Chy2G033440 (gene) Cucumber (hystrix) v1

Overview


Name	Chy2G033440
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	Cysteine proteinase inhibitor 5-like
Location	chrH02: 7426232 .. 7426477 (-)
RNA-Seq Expression	Chy2G033440
Synteny	Chy2G033440

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GATCCACAAGATCCAAAAGTGAAAGGAATAGCAGAATGGGCAGTGGAAGAATACAACAAAGGAGGCCATAGCTTGACCCATGTAAGTATATTGAAATGTGAGTATCAAGTGGTGAGTGGAATCAATTGGCGTCTTAAATTGAAGTGTGTAGATCAAAACAATTGTGAGGGAGTCTATGAGACTGTTGTGTGGGAGAAATTGGACGGAAGCCTGGTGCTTAGTTACTTCGTTCATCTCTTGACATGA

mRNA sequence

GATCCACAAGATCCAAAAGTGAAAGGAATAGCAGAATGGGCAGTGGAAGAATACAACAAAGGAGGCCATAGCTTGACCCATGTAAGTATATTGAAATGTGAGTATCAAGTGGTGAGTGGAATCAATTGGCGTCTTAAATTGAAGTGTGTAGATCAAAACAATTGTGAGGGAGTCTATGAGACTGTTGTGTGGGAGAAATTGGACGGAAGCCTGGTGCTTAGTTACTTCGTTCATCTCTTGACATGA

Coding sequence (CDS)

GATCCACAAGATCCAAAAGTGAAAGGAATAGCAGAATGGGCAGTGGAAGAATACAACAAAGGAGGCCATAGCTTGACCCATGTAAGTATATTGAAATGTGAGTATCAAGTGGTGAGTGGAATCAATTGGCGTCTTAAATTGAAGTGTGTAGATCAAAACAATTGTGAGGGAGTCTATGAGACTGTTGTGTGGGAGAAATTGGACGGAAGCCTGGTGCTTAGTTACTTCGTTCATCTCTTGACATGA

Protein sequence

DPQDPKVKGIAEWAVEEYNKGGHSLTHVSILKCEYQVVSGINWRLKLKCVDQNNCEGVYETVVWEKLDGSLVLSYFVHLLT*

Homology

[Close]

BLAST of Chy2G033440 vs. ExPASy Swiss-Prot
Match: Q41916 (Cysteine proteinase inhibitor 5 OS=Arabidopsis thaliana OX=3702 GN=CYS5 PE=2 SV=2)

HSP 1 Score: 52.8 bits (125), Expect = 2.2e-06
Identity = 28/64 (43.75%), Postives = 38/64 (59.38%), Query Frame = 0


Query: 4   DPKVKGIAEWAVEEYNKGGHS-LTHVSILKCEYQVVSGINWRLKLKCVDQNNCEGVYETV 63
           DP+V  I E+AV EYNK   S L   +++  E QVVSG N+RLK+   D +     Y  +
Sbjct: 39  DPQVVEIGEFAVSEYNKRSESGLKFETVVSGETQVVSGTNYRLKVAANDGDGVSKNYLAI 98

Query: 64  VWEK 67
           VW+K
Sbjct: 99  VWDK 102

[Close]

BLAST of Chy2G033440 vs. ExPASy Swiss-Prot
Match: Q10Q47 (Putative cysteine proteinase inhibitor 7 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0210100 PE=3 SV=1)

HSP 1 Score: 49.3 bits (116), Expect = 2.4e-05
Identity = 26/68 (38.24%), Postives = 38/68 (55.88%), Query Frame = 0


Query: 1   DPQDPKVKGIAEWAVEEYNKG--GHSLTHVSILKCEYQVVSGINWRLKLKCVDQNNCEGV 60
           D  DP V+ +  WAV E ++G     LT   +   E QVV+G+N+RL L+       +G 
Sbjct: 34  DINDPHVQELGRWAVAEEDRGVAAGGLTFERVTDGEKQVVAGVNYRLTLEASSSGAKDGR 93

Query: 61  YETVVWEK 67
           YE VV+E+
Sbjct: 94  YEAVVYEQ 101

[Close]

BLAST of Chy2G033440 vs. ExPASy Swiss-Prot
Match: P86472 (Cysteine proteinase inhibitor 1 OS=Actinidia chinensis var. chinensis OX=1590841 GN=CYT1 PE=1 SV=2)

HSP 1 Score: 44.3 bits (103), Expect = 7.8e-04
Identity = 24/62 (38.71%), Postives = 38/62 (61.29%), Query Frame = 0


Query: 6   KVKGIAEWAVEEYNK-GGHSLTHVSILKCEYQVVSGINWRLKLKCVDQNNCEGVYETVVW 65
           +V+ +A++AV E+NK     L + S+++   QVV+G N+RL +   D     G YE VVW
Sbjct: 42  EVQDVAQFAVSEHNKQANDELQYQSVVRGYTQVVAGTNYRLVIAAKD-GAVVGNYEAVVW 101

Query: 66  EK 67
           +K
Sbjct: 102 DK 102

[Close]

BLAST of Chy2G033440 vs. ExPASy Swiss-Prot
Match: Q6TPK4 (Cysteine proteinase inhibitor 1 OS=Actinidia deliciosa OX=3627 PE=1 SV=1)

HSP 1 Score: 44.3 bits (103), Expect = 7.8e-04
Identity = 24/62 (38.71%), Postives = 38/62 (61.29%), Query Frame = 0


Query: 6   KVKGIAEWAVEEYNK-GGHSLTHVSILKCEYQVVSGINWRLKLKCVDQNNCEGVYETVVW 65
           +V+ +A++AV E+NK     L + S+++   QVV+G N+RL +   D     G YE VVW
Sbjct: 42  EVQDVAQFAVSEHNKQANDELQYQSVVRGYTQVVAGTNYRLVIAAKD-GAVVGNYEAVVW 101

Query: 66  EK 67
           +K
Sbjct: 102 DK 102

[Close]

BLAST of Chy2G033440 vs. ExPASy TrEMBL
Match: A0A0A0KHN6 (Cystatin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G482230 PE=4 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 2.4e-24
Identity = 62/81 (76.54%), Postives = 65/81 (80.25%), Query Frame = 0


Query: 1  DPQDPKVKGIAEWAVEEYNKGGHSLTHVSILKCEYQVVSGINWRLKLKCVDQ-NNCEGVY 60
          DP    VK IAEWAVEEYNKG H L+ VSILKCE QVV+G NWRLKLKCVDQ NN  G+Y
Sbjct: 15 DPNSLHVKRIAEWAVEEYNKGDHFLSLVSILKCESQVVNGTNWRLKLKCVDQINNRLGIY 74

Query: 61 ETVVWEKLDGSLVLSYFVHLL 81
           TVVWEKLDGSLVLS FV LL
Sbjct: 75 VTVVWEKLDGSLVLSDFVPLL 95

[Close]

BLAST of Chy2G033440 vs. ExPASy TrEMBL
Match: O80389 (Cystein proteinase inhibitor OS=Cucumis sativus OX=3659 PE=2 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 1.9e-21
Identity = 57/83 (68.67%), Postives = 62/83 (74.70%), Query Frame = 0


Query: 1  DPQDPKVKGIAEWAVEEYNKG-GHSLTHVSILKCEYQVVSGINWRLKLKCVDQNNCEGVY 60
          DP DP VK IAEWAV EYNK  GH LT VSILKCE QVV+G+NWRL LKC D+NN EG Y
Sbjct: 14 DPNDPHVKDIAEWAVAEYNKSQGHHLTLVSILKCESQVVAGVNWRLVLKCKDENNGEGNY 73

Query: 61 ETVVWEKL-DGSLVLSYFVHLLT 82
          ETVVWEK+ +    L  F HLLT
Sbjct: 74 ETVVWEKIWENFRQLITFDHLLT 96

[Close]

BLAST of Chy2G033440 vs. ExPASy TrEMBL
Match: A0A5A7TBL3 (Cysteine proteinase inhibitor 5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold529G00310 PE=4 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 2.2e-17
Identity = 48/82 (58.54%), Postives = 56/82 (68.29%), Query Frame = 0


Query: 1  DPQDPKVKGIAEWAVEEYNKGGHSLTHVSILKCEYQVVSGINWRLKLKCVDQNNCEGVYE 60
          + Q    K + +WAV E+NK GH LT VS+LKCE QVV G NWRL +KC D+NN EG YE
Sbjct: 13 ESQSEHAKKLCQWAVAEHNKQGHHLTFVSLLKCESQVVDGTNWRLVMKCKDENNYEGNYE 72

Query: 61 TVVWEK-LDGSLVLSYFVHLLT 82
           VVWEK L+G L L  F  LLT
Sbjct: 73 AVVWEKILEGFLQLISFFPLLT 94

[Close]

BLAST of Chy2G033440 vs. ExPASy TrEMBL
Match: A0A5A7TGL3 (Cysteine proteinase inhibitor 5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold529G00530 PE=4 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 7.3e-13
Identity = 45/83 (54.22%), Postives = 54/83 (65.06%), Query Frame = 0


Query: 2  PQDPKVKGIAEWAVEEYNKGGHSLTHVSILKCEYQVVSGINWRLKLKCVDQNNCEG-VYE 61
          P D +V+ IAEWAV++YN+ GH LT +  L CE QVV G NWRL LKC D+NN E   Y+
Sbjct: 15 PDDEEVQEIAEWAVKKYNEKGHHLTLLRKLDCESQVVGGTNWRLLLKCRDENNGENHYYQ 74

Query: 62 TVV----WEKLDGSLVLSYFVHL 80
          TVV    WE L G   L+YF  L
Sbjct: 75 TVVYVRPWEHLTG---LTYFYRL 94

[Close]

BLAST of Chy2G033440 vs. ExPASy TrEMBL
Match: A0A5A7SLP1 (Cysteine proteinase inhibitor 5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold541G00100 PE=4 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 3.6e-12
Identity = 44/83 (53.01%), Postives = 54/83 (65.06%), Query Frame = 0


Query: 2  PQDPKVKGIAEWAVEEYNKGGHSLTHVSILKCEYQVVSGINWRLKLKCVDQNNCEG-VYE 61
          P D +V+ IAEWAV++YN+ GH LT +  L CE QVV+G NWRL LKC D+NN E   Y+
Sbjct: 15 PDDEEVQEIAEWAVKKYNEKGHHLTLLRKLDCESQVVAGTNWRLLLKCRDENNGENHYYQ 74

Query: 62 TVV----WEKLDGSLVLSYFVHL 80
          TVV    WE L     L+YF  L
Sbjct: 75 TVVYVRPWEHL---TALTYFYRL 94

[Close]

BLAST of Chy2G033440 vs. NCBI nr
Match: KAE8647451.1 (hypothetical protein Csa_002769 [Cucumis sativus])

HSP 1 Score: 112 bits (281), Expect = 1.82e-29
Identity = 56/75 (74.67%), Postives = 60/75 (80.00%), Query Frame = 0


Query: 1  DPQDPKVKGIAEWAVEEYNKGGHSLTHVSILKCEYQVVSGINWRLKLKCVDQ-NNCEGVY 60
          DP    VK IAEWAVEEYNKG H L+ VSILKCE QVV+G NWRLKLKCVDQ NN  G+Y
Sbjct: 15 DPNSLHVKRIAEWAVEEYNKGDHFLSLVSILKCESQVVNGTNWRLKLKCVDQINNRLGIY 74

Query: 61 ETVVWEKLDGSLVLS 74
           TVVWEKLDGSL +S
Sbjct: 75 VTVVWEKLDGSLCMS 89

[Close]

BLAST of Chy2G033440 vs. NCBI nr
Match: NP_001267677.1 (cysteine proteinase inhibitor 5-like [Cucumis sativus] >BAA28867.1 cystein proteinase inhibitor [Cucumis sativus])

HSP 1 Score: 110 bits (275), Expect = 2.07e-29
Identity = 57/83 (68.67%), Postives = 62/83 (74.70%), Query Frame = 0


Query: 1  DPQDPKVKGIAEWAVEEYNKG-GHSLTHVSILKCEYQVVSGINWRLKLKCVDQNNCEGVY 60
          DP DP VK IAEWAV EYNK  GH LT VSILKCE QVV+G+NWRL LKC D+NN EG Y
Sbjct: 14 DPNDPHVKDIAEWAVAEYNKSQGHHLTLVSILKCESQVVAGVNWRLVLKCKDENNGEGNY 73

Query: 61 ETVVWEKL-DGSLVLSYFVHLLT 81
          ETVVWEK+ +    L  F HLLT
Sbjct: 74 ETVVWEKIWENFRQLITFDHLLT 96

[Close]

BLAST of Chy2G033440 vs. NCBI nr
Match: KAE8647756.1 (hypothetical protein Csa_003034 [Cucumis sativus])

HSP 1 Score: 110 bits (275), Expect = 4.00e-27
Identity = 57/83 (68.67%), Postives = 62/83 (74.70%), Query Frame = 0


Query: 1   DPQDPKVKGIAEWAVEEYNKG-GHSLTHVSILKCEYQVVSGINWRLKLKCVDQNNCEGVY 60
           DP DP VK IAEWAV EYNK  GH LT VSILKCE QVV+G+NWRL LKC D+NN EG Y
Sbjct: 227 DPNDPHVKDIAEWAVAEYNKSQGHHLTLVSILKCESQVVAGVNWRLVLKCKDENNGEGNY 286

Query: 61  ETVVWEKL-DGSLVLSYFVHLLT 81
           ETVVWEK+ +    L  F HLLT
Sbjct: 287 ETVVWEKIWENFRQLITFDHLLT 309

[Close]

BLAST of Chy2G033440 vs. NCBI nr
Match: KAA0040710.1 (cysteine proteinase inhibitor 5-like [Cucumis melo var. makuwa])

HSP 1 Score: 97.1 bits (240), Expect = 4.18e-24
Identity = 48/82 (58.54%), Postives = 56/82 (68.29%), Query Frame = 0


Query: 1  DPQDPKVKGIAEWAVEEYNKGGHSLTHVSILKCEYQVVSGINWRLKLKCVDQNNCEGVYE 60
          + Q    K + +WAV E+NK GH LT VS+LKCE QVV G NWRL +KC D+NN EG YE
Sbjct: 13 ESQSEHAKKLCQWAVAEHNKQGHHLTFVSLLKCESQVVDGTNWRLVMKCKDENNYEGNYE 72

Query: 61 TVVWEK-LDGSLVLSYFVHLLT 81
           VVWEK L+G L L  F  LLT
Sbjct: 73 AVVWEKILEGFLQLISFFPLLT 94

[Close]

BLAST of Chy2G033440 vs. NCBI nr
Match: KAA0040727.1 (cysteine proteinase inhibitor 5-like [Cucumis melo var. makuwa])

HSP 1 Score: 82.0 bits (201), Expect = 3.98e-18
Identity = 45/83 (54.22%), Postives = 54/83 (65.06%), Query Frame = 0


Query: 2  PQDPKVKGIAEWAVEEYNKGGHSLTHVSILKCEYQVVSGINWRLKLKCVDQNNCEG-VYE 61
          P D +V+ IAEWAV++YN+ GH LT +  L CE QVV G NWRL LKC D+NN E   Y+
Sbjct: 15 PDDEEVQEIAEWAVKKYNEKGHHLTLLRKLDCESQVVGGTNWRLLLKCRDENNGENHYYQ 74

Query: 62 TVV----WEKLDGSLVLSYFVHL 79
          TVV    WE L G   L+YF  L
Sbjct: 75 TVVYVRPWEHLTG---LTYFYRL 94

[Close]

BLAST of Chy2G033440 vs. TAIR 10
Match: AT5G47550.1 (Cystatin/monellin superfamily protein )

HSP 1 Score: 52.8 bits (125), Expect = 1.6e-07
Identity = 28/64 (43.75%), Postives = 38/64 (59.38%), Query Frame = 0


Query: 4   DPKVKGIAEWAVEEYNKGGHS-LTHVSILKCEYQVVSGINWRLKLKCVDQNNCEGVYETV 63
           DP+V  I E+AV EYNK   S L   +++  E QVVSG N+RLK+   D +     Y  +
Sbjct: 39  DPQVVEIGEFAVSEYNKRSESGLKFETVVSGETQVVSGTNYRLKVAANDGDGVSKNYLAI 98

Query: 64  VWEK 67
           VW+K
Sbjct: 99  VWDK 102

The following BLAST results are available for this feature:

BLAST of Chy2G033440 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q41916	2.2e-06	43.75	Cysteine proteinase inhibitor 5 OS=Arabidopsis thaliana OX=3702 GN=CYS5 PE=2 SV=...	[more]
Q10Q47	2.4e-05	38.24	Putative cysteine proteinase inhibitor 7 OS=Oryza sativa subsp. japonica OX=3994...	[more]
P86472	7.8e-04	38.71	Cysteine proteinase inhibitor 1 OS=Actinidia chinensis var. chinensis OX=1590841...	[more]
Q6TPK4	7.8e-04	38.71	Cysteine proteinase inhibitor 1 OS=Actinidia deliciosa OX=3627 PE=1 SV=1	[more]

BLAST of Chy2G033440 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KHN6	2.4e-24	76.54	Cystatin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G482230 PE...	[more]
O80389	1.9e-21	68.67	Cystein proteinase inhibitor OS=Cucumis sativus OX=3659 PE=2 SV=1	[more]
A0A5A7TBL3	2.2e-17	58.54	Cysteine proteinase inhibitor 5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E...	[more]
A0A5A7TGL3	7.3e-13	54.22	Cysteine proteinase inhibitor 5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E...	[more]
A0A5A7SLP1	3.6e-12	53.01	Cysteine proteinase inhibitor 5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E...	[more]

BLAST of Chy2G033440 vs. NCBI nr


Match Name	E-value	Identity	Description
KAE8647451.1	1.82e-29	74.67	hypothetical protein Csa_002769 [Cucumis sativus]	[more]
NP_001267677.1	2.07e-29	68.67	cysteine proteinase inhibitor 5-like [Cucumis sativus] >BAA28867.1 cystein prote...	[more]
KAE8647756.1	4.00e-27	68.67	hypothetical protein Csa_003034 [Cucumis sativus]	[more]
KAA0040710.1	4.18e-24	58.54	cysteine proteinase inhibitor 5-like [Cucumis melo var. makuwa]	[more]
KAA0040727.1	3.98e-18	54.22	cysteine proteinase inhibitor 5-like [Cucumis melo var. makuwa]	[more]

BLAST of Chy2G033440 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G47550.1	1.6e-07	43.75	Cystatin/monellin superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.10.450.10		coord: 1..79 e-value: 6.6E-17 score: 63.4
None	No IPR available	PANTHER	PTHR47364	CYSTEINE PROTEINASE INHIBITOR 5	coord: 1..66
None	No IPR available	PANTHER	PTHR47364:SF2	CYSTEINE PROTEINASE INHIBITOR 5	coord: 1..66
None	No IPR available	SUPERFAMILY	54403	Cystatin/monellin	coord: 1..69
IPR000010	Cystatin domain	PFAM	PF16845	SQAPI	coord: 3..68 e-value: 4.3E-17 score: 62.2

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy2G033440.1	Chy2G033440.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010466	negative regulation of peptidase activity
molecular_function	GO:0004869	cysteine-type endopeptidase inhibitor activity