQIGDPVSIDRAVYALQNSNGIVEEATEEELMDAM-AQADSTGMFICPHTGVALTALIK 459
           AI+IG+PV+  +A+ A+ +S G  E  T+EE+++A    A   G+F+ P +  ++  L K
Sbjct: 312 AIRIGNPVNAPKALDAIYSSGGYAEAVTDEEIVEAQKLLARKEGIFVEPASASSIAGLKK 371

Query: 460 LRNKGIIGPTDRTVVVSTAHGLKFTQSKIDYHSKEIPDMACQFANPPVQVKADFGSVMDV 519
           L  +GII   +R V ++T HGLK             PD A + +  P++++ D   +  +
Sbjct: 372 LLEEGIIDRDERIVCITTGHGLK------------DPDAAIRASEEPIKIECDMNVLKRI 404

Query: 520 LKD 522
           LK+
Sbjct: 432 LKE 404

BLAST of Chy2G035180 vs. ExPASy Swiss-Prot
Match: Q8TQD4 (Threonine synthase OS=Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A) OX=188937 GN=thrC PE=1 SV=1)

HSP 1 Score: 250.0 bits (637), Expect = 6.1e-65
Identity = 150/391 (38.36%), Postives = 228/391 (58.31%), Query Frame = 0

Query: 100 YSLDEIVYR-SRSGGLLDIQHDMEALKKYDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWV 159
           YS DE++Y  S+  GLLD+ +D  ++K           D    KT  P    VW   + +
Sbjct: 13  YSRDEVIYTCSKCDGLLDVIYDYSSIK----------IDMEKLKTECP---SVWKYAKLL 72

Query: 160 LPEIDPDDIVSAFEGNSNLFWAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQV 219
             E +P   V+  EG + L+  +R  ++ +G+  L+VKH G++ TGSFKD GMTV V+  
Sbjct: 73  PVEREP---VTIQEGGTPLYKCDRLAEK-IGIKKLYVKHEGMNPTGSFKDRGMTVGVT-- 132

Query: 220 NRLRKLKRPVVGVGCASTGDTSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFV 279
              + L+  +  V CASTG+TSAAL+ Y A AGIP +V LPA K+++ ++ Q + +GA V
Sbjct: 133 ---KALELGMNTVACASTGNTSAALAIYGAKAGIPVVVLLPAGKVALGKVAQALMHGAKV 192

Query: 280 LSIDTDFDGCMKLIREVTAELPIYLANSLNSLRLEGQKTAAIEILQQFDWGVPEWVIVPG 339
           LSI  +FD  + L+R + ++  IYL NS+N  RLEGQKT   EI  Q D+ VP+ +++P 
Sbjct: 193 LSIRGNFDDALALVRTLCSQEKIYLLNSINPYRLEGQKTIGFEIADQLDFKVPDRIVLPV 252

Query: 340 GNLGNIYAFYKGFKMCQELGLVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFA 399
           GN GNI A YKGF+  + LG+ D +P++   QA  + P+    KSG       +   T A
Sbjct: 253 GNAGNITAIYKGFREFKILGITDSLPKMTGIQAEGSCPIVKAIKSGAPAITPEENPETVA 312

Query: 400 SAIQIGDPVSIDRAVYALQNSNGIVEEATEEELMDAMAQ-ADSTGMFICPHTGVALTALI 459
           +AI+IG+PV+  +A+ A++ S G  E  T+EE++ A    A   G+ + P +  ++  L 
Sbjct: 313 TAIRIGNPVNATKALSAIRESGGTAESVTDEEILAAQKDLARLEGIGVEPASAASVAGLR 372

Query: 460 KLRNKGIIGPTDRTVVVSTAHGLKFTQSKID 489
           KL + G+IG  +  V ++T H LK  Q+ ID
Sbjct: 373 KLVDMGVIGRDETVVCITTGHLLKDPQTVID 381

BLAST of Chy2G035180 vs. ExPASy TrEMBL
Match: A0A0A0M223 (Threonine synthase OS=Cucumis sativus OX=3659 GN=Csa_1G590270 PE=3 SV=1)

HSP 1 Score: 1072.4 bits (2772), Expect = 5.9e-310
Identity = 531/532 (99.81%), Postives = 531/532 (99.81%), Query Frame = 0

Query: 1   MATSSLFNPTISLPSRNPKLQSTPKFRNPILRIPKATSDSSPPPPTNGSPPLSTLKNRRS 60
           MATSSLFNPTISLPSRNPKLQSTPKFRNPILRIPKATSDSSP PPTNGSPPLSTLKNRRS
Sbjct: 1   MATSSLFNPTISLPSRNPKLQSTPKFRNPILRIPKATSDSSPSPPTNGSPPLSTLKNRRS 60

Query: 61  ADENIKDEARRHRAASGEDRSNFSAKYVPFNAGPDSTECYSLDEIVYRSRSGGLLDIQHD 120
           ADENIKDEARRHRAASGEDRSNFSAKYVPFNAGPDSTECYSLDEIVYRSRSGGLLDIQHD
Sbjct: 61  ADENIKDEARRHRAASGEDRSNFSAKYVPFNAGPDSTECYSLDEIVYRSRSGGLLDIQHD 120

Query: 121 MEALKKYDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLFWA 180
           MEALKKYDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLFWA
Sbjct: 121 MEALKKYDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLFWA 180

Query: 181 ERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGDTS 240
           ERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGDTS
Sbjct: 181 ERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGDTS 240

Query: 241 AALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAELP 300
           AALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAELP
Sbjct: 241 AALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAELP 300

Query: 301 IYLANSLNSLRLEGQKTAAIEILQQFDWGVPEWVIVPGGNLGNIYAFYKGFKMCQELGLV 360
           IYLANSLNSLRLEGQKTAAIEILQQFDWGVPEWVIVPGGNLGNIYAFYKGFKMCQELGLV
Sbjct: 301 IYLANSLNSLRLEGQKTAAIEILQQFDWGVPEWVIVPGGNLGNIYAFYKGFKMCQELGLV 360

Query: 361 DRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVYALQNSN 420
           DRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVYALQNSN
Sbjct: 361 DRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVYALQNSN 420

Query: 421 GIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNKGIIGPTDRTVVVSTAHGL 480
           GIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNKGIIGPTDRTVVVSTAHGL
Sbjct: 421 GIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNKGIIGPTDRTVVVSTAHGL 480

Query: 481 KFTQSKIDYHSKEIPDMACQFANPPVQVKADFGSVMDVLKDYLLNSAPKSKV 533
           KFTQSKIDYHSKEIPDMACQFANPPVQVKADFGSVMDVLKDYLLNSAPKSKV
Sbjct: 481 KFTQSKIDYHSKEIPDMACQFANPPVQVKADFGSVMDVLKDYLLNSAPKSKV 532

BLAST of Chy2G035180 vs. ExPASy TrEMBL
Match: A0A5D3CDZ3 (Threonine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G001840 PE=3 SV=1)

HSP 1 Score: 1067.0 bits (2758), Expect = 2.6e-308
Identity = 527/532 (99.06%), Postives = 528/532 (99.25%), Query Frame = 0

Query: 1   MATSSLFNPTISLPSRNPKLQSTPKFRNPILRIPKATSDSSPPPPTNGSPPLSTLKNRRS 60
           MATSSLFNPTIS PSRNPKLQSTPKFRNPILRIPKATSDSSP PPTNGSPPLSTLKNRRS
Sbjct: 1   MATSSLFNPTISFPSRNPKLQSTPKFRNPILRIPKATSDSSPSPPTNGSPPLSTLKNRRS 60

Query: 61  ADENIKDEARRHRAASGEDRSNFSAKYVPFNAGPDSTECYSLDEIVYRSRSGGLLDIQHD 120
           ADENIKDEARRHRAASGEDRSNFSAKYVPFNAGPDSTECYSLDEIVYRSRSGGLLDIQHD
Sbjct: 61  ADENIKDEARRHRAASGEDRSNFSAKYVPFNAGPDSTECYSLDEIVYRSRSGGLLDIQHD 120

Query: 121 MEALKKYDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLFWA 180
           MEALKKYDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLFWA
Sbjct: 121 MEALKKYDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLFWA 180

Query: 181 ERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGDTS 240
           ERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGDTS
Sbjct: 181 ERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGDTS 240

Query: 241 AALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAELP 300
           AALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAELP
Sbjct: 241 AALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAELP 300

Query: 301 IYLANSLNSLRLEGQKTAAIEILQQFDWGVPEWVIVPGGNLGNIYAFYKGFKMCQELGLV 360
           IYLANSLNSLRLEGQKTAAIEILQQFDWGVPEWVIVPGGNLGNIYAFYKGFKMCQELGLV
Sbjct: 301 IYLANSLNSLRLEGQKTAAIEILQQFDWGVPEWVIVPGGNLGNIYAFYKGFKMCQELGLV 360

Query: 361 DRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVYALQNSN 420
           DRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRA+YALQNSN
Sbjct: 361 DRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAIYALQNSN 420

Query: 421 GIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNKGIIGPTDRTVVVSTAHGL 480
           GIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNKGIIGPTDRTVVVSTAHGL
Sbjct: 421 GIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNKGIIGPTDRTVVVSTAHGL 480

Query: 481 KFTQSKIDYHSKEIPDMACQFANPPVQVKADFGSVMDVLKDYLLNSAPKSKV 533
           KF QSKIDYHSKEIPDMACQFANPPVQVKADFGSVMDVLKDYLLN APKSKV
Sbjct: 481 KFAQSKIDYHSKEIPDMACQFANPPVQVKADFGSVMDVLKDYLLNKAPKSKV 532

BLAST of Chy2G035180 vs. ExPASy TrEMBL
Match: A0A5A7UKY2 (Threonine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold181G001890 PE=3 SV=1)

HSP 1 Score: 1065.1 bits (2753), Expect = 9.7e-308
Identity = 527/532 (99.06%), Postives = 527/532 (99.06%), Query Frame = 0

Query: 1   MATSSLFNPTISLPSRNPKLQSTPKFRNPILRIPKATSDSSPPPPTNGSPPLSTLKNRRS 60
           MAT SLFNPTIS PSRNPKLQSTPKFRNPILRIPKATSDSSP PPTNGSPPLSTLKNRRS
Sbjct: 1   MATYSLFNPTISFPSRNPKLQSTPKFRNPILRIPKATSDSSPSPPTNGSPPLSTLKNRRS 60

Query: 61  ADENIKDEARRHRAASGEDRSNFSAKYVPFNAGPDSTECYSLDEIVYRSRSGGLLDIQHD 120
           ADENIKDEARRHRAASGEDRSNFSAKYVPFNAGPDSTECYSLDEIVYRSRSGGLLDIQHD
Sbjct: 61  ADENIKDEARRHRAASGEDRSNFSAKYVPFNAGPDSTECYSLDEIVYRSRSGGLLDIQHD 120

Query: 121 MEALKKYDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLFWA 180
           MEALKKYDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLFWA
Sbjct: 121 MEALKKYDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLFWA 180

Query: 181 ERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGDTS 240
           ERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGDTS
Sbjct: 181 ERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGDTS 240

Query: 241 AALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAELP 300
           AALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAELP
Sbjct: 241 AALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAELP 300

Query: 301 IYLANSLNSLRLEGQKTAAIEILQQFDWGVPEWVIVPGGNLGNIYAFYKGFKMCQELGLV 360
           IYLANSLNSLRLEGQKTAAIEILQQFDWGVPEWVIVPGGNLGNIYAFYKGFKMCQELGLV
Sbjct: 301 IYLANSLNSLRLEGQKTAAIEILQQFDWGVPEWVIVPGGNLGNIYAFYKGFKMCQELGLV 360

Query: 361 DRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVYALQNSN 420
           DRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVYALQNSN
Sbjct: 361 DRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVYALQNSN 420

Query: 421 GIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNKGIIGPTDRTVVVSTAHGL 480
           GIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNKGIIGPTDRTVVVSTAHGL
Sbjct: 421 GIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNKGIIGPTDRTVVVSTAHGL 480

Query: 481 KFTQSKIDYHSKEIPDMACQFANPPVQVKADFGSVMDVLKDYLLNSAPKSKV 533
           KF QSKIDYHSKEIPDMACQFANPPVQVKADFGSVMDVLKDYLLN APKSKV
Sbjct: 481 KFAQSKIDYHSKEIPDMACQFANPPVQVKADFGSVMDVLKDYLLNKAPKSKV 532

BLAST of Chy2G035180 vs. ExPASy TrEMBL
Match: A0A6J1JK49 (Threonine synthase OS=Cucurbita maxima OX=3661 GN=LOC111485156 PE=3 SV=1)

HSP 1 Score: 1007.7 bits (2604), Expect = 1.8e-290
Identity = 501/534 (93.82%), Postives = 510/534 (95.51%), Query Frame = 0

Query: 1   MATSSLFNPTISLPSRNPKLQSTPKFRNPILRI--PKATSDSSPPPPTNGSPPLSTLKNR 60
           MA SSL NP IS PS  PKLQ+ P FRNPILRI  P ATSDSS   PTNGSP LST KNR
Sbjct: 1   MAASSLLNPAISFPSYKPKLQANPNFRNPILRIRRPSATSDSSSSTPTNGSPSLSTTKNR 60

Query: 61  RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGPDSTECYSLDEIVYRSRSGGLLDIQ 120
           RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAG DS+E YSLDE+VYRSRSGGLLD+Q
Sbjct: 61  RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQ 120

Query: 121 HDMEALKKYDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF 180
           HDMEALKK+DGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF
Sbjct: 121 HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF 180

Query: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD 240
           WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD
Sbjct: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD 240

Query: 241 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE 300
           TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE
Sbjct: 241 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE 300

Query: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWGVPEWVIVPGGNLGNIYAFYKGFKMCQELG 360
           LPIYLANSLNSLRLEGQKTAAIEILQQFDW VPEWVI+PGGNLGNIYAFYKGFKMCQELG
Sbjct: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELG 360

Query: 361 LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVYALQN 420
           LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAV ALQN
Sbjct: 361 LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQN 420

Query: 421 SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNKGIIGPTDRTVVVSTAH 480
           SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRN+G+I PTDRTVVVSTAH
Sbjct: 421 SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAH 480

Query: 481 GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADFGSVMDVLKDYLLNSAPKSKV 533
           GLKFTQSKIDYHSKEIPDMACQFANPPVQVKAD+GSVMDVLKDYLL  AP SKV
Sbjct: 481 GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYLLQKAPMSKV 534

BLAST of Chy2G035180 vs. ExPASy TrEMBL
Match: A0A6J1HAA4 (Threonine synthase OS=Cucurbita moschata OX=3662 GN=LOC111461551 PE=3 SV=1)

HSP 1 Score: 1005.7 bits (2599), Expect = 7.0e-290
Identity = 500/534 (93.63%), Postives = 511/534 (95.69%), Query Frame = 0

Query: 1   MATSSLFNPTISLPSRNPKLQSTPKFRNPILRIPK--ATSDSSPPPPTNGSPPLSTLKNR 60
           MA SSL NP IS PS  PKLQ+ PKFRNPILRI +  ATSDSS   PTNGSP LST KNR
Sbjct: 1   MAASSLLNPAISFPSYKPKLQANPKFRNPILRIGRVSATSDSSSSTPTNGSPSLSTTKNR 60

Query: 61  RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGPDSTECYSLDEIVYRSRSGGLLDIQ 120
           RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAG DS+E YSLDE+VYRSRSGGLLD+Q
Sbjct: 61  RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQ 120

Query: 121 HDMEALKKYDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF 180
           HDMEALKK+DGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF
Sbjct: 121 HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF 180

Query: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD 240
           WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD
Sbjct: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD 240

Query: 241 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE 300
           TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE
Sbjct: 241 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE 300

Query: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWGVPEWVIVPGGNLGNIYAFYKGFKMCQELG 360
           LPIYLANSLNSLRLEGQKTAAIEILQQFDW VPEWVI+PGGNLGNIYAFYKGFKMCQELG
Sbjct: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELG 360

Query: 361 LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVYALQN 420
           LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAV ALQN
Sbjct: 361 LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQN 420

Query: 421 SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNKGIIGPTDRTVVVSTAH 480
           SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRN+G+I PTDRTVVVSTAH
Sbjct: 421 SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAH 480

Query: 481 GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADFGSVMDVLKDYLLNSAPKSKV 533
           GLKFTQSKIDYHSKEIPDMACQFANPPVQVKAD+GSVMDVLKDYLL  +P SKV
Sbjct: 481 GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYLLQKSPMSKV 534

BLAST of Chy2G035180 vs. NCBI nr
Match: XP_011660008.1 (threonine synthase, chloroplastic [Cucumis sativus] >KGN66271.1 hypothetical protein Csa_006965 [Cucumis sativus])

HSP 1 Score: 1070 bits (2767), Expect = 0.0
Identity = 531/532 (99.81%), Postives = 531/532 (99.81%), Query Frame = 0

Query: 1   MATSSLFNPTISLPSRNPKLQSTPKFRNPILRIPKATSDSSPPPPTNGSPPLSTLKNRRS 60
           MATSSLFNPTISLPSRNPKLQSTPKFRNPILRIPKATSDSSP PPTNGSPPLSTLKNRRS
Sbjct: 1   MATSSLFNPTISLPSRNPKLQSTPKFRNPILRIPKATSDSSPSPPTNGSPPLSTLKNRRS 60

Query: 61  ADENIKDEARRHRAASGEDRSNFSAKYVPFNAGPDSTECYSLDEIVYRSRSGGLLDIQHD 120
           ADENIKDEARRHRAASGEDRSNFSAKYVPFNAGPDSTECYSLDEIVYRSRSGGLLDIQHD
Sbjct: 61  ADENIKDEARRHRAASGEDRSNFSAKYVPFNAGPDSTECYSLDEIVYRSRSGGLLDIQHD 120

Query: 121 MEALKKYDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLFWA 180
           MEALKKYDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLFWA
Sbjct: 121 MEALKKYDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLFWA 180

Query: 181 ERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGDTS 240
           ERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGDTS
Sbjct: 181 ERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGDTS 240

Query: 241 AALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAELP 300
           AALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAELP
Sbjct: 241 AALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAELP 300

Query: 301 IYLANSLNSLRLEGQKTAAIEILQQFDWGVPEWVIVPGGNLGNIYAFYKGFKMCQELGLV 360
           IYLANSLNSLRLEGQKTAAIEILQQFDWGVPEWVIVPGGNLGNIYAFYKGFKMCQELGLV
Sbjct: 301 IYLANSLNSLRLEGQKTAAIEILQQFDWGVPEWVIVPGGNLGNIYAFYKGFKMCQELGLV 360

Query: 361 DRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVYALQNSN 420
           DRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVYALQNSN
Sbjct: 361 DRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVYALQNSN 420

Query: 421 GIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNKGIIGPTDRTVVVSTAHGL 480
           GIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNKGIIGPTDRTVVVSTAHGL
Sbjct: 421 GIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNKGIIGPTDRTVVVSTAHGL 480

Query: 481 KFTQSKIDYHSKEIPDMACQFANPPVQVKADFGSVMDVLKDYLLNSAPKSKV 532
           KFTQSKIDYHSKEIPDMACQFANPPVQVKADFGSVMDVLKDYLLNSAPKSKV
Sbjct: 481 KFTQSKIDYHSKEIPDMACQFANPPVQVKADFGSVMDVLKDYLLNSAPKSKV 532

BLAST of Chy2G035180 vs. NCBI nr
Match: TYK10051.1 (threonine synthase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 1065 bits (2753), Expect = 0.0
Identity = 527/532 (99.06%), Postives = 528/532 (99.25%), Query Frame = 0

Query: 1   MATSSLFNPTISLPSRNPKLQSTPKFRNPILRIPKATSDSSPPPPTNGSPPLSTLKNRRS 60
           MATSSLFNPTIS PSRNPKLQSTPKFRNPILRIPKATSDSSP PPTNGSPPLSTLKNRRS
Sbjct: 1   MATSSLFNPTISFPSRNPKLQSTPKFRNPILRIPKATSDSSPSPPTNGSPPLSTLKNRRS 60

Query: 61  ADENIKDEARRHRAASGEDRSNFSAKYVPFNAGPDSTECYSLDEIVYRSRSGGLLDIQHD 120
           ADENIKDEARRHRAASGEDRSNFSAKYVPFNAGPDSTECYSLDEIVYRSRSGGLLDIQHD
Sbjct: 61  ADENIKDEARRHRAASGEDRSNFSAKYVPFNAGPDSTECYSLDEIVYRSRSGGLLDIQHD 120

Query: 121 MEALKKYDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLFWA 180
           MEALKKYDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLFWA
Sbjct: 121 MEALKKYDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLFWA 180

Query: 181 ERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGDTS 240
           ERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGDTS
Sbjct: 181 ERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGDTS 240

Query: 241 AALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAELP 300
           AALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAELP
Sbjct: 241 AALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAELP 300

Query: 301 IYLANSLNSLRLEGQKTAAIEILQQFDWGVPEWVIVPGGNLGNIYAFYKGFKMCQELGLV 360
           IYLANSLNSLRLEGQKTAAIEILQQFDWGVPEWVIVPGGNLGNIYAFYKGFKMCQELGLV
Sbjct: 301 IYLANSLNSLRLEGQKTAAIEILQQFDWGVPEWVIVPGGNLGNIYAFYKGFKMCQELGLV 360

Query: 361 DRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVYALQNSN 420
           DRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRA+YALQNSN
Sbjct: 361 DRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAIYALQNSN 420

Query: 421 GIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNKGIIGPTDRTVVVSTAHGL 480
           GIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNKGIIGPTDRTVVVSTAHGL
Sbjct: 421 GIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNKGIIGPTDRTVVVSTAHGL 480

Query: 481 KFTQSKIDYHSKEIPDMACQFANPPVQVKADFGSVMDVLKDYLLNSAPKSKV 532
           KF QSKIDYHSKEIPDMACQFANPPVQVKADFGSVMDVLKDYLLN APKSKV
Sbjct: 481 KFAQSKIDYHSKEIPDMACQFANPPVQVKADFGSVMDVLKDYLLNKAPKSKV 532

BLAST of Chy2G035180 vs. NCBI nr
Match: KAA0055800.1 (threonine synthase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 1063 bits (2748), Expect = 0.0
Identity = 527/532 (99.06%), Postives = 527/532 (99.06%), Query Frame = 0

Query: 1   MATSSLFNPTISLPSRNPKLQSTPKFRNPILRIPKATSDSSPPPPTNGSPPLSTLKNRRS 60
           MAT SLFNPTIS PSRNPKLQSTPKFRNPILRIPKATSDSSP PPTNGSPPLSTLKNRRS
Sbjct: 1   MATYSLFNPTISFPSRNPKLQSTPKFRNPILRIPKATSDSSPSPPTNGSPPLSTLKNRRS 60

Query: 61  ADENIKDEARRHRAASGEDRSNFSAKYVPFNAGPDSTECYSLDEIVYRSRSGGLLDIQHD 120
           ADENIKDEARRHRAASGEDRSNFSAKYVPFNAGPDSTECYSLDEIVYRSRSGGLLDIQHD
Sbjct: 61  ADENIKDEARRHRAASGEDRSNFSAKYVPFNAGPDSTECYSLDEIVYRSRSGGLLDIQHD 120

Query: 121 MEALKKYDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLFWA 180
           MEALKKYDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLFWA
Sbjct: 121 MEALKKYDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLFWA 180

Query: 181 ERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGDTS 240
           ERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGDTS
Sbjct: 181 ERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGDTS 240

Query: 241 AALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAELP 300
           AALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAELP
Sbjct: 241 AALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAELP 300

Query: 301 IYLANSLNSLRLEGQKTAAIEILQQFDWGVPEWVIVPGGNLGNIYAFYKGFKMCQELGLV 360
           IYLANSLNSLRLEGQKTAAIEILQQFDWGVPEWVIVPGGNLGNIYAFYKGFKMCQELGLV
Sbjct: 301 IYLANSLNSLRLEGQKTAAIEILQQFDWGVPEWVIVPGGNLGNIYAFYKGFKMCQELGLV 360

Query: 361 DRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVYALQNSN 420
           DRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVYALQNSN
Sbjct: 361 DRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVYALQNSN 420

Query: 421 GIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNKGIIGPTDRTVVVSTAHGL 480
           GIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNKGIIGPTDRTVVVSTAHGL
Sbjct: 421 GIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNKGIIGPTDRTVVVSTAHGL 480

Query: 481 KFTQSKIDYHSKEIPDMACQFANPPVQVKADFGSVMDVLKDYLLNSAPKSKV 532
           KF QSKIDYHSKEIPDMACQFANPPVQVKADFGSVMDVLKDYLLN APKSKV
Sbjct: 481 KFAQSKIDYHSKEIPDMACQFANPPVQVKADFGSVMDVLKDYLLNKAPKSKV 532

BLAST of Chy2G035180 vs. NCBI nr
Match: XP_038878968.1 (threonine synthase, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 1029 bits (2660), Expect = 0.0
Identity = 511/534 (95.69%), Postives = 519/534 (97.19%), Query Frame = 0

Query: 1   MATSSLFNPTISLPSRNPKLQSTPKFRNPILRIPKA--TSDSSPPPPTNGSPPLSTLKNR 60
           MATSSL NP IS PSR PKLQ  PKFRNP+LRIPKA  TSDSS  PPTNGSPPLSTLKNR
Sbjct: 1   MATSSLLNPAISFPSRKPKLQPNPKFRNPVLRIPKASSTSDSSSSPPTNGSPPLSTLKNR 60

Query: 61  RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGPDSTECYSLDEIVYRSRSGGLLDIQ 120
           RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAG DS E YSLDEI+YRSRSGGLLD+Q
Sbjct: 61  RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSPEWYSLDEIIYRSRSGGLLDVQ 120

Query: 121 HDMEALKKYDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF 180
           HDMEALKK+DGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF
Sbjct: 121 HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF 180

Query: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD 240
           WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD
Sbjct: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD 240

Query: 241 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE 300
           TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE
Sbjct: 241 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE 300

Query: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWGVPEWVIVPGGNLGNIYAFYKGFKMCQELG 360
           LPIYLANSLNSLRLEGQKTAAIEILQQFDWGVPEWVIVPGGNLGNIYAFYKGFKMCQELG
Sbjct: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWGVPEWVIVPGGNLGNIYAFYKGFKMCQELG 360

Query: 361 LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVYALQN 420
           LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVYALQN
Sbjct: 361 LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVYALQN 420

Query: 421 SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNKGIIGPTDRTVVVSTAH 480
           SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTAL+KLRNKGII PTDRTVVVSTAH
Sbjct: 421 SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALVKLRNKGIIRPTDRTVVVSTAH 480

Query: 481 GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADFGSVMDVLKDYLLNSAPKSKV 532
           GLKFTQSKIDYHSK+IPDMACQFANPPVQVKAD+GSVMDVLKDYLLN APKS+V
Sbjct: 481 GLKFTQSKIDYHSKDIPDMACQFANPPVQVKADYGSVMDVLKDYLLNKAPKSQV 534

BLAST of Chy2G035180 vs. NCBI nr
Match: XP_022987673.1 (threonine synthase, chloroplastic [Cucurbita maxima])

HSP 1 Score: 1005 bits (2599), Expect = 0.0
Identity = 501/534 (93.82%), Postives = 510/534 (95.51%), Query Frame = 0

Query: 1   MATSSLFNPTISLPSRNPKLQSTPKFRNPILRI--PKATSDSSPPPPTNGSPPLSTLKNR 60
           MA SSL NP IS PS  PKLQ+ P FRNPILRI  P ATSDSS   PTNGSP LST KNR
Sbjct: 1   MAASSLLNPAISFPSYKPKLQANPNFRNPILRIRRPSATSDSSSSTPTNGSPSLSTTKNR 60

Query: 61  RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGPDSTECYSLDEIVYRSRSGGLLDIQ 120
           RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAG DS+E YSLDE+VYRSRSGGLLD+Q
Sbjct: 61  RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQ 120

Query: 121 HDMEALKKYDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF 180
           HDMEALKK+DGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF
Sbjct: 121 HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF 180

Query: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD 240
           WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD
Sbjct: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD 240

Query: 241 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE 300
           TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE
Sbjct: 241 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE 300

Query: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWGVPEWVIVPGGNLGNIYAFYKGFKMCQELG 360
           LPIYLANSLNSLRLEGQKTAAIEILQQFDW VPEWVI+PGGNLGNIYAFYKGFKMCQELG
Sbjct: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELG 360

Query: 361 LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVYALQN 420
           LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAV ALQN
Sbjct: 361 LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQN 420

Query: 421 SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNKGIIGPTDRTVVVSTAH 480
           SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRN+G+I PTDRTVVVSTAH
Sbjct: 421 SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAH 480

Query: 481 GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADFGSVMDVLKDYLLNSAPKSKV 532
           GLKFTQSKIDYHSKEIPDMACQFANPPVQVKAD+GSVMDVLKDYLL  AP SKV
Sbjct: 481 GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYLLQKAPMSKV 534

BLAST of Chy2G035180 vs. TAIR 10
Match: AT4G29840.1 (Pyridoxal-5'-phosphate-dependent enzyme family protein )

HSP 1 Score: 864.8 bits (2233), Expect = 3.7e-251
Identity = 434/524 (82.82%), Postives = 467/524 (89.12%), Query Frame = 0

Query: 1   MATSSLFNPTISLPSRNPKLQSTPKFRNPILRIPKATSDSSPPPPTNGSPPLST-LKNRR 60
           MA+S LFN ++S  S NPK     + R+  L   +    S      N   P+ T +K   
Sbjct: 1   MASSCLFNASVS--SLNPKQDPIRRHRSTSLLRHRPVVISCTADGNNIKAPIETAVKPPH 60

Query: 61  SADENIKDEARRHRAASGEDRSNFSAKYVPFNAGPDSTECYSLDEIVYRSRSGGLLDIQH 120
             ++NI+DEARR+R+ +    + FSAKYVPFNA P STE YSLDEIVYRSRSGGLLD++H
Sbjct: 61  RTEDNIRDEARRNRSNA---VNPFSAKYVPFNAAPGSTESYSLDEIVYRSRSGGLLDVEH 120

Query: 121 DMEALKKYDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLFW 180
           DMEALK++DGAYWR+LFDSRVGK+TWPYGSGVWSKKEWVLPEID DDIVSAFEGNSNLFW
Sbjct: 121 DMEALKRFDGAYWRDLFDSRVGKSTWPYGSGVWSKKEWVLPEIDDDDIVSAFEGNSNLFW 180

Query: 181 AERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGDT 240
           AERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRK+KRPVVGVGCASTGDT
Sbjct: 181 AERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMKRPVVGVGCASTGDT 240

Query: 241 SAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAEL 300
           SAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIRE+TAEL
Sbjct: 241 SAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREITAEL 300

Query: 301 PIYLANSLNSLRLEGQKTAAIEILQQFDWGVPEWVIVPGGNLGNIYAFYKGFKMCQELGL 360
           PIYLANSLNSLRLEGQKTAAIEILQQFDW VP+WVIVPGGNLGNIYAFYKGFKMCQELGL
Sbjct: 301 PIYLANSLNSLRLEGQKTAAIEILQQFDWQVPDWVIVPGGNLGNIYAFYKGFKMCQELGL 360

Query: 361 VDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVYALQNS 420
           VDRIPR+VCAQAANANPLYLHYKSGWKDFK + AS+TFASAIQIGDPVSIDRAVYAL+  
Sbjct: 361 VDRIPRMVCAQAANANPLYLHYKSGWKDFKPMTASTTFASAIQIGDPVSIDRAVYALKKC 420

Query: 421 NGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNKGIIGPTDRTVVVSTAHG 480
           NGIVEEATEEELMDAMAQADSTGMFICPHTGVALTAL KLRN+G+I PTDRTVVVSTAHG
Sbjct: 421 NGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALFKLRNQGVIAPTDRTVVVSTAHG 480

Query: 481 LKFTQSKIDYHSKEIPDMACQFANPPVQVKADFGSVMDVLKDYL 524
           LKFTQSKIDYHS  IPDMAC+F+NPPV VKADFG+VMDVLK YL
Sbjct: 481 LKFTQSKIDYHSNAIPDMACRFSNPPVDVKADFGAVMDVLKSYL 519

BLAST of Chy2G035180 vs. TAIR 10
Match: AT1G72810.1 (Pyridoxal-5'-phosphate-dependent enzyme family protein )

HSP 1 Score: 818.5 bits (2113), Expect = 3.0e-237
Identity = 404/475 (85.05%), Postives = 431/475 (90.74%), Query Frame = 0

Query: 50  PPLSTLKNRRSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGPDSTECYSLDEIVYRS 109
           PP +  K RRS DENI+DEARR          N SA+YVPFNA P STE YSLDEIVYRS
Sbjct: 41  PPQTPQKPRRSPDENIRDEARRR----PHQLQNLSARYVPFNAPPSSTESYSLDEIVYRS 100

Query: 110 RSGGLLDIQHDMEALKKYDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVS 169
           +SG LLD+QHD  ALK+YDG +WRNLFDSRVGKT WPYGSGVWSKKEWVLPEID DDIVS
Sbjct: 101 QSGALLDVQHDFAALKRYDGEFWRNLFDSRVGKTNWPYGSGVWSKKEWVLPEIDDDDIVS 160

Query: 170 AFEGNSNLFWAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVV 229
           AFEGNSNLFWAERFGKQ+L MNDLWVKHCGISHTGSFKDLGM+VLVSQVNRLRK+ +PV+
Sbjct: 161 AFEGNSNLFWAERFGKQYLQMNDLWVKHCGISHTGSFKDLGMSVLVSQVNRLRKMNKPVI 220

Query: 230 GVGCASTGDTSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCM 289
           GVGCASTGDTSAALSAYCASAGIPSIVFLPA+KISMAQLVQPIANGAFVLSIDTDFDGCM
Sbjct: 221 GVGCASTGDTSAALSAYCASAGIPSIVFLPADKISMAQLVQPIANGAFVLSIDTDFDGCM 280

Query: 290 KLIREVTAELPIYLANSLNSLRLEGQKTAAIEILQQFDWGVPEWVIVPGGNLGNIYAFYK 349
            LIREVTAELPIYLANSLNSLRLEGQKTAAIEILQQF+W VP+WVIVPGGNLGNIYAFYK
Sbjct: 281 HLIREVTAELPIYLANSLNSLRLEGQKTAAIEILQQFNWQVPDWVIVPGGNLGNIYAFYK 340

Query: 350 GFKMCQELGLVDRIPRLVCAQAANANPLYLHYKSGWK-DFKAVKASSTFASAIQIGDPVS 409
           GF MC+ELGLVDRIPRLVCAQAANANPLYLHYKSG+K DF  +KA++TFASAIQIGDPVS
Sbjct: 341 GFHMCKELGLVDRIPRLVCAQAANANPLYLHYKSGFKEDFNPLKANTTFASAIQIGDPVS 400

Query: 410 IDRAVYALQNSNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNKGIIGPT 469
           IDRAVYAL+ SNGIVEEATEEELMDA A ADSTGMFICPHTGVALTAL+KLR  G+IG  
Sbjct: 401 IDRAVYALKKSNGIVEEATEEELMDATALADSTGMFICPHTGVALTALMKLRKSGVIGAN 460

Query: 470 DRTVVVSTAHGLKFTQSKIDYHSKEIPDMACQFANPPVQVKADFGSVMDVLKDYL 524
           DRTVVVSTAHGLKFTQSKIDYHSK I +MAC+ ANPPV+VKA FGSVMDVLK+YL
Sbjct: 461 DRTVVVSTAHGLKFTQSKIDYHSKNIKEMACRLANPPVKVKAKFGSVMDVLKEYL 511

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9MT286.2e-25187.32Threonine synthase, chloroplastic OS=Solanum tuberosum OX=4113 PE=2 SV=1[more]
Q9S7B55.2e-25082.82Threonine synthase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TS1 PE=1 ... [more]
Q9SSP54.3e-23685.05Threonine synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TS2 PE=1 ... [more]
Q588601.4e-7238.06Threonine synthase OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 266... [more]
Q8TQD46.1e-6538.36Threonine synthase OS=Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 /... [more]
Match NameE-valueIdentityDescription
A0A0A0M2235.9e-31099.81Threonine synthase OS=Cucumis sativus OX=3659 GN=Csa_1G590270 PE=3 SV=1[more]
A0A5D3CDZ32.6e-30899.06Threonine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G00... [more]
A0A5A7UKY29.7e-30899.06Threonine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold181G0... [more]
A0A6J1JK491.8e-29093.82Threonine synthase OS=Cucurbita maxima OX=3661 GN=LOC111485156 PE=3 SV=1[more]
A0A6J1HAA47.0e-29093.63Threonine synthase OS=Cucurbita moschata OX=3662 GN=LOC111461551 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_011660008.10.099.81threonine synthase, chloroplastic [Cucumis sativus] >KGN66271.1 hypothetical pro... [more]
TYK10051.10.099.06threonine synthase 1 [Cucumis melo var. makuwa][more]
KAA0055800.10.099.06threonine synthase 1 [Cucumis melo var. makuwa][more]
XP_038878968.10.095.69threonine synthase, chloroplastic-like [Benincasa hispida][more]
XP_022987673.10.093.82threonine synthase, chloroplastic [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT4G29840.13.7e-25182.82Pyridoxal-5'-phosphate-dependent enzyme family protein [more]
AT1G72810.13.0e-23785.05Pyridoxal-5'-phosphate-dependent enzyme family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036052Tryptophan synthase beta subunit-like PLP-dependent enzymeGENE3D3.40.50.1100coord: 281..528
e-value: 3.8E-72
score: 245.2
IPR036052Tryptophan synthase beta subunit-like PLP-dependent enzymeGENE3D3.40.50.1100coord: 59..280
e-value: 4.8E-64
score: 218.6
IPR036052Tryptophan synthase beta subunit-like PLP-dependent enzymeSUPERFAMILY53686Tryptophan synthase beta subunit-like PLP-dependent enzymescoord: 81..522
IPR001926Pyridoxal-phosphate dependent enzymePFAMPF00291PALPcoord: 170..476
e-value: 8.1E-49
score: 166.6
IPR004450Threonine synthase-likeTIGRFAMTIGR00260TIGR00260coord: 158..481
e-value: 1.7E-60
score: 202.9
NoneNo IPR availablePIRSRPIRSR038945-1PIRSR038945-1coord: 150..462
e-value: 5.2E-70
score: 234.0
NoneNo IPR availablePIRSRPIRSR038945-2PIRSR038945-2coord: 150..462
e-value: 5.2E-70
score: 234.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..24
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..80
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 59..79
NoneNo IPR availablePANTHERPTHR10314:SF176THREONINE SYNTHASE, CHLOROPLASTIC-LIKE ISOFORM X1coord: 34..527
NoneNo IPR availablePANTHERPTHR10314CYSTATHIONINE BETA-SYNTHASEcoord: 34..527
NoneNo IPR availableCDDcd01563Thr-synth_1coord: 152..482
e-value: 4.64899E-160
score: 456.285
IPR000634Serine/threonine dehydratase, pyridoxal-phosphate-binding sitePROSITEPS00165DEHYDRATASE_SER_THRcoord: 197..211

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy2G035180.1Chy2G035180.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019344 cysteine biosynthetic process
biological_process GO:0009088 threonine biosynthetic process
biological_process GO:0006520 cellular amino acid metabolic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0030170 pyridoxal phosphate binding
molecular_function GO:0004795 threonine synthase activity