Chy2G031400 (gene) Cucumber (hystrix) v1

Overview
NameChy2G031400
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionAuxin-responsive GH3 family protein
LocationchrH02: 5624574 .. 5626561 (-)
RNA-Seq ExpressionChy2G031400
SyntenyChy2G031400
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGTTAGTTCTTCGGTTTCCCATGCCAAGGCTCTCCAATTCATTGAGGAGATTACTACAAATGCTGACTCGGTTCAACAGAAGGTGTTGGCTGAGATTTTGAATCGGAATTCTGATACGGAGTATTTGAAACGGTTTCGACTGAGTAGAACCGCCAGTCGAGATGAATTTAAGTCAAATGTTCGAGTGGTTACTTACGAAGATCTTCAACTCGATATCCAACGTATTGCTAATGGCGATCGCTCCCCTATTTTTTCCTCTCATCCCATTTCTGAGTTTCTTACCAGGTGAATATTTAAGAAAATACACTCAATTATTGCATAAATTTTATATTTCCAAGTTTTTTTTTTTTTTTTTTTTTTCTCAATTGCGTTCAATCTGATTTTTAAAATTTTCAGTTCTGGGACATCCGCTGGGGAGAGGAAACTAATGCCGACAATTAAAGAAGAAATGGAACGTCGTCAGCTACTTTACAGCCTTCTCATGCCCATCATGAACAAGTACGACTTTAATTCAATTCAATTCTTGAAGAATCATATATATTAGGATTAGCTCTTCATTTTGATTTTTTTTTTAAAAAAAAATTAACAAATAAATAATAAATTTTCAGGTATGTGCCGGGGTTGGACAAAGGGAAAGGACTTTACTTTTTATTCGTGAAGGCAGAAACAAAGACCCCTGGAGGACTACTGGCACGTCCGGTACTCACTAGCTACTATAAGAGTGACATCTTCAAGACCAGACCATATGATCCTTTCAATGACTACTCTAGCCCAAACGAGGCCATTCTATGCGCCAATTCCTTCCAAAGCATGTACACTCAGATGTTATGTGGTTTATTGATGCGTGAGCAAGTCCTCCGTGTTGGCGCCGTCTTCGCCTCCGGTCTCCTCCGTGCCATTCACTTCCTCCAGCACAACTGGAAACAGCTTGCACACGACATCTCCACCGCAACCTTAAATCCCAAAATAACGGACTCTTGTCTCCGTGAATGTGTAGTCTCCAAATATTTAACAAACCCGAATCCGGAGCTGGCGGAATTCATCTCAAAGGAATGCTCGACGGAGGAGTGGGAAGGGATTATCACGAGAATATGGCCGAATACTAAATATTTGGATGTGATTGTGACTGGAGCCATGGCTCAGTATATTCCCACTCTCGAATTTTACAGCGGTGGTTTACCCATGGCTTGCACTATGTATGCTTCTTCCGAGTGTTATTTTGGGGTAAATTTAAACCCAATGTGTAAACCCTCGGATGTGACTTACACCATCATGCCAAACATGTGCTACTGCGAATTCATCCCCCTCGATAACGATATGTCGTCTCCTCCAACTCAACTGGTTGACCTCGCTGACGTGGAAGTAGGGAAAGAGTACGAGCTCGTGATCACAACATACTCTGGACTCTGTCGTTACCGAGTCGGTGACATCCTACACGTCACTGGGTTCCACAACGCAGCCCCACAGTTTCGATTCGTGAGGAGGAAGAATGTTCTTTTGAGCATTGATTCCGACAAGACGGATGAAGCCGAGTTACAAAAGGCCATAGACAACGCCGCGCTGTTGTTGCGGGGGTTTAACACTAGCGTGGTGGAGTATACGAGCCATGCGGATACGAAAACAATCCCGGGACATTACGTCATTTATTGGGAGCTTCTTGTGAAAGACGAAGAAACTGGAAATTTCCCTCCAGGAGAGATTTTAAATCAGTGTTGTTTGGCTATTGAAGAGAGTTTGAACTCGGTTTACCGACAAGGCCGAGTTGCGGATAACTCAATTGGACCGTTGGAGATTAGGGTGGTGAGAAATGGGACATTTGAAGAGCTAATGGACTATGCGATCTCGAGGGGTGCGTCGATCAACCAGTACAAGGCGCCACGGTGCGTAAACTTCACGCCCATTATCGAGCTTCTCGACTCTAGGGTGACTTCTGTTCACTTTAGTCCATCCAAGCCACACTGGACACCAGAACGACGCGGCTAG

mRNA sequence

ATGGCTGTTAGTTCTTCGGTTTCCCATGCCAAGGCTCTCCAATTCATTGAGGAGATTACTACAAATGCTGACTCGGTTCAACAGAAGGTGTTGGCTGAGATTTTGAATCGGAATTCTGATACGGAGTATTTGAAACGGTTTCGACTGAGTAGAACCGCCAGTCGAGATGAATTTAAGTCAAATGTTCGAGTGGTTACTTACGAAGATCTTCAACTCGATATCCAACGTATTGCTAATGGCGATCGCTCCCCTATTTTTTCCTCTCATCCCATTTCTGAGTTTCTTACCAGTTCTGGGACATCCGCTGGGGAGAGGAAACTAATGCCGACAATTAAAGAAGAAATGGAACGTCGTCAGCTACTTTACAGCCTTCTCATGCCCATCATGAACAAGTATGTGCCGGGGTTGGACAAAGGGAAAGGACTTTACTTTTTATTCGTGAAGGCAGAAACAAAGACCCCTGGAGGACTACTGGCACGTCCGGTACTCACTAGCTACTATAAGAGTGACATCTTCAAGACCAGACCATATGATCCTTTCAATGACTACTCTAGCCCAAACGAGGCCATTCTATGCGCCAATTCCTTCCAAAGCATGTACACTCAGATGTTATGTGGTTTATTGATGCGTGAGCAAGTCCTCCGTGTTGGCGCCGTCTTCGCCTCCGGTCTCCTCCGTGCCATTCACTTCCTCCAGCACAACTGGAAACAGCTTGCACACGACATCTCCACCGCAACCTTAAATCCCAAAATAACGGACTCTTGTCTCCGTGAATGTGTAGTCTCCAAATATTTAACAAACCCGAATCCGGAGCTGGCGGAATTCATCTCAAAGGAATGCTCGACGGAGGAGTGGGAAGGGATTATCACGAGAATATGGCCGAATACTAAATATTTGGATGTGATTGTGACTGGAGCCATGGCTCAGTATATTCCCACTCTCGAATTTTACAGCGGTGGTTTACCCATGGCTTGCACTATGTATGCTTCTTCCGAGTGTTATTTTGGGGTAAATTTAAACCCAATGTGTAAACCCTCGGATGTGACTTACACCATCATGCCAAACATGTGCTACTGCGAATTCATCCCCCTCGATAACGATATGTCGTCTCCTCCAACTCAACTGGTTGACCTCGCTGACGTGGAAGTAGGGAAAGAGTACGAGCTCGTGATCACAACATACTCTGGACTCTGTCGTTACCGAGTCGGTGACATCCTACACGTCACTGGGTTCCACAACGCAGCCCCACAGTTTCGATTCGTGAGGAGGAAGAATGTTCTTTTGAGCATTGATTCCGACAAGACGGATGAAGCCGAGTTACAAAAGGCCATAGACAACGCCGCGCTGTTGTTGCGGGGGTTTAACACTAGCGTGGTGGAGTATACGAGCCATGCGGATACGAAAACAATCCCGGGACATTACGTCATTTATTGGGAGCTTCTTGTGAAAGACGAAGAAACTGGAAATTTCCCTCCAGGAGAGATTTTAAATCAGTGTTGTTTGGCTATTGAAGAGAGTTTGAACTCGGTTTACCGACAAGGCCGAGTTGCGGATAACTCAATTGGACCGTTGGAGATTAGGGTGGTGAGAAATGGGACATTTGAAGAGCTAATGGACTATGCGATCTCGAGGGGTGCGTCGATCAACCAGTACAAGGCGCCACGGTGCGTAAACTTCACGCCCATTATCGAGCTTCTCGACTCTAGGGTGACTTCTGTTCACTTTAGTCCATCCAAGCCACACTGGACACCAGAACGACGCGGCTAG

Coding sequence (CDS)

ATGGCTGTTAGTTCTTCGGTTTCCCATGCCAAGGCTCTCCAATTCATTGAGGAGATTACTACAAATGCTGACTCGGTTCAACAGAAGGTGTTGGCTGAGATTTTGAATCGGAATTCTGATACGGAGTATTTGAAACGGTTTCGACTGAGTAGAACCGCCAGTCGAGATGAATTTAAGTCAAATGTTCGAGTGGTTACTTACGAAGATCTTCAACTCGATATCCAACGTATTGCTAATGGCGATCGCTCCCCTATTTTTTCCTCTCATCCCATTTCTGAGTTTCTTACCAGTTCTGGGACATCCGCTGGGGAGAGGAAACTAATGCCGACAATTAAAGAAGAAATGGAACGTCGTCAGCTACTTTACAGCCTTCTCATGCCCATCATGAACAAGTATGTGCCGGGGTTGGACAAAGGGAAAGGACTTTACTTTTTATTCGTGAAGGCAGAAACAAAGACCCCTGGAGGACTACTGGCACGTCCGGTACTCACTAGCTACTATAAGAGTGACATCTTCAAGACCAGACCATATGATCCTTTCAATGACTACTCTAGCCCAAACGAGGCCATTCTATGCGCCAATTCCTTCCAAAGCATGTACACTCAGATGTTATGTGGTTTATTGATGCGTGAGCAAGTCCTCCGTGTTGGCGCCGTCTTCGCCTCCGGTCTCCTCCGTGCCATTCACTTCCTCCAGCACAACTGGAAACAGCTTGCACACGACATCTCCACCGCAACCTTAAATCCCAAAATAACGGACTCTTGTCTCCGTGAATGTGTAGTCTCCAAATATTTAACAAACCCGAATCCGGAGCTGGCGGAATTCATCTCAAAGGAATGCTCGACGGAGGAGTGGGAAGGGATTATCACGAGAATATGGCCGAATACTAAATATTTGGATGTGATTGTGACTGGAGCCATGGCTCAGTATATTCCCACTCTCGAATTTTACAGCGGTGGTTTACCCATGGCTTGCACTATGTATGCTTCTTCCGAGTGTTATTTTGGGGTAAATTTAAACCCAATGTGTAAACCCTCGGATGTGACTTACACCATCATGCCAAACATGTGCTACTGCGAATTCATCCCCCTCGATAACGATATGTCGTCTCCTCCAACTCAACTGGTTGACCTCGCTGACGTGGAAGTAGGGAAAGAGTACGAGCTCGTGATCACAACATACTCTGGACTCTGTCGTTACCGAGTCGGTGACATCCTACACGTCACTGGGTTCCACAACGCAGCCCCACAGTTTCGATTCGTGAGGAGGAAGAATGTTCTTTTGAGCATTGATTCCGACAAGACGGATGAAGCCGAGTTACAAAAGGCCATAGACAACGCCGCGCTGTTGTTGCGGGGGTTTAACACTAGCGTGGTGGAGTATACGAGCCATGCGGATACGAAAACAATCCCGGGACATTACGTCATTTATTGGGAGCTTCTTGTGAAAGACGAAGAAACTGGAAATTTCCCTCCAGGAGAGATTTTAAATCAGTGTTGTTTGGCTATTGAAGAGAGTTTGAACTCGGTTTACCGACAAGGCCGAGTTGCGGATAACTCAATTGGACCGTTGGAGATTAGGGTGGTGAGAAATGGGACATTTGAAGAGCTAATGGACTATGCGATCTCGAGGGGTGCGTCGATCAACCAGTACAAGGCGCCACGGTGCGTAAACTTCACGCCCATTATCGAGCTTCTCGACTCTAGGGTGACTTCTGTTCACTTTAGTCCATCCAAGCCACACTGGACACCAGAACGACGCGGCTAG

Protein sequence

MAVSSSVSHAKALQFIEEITTNADSVQQKVLAEILNRNSDTEYLKRFRLSRTASRDEFKSNVRVVTYEDLQLDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKEEMERRQLLYSLLMPIMNKYVPGLDKGKGLYFLFVKAETKTPGGLLARPVLTSYYKSDIFKTRPYDPFNDYSSPNEAILCANSFQSMYTQMLCGLLMREQVLRVGAVFASGLLRAIHFLQHNWKQLAHDISTATLNPKITDSCLRECVVSKYLTNPNPELAEFISKECSTEEWEGIITRIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMCYCEFIPLDNDMSSPPTQLVDLADVEVGKEYELVITTYSGLCRYRVGDILHVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAIDNAALLLRGFNTSVVEYTSHADTKTIPGHYVIYWELLVKDEETGNFPPGEILNQCCLAIEESLNSVYRQGRVADNSIGPLEIRVVRNGTFEELMDYAISRGASINQYKAPRCVNFTPIIELLDSRVTSVHFSPSKPHWTPERRG*
Homology
BLAST of Chy2G031400 vs. ExPASy Swiss-Prot
Match: O82333 (Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Arabidopsis thaliana OX=3702 GN=GH3.1 PE=2 SV=1)

HSP 1 Score: 885.9 bits (2288), Expect = 2.4e-256
Identity = 440/598 (73.58%), Postives = 501/598 (83.78%), Query Frame = 0

Query: 1   MAVSSSVS----------HAKALQFIEEITTNADSVQQKVLAEILNRNSDTEYLKRFRLS 60
           MAV S++S           AKAL+FIEE+T NAD+VQ+ +LAEIL RN+DTEYL+RF L 
Sbjct: 1   MAVDSNLSSPLGPPACEKDAKALRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLC 60

Query: 61  RTASRDEFKSNVRVVTYEDLQLDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPT 120
               RD FK+ + V+TYEDLQ +IQRIA+GDRSPI S+HPISEFLTSSGTSAGERKLMPT
Sbjct: 61  GATDRDTFKTKIPVITYEDLQPEIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEEMERRQLLYSLLMPIMNKYVPGLDKGKGLYFLFVKAETKTPGGLLARPVLTSYYKSD 180
           IKEE++RRQLLYSLLMP+MN YVPGLDKGKG+YFLFVK+ETKTPGGL ARPVLTSYYKS+
Sbjct: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSE 180

Query: 181 IFKTRPYDPFNDYSSPNEAILCANSFQSMYTQMLCGLLMREQVLRVGAVFASGLLRAIHF 240
            F++RPYDP+N Y+SPNEAILC +SFQSMYTQMLCGLL R  VLRVGAVFASGLLRAI F
Sbjct: 181 HFRSRPYDPYNVYTSPNEAILCPDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRF 240

Query: 241 LQHNWKQLAHDISTATLNPKITDSCLRECVVSKYLTNPNPELAEFISKECSTEEWEGIIT 300
           LQ +W + AHDI    L+ +ITD  +R+C+    +  P+P LAEFI +EC ++ WE IIT
Sbjct: 241 LQLHWSRFAHDIELGCLDSEITDPSIRQCMSG--ILKPDPVLAEFIRRECKSDNWEKIIT 300

Query: 301 RIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTY 360
           RIWPNTKYLDVIVTGAMAQYIPTLE+YSGGLPMACTMYASSECYFG+NLNPM KPS+V+Y
Sbjct: 301 RIWPNTKYLDVIVTGAMAQYIPTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSY 360

Query: 361 TIMPNMCYCEFIPLDNDMSSPPTQLVDLADVEVGKEYELVITTYSGLCRYRVGDILHVTG 420
           TIMPNM Y EFIPL        T+ V+L DV +GKEYELV+TTY+GLCRYRVGDIL VTG
Sbjct: 361 TIMPNMAYFEFIPLGG------TKAVELVDVNIGKEYELVVTTYAGLCRYRVGDILRVTG 420

Query: 421 FHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAIDNAALLL-RGFNTSVVEYTSHADTKT 480
           FHN+APQF FVRRKNVLLSIDSDKTDE+ELQKA++NA+ +L     + V EYTS+ADT T
Sbjct: 421 FHNSAPQFHFVRRKNVLLSIDSDKTDESELQKAVENASSILHEECGSRVAEYTSYADTST 480

Query: 481 IPGHYVIYWELLVKDEETGNFPPGEILNQCCLAIEESLNSVYRQGRVADNSIGPLEIRVV 540
           IPGHYV+YWELLV+D      P  E L +CCL +EESLNSVYRQ RVADNS+GPLEIRVV
Sbjct: 481 IPGHYVLYWELLVRD--GARQPSHETLTRCCLGMEESLNSVYRQSRVADNSVGPLEIRVV 540

Query: 541 RNGTFEELMDYAISRGASINQYKAPRCVNFTPIIELLDSRVTSVHFSPSKPHWTPERR 588
           RNGTFEELMDYAISRGASINQYK PRCVNFTPI+ELLDSRV S HFSPS PHWTPERR
Sbjct: 541 RNGTFEELMDYAISRGASINQYKVPRCVNFTPIVELLDSRVVSAHFSPSLPHWTPERR 588

BLAST of Chy2G031400 vs. ExPASy Swiss-Prot
Match: O22190 (Indole-3-acetic acid-amido synthetase GH3.3 OS=Arabidopsis thaliana OX=3702 GN=GH3.3 PE=1 SV=1)

HSP 1 Score: 869.0 bits (2244), Expect = 3.1e-251
Identity = 435/588 (73.98%), Postives = 501/588 (85.20%), Query Frame = 0

Query: 1   MAVSSSVSHAKALQFIEEITTNADSVQQKVLAEILNRNSDTEYLKRFRLSRTASRDEFKS 60
           M  S S    KAL+FIEE+T N D VQ+KV+ EIL+RNSDTEYLKRF L     R  FK+
Sbjct: 11  MMHSPSTKDVKALRFIEEMTRNVDFVQKKVIREILSRNSDTEYLKRFGLKGFTDRKTFKT 70

Query: 61  NVRVVTYEDLQLDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKEEMERRQL 120
            V VV Y+DL+ +IQRIANGDRS I SS+PI+EFLTSSGTSAGERKLMPTI E+M+RRQL
Sbjct: 71  KVPVVIYDDLKPEIQRIANGDRSMILSSYPITEFLTSSGTSAGERKLMPTIDEDMDRRQL 130

Query: 121 LYSLLMPIMNKYVPGLDKGKGLYFLFVKAETKTPGGLLARPVLTSYYKSDIFKTRPYDPF 180
           LYSLLMP+MN YVPGLDKGK LYFLFVK E+KTPGGL ARPVLTSYYKS+ FK RP DP+
Sbjct: 131 LYSLLMPVMNLYVPGLDKGKALYFLFVKTESKTPGGLPARPVLTSYYKSEQFKRRPNDPY 190

Query: 181 NDYSSPNEAILCANSFQSMYTQMLCGLLMREQVLRVGAVFASGLLRAIHFLQHNWKQLAH 240
           N Y+SPNEAILC +S QSMYTQMLCGLLMR +VLR+GAVFASGLLRAI FLQ NWK+LA 
Sbjct: 191 NVYTSPNEAILCPDSSQSMYTQMLCGLLMRHEVLRLGAVFASGLLRAIGFLQTNWKELAD 250

Query: 241 DISTATLNPKITDSCLRECVVSKYLTNPNPELAEFISKECSTE-EWEGIITRIWPNTKYL 300
           DIST TL+ +I+D  ++E  +SK LT P+ ELA+FI+  C  +  WEGIIT+IWPNTKYL
Sbjct: 251 DISTGTLSSRISDPAIKES-MSKILTKPDQELADFITSVCGQDNSWEGIITKIWPNTKYL 310

Query: 301 DVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMCYC 360
           DVIVTGAMAQYIP LE+YSGGLPMACTMYASSE YFG+NL PMCKPS+V+YTIMPNM Y 
Sbjct: 311 DVIVTGAMAQYIPMLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIMPNMAYF 370

Query: 361 EFIPLDNDMSSPPTQLVDLADVEVGKEYELVITTYSGLCRYRVGDILHVTGFHNAAPQFR 420
           EF+P  +++ +  ++LV+LADVEVGKEYELVITTY+GL RYRVGDIL VTGF+N+APQF+
Sbjct: 371 EFLP-HHEVPTEKSELVELADVEVGKEYELVITTYAGLNRYRVGDILQVTGFYNSAPQFK 430

Query: 421 FVRRKNVLLSIDSDKTDEAELQKAIDNAALLLRGFNTSVVEYTSHADTKTIPGHYVIYWE 480
           FVRRKNVLLSI+SDKTDEAELQ A++NA+LLL    T V+EYTS+A+TKTIPGHYVIYWE
Sbjct: 431 FVRRKNVLLSIESDKTDEAELQSAVENASLLLGEQGTRVIEYTSYAETKTIPGHYVIYWE 490

Query: 481 LLVKDEETGNFPPGEILNQCCLAIEESLNSVYRQGRVADNSIGPLEIRVVRNGTFEELMD 540
           LLVKD+   N P  E++ +CCL +EESLNSVYRQ RVAD SIGPLEIRVV+NGTFEELMD
Sbjct: 491 LLVKDQT--NPPNDEVMARCCLEMEESLNSVYRQSRVADKSIGPLEIRVVKNGTFEELMD 550

Query: 541 YAISRGASINQYKAPRCVNFTPIIELLDSRVTSVHFSPSKPHWTPERR 588
           YAISRGASINQYK PRCV+FTPI+ELLDSRV S HFSP+ PHW+PERR
Sbjct: 551 YAISRGASINQYKVPRCVSFTPIMELLDSRVVSTHFSPALPHWSPERR 594

BLAST of Chy2G031400 vs. ExPASy Swiss-Prot
Match: A3BLS0 (Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. indica OX=39946 GN=GH3.8 PE=1 SV=2)

HSP 1 Score: 848.6 bits (2191), Expect = 4.3e-245
Identity = 420/577 (72.79%), Postives = 477/577 (82.67%), Query Frame = 0

Query: 13  LQFIEEITTNADSVQQKVLAEILNRNSDTEYLKRFRLSRTASRDEFKSNVRVVTYEDLQL 72
           L+FI+E+TTN D+VQ++VL EIL RN+ TEYL +  L     R  F++ V VV+Y+DLQ 
Sbjct: 30  LRFIDEMTTNVDAVQERVLGEILGRNAGTEYLTKCGLDGATDRAAFRAKVPVVSYDDLQP 89

Query: 73  DIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKEEMERRQLLYSLLMPIMNKY 132
            IQRIANGDRSPI S+HP+SEFLTSSGTSAGERKLMPTI +E++RRQLLYSLLMP+MN Y
Sbjct: 90  YIQRIANGDRSPILSTHPVSEFLTSSGTSAGERKLMPTIMDELDRRQLLYSLLMPVMNLY 149

Query: 133 VPGLDKGKGLYFLFVKAETKTPGGLLARPVLTSYYKSDIFKTRPYDPFNDYSSPNEAILC 192
           VPGLDKGKGLYFLFVK+ETKTPGGL ARPVLTSYYKSD FK RPYDP+++Y+SP  AILC
Sbjct: 150 VPGLDKGKGLYFLFVKSETKTPGGLTARPVLTSYYKSDHFKNRPYDPYHNYTSPTAAILC 209

Query: 193 ANSFQSMYTQMLCGLLMREQVLRVGAVFASGLLRAIHFLQHNWKQLAHDISTATLNPKIT 252
           A++FQSMY QM+CGL  R  VLR+GAVFASGLLRAI FLQ NW+QLA DI +  L P++T
Sbjct: 210 ADAFQSMYAQMVCGLCQRNDVLRLGAVFASGLLRAIRFLQLNWEQLADDIESGELTPRVT 269

Query: 253 DSCLRECVVSKYLTNPNPELAEFISKECSTEEWEGIITRIWPNTKYLDVIVTGAMAQYIP 312
           D  +RE V +  L  P+PELA+ I  ECS  +W GIITR+WPNTKYLDVIVTGAMAQYIP
Sbjct: 270 DPSVREAVAAILL--PDPELAKLIRAECSKGDWAGIITRVWPNTKYLDVIVTGAMAQYIP 329

Query: 313 TLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMCYCEFIPLDND--MSS 372
           TLEFYSGGLPMACTMYASSECYFG+NL PMC PS+V+YTIMPNM Y EF+P+D     S 
Sbjct: 330 TLEFYSGGLPMACTMYASSECYFGLNLRPMCDPSEVSYTIMPNMGYFEFLPVDETGAASG 389

Query: 373 PPTQLVDLADVEVGKEYELVITTYSGLCRYRVGDILHVTGFHNAAPQFRFVRRKNVLLSI 432
             TQLVDLA VEVG+EYELVITTY+GL RYRVGD+L VTGFHNAAPQFRFVRRKNVLLSI
Sbjct: 390 DATQLVDLARVEVGREYELVITTYAGLNRYRVGDVLRVTGFHNAAPQFRFVRRKNVLLSI 449

Query: 433 DSDKTDEAELQKAIDNAALLLRGFNTSVVEYTSHADTKTIPGHYVIYWELLVKDEETGNF 492
           +SDKTDEAELQ+A++ A+ LLR    SVVEYTS A TK IPGHYVIYWELL K       
Sbjct: 450 ESDKTDEAELQRAVERASALLRPHGASVVEYTSQACTKRIPGHYVIYWELLTKG-AGATV 509

Query: 493 PPGEILNQCCLAIEESLNSVYRQGRVADNSIGPLEIRVVRNGTFEELMDYAISRGASINQ 552
              + L +CCL +EE+LN+VYRQ RVAD SIGPLEIRVVR GTFEELMDYAISRGASINQ
Sbjct: 510 VDADTLGRCCLEMEEALNTVYRQSRVADGSIGPLEIRVVRPGTFEELMDYAISRGASINQ 569

Query: 553 YKAPRCVNFTPIIELLDSRVTSVHFSPSKPHWTPERR 588
           YK PRCV F PI+ELLDSRV S HFSP+ PHWTP RR
Sbjct: 570 YKVPRCVTFPPIVELLDSRVVSSHFSPALPHWTPARR 603

BLAST of Chy2G031400 vs. ExPASy Swiss-Prot
Match: Q0D4Z6 (Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.8 PE=1 SV=1)

HSP 1 Score: 848.6 bits (2191), Expect = 4.3e-245
Identity = 420/577 (72.79%), Postives = 477/577 (82.67%), Query Frame = 0

Query: 13  LQFIEEITTNADSVQQKVLAEILNRNSDTEYLKRFRLSRTASRDEFKSNVRVVTYEDLQL 72
           L+FI+E+TTN D+VQ++VL EIL RN+ TEYL +  L     R  F++ V VV+Y+DLQ 
Sbjct: 30  LRFIDEMTTNVDAVQERVLGEILGRNAGTEYLTKCGLDGATDRAAFRAKVPVVSYDDLQP 89

Query: 73  DIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKEEMERRQLLYSLLMPIMNKY 132
            IQRIANGDRSPI S+HP+SEFLTSSGTSAGERKLMPTI +E++RRQLLYSLLMP+MN Y
Sbjct: 90  YIQRIANGDRSPILSTHPVSEFLTSSGTSAGERKLMPTIMDELDRRQLLYSLLMPVMNLY 149

Query: 133 VPGLDKGKGLYFLFVKAETKTPGGLLARPVLTSYYKSDIFKTRPYDPFNDYSSPNEAILC 192
           VPGLDKGKGLYFLFVK+ETKTPGGL ARPVLTSYYKSD FK RPYDP+++Y+SP  AILC
Sbjct: 150 VPGLDKGKGLYFLFVKSETKTPGGLTARPVLTSYYKSDHFKNRPYDPYHNYTSPTAAILC 209

Query: 193 ANSFQSMYTQMLCGLLMREQVLRVGAVFASGLLRAIHFLQHNWKQLAHDISTATLNPKIT 252
           A++FQSMY QM+CGL  R  VLR+GAVFASGLLRAI FLQ NW+QLA DI +  L P++T
Sbjct: 210 ADAFQSMYAQMVCGLCQRNDVLRLGAVFASGLLRAIRFLQLNWEQLADDIESGELTPRVT 269

Query: 253 DSCLRECVVSKYLTNPNPELAEFISKECSTEEWEGIITRIWPNTKYLDVIVTGAMAQYIP 312
           D  +RE V +  L  P+PELA+ I  ECS  +W GIITR+WPNTKYLDVIVTGAMAQYIP
Sbjct: 270 DPSVREAVAAILL--PDPELAKLIRAECSKGDWAGIITRVWPNTKYLDVIVTGAMAQYIP 329

Query: 313 TLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMCYCEFIPLDND--MSS 372
           TLEFYSGGLPMACTMYASSECYFG+NL PMC PS+V+YTIMPNM Y EF+P+D     S 
Sbjct: 330 TLEFYSGGLPMACTMYASSECYFGLNLRPMCDPSEVSYTIMPNMGYFEFLPVDETGAASG 389

Query: 373 PPTQLVDLADVEVGKEYELVITTYSGLCRYRVGDILHVTGFHNAAPQFRFVRRKNVLLSI 432
             TQLVDLA VEVG+EYELVITTY+GL RYRVGD+L VTGFHNAAPQFRFVRRKNVLLSI
Sbjct: 390 DATQLVDLARVEVGREYELVITTYAGLNRYRVGDVLRVTGFHNAAPQFRFVRRKNVLLSI 449

Query: 433 DSDKTDEAELQKAIDNAALLLRGFNTSVVEYTSHADTKTIPGHYVIYWELLVKDEETGNF 492
           +SDKTDEAELQ+A++ A+ LLR    SVVEYTS A TK IPGHYVIYWELL K       
Sbjct: 450 ESDKTDEAELQRAVERASALLRPHGASVVEYTSQACTKRIPGHYVIYWELLTKG-AGATV 509

Query: 493 PPGEILNQCCLAIEESLNSVYRQGRVADNSIGPLEIRVVRNGTFEELMDYAISRGASINQ 552
              + L +CCL +EE+LN+VYRQ RVAD SIGPLEIRVVR GTFEELMDYAISRGASINQ
Sbjct: 510 VDADTLGRCCLEMEEALNTVYRQSRVADGSIGPLEIRVVRPGTFEELMDYAISRGASINQ 569

Query: 553 YKAPRCVNFTPIIELLDSRVTSVHFSPSKPHWTPERR 588
           YK PRCV F PI+ELLDSRV S HFSP+ PHWTP RR
Sbjct: 570 YKVPRCVTFPPIVELLDSRVVSSHFSPALPHWTPARR 603

BLAST of Chy2G031400 vs. ExPASy Swiss-Prot
Match: Q9LQ68 (Indole-3-acetic acid-amido synthetase GH3.4 OS=Arabidopsis thaliana OX=3702 GN=GH3.4 PE=1 SV=1)

HSP 1 Score: 833.2 bits (2151), Expect = 1.9e-240
Identity = 416/586 (70.99%), Postives = 486/586 (82.94%), Query Frame = 0

Query: 4   SSSVSHAKALQFIEEITTNADSVQQKVLAEILNRNSDTEYLKRFRLSRTASRDEFKSNVR 63
           ++S +  KAL+FIEEIT N DSVQ+KVL EIL+RNS+TEYLKRF L+    R  FKS V 
Sbjct: 15  TTSETEVKALKFIEEITRNPDSVQEKVLGEILSRNSNTEYLKRFDLNGAVDRKSFKSKVP 74

Query: 64  VVTYEDLQLDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKEEMERRQLLYS 123
           VV YEDL+ DIQRI+NGDRSPI SSHPI+EFLTSSGTSAGERKLMPTI+E++ RRQLL +
Sbjct: 75  VVIYEDLKTDIQRISNGDRSPILSSHPITEFLTSSGTSAGERKLMPTIEEDINRRQLLGN 134

Query: 124 LLMPIMNKYVPGLDKGKGLYFLFVKAETKTPGGLLARPVLTSYYKSDIFKTRPYDPFNDY 183
           LLMP+MN YVPGLDKGKGLYFLFVK+E+ T GGL ARP LTSYYKSD F+T   D  + Y
Sbjct: 135 LLMPVMNLYVPGLDKGKGLYFLFVKSESTTSGGLPARPALTSYYKSDYFRTSDSD--SVY 194

Query: 184 SSPNEAILCANSFQSMYTQMLCGLLMREQVLRVGAVFASGLLRAIHFLQHNWKQLAHDIS 243
           +SP EAILC +S QSMYTQMLCGLLMR +V R+GAVF SGLLRAI FLQ+NWK+L+ DIS
Sbjct: 195 TSPKEAILCCDSSQSMYTQMLCGLLMRHEVNRLGAVFPSGLLRAISFLQNNWKELSQDIS 254

Query: 244 TATLNPKITDSCLRECVVSKYLTNPNPELAEFISKECSTEEWEGIITRIWPNTKYLDVIV 303
           T TL+ KI D  ++   +S  L  P+ ELAEF+   CS E WEGIIT+IWPNTKYLDVIV
Sbjct: 255 TGTLSSKIFDHAIK-TRMSNILNKPDQELAEFLIGVCSQENWEGIITKIWPNTKYLDVIV 314

Query: 304 TGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMCYCEFIP 363
           TGAMA+YIP LE+YSGGLPMA  +YASSE YFG+NLNPMCKPS+V+YTI PNM Y EF+P
Sbjct: 315 TGAMAEYIPMLEYYSGGLPMASMIYASSESYFGINLNPMCKPSEVSYTIFPNMAYFEFLP 374

Query: 364 --LDNDMSSPPTQLVDLADVEVGKEYELVITTYSGLCRYRVGDILHVTGFHNAAPQFRFV 423
              D D     T LV+LADVEVGKEYELVITTY+GL RYRVGDIL VTGFHN+APQF+F+
Sbjct: 375 HNHDGDGGVEATSLVELADVEVGKEYELVITTYAGLYRYRVGDILRVTGFHNSAPQFKFI 434

Query: 424 RRKNVLLSIDSDKTDEAELQKAIDNAALLLRGFNTSVVEYTSHADTKTIPGHYVIYWELL 483
           RR+NVLLSI+SDKTDEA+LQKA++NA+ LL    T V+EYTS+ADTKTIPGHYVIYWELL
Sbjct: 435 RRENVLLSIESDKTDEADLQKAVENASRLLAEQGTRVIEYTSYADTKTIPGHYVIYWELL 494

Query: 484 VKDEETGNFPPGEILNQCCLAIEESLNSVYRQGRVADNSIGPLEIRVVRNGTFEELMDYA 543
            +D+     P  E++ +CCL +EESLN+VYRQ RV+D SIGPLEIRVV+NGTFEELMD++
Sbjct: 495 SRDQSNA-LPSDEVMAKCCLEMEESLNAVYRQSRVSDKSIGPLEIRVVQNGTFEELMDFS 554

Query: 544 ISRGASINQYKAPRCVNFTPIIELLDSRVTSVHFSPSKPHWTPERR 588
           ISRG+SINQYK PRCV+ TPI++LLDSRV S HFSPS PHW+PERR
Sbjct: 555 ISRGSSINQYKVPRCVSLTPIMKLLDSRVVSAHFSPSLPHWSPERR 596

BLAST of Chy2G031400 vs. ExPASy TrEMBL
Match: A0A0A0KLF8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G492310 PE=3 SV=1)

HSP 1 Score: 1176.0 bits (3041), Expect = 0.0e+00
Identity = 579/588 (98.47%), Postives = 583/588 (99.15%), Query Frame = 0

Query: 1   MAVSSSVSHAKALQFIEEITTNADSVQQKVLAEILNRNSDTEYLKRFRLSRTASRDEFKS 60
           MAVSSSVSHAKALQFIEEITTNADSVQQKVLAEILNRNSDTEYLKRF+LSRTASRDEFKS
Sbjct: 1   MAVSSSVSHAKALQFIEEITTNADSVQQKVLAEILNRNSDTEYLKRFQLSRTASRDEFKS 60

Query: 61  NVRVVTYEDLQLDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKEEMERRQL 120
           NVRVVTYEDLQ DIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKEEMERRQL
Sbjct: 61  NVRVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKEEMERRQL 120

Query: 121 LYSLLMPIMNKYVPGLDKGKGLYFLFVKAETKTPGGLLARPVLTSYYKSDIFKTRPYDPF 180
           LYSLLMPIMNKYVPGLDKGKGLYFLFVKAETKT GGLLARPVLTSYYKSDIFKTRPYDPF
Sbjct: 121 LYSLLMPIMNKYVPGLDKGKGLYFLFVKAETKTTGGLLARPVLTSYYKSDIFKTRPYDPF 180

Query: 181 NDYSSPNEAILCANSFQSMYTQMLCGLLMREQVLRVGAVFASGLLRAIHFLQHNWKQLAH 240
           NDY+SPNEAILCANSFQSMYTQMLCGLLMREQVLRVGAVFASGLLRAIHFLQHNWKQLAH
Sbjct: 181 NDYTSPNEAILCANSFQSMYTQMLCGLLMREQVLRVGAVFASGLLRAIHFLQHNWKQLAH 240

Query: 241 DISTATLNPKITDSCLRECVVSKYLTNPNPELAEFISKECSTEEWEGIITRIWPNTKYLD 300
           DISTATLNPKITD CLREC+VSKYLTNPNPELAE ISKECSTEEWEGIITRIWPNTKYLD
Sbjct: 241 DISTATLNPKITDPCLRECIVSKYLTNPNPELAELISKECSTEEWEGIITRIWPNTKYLD 300

Query: 301 VIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMCYCE 360
           VIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMCYCE
Sbjct: 301 VIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMCYCE 360

Query: 361 FIPLDNDMSSPPTQLVDLADVEVGKEYELVITTYSGLCRYRVGDILHVTGFHNAAPQFRF 420
           FIPLDNDMSS PTQLVDLADVEVGKEYELVITTYSGLCRYRVGDILHVTGFHNAAPQFRF
Sbjct: 361 FIPLDNDMSSSPTQLVDLADVEVGKEYELVITTYSGLCRYRVGDILHVTGFHNAAPQFRF 420

Query: 421 VRRKNVLLSIDSDKTDEAELQKAIDNAALLLRGFNTSVVEYTSHADTKTIPGHYVIYWEL 480
           VRRKNVLLSIDSDKTDEAELQKAIDNAALLLRGFNTSVVEYTS+ADTKTIPGHYVIYWEL
Sbjct: 421 VRRKNVLLSIDSDKTDEAELQKAIDNAALLLRGFNTSVVEYTSYADTKTIPGHYVIYWEL 480

Query: 481 LVKDEETGNFPPGEILNQCCLAIEESLNSVYRQGRVADNSIGPLEIRVVRNGTFEELMDY 540
           LVKDEETGNFPPGEILNQCCLAIEESLNSVYRQGRVADNSIGPLEIRVVRNGTFEELMDY
Sbjct: 481 LVKDEETGNFPPGEILNQCCLAIEESLNSVYRQGRVADNSIGPLEIRVVRNGTFEELMDY 540

Query: 541 AISRGASINQYKAPRCVNFTPIIELLDSRVTSVHFSPSKPHWTPERRG 589
           AISRGASINQYKAPRCVNFTPIIELLDSRVTSVHFSPSKPHWTPERRG
Sbjct: 541 AISRGASINQYKAPRCVNFTPIIELLDSRVTSVHFSPSKPHWTPERRG 588

BLAST of Chy2G031400 vs. ExPASy TrEMBL
Match: A0A5D3CR82 (Putative indole-3-acetic acid-amido synthetase GH3.1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G004410 PE=3 SV=1)

HSP 1 Score: 1154.0 bits (2984), Expect = 0.0e+00
Identity = 571/588 (97.11%), Postives = 575/588 (97.79%), Query Frame = 0

Query: 1   MAVSSSVSHAKALQFIEEITTNADSVQQKVLAEILNRNSDTEYLKRFRLSRTASRDEFKS 60
           MA SSSVSHAKALQFIEEITTNADSVQQKVLAEILNRNSDTEYLKRFRLSR A RDEFKS
Sbjct: 1   MAASSSVSHAKALQFIEEITTNADSVQQKVLAEILNRNSDTEYLKRFRLSRAAGRDEFKS 60

Query: 61  NVRVVTYEDLQLDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKEEMERRQL 120
           NVRVVTYEDLQ DIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKEEMERRQL
Sbjct: 61  NVRVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKEEMERRQL 120

Query: 121 LYSLLMPIMNKYVPGLDKGKGLYFLFVKAETKTPGGLLARPVLTSYYKSDIFKTRPYDPF 180
           LYSLLMPIMNKYVPGLDKGKGLYFLFVKAETKTPGGL ARPVLTSYYKSDIF TRPYDPF
Sbjct: 121 LYSLLMPIMNKYVPGLDKGKGLYFLFVKAETKTPGGLPARPVLTSYYKSDIFMTRPYDPF 180

Query: 181 NDYSSPNEAILCANSFQSMYTQMLCGLLMREQVLRVGAVFASGLLRAIHFLQHNWKQLAH 240
           NDY+SPNEAILCANSFQSMYTQMLCGLLMR+QVLRVGAVFASGLLRAIHFLQ NWKQLAH
Sbjct: 181 NDYTSPNEAILCANSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAIHFLQLNWKQLAH 240

Query: 241 DISTATLNPKITDSCLRECVVSKYLTNPNPELAEFISKECSTEEWEGIITRIWPNTKYLD 300
           DISTATLNPKITDSCLREC+VSKYLTNPN ELAE ISKECSTEEWEGIITRIWPNTKYLD
Sbjct: 241 DISTATLNPKITDSCLRECIVSKYLTNPNSELAELISKECSTEEWEGIITRIWPNTKYLD 300

Query: 301 VIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMCYCE 360
           VIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMCY E
Sbjct: 301 VIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMCYFE 360

Query: 361 FIPLDNDMSSPPTQLVDLADVEVGKEYELVITTYSGLCRYRVGDILHVTGFHNAAPQFRF 420
           FIPLDNDMSSPPTQLVDLADVEVGKEYELVITTYSGLCRYRVGDILHVTGFHNAAPQFRF
Sbjct: 361 FIPLDNDMSSPPTQLVDLADVEVGKEYELVITTYSGLCRYRVGDILHVTGFHNAAPQFRF 420

Query: 421 VRRKNVLLSIDSDKTDEAELQKAIDNAALLLRGFNTSVVEYTSHADTKTIPGHYVIYWEL 480
           VRRKNVLLSIDSDKTDEAELQKAIDNAALLLRGFNTSVVEYTS+ADTKTIPGHYVIYWEL
Sbjct: 421 VRRKNVLLSIDSDKTDEAELQKAIDNAALLLRGFNTSVVEYTSYADTKTIPGHYVIYWEL 480

Query: 481 LVKDEETGNFPPGEILNQCCLAIEESLNSVYRQGRVADNSIGPLEIRVVRNGTFEELMDY 540
           LVKDEETGNFPPGEILNQ CLAIEESLNSVYRQGRVADNSIGPLEIRVVR GTFEELMDY
Sbjct: 481 LVKDEETGNFPPGEILNQSCLAIEESLNSVYRQGRVADNSIGPLEIRVVRKGTFEELMDY 540

Query: 541 AISRGASINQYKAPRCVNFTPIIELLDSRVTSVHFSPSKPHWTPERRG 589
           AISRGASINQYKAPRCVNFTPIIELLDSRVTSVHFSPSKPHWTPE RG
Sbjct: 541 AISRGASINQYKAPRCVNFTPIIELLDSRVTSVHFSPSKPHWTPEPRG 588

BLAST of Chy2G031400 vs. ExPASy TrEMBL
Match: A0A1S3B1M0 (probable indole-3-acetic acid-amido synthetase GH3.1 OS=Cucumis melo OX=3656 GN=LOC103484815 PE=3 SV=1)

HSP 1 Score: 1151.7 bits (2978), Expect = 0.0e+00
Identity = 570/588 (96.94%), Postives = 575/588 (97.79%), Query Frame = 0

Query: 1   MAVSSSVSHAKALQFIEEITTNADSVQQKVLAEILNRNSDTEYLKRFRLSRTASRDEFKS 60
           MA SSSVSHAKALQFIEEITTNADSVQQKVLAEILNRNSDTEYLKRFRLSR+A RDEFKS
Sbjct: 1   MAASSSVSHAKALQFIEEITTNADSVQQKVLAEILNRNSDTEYLKRFRLSRSAGRDEFKS 60

Query: 61  NVRVVTYEDLQLDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKEEMERRQL 120
           NVRVVTYEDLQ DIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIK EMERRQL
Sbjct: 61  NVRVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKGEMERRQL 120

Query: 121 LYSLLMPIMNKYVPGLDKGKGLYFLFVKAETKTPGGLLARPVLTSYYKSDIFKTRPYDPF 180
           LYSLLMPIMNKYVPGLDKGKGLYFLFVKAETKTPGGL ARPVLTSYYKSDIF TRPYDPF
Sbjct: 121 LYSLLMPIMNKYVPGLDKGKGLYFLFVKAETKTPGGLPARPVLTSYYKSDIFMTRPYDPF 180

Query: 181 NDYSSPNEAILCANSFQSMYTQMLCGLLMREQVLRVGAVFASGLLRAIHFLQHNWKQLAH 240
           NDY+SPNEAILCANSFQSMYTQMLCGLLMR+QVLRVGAVFASGLLRAIHFLQ NWKQLAH
Sbjct: 181 NDYTSPNEAILCANSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAIHFLQLNWKQLAH 240

Query: 241 DISTATLNPKITDSCLRECVVSKYLTNPNPELAEFISKECSTEEWEGIITRIWPNTKYLD 300
           DISTATLNPKITDSCLREC+VSKYLTNPN ELAE ISKECSTEEWEGIITRIWPNTKYLD
Sbjct: 241 DISTATLNPKITDSCLRECIVSKYLTNPNSELAELISKECSTEEWEGIITRIWPNTKYLD 300

Query: 301 VIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMCYCE 360
           VIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMCY E
Sbjct: 301 VIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMCYFE 360

Query: 361 FIPLDNDMSSPPTQLVDLADVEVGKEYELVITTYSGLCRYRVGDILHVTGFHNAAPQFRF 420
           FIPLDNDMSSPPTQLVDLADVEVGKEYELVITTYSGLCRYRVGDILHVTGFHNAAPQFRF
Sbjct: 361 FIPLDNDMSSPPTQLVDLADVEVGKEYELVITTYSGLCRYRVGDILHVTGFHNAAPQFRF 420

Query: 421 VRRKNVLLSIDSDKTDEAELQKAIDNAALLLRGFNTSVVEYTSHADTKTIPGHYVIYWEL 480
           VRRKNVLLSIDSDKTDEAELQKAIDNAALLLRGFNTSVVEYTS+ADTKTIPGHYVIYWEL
Sbjct: 421 VRRKNVLLSIDSDKTDEAELQKAIDNAALLLRGFNTSVVEYTSYADTKTIPGHYVIYWEL 480

Query: 481 LVKDEETGNFPPGEILNQCCLAIEESLNSVYRQGRVADNSIGPLEIRVVRNGTFEELMDY 540
           LVKDEETGNFPPGEILNQ CLAIEESLNSVYRQGRVADNSIGPLEIRVVR GTFEELMDY
Sbjct: 481 LVKDEETGNFPPGEILNQSCLAIEESLNSVYRQGRVADNSIGPLEIRVVRKGTFEELMDY 540

Query: 541 AISRGASINQYKAPRCVNFTPIIELLDSRVTSVHFSPSKPHWTPERRG 589
           AISRGASINQYKAPRCVNFTPIIELLDSRVTSVHFSPSKPHWTPE RG
Sbjct: 541 AISRGASINQYKAPRCVNFTPIIELLDSRVTSVHFSPSKPHWTPEPRG 588

BLAST of Chy2G031400 vs. ExPASy TrEMBL
Match: A0A5D3CNV8 (Putative indole-3-acetic acid-amido synthetase GH3.1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G004400 PE=3 SV=1)

HSP 1 Score: 1043.9 bits (2698), Expect = 2.6e-301
Identity = 516/585 (88.21%), Postives = 541/585 (92.48%), Query Frame = 0

Query: 10  AKALQFIEEITTNADSVQQKVLAEILNRNSDTEYLKRFRLSRTASRDEFKSNVRVVTYED 69
           AKAL+FIEE TTNAD VQQ+VLAEIL +N+ TEY+ RFRL+    RD FKS   VVTYED
Sbjct: 22  AKALRFIEETTTNADLVQQRVLAEILTQNAHTEYVNRFRLNGATDRDTFKSKFPVVTYED 81

Query: 70  LQLDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKEEMERRQLLYSLLMPIM 129
           LQ DIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKEEMERRQLLYSLLMPIM
Sbjct: 82  LQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKEEMERRQLLYSLLMPIM 141

Query: 130 NKYVPGLDKGKGLYFLFVKAETKTPGGLLARPVLTSYYKSDIFKTRPYDPFNDYSSPNEA 189
           N YVPGLDKGKGLYFLFVKAETKTPGGL+ARPVLTSYYKSDIFKTRPYDPFNDY+SPNEA
Sbjct: 142 NLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDIFKTRPYDPFNDYTSPNEA 201

Query: 190 ILCANSFQSMYTQMLCGLLMREQVLRVGAVFASGLLRAIHFLQHNWKQLAHDISTATLNP 249
           +LCA+SFQSMYTQMLCGLLMR+QVLRVGAVFASGLLRAI FLQ NWKQLAHDIST TLNP
Sbjct: 202 VLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAIRFLQLNWKQLAHDISTGTLNP 261

Query: 250 KITDSCLRECVVSKYLTNPNPELAEFISKECSTEEWEGIITRIWPNTKYLDVIVTGAMAQ 309
           KITD+CLREC+ SKYLTNPNPELAEFIS EC TEEWEGIITRIWPNTKYLDVIVTGAMAQ
Sbjct: 262 KITDTCLRECITSKYLTNPNPELAEFISNECYTEEWEGIITRIWPNTKYLDVIVTGAMAQ 321

Query: 310 YIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMCYCEFIPLDNDMS 369
           YIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNM Y EF+P D+   
Sbjct: 322 YIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMGYFEFLPHDSSSR 381

Query: 370 S------PPTQLVDLADVEVGKEYELVITTYSGLCRYRVGDILHVTGFHNAAPQFRFVRR 429
           +       P +LVDLADVEVGKEYELVITTY+GLCRYRVGD+L VTGFHNAAPQFRFVRR
Sbjct: 382 ASALSRDSPPRLVDLADVEVGKEYELVITTYAGLCRYRVGDVLEVTGFHNAAPQFRFVRR 441

Query: 430 KNVLLSIDSDKTDEAELQKAIDNAALLLRGFNTSVVEYTSHADTKTIPGHYVIYWELLVK 489
           KNVLLSIDSDKTDEAELQKA++NA+ LLR FNT+VVEYTS+ADTKTIPGHYVIYWELLVK
Sbjct: 442 KNVLLSIDSDKTDEAELQKAVENASSLLRDFNTTVVEYTSYADTKTIPGHYVIYWELLVK 501

Query: 490 DEETGN-FPPGEILNQCCLAIEESLNSVYRQGRVADNSIGPLEIRVVRNGTFEELMDYAI 549
           D ET N  P  EILNQCCLAIEESLNSVYRQGRVADNSIGPLEIRVVRNGTFEELMDYAI
Sbjct: 502 DNETMNSHPTDEILNQCCLAIEESLNSVYRQGRVADNSIGPLEIRVVRNGTFEELMDYAI 561

Query: 550 SRGASINQYKAPRCVNFTPIIELLDSRVTSVHFSPSKPHWTPERR 588
           SRGASINQYKAPRCVNFTPI+ELLDSRVTSVHFSPSKPHWTPERR
Sbjct: 562 SRGASINQYKAPRCVNFTPIVELLDSRVTSVHFSPSKPHWTPERR 606

BLAST of Chy2G031400 vs. ExPASy TrEMBL
Match: A0A1S3B0G2 (probable indole-3-acetic acid-amido synthetase GH3.1 OS=Cucumis melo OX=3656 GN=LOC103484816 PE=3 SV=1)

HSP 1 Score: 1043.9 bits (2698), Expect = 2.6e-301
Identity = 516/585 (88.21%), Postives = 541/585 (92.48%), Query Frame = 0

Query: 10  AKALQFIEEITTNADSVQQKVLAEILNRNSDTEYLKRFRLSRTASRDEFKSNVRVVTYED 69
           AKAL+FIEE TTNAD VQQ+VLAEIL +N+ TEY+ RFRL+    RD FKS   VVTYED
Sbjct: 22  AKALRFIEETTTNADLVQQRVLAEILTQNAHTEYVNRFRLNGATDRDTFKSKFPVVTYED 81

Query: 70  LQLDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKEEMERRQLLYSLLMPIM 129
           LQ DIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKEEMERRQLLYSLLMPIM
Sbjct: 82  LQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKEEMERRQLLYSLLMPIM 141

Query: 130 NKYVPGLDKGKGLYFLFVKAETKTPGGLLARPVLTSYYKSDIFKTRPYDPFNDYSSPNEA 189
           N YVPGLDKGKGLYFLFVKAETKTPGGL+ARPVLTSYYKSDIFKTRPYDPFNDY+SPNEA
Sbjct: 142 NLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDIFKTRPYDPFNDYTSPNEA 201

Query: 190 ILCANSFQSMYTQMLCGLLMREQVLRVGAVFASGLLRAIHFLQHNWKQLAHDISTATLNP 249
           +LCA+SFQSMYTQMLCGLLMR+QVLRVGAVFASGLLRAI FLQ NWKQLAHDIST TLNP
Sbjct: 202 VLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAIRFLQLNWKQLAHDISTGTLNP 261

Query: 250 KITDSCLRECVVSKYLTNPNPELAEFISKECSTEEWEGIITRIWPNTKYLDVIVTGAMAQ 309
           KITD+CLREC+ SKYLTNPNPELAEFIS EC TEEWEGIITRIWPNTKYLDVIVTGAMAQ
Sbjct: 262 KITDTCLRECITSKYLTNPNPELAEFISNECYTEEWEGIITRIWPNTKYLDVIVTGAMAQ 321

Query: 310 YIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMCYCEFIPLDNDMS 369
           YIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNM Y EF+P D+   
Sbjct: 322 YIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMGYFEFLPHDSSSR 381

Query: 370 S------PPTQLVDLADVEVGKEYELVITTYSGLCRYRVGDILHVTGFHNAAPQFRFVRR 429
           +       P +LVDLADVEVGKEYELVITTY+GLCRYRVGD+L VTGFHNAAPQFRFVRR
Sbjct: 382 ASALSRDSPPRLVDLADVEVGKEYELVITTYAGLCRYRVGDVLEVTGFHNAAPQFRFVRR 441

Query: 430 KNVLLSIDSDKTDEAELQKAIDNAALLLRGFNTSVVEYTSHADTKTIPGHYVIYWELLVK 489
           KNVLLSIDSDKTDEAELQKA++NA+ LLR FNT+VVEYTS+ADTKTIPGHYVIYWELLVK
Sbjct: 442 KNVLLSIDSDKTDEAELQKAVENASSLLRDFNTTVVEYTSYADTKTIPGHYVIYWELLVK 501

Query: 490 DEETGN-FPPGEILNQCCLAIEESLNSVYRQGRVADNSIGPLEIRVVRNGTFEELMDYAI 549
           D ET N  P  EILNQCCLAIEESLNSVYRQGRVADNSIGPLEIRVVRNGTFEELMDYAI
Sbjct: 502 DNETMNSHPTDEILNQCCLAIEESLNSVYRQGRVADNSIGPLEIRVVRNGTFEELMDYAI 561

Query: 550 SRGASINQYKAPRCVNFTPIIELLDSRVTSVHFSPSKPHWTPERR 588
           SRGASINQYKAPRCVNFTPI+ELLDSRVTSVHFSPSKPHWTPERR
Sbjct: 562 SRGASINQYKAPRCVNFTPIVELLDSRVTSVHFSPSKPHWTPERR 606

BLAST of Chy2G031400 vs. NCBI nr
Match: XP_004141994.1 (probable indole-3-acetic acid-amido synthetase GH3.1 [Cucumis sativus] >KGN48571.1 hypothetical protein Csa_003209 [Cucumis sativus])

HSP 1 Score: 1167 bits (3018), Expect = 0.0
Identity = 579/588 (98.47%), Postives = 583/588 (99.15%), Query Frame = 0

Query: 1   MAVSSSVSHAKALQFIEEITTNADSVQQKVLAEILNRNSDTEYLKRFRLSRTASRDEFKS 60
           MAVSSSVSHAKALQFIEEITTNADSVQQKVLAEILNRNSDTEYLKRF+LSRTASRDEFKS
Sbjct: 1   MAVSSSVSHAKALQFIEEITTNADSVQQKVLAEILNRNSDTEYLKRFQLSRTASRDEFKS 60

Query: 61  NVRVVTYEDLQLDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKEEMERRQL 120
           NVRVVTYEDLQ DIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKEEMERRQL
Sbjct: 61  NVRVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKEEMERRQL 120

Query: 121 LYSLLMPIMNKYVPGLDKGKGLYFLFVKAETKTPGGLLARPVLTSYYKSDIFKTRPYDPF 180
           LYSLLMPIMNKYVPGLDKGKGLYFLFVKAETKT GGLLARPVLTSYYKSDIFKTRPYDPF
Sbjct: 121 LYSLLMPIMNKYVPGLDKGKGLYFLFVKAETKTTGGLLARPVLTSYYKSDIFKTRPYDPF 180

Query: 181 NDYSSPNEAILCANSFQSMYTQMLCGLLMREQVLRVGAVFASGLLRAIHFLQHNWKQLAH 240
           NDY+SPNEAILCANSFQSMYTQMLCGLLMREQVLRVGAVFASGLLRAIHFLQHNWKQLAH
Sbjct: 181 NDYTSPNEAILCANSFQSMYTQMLCGLLMREQVLRVGAVFASGLLRAIHFLQHNWKQLAH 240

Query: 241 DISTATLNPKITDSCLRECVVSKYLTNPNPELAEFISKECSTEEWEGIITRIWPNTKYLD 300
           DISTATLNPKITD CLREC+VSKYLTNPNPELAE ISKECSTEEWEGIITRIWPNTKYLD
Sbjct: 241 DISTATLNPKITDPCLRECIVSKYLTNPNPELAELISKECSTEEWEGIITRIWPNTKYLD 300

Query: 301 VIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMCYCE 360
           VIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMCYCE
Sbjct: 301 VIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMCYCE 360

Query: 361 FIPLDNDMSSPPTQLVDLADVEVGKEYELVITTYSGLCRYRVGDILHVTGFHNAAPQFRF 420
           FIPLDNDMSS PTQLVDLADVEVGKEYELVITTYSGLCRYRVGDILHVTGFHNAAPQFRF
Sbjct: 361 FIPLDNDMSSSPTQLVDLADVEVGKEYELVITTYSGLCRYRVGDILHVTGFHNAAPQFRF 420

Query: 421 VRRKNVLLSIDSDKTDEAELQKAIDNAALLLRGFNTSVVEYTSHADTKTIPGHYVIYWEL 480
           VRRKNVLLSIDSDKTDEAELQKAIDNAALLLRGFNTSVVEYTS+ADTKTIPGHYVIYWEL
Sbjct: 421 VRRKNVLLSIDSDKTDEAELQKAIDNAALLLRGFNTSVVEYTSYADTKTIPGHYVIYWEL 480

Query: 481 LVKDEETGNFPPGEILNQCCLAIEESLNSVYRQGRVADNSIGPLEIRVVRNGTFEELMDY 540
           LVKDEETGNFPPGEILNQCCLAIEESLNSVYRQGRVADNSIGPLEIRVVRNGTFEELMDY
Sbjct: 481 LVKDEETGNFPPGEILNQCCLAIEESLNSVYRQGRVADNSIGPLEIRVVRNGTFEELMDY 540

Query: 541 AISRGASINQYKAPRCVNFTPIIELLDSRVTSVHFSPSKPHWTPERRG 588
           AISRGASINQYKAPRCVNFTPIIELLDSRVTSVHFSPSKPHWTPERRG
Sbjct: 541 AISRGASINQYKAPRCVNFTPIIELLDSRVTSVHFSPSKPHWTPERRG 588

BLAST of Chy2G031400 vs. NCBI nr
Match: TYK12849.1 (putative indole-3-acetic acid-amido synthetase GH3.1 [Cucumis melo var. makuwa])

HSP 1 Score: 1145 bits (2961), Expect = 0.0
Identity = 571/588 (97.11%), Postives = 575/588 (97.79%), Query Frame = 0

Query: 1   MAVSSSVSHAKALQFIEEITTNADSVQQKVLAEILNRNSDTEYLKRFRLSRTASRDEFKS 60
           MA SSSVSHAKALQFIEEITTNADSVQQKVLAEILNRNSDTEYLKRFRLSR A RDEFKS
Sbjct: 1   MAASSSVSHAKALQFIEEITTNADSVQQKVLAEILNRNSDTEYLKRFRLSRAAGRDEFKS 60

Query: 61  NVRVVTYEDLQLDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKEEMERRQL 120
           NVRVVTYEDLQ DIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKEEMERRQL
Sbjct: 61  NVRVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKEEMERRQL 120

Query: 121 LYSLLMPIMNKYVPGLDKGKGLYFLFVKAETKTPGGLLARPVLTSYYKSDIFKTRPYDPF 180
           LYSLLMPIMNKYVPGLDKGKGLYFLFVKAETKTPGGL ARPVLTSYYKSDIF TRPYDPF
Sbjct: 121 LYSLLMPIMNKYVPGLDKGKGLYFLFVKAETKTPGGLPARPVLTSYYKSDIFMTRPYDPF 180

Query: 181 NDYSSPNEAILCANSFQSMYTQMLCGLLMREQVLRVGAVFASGLLRAIHFLQHNWKQLAH 240
           NDY+SPNEAILCANSFQSMYTQMLCGLLMR+QVLRVGAVFASGLLRAIHFLQ NWKQLAH
Sbjct: 181 NDYTSPNEAILCANSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAIHFLQLNWKQLAH 240

Query: 241 DISTATLNPKITDSCLRECVVSKYLTNPNPELAEFISKECSTEEWEGIITRIWPNTKYLD 300
           DISTATLNPKITDSCLREC+VSKYLTNPN ELAE ISKECSTEEWEGIITRIWPNTKYLD
Sbjct: 241 DISTATLNPKITDSCLRECIVSKYLTNPNSELAELISKECSTEEWEGIITRIWPNTKYLD 300

Query: 301 VIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMCYCE 360
           VIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMCY E
Sbjct: 301 VIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMCYFE 360

Query: 361 FIPLDNDMSSPPTQLVDLADVEVGKEYELVITTYSGLCRYRVGDILHVTGFHNAAPQFRF 420
           FIPLDNDMSSPPTQLVDLADVEVGKEYELVITTYSGLCRYRVGDILHVTGFHNAAPQFRF
Sbjct: 361 FIPLDNDMSSPPTQLVDLADVEVGKEYELVITTYSGLCRYRVGDILHVTGFHNAAPQFRF 420

Query: 421 VRRKNVLLSIDSDKTDEAELQKAIDNAALLLRGFNTSVVEYTSHADTKTIPGHYVIYWEL 480
           VRRKNVLLSIDSDKTDEAELQKAIDNAALLLRGFNTSVVEYTS+ADTKTIPGHYVIYWEL
Sbjct: 421 VRRKNVLLSIDSDKTDEAELQKAIDNAALLLRGFNTSVVEYTSYADTKTIPGHYVIYWEL 480

Query: 481 LVKDEETGNFPPGEILNQCCLAIEESLNSVYRQGRVADNSIGPLEIRVVRNGTFEELMDY 540
           LVKDEETGNFPPGEILNQ CLAIEESLNSVYRQGRVADNSIGPLEIRVVR GTFEELMDY
Sbjct: 481 LVKDEETGNFPPGEILNQSCLAIEESLNSVYRQGRVADNSIGPLEIRVVRKGTFEELMDY 540

Query: 541 AISRGASINQYKAPRCVNFTPIIELLDSRVTSVHFSPSKPHWTPERRG 588
           AISRGASINQYKAPRCVNFTPIIELLDSRVTSVHFSPSKPHWTPE RG
Sbjct: 541 AISRGASINQYKAPRCVNFTPIIELLDSRVTSVHFSPSKPHWTPEPRG 588

BLAST of Chy2G031400 vs. NCBI nr
Match: XP_008440334.1 (PREDICTED: probable indole-3-acetic acid-amido synthetase GH3.1 [Cucumis melo])

HSP 1 Score: 1142 bits (2955), Expect = 0.0
Identity = 570/588 (96.94%), Postives = 575/588 (97.79%), Query Frame = 0

Query: 1   MAVSSSVSHAKALQFIEEITTNADSVQQKVLAEILNRNSDTEYLKRFRLSRTASRDEFKS 60
           MA SSSVSHAKALQFIEEITTNADSVQQKVLAEILNRNSDTEYLKRFRLSR+A RDEFKS
Sbjct: 1   MAASSSVSHAKALQFIEEITTNADSVQQKVLAEILNRNSDTEYLKRFRLSRSAGRDEFKS 60

Query: 61  NVRVVTYEDLQLDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKEEMERRQL 120
           NVRVVTYEDLQ DIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIK EMERRQL
Sbjct: 61  NVRVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKGEMERRQL 120

Query: 121 LYSLLMPIMNKYVPGLDKGKGLYFLFVKAETKTPGGLLARPVLTSYYKSDIFKTRPYDPF 180
           LYSLLMPIMNKYVPGLDKGKGLYFLFVKAETKTPGGL ARPVLTSYYKSDIF TRPYDPF
Sbjct: 121 LYSLLMPIMNKYVPGLDKGKGLYFLFVKAETKTPGGLPARPVLTSYYKSDIFMTRPYDPF 180

Query: 181 NDYSSPNEAILCANSFQSMYTQMLCGLLMREQVLRVGAVFASGLLRAIHFLQHNWKQLAH 240
           NDY+SPNEAILCANSFQSMYTQMLCGLLMR+QVLRVGAVFASGLLRAIHFLQ NWKQLAH
Sbjct: 181 NDYTSPNEAILCANSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAIHFLQLNWKQLAH 240

Query: 241 DISTATLNPKITDSCLRECVVSKYLTNPNPELAEFISKECSTEEWEGIITRIWPNTKYLD 300
           DISTATLNPKITDSCLREC+VSKYLTNPN ELAE ISKECSTEEWEGIITRIWPNTKYLD
Sbjct: 241 DISTATLNPKITDSCLRECIVSKYLTNPNSELAELISKECSTEEWEGIITRIWPNTKYLD 300

Query: 301 VIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMCYCE 360
           VIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMCY E
Sbjct: 301 VIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMCYFE 360

Query: 361 FIPLDNDMSSPPTQLVDLADVEVGKEYELVITTYSGLCRYRVGDILHVTGFHNAAPQFRF 420
           FIPLDNDMSSPPTQLVDLADVEVGKEYELVITTYSGLCRYRVGDILHVTGFHNAAPQFRF
Sbjct: 361 FIPLDNDMSSPPTQLVDLADVEVGKEYELVITTYSGLCRYRVGDILHVTGFHNAAPQFRF 420

Query: 421 VRRKNVLLSIDSDKTDEAELQKAIDNAALLLRGFNTSVVEYTSHADTKTIPGHYVIYWEL 480
           VRRKNVLLSIDSDKTDEAELQKAIDNAALLLRGFNTSVVEYTS+ADTKTIPGHYVIYWEL
Sbjct: 421 VRRKNVLLSIDSDKTDEAELQKAIDNAALLLRGFNTSVVEYTSYADTKTIPGHYVIYWEL 480

Query: 481 LVKDEETGNFPPGEILNQCCLAIEESLNSVYRQGRVADNSIGPLEIRVVRNGTFEELMDY 540
           LVKDEETGNFPPGEILNQ CLAIEESLNSVYRQGRVADNSIGPLEIRVVR GTFEELMDY
Sbjct: 481 LVKDEETGNFPPGEILNQSCLAIEESLNSVYRQGRVADNSIGPLEIRVVRKGTFEELMDY 540

Query: 541 AISRGASINQYKAPRCVNFTPIIELLDSRVTSVHFSPSKPHWTPERRG 588
           AISRGASINQYKAPRCVNFTPIIELLDSRVTSVHFSPSKPHWTPE RG
Sbjct: 541 AISRGASINQYKAPRCVNFTPIIELLDSRVTSVHFSPSKPHWTPEPRG 588

BLAST of Chy2G031400 vs. NCBI nr
Match: XP_038881399.1 (probable indole-3-acetic acid-amido synthetase GH3.1 [Benincasa hispida])

HSP 1 Score: 1086 bits (2809), Expect = 0.0
Identity = 544/587 (92.67%), Postives = 564/587 (96.08%), Query Frame = 0

Query: 1   MAVSSSVSHAKALQFIEEITTNADSVQQKVLAEILNRNSDTEYLKRFRLSRTASRDEFKS 60
           MAV SS+SHAKAL+FIEEITT+ADSVQQ+VLAEILN+N+DTEYL RFRLSRTA RDEFKS
Sbjct: 1   MAVVSSLSHAKALRFIEEITTDADSVQQRVLAEILNQNADTEYLNRFRLSRTAGRDEFKS 60

Query: 61  NVRVVTYEDLQLDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKEEMERRQL 120
            V VVTYEDLQ DIQRIANGDRSPIFSS PISEFLTSSGTSAGERKLMPTIKEEMERRQL
Sbjct: 61  KVPVVTYEDLQPDIQRIANGDRSPIFSSRPISEFLTSSGTSAGERKLMPTIKEEMERRQL 120

Query: 121 LYSLLMPIMNKYVPGLDKGKGLYFLFVKAETKTPGGLLARPVLTSYYKSDIFKTRPYDPF 180
           LYSLLMPIMNK+VPGLDKGKGLYFLFVKAETKTPGGLLARPVLTSYYKSDIFKTRPYDPF
Sbjct: 121 LYSLLMPIMNKFVPGLDKGKGLYFLFVKAETKTPGGLLARPVLTSYYKSDIFKTRPYDPF 180

Query: 181 NDYSSPNEAILCANSFQSMYTQMLCGLLMREQVLRVGAVFASGLLRAIHFLQHNWKQLAH 240
           NDY+SPN+AILC NSFQSMYTQMLCGLLMR QVLRVGAVFASGLLRAIHFLQ NWKQLAH
Sbjct: 181 NDYTSPNDAILCPNSFQSMYTQMLCGLLMRGQVLRVGAVFASGLLRAIHFLQLNWKQLAH 240

Query: 241 DISTATLNPKITDSCLRECVVSKYLTNPNPELAEFISKECSTEEWEGIITRIWPNTKYLD 300
           D++T TLNPKITDSCLREC+ SKYLTNPNPELAEFISKECSTEEWEGIITRIWPNTKYLD
Sbjct: 241 DVATGTLNPKITDSCLRECITSKYLTNPNPELAEFISKECSTEEWEGIITRIWPNTKYLD 300

Query: 301 VIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMCYCE 360
           VIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPS+VTYTIMPNM Y E
Sbjct: 301 VIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSEVTYTIMPNMGYFE 360

Query: 361 FIPLDNDMSSPPTQLVDLADVEVGKEYELVITTYSGLCRYRVGDILHVTGFHNAAPQFRF 420
           F+P  +DMSS PT+LVDLADVEVGKEYELVITTYSGLCRYRVGDILHVTGFHNAAPQFRF
Sbjct: 361 FLP--HDMSSSPTRLVDLADVEVGKEYELVITTYSGLCRYRVGDILHVTGFHNAAPQFRF 420

Query: 421 VRRKNVLLSIDSDKTDEAELQKAIDNAALLLRGFNTSVVEYTSHADTKTIPGHYVIYWEL 480
           VRRKNVLLSIDSDKTDEAELQKAIDNAALLL+GFNTSVVEYTS+ADTKTIPGHYVIYWEL
Sbjct: 421 VRRKNVLLSIDSDKTDEAELQKAIDNAALLLKGFNTSVVEYTSYADTKTIPGHYVIYWEL 480

Query: 481 LVKDEETGNFPPGEILNQCCLAIEESLNSVYRQGRVADNSIGPLEIRVVRNGTFEELMDY 540
           LVKDE T NFP  EILN CCLAIEESLNSVYRQ RVADNSIGPLEIRVV+NGTFEELMDY
Sbjct: 481 LVKDEVT-NFPSREILNLCCLAIEESLNSVYRQCRVADNSIGPLEIRVVKNGTFEELMDY 540

Query: 541 AISRGASINQYKAPRCVNFTPIIELLDSRVTSVHFSPSKPHWTPERR 587
           AISRGASINQYKAPRCVNFTPI+ELLDSRVTSVHFSPSKPHWTPERR
Sbjct: 541 AISRGASINQYKAPRCVNFTPILELLDSRVTSVHFSPSKPHWTPERR 584

BLAST of Chy2G031400 vs. NCBI nr
Match: XP_008440335.1 (PREDICTED: probable indole-3-acetic acid-amido synthetase GH3.1 [Cucumis melo] >TYK12848.1 putative indole-3-acetic acid-amido synthetase GH3.1 [Cucumis melo var. makuwa])

HSP 1 Score: 1035 bits (2676), Expect = 0.0
Identity = 518/586 (88.40%), Postives = 542/586 (92.49%), Query Frame = 0

Query: 10  AKALQFIEEITTNADSVQQKVLAEILNRNSDTEYLKRFRLSRTASRDEFKSNVRVVTYED 69
           AKAL+FIEE TTNAD VQQ+VLAEIL +N+ TEY+ RFRL+    RD FKS   VVTYED
Sbjct: 22  AKALRFIEETTTNADLVQQRVLAEILTQNAHTEYVNRFRLNGATDRDTFKSKFPVVTYED 81

Query: 70  LQLDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKEEMERRQLLYSLLMPIM 129
           LQ DIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKEEMERRQLLYSLLMPIM
Sbjct: 82  LQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKEEMERRQLLYSLLMPIM 141

Query: 130 NKYVPGLDKGKGLYFLFVKAETKTPGGLLARPVLTSYYKSDIFKTRPYDPFNDYSSPNEA 189
           N YVPGLDKGKGLYFLFVKAETKTPGGL+ARPVLTSYYKSDIFKTRPYDPFNDY+SPNEA
Sbjct: 142 NLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDIFKTRPYDPFNDYTSPNEA 201

Query: 190 ILCANSFQSMYTQMLCGLLMREQVLRVGAVFASGLLRAIHFLQHNWKQLAHDISTATLNP 249
           +LCA+SFQSMYTQMLCGLLMR+QVLRVGAVFASGLLRAI FLQ NWKQLAHDIST TLNP
Sbjct: 202 VLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAIRFLQLNWKQLAHDISTGTLNP 261

Query: 250 KITDSCLRECVVSKYLTNPNPELAEFISKECSTEEWEGIITRIWPNTKYLDVIVTGAMAQ 309
           KITD+CLREC+ SKYLTNPNPELAEFIS EC TEEWEGIITRIWPNTKYLDVIVTGAMAQ
Sbjct: 262 KITDTCLRECITSKYLTNPNPELAEFISNECYTEEWEGIITRIWPNTKYLDVIVTGAMAQ 321

Query: 310 YIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMCYCEFIPLDNDM- 369
           YIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNM Y EF+P D+   
Sbjct: 322 YIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMGYFEFLPHDSSSR 381

Query: 370 ------SSPPTQLVDLADVEVGKEYELVITTYSGLCRYRVGDILHVTGFHNAAPQFRFVR 429
                  SPP +LVDLADVEVGKEYELVITTY+GLCRYRVGD+L VTGFHNAAPQFRFVR
Sbjct: 382 ASALSRDSPP-RLVDLADVEVGKEYELVITTYAGLCRYRVGDVLEVTGFHNAAPQFRFVR 441

Query: 430 RKNVLLSIDSDKTDEAELQKAIDNAALLLRGFNTSVVEYTSHADTKTIPGHYVIYWELLV 489
           RKNVLLSIDSDKTDEAELQKA++NA+ LLR FNT+VVEYTS+ADTKTIPGHYVIYWELLV
Sbjct: 442 RKNVLLSIDSDKTDEAELQKAVENASSLLRDFNTTVVEYTSYADTKTIPGHYVIYWELLV 501

Query: 490 KDEETGN-FPPGEILNQCCLAIEESLNSVYRQGRVADNSIGPLEIRVVRNGTFEELMDYA 549
           KD ET N  P  EILNQCCLAIEESLNSVYRQGRVADNSIGPLEIRVVRNGTFEELMDYA
Sbjct: 502 KDNETMNSHPTDEILNQCCLAIEESLNSVYRQGRVADNSIGPLEIRVVRNGTFEELMDYA 561

Query: 550 ISRGASINQYKAPRCVNFTPIIELLDSRVTSVHFSPSKPHWTPERR 587
           ISRGASINQYKAPRCVNFTPI+ELLDSRVTSVHFSPSKPHWTPERR
Sbjct: 562 ISRGASINQYKAPRCVNFTPIVELLDSRVTSVHFSPSKPHWTPERR 606

BLAST of Chy2G031400 vs. TAIR 10
Match: AT2G14960.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 885.9 bits (2288), Expect = 1.7e-257
Identity = 440/598 (73.58%), Postives = 501/598 (83.78%), Query Frame = 0

Query: 1   MAVSSSVS----------HAKALQFIEEITTNADSVQQKVLAEILNRNSDTEYLKRFRLS 60
           MAV S++S           AKAL+FIEE+T NAD+VQ+ +LAEIL RN+DTEYL+RF L 
Sbjct: 1   MAVDSNLSSPLGPPACEKDAKALRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLC 60

Query: 61  RTASRDEFKSNVRVVTYEDLQLDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPT 120
               RD FK+ + V+TYEDLQ +IQRIA+GDRSPI S+HPISEFLTSSGTSAGERKLMPT
Sbjct: 61  GATDRDTFKTKIPVITYEDLQPEIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEEMERRQLLYSLLMPIMNKYVPGLDKGKGLYFLFVKAETKTPGGLLARPVLTSYYKSD 180
           IKEE++RRQLLYSLLMP+MN YVPGLDKGKG+YFLFVK+ETKTPGGL ARPVLTSYYKS+
Sbjct: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSE 180

Query: 181 IFKTRPYDPFNDYSSPNEAILCANSFQSMYTQMLCGLLMREQVLRVGAVFASGLLRAIHF 240
            F++RPYDP+N Y+SPNEAILC +SFQSMYTQMLCGLL R  VLRVGAVFASGLLRAI F
Sbjct: 181 HFRSRPYDPYNVYTSPNEAILCPDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRF 240

Query: 241 LQHNWKQLAHDISTATLNPKITDSCLRECVVSKYLTNPNPELAEFISKECSTEEWEGIIT 300
           LQ +W + AHDI    L+ +ITD  +R+C+    +  P+P LAEFI +EC ++ WE IIT
Sbjct: 241 LQLHWSRFAHDIELGCLDSEITDPSIRQCMSG--ILKPDPVLAEFIRRECKSDNWEKIIT 300

Query: 301 RIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTY 360
           RIWPNTKYLDVIVTGAMAQYIPTLE+YSGGLPMACTMYASSECYFG+NLNPM KPS+V+Y
Sbjct: 301 RIWPNTKYLDVIVTGAMAQYIPTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSY 360

Query: 361 TIMPNMCYCEFIPLDNDMSSPPTQLVDLADVEVGKEYELVITTYSGLCRYRVGDILHVTG 420
           TIMPNM Y EFIPL        T+ V+L DV +GKEYELV+TTY+GLCRYRVGDIL VTG
Sbjct: 361 TIMPNMAYFEFIPLGG------TKAVELVDVNIGKEYELVVTTYAGLCRYRVGDILRVTG 420

Query: 421 FHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAIDNAALLL-RGFNTSVVEYTSHADTKT 480
           FHN+APQF FVRRKNVLLSIDSDKTDE+ELQKA++NA+ +L     + V EYTS+ADT T
Sbjct: 421 FHNSAPQFHFVRRKNVLLSIDSDKTDESELQKAVENASSILHEECGSRVAEYTSYADTST 480

Query: 481 IPGHYVIYWELLVKDEETGNFPPGEILNQCCLAIEESLNSVYRQGRVADNSIGPLEIRVV 540
           IPGHYV+YWELLV+D      P  E L +CCL +EESLNSVYRQ RVADNS+GPLEIRVV
Sbjct: 481 IPGHYVLYWELLVRD--GARQPSHETLTRCCLGMEESLNSVYRQSRVADNSVGPLEIRVV 540

Query: 541 RNGTFEELMDYAISRGASINQYKAPRCVNFTPIIELLDSRVTSVHFSPSKPHWTPERR 588
           RNGTFEELMDYAISRGASINQYK PRCVNFTPI+ELLDSRV S HFSPS PHWTPERR
Sbjct: 541 RNGTFEELMDYAISRGASINQYKVPRCVNFTPIVELLDSRVVSAHFSPSLPHWTPERR 588

BLAST of Chy2G031400 vs. TAIR 10
Match: AT4G37390.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 879.4 bits (2271), Expect = 1.6e-255
Identity = 437/593 (73.69%), Postives = 502/593 (84.65%), Query Frame = 0

Query: 1   MAVSSSVSHAKALQFIEEITTNADSVQQKVLAEILNRNSDTEYLKRFRLSRTASRDEFKS 60
           ++ ++S    KAL+FIEE+T N DSVQ+KVL EIL RNS+TEYLKRF L     R  FKS
Sbjct: 12  VSATTSEKDVKALKFIEEMTRNPDSVQEKVLGEILTRNSNTEYLKRFDLDGVVDRKTFKS 71

Query: 61  NVRVVTYEDLQLDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKEEMERRQL 120
            V VVTYEDL+ +IQRI+NGD SPI SSHPI+EFLTSSGTSAGERKLMPTI+E+++RRQL
Sbjct: 72  KVPVVTYEDLKPEIQRISNGDCSPILSSHPITEFLTSSGTSAGERKLMPTIEEDLDRRQL 131

Query: 121 LYSLLMPIMNKYVPGLDKGKGLYFLFVKAETKTPGGLLARPVLTSYYKSDIFKTRPYDPF 180
           LYSLLMP+MN YVPGLDKGKGLYFLFVK+E+KT GGL ARPVLTSYYKSD FK RPYDP+
Sbjct: 132 LYSLLMPVMNLYVPGLDKGKGLYFLFVKSESKTSGGLPARPVLTSYYKSDHFKRRPYDPY 191

Query: 181 NDYSSPNEAILCANSFQSMYTQMLCGLLMREQVLRVGAVFASGLLRAIHFLQHNWKQLAH 240
           N Y+SPNEAILC++S QSMY QMLCGLLMR +VLR+GAVFASGLLRAI FLQ+NWK+LA 
Sbjct: 192 NVYTSPNEAILCSDSSQSMYAQMLCGLLMRHEVLRLGAVFASGLLRAISFLQNNWKELAR 251

Query: 241 DISTATLNPKITDSCLRECVVSKYLTNPNPELAEFISKECSTEEWEGIITRIWPNTKYLD 300
           DIST TL+ +I D  ++   +SK LT P+ ELAEF+   CS E WEGIIT+IWPNTKYLD
Sbjct: 252 DISTGTLSSRIFDPAIKN-RMSKILTKPDQELAEFLVGVCSQENWEGIITKIWPNTKYLD 311

Query: 301 VIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMCYCE 360
           VIVTGAMAQYIPTLE+YSGGLPMACTMYASSE YFG+NL PMCKPS+V+YTIMPNM Y E
Sbjct: 312 VIVTGAMAQYIPTLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIMPNMAYFE 371

Query: 361 FIPLDND------MSSPPTQLVDLADVEVGKEYELVITTYSGLCRYRVGDILHVTGFHNA 420
           F+P ++D       S   T LV+LA+VEVGKEYELVITTY+GL RYRVGDIL VTGFHN+
Sbjct: 372 FLPHNHDGDGAAEASLDETSLVELANVEVGKEYELVITTYAGLYRYRVGDILRVTGFHNS 431

Query: 421 APQFRFVRRKNVLLSIDSDKTDEAELQKAIDNAALLLRGFNTSVVEYTSHADTKTIPGHY 480
           APQF+F+RRKNVLLS++SDKTDEAELQKA++NA+ L     T V+EYTS+A+TKTIPGHY
Sbjct: 432 APQFKFIRRKNVLLSVESDKTDEAELQKAVENASRLFAEQGTRVIEYTSYAETKTIPGHY 491

Query: 481 VIYWELLVKDEETGNFPPGEILNQCCLAIEESLNSVYRQGRVADNSIGPLEIRVVRNGTF 540
           VIYWELL +D+        E++ +CCL +EESLNSVYRQ RVAD SIGPLEIRVVRNGTF
Sbjct: 492 VIYWELLGRDQSNA-LMSEEVMAKCCLEMEESLNSVYRQSRVADKSIGPLEIRVVRNGTF 551

Query: 541 EELMDYAISRGASINQYKAPRCVNFTPIIELLDSRVTSVHFSPSKPHWTPERR 588
           EELMDYAISRGASINQYK PRCV+FTPI+ELLDSRV S HFSPS PHW+PERR
Sbjct: 552 EELMDYAISRGASINQYKVPRCVSFTPIMELLDSRVVSAHFSPSLPHWSPERR 602

BLAST of Chy2G031400 vs. TAIR 10
Match: AT2G23170.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 869.0 bits (2244), Expect = 2.2e-252
Identity = 435/588 (73.98%), Postives = 501/588 (85.20%), Query Frame = 0

Query: 1   MAVSSSVSHAKALQFIEEITTNADSVQQKVLAEILNRNSDTEYLKRFRLSRTASRDEFKS 60
           M  S S    KAL+FIEE+T N D VQ+KV+ EIL+RNSDTEYLKRF L     R  FK+
Sbjct: 11  MMHSPSTKDVKALRFIEEMTRNVDFVQKKVIREILSRNSDTEYLKRFGLKGFTDRKTFKT 70

Query: 61  NVRVVTYEDLQLDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKEEMERRQL 120
            V VV Y+DL+ +IQRIANGDRS I SS+PI+EFLTSSGTSAGERKLMPTI E+M+RRQL
Sbjct: 71  KVPVVIYDDLKPEIQRIANGDRSMILSSYPITEFLTSSGTSAGERKLMPTIDEDMDRRQL 130

Query: 121 LYSLLMPIMNKYVPGLDKGKGLYFLFVKAETKTPGGLLARPVLTSYYKSDIFKTRPYDPF 180
           LYSLLMP+MN YVPGLDKGK LYFLFVK E+KTPGGL ARPVLTSYYKS+ FK RP DP+
Sbjct: 131 LYSLLMPVMNLYVPGLDKGKALYFLFVKTESKTPGGLPARPVLTSYYKSEQFKRRPNDPY 190

Query: 181 NDYSSPNEAILCANSFQSMYTQMLCGLLMREQVLRVGAVFASGLLRAIHFLQHNWKQLAH 240
           N Y+SPNEAILC +S QSMYTQMLCGLLMR +VLR+GAVFASGLLRAI FLQ NWK+LA 
Sbjct: 191 NVYTSPNEAILCPDSSQSMYTQMLCGLLMRHEVLRLGAVFASGLLRAIGFLQTNWKELAD 250

Query: 241 DISTATLNPKITDSCLRECVVSKYLTNPNPELAEFISKECSTE-EWEGIITRIWPNTKYL 300
           DIST TL+ +I+D  ++E  +SK LT P+ ELA+FI+  C  +  WEGIIT+IWPNTKYL
Sbjct: 251 DISTGTLSSRISDPAIKES-MSKILTKPDQELADFITSVCGQDNSWEGIITKIWPNTKYL 310

Query: 301 DVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMCYC 360
           DVIVTGAMAQYIP LE+YSGGLPMACTMYASSE YFG+NL PMCKPS+V+YTIMPNM Y 
Sbjct: 311 DVIVTGAMAQYIPMLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIMPNMAYF 370

Query: 361 EFIPLDNDMSSPPTQLVDLADVEVGKEYELVITTYSGLCRYRVGDILHVTGFHNAAPQFR 420
           EF+P  +++ +  ++LV+LADVEVGKEYELVITTY+GL RYRVGDIL VTGF+N+APQF+
Sbjct: 371 EFLP-HHEVPTEKSELVELADVEVGKEYELVITTYAGLNRYRVGDILQVTGFYNSAPQFK 430

Query: 421 FVRRKNVLLSIDSDKTDEAELQKAIDNAALLLRGFNTSVVEYTSHADTKTIPGHYVIYWE 480
           FVRRKNVLLSI+SDKTDEAELQ A++NA+LLL    T V+EYTS+A+TKTIPGHYVIYWE
Sbjct: 431 FVRRKNVLLSIESDKTDEAELQSAVENASLLLGEQGTRVIEYTSYAETKTIPGHYVIYWE 490

Query: 481 LLVKDEETGNFPPGEILNQCCLAIEESLNSVYRQGRVADNSIGPLEIRVVRNGTFEELMD 540
           LLVKD+   N P  E++ +CCL +EESLNSVYRQ RVAD SIGPLEIRVV+NGTFEELMD
Sbjct: 491 LLVKDQT--NPPNDEVMARCCLEMEESLNSVYRQSRVADKSIGPLEIRVVKNGTFEELMD 550

Query: 541 YAISRGASINQYKAPRCVNFTPIIELLDSRVTSVHFSPSKPHWTPERR 588
           YAISRGASINQYK PRCV+FTPI+ELLDSRV S HFSP+ PHW+PERR
Sbjct: 551 YAISRGASINQYKVPRCVSFTPIMELLDSRVVSTHFSPALPHWSPERR 594

BLAST of Chy2G031400 vs. TAIR 10
Match: AT1G59500.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 833.2 bits (2151), Expect = 1.3e-241
Identity = 416/586 (70.99%), Postives = 486/586 (82.94%), Query Frame = 0

Query: 4   SSSVSHAKALQFIEEITTNADSVQQKVLAEILNRNSDTEYLKRFRLSRTASRDEFKSNVR 63
           ++S +  KAL+FIEEIT N DSVQ+KVL EIL+RNS+TEYLKRF L+    R  FKS V 
Sbjct: 15  TTSETEVKALKFIEEITRNPDSVQEKVLGEILSRNSNTEYLKRFDLNGAVDRKSFKSKVP 74

Query: 64  VVTYEDLQLDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKEEMERRQLLYS 123
           VV YEDL+ DIQRI+NGDRSPI SSHPI+EFLTSSGTSAGERKLMPTI+E++ RRQLL +
Sbjct: 75  VVIYEDLKTDIQRISNGDRSPILSSHPITEFLTSSGTSAGERKLMPTIEEDINRRQLLGN 134

Query: 124 LLMPIMNKYVPGLDKGKGLYFLFVKAETKTPGGLLARPVLTSYYKSDIFKTRPYDPFNDY 183
           LLMP+MN YVPGLDKGKGLYFLFVK+E+ T GGL ARP LTSYYKSD F+T   D  + Y
Sbjct: 135 LLMPVMNLYVPGLDKGKGLYFLFVKSESTTSGGLPARPALTSYYKSDYFRTSDSD--SVY 194

Query: 184 SSPNEAILCANSFQSMYTQMLCGLLMREQVLRVGAVFASGLLRAIHFLQHNWKQLAHDIS 243
           +SP EAILC +S QSMYTQMLCGLLMR +V R+GAVF SGLLRAI FLQ+NWK+L+ DIS
Sbjct: 195 TSPKEAILCCDSSQSMYTQMLCGLLMRHEVNRLGAVFPSGLLRAISFLQNNWKELSQDIS 254

Query: 244 TATLNPKITDSCLRECVVSKYLTNPNPELAEFISKECSTEEWEGIITRIWPNTKYLDVIV 303
           T TL+ KI D  ++   +S  L  P+ ELAEF+   CS E WEGIIT+IWPNTKYLDVIV
Sbjct: 255 TGTLSSKIFDHAIK-TRMSNILNKPDQELAEFLIGVCSQENWEGIITKIWPNTKYLDVIV 314

Query: 304 TGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMCYCEFIP 363
           TGAMA+YIP LE+YSGGLPMA  +YASSE YFG+NLNPMCKPS+V+YTI PNM Y EF+P
Sbjct: 315 TGAMAEYIPMLEYYSGGLPMASMIYASSESYFGINLNPMCKPSEVSYTIFPNMAYFEFLP 374

Query: 364 --LDNDMSSPPTQLVDLADVEVGKEYELVITTYSGLCRYRVGDILHVTGFHNAAPQFRFV 423
              D D     T LV+LADVEVGKEYELVITTY+GL RYRVGDIL VTGFHN+APQF+F+
Sbjct: 375 HNHDGDGGVEATSLVELADVEVGKEYELVITTYAGLYRYRVGDILRVTGFHNSAPQFKFI 434

Query: 424 RRKNVLLSIDSDKTDEAELQKAIDNAALLLRGFNTSVVEYTSHADTKTIPGHYVIYWELL 483
           RR+NVLLSI+SDKTDEA+LQKA++NA+ LL    T V+EYTS+ADTKTIPGHYVIYWELL
Sbjct: 435 RRENVLLSIESDKTDEADLQKAVENASRLLAEQGTRVIEYTSYADTKTIPGHYVIYWELL 494

Query: 484 VKDEETGNFPPGEILNQCCLAIEESLNSVYRQGRVADNSIGPLEIRVVRNGTFEELMDYA 543
            +D+     P  E++ +CCL +EESLN+VYRQ RV+D SIGPLEIRVV+NGTFEELMD++
Sbjct: 495 SRDQSNA-LPSDEVMAKCCLEMEESLNAVYRQSRVSDKSIGPLEIRVVQNGTFEELMDFS 554

Query: 544 ISRGASINQYKAPRCVNFTPIIELLDSRVTSVHFSPSKPHWTPERR 588
           ISRG+SINQYK PRCV+ TPI++LLDSRV S HFSPS PHW+PERR
Sbjct: 555 ISRGSSINQYKVPRCVSLTPIMKLLDSRVVSAHFSPSLPHWSPERR 596

BLAST of Chy2G031400 vs. TAIR 10
Match: AT5G54510.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 777.7 bits (2007), Expect = 6.6e-225
Identity = 376/586 (64.16%), Postives = 465/586 (79.35%), Query Frame = 0

Query: 13  LQFIEEITTNADSVQQKVLAEILNRNSDTEYLKRFRLSRTASRDEFKSNVRVVTYEDLQL 72
           LQFIE++TTNAD VQ++VL EIL+RN+D EYLKR  L     R+ FK  + VVTYED+Q 
Sbjct: 25  LQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEGRTDRETFKHIMPVVTYEDIQP 84

Query: 73  DIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKEEMERRQLLYSLLMPIMNKY 132
           +I RIANGD+S +  S+PISEFLTSSGTS GERKLMPTI+EE++RR LLYSLLMP+M+++
Sbjct: 85  EINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSLLMPVMDQF 144

Query: 133 VPGLDKGKGLYFLFVKAETKTPGGLLARPVLTSYYKSDIFKTRPYDPFNDYSSPNEAILC 192
           VPGLDKGKG+YFLF+K+E+KTPGGL ARPVLTSYYKS  FK RPYDP+ +Y+SPN+ ILC
Sbjct: 145 VPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKNRPYDPYTNYTSPNQTILC 204

Query: 193 ANSFQSMYTQMLCGLLMREQVLRVGAVFASGLLRAIHFLQHNWKQLAHDISTATLNPKIT 252
           ++S+QSMY+QMLCGL   ++VLRVGAVFASG +RAI FL+ +W +LA DI T TL+ +IT
Sbjct: 205 SDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPELARDIRTGTLSSEIT 264

Query: 253 DSCLRECVVSKYLTNPNPELAEFISKECSTEEWEGIITRIWPNTKYLDVIVTGAMAQYIP 312
           DS +RE V    +  P+P+LA+F+  EC    W+GIITR+WPNTKY+DVIVTG M+QYIP
Sbjct: 265 DSSVREAVGE--ILKPDPKLADFVESECRKTSWQGIITRLWPNTKYVDVIVTGTMSQYIP 324

Query: 313 TLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMCYCEFIP------LDN 372
           TL++YS GLP+ CTMYASSECYFGVNL P+CKPS+V+YT++PNM Y EF+P      + +
Sbjct: 325 TLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPNMAYFEFLPVHRNSGVTS 384

Query: 373 DMSSPPT-------QLVDLADVEVGKEYELVITTYSGLCRYRVGDILHVTGFHNAAPQFR 432
            +S P         +LVDL DV++G+EYELV+TTY+GL RYRVGD+L V GF N APQF 
Sbjct: 385 SISLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLYRYRVGDVLSVAGFKNNAPQFS 444

Query: 433 FVRRKNVLLSIDSDKTDEAELQKAIDNAALLLRGFNTSVVEYTSHADTKTIPGHYVIYWE 492
           F+ RKNV+LSIDSDKTDE ELQ A+ NA   L  F+ S+ EYTS+ADT +IPGHYV++WE
Sbjct: 445 FICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPGHYVLFWE 504

Query: 493 LLVKDEETGNFP-PGEILNQCCLAIEESLNSVYRQGRVADNSIGPLEIRVVRNGTFEELM 552
           L +     GN P P  +   CCL IEESLNSVYRQGRV+D SIGPLEI++V +GTF++LM
Sbjct: 505 LCL----NGNTPIPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKMVESGTFDKLM 564

Query: 553 DYAISRGASINQYKAPRCVNFTPIIELLDSRVTSVHFSPSKPHWTP 585
           DYAIS GASINQYK PRCV F PIIELL+SRV   +FSP  P W+P
Sbjct: 565 DYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWSP 604

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O823332.4e-25673.58Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Arabidopsis thaliana OX=... [more]
O221903.1e-25173.98Indole-3-acetic acid-amido synthetase GH3.3 OS=Arabidopsis thaliana OX=3702 GN=G... [more]
A3BLS04.3e-24572.79Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. indica OX=399... [more]
Q0D4Z64.3e-24572.79Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. japonica OX=3... [more]
Q9LQ681.9e-24070.99Indole-3-acetic acid-amido synthetase GH3.4 OS=Arabidopsis thaliana OX=3702 GN=G... [more]
Match NameE-valueIdentityDescription
A0A0A0KLF80.0e+0098.47Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G492310 PE=3 SV=1[more]
A0A5D3CR820.0e+0097.11Putative indole-3-acetic acid-amido synthetase GH3.1 OS=Cucumis melo var. makuwa... [more]
A0A1S3B1M00.0e+0096.94probable indole-3-acetic acid-amido synthetase GH3.1 OS=Cucumis melo OX=3656 GN=... [more]
A0A5D3CNV82.6e-30188.21Putative indole-3-acetic acid-amido synthetase GH3.1 OS=Cucumis melo var. makuwa... [more]
A0A1S3B0G22.6e-30188.21probable indole-3-acetic acid-amido synthetase GH3.1 OS=Cucumis melo OX=3656 GN=... [more]
Match NameE-valueIdentityDescription
XP_004141994.10.098.47probable indole-3-acetic acid-amido synthetase GH3.1 [Cucumis sativus] >KGN48571... [more]
TYK12849.10.097.11putative indole-3-acetic acid-amido synthetase GH3.1 [Cucumis melo var. makuwa][more]
XP_008440334.10.096.94PREDICTED: probable indole-3-acetic acid-amido synthetase GH3.1 [Cucumis melo][more]
XP_038881399.10.092.67probable indole-3-acetic acid-amido synthetase GH3.1 [Benincasa hispida][more]
XP_008440335.10.088.40PREDICTED: probable indole-3-acetic acid-amido synthetase GH3.1 [Cucumis melo] >... [more]
Match NameE-valueIdentityDescription
AT2G14960.11.7e-25773.58Auxin-responsive GH3 family protein [more]
AT4G37390.11.6e-25573.69Auxin-responsive GH3 family protein [more]
AT2G23170.12.2e-25273.98Auxin-responsive GH3 family protein [more]
AT1G59500.11.3e-24170.99Auxin-responsive GH3 family protein [more]
AT5G54510.16.6e-22564.16Auxin-responsive GH3 family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004993GH3 familyPFAMPF03321GH3coord: 16..559
e-value: 2.4E-199
score: 663.6
IPR004993GH3 familyPANTHERPTHR31901GH3 DOMAIN-CONTAINING PROTEINcoord: 5..587
NoneNo IPR availablePANTHERPTHR31901:SF7INDOLE-3-ACETIC ACID-AMIDO SYNTHETASE GH3.2-RELATEDcoord: 5..587

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy2G031400.1Chy2G031400.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005737 cytoplasm
molecular_function GO:0016881 acid-amino acid ligase activity