Chy2G031000 (gene) Cucumber (hystrix) v1

Overview


Name	Chy2G031000
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial
Location	chrH02: 5291374 .. 5294350 (-)
RNA-Seq Expression	Chy2G031000
Synteny	Chy2G031000

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTAACTCCTTGCAGCGAATCTGGAGCCACGGACGTGCTCTTCGGGGTACTGTGGCTCCTCTTTCCAGGTCATTCTCCACCGATTCCTTGGTTGAAGTCAAGCCTGGCGAGATCGGTATAGTCTCTGGAATTCCAGAGGAGCATCTTACCAGAAGGGTATAGCCCAGATATTTCTTTTTCTTTACTTTTTATCAACGAGTTTCCAATTGCTTACATTTTAGTTTAATTTCGAGGGATCGGGTTATTGGATGTTAGTGATGTTTTTTGGGTTTAGGTTTTTTTATACCCGTCTGCTTGGGTGTTCTTTCTTGTTTATAACTTGGACTTATTAGAATGGTTTGCAGTGGCTAAACCCCCTTAGGTGTTACTTTGTTACTGGTATGTTGAAATGTACTCGGTTCCCATGAGGAGATGGAAATGTCCGGCTGTTCATAACCAAAAATTGATTAAAATGAGGTTAAAGTATGTCGAGGAGCCCGAGTTTTCTCCTTATGTGCTACTATATATTGCTATTGGTTGCAATCTCAAGATCCTAATCAGTGGATAGGCAGAAATTCGTTCTTTTTTTTAAGTTGATTTAACATAGGGCTTGACCAATTACTATCTCATAGTTTCTGTAAGTCATTAGGACCAATTGTTCAATCATCTATTGTTTAGAGGTATGATAGGCAAATGTTGAGATTTTCTTTAAAAATATTGTTTTGAATCTCAATTATTTCAGGATTGATCTCTACATGTATTGAGGTTTAATCTTGTAATTCTAATACACTAATCATTGGTGCGGATTTTCCTGTCATGTTATTCGTTTTCTATACTGAACATCTAGATTCATTTGTCTTCCTTTTATGTAATTATGTCAGGTTGTAATTTACTCTCCTGCTAGAACTGCATCCCAACAAGGATCAGGCAAGGTTGGCAAGTGGAAAATCAATTTTGTGTCAACACAAAAGTATGAATTCTTATCTGATTGTTTTCTTTATTTTTTTCTGAGTTCGATCTTAAAGGGAAAAGAAATCAGAAAATTGTTGCTTCGTTATTTAACAGCAATTTTAATTCAGGTCTAGCTGATTTCTAGATGCTAGATTGAAATCTTGTGCAGAACATGTTATACTGGGCAAACTTCAAGTGCCCAAAAGTTACTGGTCCTTGTTCTTTTACTCTCTACAATTACAGTTTTTTGACCCTTATAATTTTTTTTCTTTTTGAAATCTTGTTTCTCTGATCAGGCACCTTTACTGATGGATTGGTTCCATTTTCTATTGCCTGTGTGTAATATTTAATTTGTTTTCATAAATGTGTGGTTTGTCGTTTAGAGAAGAAAAATATCATACAGAAGGGCAAAAGTTTTTTTATGCTTATCTGTCATGTTTGTAGGTGGGAGAACCCATTGATGGGGTGGACTTCTACTGGGGATCCCTATGCCAATGTAGGGGATTCTGCACTGTCTTTTGATAGCGAAGAAGCTGCAAAGGCATTTGCTGAGAAACATGGATGGGAATATGTGGTAATGCTTGCTTTAACCATTTAGAAAGATTTTTCTTCTTGTAATACCTTTATGTAGTTTGAGTTGTCATATCCTAGGTTAATTATTAATTATGTTGTTTTTCCACTGTCAGTTTCCTTCAAATAAGTAATACTTAGAGTCGGTAGTGTTGATTAATTGTGCACATACTCTAACATATTCTTGACAATGATCTTCGCATTTGCACAAGGTTGAAAGTGGAACAATGCATTCTGATTCTAAGTAAATAACTCGAAAGAGCCAACAAGACAGCCTTAAGGGCAGGGGTTGAGGAAATCCCCTCCCATAAAACTAATATGGAACAAATAAATAATACTAAAATGAAATTGACATTGGATATGGTTGTTTAGTATGTTTATTTAGAATAACAAATATGATTCATGGTTTCTGCTTTTGGGCTATACCATGGTGTTTGACTCTAAATGTAAGATCTTATTTTCGAAGATAGTGGAAATTTAGTCAGTTATATAATATCCCAGTATTAGTTTGAAGCTAGTCTTCATAAGCTTCGAGAGGAGAAATATGAGGGGGAGAAGTCAATTTTTCTTTCATTAGATTAAGAAAATTTATATTTCTTATTTTAGCATTCTCTTAACATTGCGTTACCATTCTGTTTTTATCCGGAGAGATTAAAAGTTTCTTTAATGTTCAGATTGAAGAAATACTTTTCTGGCCTACATATAATTATGAGACTACATTCATACCATCTCCAAAATTTGGGTTAACTTCTAATCTTTCTGTTGATTGTGTTTCTTGATTTTCTTTTTTTTTCCCTTCTTTTTGTAGGTTAAGAAGCGCCACACACCACTTTTGAAGGTTTGAGATTCTTTCCCATTATTTCAATTTCACTGAGCTTGTTAACTGTCTTTTTTAGTTCTGTGGGGGTTGGATACCCAAGTATAGAGTGATTCGTCCATTGAGCTTTCTGTCTTATTTACAAGCATCGATTATTTCCAAGGAATAAGCCCTTTTGTTAAATTTATACATTTGATCAATGCAACTTCTTTCATCATCTTTTAGAATTCTGCTGGCGTTTTTTGGTAATTATAAATCCTTTTGCAATATAGTCTTGTGAATAGTTTAGGGGAGATCAGTTTCAGTTTCCTCTTGGTACCTGTAGTTATTTCTATTTTCTAAATGACTTAAAGTTTCATTTGTTATTATTAGAGTACTTTATAGTCCTACTCCTAACTAACAGATCAGAGTTCTGTTTAAGATAGATGGAGGAGAAGGATGAAGTTCGGACAAATTGGTTTGAAAGTCGATCATGTTTAACACCCACTGATGAAGGAATTTTGATGCTGCTTGTAATTTTTATATCATCTAACTAACTCGAGCATCCAAAATAAAGAAACAGAAAAAACTTACTACTCCCTGCTTTGACTGTAGGCGAAGGCATACGCAGACAATTTCAAATGGAAGGGCCCTCCTGCTGCAGCCAAGGAGGCTTAG

mRNA sequence

ATGGCTAACTCCTTGCAGCGAATCTGGAGCCACGGACGTGCTCTTCGGGGTACTGTGGCTCCTCTTTCCAGGTCATTCTCCACCGATTCCTTGGTTGAAGTCAAGCCTGGCGAGATCGGTATAGTCTCTGGAATTCCAGAGGAGCATCTTACCAGAAGGGTTGTAATTTACTCTCCTGCTAGAACTGCATCCCAACAAGGATCAGGCAAGGTTGGCAAGTGGAAAATCAATTTTGTGTCAACACAAAAGTGGGAGAACCCATTGATGGGGTGGACTTCTACTGGGGATCCCTATGCCAATGTAGGGGATTCTGCACTGTCTTTTGATAGCGAAGAAGCTGCAAAGGCATTTGCTGAGAAACATGGATGGGAATATGTGGTTAAGAAGCGCCACACACCACTTTTGAAGGCGAAGGCATACGCAGACAATTTCAAATGGAAGGGCCCTCCTGCTGCAGCCAAGGAGGCTTAG

Coding sequence (CDS)

ATGGCTAACTCCTTGCAGCGAATCTGGAGCCACGGACGTGCTCTTCGGGGTACTGTGGCTCCTCTTTCCAGGTCATTCTCCACCGATTCCTTGGTTGAAGTCAAGCCTGGCGAGATCGGTATAGTCTCTGGAATTCCAGAGGAGCATCTTACCAGAAGGGTTGTAATTTACTCTCCTGCTAGAACTGCATCCCAACAAGGATCAGGCAAGGTTGGCAAGTGGAAAATCAATTTTGTGTCAACACAAAAGTGGGAGAACCCATTGATGGGGTGGACTTCTACTGGGGATCCCTATGCCAATGTAGGGGATTCTGCACTGTCTTTTGATAGCGAAGAAGCTGCAAAGGCATTTGCTGAGAAACATGGATGGGAATATGTGGTTAAGAAGCGCCACACACCACTTTTGAAGGCGAAGGCATACGCAGACAATTTCAAATGGAAGGGCCCTCCTGCTGCAGCCAAGGAGGCTTAG

Protein sequence

MANSLQRIWSHGRALRGTVAPLSRSFSTDSLVEVKPGEIGIVSGIPEEHLTRRVVIYSPARTASQQGSGKVGKWKINFVSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEKHGWEYVVKKRHTPLLKAKAYADNFKWKGPPAAAKEA*

Homology

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BLAST of Chy2G031000 vs. ExPASy Swiss-Prot
Match: Q9FJW4 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=FRO1 PE=1 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 1.5e-56
Identity = 103/138 (74.64%), Postives = 122/138 (88.41%), Query Frame = 0


Query: 15  LRGTVAPLSRSFSTDSLVE--VKPGEIGIVSGIPEEHLTRRVVIYSPARTASQQGSGKVG 74
           +  T+  ++R F+TD++VE   K GEIG VSGIPEEHL+R+V+IYSPARTA+Q GSGK+G
Sbjct: 13  IAATLRRVARPFATDAVVESDYKRGEIGKVSGIPEEHLSRKVIIYSPARTATQSGSGKLG 72

Query: 75  KWKINFVSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEKHGWEYVVKKRHT 134
           KWKINFVST KWENPLMGWTSTGDPYANVGDSAL+FDSEEAAK+FAE+HGW+Y VKK +T
Sbjct: 73  KWKINFVSTLKWENPLMGWTSTGDPYANVGDSALAFDSEEAAKSFAERHGWDYKVKKPNT 132

Query: 135 PLLKAKAYADNFKWKGPP 151
           PLLK K+Y+DNFKWKG P
Sbjct: 133 PLLKVKSYSDNFKWKGNP 150

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BLAST of Chy2G031000 vs. ExPASy Swiss-Prot
Match: Q5XIF3 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Rattus norvegicus OX=10116 GN=Ndufs4 PE=1 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 9.0e-25
Identity = 56/121 (46.28%), Postives = 77/121 (63.64%), Query Frame = 0


Query: 27  STDSLVEVKPGEIGIVSGIPEEHL-TRRVVIYSPARTASQQGSGKVGKWKINFVSTQKWE 86
           + D  ++V P     ++G+PEEH+ TR+V I+ PAR   Q G     KWK+ F + ++WE
Sbjct: 54  TVDEKLDVTP-----LTGVPEEHIKTRKVRIFVPARNNMQSGVNNTKKWKMEFDTRERWE 113

Query: 87  NPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEKHGWEYVVKKRHTPLLKAKAYADNFK 146
           NPLMGW ST DP +N+    L+F ++E A AFAEKHGW Y V+ R  P  K+K+Y  NF 
Sbjct: 114 NPLMGWASTADPLSNM---VLTFSAKEDAVAFAEKHGWSYDVEGRKVPKPKSKSYGANFS 166

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BLAST of Chy2G031000 vs. ExPASy Swiss-Prot
Match: Q02375 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Bos taurus OX=9913 GN=NDUFS4 PE=1 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 3.4e-24
Identity = 52/110 (47.27%), Postives = 73/110 (66.36%), Query Frame = 0


Query: 38  EIGIVSGIPEEHL-TRRVVIYSPARTASQQGSGKVGKWKINFVSTQKWENPLMGWTSTGD 97
           +I  ++G+PEEH+ TR+  I+ PAR   Q G     KWK+ F + ++WENPLMGW ST D
Sbjct: 60  DITTITGVPEEHIKTRKARIFVPARNNMQSGVNNTKKWKMEFDTRERWENPLMGWASTAD 119

Query: 98  PYANVGDSALSFDSEEAAKAFAEKHGWEYVVKKRHTPLLKAKAYADNFKW 147
           P +N+    L+F ++E A AFAEK+GW Y V++R  P  K+K+Y  NF W
Sbjct: 120 PLSNL---VLTFSTKEDAVAFAEKNGWSYDVEERKVPKPKSKSYGANFSW 166

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BLAST of Chy2G031000 vs. ExPASy Swiss-Prot
Match: Q0MQH0 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Gorilla gorilla gorilla OX=9595 GN=NDUFS4 PE=2 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 3.4e-24
Identity = 58/137 (42.34%), Postives = 89/137 (64.96%), Query Frame = 0


Query: 13  RALRGTVAPLSRSFSTDS-LVEV-KPGEIGIVSGIPEEHL-TRRVVIYSPARTASQQGSG 72
           R+LR +   L++  + D+ L+ V +  +I  ++G+PEEH+ TR+V I+ PAR   Q G  
Sbjct: 33  RSLRTSTWRLAQDQTQDTQLITVDEKLDITTLTGVPEEHIKTRKVRIFVPARNNMQSGVN 92

Query: 73  KVGKWKINFVSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEKHGWEYVVKK 132
              KWK+ F + ++WENPLMGW ST DP +N+    L+F ++E A +FAEK+GW Y +++
Sbjct: 93  NTKKWKMEFDTRERWENPLMGWASTADPLSNM---VLTFSTKEDAVSFAEKNGWSYDIEE 152

Query: 133 RHTPLLKAKAYADNFKW 147
           R  P  K+K+Y  NF W
Sbjct: 153 RKVPKPKSKSYGANFSW 166

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BLAST of Chy2G031000 vs. ExPASy Swiss-Prot
Match: O43181 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Homo sapiens OX=9606 GN=NDUFS4 PE=1 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 3.4e-24
Identity = 58/137 (42.34%), Postives = 89/137 (64.96%), Query Frame = 0


Query: 13  RALRGTVAPLSRSFSTDS-LVEV-KPGEIGIVSGIPEEHL-TRRVVIYSPARTASQQGSG 72
           R+LR +   L++  + D+ L+ V +  +I  ++G+PEEH+ TR+V I+ PAR   Q G  
Sbjct: 33  RSLRTSTWRLAQDQTQDTQLITVDEKLDITTLTGVPEEHIKTRKVRIFVPARNNMQSGVN 92

Query: 73  KVGKWKINFVSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEKHGWEYVVKK 132
              KWK+ F + ++WENPLMGW ST DP +N+    L+F ++E A +FAEK+GW Y +++
Sbjct: 93  NTKKWKMEFDTRERWENPLMGWASTADPLSNM---VLTFSTKEDAVSFAEKNGWSYDIEE 152

Query: 133 RHTPLLKAKAYADNFKW 147
           R  P  K+K+Y  NF W
Sbjct: 153 RKVPKPKSKSYGANFSW 166

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BLAST of Chy2G031000 vs. ExPASy TrEMBL
Match: A0A0A0KGI3 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Cucumis sativus OX=3659 GN=Csa_6G495720 PE=3 SV=1)

HSP 1 Score: 318.9 bits (816), Expect = 1.2e-83
Identity = 153/156 (98.08%), Postives = 156/156 (100.00%), Query Frame = 0


Query: 1   MANSLQRIWSHGRALRGTVAPLSRSFSTDSLVEVKPGEIGIVSGIPEEHLTRRVVIYSPA 60
           MANSLQRIWSHGRALRGT+APLSRSFSTDSLVE+KPGEIG+VSGIPEEHLTRRVVIYSPA
Sbjct: 1   MANSLQRIWSHGRALRGTLAPLSRSFSTDSLVEMKPGEIGVVSGIPEEHLTRRVVIYSPA 60

Query: 61  RTASQQGSGKVGKWKINFVSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120
           RTASQQGSGKVGKWKINFVSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK
Sbjct: 61  RTASQQGSGKVGKWKINFVSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120

Query: 121 HGWEYVVKKRHTPLLKAKAYADNFKWKGPPAAAKEA 157
           HGWEYVVKKRHTPLLKAKAYADNFKWKGPPAAAKEA
Sbjct: 121 HGWEYVVKKRHTPLLKAKAYADNFKWKGPPAAAKEA 156

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BLAST of Chy2G031000 vs. ExPASy TrEMBL
Match: A0A5D3CP24 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G003960 PE=3 SV=1)

HSP 1 Score: 315.1 bits (806), Expect = 1.7e-82
Identity = 152/156 (97.44%), Postives = 154/156 (98.72%), Query Frame = 0


Query: 1   MANSLQRIWSHGRALRGTVAPLSRSFSTDSLVEVKPGEIGIVSGIPEEHLTRRVVIYSPA 60
           MANSLQRIWSHGRALR TVAPLSRSFSTDSLV+VKPGEIG+VSGIPEEHLTRRVVIYSPA
Sbjct: 1   MANSLQRIWSHGRALRSTVAPLSRSFSTDSLVQVKPGEIGMVSGIPEEHLTRRVVIYSPA 60

Query: 61  RTASQQGSGKVGKWKINFVSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120
           RTASQQGSGKVGKWKINFVSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK
Sbjct: 61  RTASQQGSGKVGKWKINFVSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120

Query: 121 HGWEYVVKKRHTPLLKAKAYADNFKWKGPPAAAKEA 157
           HGWEYVVKKRHTPLLKAKAYADNFKWKGPPA AKEA
Sbjct: 121 HGWEYVVKKRHTPLLKAKAYADNFKWKGPPAEAKEA 156

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BLAST of Chy2G031000 vs. ExPASy TrEMBL
Match: A0A1S3B1N9 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103484861 PE=3 SV=1)

HSP 1 Score: 315.1 bits (806), Expect = 1.7e-82
Identity = 152/156 (97.44%), Postives = 154/156 (98.72%), Query Frame = 0


Query: 1   MANSLQRIWSHGRALRGTVAPLSRSFSTDSLVEVKPGEIGIVSGIPEEHLTRRVVIYSPA 60
           MANSLQRIWSHGRALR TVAPLSRSFSTDSLV+VKPGEIG+VSGIPEEHLTRRVVIYSPA
Sbjct: 1   MANSLQRIWSHGRALRSTVAPLSRSFSTDSLVQVKPGEIGMVSGIPEEHLTRRVVIYSPA 60

Query: 61  RTASQQGSGKVGKWKINFVSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120
           RTASQQGSGKVGKWKINFVSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK
Sbjct: 61  RTASQQGSGKVGKWKINFVSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120

Query: 121 HGWEYVVKKRHTPLLKAKAYADNFKWKGPPAAAKEA 157
           HGWEYVVKKRHTPLLKAKAYADNFKWKGPPA AKEA
Sbjct: 121 HGWEYVVKKRHTPLLKAKAYADNFKWKGPPAEAKEA 156

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BLAST of Chy2G031000 vs. ExPASy TrEMBL
Match: A0A6J1HE01 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111463327 PE=3 SV=1)

HSP 1 Score: 293.9 bits (751), Expect = 4.0e-76
Identity = 141/150 (94.00%), Postives = 145/150 (96.67%), Query Frame = 0


Query: 1   MANSLQRIWSHGRALRGTVAPLSRSFSTDSLVEVKPGEIGIVSGIPEEHLTRRVVIYSPA 60
           MANSLQRIW+ G ALRGT+APL RSFS DSLVEVKPGEIG+VSGIPEEHLTRRVVIYSPA
Sbjct: 1   MANSLQRIWNRGCALRGTMAPLCRSFSADSLVEVKPGEIGMVSGIPEEHLTRRVVIYSPA 60

Query: 61  RTASQQGSGKVGKWKINFVSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120
           RTASQQGSGKVGKWKINF+STQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK
Sbjct: 61  RTASQQGSGKVGKWKINFMSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120

Query: 121 HGWEYVVKKRHTPLLKAKAYADNFKWKGPP 151
           HGWEYVVKKRHTPLLKAKAYADNFKWKG P
Sbjct: 121 HGWEYVVKKRHTPLLKAKAYADNFKWKGHP 150

[Close]

BLAST of Chy2G031000 vs. ExPASy TrEMBL
Match: A0A6J1KR46 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111496890 PE=3 SV=1)

HSP 1 Score: 293.9 bits (751), Expect = 4.0e-76
Identity = 141/150 (94.00%), Postives = 145/150 (96.67%), Query Frame = 0


Query: 1   MANSLQRIWSHGRALRGTVAPLSRSFSTDSLVEVKPGEIGIVSGIPEEHLTRRVVIYSPA 60
           MANSLQRIW+ G ALRGT+APL RSFS DSLVEVKPGEIG+VSGIPEEHLTRRVVIYSPA
Sbjct: 1   MANSLQRIWNRGCALRGTMAPLCRSFSADSLVEVKPGEIGMVSGIPEEHLTRRVVIYSPA 60

Query: 61  RTASQQGSGKVGKWKINFVSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120
           RTASQQGSGKVGKWKINF+STQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK
Sbjct: 61  RTASQQGSGKVGKWKINFMSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120

Query: 121 HGWEYVVKKRHTPLLKAKAYADNFKWKGPP 151
           HGWEYVVKKRHTPLLKAKAYADNFKWKG P
Sbjct: 121 HGWEYVVKKRHTPLLKAKAYADNFKWKGHP 150

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BLAST of Chy2G031000 vs. NCBI nr
Match: XP_004141860.1 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial [Cucumis sativus] >KGN48618.1 hypothetical protein Csa_004287 [Cucumis sativus])

HSP 1 Score: 319 bits (818), Expect = 5.00e-110
Identity = 153/156 (98.08%), Postives = 156/156 (100.00%), Query Frame = 0


Query: 1   MANSLQRIWSHGRALRGTVAPLSRSFSTDSLVEVKPGEIGIVSGIPEEHLTRRVVIYSPA 60
           MANSLQRIWSHGRALRGT+APLSRSFSTDSLVE+KPGEIG+VSGIPEEHLTRRVVIYSPA
Sbjct: 1   MANSLQRIWSHGRALRGTLAPLSRSFSTDSLVEMKPGEIGVVSGIPEEHLTRRVVIYSPA 60

Query: 61  RTASQQGSGKVGKWKINFVSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120
           RTASQQGSGKVGKWKINFVSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK
Sbjct: 61  RTASQQGSGKVGKWKINFVSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120

Query: 121 HGWEYVVKKRHTPLLKAKAYADNFKWKGPPAAAKEA 156
           HGWEYVVKKRHTPLLKAKAYADNFKWKGPPAAAKEA
Sbjct: 121 HGWEYVVKKRHTPLLKAKAYADNFKWKGPPAAAKEA 156

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BLAST of Chy2G031000 vs. NCBI nr
Match: XP_008440406.1 (PREDICTED: NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial [Cucumis melo] >KAA0036408.1 NADH dehydrogenase (ubiquinone) iron-sulfur protein 4 [Cucumis melo var. makuwa] >TYK12804.1 NADH dehydrogenase (ubiquinone) iron-sulfur protein 4 [Cucumis melo var. makuwa])

HSP 1 Score: 315 bits (808), Expect = 1.68e-108
Identity = 152/156 (97.44%), Postives = 154/156 (98.72%), Query Frame = 0


Query: 1   MANSLQRIWSHGRALRGTVAPLSRSFSTDSLVEVKPGEIGIVSGIPEEHLTRRVVIYSPA 60
           MANSLQRIWSHGRALR TVAPLSRSFSTDSLV+VKPGEIG+VSGIPEEHLTRRVVIYSPA
Sbjct: 1   MANSLQRIWSHGRALRSTVAPLSRSFSTDSLVQVKPGEIGMVSGIPEEHLTRRVVIYSPA 60

Query: 61  RTASQQGSGKVGKWKINFVSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120
           RTASQQGSGKVGKWKINFVSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK
Sbjct: 61  RTASQQGSGKVGKWKINFVSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120

Query: 121 HGWEYVVKKRHTPLLKAKAYADNFKWKGPPAAAKEA 156
           HGWEYVVKKRHTPLLKAKAYADNFKWKGPPA AKEA
Sbjct: 121 HGWEYVVKKRHTPLLKAKAYADNFKWKGPPAEAKEA 156

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BLAST of Chy2G031000 vs. NCBI nr
Match: XP_038881462.1 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial [Benincasa hispida])

HSP 1 Score: 302 bits (773), Expect = 3.53e-103
Identity = 146/156 (93.59%), Postives = 151/156 (96.79%), Query Frame = 0


Query: 1   MANSLQRIWSHGRALRGTVAPLSRSFSTDSLVEVKPGEIGIVSGIPEEHLTRRVVIYSPA 60
           MANSLQRIWSHG ALRGTVAPL RSFSTDSLV+VKPGEIG+VSGIPEEHLTRRV+IYSPA
Sbjct: 1   MANSLQRIWSHGCALRGTVAPLCRSFSTDSLVQVKPGEIGMVSGIPEEHLTRRVIIYSPA 60

Query: 61  RTASQQGSGKVGKWKINFVSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120
           RTASQQGSGKVGKWKINF+STQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK
Sbjct: 61  RTASQQGSGKVGKWKINFMSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120

Query: 121 HGWEYVVKKRHTPLLKAKAYADNFKWKGPPAAAKEA 156
           HGWEYVV+KRHTPLLK KAYADNFKWKGPP A KEA
Sbjct: 121 HGWEYVVQKRHTPLLKVKAYADNFKWKGPPEA-KEA 155

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BLAST of Chy2G031000 vs. NCBI nr
Match: XP_022962977.1 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial-like [Cucurbita moschata] >XP_022962978.1 NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial-like [Cucurbita moschata] >XP_023003205.1 NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial-like [Cucurbita maxima] >XP_023517797.1 NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial-like [Cucurbita pepo subsp. pepo] >KAG6594972.1 NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 294 bits (752), Expect = 5.62e-100
Identity = 141/150 (94.00%), Postives = 145/150 (96.67%), Query Frame = 0


Query: 1   MANSLQRIWSHGRALRGTVAPLSRSFSTDSLVEVKPGEIGIVSGIPEEHLTRRVVIYSPA 60
           MANSLQRIW+ G ALRGT+APL RSFS DSLVEVKPGEIG+VSGIPEEHLTRRVVIYSPA
Sbjct: 1   MANSLQRIWNRGCALRGTMAPLCRSFSADSLVEVKPGEIGMVSGIPEEHLTRRVVIYSPA 60

Query: 61  RTASQQGSGKVGKWKINFVSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120
           RTASQQGSGKVGKWKINF+STQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK
Sbjct: 61  RTASQQGSGKVGKWKINFMSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120

Query: 121 HGWEYVVKKRHTPLLKAKAYADNFKWKGPP 150
           HGWEYVVKKRHTPLLKAKAYADNFKWKG P
Sbjct: 121 HGWEYVVKKRHTPLLKAKAYADNFKWKGHP 150

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BLAST of Chy2G031000 vs. NCBI nr
Match: XP_022132610.1 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial [Momordica charantia])

HSP 1 Score: 294 bits (752), Expect = 5.62e-100
Identity = 142/156 (91.03%), Postives = 148/156 (94.87%), Query Frame = 0


Query: 1   MANSLQRIWSHGRALRGTVAPLSRSFSTDSLVEVKPGEIGIVSGIPEEHLTRRVVIYSPA 60
           MANSLQRIW+H   LRGT+APL RSFS DSLV+VKPGEIG+VSGIPEEHLTRRVVIYSPA
Sbjct: 1   MANSLQRIWNHRCGLRGTLAPLCRSFSADSLVQVKPGEIGMVSGIPEEHLTRRVVIYSPA 60

Query: 61  RTASQQGSGKVGKWKINFVSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120
           RTASQQGSGKVGKWKINF+STQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK
Sbjct: 61  RTASQQGSGKVGKWKINFMSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEK 120

Query: 121 HGWEYVVKKRHTPLLKAKAYADNFKWKGPPAAAKEA 156
           HGW+YVVKKRHTPLLK KAYADNFKWKGPP A KEA
Sbjct: 121 HGWDYVVKKRHTPLLKVKAYADNFKWKGPPEA-KEA 155

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BLAST of Chy2G031000 vs. TAIR 10
Match: AT5G67590.1 (NADH-ubiquinone oxidoreductase-related )

HSP 1 Score: 220.3 bits (560), Expect = 1.1e-57
Identity = 103/138 (74.64%), Postives = 122/138 (88.41%), Query Frame = 0


Query: 15  LRGTVAPLSRSFSTDSLVE--VKPGEIGIVSGIPEEHLTRRVVIYSPARTASQQGSGKVG 74
           +  T+  ++R F+TD++VE   K GEIG VSGIPEEHL+R+V+IYSPARTA+Q GSGK+G
Sbjct: 13  IAATLRRVARPFATDAVVESDYKRGEIGKVSGIPEEHLSRKVIIYSPARTATQSGSGKLG 72

Query: 75  KWKINFVSTQKWENPLMGWTSTGDPYANVGDSALSFDSEEAAKAFAEKHGWEYVVKKRHT 134
           KWKINFVST KWENPLMGWTSTGDPYANVGDSAL+FDSEEAAK+FAE+HGW+Y VKK +T
Sbjct: 73  KWKINFVSTLKWENPLMGWTSTGDPYANVGDSALAFDSEEAAKSFAERHGWDYKVKKPNT 132

Query: 135 PLLKAKAYADNFKWKGPP 151
           PLLK K+Y+DNFKWKG P
Sbjct: 133 PLLKVKSYSDNFKWKGNP 150

The following BLAST results are available for this feature:

BLAST of Chy2G031000 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FJW4	1.5e-56	74.64	NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Arabidop...	[more]
Q5XIF3	9.0e-25	46.28	NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Rattus n...	[more]
Q02375	3.4e-24	47.27	NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Bos taur...	[more]
Q0MQH0	3.4e-24	42.34	NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Gorilla ...	[more]
O43181	3.4e-24	42.34	NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Homo sap...	[more]

BLAST of Chy2G031000 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KGI3	1.2e-83	98.08	NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Cucumis ...	[more]
A0A5D3CP24	1.7e-82	97.44	NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Cucumis ...	[more]
A0A1S3B1N9	1.7e-82	97.44	NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Cucumis ...	[more]
A0A6J1HE01	4.0e-76	94.00	NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Cucurbit...	[more]
A0A6J1KR46	4.0e-76	94.00	NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial OS=Cucurbit...	[more]

BLAST of Chy2G031000 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004141860.1	5.00e-110	98.08	NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial [Cucumis sa...	[more]
XP_008440406.1	1.68e-108	97.44	PREDICTED: NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial ...	[more]
XP_038881462.1	3.53e-103	93.59	NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial [Benincasa ...	[more]
XP_022962977.1	5.62e-100	94.00	NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial-like [Cucur...	[more]
XP_022132610.1	5.62e-100	91.03	NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial [Momordica ...	[more]

BLAST of Chy2G031000 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G67590.1	1.1e-57	74.64	NADH-ubiquinone oxidoreductase-related	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006885	NADH dehydrogenase ubiquinone Fe-S protein 4, mitochondrial	PFAM	PF04800	ETC_C1_NDUFA4	coord: 53..147 e-value: 5.9E-33 score: 113.0
IPR006885	NADH dehydrogenase ubiquinone Fe-S protein 4, mitochondrial	PANTHER	PTHR12219	NADH-UBIQUINONE OXIDOREDUCTASE	coord: 14..152
IPR038532	NADH dehydrogenase ubiquinone Fe-S protein 4-like superfamily	GENE3D	3.30.160.190	atu1810 like domain	coord: 52..127 e-value: 6.5E-34 score: 117.7
None	No IPR available	PANTHER	PTHR12219:SF23	NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 4, MITOCHONDRIAL-LIKE	coord: 14..152

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy2G031000.1	Chy2G031000.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0022900	electron transport chain
cellular_component	GO:0005743	mitochondrial inner membrane
cellular_component	GO:0070469	respirasome
molecular_function	GO:0016651	oxidoreductase activity, acting on NAD(P)H