Chy2G030270 (gene) Cucumber (hystrix) v1

Overview


Name	Chy2G030270
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	nicotinamidase 1
Location	chrH02: 4712302 .. 4714619 (-)
RNA-Seq Expression	Chy2G030270
Synteny	Chy2G030270

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTCTTAGAGGTCATGGAGCTGTTGAAGGAGCACCTTCCTCTGACTCAAGAGCCTTTCGTTTTGTCAGGTGACCTCAACATCGGCCTCGTCCTCGTCGATGTCGTTAATGGCTTCTGCACCGTCGGCGCTGGAAATTTGGTAATTCCATCTCCTCCTTACTTTTCTTCTTCTCGTTCACCAATTTTAAATTTCGATTGATCACGGAGATTCCTATGAGTTTTGGTGATTCCGTCGATTTCATTAGATTGTGACGGCTTGTTTTACAGGCTCCGAAACAGCACAACGAGCAAATTTCTCAAATGGTGGAGGAATCGGCAAGACTCGCCAGAGTTTTCTGCGAGAAAAAATGGCCTATATTCGCGTTTTGTGATTCTCATCATCCGGACATTCCGGAGCCTCCCTATCCTCCTCATTGCATCGCTGGAACAGACGAATCCAAATTAGTTCCAGGTACGTACTGCTAATTTCGTGGTTGAATCTAACTTTAGCAATTTGCAATGAAAATACAAAGCCAATTTTTGAAAACCTACCGTACTTGTTTGCAGCCCTGCAATGGCTGGAAAACGAGGCGAATGTGACGCTGAGATGCAAAGATTGCATCGATGGATTCTTAGGTTGTTTGGAAAAGGATGGCTCTAACATTTTCATCGATTGGGTGAAGAAAAATCAGATCAAAGGCGTGAGTCTCTCTAGTATTCAATCGTAGATCCAAATTGGTTTAGTTACGTATTCTTTTTTTGTTTATTCAATCAGAGAGAAGTAATTTTGGATACGCTTTCTTCTTTTGAACATGAATAGATCCTAGTGCTGGGAATTTGCACGGACATATGCGTGCTGGATTTTGTATGTTCAACATTATCAGCAAGAAATCGTGGTTTCCTTTCACCATTGGAGGATGTGATAGTATATTCCGGTGGCTGCGCCACTTATGATCTTCCCGTAGCTGTGGCCAAGACTTTAGGAGATGCCATTGCTCACCCACAGGTACCCAAATTCTTAATTCATTGTTGACTTTCATACAAATTAAGCCTCTCTTATATTTAATATTTAATATCATGAAAAAGAACTGCTTTGGTTTTGTTTTTATCTTGTTCTTTATTTCTCGCTTTTTTTGTCTCTTCCTTAGTTCAAACCTTATTTTTATTGGAGTCAATAGGACCTGCTGAAATCACACATCACTTGCTTTACTTTATTGTTTTTCTAATTGTTGTAGTGAATTAGAGAGATTCTAATCAGTGATACAGTACATTTTTTCTGGTTTAAGCAATGTTAGTAATATTGGTTCAGGAATTGATGCACCACATAGGCCTGTACATAGCAAGGGGGAGAGGAGCCAAGGTGGTATCGAAGGTTTCAATTAAATCACTTTGAGGAAGCCACTTAGTTTAATTAATTAAGCTAGCTCTGCTCTAGAGAGGAACATAAAGACATCTTGTGTATGGAAGCCCATTTAGTTCACTCAGTACATGTAACCATGAACCTTCTTGGGCAAATGAAGATTGTACAAGTTTAGTTCTTATTTTGAGTGTATAGTCTCTTCAACTTCGACTTTAAAAAATATCTACTAAATTAATTTAACCTATGTTCAGCAGATCTTTAAAACTAATTCTAACATTAATTCTTAAAGACCCCATCTGAAATTCATTTGATTTTTTAAAAAAAATTAAACCAAACACTACTTCCAACTCCAACTCTCTTCTTTTGTTTTTCATATTCTACCAATGGTTATAAAATGTCAAGCAAAAATTTAAATAATAAAAAAAAAAAGTAGCTTTCAAAAAGTTTCTTGGTTTTGAAAATAGGATAAGAATTCAATCATTATATTTAAAAAAATGCAAATTGTTGTAAGACAGGAGAGAAAATATGTTTAATTTAAAAAAATGGAAGTGGCTAAAAAACGGAACCTTATGTTTAATAGATATTTGAATTAAGGAAAAGAGGGGAGGATGAACAAGTCCATAAGAGATAATTATAGGAAATAGGATAAAAGAAACGGTAGTAGTTTGTATATGGACTGTATACCATATATAGTGTATACAATAAGGGGCGAAAGAAAACAAAAATAAAAAGAAAGAACATCCCCTTTCCAATGGTTTCCAGATTGTCAATCAAATTTATGTATGCAAAAAACAGGAGCATGGAAAGAAAGATCTTGATATGCACAAAACAAAACTTAGGTCTGTTATTCTATTTTATAAACTGCTACTCAATAGGATACTAAACTTTGGGGTAATGAGCATATGTGCTTCAATGTGGTCGCCGTTGTCACCGAGAGTACTTCAGGCTACCGAGTTTTGCAGAGAAGAAAGCCATAG

mRNA sequence

ATGGTCTTAGAGGTCATGGAGCTGTTGAAGGAGCACCTTCCTCTGACTCAAGAGCCTTTCGTTTTGTCAGGTGACCTCAACATCGGCCTCGTCCTCGTCGATGTCGTTAATGGCTTCTGCACCGTCGGCGCTGGAAATTTGGCTCCGAAACAGCACAACGAGCAAATTTCTCAAATGGTGGAGGAATCGGCAAGACTCGCCAGAGTTTTCTGCGAGAAAAAATGGCCTATATTCGCGTTTTGTGATTCTCATCATCCGGACATTCCGGAGCCTCCCTATCCTCCTCATTGCATCGCTGGAACAGACGAATCCAAATTAGTTCCAGCCCTGCAATGGCTGGAAAACGAGGCGAATGTGACGCTGAGATGCAAAGATTGCATCGATGGATTCTTAGGTTGTTTGGAAAAGGATGGCTCTAACATTTTCATCGATTGGGTGAAGAAAAATCAGATCAAAGGCATCCTAGTGCTGGGAATTTGCACGGACATATGCGTGCTGGATTTTGTATGTTCAACATTATCAGCAAGAAATCGTGGTTTCCTTTCACCATTGGAGGATGTGATAGTATATTCCGGTGGCTGCGCCACTTATGATCTTCCCGTAGCTGTGGCCAAGACTTTAGGAGATGCCATTGCTCACCCACAGGAATTGATGCACCACATAGGCCTGTACATAGCAAGGGGGAGAGGAGCCAAGGTGGTATCGAAGGATACTAAACTTTGGGGTAATGAGCATATGTGCTTCAATGTGGTCGCCGTTGTCACCGAGAGTACTTCAGGCTACCGAGTTTTGCAGAGAAGAAAGCCATAG

Coding sequence (CDS)

ATGGTCTTAGAGGTCATGGAGCTGTTGAAGGAGCACCTTCCTCTGACTCAAGAGCCTTTCGTTTTGTCAGGTGACCTCAACATCGGCCTCGTCCTCGTCGATGTCGTTAATGGCTTCTGCACCGTCGGCGCTGGAAATTTGGCTCCGAAACAGCACAACGAGCAAATTTCTCAAATGGTGGAGGAATCGGCAAGACTCGCCAGAGTTTTCTGCGAGAAAAAATGGCCTATATTCGCGTTTTGTGATTCTCATCATCCGGACATTCCGGAGCCTCCCTATCCTCCTCATTGCATCGCTGGAACAGACGAATCCAAATTAGTTCCAGCCCTGCAATGGCTGGAAAACGAGGCGAATGTGACGCTGAGATGCAAAGATTGCATCGATGGATTCTTAGGTTGTTTGGAAAAGGATGGCTCTAACATTTTCATCGATTGGGTGAAGAAAAATCAGATCAAAGGCATCCTAGTGCTGGGAATTTGCACGGACATATGCGTGCTGGATTTTGTATGTTCAACATTATCAGCAAGAAATCGTGGTTTCCTTTCACCATTGGAGGATGTGATAGTATATTCCGGTGGCTGCGCCACTTATGATCTTCCCGTAGCTGTGGCCAAGACTTTAGGAGATGCCATTGCTCACCCACAGGAATTGATGCACCACATAGGCCTGTACATAGCAAGGGGGAGAGGAGCCAAGGTGGTATCGAAGGATACTAAACTTTGGGGTAATGAGCATATGTGCTTCAATGTGGTCGCCGTTGTCACCGAGAGTACTTCAGGCTACCGAGTTTTGCAGAGAAGAAAGCCATAG

Protein sequence

MVLEVMELLKEHLPLTQEPFVLSGDLNIGLVLVDVVNGFCTVGAGNLAPKQHNEQISQMVEESARLARVFCEKKWPIFAFCDSHHPDIPEPPYPPHCIAGTDESKLVPALQWLENEANVTLRCKDCIDGFLGCLEKDGSNIFIDWVKKNQIKGILVLGICTDICVLDFVCSTLSARNRGFLSPLEDVIVYSGGCATYDLPVAVAKTLGDAIAHPQELMHHIGLYIARGRGAKVVSKDTKLWGNEHMCFNVVAVVTESTSGYRVLQRRKP*

Homology

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BLAST of Chy2G030270 vs. ExPASy Swiss-Prot
Match: Q8S8F9 (Nicotinamidase 1 OS=Arabidopsis thaliana OX=3702 GN=NIC1 PE=1 SV=1)

HSP 1 Score: 352.8 bits (904), Expect = 3.4e-96
Identity = 158/233 (67.81%), Postives = 200/233 (85.84%), Query Frame = 0


Query: 5   VMELLKEHLPL-TQEPFVLSGDLNIGLVLVDVVNGFCTVGAGNLAPKQHNEQISQMVEES 64
           + + LK+ +P+  +EP +L+ D ++GLV+VDVVNGFCT+G+GN+AP +HNEQIS+MVEES
Sbjct: 7   IFDQLKKQIPVDEEEPLILNRDSSVGLVIVDVVNGFCTIGSGNMAPTKHNEQISKMVEES 66

Query: 65  ARLARVFCEKKWPIFAFCDSHHPDIPEPPYPPHCIAGTDESKLVPALQWLENEANVTLRC 124
           A+LAR FC++KWP+ AF DSHHPDIPE PYPPHCI GT+ES+LVPAL+WLE+E   TLR 
Sbjct: 67  AKLAREFCDRKWPVLAFIDSHHPDIPERPYPPHCIIGTEESELVPALKWLESEDCATLRR 126

Query: 125 KDCIDGFLGCLEKDGSNIFIDWVKKNQIKGILVLGICTDICVLDFVCSTLSARNRGFLSP 184
           KDCI+GF+G +E DGSN+F+DWVK+ QIK I+V+GICTDICV DFV + LSARN G LSP
Sbjct: 127 KDCINGFVGSMESDGSNVFVDWVKEKQIKVIVVVGICTDICVFDFVATALSARNHGVLSP 186

Query: 185 LEDVIVYSGGCATYDLPVAVAKTLGDAIAHPQELMHHIGLYIARGRGAKVVSK 237
           +EDV+VYS GCAT+DLP+ VAK +  A AHPQELMHH+GLY+A+GRGA+VVSK
Sbjct: 187 VEDVVVYSRGCATFDLPLHVAKDIKGAQAHPQELMHHVGLYMAKGRGAQVVSK 239

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BLAST of Chy2G030270 vs. ExPASy Swiss-Prot
Match: O32091 (Uncharacterized isochorismatase family protein PncA OS=Bacillus subtilis (strain 168) OX=224308 GN=pncA PE=3 SV=1)

HSP 1 Score: 47.8 bits (112), Expect = 2.3e-04
Identity = 36/104 (34.62%), Postives = 51/104 (49.04%), Query Frame = 0


Query: 82  DSHHPDIPEPPYPPHCIAGTD----ESKLVPALQWLENEANVTLRCKDCIDGFLGC-LEK 141
           D +HP+     +PPH I GT+      KL+P  Q  E+E NV    K     F G  LE 
Sbjct: 59  DQYHPE--TRLFPPHNIKGTEGKDLYGKLLPLYQKHEHEPNVYYMEKTRYSAFAGTDLEL 118

Query: 142 DGSNIFIDWVKKNQIKGILVLGICTDICVLDFVCSTLSARNRGF 181
                    +++ QI  + + G+CTDICVL    + + A N+GF
Sbjct: 119 K--------LRERQIGELHLAGVCTDICVLH---TAVDAYNKGF 149

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BLAST of Chy2G030270 vs. ExPASy TrEMBL
Match: A0A0A0KG59 (Catalytic OS=Cucumis sativus OX=3659 GN=Csa_6G498960 PE=3 SV=1)

HSP 1 Score: 486.9 bits (1252), Expect = 5.5e-134
Identity = 233/236 (98.73%), Postives = 235/236 (99.58%), Query Frame = 0


Query: 1   MVLEVMELLKEHLPLTQEPFVLSGDLNIGLVLVDVVNGFCTVGAGNLAPKQHNEQISQMV 60
           MVLEVMELLKEHLPLTQEPFVLSGDLNIGLVLVDVVNGFCTVGAGNLAPKQHNEQISQMV
Sbjct: 1   MVLEVMELLKEHLPLTQEPFVLSGDLNIGLVLVDVVNGFCTVGAGNLAPKQHNEQISQMV 60

Query: 61  EESARLARVFCEKKWPIFAFCDSHHPDIPEPPYPPHCIAGTDESKLVPALQWLENEANVT 120
           EESARLARVFCEKKWPIFAF DSHHPDIPEPPYPPHCIAGTDESKLVPALQWLENEANVT
Sbjct: 61  EESARLARVFCEKKWPIFAFRDSHHPDIPEPPYPPHCIAGTDESKLVPALQWLENEANVT 120

Query: 121 LRCKDCIDGFLGCLEKDGSNIFIDWVKKNQIKGILVLGICTDICVLDFVCSTLSARNRGF 180
           LRCKDCIDGFLGCLEKDGSNIFIDWVKKNQIKGILVLGICTDICVLDFVCSTLSARNRGF
Sbjct: 121 LRCKDCIDGFLGCLEKDGSNIFIDWVKKNQIKGILVLGICTDICVLDFVCSTLSARNRGF 180

Query: 181 LSPLEDVIVYSGGCATYDLPVAVAKTLGDAIAHPQELMHHIGLYIARGRGAKVVSK 237
           LSPLEDVIVYSGGCATYDLPVAVAKTLGDAIAHPQELMHH+GLYIARGRGAKVVS+
Sbjct: 181 LSPLEDVIVYSGGCATYDLPVAVAKTLGDAIAHPQELMHHVGLYIARGRGAKVVSE 236

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BLAST of Chy2G030270 vs. ExPASy TrEMBL
Match: A0A5D3CLP4 (Nicotinamidase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G003290 PE=3 SV=1)

HSP 1 Score: 474.2 bits (1219), Expect = 3.7e-130
Identity = 226/236 (95.76%), Postives = 232/236 (98.31%), Query Frame = 0


Query: 1   MVLEVMELLKEHLPLTQEPFVLSGDLNIGLVLVDVVNGFCTVGAGNLAPKQHNEQISQMV 60
           MVLEVMELLKEHLP+TQEPFVLSGD+N GLVLVDVVNGFCTVGAGNLAPKQHN+QISQMV
Sbjct: 1   MVLEVMELLKEHLPVTQEPFVLSGDVNTGLVLVDVVNGFCTVGAGNLAPKQHNKQISQMV 60

Query: 61  EESARLARVFCEKKWPIFAFCDSHHPDIPEPPYPPHCIAGTDESKLVPALQWLENEANVT 120
           EESARLARVFCEKKWPIFAF DSHHPDIPE PYPPHCIAGTDESKLVPALQWLENEANVT
Sbjct: 61  EESARLARVFCEKKWPIFAFLDSHHPDIPEHPYPPHCIAGTDESKLVPALQWLENEANVT 120

Query: 121 LRCKDCIDGFLGCLEKDGSNIFIDWVKKNQIKGILVLGICTDICVLDFVCSTLSARNRGF 180
           LRCKDCIDGFLGCLEKDGSNIFIDWVKKNQIK ILVLGICTDICVLDFVCSTLSARNRGF
Sbjct: 121 LRCKDCIDGFLGCLEKDGSNIFIDWVKKNQIKNILVLGICTDICVLDFVCSTLSARNRGF 180

Query: 181 LSPLEDVIVYSGGCATYDLPVAVAKTLGDAIAHPQELMHHIGLYIARGRGAKVVSK 237
           LSPLEDVIVYSGGCATYDLPV+VAKTLGDAIAHPQELMHHIGLY+ARGRGAKVVS+
Sbjct: 181 LSPLEDVIVYSGGCATYDLPVSVAKTLGDAIAHPQELMHHIGLYMARGRGAKVVSE 236

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BLAST of Chy2G030270 vs. ExPASy TrEMBL
Match: A0A1S3B1Y8 (nicotinamidase 1 OS=Cucumis melo OX=3656 GN=LOC103484929 PE=3 SV=1)

HSP 1 Score: 474.2 bits (1219), Expect = 3.7e-130
Identity = 226/236 (95.76%), Postives = 232/236 (98.31%), Query Frame = 0


Query: 1   MVLEVMELLKEHLPLTQEPFVLSGDLNIGLVLVDVVNGFCTVGAGNLAPKQHNEQISQMV 60
           MVLEVMELLKEHLP+TQEPFVLSGD+N GLVLVDVVNGFCTVGAGNLAPKQHN+QISQMV
Sbjct: 1   MVLEVMELLKEHLPVTQEPFVLSGDVNTGLVLVDVVNGFCTVGAGNLAPKQHNKQISQMV 60

Query: 61  EESARLARVFCEKKWPIFAFCDSHHPDIPEPPYPPHCIAGTDESKLVPALQWLENEANVT 120
           EESARLARVFCEKKWPIFAF DSHHPDIPE PYPPHCIAGTDESKLVPALQWLENEANVT
Sbjct: 61  EESARLARVFCEKKWPIFAFLDSHHPDIPEHPYPPHCIAGTDESKLVPALQWLENEANVT 120

Query: 121 LRCKDCIDGFLGCLEKDGSNIFIDWVKKNQIKGILVLGICTDICVLDFVCSTLSARNRGF 180
           LRCKDCIDGFLGCLEKDGSNIFIDWVKKNQIK ILVLGICTDICVLDFVCSTLSARNRGF
Sbjct: 121 LRCKDCIDGFLGCLEKDGSNIFIDWVKKNQIKNILVLGICTDICVLDFVCSTLSARNRGF 180

Query: 181 LSPLEDVIVYSGGCATYDLPVAVAKTLGDAIAHPQELMHHIGLYIARGRGAKVVSK 237
           LSPLEDVIVYSGGCATYDLPV+VAKTLGDAIAHPQELMHHIGLY+ARGRGAKVVS+
Sbjct: 181 LSPLEDVIVYSGGCATYDLPVSVAKTLGDAIAHPQELMHHIGLYMARGRGAKVVSE 236

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BLAST of Chy2G030270 vs. ExPASy TrEMBL
Match: A0A6J1KQH8 (nicotinamidase 1 OS=Cucurbita maxima OX=3661 GN=LOC111496727 PE=3 SV=1)

HSP 1 Score: 451.4 bits (1160), Expect = 2.6e-123
Identity = 211/236 (89.41%), Postives = 227/236 (96.19%), Query Frame = 0


Query: 1   MVLEVMELLKEHLPLTQEPFVLSGDLNIGLVLVDVVNGFCTVGAGNLAPKQHNEQISQMV 60
           MV EV+ELLKE+LP+TQEPFVLSGD+N GLVLVDVVNGFCTVGAGNLAPKQH++QISQMV
Sbjct: 3   MVSEVVELLKENLPVTQEPFVLSGDVNTGLVLVDVVNGFCTVGAGNLAPKQHDKQISQMV 62

Query: 61  EESARLARVFCEKKWPIFAFCDSHHPDIPEPPYPPHCIAGTDESKLVPALQWLENEANVT 120
           EESARLARVFCEKKWPIFAF DSHHPDIPEPPYPPHCIAGTDE+KLVPALQWLENE NVT
Sbjct: 63  EESARLARVFCEKKWPIFAFLDSHHPDIPEPPYPPHCIAGTDEAKLVPALQWLENETNVT 122

Query: 121 LRCKDCIDGFLGCLEKDGSNIFIDWVKKNQIKGILVLGICTDICVLDFVCSTLSARNRGF 180
           L+CKDCIDGF+GCLEKDGSN+FIDWVK NQIK ILVLGICTDICVLDFVCSTLSARNRGF
Sbjct: 123 LKCKDCIDGFVGCLEKDGSNVFIDWVKNNQIKVILVLGICTDICVLDFVCSTLSARNRGF 182

Query: 181 LSPLEDVIVYSGGCATYDLPVAVAKTLGDAIAHPQELMHHIGLYIARGRGAKVVSK 237
           L PL+DVI+YSGGCAT+DLPV+VAK LGDAIAHPQ LMHHIGLY+ARGRGAKVVS+
Sbjct: 183 LPPLKDVIIYSGGCATFDLPVSVAKNLGDAIAHPQALMHHIGLYLARGRGAKVVSE 238

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BLAST of Chy2G030270 vs. ExPASy TrEMBL
Match: A0A6J1HG67 (nicotinamidase 1 OS=Cucurbita moschata OX=3662 GN=LOC111463275 PE=3 SV=1)

HSP 1 Score: 447.6 bits (1150), Expect = 3.7e-122
Identity = 209/236 (88.56%), Postives = 226/236 (95.76%), Query Frame = 0


Query: 1   MVLEVMELLKEHLPLTQEPFVLSGDLNIGLVLVDVVNGFCTVGAGNLAPKQHNEQISQMV 60
           MV EV+ELLKE+LP+TQEPFVLSGD+N GLVLVDVVNGFCTVGAGNLAPKQH+EQISQMV
Sbjct: 1   MVSEVVELLKENLPVTQEPFVLSGDVNTGLVLVDVVNGFCTVGAGNLAPKQHDEQISQMV 60

Query: 61  EESARLARVFCEKKWPIFAFCDSHHPDIPEPPYPPHCIAGTDESKLVPALQWLENEANVT 120
           EESARLARVFCEKKWPIFAF DSH PDIPEPPYPPHCIAGTDE+KLVPALQWLENE NVT
Sbjct: 61  EESARLARVFCEKKWPIFAFLDSHRPDIPEPPYPPHCIAGTDEAKLVPALQWLENETNVT 120

Query: 121 LRCKDCIDGFLGCLEKDGSNIFIDWVKKNQIKGILVLGICTDICVLDFVCSTLSARNRGF 180
           L+CKDCIDGF+GCLEKDGSN+F+DWVK NQIK ILVLGICTDICVLDFVCSTLSARNRGF
Sbjct: 121 LKCKDCIDGFVGCLEKDGSNVFVDWVKNNQIKVILVLGICTDICVLDFVCSTLSARNRGF 180

Query: 181 LSPLEDVIVYSGGCATYDLPVAVAKTLGDAIAHPQELMHHIGLYIARGRGAKVVSK 237
           L PL+DVI+YSGGCAT+DLPV+VAK LG+AIAHPQ LMHHIGLY+ARGRGAKVVS+
Sbjct: 181 LPPLKDVIIYSGGCATFDLPVSVAKNLGNAIAHPQALMHHIGLYLARGRGAKVVSE 236

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BLAST of Chy2G030270 vs. NCBI nr
Match: XP_004143443.1 (nicotinamidase 1 [Cucumis sativus] >KGN48700.1 hypothetical protein Csa_003282 [Cucumis sativus])

HSP 1 Score: 481 bits (1237), Expect = 1.37e-170
Identity = 233/236 (98.73%), Postives = 235/236 (99.58%), Query Frame = 0


Query: 1   MVLEVMELLKEHLPLTQEPFVLSGDLNIGLVLVDVVNGFCTVGAGNLAPKQHNEQISQMV 60
           MVLEVMELLKEHLPLTQEPFVLSGDLNIGLVLVDVVNGFCTVGAGNLAPKQHNEQISQMV
Sbjct: 1   MVLEVMELLKEHLPLTQEPFVLSGDLNIGLVLVDVVNGFCTVGAGNLAPKQHNEQISQMV 60

Query: 61  EESARLARVFCEKKWPIFAFCDSHHPDIPEPPYPPHCIAGTDESKLVPALQWLENEANVT 120
           EESARLARVFCEKKWPIFAF DSHHPDIPEPPYPPHCIAGTDESKLVPALQWLENEANVT
Sbjct: 61  EESARLARVFCEKKWPIFAFRDSHHPDIPEPPYPPHCIAGTDESKLVPALQWLENEANVT 120

Query: 121 LRCKDCIDGFLGCLEKDGSNIFIDWVKKNQIKGILVLGICTDICVLDFVCSTLSARNRGF 180
           LRCKDCIDGFLGCLEKDGSNIFIDWVKKNQIKGILVLGICTDICVLDFVCSTLSARNRGF
Sbjct: 121 LRCKDCIDGFLGCLEKDGSNIFIDWVKKNQIKGILVLGICTDICVLDFVCSTLSARNRGF 180

Query: 181 LSPLEDVIVYSGGCATYDLPVAVAKTLGDAIAHPQELMHHIGLYIARGRGAKVVSK 236
           LSPLEDVIVYSGGCATYDLPVAVAKTLGDAIAHPQELMHH+GLYIARGRGAKVVS+
Sbjct: 181 LSPLEDVIVYSGGCATYDLPVAVAKTLGDAIAHPQELMHHVGLYIARGRGAKVVSE 236

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BLAST of Chy2G030270 vs. NCBI nr
Match: XP_008440534.1 (PREDICTED: nicotinamidase 1 [Cucumis melo] >KAA0036347.1 nicotinamidase 1 [Cucumis melo var. makuwa] >TYK12741.1 nicotinamidase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 468 bits (1204), Expect = 1.47e-165
Identity = 226/236 (95.76%), Postives = 232/236 (98.31%), Query Frame = 0


Query: 1   MVLEVMELLKEHLPLTQEPFVLSGDLNIGLVLVDVVNGFCTVGAGNLAPKQHNEQISQMV 60
           MVLEVMELLKEHLP+TQEPFVLSGD+N GLVLVDVVNGFCTVGAGNLAPKQHN+QISQMV
Sbjct: 1   MVLEVMELLKEHLPVTQEPFVLSGDVNTGLVLVDVVNGFCTVGAGNLAPKQHNKQISQMV 60

Query: 61  EESARLARVFCEKKWPIFAFCDSHHPDIPEPPYPPHCIAGTDESKLVPALQWLENEANVT 120
           EESARLARVFCEKKWPIFAF DSHHPDIPE PYPPHCIAGTDESKLVPALQWLENEANVT
Sbjct: 61  EESARLARVFCEKKWPIFAFLDSHHPDIPEHPYPPHCIAGTDESKLVPALQWLENEANVT 120

Query: 121 LRCKDCIDGFLGCLEKDGSNIFIDWVKKNQIKGILVLGICTDICVLDFVCSTLSARNRGF 180
           LRCKDCIDGFLGCLEKDGSNIFIDWVKKNQIK ILVLGICTDICVLDFVCSTLSARNRGF
Sbjct: 121 LRCKDCIDGFLGCLEKDGSNIFIDWVKKNQIKNILVLGICTDICVLDFVCSTLSARNRGF 180

Query: 181 LSPLEDVIVYSGGCATYDLPVAVAKTLGDAIAHPQELMHHIGLYIARGRGAKVVSK 236
           LSPLEDVIVYSGGCATYDLPV+VAKTLGDAIAHPQELMHHIGLY+ARGRGAKVVS+
Sbjct: 181 LSPLEDVIVYSGGCATYDLPVSVAKTLGDAIAHPQELMHHIGLYMARGRGAKVVSE 236

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BLAST of Chy2G030270 vs. NCBI nr
Match: XP_038881297.1 (nicotinamidase 1 [Benincasa hispida])

HSP 1 Score: 450 bits (1158), Expect = 1.51e-158
Identity = 216/236 (91.53%), Postives = 227/236 (96.19%), Query Frame = 0


Query: 1   MVLEVMELLKEHLPLTQEPFVLSGDLNIGLVLVDVVNGFCTVGAGNLAPKQHNEQISQMV 60
           MV EVMELLKEHLP+TQEPFVLSGD+N GLVLVDVVNGFCTVGAGNLAPKQH++QISQMV
Sbjct: 1   MVSEVMELLKEHLPVTQEPFVLSGDVNTGLVLVDVVNGFCTVGAGNLAPKQHDKQISQMV 60

Query: 61  EESARLARVFCEKKWPIFAFCDSHHPDIPEPPYPPHCIAGTDESKLVPALQWLENEANVT 120
           EES RLARVFCEKKWPIFAF DSHHPD+PE PYPPHCIAGTDESKLVPALQWLENEANVT
Sbjct: 61  EESTRLARVFCEKKWPIFAFLDSHHPDVPEDPYPPHCIAGTDESKLVPALQWLENEANVT 120

Query: 121 LRCKDCIDGFLGCLEKDGSNIFIDWVKKNQIKGILVLGICTDICVLDFVCSTLSARNRGF 180
           LRCKDCIDG LG LEKDGSN+FIDWVKKNQIK ILVLGICTDICVLDFVCSTLS RNRGF
Sbjct: 121 LRCKDCIDGLLGSLEKDGSNVFIDWVKKNQIKVILVLGICTDICVLDFVCSTLSTRNRGF 180

Query: 181 LSPLEDVIVYSGGCATYDLPVAVAKTLGDAIAHPQELMHHIGLYIARGRGAKVVSK 236
           LSPLEDVIVYSGGCAT+DLPV++AKTLGDAIAHPQELMHHIGLY+ARGRGAKVVS+
Sbjct: 181 LSPLEDVIVYSGGCATFDLPVSLAKTLGDAIAHPQELMHHIGLYMARGRGAKVVSE 236

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BLAST of Chy2G030270 vs. NCBI nr
Match: XP_023002980.1 (nicotinamidase 1 [Cucurbita maxima])

HSP 1 Score: 445 bits (1145), Expect = 1.55e-156
Identity = 211/236 (89.41%), Postives = 227/236 (96.19%), Query Frame = 0


Query: 1   MVLEVMELLKEHLPLTQEPFVLSGDLNIGLVLVDVVNGFCTVGAGNLAPKQHNEQISQMV 60
           MV EV+ELLKE+LP+TQEPFVLSGD+N GLVLVDVVNGFCTVGAGNLAPKQH++QISQMV
Sbjct: 3   MVSEVVELLKENLPVTQEPFVLSGDVNTGLVLVDVVNGFCTVGAGNLAPKQHDKQISQMV 62

Query: 61  EESARLARVFCEKKWPIFAFCDSHHPDIPEPPYPPHCIAGTDESKLVPALQWLENEANVT 120
           EESARLARVFCEKKWPIFAF DSHHPDIPEPPYPPHCIAGTDE+KLVPALQWLENE NVT
Sbjct: 63  EESARLARVFCEKKWPIFAFLDSHHPDIPEPPYPPHCIAGTDEAKLVPALQWLENETNVT 122

Query: 121 LRCKDCIDGFLGCLEKDGSNIFIDWVKKNQIKGILVLGICTDICVLDFVCSTLSARNRGF 180
           L+CKDCIDGF+GCLEKDGSN+FIDWVK NQIK ILVLGICTDICVLDFVCSTLSARNRGF
Sbjct: 123 LKCKDCIDGFVGCLEKDGSNVFIDWVKNNQIKVILVLGICTDICVLDFVCSTLSARNRGF 182

Query: 181 LSPLEDVIVYSGGCATYDLPVAVAKTLGDAIAHPQELMHHIGLYIARGRGAKVVSK 236
           L PL+DVI+YSGGCAT+DLPV+VAK LGDAIAHPQ LMHHIGLY+ARGRGAKVVS+
Sbjct: 183 LPPLKDVIIYSGGCATFDLPVSVAKNLGDAIAHPQALMHHIGLYLARGRGAKVVSE 238

[Close]

BLAST of Chy2G030270 vs. NCBI nr
Match: KAG6594908.1 (Nicotinamidase 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7026874.1 Nicotinamidase 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 442 bits (1136), Expect = 3.39e-155
Identity = 209/236 (88.56%), Postives = 225/236 (95.34%), Query Frame = 0


Query: 1   MVLEVMELLKEHLPLTQEPFVLSGDLNIGLVLVDVVNGFCTVGAGNLAPKQHNEQISQMV 60
           MV EV+ELLKE+LP+TQEPFVLSGD+N GLVLVDVVNGFCTVGAGNLAPKQH+EQISQMV
Sbjct: 1   MVSEVVELLKENLPVTQEPFVLSGDVNTGLVLVDVVNGFCTVGAGNLAPKQHDEQISQMV 60

Query: 61  EESARLARVFCEKKWPIFAFCDSHHPDIPEPPYPPHCIAGTDESKLVPALQWLENEANVT 120
           EESAR ARVFCEKKWPIFAF DSH PDIPEPPYPPHCIAGTDE+KLVPALQWLENE NVT
Sbjct: 61  EESARFARVFCEKKWPIFAFLDSHRPDIPEPPYPPHCIAGTDEAKLVPALQWLENETNVT 120

Query: 121 LRCKDCIDGFLGCLEKDGSNIFIDWVKKNQIKGILVLGICTDICVLDFVCSTLSARNRGF 180
           L+CKDCIDGF+GCLEKDGSN+F+DWVK NQIK ILVLGICTDICVLDFVCSTLSARNRGF
Sbjct: 121 LKCKDCIDGFVGCLEKDGSNVFVDWVKNNQIKVILVLGICTDICVLDFVCSTLSARNRGF 180

Query: 181 LSPLEDVIVYSGGCATYDLPVAVAKTLGDAIAHPQELMHHIGLYIARGRGAKVVSK 236
           L PL+DVI+YSGGCAT+DLPV+VAK LGDAIAHPQ LMHHIGLY+ARGRGAKVVS+
Sbjct: 181 LPPLKDVIIYSGGCATFDLPVSVAKNLGDAIAHPQALMHHIGLYLARGRGAKVVSE 236

[Close]

BLAST of Chy2G030270 vs. TAIR 10
Match: AT2G22570.1 (nicotinamidase 1 )

HSP 1 Score: 352.8 bits (904), Expect = 2.4e-97
Identity = 158/233 (67.81%), Postives = 200/233 (85.84%), Query Frame = 0


Query: 5   VMELLKEHLPL-TQEPFVLSGDLNIGLVLVDVVNGFCTVGAGNLAPKQHNEQISQMVEES 64
           + + LK+ +P+  +EP +L+ D ++GLV+VDVVNGFCT+G+GN+AP +HNEQIS+MVEES
Sbjct: 7   IFDQLKKQIPVDEEEPLILNRDSSVGLVIVDVVNGFCTIGSGNMAPTKHNEQISKMVEES 66

Query: 65  ARLARVFCEKKWPIFAFCDSHHPDIPEPPYPPHCIAGTDESKLVPALQWLENEANVTLRC 124
           A+LAR FC++KWP+ AF DSHHPDIPE PYPPHCI GT+ES+LVPAL+WLE+E   TLR 
Sbjct: 67  AKLAREFCDRKWPVLAFIDSHHPDIPERPYPPHCIIGTEESELVPALKWLESEDCATLRR 126

Query: 125 KDCIDGFLGCLEKDGSNIFIDWVKKNQIKGILVLGICTDICVLDFVCSTLSARNRGFLSP 184
           KDCI+GF+G +E DGSN+F+DWVK+ QIK I+V+GICTDICV DFV + LSARN G LSP
Sbjct: 127 KDCINGFVGSMESDGSNVFVDWVKEKQIKVIVVVGICTDICVFDFVATALSARNHGVLSP 186

Query: 185 LEDVIVYSGGCATYDLPVAVAKTLGDAIAHPQELMHHIGLYIARGRGAKVVSK 237
           +EDV+VYS GCAT+DLP+ VAK +  A AHPQELMHH+GLY+A+GRGA+VVSK
Sbjct: 187 VEDVVVYSRGCATFDLPLHVAKDIKGAQAHPQELMHHVGLYMAKGRGAQVVSK 239

[Close]

BLAST of Chy2G030270 vs. TAIR 10
Match: AT2G22570.2 (nicotinamidase 1 )

HSP 1 Score: 227.3 bits (578), Expect = 1.5e-59
Identity = 98/149 (65.77%), Postives = 128/149 (85.91%), Query Frame = 0


Query: 5   VMELLKEHLPL-TQEPFVLSGDLNIGLVLVDVVNGFCTVGAGNLAPKQHNEQISQMVEES 64
           + + LK+ +P+  +EP +L+ D ++GLV+VDVVNGFCT+G+GN+AP +HNEQIS+MVEES
Sbjct: 7   IFDQLKKQIPVDEEEPLILNRDSSVGLVIVDVVNGFCTIGSGNMAPTKHNEQISKMVEES 66

Query: 65  ARLARVFCEKKWPIFAFCDSHHPDIPEPPYPPHCIAGTDESKLVPALQWLENEANVTLRC 124
           A+LAR FC++KWP+ AF DSHHPDIPE PYPPHCI GT+ES+LVPAL+WLE+E   TLR 
Sbjct: 67  AKLAREFCDRKWPVLAFIDSHHPDIPERPYPPHCIIGTEESELVPALKWLESEDCATLRR 126

Query: 125 KDCIDGFLGCLEKDGSNIFIDWVKKNQIK 153
           KDCI+GF+G +E DGSN+F+DWVK+ QIK
Sbjct: 127 KDCINGFVGSMESDGSNVFVDWVKEKQIK 155

The following BLAST results are available for this feature:

BLAST of Chy2G030270 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8S8F9	3.4e-96	67.81	Nicotinamidase 1 OS=Arabidopsis thaliana OX=3702 GN=NIC1 PE=1 SV=1	[more]
O32091	2.3e-04	34.62	Uncharacterized isochorismatase family protein PncA OS=Bacillus subtilis (strain...	[more]

BLAST of Chy2G030270 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KG59	5.5e-134	98.73	Catalytic OS=Cucumis sativus OX=3659 GN=Csa_6G498960 PE=3 SV=1	[more]
A0A5D3CLP4	3.7e-130	95.76	Nicotinamidase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G003...	[more]
A0A1S3B1Y8	3.7e-130	95.76	nicotinamidase 1 OS=Cucumis melo OX=3656 GN=LOC103484929 PE=3 SV=1	[more]
A0A6J1KQH8	2.6e-123	89.41	nicotinamidase 1 OS=Cucurbita maxima OX=3661 GN=LOC111496727 PE=3 SV=1	[more]
A0A6J1HG67	3.7e-122	88.56	nicotinamidase 1 OS=Cucurbita moschata OX=3662 GN=LOC111463275 PE=3 SV=1	[more]

BLAST of Chy2G030270 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004143443.1	1.37e-170	98.73	nicotinamidase 1 [Cucumis sativus] >KGN48700.1 hypothetical protein Csa_003282 [...	[more]
XP_008440534.1	1.47e-165	95.76	PREDICTED: nicotinamidase 1 [Cucumis melo] >KAA0036347.1 nicotinamidase 1 [Cucum...	[more]
XP_038881297.1	1.51e-158	91.53	nicotinamidase 1 [Benincasa hispida]	[more]
XP_023002980.1	1.55e-156	89.41	nicotinamidase 1 [Cucurbita maxima]	[more]
KAG6594908.1	3.39e-155	88.56	Nicotinamidase 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7026874.1 ...	[more]

BLAST of Chy2G030270 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G22570.1	2.4e-97	67.81	nicotinamidase 1	[more]
AT2G22570.2	1.5e-59	65.77	nicotinamidase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000868	Isochorismatase-like	PFAM	PF00857	Isochorismatase	coord: 30..199 e-value: 1.0E-29 score: 104.0
IPR036380	Isochorismatase-like superfamily	GENE3D	3.40.50.850		coord: 29..222 e-value: 7.1E-26 score: 93.4
IPR036380	Isochorismatase-like superfamily	SUPERFAMILY	52499	Isochorismatase-like hydrolases	coord: 30..235
IPR044717	Nicotinamidase 1	PANTHER	PTHR47297	FAMILY NOT NAMED	coord: 3..236
None	No IPR available	CDD	cd00431	cysteine_hydrolases	coord: 30..199 e-value: 1.09959E-26 score: 100.036

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy2G030270.1	Chy2G030270.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019365	pyridine nucleotide salvage
molecular_function	GO:0008936	nicotinamidase activity