Chy2G031440 (gene) Cucumber (hystrix) v1

Overview
NameChy2G031440
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionCytochrome P450
LocationchrH02: 5651461 .. 5655771 (+)
RNA-Seq ExpressionChy2G031440
SyntenyChy2G031440
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGAATGAAACGGAAAGCCACTTTATGTTAACGGAATGGAGAGCCGATGAAACTGCAGCTTTCTTCAGCCTTTCAGGGCGCCGAATAAGTCGTAGAAAGAGGGAAAACCCACGGTTTGGTGTCGCGTTAACGAGAACCGTCAATGACATTACGCTATGCCGTCGTAGAACTGGGTTAGATGACCCTCCAATCTTATACTGCCATCTGGCTGTACCCTTCATTTACTTTGTCGATCTGACAGCTGGGGGAGCCCGTGATTGCAAAATTAGTTTTTGGTAAAAAATCAATTTTCATGACAAACAATGTGGATAGCTTTCCAATTTTATCAACTTTTAAAGCTGAAACTTGAGATGAGGGTTTGGGACTCATTTTTGCTACTAAATATTATTTAAAATTTCTATTTTAAAATTCTCATTTTACTGTGTTTATTATTTGATTATCAAACAAAATAGTTTACACATCAAACATATATTATCATAATTTAAATTAAAATAATTTATACCTTAGACACAAAATATAATAGTCACCTCTTGTCTCAAATACTGTCGAAGAAAAGTGTCTTTAAAGATAATTTTGAGAGATTAGAAAATCAGTTTATACTAACAAAAAATGTGTTTGATAAAATGAACTGCTCATTACTTTATTTTAGGAGTTATTTTGGAATTGTACTAATATCAAGTTCAAAGTCAATAAAAAAAACCATTTTGTTATTTAGAATGAATTTATAATTTAACTTCAGTTTTAAATTGATTTAATATATTGAAATTTTGTTCTCTTGATCTATTTGGCCTATCAAATAAATAATTCAATTTTAAAATAAAAAATTATAAATTTTTTATTAAAATTCATCATCTCCATGTATATGTATATATAAATATACGCAATGATTTAATTCATCTTAGGTAAATTGTATTAATACAACATCAAATTACCTTTTTATAACTAATGTAAAACCATATTATGACATTCACATTTCTCTCGTATAAATCATAACACATTAAATCGCAACGTTTTGTGAATGTATCCATCACCTCGGTTGAGATGTCATTCATACATTCTCATCGTCGAGTTATGGCTAAGCGAAGTAATCTCAATGCCTCAACAAGTACAACCACTTGCCATACATGGTACTATAATTTCTATATCATATCACAAAGCAATAATATGATATTCTTACACCATATCACACAACAAGTGGAAGTTTTTTCATACTTGATCATAAAGCAAGTATACGTTGTTCGTTACATAGGGTATTAGAACATTTCACAAAACATAAATAATAAGCCATTCACTTTTAAAACATATTCAATTTACAAATTAAATGGCATTATTAAATAAACATCAATAATCAAAGTTCAAATGTGGTTCAAAAATCATTATGGTTTTCAAAACATTTTAAAAACCGTTCACAGTCAATATTAGATTTCCTTATAGCCTAAGCACCTAGTCAAACTCCTTGTCAACCTCAAATGCGTGATAGCTCTGAAAAGTATTTCTCAACCCCTCGTTAATTCTTCAGCTTTTACTTAGCTCGGAGTTCATATTAAGTTTCCAAAACTTACCAGAATCTCCCTTATTTATATACTTTTCTGGTGAGAATTTTCATGATGAAATGATTACTTATGTTTGGTGGTGAGTTTCAACGAATCACATTCGACCACGTAGCTCTCATCGAATGAATTATGATCGTTAGTAAGCGGTTAGATTAGATTGCCAAATGATTGAGCACCGCCTTTCATATGTAAAGAATGAGGCCAACTACTTATAAGTTAATAAATCTTAAGTCAACGCCTCAAATCGAAACACTTCTGGTCAATAAATCTTTATCACACAACTTAGGACGCGATCAGGGTCTCACAATAAACACTAAGGGTGCGTTTGGGAAAAGAAAGAGTTATTAGAAAAAAGGATCATGATAATCCTATGATTATAATTATCATAGGGTTATGATAATATGTGTTTGGGGTTAGGGTTATTATTGGTAGTGTTATGGTAATATGCGTTTGGAAAAAATATTACTATTGTAGTATTATGATAATATGCTTTGGGGGAAGGGTTATACTTGTAATGTTATTATAACTTGTGTTTGGAGGAAGAGTTATGAGTGTAATATTATGATAAGATGAAATAGTGAAATTTGTAGAGTTATGAGAAGATAAAATAGAGAAATTTGTAAGGTTATGAGAAGATAAAATAGAAAAATTTATAGAGTTATTTAACCATAATCCCTAAAAATGCAAACCCTTTTCCTAATTCCCTAAATCCCCACCTCAAACACACTCTAATTGTTTTCAAAATGGACCATCATTTTGAAAAGTAATATGCATTTCTTTCTTTCTGATCGGCAGAATTCCAGTTCGATGTACCACATTTGCTAGTCAACTTTTTGACCACATGCATATGAATTCAAAGGAATGGTAAGGATCTCACAAGAGAAAGTTCAAAGTTGTGGAAGTACAAAAGTTGTTCACTATATGCAACAAATATAAATAAGGAAAGTGCCTTATTATCAAATCATTCACGTAATTACTAATTAATAATATTCATCTCTCCCACAATTTAGAATTTTAAAAATTTTCGAACTTGGAGAATACTCAATTATAATGTCTGTCCTTCTCCATTAGGTATATTAATATATATCAAAACAAATTGAAATCATACCCAAAAAAAAAAAAAAAAAACACCATGGATTTAGACACTTACGTCCTCTATTCTTCTCTCTCACTTTTCTTTCTTCTTCTTGCTTTCAAATTCATCTTTCGATCTCTTCGACTTCGCAATAACTTACCGCCAAGTCCACCCTCTTTTCCGGTCATCGGACATCTCCATCTCTTGAAAAAGCCACTCCACCGAACTTTCAGCAACCTCTCTGCCAAATATGGCCCTGTTATGTCTCTCCGCCTTGGATCCAAGCTTGCAGTCGTTTTATCCTCCTCGGCGGCGGTGGAGGACTGCTTCACTAAAAACGACGTTGTTCTCGCCAACCGTCCACGTTTGCTCATTGGAAAATACATCGGCTATAACTACACTACCATGATTGGGGCTCCATACGGCGACCATTGGCGGAACCTTCGTCGAATTGGCGCCATTGAGATCTTCTCTATATCTCGAATCAACAAATTCACGGACATCCGAAGGGATGAAGTGATGCGAATTGTTCGAAAACTGTCACATAATTCTACCCATCAATCCTCGAAAGTAGAAATTCAATCGGCGATGTCGGAGCTGACGTTCAACATCACGATGAGAATGGCGGCTGGTAAAAGGTATTACGGCGAGGACGTGACGAATGAGGAAGAGGCGAGGAGGTTTAGGGAGTTGATTAAAGAGATTGTGGCGGTAGGAGGAGTATCAAATCCAGGGGATTTCTTACCTGTATTGAATTGGATATCGAAGGGTATTGAGAGGAAATTAATACAGTTGGGGAAGAAAGTGGATGCGTTCTTGCAAGGGCTAATTGATGACCATCGTAAGACGAAGGAAAGGAACACGATGATCGATCACTTGCTTTCTCTACAACAATCGGAACCCGATCACTATAACGATCAAATAATCAAAGGATTTATATTGGTAATTAATAAATTCTCCCACTTCTCTATTTTATTTATCATTTTTTTTTGGAAGAAAGGTTGATTTACACAAATTTGTAGTTAAAAAGTTGTAATAAACTTATTAGAAATATAATAAATTTTGTAGATATTACTAACGGCCGGATCCGACACATCCGCTGTGACAATGGAATGGGCCTTGAGTCATCTCCTAAACAATCCTCAAGTATTAAAAAAGGCAAAAGAGGAGATAGACACTCAAATTGGACAAGAAAGACTCGTGGAGGAATCAGACATTTCTAAACTACCTTATCTTCAAAGAATCATCTCTGAGACTCTTAGGTTGAATCCTGCAGCACCCATGTTGGTTCCCCATTATGCAACCAATGACTGCAAAATATGTGGGTACGATGTGCCGCGAAACACAATGGTACTAGTCAATGCTTGGGCCATACATAGGGATTCAAACGAGTGGGAGGATTGCATGAGTTTCAAACCAGAGCGATACGAAAAGTCAGAAGCTGTGGAGGGACATAAGTTTTTGCCTTTCGGAGTCGGAAGGCGAGCTTGTCCTGGTTCTGCTATGGCACATAGAGTGATGGGTCTTACTTTAGCAGCTCTGATTCAATGTTTTGAGTGGAAGAAAATTGGAGATGAAGACATTGACTTGAAAGAGTTGTCTGGTGCCATAATGACCAAGATGGTGCCATTGGAGGCCATGTGCAAAGCTCGACCAATCATCAACAACATTCTTTACTAA

mRNA sequence

ATGAAGAATGAAACGGAAAGCCACTTTATGTTAACGGAATGGAGAGCCGATGAAACTGCAGCTTTCTTCAGCCTTTCAGGGCGCCGAATAAGTCGTAGAAAGAGGGAAAACCCACGGTTTGGTGTCGCGTTAACGAGAACCGTCAATGACATTACGCTATGCCGTCGTAGAACTGGGTTAGATGACCCTCCAATCTTATACTGCCATCTGGCTCCACTCCACCGAACTTTCAGCAACCTCTCTGCCAAATATGGCCCTGTTATGTCTCTCCGCCTTGGATCCAAGCTTGCAGTCGTTTTATCCTCCTCGGCGGCGGTGGAGGACTGCTTCACTAAAAACGACGTTGTTCTCGCCAACCGTCCACGTTTGCTCATTGGAAAATACATCGGCTATAACTACACTACCATGATTGGGGCTCCATACGGCGACCATTGGCGGAACCTTCGTCGAATTGGCGCCATTGAGATCTTCTCTATATCTCGAATCAACAAATTCACGGACATCCGAAGGGATGAAGTGATGCGAATTGTTCGAAAACTGTCACATAATTCTACCCATCAATCCTCGAAAGTAGAAATTCAATCGGCGATGTCGGAGCTGACGTTCAACATCACGATGAGAATGGCGGCTGGTAAAAGGTATTACGGCGAGGACGTGACGAATGAGGAAGAGGCGAGGAGGTTTAGGGAGTTGATTAAAGAGATTGTGGCGGTAGGAGGAGTATCAAATCCAGGGGATTTCTTACCTGTATTGAATTGGATATCGAAGGGTATTGAGAGGAAATTAATACAGTTGGGGAAGAAAGTGGATGCGTTCTTGCAAGGGCTAATTGATGACCATCGTAAGACGAAGGAAAGGAACACGATGATCGATCACTTGCTTTCTCTACAACAATCGGAACCCGATCACTATAACGATCAAATAATCAAAGGATTTATATTGATATTACTAACGGCCGGATCCGACACATCCGCTGTGACAATGGAATGGGCCTTGAGTCATCTCCTAAACAATCCTCAAGTATTAAAAAAGGCAAAAGAGGAGATAGACACTCAAATTGGACAAGAAAGACTCGTGGAGGAATCAGACATTTCTAAACTACCTTATCTTCAAAGAATCATCTCTGAGACTCTTAGGTTGAATCCTGCAGCACCCATGTTGGTTCCCCATTATGCAACCAATGACTGCAAAATATGTGGGTACGATGTGCCGCGAAACACAATGGTACTAGTCAATGCTTGGGCCATACATAGGGATTCAAACGAGTGGGAGGATTGCATGAGTTTCAAACCAGAGCGATACGAAAAGTCAGAAGCTGTGGAGGGACATAAGTTTTTGCCTTTCGGAGTCGGAAGGCGAGCTTGTCCTGGTTCTGCTATGGCACATAGAGTGATGGGTCTTACTTTAGCAGCTCTGATTCAATGTTTTGAGTGGAAGAAAATTGGAGATGAAGACATTGACTTGAAAGAGTTGTCTGGTGCCATAATGACCAAGATGGTGCCATTGGAGGCCATGTGCAAAGCTCGACCAATCATCAACAACATTCTTTACTAA

Coding sequence (CDS)

ATGAAGAATGAAACGGAAAGCCACTTTATGTTAACGGAATGGAGAGCCGATGAAACTGCAGCTTTCTTCAGCCTTTCAGGGCGCCGAATAAGTCGTAGAAAGAGGGAAAACCCACGGTTTGGTGTCGCGTTAACGAGAACCGTCAATGACATTACGCTATGCCGTCGTAGAACTGGGTTAGATGACCCTCCAATCTTATACTGCCATCTGGCTCCACTCCACCGAACTTTCAGCAACCTCTCTGCCAAATATGGCCCTGTTATGTCTCTCCGCCTTGGATCCAAGCTTGCAGTCGTTTTATCCTCCTCGGCGGCGGTGGAGGACTGCTTCACTAAAAACGACGTTGTTCTCGCCAACCGTCCACGTTTGCTCATTGGAAAATACATCGGCTATAACTACACTACCATGATTGGGGCTCCATACGGCGACCATTGGCGGAACCTTCGTCGAATTGGCGCCATTGAGATCTTCTCTATATCTCGAATCAACAAATTCACGGACATCCGAAGGGATGAAGTGATGCGAATTGTTCGAAAACTGTCACATAATTCTACCCATCAATCCTCGAAAGTAGAAATTCAATCGGCGATGTCGGAGCTGACGTTCAACATCACGATGAGAATGGCGGCTGGTAAAAGGTATTACGGCGAGGACGTGACGAATGAGGAAGAGGCGAGGAGGTTTAGGGAGTTGATTAAAGAGATTGTGGCGGTAGGAGGAGTATCAAATCCAGGGGATTTCTTACCTGTATTGAATTGGATATCGAAGGGTATTGAGAGGAAATTAATACAGTTGGGGAAGAAAGTGGATGCGTTCTTGCAAGGGCTAATTGATGACCATCGTAAGACGAAGGAAAGGAACACGATGATCGATCACTTGCTTTCTCTACAACAATCGGAACCCGATCACTATAACGATCAAATAATCAAAGGATTTATATTGATATTACTAACGGCCGGATCCGACACATCCGCTGTGACAATGGAATGGGCCTTGAGTCATCTCCTAAACAATCCTCAAGTATTAAAAAAGGCAAAAGAGGAGATAGACACTCAAATTGGACAAGAAAGACTCGTGGAGGAATCAGACATTTCTAAACTACCTTATCTTCAAAGAATCATCTCTGAGACTCTTAGGTTGAATCCTGCAGCACCCATGTTGGTTCCCCATTATGCAACCAATGACTGCAAAATATGTGGGTACGATGTGCCGCGAAACACAATGGTACTAGTCAATGCTTGGGCCATACATAGGGATTCAAACGAGTGGGAGGATTGCATGAGTTTCAAACCAGAGCGATACGAAAAGTCAGAAGCTGTGGAGGGACATAAGTTTTTGCCTTTCGGAGTCGGAAGGCGAGCTTGTCCTGGTTCTGCTATGGCACATAGAGTGATGGGTCTTACTTTAGCAGCTCTGATTCAATGTTTTGAGTGGAAGAAAATTGGAGATGAAGACATTGACTTGAAAGAGTTGTCTGGTGCCATAATGACCAAGATGGTGCCATTGGAGGCCATGTGCAAAGCTCGACCAATCATCAACAACATTCTTTACTAA

Protein sequence

MKNETESHFMLTEWRADETAAFFSLSGRRISRRKRENPRFGVALTRTVNDITLCRRRTGLDDPPILYCHLAPLHRTFSNLSAKYGPVMSLRLGSKLAVVLSSSAAVEDCFTKNDVVLANRPRLLIGKYIGYNYTTMIGAPYGDHWRNLRRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSTHQSSKVEIQSAMSELTFNITMRMAAGKRYYGEDVTNEEEARRFRELIKEIVAVGGVSNPGDFLPVLNWISKGIERKLIQLGKKVDAFLQGLIDDHRKTKERNTMIDHLLSLQQSEPDHYNDQIIKGFILILLTAGSDTSAVTMEWALSHLLNNPQVLKKAKEEIDTQIGQERLVEESDISKLPYLQRIISETLRLNPAAPMLVPHYATNDCKICGYDVPRNTMVLVNAWAIHRDSNEWEDCMSFKPERYEKSEAVEGHKFLPFGVGRRACPGSAMAHRVMGLTLAALIQCFEWKKIGDEDIDLKELSGAIMTKMVPLEAMCKARPIINNILY*
Homology
BLAST of Chy2G031440 vs. ExPASy Swiss-Prot
Match: W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)

HSP 1 Score: 545.8 bits (1405), Expect = 5.2e-154
Identity = 266/461 (57.70%), Postives = 350/461 (75.92%), Query Frame = 0

Query: 59  GLDDPPILYCHLAP--LHRTFSNLSAKYGPVMSLRLGSKLAVVLSSSAAVEDCFTKNDVV 118
           GL  P I + HL    LHR+  +LS KYG V SL+LG++L +V+SS AA E+CFTKND+V
Sbjct: 37  GLALPVIGHLHLIGKLLHRSLYDLSKKYGSVFSLQLGNRLVLVVSSPAAAEECFTKNDIV 96

Query: 119 LANRPRLLIGKYIGYNYTTMIGAPYGDHWRNLRRIGAIEIFSISRINKFTDIRRDEVMRI 178
            ANRP  ++GKYIGYNYTTM+G+PYG+HWRNLRR+ A+EIFS   +N+F  IR DEV ++
Sbjct: 97  FANRPLFILGKYIGYNYTTMVGSPYGEHWRNLRRLAAVEIFSAGSLNRFLSIREDEVKQL 156

Query: 179 VRKLSHNSTHQSSKVEIQSAMSELTFNITMRMAAGKRYYGEDVTNEEEARRFRELIKEIV 238
           +  L  +S     KVE++S +SEL+FN+TMRM AGKRY+G+DV + +EA+ FR LI E+ 
Sbjct: 157 LLSLYQSSGQDFGKVEMKSKLSELSFNVTMRMVAGKRYFGQDV-DSDEAKLFRALIGEVF 216

Query: 239 AVGGVSNPGDFLPVLNWIS-KGIERKLIQLGKKVDAFLQGLIDDHRKTKERNTMIDHLLS 298
              G SNPGDF+P L WI  K  E+K+ ++ +++DAFLQ LI + R  K   TMIDHLLS
Sbjct: 217 EHAGASNPGDFVPFLRWIDFKNYEKKVSKISQEMDAFLQRLIHESRINKNNVTMIDHLLS 276

Query: 299 LQQSEPDHYNDQIIKGFILILLTAGSDTSAVTMEWALSHLLNNPQVLKKAKEEIDTQIGQ 358
           LQ+S+P++Y DQIIKG I++LL AG+DTSAVT+EWA+S LLN+P+ L+KA+ EI+TQ+G 
Sbjct: 277 LQESQPEYYTDQIIKGIIMVLLLAGTDTSAVTVEWAMSLLLNHPETLEKARTEIETQVGS 336

Query: 359 ERLVEESDISKLPYLQRIISETLRLNPAAPMLVPHYATNDCKICGYDVPRNTMVLVNAWA 418
            RL+EE D+ KL YL  IISET RL PAAPMLVPH +++DCK+ GYDVP+ T++LVNAWA
Sbjct: 337 NRLIEEQDLPKLTYLHNIISETFRLCPAAPMLVPHESSDDCKVQGYDVPKGTILLVNAWA 396

Query: 419 IHRDSNEWEDCMSFKPERYEKSEAVEGHKFLPFGVGRRACPGSAMAHRVMGLTLAALIQC 478
           IHRD   W++   FKPER+   E +E  K +PFG+GRR+CPGS +A RV+GLTL ALIQC
Sbjct: 397 IHRDPEFWDEPTLFKPERHGGVE-LEPSKLMPFGMGRRSCPGSGLAQRVVGLTLGALIQC 456

Query: 479 FEWKKIGDEDIDLKELSGAIMTKMVPLEAMCKARPIINNIL 517
           FEWK+IG+  ID+ E SG  M K  PLEA+CK R I++ ++
Sbjct: 457 FEWKRIGEAKIDMAEGSGLTMPKAQPLEALCKPRNILHKVV 495

BLAST of Chy2G031440 vs. ExPASy Swiss-Prot
Match: Q6WNQ8 (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)

HSP 1 Score: 527.3 bits (1357), Expect = 1.9e-148
Identity = 255/442 (57.69%), Postives = 332/442 (75.11%), Query Frame = 0

Query: 72  PLHRTFSNLSAKYGPVMSLRLGSKLAVVLSSSAAVEDCFTKNDVVLANRPRLLIGKYIGY 131
           PLH TF  LS KYG + SL  GS+L VV+SS    ++CFTKND+VLANRP  L GKYIGY
Sbjct: 51  PLHHTFHTLSQKYGQIFSLWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGY 110

Query: 132 NYTTMIGAPYGDHWRNLRRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSTHQSSKV 191
           N TT+  +PYGDHWRNLRRI +IEI S  R+N F +IRRDE+MR+++KL+  S +  ++V
Sbjct: 111 NNTTVAQSPYGDHWRNLRRILSIEILSSHRLNSFLEIRRDEIMRLIQKLAQKSYNGFTEV 170

Query: 192 EIQSAMSELTFNITMRMAAGKRYYGE--DVTNEEEARRFRELIKEIVAVGGVSNPGDFLP 251
           E++   SE+TFN  MRM +GKRYYG   DV++ EEAR FR +IKE+V++GG +N GDFL 
Sbjct: 171 ELRPMFSEMTFNTIMRMVSGKRYYGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFLG 230

Query: 252 VLNWIS-KGIERKLIQLGKKVDAFLQGLIDDHRKTKER-NTMIDHLLSLQQSEPDHYNDQ 311
            L W    G+E++L ++ K+ DAFLQGLID+HR  K   NTMIDHLL+ QQS+P++Y DQ
Sbjct: 231 FLRWFDFDGLEKRLKKISKRTDAFLQGLIDEHRFGKRNSNTMIDHLLTQQQSQPEYYTDQ 290

Query: 312 IIKGFILILLTAGSDTSAVTMEWALSHLLNNPQVLKKAKEEIDTQIGQERLVEESDISKL 371
           IIKG ++++L AG+DTS+VT+EWA+S+LLN+P+++KKAK E+DT IG +R V+E DISKL
Sbjct: 291 IIKGLMVVMLLAGTDTSSVTIEWAMSNLLNHPEIMKKAKNELDTHIGHDRQVDEHDISKL 350

Query: 372 PYLQRIISETLRLNPAAPMLVPHYATNDCKICGYDVPRNTMVLVNAWAIHRDSNEWEDCM 431
           PYLQ I+ ETLRL+ AAP+LVPH ++ D  + GY++P+NT+++VNAW IHRD N W D  
Sbjct: 351 PYLQSIVYETLRLHAAAPLLVPHLSSEDFSLGGYNIPQNTILMVNAWVIHRDPNLWSDPT 410

Query: 432 SFKPERYEKSEAVEGHKFLPFGVGRRACPGSAMAHRVMGLTLAALIQCFEWKKIGDEDID 491
            FKPER+EK   V  +K L FG+GRRACPG  ++ R  GLTL  LIQCFEWK+IG+E ID
Sbjct: 411 CFKPERFEKEGEV--NKLLSFGLGRRACPGENLSQRTEGLTLGLLIQCFEWKRIGEEKID 470

Query: 492 LKELSGAIMTKMVPLEAMCKAR 510
           + E  G    K   L AMCK R
Sbjct: 471 MVEAKGITAGKKTSLNAMCKVR 490

BLAST of Chy2G031440 vs. ExPASy Swiss-Prot
Match: P93147 (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2)

HSP 1 Score: 515.4 bits (1326), Expect = 7.5e-145
Identity = 244/448 (54.46%), Postives = 340/448 (75.89%), Query Frame = 0

Query: 72  PLHRTFSNLSAKYGPVMSLRLGSKLAVVLSSSAAVEDCFTKNDVVLANRPRLLIGKYIGY 131
           PLHRTF  LS KYG V SL  GS+L VV+SS++  + CFTKNDVVLANRPR L GKYI Y
Sbjct: 51  PLHRTFKGLSEKYGHVFSLWFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFY 110

Query: 132 NYTTMIGAPYGDHWRNLRRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSTHQSSKV 191
           NYTT+    YG+HWRNLRRI A+++ S  RIN F+ IRRDE  R++ +L+ +S+   +++
Sbjct: 111 NYTTLGSTSYGEHWRNLRRITALDVLSNHRINSFSGIRRDETQRLITRLADDSSTNFAEM 170

Query: 192 EIQSAMSELTFNITMRMAAGKRYYGE--DVTNEEEARRFRELIKEIVAVGGVSNPGDFLP 251
           E+ S + ++TFN  MRM +GKRYYGE  D ++ +EA +FR+++ E++ + G +N  DF+P
Sbjct: 171 ELSSRLYDMTFNNIMRMISGKRYYGEDCDTSDLQEASQFRDMVSELLQLSGANNKTDFMP 230

Query: 252 VLNWIS-KGIERKLIQLGKKVDAFLQGLIDDHRKTKER-NTMIDHLLSLQQSEPDHYNDQ 311
           +L ++  + +E++L  +  K DAFL+GLI++HR  KER NTMIDHLL+LQ S+P++Y DQ
Sbjct: 231 LLRFLDFENLEKRLKDISGKTDAFLRGLIEEHRTKKERANTMIDHLLNLQDSQPEYYTDQ 290

Query: 312 IIKGFILILLTAGSDTSAVTMEWALSHLLNNPQVLKKAKEEIDTQIGQERLVEESDISKL 371
           IIKG  L +L AG+D+SAVT+EW++S+LLN+P+VLKK K+E+DT +GQ+RLV+ESD+ KL
Sbjct: 291 IIKGLALAMLLAGTDSSAVTLEWSMSNLLNHPEVLKKVKDELDTHVGQDRLVDESDLPKL 350

Query: 372 PYLQRIISETLRLNPAAPMLVPHYATNDCKICGYDVPRNTMVLVNAWAIHRDSNEWEDCM 431
            YL+ +I+ETLRL   AP+L+PH  +++C I GY VP++T+VL+NAWAIHRD   W +  
Sbjct: 351 TYLKNVINETLRLYTPAPLLLPHSTSDECNIGGYKVPQDTIVLINAWAIHRDPELWTEAT 410

Query: 432 SFKPERYEKSEAVEGHKFLPFGVGRRACPGSAMAHRVMGLTLAALIQCFEWKKIGDEDID 491
           +FKPER+EK   +E  K + FG+GRRACPG  +A R + +TLA LIQCF+WK I  + ID
Sbjct: 411 TFKPERFEKKGELE--KLIAFGMGRRACPGEGLAIRAISMTLALLIQCFDWKLINGDKID 470

Query: 492 LKELSGAIMTKMVPLEAMCKARPIINNI 516
           L E  G  +TK+VPL+AMCK+RP+IN +
Sbjct: 471 LAERDGFTLTKLVPLKAMCKSRPVINKV 496

BLAST of Chy2G031440 vs. ExPASy Swiss-Prot
Match: Q9FG65 (Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1)

HSP 1 Score: 508.4 bits (1308), Expect = 9.2e-143
Identity = 250/452 (55.31%), Postives = 338/452 (74.78%), Query Frame = 0

Query: 72  PLHRTFSNLSAKY-----GPVMSLRLGSKLAVVLSS-SAAVEDCFTKNDVVLANRPRLLI 131
           P+HRT  + S        G VMSLRLGS+L  V+SS   A E+CF KNDVVLANRP+++I
Sbjct: 53  PIHRTLRSFSETLDHNDGGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVII 112

Query: 132 GKYIGYNYTTMIGAPYGDHWRNLRRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNST 191
           GK++GYN T MI APYGDHWRNLRR+  IEIFS  R+N F  +R DEV R++ +LS  + 
Sbjct: 113 GKHVGYNNTNMIAAPYGDHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRLSRLAG 172

Query: 192 HQSSKVEIQSAMSELTFNITMRMAAGKRYYGEDVTNEEEARRFRELIKEIVAVGGVSNPG 251
            + + VE++  + +LTFN  MRM  GKRYYGE+ T+EEEA+R R+L+ ++ A     N  
Sbjct: 173 TKKTVVELKPMLMDLTFNNIMRMMTGKRYYGEETTDEEEAKRVRKLVADVGANTSSGNAV 232

Query: 252 DFLPVLNWISKGIERKLIQLGKKVDAFLQGLIDDHRKTKER-NTMIDHLLSLQQSEPDHY 311
           D++P+L   S   E ++ +LG++ D FLQGLIDD R  +E   TMIDHLL LQ+S+ ++Y
Sbjct: 233 DYVPILRLFS-SYENRVKKLGEETDKFLQGLIDDKRGQQETGTTMIDHLLVLQKSDIEYY 292

Query: 312 NDQIIKGFILILLTAGSDTSAVTMEWALSHLLNNPQVLKKAKEEIDTQIGQERLVEESDI 371
            DQIIKG ILI++ AG++TSAVT+EWALS+LLN+P V+ KA++EID ++G +RL+EE+D+
Sbjct: 293 TDQIIKGIILIMVIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEADL 352

Query: 372 SKLPYLQRIISETLRLNPAAPMLVPHYATNDCKICGYDVPRNTMVLVNAWAIHRDSNEWE 431
           S+LPYL+ I+ ETLRL+PA P+LVPH A+ DCKI  YD+PR T +LVNAWAIHRD N W+
Sbjct: 353 SELPYLKNIVLETLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWD 412

Query: 432 DCMSFKPERYEKSEAVEGHKFLPFGVGRRACPGSAMAHRVMGLTLAALIQCFEWKKIGDE 491
           D  SFKPER+EK E  E  K L FG+GRRACPGS +A R++GL L +LIQCFEW+++G+ 
Sbjct: 413 DPDSFKPERFEKEE--EAQKLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERVGNV 472

Query: 492 DIDLKELSGAIMTKMVPLEAMCKARPIINNIL 517
           ++D+KE  G  + K +PL+A+CKARP ++ I+
Sbjct: 473 EVDMKEGVGNTVPKAIPLKAICKARPFLHKII 501

BLAST of Chy2G031440 vs. ExPASy Swiss-Prot
Match: Q6WNQ9 (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)

HSP 1 Score: 508.4 bits (1308), Expect = 9.2e-143
Identity = 241/453 (53.20%), Postives = 337/453 (74.39%), Query Frame = 0

Query: 72  PLHRTFSNLSAKYGPVMSLRLGSKLAVVLSSSAAVEDCFTKNDVVLANRPRLLIGKYIGY 131
           PLHRTF+ LS  YG + SL  GS+L VV+SS +   +CFTKND++LANRPR L GKYI Y
Sbjct: 49  PLHRTFTTLSQTYGDIFSLWFGSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFY 108

Query: 132 NYTTMIGAPYGDHWRNLRRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSTHQS--- 191
           NYTT+  A YGDHWRNLRRI  I++ S +R+N F  +RRDE  R+++KL  +   +    
Sbjct: 109 NYTTLGSASYGDHWRNLRRITTIDVLSNNRLNSFLGVRRDETNRLIQKLLKDVVSEGFGF 168

Query: 192 SKVEIQSAMSELTFNITMRMAAGKRYYGE--DVTNEEEARRFRELIKEIVAVGGVSNPGD 251
           +KVE++  ++E+TFN  MRM +GKRYYG+  DV++ EEA++FRE+I E++++ G +N GD
Sbjct: 169 TKVELRPRLTEMTFNAMMRMISGKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGD 228

Query: 252 FLPVLNWIS-KGIERKLIQLGKKVDAFLQGLIDDHRK---TKERNTMIDHLLSLQQSEPD 311
           FLP+L  +    +E++  ++ K+ +AFL+GLI++HR+     +  TMIDHLL L +S+P+
Sbjct: 229 FLPLLRVVDLDNLEKRCKRIAKRSNAFLEGLIEEHRRGNIHSDGGTMIDHLLKLSESQPE 288

Query: 312 HYNDQIIKGFILILLTAGSDTSAVTMEWALSHLLNNPQVLKKAKEEIDTQIGQERLVEES 371
           +Y+D +IKG I  +L AG+DTSAVT+EW +S LLN+P+VLKKAKEE+DTQIG+ +LV+E 
Sbjct: 289 YYSDHLIKGLIQGMLLAGTDTSAVTIEWVMSELLNHPEVLKKAKEELDTQIGKNKLVDEQ 348

Query: 372 DISKLPYLQRIISETLRLNPAAPMLVPHYATNDCKICGYDVPRNTMVLVNAWAIHRDSNE 431
           D+SKLPYLQ IISETLRL+P AP+L+PHY++ DC I  ++VP++T++L N W IHRD   
Sbjct: 349 DLSKLPYLQNIISETLRLHPPAPLLLPHYSSEDCTIGEFNVPKDTIILTNVWGIHRDPKH 408

Query: 432 WEDCMSFKPERYEKSEAVEGHKFLPFGVGRRACPGSAMAHRVMGLTLAALIQCFEWKKIG 491
           W D +SFKPER+EK E V  +K + FG+GRRACPG ++A R +G T+  LIQCFEW++  
Sbjct: 409 WNDALSFKPERFEKEEEV--NKVMAFGLGRRACPGLSLAQRTVGFTVGLLIQCFEWERES 468

Query: 492 DEDIDLKELSGAIMTKMVPLEAMCKARPIINNI 516
           +E +D+ E  G  M   +PL AMCKA PI N++
Sbjct: 469 EEKLDMMEGKGITMPMKIPLRAMCKALPIANDV 499

BLAST of Chy2G031440 vs. ExPASy TrEMBL
Match: A0A0A0KFV2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G492250 PE=3 SV=1)

HSP 1 Score: 874.8 bits (2259), Expect = 1.8e-250
Identity = 437/459 (95.21%), Postives = 446/459 (97.17%), Query Frame = 0

Query: 63  PPILYCHL--APLHRTFSNLSAKYGPVMSLRLGSKLAVVLSSSAAVEDCFTKNDVVLANR 122
           P I + HL   P+HRTFSNLSAKYGPVMSLRLGSKLAVVLSSSAAVEDCFTKNDVVLANR
Sbjct: 42  PVIGHLHLLKKPIHRTFSNLSAKYGPVMSLRLGSKLAVVLSSSAAVEDCFTKNDVVLANR 101

Query: 123 PRLLIGKYIGYNYTTMIGAPYGDHWRNLRRIGAIEIFSISRINKFTDIRRDEVMRIVRKL 182
           PRLL+GKYIGYNYTTMIGAPYGDHWRNLRRIGAIEIFSISRINKFTDIRRDEVMRIVRKL
Sbjct: 102 PRLLVGKYIGYNYTTMIGAPYGDHWRNLRRIGAIEIFSISRINKFTDIRRDEVMRIVRKL 161

Query: 183 SHNSTHQSSKVEIQSAMSELTFNITMRMAAGKRYYGEDVTNEEEARRFRELIKEIVAVGG 242
           SHNS HQ SKVEIQSAMSELTFNITMRMAAGKRYYGEDVTNEEEARRFRELIKEIVAVGG
Sbjct: 162 SHNSIHQFSKVEIQSAMSELTFNITMRMAAGKRYYGEDVTNEEEARRFRELIKEIVAVGG 221

Query: 243 VSNPGDFLPVLNWISKGIERKLIQLGKKVDAFLQGLIDDHRKTKE--RNTMIDHLLSLQQ 302
           VSNPGDFLPVLNW+SKGIERKLIQLGKKVDAFLQGLIDDHRKTKE  RNTMIDHLLSLQQ
Sbjct: 222 VSNPGDFLPVLNWMSKGIERKLIQLGKKVDAFLQGLIDDHRKTKEEGRNTMIDHLLSLQQ 281

Query: 303 SEPDHYNDQIIKGFILILLTAGSDTSAVTMEWALSHLLNNPQVLKKAKEEIDTQIGQERL 362
           SEPDHYNDQIIKGFILILLTAGSDTSAVTMEWALSHLLNNPQVLKKAKEEIDTQIGQERL
Sbjct: 282 SEPDHYNDQIIKGFILILLTAGSDTSAVTMEWALSHLLNNPQVLKKAKEEIDTQIGQERL 341

Query: 363 VEESDISKLPYLQRIISETLRLNPAAPMLVPHYATNDCKICGYDVPRNTMVLVNAWAIHR 422
           VEESDISKLPYLQRI+SETLRLNPAAPMLVPHYATNDCKICGYDVPR+TMVLVNAWAIHR
Sbjct: 342 VEESDISKLPYLQRIVSETLRLNPAAPMLVPHYATNDCKICGYDVPRDTMVLVNAWAIHR 401

Query: 423 DSNEWEDCMSFKPERYEKSEAVEGHKFLPFGVGRRACPGSAMAHRVMGLTLAALIQCFEW 482
           DSNEWEDCMSFKPERYEKSEAVE HKFLPFG+GRRACPGSAMAHRVMGLTLA LIQCFEW
Sbjct: 402 DSNEWEDCMSFKPERYEKSEAVEIHKFLPFGLGRRACPGSAMAHRVMGLTLATLIQCFEW 461

Query: 483 KKIGDEDIDLKELSGAIMTKMVPLEAMCKARPIINNILY 518
           KKIG+EDID+KELSGAIMTKMVPLEAMCKARPIINNI Y
Sbjct: 462 KKIGEEDIDMKELSGAIMTKMVPLEAMCKARPIINNIFY 500

BLAST of Chy2G031440 vs. ExPASy TrEMBL
Match: A0A1S3B0W6 (cytochrome P450 81E8-like OS=Cucumis melo OX=3656 GN=LOC103484812 PE=3 SV=1)

HSP 1 Score: 843.2 bits (2177), Expect = 5.8e-241
Identity = 420/459 (91.50%), Postives = 436/459 (94.99%), Query Frame = 0

Query: 63  PPILYCHL--APLHRTFSNLSAKYGPVMSLRLGSKLAVVLSSSAAVEDCFTKNDVVLANR 122
           P I + HL   P HRTF NLSAKYGPVMSLRLGSKLAVVLSSSAAVE+CFTKNDVVLANR
Sbjct: 42  PVIGHLHLLKKPPHRTFLNLSAKYGPVMSLRLGSKLAVVLSSSAAVEECFTKNDVVLANR 101

Query: 123 PRLLIGKYIGYNYTTMIGAPYGDHWRNLRRIGAIEIFSISRINKFTDIRRDEVMRIVRKL 182
           PRLLIGKYIGYNYTTM+ APYGDHWRNLRRIGAIEIFSISRINKFTDIRRDEVMRIVRKL
Sbjct: 102 PRLLIGKYIGYNYTTMVAAPYGDHWRNLRRIGAIEIFSISRINKFTDIRRDEVMRIVRKL 161

Query: 183 SHNSTHQSSKVEIQSAMSELTFNITMRMAAGKRYYGEDVTNEEEARRFRELIKEIVAVGG 242
           SHNS HQ SKVEIQSAMSELTFNITMRMAAGKRYYGEDVTNEEEARRFR LIKEIVAVGG
Sbjct: 162 SHNSIHQFSKVEIQSAMSELTFNITMRMAAGKRYYGEDVTNEEEARRFRALIKEIVAVGG 221

Query: 243 VSNPGDFLPVLNWISKGIERKLIQLGKKVDAFLQGLIDDHRKTKE--RNTMIDHLLSLQQ 302
           VSNPGDFLPVLNW+SKGIERKLIQLGKKVDAFLQGLIDDHR TKE  RNTMIDHLLSLQQ
Sbjct: 222 VSNPGDFLPVLNWMSKGIERKLIQLGKKVDAFLQGLIDDHRNTKEEGRNTMIDHLLSLQQ 281

Query: 303 SEPDHYNDQIIKGFILILLTAGSDTSAVTMEWALSHLLNNPQVLKKAKEEIDTQIGQERL 362
           SEPDHYNDQIIKGFIL+LLTAGSDTSAVTMEWAL HLLNNPQVLK A++E+DTQIGQERL
Sbjct: 282 SEPDHYNDQIIKGFILVLLTAGSDTSAVTMEWALCHLLNNPQVLKMARDELDTQIGQERL 341

Query: 363 VEESDISKLPYLQRIISETLRLNPAAPMLVPHYATNDCKICGYDVPRNTMVLVNAWAIHR 422
           VEESDI KLPYLQRII ETLRLNPAAPMLVPHYA+NDCKICGYDVPRNTMVL+NAWAIHR
Sbjct: 342 VEESDIPKLPYLQRIIYETLRLNPAAPMLVPHYASNDCKICGYDVPRNTMVLINAWAIHR 401

Query: 423 DSNEWEDCMSFKPERYEKSEAVEGHKFLPFGVGRRACPGSAMAHRVMGLTLAALIQCFEW 482
           DSNEWED MSFKPERYEKS+A+E HKFLPFGVGRRACPGSAMAHRVMGLTLA LIQCFEW
Sbjct: 402 DSNEWEDHMSFKPERYEKSKAMEVHKFLPFGVGRRACPGSAMAHRVMGLTLATLIQCFEW 461

Query: 483 KKIGDEDIDLKELSGAIMTKMVPLEAMCKARPIINNILY 518
           KKIG+EDID++ELSGA+MTKMVPLEAMCK RPIINNILY
Sbjct: 462 KKIGEEDIDMRELSGALMTKMVPLEAMCKTRPIINNILY 500

BLAST of Chy2G031440 vs. ExPASy TrEMBL
Match: A0A5D3CLS1 (Cytochrome P450 81E8-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G004450 PE=3 SV=1)

HSP 1 Score: 840.9 bits (2171), Expect = 2.9e-240
Identity = 419/459 (91.29%), Postives = 436/459 (94.99%), Query Frame = 0

Query: 63  PPILYCHL--APLHRTFSNLSAKYGPVMSLRLGSKLAVVLSSSAAVEDCFTKNDVVLANR 122
           P I + HL   PLHRTF NLSAKYGPVMSLRLGSKLAVVLSSSAAVE+CFTKNDVVLANR
Sbjct: 42  PVIGHLHLLKKPLHRTFLNLSAKYGPVMSLRLGSKLAVVLSSSAAVEECFTKNDVVLANR 101

Query: 123 PRLLIGKYIGYNYTTMIGAPYGDHWRNLRRIGAIEIFSISRINKFTDIRRDEVMRIVRKL 182
           PRLLIGKYIGYNYTTM+ APYGDHWRNLRRIGAIEIFSISRINKFTDIRRDEVMRIVRKL
Sbjct: 102 PRLLIGKYIGYNYTTMVAAPYGDHWRNLRRIGAIEIFSISRINKFTDIRRDEVMRIVRKL 161

Query: 183 SHNSTHQSSKVEIQSAMSELTFNITMRMAAGKRYYGEDVTNEEEARRFRELIKEIVAVGG 242
           SHNS HQ SKVEIQSAMSELTFNITMRMAAGKRYYGEDVTNEEEARRFR LIKEIVAVGG
Sbjct: 162 SHNSIHQFSKVEIQSAMSELTFNITMRMAAGKRYYGEDVTNEEEARRFRALIKEIVAVGG 221

Query: 243 VSNPGDFLPVLNWISKGIERKLIQLGKKVDAFLQGLIDDHRKTKE--RNTMIDHLLSLQQ 302
           VSNPGDFLPVLNW+SKGIERKLIQLGKKVDAFLQGLIDDHR TKE  RNTMIDHLLSLQQ
Sbjct: 222 VSNPGDFLPVLNWMSKGIERKLIQLGKKVDAFLQGLIDDHRNTKEEGRNTMIDHLLSLQQ 281

Query: 303 SEPDHYNDQIIKGFILILLTAGSDTSAVTMEWALSHLLNNPQVLKKAKEEIDTQIGQERL 362
           SEPDHYNDQIIKGFIL+LLTAGSDTSA+TMEWAL HLLNNPQVLKKA++E+DTQIGQE L
Sbjct: 282 SEPDHYNDQIIKGFILVLLTAGSDTSAMTMEWALCHLLNNPQVLKKARDELDTQIGQEIL 341

Query: 363 VEESDISKLPYLQRIISETLRLNPAAPMLVPHYATNDCKICGYDVPRNTMVLVNAWAIHR 422
           VEESDI KLPYLQRII ETLRLNPAAPMLVPHYA+NDCKICGYDVPRNTMVL+NAWAIHR
Sbjct: 342 VEESDIPKLPYLQRIIYETLRLNPAAPMLVPHYASNDCKICGYDVPRNTMVLINAWAIHR 401

Query: 423 DSNEWEDCMSFKPERYEKSEAVEGHKFLPFGVGRRACPGSAMAHRVMGLTLAALIQCFEW 482
           DSNEWED MSFKPERYEKS+A+E HKFLPFGVGRRACPGSAMAHRVMGLTLA LIQCFEW
Sbjct: 402 DSNEWEDHMSFKPERYEKSKAMEVHKFLPFGVGRRACPGSAMAHRVMGLTLATLIQCFEW 461

Query: 483 KKIGDEDIDLKELSGAIMTKMVPLEAMCKARPIINNILY 518
           KKIG+EDID++ELSGA+MTKMVPLEAMCK R IINNILY
Sbjct: 462 KKIGEEDIDMRELSGALMTKMVPLEAMCKTRSIINNILY 500

BLAST of Chy2G031440 vs. ExPASy TrEMBL
Match: A0A6J1IQ18 (cytochrome P450 81E8-like OS=Cucurbita maxima OX=3661 GN=LOC111477798 PE=3 SV=1)

HSP 1 Score: 762.7 bits (1968), Expect = 1.0e-216
Identity = 378/458 (82.53%), Postives = 414/458 (90.39%), Query Frame = 0

Query: 63  PPILYCHL--APLHRTFSNLSAKYGPVMSLRLGSKLAVVLSSSAAVEDCFTKNDVVLANR 122
           P I + HL   PLHR F NLSAKYGPVMSLRLGS LAVV+SSS+AVE+CFTKNDVVLANR
Sbjct: 41  PVIGHLHLLKKPLHRNFQNLSAKYGPVMSLRLGSNLAVVVSSSSAVEECFTKNDVVLANR 100

Query: 123 PRLLIGKYIGYNYTTMIGAPYGDHWRNLRRIGAIEIFSISRINKFTDIRRDEVMRIVRKL 182
           PRLLIGKY+GYNYTTMIGA YGDHWRNLRRIGAIEIFS SRINKF DIRRDEV R+VRKL
Sbjct: 101 PRLLIGKYLGYNYTTMIGASYGDHWRNLRRIGAIEIFSSSRINKFADIRRDEVKRLVRKL 160

Query: 183 SHNSTHQSSKVEIQSAMSELTFNITMRMAAGKRYYGEDVTNEEEARRFRELIKEIVAVGG 242
           S NS HQ SKVE+QSA+SE+TFNI+MRMAAGKRYYGEDVT++EEARRFRELIKEIVA GG
Sbjct: 161 SRNSIHQYSKVEMQSALSEMTFNISMRMAAGKRYYGEDVTDKEEARRFRELIKEIVAAGG 220

Query: 243 VSNPGDFLPVLNWISKGIERKLIQLGKKVDAFLQGLIDDHRKTKE--RNTMIDHLLSLQQ 302
           VSNPGDF P+LNWISKG ERKLI+LGKKVDAFLQGLIDDHR+ KE  RNTMIDHLLSLQ+
Sbjct: 221 VSNPGDFFPILNWISKGFERKLIELGKKVDAFLQGLIDDHRRKKEEGRNTMIDHLLSLQE 280

Query: 303 SEPDHYNDQIIKGFILILLTAGSDTSAVTMEWALSHLLNNPQVLKKAKEEIDTQIGQERL 362
           S+P  Y+DQIIKGFIL+LLTAG+DTSAVT+EWALSHLLNNP+VLKKAK+EIDTQIGQERL
Sbjct: 281 SQPAVYDDQIIKGFILVLLTAGTDTSAVTVEWALSHLLNNPEVLKKAKDEIDTQIGQERL 340

Query: 363 VEESDISKLPYLQRIISETLRLNPAAPMLVPHYATNDCKICGYDVPRNTMVLVNAWAIHR 422
           VEE D+SKLPYLQ IISETLRL PAAPML+PHYA NDC ICGYDVPR+TM+LVNAWAIHR
Sbjct: 341 VEEPDVSKLPYLQGIISETLRLTPAAPMLLPHYAANDCTICGYDVPRDTMLLVNAWAIHR 400

Query: 423 DSNEWEDCMSFKPERYEKSEAVEGHKFLPFGVGRRACPGSAMAHRVMGLTLAALIQCFEW 482
           D NEWED +SFKPERYEKSE VE HK L FGVGRRACPGSAMAHR+MGLTL ALIQCFEW
Sbjct: 401 DPNEWEDPLSFKPERYEKSE-VEVHKLLSFGVGRRACPGSAMAHRIMGLTLGALIQCFEW 460

Query: 483 KKIGDEDIDLKELSGAIMTKMVPLEAMCKARPIINNIL 517
           ++IG ED+D+ E  GA MTKMVPLEAMC+ARP ++  L
Sbjct: 461 ERIGAEDLDMNERVGATMTKMVPLEAMCRARPTVDGCL 497

BLAST of Chy2G031440 vs. ExPASy TrEMBL
Match: A0A6J1GCP2 (cytochrome P450 81E8-like OS=Cucurbita moschata OX=3662 GN=LOC111452969 PE=3 SV=1)

HSP 1 Score: 756.9 bits (1953), Expect = 5.5e-215
Identity = 375/458 (81.88%), Postives = 413/458 (90.17%), Query Frame = 0

Query: 63  PPILYCHL--APLHRTFSNLSAKYGPVMSLRLGSKLAVVLSSSAAVEDCFTKNDVVLANR 122
           P I + HL   PLHR F NLSAKYGPVMSLRLGS LAVV+SSS+AVE+CFTKNDVVLANR
Sbjct: 41  PVIGHLHLLKKPLHRNFQNLSAKYGPVMSLRLGSNLAVVVSSSSAVEECFTKNDVVLANR 100

Query: 123 PRLLIGKYIGYNYTTMIGAPYGDHWRNLRRIGAIEIFSISRINKFTDIRRDEVMRIVRKL 182
           PRLLIGKY+GYNYTTMIGA YGDHWRNLRRIGAIEIFS SRINKF DIRRDEV R+VRKL
Sbjct: 101 PRLLIGKYLGYNYTTMIGASYGDHWRNLRRIGAIEIFSSSRINKFADIRRDEVKRLVRKL 160

Query: 183 SHNSTHQSSKVEIQSAMSELTFNITMRMAAGKRYYGEDVTNEEEARRFRELIKEIVAVGG 242
           S NS HQ SKVE+QSA+SE+TFNI+MRMAAGKRYYGEDVT++EEARRFRELIKEIVA GG
Sbjct: 161 SRNSIHQYSKVEMQSALSEMTFNISMRMAAGKRYYGEDVTDKEEARRFRELIKEIVAAGG 220

Query: 243 VSNPGDFLPVLNWISKGIERKLIQLGKKVDAFLQGLIDDHRKTKE--RNTMIDHLLSLQQ 302
           VSNPGDF P+LNWISKG ERKL +LGKKVDAFLQGLIDDHR+ KE  RNTMIDHLLSLQ+
Sbjct: 221 VSNPGDFFPILNWISKGFERKLFELGKKVDAFLQGLIDDHRRKKEEGRNTMIDHLLSLQE 280

Query: 303 SEPDHYNDQIIKGFILILLTAGSDTSAVTMEWALSHLLNNPQVLKKAKEEIDTQIGQERL 362
           S+P  Y+DQIIKGFIL+LLTAG+DTSAVT+EWAL HLLNNP+VLKKA++EIDTQIGQERL
Sbjct: 281 SQPAVYDDQIIKGFILVLLTAGTDTSAVTVEWALCHLLNNPEVLKKARDEIDTQIGQERL 340

Query: 363 VEESDISKLPYLQRIISETLRLNPAAPMLVPHYATNDCKICGYDVPRNTMVLVNAWAIHR 422
           VEE D+SKLPYLQ IISETLRL PAAPML+PHYA NDC I GYDVPR+TM+LVNAWAIHR
Sbjct: 341 VEEPDVSKLPYLQGIISETLRLTPAAPMLLPHYAANDCTIHGYDVPRDTMLLVNAWAIHR 400

Query: 423 DSNEWEDCMSFKPERYEKSEAVEGHKFLPFGVGRRACPGSAMAHRVMGLTLAALIQCFEW 482
           D NEWED +SFKPERYEKSE VE HK L FG+GRRACPGSAMAHR+MGLTL ALIQCFEW
Sbjct: 401 DPNEWEDPLSFKPERYEKSE-VEVHKLLSFGLGRRACPGSAMAHRIMGLTLGALIQCFEW 460

Query: 483 KKIGDEDIDLKELSGAIMTKMVPLEAMCKARPIINNIL 517
           +KIG ED+D+ E  GA MTKMVPLEAMC+ARP ++N+L
Sbjct: 461 EKIGAEDLDMNERVGATMTKMVPLEAMCRARPTVDNLL 497

BLAST of Chy2G031440 vs. NCBI nr
Match: XP_004141996.2 (cytochrome P450 81E8 [Cucumis sativus] >KGN48565.1 hypothetical protein Csa_003503 [Cucumis sativus])

HSP 1 Score: 874 bits (2258), Expect = 0.0
Identity = 437/459 (95.21%), Postives = 446/459 (97.17%), Query Frame = 0

Query: 63  PPILYCHLA--PLHRTFSNLSAKYGPVMSLRLGSKLAVVLSSSAAVEDCFTKNDVVLANR 122
           P I + HL   P+HRTFSNLSAKYGPVMSLRLGSKLAVVLSSSAAVEDCFTKNDVVLANR
Sbjct: 42  PVIGHLHLLKKPIHRTFSNLSAKYGPVMSLRLGSKLAVVLSSSAAVEDCFTKNDVVLANR 101

Query: 123 PRLLIGKYIGYNYTTMIGAPYGDHWRNLRRIGAIEIFSISRINKFTDIRRDEVMRIVRKL 182
           PRLL+GKYIGYNYTTMIGAPYGDHWRNLRRIGAIEIFSISRINKFTDIRRDEVMRIVRKL
Sbjct: 102 PRLLVGKYIGYNYTTMIGAPYGDHWRNLRRIGAIEIFSISRINKFTDIRRDEVMRIVRKL 161

Query: 183 SHNSTHQSSKVEIQSAMSELTFNITMRMAAGKRYYGEDVTNEEEARRFRELIKEIVAVGG 242
           SHNS HQ SKVEIQSAMSELTFNITMRMAAGKRYYGEDVTNEEEARRFRELIKEIVAVGG
Sbjct: 162 SHNSIHQFSKVEIQSAMSELTFNITMRMAAGKRYYGEDVTNEEEARRFRELIKEIVAVGG 221

Query: 243 VSNPGDFLPVLNWISKGIERKLIQLGKKVDAFLQGLIDDHRKTKE--RNTMIDHLLSLQQ 302
           VSNPGDFLPVLNW+SKGIERKLIQLGKKVDAFLQGLIDDHRKTKE  RNTMIDHLLSLQQ
Sbjct: 222 VSNPGDFLPVLNWMSKGIERKLIQLGKKVDAFLQGLIDDHRKTKEEGRNTMIDHLLSLQQ 281

Query: 303 SEPDHYNDQIIKGFILILLTAGSDTSAVTMEWALSHLLNNPQVLKKAKEEIDTQIGQERL 362
           SEPDHYNDQIIKGFILILLTAGSDTSAVTMEWALSHLLNNPQVLKKAKEEIDTQIGQERL
Sbjct: 282 SEPDHYNDQIIKGFILILLTAGSDTSAVTMEWALSHLLNNPQVLKKAKEEIDTQIGQERL 341

Query: 363 VEESDISKLPYLQRIISETLRLNPAAPMLVPHYATNDCKICGYDVPRNTMVLVNAWAIHR 422
           VEESDISKLPYLQRI+SETLRLNPAAPMLVPHYATNDCKICGYDVPR+TMVLVNAWAIHR
Sbjct: 342 VEESDISKLPYLQRIVSETLRLNPAAPMLVPHYATNDCKICGYDVPRDTMVLVNAWAIHR 401

Query: 423 DSNEWEDCMSFKPERYEKSEAVEGHKFLPFGVGRRACPGSAMAHRVMGLTLAALIQCFEW 482
           DSNEWEDCMSFKPERYEKSEAVE HKFLPFG+GRRACPGSAMAHRVMGLTLA LIQCFEW
Sbjct: 402 DSNEWEDCMSFKPERYEKSEAVEIHKFLPFGLGRRACPGSAMAHRVMGLTLATLIQCFEW 461

Query: 483 KKIGDEDIDLKELSGAIMTKMVPLEAMCKARPIINNILY 517
           KKIG+EDID+KELSGAIMTKMVPLEAMCKARPIINNI Y
Sbjct: 462 KKIGEEDIDMKELSGAIMTKMVPLEAMCKARPIINNIFY 500

BLAST of Chy2G031440 vs. NCBI nr
Match: XP_008440331.1 (PREDICTED: cytochrome P450 81E8-like [Cucumis melo])

HSP 1 Score: 842 bits (2176), Expect = 3.43e-305
Identity = 420/459 (91.50%), Postives = 436/459 (94.99%), Query Frame = 0

Query: 63  PPILYCHLA--PLHRTFSNLSAKYGPVMSLRLGSKLAVVLSSSAAVEDCFTKNDVVLANR 122
           P I + HL   P HRTF NLSAKYGPVMSLRLGSKLAVVLSSSAAVE+CFTKNDVVLANR
Sbjct: 42  PVIGHLHLLKKPPHRTFLNLSAKYGPVMSLRLGSKLAVVLSSSAAVEECFTKNDVVLANR 101

Query: 123 PRLLIGKYIGYNYTTMIGAPYGDHWRNLRRIGAIEIFSISRINKFTDIRRDEVMRIVRKL 182
           PRLLIGKYIGYNYTTM+ APYGDHWRNLRRIGAIEIFSISRINKFTDIRRDEVMRIVRKL
Sbjct: 102 PRLLIGKYIGYNYTTMVAAPYGDHWRNLRRIGAIEIFSISRINKFTDIRRDEVMRIVRKL 161

Query: 183 SHNSTHQSSKVEIQSAMSELTFNITMRMAAGKRYYGEDVTNEEEARRFRELIKEIVAVGG 242
           SHNS HQ SKVEIQSAMSELTFNITMRMAAGKRYYGEDVTNEEEARRFR LIKEIVAVGG
Sbjct: 162 SHNSIHQFSKVEIQSAMSELTFNITMRMAAGKRYYGEDVTNEEEARRFRALIKEIVAVGG 221

Query: 243 VSNPGDFLPVLNWISKGIERKLIQLGKKVDAFLQGLIDDHRKTKE--RNTMIDHLLSLQQ 302
           VSNPGDFLPVLNW+SKGIERKLIQLGKKVDAFLQGLIDDHR TKE  RNTMIDHLLSLQQ
Sbjct: 222 VSNPGDFLPVLNWMSKGIERKLIQLGKKVDAFLQGLIDDHRNTKEEGRNTMIDHLLSLQQ 281

Query: 303 SEPDHYNDQIIKGFILILLTAGSDTSAVTMEWALSHLLNNPQVLKKAKEEIDTQIGQERL 362
           SEPDHYNDQIIKGFIL+LLTAGSDTSAVTMEWAL HLLNNPQVLK A++E+DTQIGQERL
Sbjct: 282 SEPDHYNDQIIKGFILVLLTAGSDTSAVTMEWALCHLLNNPQVLKMARDELDTQIGQERL 341

Query: 363 VEESDISKLPYLQRIISETLRLNPAAPMLVPHYATNDCKICGYDVPRNTMVLVNAWAIHR 422
           VEESDI KLPYLQRII ETLRLNPAAPMLVPHYA+NDCKICGYDVPRNTMVL+NAWAIHR
Sbjct: 342 VEESDIPKLPYLQRIIYETLRLNPAAPMLVPHYASNDCKICGYDVPRNTMVLINAWAIHR 401

Query: 423 DSNEWEDCMSFKPERYEKSEAVEGHKFLPFGVGRRACPGSAMAHRVMGLTLAALIQCFEW 482
           DSNEWED MSFKPERYEKS+A+E HKFLPFGVGRRACPGSAMAHRVMGLTLA LIQCFEW
Sbjct: 402 DSNEWEDHMSFKPERYEKSKAMEVHKFLPFGVGRRACPGSAMAHRVMGLTLATLIQCFEW 461

Query: 483 KKIGDEDIDLKELSGAIMTKMVPLEAMCKARPIINNILY 517
           KKIG+EDID++ELSGA+MTKMVPLEAMCK RPIINNILY
Sbjct: 462 KKIGEEDIDMRELSGALMTKMVPLEAMCKTRPIINNILY 500

BLAST of Chy2G031440 vs. NCBI nr
Match: TYK12853.1 (cytochrome P450 81E8-like [Cucumis melo var. makuwa])

HSP 1 Score: 840 bits (2170), Expect = 2.81e-304
Identity = 419/459 (91.29%), Postives = 436/459 (94.99%), Query Frame = 0

Query: 63  PPILYCHLA--PLHRTFSNLSAKYGPVMSLRLGSKLAVVLSSSAAVEDCFTKNDVVLANR 122
           P I + HL   PLHRTF NLSAKYGPVMSLRLGSKLAVVLSSSAAVE+CFTKNDVVLANR
Sbjct: 42  PVIGHLHLLKKPLHRTFLNLSAKYGPVMSLRLGSKLAVVLSSSAAVEECFTKNDVVLANR 101

Query: 123 PRLLIGKYIGYNYTTMIGAPYGDHWRNLRRIGAIEIFSISRINKFTDIRRDEVMRIVRKL 182
           PRLLIGKYIGYNYTTM+ APYGDHWRNLRRIGAIEIFSISRINKFTDIRRDEVMRIVRKL
Sbjct: 102 PRLLIGKYIGYNYTTMVAAPYGDHWRNLRRIGAIEIFSISRINKFTDIRRDEVMRIVRKL 161

Query: 183 SHNSTHQSSKVEIQSAMSELTFNITMRMAAGKRYYGEDVTNEEEARRFRELIKEIVAVGG 242
           SHNS HQ SKVEIQSAMSELTFNITMRMAAGKRYYGEDVTNEEEARRFR LIKEIVAVGG
Sbjct: 162 SHNSIHQFSKVEIQSAMSELTFNITMRMAAGKRYYGEDVTNEEEARRFRALIKEIVAVGG 221

Query: 243 VSNPGDFLPVLNWISKGIERKLIQLGKKVDAFLQGLIDDHRKTKE--RNTMIDHLLSLQQ 302
           VSNPGDFLPVLNW+SKGIERKLIQLGKKVDAFLQGLIDDHR TKE  RNTMIDHLLSLQQ
Sbjct: 222 VSNPGDFLPVLNWMSKGIERKLIQLGKKVDAFLQGLIDDHRNTKEEGRNTMIDHLLSLQQ 281

Query: 303 SEPDHYNDQIIKGFILILLTAGSDTSAVTMEWALSHLLNNPQVLKKAKEEIDTQIGQERL 362
           SEPDHYNDQIIKGFIL+LLTAGSDTSA+TMEWAL HLLNNPQVLKKA++E+DTQIGQE L
Sbjct: 282 SEPDHYNDQIIKGFILVLLTAGSDTSAMTMEWALCHLLNNPQVLKKARDELDTQIGQEIL 341

Query: 363 VEESDISKLPYLQRIISETLRLNPAAPMLVPHYATNDCKICGYDVPRNTMVLVNAWAIHR 422
           VEESDI KLPYLQRII ETLRLNPAAPMLVPHYA+NDCKICGYDVPRNTMVL+NAWAIHR
Sbjct: 342 VEESDIPKLPYLQRIIYETLRLNPAAPMLVPHYASNDCKICGYDVPRNTMVLINAWAIHR 401

Query: 423 DSNEWEDCMSFKPERYEKSEAVEGHKFLPFGVGRRACPGSAMAHRVMGLTLAALIQCFEW 482
           DSNEWED MSFKPERYEKS+A+E HKFLPFGVGRRACPGSAMAHRVMGLTLA LIQCFEW
Sbjct: 402 DSNEWEDHMSFKPERYEKSKAMEVHKFLPFGVGRRACPGSAMAHRVMGLTLATLIQCFEW 461

Query: 483 KKIGDEDIDLKELSGAIMTKMVPLEAMCKARPIINNILY 517
           KKIG+EDID++ELSGA+MTKMVPLEAMCK R IINNILY
Sbjct: 462 KKIGEEDIDMRELSGALMTKMVPLEAMCKTRSIINNILY 500

BLAST of Chy2G031440 vs. NCBI nr
Match: XP_022977458.1 (cytochrome P450 81E8-like [Cucurbita maxima])

HSP 1 Score: 762 bits (1967), Expect = 2.15e-273
Identity = 378/458 (82.53%), Postives = 414/458 (90.39%), Query Frame = 0

Query: 63  PPILYCHLA--PLHRTFSNLSAKYGPVMSLRLGSKLAVVLSSSAAVEDCFTKNDVVLANR 122
           P I + HL   PLHR F NLSAKYGPVMSLRLGS LAVV+SSS+AVE+CFTKNDVVLANR
Sbjct: 41  PVIGHLHLLKKPLHRNFQNLSAKYGPVMSLRLGSNLAVVVSSSSAVEECFTKNDVVLANR 100

Query: 123 PRLLIGKYIGYNYTTMIGAPYGDHWRNLRRIGAIEIFSISRINKFTDIRRDEVMRIVRKL 182
           PRLLIGKY+GYNYTTMIGA YGDHWRNLRRIGAIEIFS SRINKF DIRRDEV R+VRKL
Sbjct: 101 PRLLIGKYLGYNYTTMIGASYGDHWRNLRRIGAIEIFSSSRINKFADIRRDEVKRLVRKL 160

Query: 183 SHNSTHQSSKVEIQSAMSELTFNITMRMAAGKRYYGEDVTNEEEARRFRELIKEIVAVGG 242
           S NS HQ SKVE+QSA+SE+TFNI+MRMAAGKRYYGEDVT++EEARRFRELIKEIVA GG
Sbjct: 161 SRNSIHQYSKVEMQSALSEMTFNISMRMAAGKRYYGEDVTDKEEARRFRELIKEIVAAGG 220

Query: 243 VSNPGDFLPVLNWISKGIERKLIQLGKKVDAFLQGLIDDHRKTKE--RNTMIDHLLSLQQ 302
           VSNPGDF P+LNWISKG ERKLI+LGKKVDAFLQGLIDDHR+ KE  RNTMIDHLLSLQ+
Sbjct: 221 VSNPGDFFPILNWISKGFERKLIELGKKVDAFLQGLIDDHRRKKEEGRNTMIDHLLSLQE 280

Query: 303 SEPDHYNDQIIKGFILILLTAGSDTSAVTMEWALSHLLNNPQVLKKAKEEIDTQIGQERL 362
           S+P  Y+DQIIKGFIL+LLTAG+DTSAVT+EWALSHLLNNP+VLKKAK+EIDTQIGQERL
Sbjct: 281 SQPAVYDDQIIKGFILVLLTAGTDTSAVTVEWALSHLLNNPEVLKKAKDEIDTQIGQERL 340

Query: 363 VEESDISKLPYLQRIISETLRLNPAAPMLVPHYATNDCKICGYDVPRNTMVLVNAWAIHR 422
           VEE D+SKLPYLQ IISETLRL PAAPML+PHYA NDC ICGYDVPR+TM+LVNAWAIHR
Sbjct: 341 VEEPDVSKLPYLQGIISETLRLTPAAPMLLPHYAANDCTICGYDVPRDTMLLVNAWAIHR 400

Query: 423 DSNEWEDCMSFKPERYEKSEAVEGHKFLPFGVGRRACPGSAMAHRVMGLTLAALIQCFEW 482
           D NEWED +SFKPERYEKSE VE HK L FGVGRRACPGSAMAHR+MGLTL ALIQCFEW
Sbjct: 401 DPNEWEDPLSFKPERYEKSE-VEVHKLLSFGVGRRACPGSAMAHRIMGLTLGALIQCFEW 460

Query: 483 KKIGDEDIDLKELSGAIMTKMVPLEAMCKARPIINNIL 516
           ++IG ED+D+ E  GA MTKMVPLEAMC+ARP ++  L
Sbjct: 461 ERIGAEDLDMNERVGATMTKMVPLEAMCRARPTVDGCL 497

BLAST of Chy2G031440 vs. NCBI nr
Match: XP_022949637.1 (cytochrome P450 81E8-like [Cucurbita moschata])

HSP 1 Score: 756 bits (1952), Expect = 3.97e-271
Identity = 375/458 (81.88%), Postives = 413/458 (90.17%), Query Frame = 0

Query: 63  PPILYCHLA--PLHRTFSNLSAKYGPVMSLRLGSKLAVVLSSSAAVEDCFTKNDVVLANR 122
           P I + HL   PLHR F NLSAKYGPVMSLRLGS LAVV+SSS+AVE+CFTKNDVVLANR
Sbjct: 41  PVIGHLHLLKKPLHRNFQNLSAKYGPVMSLRLGSNLAVVVSSSSAVEECFTKNDVVLANR 100

Query: 123 PRLLIGKYIGYNYTTMIGAPYGDHWRNLRRIGAIEIFSISRINKFTDIRRDEVMRIVRKL 182
           PRLLIGKY+GYNYTTMIGA YGDHWRNLRRIGAIEIFS SRINKF DIRRDEV R+VRKL
Sbjct: 101 PRLLIGKYLGYNYTTMIGASYGDHWRNLRRIGAIEIFSSSRINKFADIRRDEVKRLVRKL 160

Query: 183 SHNSTHQSSKVEIQSAMSELTFNITMRMAAGKRYYGEDVTNEEEARRFRELIKEIVAVGG 242
           S NS HQ SKVE+QSA+SE+TFNI+MRMAAGKRYYGEDVT++EEARRFRELIKEIVA GG
Sbjct: 161 SRNSIHQYSKVEMQSALSEMTFNISMRMAAGKRYYGEDVTDKEEARRFRELIKEIVAAGG 220

Query: 243 VSNPGDFLPVLNWISKGIERKLIQLGKKVDAFLQGLIDDHRKTKE--RNTMIDHLLSLQQ 302
           VSNPGDF P+LNWISKG ERKL +LGKKVDAFLQGLIDDHR+ KE  RNTMIDHLLSLQ+
Sbjct: 221 VSNPGDFFPILNWISKGFERKLFELGKKVDAFLQGLIDDHRRKKEEGRNTMIDHLLSLQE 280

Query: 303 SEPDHYNDQIIKGFILILLTAGSDTSAVTMEWALSHLLNNPQVLKKAKEEIDTQIGQERL 362
           S+P  Y+DQIIKGFIL+LLTAG+DTSAVT+EWAL HLLNNP+VLKKA++EIDTQIGQERL
Sbjct: 281 SQPAVYDDQIIKGFILVLLTAGTDTSAVTVEWALCHLLNNPEVLKKARDEIDTQIGQERL 340

Query: 363 VEESDISKLPYLQRIISETLRLNPAAPMLVPHYATNDCKICGYDVPRNTMVLVNAWAIHR 422
           VEE D+SKLPYLQ IISETLRL PAAPML+PHYA NDC I GYDVPR+TM+LVNAWAIHR
Sbjct: 341 VEEPDVSKLPYLQGIISETLRLTPAAPMLLPHYAANDCTIHGYDVPRDTMLLVNAWAIHR 400

Query: 423 DSNEWEDCMSFKPERYEKSEAVEGHKFLPFGVGRRACPGSAMAHRVMGLTLAALIQCFEW 482
           D NEWED +SFKPERYEKSE VE HK L FG+GRRACPGSAMAHR+MGLTL ALIQCFEW
Sbjct: 401 DPNEWEDPLSFKPERYEKSE-VEVHKLLSFGLGRRACPGSAMAHRIMGLTLGALIQCFEW 460

Query: 483 KKIGDEDIDLKELSGAIMTKMVPLEAMCKARPIINNIL 516
           +KIG ED+D+ E  GA MTKMVPLEAMC+ARP ++N+L
Sbjct: 461 EKIGAEDLDMNERVGATMTKMVPLEAMCRARPTVDNLL 497

BLAST of Chy2G031440 vs. TAIR 10
Match: AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )

HSP 1 Score: 533.9 bits (1374), Expect = 1.5e-151
Identity = 258/447 (57.72%), Postives = 343/447 (76.73%), Query Frame = 0

Query: 72  PLHRTFSNLSAKY--GPVMSLRLGSKLAVVLSSSAAVEDCFTKNDVVLANRPRLLIGKYI 131
           P+HRTF +LS      P+ SLRLG++L  V SS +  E+CFTKNDVVLANRP  ++ K++
Sbjct: 50  PIHRTFLSLSQSLNNAPIFSLRLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHV 109

Query: 132 GYNYTTMIGAPYGDHWRNLRRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSTHQSS 191
            Y+YTTMI A YGDHWRNLRRIG++EIFS  R+N F  IR+DE+ R+V +LS N + +  
Sbjct: 110 AYDYTTMIAASYGDHWRNLRRIGSVEIFSNHRLNSFLSIRKDEIRRLVFRLSRNFSQEFV 169

Query: 192 KVEIQSAMSELTFNITMRMAAGKRYYGEDVTNEEEARRFRELIKEIVAVGGVSNPGDFLP 251
           KV+++S +S+LTFN  +RM AGKRYYG+ V ++ EA+R R+LI ++VA  G  N  D+LP
Sbjct: 170 KVDMKSMLSDLTFNNILRMVAGKRYYGDGVEDDPEAKRVRQLIADVVACAGAGNAVDYLP 229

Query: 252 VLNWISKGIERKLIQLGKKVDAFLQGLIDDHRKTKER-NTMIDHLLSLQQSEPDHYNDQI 311
           VL  +S   E ++ +L  ++D FLQGL+D+ R+ KE+ NTMIDHLL+LQ+S+PD++ D+I
Sbjct: 230 VLRLVS-DYETRVKKLAGRLDEFLQGLVDEKREAKEKGNTMIDHLLTLQESQPDYFTDRI 289

Query: 312 IKGFILILLTAGSDTSAVTMEWALSHLLNNPQVLKKAKEEIDTQIGQERLVEESDISKLP 371
           IKG +L L+ AG+DTSAVT+EWALS++LN+P VL KA++EID +IG +RL++ESDIS LP
Sbjct: 290 IKGNMLALILAGTDTSAVTLEWALSNVLNHPDVLNKARDEIDRKIGLDRLMDESDISNLP 349

Query: 372 YLQRIISETLRLNPAAPMLVPHYATNDCKICGYDVPRNTMVLVNAWAIHRDSNEWEDCMS 431
           YLQ I+SETLRL PAAPML+PH A+ DCK+ GYD+PR T++L N WAIHRD   W+D MS
Sbjct: 350 YLQNIVSETLRLYPAAPMLLPHVASEDCKVAGYDMPRGTILLTNVWAIHRDPQLWDDPMS 409

Query: 432 FKPERYEKSEAVEGHKFLPFGVGRRACPGSAMAHRVMGLTLAALIQCFEWKKIGDEDIDL 491
           FKPER+EK    E  K +PFG+GRRACPGS +AHR++ LTL +LIQC EW+KIG E++D+
Sbjct: 410 FKPERFEKEG--EAQKLMPFGLGRRACPGSGLAHRLINLTLGSLIQCLEWEKIG-EEVDM 469

Query: 492 KELSGAIMTKMVPLEAMCKARPIINNI 516
            E  G  M K  PLEAMC+ARP +  I
Sbjct: 470 SEGKGVTMPKAKPLEAMCRARPSVVKI 492

BLAST of Chy2G031440 vs. TAIR 10
Match: AT4G37340.1 (cytochrome P450, family 81, subfamily D, polypeptide 3 )

HSP 1 Score: 520.0 bits (1338), Expect = 2.2e-147
Identity = 253/450 (56.22%), Postives = 337/450 (74.89%), Query Frame = 0

Query: 72  PLHRTFSNLSAKYG--PVMSLRLGSKLAVVLSSSAAVEDCFTKNDVVLANRPRLLIGKYI 131
           PLHR F ++S   G  P++SLRLG++L  V+SS +  E+CFTKNDVVLANR   L  K+I
Sbjct: 50  PLHRVFLSVSESLGDAPIISLRLGNRLVFVVSSHSLAEECFTKNDVVLANRFNSLASKHI 109

Query: 132 GYNYTTMIGAPYGDHWRNLRRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSTHQSS 191
            Y  TT++ A YGDHWRNLRRIGA+EIFS  R+N F+ IRRDE+ R++  LS NS+ + +
Sbjct: 110 SYGCTTVVTASYGDHWRNLRRIGAVEIFSAHRLNSFSSIRRDEIHRLIACLSRNSSLEFT 169

Query: 192 KVEIQSAMSELTFNITMRMAAGKRYYGEDVTNEEEARRFRELIKEIVAVGGVSNPGDFLP 251
           KVE++S  S LTFN  +RM AGK YYG+   ++ EA+R RELI E +   G  N  D+LP
Sbjct: 170 KVEMKSMFSNLTFNNIIRMLAGKCYYGDGAEDDPEAKRVRELIAEGMGCFGAGNTADYLP 229

Query: 252 VLNWISKGIERKLIQLGKKVDAFLQGLIDDHRKTKER--NTMIDHLLSLQQSEPDHYNDQ 311
           +L WI+ G E+++ ++  ++D FLQGL+D+ R+ KE+  NTM+DHLL LQ+++P++Y D 
Sbjct: 230 ILTWIT-GSEKRIKKIASRLDEFLQGLVDERREGKEKRQNTMVDHLLCLQETQPEYYTDN 289

Query: 312 IIKGFILILLTAGSDTSAVTMEWALSHLLNNPQVLKKAKEEIDTQIGQERLVEESDISKL 371
           IIKG +L L+ AG+DTSAVT+EW LS LLN+PQ+L KA++EID ++G  RLVEESD+S L
Sbjct: 290 IIKGIMLSLILAGTDTSAVTLEWTLSALLNHPQILSKARDEIDNKVGLNRLVEESDLSHL 349

Query: 372 PYLQRIISETLRLNPAAPMLVPHYATNDCKICGYDVPRNTMVLVNAWAIHRDSNEWEDCM 431
           PYLQ I+SE+LRL PA+P+LVPH A+ DCK+ GY +PR TM+L NAWAIHRD   W+D  
Sbjct: 350 PYLQNIVSESLRLYPASPLLVPHVASEDCKVGGYHMPRGTMLLTNAWAIHRDPKIWDDPT 409

Query: 432 SFKPERYEKSEAVEGHKFLPFGVGRRACPGSAMAHRVMGLTLAALIQCFEWKKIGDEDID 491
           SFKPER+EK    E  K L FG+GRRACPGS +A R+  LT+ +LIQCFEW++IG+E++D
Sbjct: 410 SFKPERFEKEG--EAQKLLGFGLGRRACPGSGLAQRLASLTIGSLIQCFEWERIGEEEVD 469

Query: 492 LKE-LSGAIMTKMVPLEAMCKARPIINNIL 517
           + E   G IM K +PL AMCKARP++  IL
Sbjct: 470 MTEGGGGVIMPKAIPLVAMCKARPVVGKIL 496

BLAST of Chy2G031440 vs. TAIR 10
Match: AT4G37360.1 (cytochrome P450, family 81, subfamily D, polypeptide 2 )

HSP 1 Score: 509.2 bits (1310), Expect = 3.8e-144
Identity = 246/449 (54.79%), Postives = 335/449 (74.61%), Query Frame = 0

Query: 72  PLHRTFSNLSAKYG--PVMSLRLGSKLAVVLSSSAAVEDCFTKNDVVLANRPRLLIGKYI 131
           PLHR F ++S   G  P++SLRLG++L  V+SS +  E+CFTKNDV+LANR   +  K+I
Sbjct: 50  PLHRVFLSVSQSLGDAPIISLRLGNRLLFVVSSHSIAEECFTKNDVILANRQTTISTKHI 109

Query: 132 GYNYTTMIGAPYGDHWRNLRRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSTHQSS 191
            Y  +T++ A Y +HWRNLRRIGA+EIFS  R+N F+ IRRDE+ R++ +L  NS++  +
Sbjct: 110 SYGNSTVVSASYSEHWRNLRRIGALEIFSAHRLNSFSSIRRDEIRRLIGRLLRNSSYGFT 169

Query: 192 KVEIQSAMSELTFNITMRMAAGKRYYGEDVTNEEEARRFRELIKEIVAVGGVSNPGDFLP 251
           KVE++S  S+LTFN  +RM AGK YYG+   ++ EA+R R LI E ++  G  N  D++P
Sbjct: 170 KVEMKSMFSDLTFNNIIRMLAGKCYYGDGKEDDPEAKRVRTLIAEAMSSSGPGNAADYIP 229

Query: 252 VLNWISKGIERKLIQLGKKVDAFLQGLIDDHRKTKER--NTMIDHLLSLQQSEPDHYNDQ 311
           +L WI+   E ++ +L  ++D FLQGL+D+ R+ KE+  NTM+DHLL LQ+++P++Y D+
Sbjct: 230 ILTWITYS-ETRIKKLAGRLDEFLQGLVDEKREGKEKKENTMVDHLLCLQETQPEYYMDR 289

Query: 312 IIKGFILILLTAGSDTSAVTMEWALSHLLNNPQVLKKAKEEIDTQIGQERLVEESDISKL 371
           IIKG +L L+  G+DT+AVT+EWALS LLNNP+VL KA++EID  IG +RL+EESDI  L
Sbjct: 290 IIKGTMLSLIAGGTDTTAVTLEWALSSLLNNPEVLNKARDEIDRMIGVDRLLEESDIPNL 349

Query: 372 PYLQRIISETLRLNPAAPMLVPHYATNDCKICGYDVPRNTMVLVNAWAIHRDSNEWEDCM 431
           PYLQ I+SETLRL PAAPML+PH A+ DCK+ GYD+PR TM+L NAWAIHRD   W+D  
Sbjct: 350 PYLQNIVSETLRLYPAAPMLLPHVASKDCKVGGYDMPRGTMLLTNAWAIHRDPLLWDDPT 409

Query: 432 SFKPERYEKSEAVEGHKFLPFGVGRRACPGSAMAHRVMGLTLAALIQCFEWKKIGDEDID 491
           SFKPER+EK    E  K +PFG+GRRACPGS +A R++ L+L +LIQCFEW++IG+E++D
Sbjct: 410 SFKPERFEKEG--EAKKLMPFGLGRRACPGSGLAQRLVTLSLGSLIQCFEWERIGEEEVD 469

Query: 492 LKELSGAIMTKMVPLEAMCKARPIINNIL 517
           + E  G  M K  PLEAMC+AR  +  IL
Sbjct: 470 MTEGPGLTMPKARPLEAMCRARDFVGKIL 495

BLAST of Chy2G031440 vs. TAIR 10
Match: AT3G28740.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 508.4 bits (1308), Expect = 6.6e-144
Identity = 253/460 (55.00%), Postives = 333/460 (72.39%), Query Frame = 0

Query: 63  PPILYCHL--APLHRTFSNLSAKY--GPVMSLRLGSKLAVVLSSSAAVEDCFTKNDVVLA 122
           P I + HL   PLHR F +LS       + SL LGS+L  V+SS A  E+CFTKNDVVLA
Sbjct: 48  PIIGHLHLLKLPLHRRFLSLSESLNNAKIFSLSLGSRLVFVVSSHAVAEECFTKNDVVLA 107

Query: 123 NRPRLLIGKYIGYNYTTMIGAPYGDHWRNLRRIGAIEIFSISRINKFTDIRRDEVMRIVR 182
           NRP  L+GK+IGYN TTM+GA YGD WRNLRRIG IEIFS  R+N F  IR+DE+ R++ 
Sbjct: 108 NRPEFLVGKHIGYNSTTMVGAAYGDSWRNLRRIGTIEIFSSLRLNSFVSIRQDEIRRLII 167

Query: 183 KLSHNSTHQSSKVEIQSAMSELTFNITMRMAAGKRYYGEDVTNEEEARRFRELIKEIVAV 242
            L+ NS H   KVE++     LT N  +RM AGKR+YG+   N+ EA+  R+LI E+V  
Sbjct: 168 CLAKNSQHGFVKVEMKPLFMCLTINNIIRMVAGKRFYGDGTENDNEAKHVRQLIAEVVVS 227

Query: 243 GGVSNPGDFLPVLNWISKGIERKLIQLGKKVDAFLQGLIDDHRKTKER-NTMIDHLLSLQ 302
           GG  N  D+ P+L +++   E+ + +L  +VD FLQ L+++ R  K + NTMIDHLLSLQ
Sbjct: 228 GGAGNAADYFPILRYVT-NYEKHVKKLAGRVDEFLQSLVNEKRVEKVKGNTMIDHLLSLQ 287

Query: 303 QSEPDHYNDQIIKGFILILLTAGSDTSAVTMEWALSHLLNNPQVLKKAKEEIDTQIGQER 362
           +++PD+Y D IIKG IL+++ AG+DTSA T+EWA+S+LLN+P+VL+KAK EID QIG +R
Sbjct: 288 ETQPDYYTDVIIKGIILVMILAGTDTSAGTLEWAMSNLLNHPEVLRKAKTEIDDQIGVDR 347

Query: 363 LVEESDISKLPYLQRIISETLRLNPAAPMLVPHYATNDCKICGYDVPRNTMVLVNAWAIH 422
           LVEE DI KLPYLQ I+SETLRL P APML+PH A+ DC + GYDVPR T++LVNAWAIH
Sbjct: 348 LVEEQDIVKLPYLQHIVSETLRLYPVAPMLLPHLASEDCIVDGYDVPRGTIILVNAWAIH 407

Query: 423 RDSNEWEDCMSFKPERYEKSEAVEGHKFLPFGVGRRACPGSAMAHRVMGLTLAALIQCFE 482
           RD   WE+   FKPER+EK    E  K +PFG+GRR+CPGS +A R++ L L +L+QCFE
Sbjct: 408 RDPKLWEEPEKFKPERFEKKG--EDKKLMPFGIGRRSCPGSGLAQRLVTLALGSLVQCFE 467

Query: 483 WKKIGDEDIDLKEL-SGAIMTKMVPLEAMCKARPIINNIL 517
           W+++ ++ +D++E   G  M K   L+AMCKARPI++ +L
Sbjct: 468 WERVEEKYLDMRESEKGTTMRKATSLQAMCKARPIVHKVL 504

BLAST of Chy2G031440 vs. TAIR 10
Match: AT5G36220.1 (cytochrome p450 81d1 )

HSP 1 Score: 508.4 bits (1308), Expect = 6.6e-144
Identity = 250/452 (55.31%), Postives = 338/452 (74.78%), Query Frame = 0

Query: 72  PLHRTFSNLSAKY-----GPVMSLRLGSKLAVVLSS-SAAVEDCFTKNDVVLANRPRLLI 131
           P+HRT  + S        G VMSLRLGS+L  V+SS   A E+CF KNDVVLANRP+++I
Sbjct: 53  PIHRTLRSFSETLDHNDGGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVII 112

Query: 132 GKYIGYNYTTMIGAPYGDHWRNLRRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNST 191
           GK++GYN T MI APYGDHWRNLRR+  IEIFS  R+N F  +R DEV R++ +LS  + 
Sbjct: 113 GKHVGYNNTNMIAAPYGDHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRLSRLAG 172

Query: 192 HQSSKVEIQSAMSELTFNITMRMAAGKRYYGEDVTNEEEARRFRELIKEIVAVGGVSNPG 251
            + + VE++  + +LTFN  MRM  GKRYYGE+ T+EEEA+R R+L+ ++ A     N  
Sbjct: 173 TKKTVVELKPMLMDLTFNNIMRMMTGKRYYGEETTDEEEAKRVRKLVADVGANTSSGNAV 232

Query: 252 DFLPVLNWISKGIERKLIQLGKKVDAFLQGLIDDHRKTKER-NTMIDHLLSLQQSEPDHY 311
           D++P+L   S   E ++ +LG++ D FLQGLIDD R  +E   TMIDHLL LQ+S+ ++Y
Sbjct: 233 DYVPILRLFS-SYENRVKKLGEETDKFLQGLIDDKRGQQETGTTMIDHLLVLQKSDIEYY 292

Query: 312 NDQIIKGFILILLTAGSDTSAVTMEWALSHLLNNPQVLKKAKEEIDTQIGQERLVEESDI 371
            DQIIKG ILI++ AG++TSAVT+EWALS+LLN+P V+ KA++EID ++G +RL+EE+D+
Sbjct: 293 TDQIIKGIILIMVIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEADL 352

Query: 372 SKLPYLQRIISETLRLNPAAPMLVPHYATNDCKICGYDVPRNTMVLVNAWAIHRDSNEWE 431
           S+LPYL+ I+ ETLRL+PA P+LVPH A+ DCKI  YD+PR T +LVNAWAIHRD N W+
Sbjct: 353 SELPYLKNIVLETLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWD 412

Query: 432 DCMSFKPERYEKSEAVEGHKFLPFGVGRRACPGSAMAHRVMGLTLAALIQCFEWKKIGDE 491
           D  SFKPER+EK E  E  K L FG+GRRACPGS +A R++GL L +LIQCFEW+++G+ 
Sbjct: 413 DPDSFKPERFEKEE--EAQKLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERVGNV 472

Query: 492 DIDLKELSGAIMTKMVPLEAMCKARPIINNIL 517
           ++D+KE  G  + K +PL+A+CKARP ++ I+
Sbjct: 473 EVDMKEGVGNTVPKAIPLKAICKARPFLHKII 501

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
W8JMU75.2e-15457.70Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1[more]
Q6WNQ81.9e-14857.69Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1[more]
P931477.5e-14554.46Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2[more]
Q9FG659.2e-14355.31Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1[more]
Q6WNQ99.2e-14353.20Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 P... [more]
Match NameE-valueIdentityDescription
A0A0A0KFV21.8e-25095.21Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G492250 PE=3 SV=1[more]
A0A1S3B0W65.8e-24191.50cytochrome P450 81E8-like OS=Cucumis melo OX=3656 GN=LOC103484812 PE=3 SV=1[more]
A0A5D3CLS12.9e-24091.29Cytochrome P450 81E8-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
A0A6J1IQ181.0e-21682.53cytochrome P450 81E8-like OS=Cucurbita maxima OX=3661 GN=LOC111477798 PE=3 SV=1[more]
A0A6J1GCP25.5e-21581.88cytochrome P450 81E8-like OS=Cucurbita moschata OX=3662 GN=LOC111452969 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
XP_004141996.20.095.21cytochrome P450 81E8 [Cucumis sativus] >KGN48565.1 hypothetical protein Csa_0035... [more]
XP_008440331.13.43e-30591.50PREDICTED: cytochrome P450 81E8-like [Cucumis melo][more]
TYK12853.12.81e-30491.29cytochrome P450 81E8-like [Cucumis melo var. makuwa][more]
XP_022977458.12.15e-27382.53cytochrome P450 81E8-like [Cucurbita maxima][more]
XP_022949637.13.97e-27181.88cytochrome P450 81E8-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT4G37370.11.5e-15157.72cytochrome P450, family 81, subfamily D, polypeptide 8 [more]
AT4G37340.12.2e-14756.22cytochrome P450, family 81, subfamily D, polypeptide 3 [more]
AT4G37360.13.8e-14454.79cytochrome P450, family 81, subfamily D, polypeptide 2 [more]
AT3G28740.16.6e-14455.00Cytochrome P450 superfamily protein [more]
AT5G36220.16.6e-14455.31cytochrome p450 81d1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 81..100
score: 43.8
coord: 308..325
score: 28.51
coord: 105..126
score: 19.12
coord: 412..436
score: 29.44
coord: 455..478
score: 31.96
coord: 200..218
score: 24.86
coord: 328..354
score: 37.82
coord: 371..389
score: 34.32
coord: 445..455
score: 59.32
IPR001128Cytochrome P450PRINTSPR00385P450coord: 455..466
score: 28.84
coord: 319..336
score: 36.18
coord: 372..383
score: 38.9
coord: 446..455
score: 60.25
IPR001128Cytochrome P450PFAMPF00067p450coord: 72..497
e-value: 1.9E-90
score: 303.9
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 64..508
e-value: 9.1E-116
score: 389.2
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 66..509
NoneNo IPR availablePANTHERPTHR24298FLAVONOID 3'-MONOOXYGENASE-RELATEDcoord: 67..513
NoneNo IPR availablePANTHERPTHR24298:SF836CYTOCHROME P450, FAMILY 81, SUBFAMILY D, POLYPEPTIDE 2-RELATEDcoord: 67..513
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 448..457

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy2G031440.1Chy2G031440.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016709 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen