Chy2G029510 (gene) Cucumber (hystrix) v1

Overview
NameChy2G029510
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
Descriptioncytochrome P450 CYP736A12-like
LocationchrH02: 4156667 .. 4157862 (-)
RNA-Seq ExpressionChy2G029510
SyntenyChy2G029510
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTTGATTTTTGCCATTGTTTCTACATTAGCTCTTCTTGCTCTCACTCTTCTCTTCAAATTCTACCCCTTCAAAGCCCAAAAATTGCCTCCTGGTCCTATAGGATTTCCCTTTGTTGGTAGTCTCCATTTATTAGGAAAGCTCCCTCATAGAGATTTTCATAGATTATCCCAAAAATATGGACCCATTATGCACATAAAATTAGGCCTTGTTCCCACAATCATAGTCTCTTCTCCTAAAGCTGCTGAACTCTTCCTCAAAACTCATGACCTTGTTTTTGCAAGCAGGCCCCTTATAGAAGCCTCAAAGCAAATGAATTATGGGCAAAAGAACTTAGTTTTTGCCCCATATGGCCCTTATTGGCGCAACATGCGTAAAATGTGCACTCTTGAATTGCTTAGTAACCTTAAAATCAACTCTTTCATGCCTATGAGAAAACACGAGCTTGGTTTGTTGATTGAGTACTTAAAAGAAGCTGCTCAAAACAAAGCTGTTGTGAATCTGAGTGCTAAAGTTACGTCACTTACAACTGACCTTATTTGTTTGATGGCATTTGGGAAGAAATATGGAGATGAAGAGATTGATGAAAGGGGATTTAAAGCTGTGCTACAAGAAGGTTCACAATTAGCTGCTACTCCTAATTTGGGGGATTTTTTTCCTTTTATTGCAAGGTTTGATGTTCAAAGATTGAATAATCGAATGCAATGTGTTCATAAAGTGTTGGATGAGTTTCTTGAGAGGATTGTTAATGAACATCTTGAGGCTAAGGGTGACAAGAAGACTAAAGATCTTGTGGATGTTATGTTGGAGCTTATGAATTTCCAAGAAGAAACTGATTATCAGATTGATCGCTCTGCTATTAAAGCTATAATGCTGGTATGGATCCCTGCTATATATATACATATATTCATTCATTTTACTACACATGTACTCCCTAACTTAAAAAAAAATGTTATGATAGTACGTGAAATAATTCTAATTTTGTGTTCAACAACTCCGTAATATATTTGAAATTTTAAAAGATTAGAACTTAATTAGTATAATGTATATTGAATTGGTGTGCTCTCTTAATTTAATCTACTTTGTTTTATCGTGTTTAATTAAACGATGGTTTGGTTACTTAGTTGGATAAAAGAAGAGAACAAATTTCAGGTAGTTTCGTTAAATATTATATTGTAGGTAAAGCAGAGTAG

mRNA sequence

ATGGCTTTGATTTTTGCCATTGTTTCTACATTAGCTCTTCTTGCTCTCACTCTTCTCTTCAAATTCTACCCCTTCAAAGCCCAAAAATTGCCTCCTGGTCCTATAGGATTTCCCTTTGTTGGTAGTCTCCATTTATTAGGAAAGCTCCCTCATAGAGATTTTCATAGATTATCCCAAAAATATGGACCCATTATGCACATAAAATTAGGCCTTGTTCCCACAATCATAGTCTCTTCTCCTAAAGCTGCTGAACTCTTCCTCAAAACTCATGACCTTGTTTTTGCAAGCAGGCCCCTTATAGAAGCCTCAAAGCAAATGAATTATGGGCAAAAGAACTTAGTTTTTGCCCCATATGGCCCTTATTGGCGCAACATGCGTAAAATGTGCACTCTTGAATTGCTTAGTAACCTTAAAATCAACTCTTTCATGCCTATGAGAAAACACGAGCTTGGTTTGTTGATTGAGTACTTAAAAGAAGCTGCTCAAAACAAAGCTGTTGTGAATCTGAGTGCTAAAGTTACGTCACTTACAACTGACCTTATTTGTTTGATGGCATTTGGGAAGAAATATGGAGATGAAGAGATTGATGAAAGGGGATTTAAAGCTGTGCTACAAGAAGGTTCACAATTAGCTGCTACTCCTAATTTGGGGGATTTTTTTCCTTTTATTGCAAGGTTTGATGTTCAAAGATTGAATAATCGAATGCAATGTGTTCATAAAGTGTTGGATGAGTTTCTTGAGAGGATTGTTAATGAACATCTTGAGGCTAAGGGTGACAAGAAGACTAAAGATCTTGTGGATGTTATGTTGGAGCTTATGAATTTCCAAGAAGAAACTGATTATCAGATTGATCGCTCTGCTATTAAAGCTATAATGCTGTTGGATAAAAGAAGAGAACAAATTTCAGGTAGTTTCGTTAAATATTATATTGTAGGTAAAGCAGAGTAG

Coding sequence (CDS)

ATGGCTTTGATTTTTGCCATTGTTTCTACATTAGCTCTTCTTGCTCTCACTCTTCTCTTCAAATTCTACCCCTTCAAAGCCCAAAAATTGCCTCCTGGTCCTATAGGATTTCCCTTTGTTGGTAGTCTCCATTTATTAGGAAAGCTCCCTCATAGAGATTTTCATAGATTATCCCAAAAATATGGACCCATTATGCACATAAAATTAGGCCTTGTTCCCACAATCATAGTCTCTTCTCCTAAAGCTGCTGAACTCTTCCTCAAAACTCATGACCTTGTTTTTGCAAGCAGGCCCCTTATAGAAGCCTCAAAGCAAATGAATTATGGGCAAAAGAACTTAGTTTTTGCCCCATATGGCCCTTATTGGCGCAACATGCGTAAAATGTGCACTCTTGAATTGCTTAGTAACCTTAAAATCAACTCTTTCATGCCTATGAGAAAACACGAGCTTGGTTTGTTGATTGAGTACTTAAAAGAAGCTGCTCAAAACAAAGCTGTTGTGAATCTGAGTGCTAAAGTTACGTCACTTACAACTGACCTTATTTGTTTGATGGCATTTGGGAAGAAATATGGAGATGAAGAGATTGATGAAAGGGGATTTAAAGCTGTGCTACAAGAAGGTTCACAATTAGCTGCTACTCCTAATTTGGGGGATTTTTTTCCTTTTATTGCAAGGTTTGATGTTCAAAGATTGAATAATCGAATGCAATGTGTTCATAAAGTGTTGGATGAGTTTCTTGAGAGGATTGTTAATGAACATCTTGAGGCTAAGGGTGACAAGAAGACTAAAGATCTTGTGGATGTTATGTTGGAGCTTATGAATTTCCAAGAAGAAACTGATTATCAGATTGATCGCTCTGCTATTAAAGCTATAATGCTGTTGGATAAAAGAAGAGAACAAATTTCAGGTAGTTTCGTTAAATATTATATTGTAGGTAAAGCAGAGTAG

Protein sequence

MALIFAIVSTLALLALTLLFKFYPFKAQKLPPGPIGFPFVGSLHLLGKLPHRDFHRLSQKYGPIMHIKLGLVPTIIVSSPKAAELFLKTHDLVFASRPLIEASKQMNYGQKNLVFAPYGPYWRNMRKMCTLELLSNLKINSFMPMRKHELGLLIEYLKEAAQNKAVVNLSAKVTSLTTDLICLMAFGKKYGDEEIDERGFKAVLQEGSQLAATPNLGDFFPFIARFDVQRLNNRMQCVHKVLDEFLERIVNEHLEAKGDKKTKDLVDVMLELMNFQEETDYQIDRSAIKAIMLLDKRREQISGSFVKYYIVGKAE*
Homology
BLAST of Chy2G029510 vs. ExPASy Swiss-Prot
Match: A0A068Q5V6 (Cytochrome P450 71AU50 OS=Prunus mume OX=102107 GN=CYP71AU50 PE=2 SV=1)

HSP 1 Score: 323.6 bits (828), Expect = 2.6e-87
Identity = 163/289 (56.40%), Postives = 217/289 (75.09%), Query Frame = 0

Query: 7   IVSTLALLALT-LLFKFYPFKAQKLPPGPIGFPFVGSLHLLGKLPHRDFHRLSQKYGPIM 66
           I +T+ LLAL  +L  ++  K ++LPPGP GFP  GSLHLLG+ P++D HRL++KYG IM
Sbjct: 4   IWATIGLLALVHILQAWWKNKKKRLPPGPRGFPIFGSLHLLGEFPNKDLHRLARKYGDIM 63

Query: 67  HIKLGLVPTIIVSSPKAAELFLKTHDLVFASRPLIEASKQMNYGQKNLVFAPYGPYWRNM 126
           +++LGL+PTI++SSP+AAELFLKTHDLVFASRP  E SK +++GQKNL+F+ YG YWR+ 
Sbjct: 64  YMRLGLMPTIVISSPEAAELFLKTHDLVFASRPPHEGSKHISFGQKNLIFSEYGAYWRDT 123

Query: 127 RKMCTLELLSNLKINSFMPMRKHELGLLIEYLKEAAQNKAV-VNLSAKVTSLTTDLICLM 186
           RKMCT+ELLSN KINSF  MR+ E+ L +E ++ AA N+ V V+LS KV+SL+ D+ C M
Sbjct: 124 RKMCTIELLSNHKINSFKSMRREEVSLCVESIRAAANNRGVAVDLSDKVSSLSVDMSCRM 183

Query: 187 AFGKKYGDEEIDERGFKAVLQEGSQLAATPNLGDFFPFIARFDVQRLNNRMQCVHKVLDE 246
             GKKY DEE DERGFK+V++E  QLA+ PNLGD+  FIA  D+Q    RM+ V+K  D 
Sbjct: 184 VLGKKYRDEEFDERGFKSVVREAIQLASAPNLGDYIRFIAPLDLQGFTKRMKSVNKAFDN 243

Query: 247 FLERIVNEHLEAKGDKKTKDLVDVMLELMNFQEETDYQIDRSAIKAIML 294
             E+I+ EHL+    ++T D VDVM+  M   EE++Y+I+R  IKAIML
Sbjct: 244 LFEKIIEEHLQPNDGERTMDFVDVMVGFMG-SEESEYRIERPHIKAIML 291

BLAST of Chy2G029510 vs. ExPASy Swiss-Prot
Match: H2DH18 (Cytochrome P450 CYP736A12 OS=Panax ginseng OX=4054 PE=2 SV=1)

HSP 1 Score: 235.0 bits (598), Expect = 1.2e-60
Identity = 131/297 (44.11%), Postives = 185/297 (62.29%), Query Frame = 0

Query: 3   LIFAIVSTLALLALTLLFKFYPFKA-QKLPPGPIGFPFVGSLHLLGKLPHRDFHRLSQKY 62
           L+F ++  L+   L ++    P K   KLPPGP G P +GSLH LG LPHR    L++KY
Sbjct: 8   LLFVLLGALSWWILPII---SPLKRHHKLPPGPRGLPIIGSLHTLGALPHRTLQTLAKKY 67

Query: 63  GPIMHIKLGLVPTIIVSSPKAAELFLKTHDLVFASRPLIEASKQMNYGQKNLVFAPYGPY 122
           GPIM ++LG VPTI+VSSP+AAELFLKTHD +FASRP ++A++ M+YG   + F  YGP+
Sbjct: 68  GPIMSMRLGSVPTIVVSSPQAAELFLKTHDNIFASRPKLQAAEYMSYGTMGMSFTAYGPH 127

Query: 123 WRNMRKMCTLELLSNLKINSFMPMRKHELGLLIEYLKEAAQNKAVVNLSAKVTSLTTDLI 182
           WRN+RK   LELL+  KINSF+ MR+ ELG +++ +KEA+    VV+LSAKV ++  ++ 
Sbjct: 128 WRNIRKFVVLELLTPAKINSFVGMRREELGTVVKSIKEASAANEVVDLSAKVANIIENMT 187

Query: 183 CLMAFGKKYGDEEIDERGFKAVLQEGSQLAATPNLGDFFPFIARFDVQRLNNRMQCVHKV 242
             +  G+     + D    K ++ E   LA   N+ DF PF+   D+Q L  + +   K 
Sbjct: 188 YRLLLGR----TKDDRYDLKGIMNEALTLAGRFNIADFVPFLGPLDIQGLTRQFKDTGKR 247

Query: 243 LDEFLERIVNEHLE-AKGDKKTKDLVDVMLEL----MNFQEETDYQIDRSAIKAIML 294
           LD+ LE I++EH + +     + D +D ML L     N  +E    IDRS IKAIM+
Sbjct: 248 LDKILEFIIDEHEQNSSNGNASGDFIDDMLSLKNKPSNTHDELSKVIDRSVIKAIMI 297

BLAST of Chy2G029510 vs. ExPASy Swiss-Prot
Match: P24465 (Cytochrome P450 71A1 OS=Persea americana OX=3435 GN=CYP71A1 PE=1 SV=2)

HSP 1 Score: 198.7 bits (504), Expect = 9.5e-50
Identity = 114/299 (38.13%), Postives = 177/299 (59.20%), Query Frame = 0

Query: 6   AIVSTLALLALTLLFKFYPF-----KAQKLPPGPIGFPFVGSLHLLGKLPHRDFHRLSQK 65
           AI+ +L  LA+ L F          K   LPP P   P +G+LH LG LPHR    L+ +
Sbjct: 2   AILVSLLFLAIALTFFLLKLNEKREKKPNLPPSPPNLPIIGNLHQLGNLPHRSLRSLANE 61

Query: 66  YGPIMHIKLGLVPTIIVSSPKAAELFLKTHDLVFASRPLIEASKQMNYGQKNLVFAPYGP 125
            GP++ + LG +PT+IVS+ + AE  LKTHDL+FASRP   A++++ Y   ++ F+PYG 
Sbjct: 62  LGPLILLHLGHIPTLIVSTAEIAEEILKTHDLIFASRPSTTAARRIFYDCTDVAFSPYGE 121

Query: 126 YWRNMRKMCTLELLSNLKINSFMPMRKHELGLLIEYLKEAAQNKAVVNLSAKVTSLTTDL 185
           YWR +RK+C LELLS  ++NS+  +R+ E+GL++E + ++      VNLS  +  L++  
Sbjct: 122 YWRQVRKICVLELLSIKRVNSYRSIREEEVGLMMERISQSCSTGEAVNLSELLLLLSSGT 181

Query: 186 ICLMAFGKKYGDEEIDERGFKAVLQEGSQLAATPNLGDFFPFIARFDV-QRLNNRMQCVH 245
           I  +AFGKKY  EE  +  F  +  E + L     +GD+FP  A  DV   ++ R++  H
Sbjct: 182 ITRVAFGKKYEGEEERKNKFADLATELTTLMGAFFVGDYFPSFAWVDVLTGMDARLKRNH 241

Query: 246 KVLDEFLERIVNEHL---EAKGDK--KTKDLVDVMLELMNFQEETDYQIDRSAIKAIML 294
             LD F++ ++++HL   +A G    + KDLVDV+L L          ++R+ +KA++L
Sbjct: 242 GELDAFVDHVIDDHLLSRKANGSDGVEQKDLVDVLLHLQK-DSSLGVHLNRNNLKAVIL 299

BLAST of Chy2G029510 vs. ExPASy Swiss-Prot
Match: O65788 (Cytochrome P450 71B2 OS=Arabidopsis thaliana OX=3702 GN=CYP71B2 PE=2 SV=2)

HSP 1 Score: 196.8 bits (499), Expect = 3.6e-49
Identity = 109/298 (36.58%), Postives = 188/298 (63.09%), Query Frame = 0

Query: 3   LIFAIVSTLALLALTLLFKFYPFKAQKLPPGPIGFPFVGSLHLLGKLPHRDFHRLSQKYG 62
           L F +VS L +++ ++  K        LPP P   P +G+LH L  LPHR FH+LS KYG
Sbjct: 5   LCFFLVSLLTIVS-SIFLKQNKTSKFNLPPSPSSLPIIGNLHHLAGLPHRCFHKLSIKYG 64

Query: 63  PIMHIKLGLVPTIIVSSPKAAELFLKTHDLVFASRPLIEASKQMNYGQKNLVFAPYGPYW 122
           P++ ++LG VP +++SS +AAE  LKT+DL   SRP    S +++YG K++ FAPYG YW
Sbjct: 65  PLVFLRLGSVPVVVISSSEAAEAVLKTNDLECCSRPKTVGSGKLSYGFKDITFAPYGEYW 124

Query: 123 RNMRKMCTLELLSNLKINSFMPMRKHELGLLIEYLKEAAQNKAVVNLSAKVTSLTTDLIC 182
           R +RK+  +EL S+ K+ SF  +R+ E+  +++ + E+A  ++ V+LS    SLT  +IC
Sbjct: 125 REVRKLAVIELFSSKKVQSFRYIREEEVDFVVKKVSESALKQSPVDLSKTFFSLTASIIC 184

Query: 183 LMAFGKKYGDE--EIDERGFKAVLQEGSQLAATPNLGDFFP-FIARFDVQRLNNRMQCVH 242
            +A G+ + +    ID+   + ++ E ++   T    DFFP  + RF V  L  R + ++
Sbjct: 185 RVALGQNFNESGFVIDQDRIEELVTESAEALGTFTFSDFFPGGLGRF-VDWLFQRHKKIN 244

Query: 243 KV---LDEFLERIVNEHLEAKGDKKTKDLVDVMLELMNFQEETD-YQIDRSAIKAIML 294
           KV   LD F + ++++HL+ +G +K +D+V ++L++++ QE++D ++++   +KAI++
Sbjct: 245 KVFKELDAFYQHVIDDHLKPEG-RKNQDIVTLILDMIDKQEDSDSFKLNMDNLKAIVM 299

BLAST of Chy2G029510 vs. ExPASy Swiss-Prot
Match: A0A068Q6L2 (Cytochrome P450 736A117 OS=Prunus mume OX=102107 GN=CYP736A117 PE=2 SV=1)

HSP 1 Score: 196.1 bits (497), Expect = 6.2e-49
Identity = 112/297 (37.71%), Postives = 168/297 (56.57%), Query Frame = 0

Query: 10  TLALLALTLLFKFY-----PFKAQKLPPGPIGFPFVGSLHLLGKLPHRDFHRLSQKYGPI 69
           TL  + L LL+ +Y       +    PP P   P +G+LH +G  PHR    LSQ++GP+
Sbjct: 24  TLLAIFLVLLYTWYSSTKTTTQKSTQPPSPPKLPIIGNLHQIGSYPHRSLQALSQRHGPL 83

Query: 70  MHIKLGLVPTIIVSSPKAAELFLKTHDLVFASRPLIEASKQMNYGQKNLVFAPYGPYWRN 129
           M +  G VP ++VSS +AA   LKTHDL F+ RP     +++ Y  K++  APYG YWR 
Sbjct: 84  MLLHFGSVPVLVVSSAEAAREILKTHDLTFSDRPKSTIFEKLLYNYKDVASAPYGEYWRQ 143

Query: 130 MRKMCTLELLSNLKINSFMPMRKHELGLLIEYLKEAAQNKAVVNLSAKVTSLTTDLICLM 189
           +R +C L LLSN ++ SF  +R+ E   +I  +K    + +V+NLS     LT D++C +
Sbjct: 144 VRSICVLNLLSNRRVRSFRSVREEETKSMIRNIK--GSSSSVLNLSEMFVRLTNDVVCKV 203

Query: 190 AFGKKYGDEEIDERG--FKAVLQEGSQLAATPNLGDFFPFIARFD-VQRLNNRMQCVHKV 249
           A G+KY D E  E G  FK +L E   L  T N+GD+ P+++    V  L  ++  V K 
Sbjct: 204 ALGRKYSDGEGGESGRMFKEILGEFGDLLGTVNIGDYVPWLSWLSHVNGLGAKLDKVAKQ 263

Query: 250 LDEFLERIVNEHL---EAKGDKKTKDLVDVMLELMNFQEETD--YQIDRSAIKAIML 294
           LD+F++ +V EH+      GD   KD +D++L +   Q+ET     ID  ++K I+L
Sbjct: 264 LDDFIDTVVQEHMNHSSRSGDDDQKDFLDILLAI---QKETSAGIPIDGVSVKGIIL 315

BLAST of Chy2G029510 vs. ExPASy TrEMBL
Match: A0A0A0KJ01 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G501340 PE=3 SV=1)

HSP 1 Score: 572.0 bits (1473), Expect = 1.5e-159
Identity = 291/312 (93.27%), Postives = 298/312 (95.51%), Query Frame = 0

Query: 1   MALIFAIVSTLALLALTLLFKFYPFKAQKLPPGPIGFPFVGSLHLLGKLPHRDFHRLSQK 60
           MALIFAI+STLALLALTLLFKFYPFKAQKLPPGPIGFPFVGSLHLLGKLPHRDFH LSQK
Sbjct: 1   MALIFAIISTLALLALTLLFKFYPFKAQKLPPGPIGFPFVGSLHLLGKLPHRDFHILSQK 60

Query: 61  YGPIMHIKLGLVPTIIVSSPKAAELFLKTHDLVFASRPLIEASKQMNYGQKNLVFAPYGP 120
           YGPIMHIKLGLVPTIIVSSPKAAELFLKTHDLVFASRPL+EASKQMNYGQKNLVFAPYGP
Sbjct: 61  YGPIMHIKLGLVPTIIVSSPKAAELFLKTHDLVFASRPLLEASKQMNYGQKNLVFAPYGP 120

Query: 121 YWRNMRKMCTLELLSNLKINSFMPMRKHELGLLIEYLKEAAQNKAVVNLSAKVTSLTTDL 180
           YWRNMRKMCTLELLSNLKINSFMPMRKHELGLLIEYLKEAA NKAVVNLSAKVTSLTTDL
Sbjct: 121 YWRNMRKMCTLELLSNLKINSFMPMRKHELGLLIEYLKEAAHNKAVVNLSAKVTSLTTDL 180

Query: 181 ICLMAFGKKYGDEEIDERGFKAVLQEGSQLAATPNLGDFFPFIARFDVQRLNNRMQCVHK 240
           ICLMAFGKKYGDEEIDERGFKA +QEGSQLAATPNLGDFFPFIARFDVQRLNNRMQCVHK
Sbjct: 181 ICLMAFGKKYGDEEIDERGFKATIQEGSQLAATPNLGDFFPFIARFDVQRLNNRMQCVHK 240

Query: 241 VLDEFLERIVNEHLEAKGDKKTKDLVDVMLELMNFQEETDYQIDRSAIKAIMLLDKRREQ 300
           VLD FLERIVNEHLEAKGDKKTKDLVDVMLELMNFQEETDYQIDRSAIKAIML  + R  
Sbjct: 241 VLDGFLERIVNEHLEAKGDKKTKDLVDVMLELMNFQEETDYQIDRSAIKAIMLEKQSRND 300

Query: 301 I---SGSFVKYY 310
           I   S SF+ ++
Sbjct: 301 ICKSSKSFLSFF 312

BLAST of Chy2G029510 vs. ExPASy TrEMBL
Match: A0A6J1BU49 (cytochrome P450 CYP736A12-like OS=Momordica charantia OX=3673 GN=LOC111005380 PE=3 SV=1)

HSP 1 Score: 430.3 bits (1105), Expect = 7.2e-117
Identity = 223/293 (76.11%), Postives = 250/293 (85.32%), Query Frame = 0

Query: 1   MALIFAIVSTLALLALTLLFKFYPFKAQKLPPGPIGFPFVGSLHLLGKLPHRDFHRLSQK 60
           MA I+AI  +  LLAL LLFK + FK + LPPGP GF   GSLHLLGKLPHRD  +LSQK
Sbjct: 1   MAWIWAI--SPLLLALALLFKPWLFKPKNLPPGPKGFSIFGSLHLLGKLPHRDLQKLSQK 60

Query: 61  YGPIMHIKLGLVPTIIVSSPKAAELFLKTHDLVFASRPLIEASKQMNYGQKNLVFAPYGP 120
           YGPIMH+KLGLVPTIIVSSP AAELFLKTHDLVFASRPL EASKQMNY QKNLVFAPYG 
Sbjct: 61  YGPIMHMKLGLVPTIIVSSPSAAELFLKTHDLVFASRPLSEASKQMNYCQKNLVFAPYGS 120

Query: 121 YWRNMRKMCTLELLSNLKINSFMPMRKHELGLLIEYLKEAAQNKAVVNLSAKVTSLTTDL 180
           YWRNMRKMCTLELLS+ KINSF  MRK EL LLI++L+EAA N   VNLS+KVTSLTTD+
Sbjct: 121 YWRNMRKMCTLELLSSRKINSFKSMRKRELALLIDHLREAADNGVAVNLSSKVTSLTTDM 180

Query: 181 ICLMAFGKKYGDEEIDERGFKAVLQEGSQLAATPNLGDFFPFIARFDVQRLNNRMQCVHK 240
            CLM FG KY D++ DERGFKAV+QEGSQLAATPNLGDFFPFIA  D+QRLN +M+ VHK
Sbjct: 181 TCLMVFGMKYEDKDFDERGFKAVVQEGSQLAATPNLGDFFPFIAGLDLQRLNKQMKSVHK 240

Query: 241 VLDEFLERIVNEHLEAKGDKKTKDLVDVMLELMNFQEETDYQIDRSAIKAIML 294
            LD+FLERIVN+HLE+K ++KTKD VDVML++M+FQ ET+YQIDR AIKAIML
Sbjct: 241 ALDDFLERIVNDHLESKDEEKTKDFVDVMLDVMSFQ-ETEYQIDRPAIKAIML 290

BLAST of Chy2G029510 vs. ExPASy TrEMBL
Match: A0A5A7SY99 (Cytochrome P450 CYP736A12-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G002460 PE=3 SV=1)

HSP 1 Score: 420.6 bits (1080), Expect = 5.7e-114
Identity = 218/297 (73.40%), Postives = 246/297 (82.83%), Query Frame = 0

Query: 1   MALIFAIVSTLALLALTL----LFKFYPFKAQKLPPGPIGFPFVGSLHLLGKLPHRDFHR 60
           MALI  IV    LLAL +    LFK    K +K PPGP G P  G LHLLGKLPHR+ H+
Sbjct: 1   MALILVIVVIALLLALPISHSWLFK-TRIKPKKFPPGPKGIPIFGCLHLLGKLPHRNLHK 60

Query: 61  LSQKYGPIMHIKLGLVPTIIVSSPKAAELFLKTHDLVFASRPLIEASKQMNYGQKNLVFA 120
           LSQKYGPIM +KLGLVPTIIVSSP+AA+LFLKTHDL+FASRP  +ASK ++Y Q+NLVFA
Sbjct: 61  LSQKYGPIMSMKLGLVPTIIVSSPQAAKLFLKTHDLIFASRPSSQASKHISYQQQNLVFA 120

Query: 121 PYGPYWRNMRKMCTLELLSNLKINSFMPMRKHELGLLIEYLKEAAQNKAVVNLSAKVTSL 180
           PYGPYWRNMRKMCTLELLSN KINSFMP+RKHELGLLIEY KEAA NK VVNLSAKVTSL
Sbjct: 121 PYGPYWRNMRKMCTLELLSNHKINSFMPIRKHELGLLIEYFKEAAHNKTVVNLSAKVTSL 180

Query: 181 TTDLICLMAFGKKYGDEEIDERGFKAVLQEGSQLAATPNLGDFFPFIARFDVQRLNNRMQ 240
           TTD+ICLMAFGKKYGDE+ DERGFKAV+QEG QLAA PNLGDF P IA  D+Q    +M+
Sbjct: 181 TTDIICLMAFGKKYGDEDFDERGFKAVIQEGMQLAAAPNLGDFIPAIAWLDLQGFTRKMK 240

Query: 241 CVHKVLDEFLERIVNEHLEAKGDKKTKDLVDVMLELMNFQEETDYQIDRSAIKAIML 294
            VHKV DEFLE+I+NEHL A+G KKT+D VDVML+L++ Q +T+YQIDRS IKAIML
Sbjct: 241 SVHKVYDEFLEKIINEHLVARGGKKTRDFVDVMLDLIDSQ-QTEYQIDRSTIKAIML 295

BLAST of Chy2G029510 vs. ExPASy TrEMBL
Match: A0A1S3B175 (cytochrome P450 CYP736A12-like OS=Cucumis melo OX=3656 GN=LOC103485010 PE=3 SV=1)

HSP 1 Score: 420.6 bits (1080), Expect = 5.7e-114
Identity = 218/297 (73.40%), Postives = 246/297 (82.83%), Query Frame = 0

Query: 1   MALIFAIVSTLALLALTL----LFKFYPFKAQKLPPGPIGFPFVGSLHLLGKLPHRDFHR 60
           MALI  IV    LLAL +    LFK    K +K PPGP G P  G LHLLGKLPHR+ H+
Sbjct: 1   MALILVIVVIALLLALPISHSWLFK-TRIKPKKFPPGPKGIPIFGCLHLLGKLPHRNLHK 60

Query: 61  LSQKYGPIMHIKLGLVPTIIVSSPKAAELFLKTHDLVFASRPLIEASKQMNYGQKNLVFA 120
           LSQKYGPIM +KLGLVPTIIVSSP+AA+LFLKTHDL+FASRP  +ASK ++Y Q+NLVFA
Sbjct: 61  LSQKYGPIMSMKLGLVPTIIVSSPQAAKLFLKTHDLIFASRPSSQASKHISYQQQNLVFA 120

Query: 121 PYGPYWRNMRKMCTLELLSNLKINSFMPMRKHELGLLIEYLKEAAQNKAVVNLSAKVTSL 180
           PYGPYWRNMRKMCTLELLSN KINSFMP+RKHELGLLIEY KEAA NK VVNLSAKVTSL
Sbjct: 121 PYGPYWRNMRKMCTLELLSNHKINSFMPIRKHELGLLIEYFKEAAHNKTVVNLSAKVTSL 180

Query: 181 TTDLICLMAFGKKYGDEEIDERGFKAVLQEGSQLAATPNLGDFFPFIARFDVQRLNNRMQ 240
           TTD+ICLMAFGKKYGDE+ DERGFKAV+QEG QLAA PNLGDF P IA  D+Q    +M+
Sbjct: 181 TTDIICLMAFGKKYGDEDFDERGFKAVIQEGMQLAAAPNLGDFIPAIAWLDLQGFTRKMK 240

Query: 241 CVHKVLDEFLERIVNEHLEAKGDKKTKDLVDVMLELMNFQEETDYQIDRSAIKAIML 294
            VHKV DEFLE+I+NEHL A+G KKT+D VDVML+L++ Q +T+YQIDRS IKAIML
Sbjct: 241 SVHKVYDEFLEKIINEHLVARGGKKTRDFVDVMLDLIDSQ-QTEYQIDRSTIKAIML 295

BLAST of Chy2G029510 vs. ExPASy TrEMBL
Match: A0A1S3B1M7 (cytochrome P450 CYP736A12-like OS=Cucumis melo OX=3656 GN=LOC103485013 PE=3 SV=1)

HSP 1 Score: 414.8 bits (1065), Expect = 3.1e-112
Identity = 213/297 (71.72%), Postives = 248/297 (83.50%), Query Frame = 0

Query: 1   MALIFAIVSTLALLALTL----LFKFYPFKAQKLPPGPIGFPFVGSLHLLGKLPHRDFHR 60
           MA I  +V    LLAL +    LFK    K +KL PGP GFP  G LHLLGKLPHR+ H+
Sbjct: 1   MASILVVVVITLLLALLISHSWLFK-TQIKPKKLLPGPKGFPIFGCLHLLGKLPHRNLHK 60

Query: 61  LSQKYGPIMHIKLGLVPTIIVSSPKAAELFLKTHDLVFASRPLIEASKQMNYGQKNLVFA 120
           LSQKYGPIM +KLG+VPTIIVSSP+AA+LFLKTHDL+FA+RP ++ASK ++Y QKNLVF+
Sbjct: 61  LSQKYGPIMSMKLGIVPTIIVSSPQAAKLFLKTHDLIFANRPSLQASKHISYQQKNLVFS 120

Query: 121 PYGPYWRNMRKMCTLELLSNLKINSFMPMRKHELGLLIEYLKEAAQNKAVVNLSAKVTSL 180
           PYGPYWRNMRKMCTLELLSN KINSFMP+RKHELGLLIEY KEAA NK VVNLSAKVTSL
Sbjct: 121 PYGPYWRNMRKMCTLELLSNHKINSFMPIRKHELGLLIEYFKEAAHNKTVVNLSAKVTSL 180

Query: 181 TTDLICLMAFGKKYGDEEIDERGFKAVLQEGSQLAATPNLGDFFPFIARFDVQRLNNRMQ 240
           TTD+ICLMAFGKKYGDE+ DERGFKAV+QEG QLAA PNLGDF P IA  D+Q    +++
Sbjct: 181 TTDIICLMAFGKKYGDEDFDERGFKAVIQEGMQLAAAPNLGDFIPTIAWLDLQGFTRKIK 240

Query: 241 CVHKVLDEFLERIVNEHLEAKGDKKTKDLVDVMLELMNFQEETDYQIDRSAIKAIML 294
            VHKV D+FL++I+NEHL A+G KKT+D VDVML+L++ Q +T+YQIDRSAIKAIML
Sbjct: 241 RVHKVYDDFLDKIINEHLVARGRKKTRDFVDVMLDLIDSQ-QTEYQIDRSAIKAIML 295

BLAST of Chy2G029510 vs. NCBI nr
Match: XP_011657998.2 (cytochrome P450 CYP736A12 [Cucumis sativus])

HSP 1 Score: 563 bits (1452), Expect = 1.54e-198
Identity = 285/293 (97.27%), Postives = 288/293 (98.29%), Query Frame = 0

Query: 1   MALIFAIVSTLALLALTLLFKFYPFKAQKLPPGPIGFPFVGSLHLLGKLPHRDFHRLSQK 60
           MALIFAI+STLALLALTLLFKFYPFKAQKLPPGPIGFPFVGSLHLLGKLPHRDFH LSQK
Sbjct: 1   MALIFAIISTLALLALTLLFKFYPFKAQKLPPGPIGFPFVGSLHLLGKLPHRDFHILSQK 60

Query: 61  YGPIMHIKLGLVPTIIVSSPKAAELFLKTHDLVFASRPLIEASKQMNYGQKNLVFAPYGP 120
           YGPIMHIKLGLVPTIIVSSPKAAELFLKTHDLVFASRPL+EASKQMNYGQKNLVFAPYGP
Sbjct: 61  YGPIMHIKLGLVPTIIVSSPKAAELFLKTHDLVFASRPLLEASKQMNYGQKNLVFAPYGP 120

Query: 121 YWRNMRKMCTLELLSNLKINSFMPMRKHELGLLIEYLKEAAQNKAVVNLSAKVTSLTTDL 180
           YWRNMRKMCTLELLSNLKINSFMPMRKHELGLLIEYLKE A NKAVVNLSAKVTSLTTDL
Sbjct: 121 YWRNMRKMCTLELLSNLKINSFMPMRKHELGLLIEYLKEVAHNKAVVNLSAKVTSLTTDL 180

Query: 181 ICLMAFGKKYGDEEIDERGFKAVLQEGSQLAATPNLGDFFPFIARFDVQRLNNRMQCVHK 240
           ICLMAFGKKYGDEEIDERGFKA +QEGSQLAATPNLGDFFPFIARFDVQRLNNRMQCVHK
Sbjct: 181 ICLMAFGKKYGDEEIDERGFKATIQEGSQLAATPNLGDFFPFIARFDVQRLNNRMQCVHK 240

Query: 241 VLDEFLERIVNEHLEAKGDKKTKDLVDVMLELMNFQEETDYQIDRSAIKAIML 293
           VLD FLERIVNEHLEAKGDKKTKDLVDVMLELMNFQEETDYQIDRSAIKAIML
Sbjct: 241 VLDGFLERIVNEHLEAKGDKKTKDLVDVMLELMNFQEETDYQIDRSAIKAIML 293

BLAST of Chy2G029510 vs. NCBI nr
Match: XP_038881390.1 (cytochrome P450 71AU50-like [Benincasa hispida])

HSP 1 Score: 489 bits (1259), Expect = 3.15e-169
Identity = 248/293 (84.64%), Postives = 267/293 (91.13%), Query Frame = 0

Query: 1   MALIFAIVSTLALLALTLLFKFYPFKAQKLPPGPIGFPFVGSLHLLGKLPHRDFHRLSQK 60
           MALIFAI+S L LL+L L FK YPFK QKLPPGP GFP +GSLHLLGKLPHRDF  LSQK
Sbjct: 1   MALIFAIISILFLLSLALPFKLYPFKTQKLPPGPTGFPIIGSLHLLGKLPHRDFQALSQK 60

Query: 61  YGPIMHIKLGLVPTIIVSSPKAAELFLKTHDLVFASRPLIEASKQMNYGQKNLVFAPYGP 120
           YGPIMHIKLGLVPTII+SS  AAELFLKTHDL+FASRPL EASKQMNYGQK+LVFAPYGP
Sbjct: 61  YGPIMHIKLGLVPTIIISSSYAAELFLKTHDLIFASRPLSEASKQMNYGQKDLVFAPYGP 120

Query: 121 YWRNMRKMCTLELLSNLKINSFMPMRKHELGLLIEYLKEAAQNKAVVNLSAKVTSLTTDL 180
           YWRNMRKMCTLELLSNLKINSF  +RKHE+ LLIEYLK+A+ +KAVVNLS KVTSLTTD+
Sbjct: 121 YWRNMRKMCTLELLSNLKINSFKSIRKHEVELLIEYLKKASYSKAVVNLSCKVTSLTTDI 180

Query: 181 ICLMAFGKKYGDEEIDERGFKAVLQEGSQLAATPNLGDFFPFIARFDVQRLNNRMQCVHK 240
            CLM FGKKY D+E DERGFKAV+QEGSQLAATPN GDFFPFIARFD+QRL+NRM+ VHK
Sbjct: 181 TCLMVFGKKYEDQEFDERGFKAVVQEGSQLAATPNWGDFFPFIARFDIQRLSNRMKNVHK 240

Query: 241 VLDEFLERIVNEHLEAKGDKKTKDLVDVMLELMNFQEETDYQIDRSAIKAIML 293
           VLDEFLERI+NEHLEAKG+KKTKD VDVMLELMNFQE TDYQIDRSAIKAIML
Sbjct: 241 VLDEFLERIINEHLEAKGEKKTKDFVDVMLELMNFQE-TDYQIDRSAIKAIML 292

BLAST of Chy2G029510 vs. NCBI nr
Match: XP_031742569.1 (cytochrome P450 CYP736A12-like [Cucumis sativus] >KAE8647573.1 hypothetical protein Csa_004323 [Cucumis sativus])

HSP 1 Score: 430 bits (1106), Expect = 7.76e-146
Identity = 223/297 (75.08%), Postives = 249/297 (83.84%), Query Frame = 0

Query: 1   MALIFAIVSTLALLALTLLFKFYPFKAQ----KLPPGPIGFPFVGSLHLLGKLPHRDFHR 60
           MALI  +V  + LL   L+   + FK+Q    K PPGP GFP  G LHLLGKLPHR+ H 
Sbjct: 1   MALILVVVVLITLLLALLISHSWLFKSQIKPKKSPPGPKGFPIFGCLHLLGKLPHRNLHE 60

Query: 61  LSQKYGPIMHIKLGLVPTIIVSSPKAAELFLKTHDLVFASRPLIEASKQMNYGQKNLVFA 120
           LSQKYGPIM +KLGLVPTIIVSSP AA+LFLKTHDL+FASRP  +ASK ++Y QKNLVFA
Sbjct: 61  LSQKYGPIMSMKLGLVPTIIVSSPHAAQLFLKTHDLIFASRPSSQASKHISYQQKNLVFA 120

Query: 121 PYGPYWRNMRKMCTLELLSNLKINSFMPMRKHELGLLIEYLKEAAQNKAVVNLSAKVTSL 180
           PYGPYWRNMRKMCTLELLSNLKINSFMPMRKHELGLLIEYLKEAA NKAVVNLSAKVTSL
Sbjct: 121 PYGPYWRNMRKMCTLELLSNLKINSFMPMRKHELGLLIEYLKEAAHNKAVVNLSAKVTSL 180

Query: 181 TTDLICLMAFGKKYGDEEIDERGFKAVLQEGSQLAATPNLGDFFPFIARFDVQRLNNRMQ 240
           TTD+ICLMAFGKKYGDEEIDERGFKAV+QEG QL+A PNLGDF P IA  D+Q    +M+
Sbjct: 181 TTDIICLMAFGKKYGDEEIDERGFKAVIQEGMQLSAAPNLGDFIPAIAWLDLQGFTRKMK 240

Query: 241 CVHKVLDEFLERIVNEHLEAKGDKKTKDLVDVMLELMNFQEETDYQIDRSAIKAIML 293
            VHKV DEFLE+I+NEHL A+G KKT+D VDVML+L+  Q+ T+YQIDRSAIKAIML
Sbjct: 241 RVHKVYDEFLEKIINEHLVARGGKKTRDFVDVMLDLIGSQQ-TEYQIDRSAIKAIML 296

BLAST of Chy2G029510 vs. NCBI nr
Match: XP_022132547.1 (cytochrome P450 CYP736A12-like [Momordica charantia])

HSP 1 Score: 429 bits (1102), Expect = 1.92e-145
Identity = 218/281 (77.58%), Postives = 243/281 (86.48%), Query Frame = 0

Query: 13  LLALTLLFKFYPFKAQKLPPGPIGFPFVGSLHLLGKLPHRDFHRLSQKYGPIMHIKLGLV 72
           LLAL LLFK + FK + LPPGP GF   GSLHLLGKLPHRD  +LSQKYGPIMH+KLGLV
Sbjct: 11  LLALALLFKPWLFKPKNLPPGPKGFSIFGSLHLLGKLPHRDLQKLSQKYGPIMHMKLGLV 70

Query: 73  PTIIVSSPKAAELFLKTHDLVFASRPLIEASKQMNYGQKNLVFAPYGPYWRNMRKMCTLE 132
           PTIIVSSP AAELFLKTHDLVFASRPL EASKQMNY QKNLVFAPYG YWRNMRKMCTLE
Sbjct: 71  PTIIVSSPSAAELFLKTHDLVFASRPLSEASKQMNYCQKNLVFAPYGSYWRNMRKMCTLE 130

Query: 133 LLSNLKINSFMPMRKHELGLLIEYLKEAAQNKAVVNLSAKVTSLTTDLICLMAFGKKYGD 192
           LLS+ KINSF  MRK EL LLI++L+EAA N   VNLS+KVTSLTTD+ CLM FG KY D
Sbjct: 131 LLSSRKINSFKSMRKRELALLIDHLREAADNGVAVNLSSKVTSLTTDMTCLMVFGMKYED 190

Query: 193 EEIDERGFKAVLQEGSQLAATPNLGDFFPFIARFDVQRLNNRMQCVHKVLDEFLERIVNE 252
           ++ DERGFKAV+QEGSQLAATPNLGDFFPFIA  D+QRLN +M+ VHK LD+FLERIVN+
Sbjct: 191 KDFDERGFKAVVQEGSQLAATPNLGDFFPFIAGLDLQRLNKQMKSVHKALDDFLERIVND 250

Query: 253 HLEAKGDKKTKDLVDVMLELMNFQEETDYQIDRSAIKAIML 293
           HLE+K ++KTKD VDVML++M+FQE T+YQIDR AIKAIML
Sbjct: 251 HLESKDEEKTKDFVDVMLDVMSFQE-TEYQIDRPAIKAIML 290

BLAST of Chy2G029510 vs. NCBI nr
Match: XP_038883633.1 (cytochrome P450 71AU50-like [Benincasa hispida])

HSP 1 Score: 426 bits (1096), Expect = 1.89e-144
Identity = 221/292 (75.68%), Postives = 248/292 (84.93%), Query Frame = 0

Query: 7   IVSTLALLALTLLF-KFYPFKAQ----KLPPGPIGFPFVGSLHLLGKLPHRDFHRLSQKY 66
           +V TLALL L  L  K + FK Q    KL PGP GFP +GSLHLLGKLPHR+ HRLSQKY
Sbjct: 6   VVLTLALLLLAFLISKSWLFKNQIKSKKLAPGPKGFPILGSLHLLGKLPHRNLHRLSQKY 65

Query: 67  GPIMHIKLGLVPTIIVSSPKAAELFLKTHDLVFASRPLIEASKQMNYGQKNLVFAPYGPY 126
           GPIM +KLGLV TIIVSSP AAELFLKTHDL+FASRP  +ASK ++Y QKNLVF+PYGPY
Sbjct: 66  GPIMSMKLGLVSTIIVSSPHAAELFLKTHDLIFASRPSSQASKHISYQQKNLVFSPYGPY 125

Query: 127 WRNMRKMCTLELLSNLKINSFMPMRKHELGLLIEYLKEAAQNKAVVNLSAKVTSLTTDLI 186
           WRN+RKMCTLELLSNLKINSFMPMRKHELGL+IEYLKEAA NK VVNLS+KVTSLTTD+I
Sbjct: 126 WRNIRKMCTLELLSNLKINSFMPMRKHELGLMIEYLKEAAHNKVVVNLSSKVTSLTTDII 185

Query: 187 CLMAFGKKYGDEEIDERGFKAVLQEGSQLAATPNLGDFFPFIARFDVQRLNNRMQCVHKV 246
           CLMAFGKKY DEE +ERGFKAV+QEG QLAA PNLGDF P IA  D+Q L  +M+CVHKV
Sbjct: 186 CLMAFGKKYEDEEFNERGFKAVIQEGMQLAAAPNLGDFIPAIAWLDLQGLTRQMKCVHKV 245

Query: 247 LDEFLERIVNEHLEAKGDKKTKDLVDVMLELMNFQEETDYQIDRSAIKAIML 293
            DEFLE+I+NEHLE +G KKTKD VDVML+L++ Q+ T+YQIDRSAIKA ML
Sbjct: 246 YDEFLEKIINEHLEVRGGKKTKDFVDVMLDLIDSQQ-TEYQIDRSAIKATML 296

BLAST of Chy2G029510 vs. TAIR 10
Match: AT1G13080.1 (cytochrome P450, family 71, subfamily B, polypeptide 2 )

HSP 1 Score: 196.8 bits (499), Expect = 2.6e-50
Identity = 109/298 (36.58%), Postives = 188/298 (63.09%), Query Frame = 0

Query: 3   LIFAIVSTLALLALTLLFKFYPFKAQKLPPGPIGFPFVGSLHLLGKLPHRDFHRLSQKYG 62
           L F +VS L +++ ++  K        LPP P   P +G+LH L  LPHR FH+LS KYG
Sbjct: 5   LCFFLVSLLTIVS-SIFLKQNKTSKFNLPPSPSSLPIIGNLHHLAGLPHRCFHKLSIKYG 64

Query: 63  PIMHIKLGLVPTIIVSSPKAAELFLKTHDLVFASRPLIEASKQMNYGQKNLVFAPYGPYW 122
           P++ ++LG VP +++SS +AAE  LKT+DL   SRP    S +++YG K++ FAPYG YW
Sbjct: 65  PLVFLRLGSVPVVVISSSEAAEAVLKTNDLECCSRPKTVGSGKLSYGFKDITFAPYGEYW 124

Query: 123 RNMRKMCTLELLSNLKINSFMPMRKHELGLLIEYLKEAAQNKAVVNLSAKVTSLTTDLIC 182
           R +RK+  +EL S+ K+ SF  +R+ E+  +++ + E+A  ++ V+LS    SLT  +IC
Sbjct: 125 REVRKLAVIELFSSKKVQSFRYIREEEVDFVVKKVSESALKQSPVDLSKTFFSLTASIIC 184

Query: 183 LMAFGKKYGDE--EIDERGFKAVLQEGSQLAATPNLGDFFP-FIARFDVQRLNNRMQCVH 242
            +A G+ + +    ID+   + ++ E ++   T    DFFP  + RF V  L  R + ++
Sbjct: 185 RVALGQNFNESGFVIDQDRIEELVTESAEALGTFTFSDFFPGGLGRF-VDWLFQRHKKIN 244

Query: 243 KV---LDEFLERIVNEHLEAKGDKKTKDLVDVMLELMNFQEETD-YQIDRSAIKAIML 294
           KV   LD F + ++++HL+ +G +K +D+V ++L++++ QE++D ++++   +KAI++
Sbjct: 245 KVFKELDAFYQHVIDDHLKPEG-RKNQDIVTLILDMIDKQEDSDSFKLNMDNLKAIVM 299

BLAST of Chy2G029510 vs. TAIR 10
Match: AT3G26200.1 (cytochrome P450, family 71, subfamily B, polypeptide 22 )

HSP 1 Score: 191.0 bits (484), Expect = 1.4e-48
Identity = 103/289 (35.64%), Postives = 172/289 (59.52%), Query Frame = 0

Query: 11  LALLALTLLF--KFYPFKAQKLPPGPIGFPFVGSLHLLGKLPHRDFHRLSQKYGPIMHIK 70
           L LL L L+F  K  P K  KLPPGP+G P +G+LH LGK  HR FH+LSQ YGP+M + 
Sbjct: 8   LLLLPLFLIFFKKLSPSKG-KLPPGPLGLPIIGNLHQLGKSLHRSFHKLSQNYGPVMFLH 67

Query: 71  LGLVPTIIVSSPKAAELFLKTHDLVFASRPLIEASKQMNYGQKNLVFAPYGPYWRNMRKM 130
            G+VP ++VS+ +AAE  LKTHDL   +RP + A+K  +Y  K++ FA YG  WR MRK+
Sbjct: 68  FGVVPVVVVSTREAAEEVLKTHDLETCTRPKLTATKLFSYNYKDIGFAQYGDDWREMRKL 127

Query: 131 CTLELLSNLKINSFMPMRKHELGLLIEYLKEAAQNKAVVNLSAKVTSLTTDLICLMAFGK 190
             LEL S+ K+ +F  +R+ E  +L+  L ++A+ + +V+L   + S T  ++C +AFG+
Sbjct: 128 AMLELFSSKKLKAFRYIREEESEVLVNKLSKSAETRTMVDLRKALFSYTASIVCRLAFGQ 187

Query: 191 KYGD-EEIDERGFKAVLQEGSQLAATPNLGDFFPFIARFDVQRLNNRMQCVHKV---LDE 250
            + + + +D    + ++ E      +    DFFP    + + R++ +   +HK    L  
Sbjct: 188 NFHECDFVDMDKVEDLVLESETNLGSFAFTDFFPAGLGWVIDRISGQHSELHKAFARLSN 247

Query: 251 FLERIVNEHLEAKGDKKTKDLVDVMLELMNFQEET-DYQIDRSAIKAIM 293
           F + ++++HL+    +   D++ VML+++N + +   +Q+    +K +M
Sbjct: 248 FFQHVIDDHLKPGQSQDHSDIIGVMLDMINKESKVGSFQVTYDHLKGVM 295

BLAST of Chy2G029510 vs. TAIR 10
Match: AT3G48270.1 (cytochrome P450, family 71, subfamily A, polypeptide 26 )

HSP 1 Score: 188.3 bits (477), Expect = 9.2e-48
Identity = 104/294 (35.37%), Postives = 177/294 (60.20%), Query Frame = 0

Query: 1   MALIFAIVSTLALLALTLLFKFYPFKAQKLPPGPIGFPFVGSLHLLGKLPHRDFHRLSQK 60
           M + F + S + ++ + +  K    K +   P P G P +G+LH LG+ PHR    LS +
Sbjct: 2   MIMFFLLCSIIFVVTIIIFRKQKRGKKRNTLPSPPGLPLIGNLHQLGRHPHRSLCSLSHR 61

Query: 61  YGPIMHIKLGLVPTIIVSSPKAAELFLKTHDLVFASRPLIEASKQMNYGQKNLVFAPYGP 120
           YGP+M +  G VP ++VSS + A   LKTHD VFASRP  +  +++ Y + ++  APYG 
Sbjct: 62  YGPLMLLHFGRVPVLVVSSAELARDVLKTHDRVFASRPRSKIFEKLLYDKHDVASAPYGE 121

Query: 121 YWRNMRKMCTLELLSNLKINSFMPMRKHELGLLIEYLKEAAQNKAVVNLSAKVTSLTTDL 180
           YWR M+ +C L L SN  + SF  +R+ E+ L++E ++++      VNLS  + SLT D+
Sbjct: 122 YWRQMKSVCVLHLFSNKMVRSFREVREEEISLMMEKIRKSI--SLPVNLSKILVSLTNDV 181

Query: 181 ICLMAFGKKYGDEEIDERGFKAVLQEGSQLAATPNLGDFFPFIARFD-VQRLNNRMQCVH 240
           IC +A G+KYG     E  FK +++  ++L  T ++G + P++A  D ++ L+ +++   
Sbjct: 182 ICKVALGRKYG----GETDFKELMERLNKLLGTFSVGSYVPWLAWIDWIRGLDCQLEKTA 241

Query: 241 KVLDEFLERIVNEHLEAKGDKKTKDLVDVMLELMNFQEETDYQIDRSAIKAIML 294
             +D+F ER+V +H++  G++   D VDV+L +    +   ++I+R +IKAI++
Sbjct: 242 NDVDKFFERVVQDHVD--GNRDMTDFVDVLLAIQR-DKTVGFEINRVSIKAIVM 286

BLAST of Chy2G029510 vs. TAIR 10
Match: AT3G48290.2 (cytochrome P450, family 71, subfamily A, polypeptide 24 )

HSP 1 Score: 187.2 bits (474), Expect = 2.0e-47
Identity = 106/300 (35.33%), Postives = 177/300 (59.00%), Query Frame = 0

Query: 1   MALIFAIVSTLALLALTLLFKFYPFKAQKLPPGPIGFPFVGSLHLLGKLPHRDFHRLSQK 60
           M +I  ++ ++ L+ +    K    K    PP P   P + +LH LG+ PHR    LS +
Sbjct: 2   MMMIILLLCSIILITILFFKKQSRGKKSNAPPSPPRLPLIRNLHQLGRHPHRSLCSLSHR 61

Query: 61  YGPIMHIKLGLVPTIIVSSPKAAELFLKTHDLVFASRPLIEASKQMNYGQKNLVFAPYGP 120
           YGP+M +  G VP ++VSS  AA+  LKTHD VFASRP  +   ++ Y  +++  APYG 
Sbjct: 62  YGPLMLLHFGSVPVLVVSSADAAKDVLKTHDRVFASRPRSKIFDKIFYNGRDVALAPYGE 121

Query: 121 YWRNMRKMCTLELLSNLKINSFMPMRKHELGLLIEYLKEAAQNKAVVNLSAKVTSLTTDL 180
           YWR M+ +C L L SN  + SF  +R+ E+ L+IE ++ ++  +  +NLS  + +LT ++
Sbjct: 122 YWRQMKSVCVLHLFSNKMVRSFRDVRQEEISLMIEKIRISSSLR--INLSEILVNLTNNV 181

Query: 181 ICLMAFGKKYGDEEIDERGFKAVLQEGSQLAATPNLGDFFPFIARFD-VQRLNNRMQCVH 240
           IC +A G+KYG     +  FK +++  ++L    ++G +  ++A  D ++ L+ ++  + 
Sbjct: 182 ICRVALGRKYG----GKTDFKDLMKRLTRLLGEFSVGSYVSWLAWIDWIRGLDGQLIKIS 241

Query: 241 KVLDEFLERIVNEHLEAKGDKKTKDLVDVMLELMNFQEETDYQIDRSAIKAIMLLDKRRE 300
             LDEFLER+V +H++  G K   D VD +L +   ++   ++IDR +IKAI+L+  R E
Sbjct: 242 NDLDEFLERVVQDHVDGDGHK--NDFVDFLLTIER-EKSVGFEIDRLSIKAIILVKGRYE 292

BLAST of Chy2G029510 vs. TAIR 10
Match: AT3G48310.1 (cytochrome P450, family 71, subfamily A, polypeptide 22 )

HSP 1 Score: 186.4 bits (472), Expect = 3.5e-47
Identity = 108/296 (36.49%), Postives = 178/296 (60.14%), Query Frame = 0

Query: 1   MALIFAIVSTLALLALTLLFKFYPFKAQK--LPPGPIGFPFVGSLHLLGKLPHRDFHRLS 60
           M  +  I+    ++ +T+LF     K +K   P  P   P +G+LH LG+ PHR    LS
Sbjct: 1   MESMIRIILLSLIIFITILFFIKQKKGKKSNTPASPPRLPLIGNLHQLGRHPHRSLCSLS 60

Query: 61  QKYGPIMHIKLGLVPTIIVSSPKAAELFLKTHDLVFASRPLIEASKQMNYGQKNLVFAPY 120
            +YGP+M ++ GLVP ++VSS   A   LKT+D VFASRP  +  +++ Y  +++  APY
Sbjct: 61  NRYGPLMLLRFGLVPVLVVSSADVARDILKTYDRVFASRPRSKIFEKIFYEARDVALAPY 120

Query: 121 GPYWRNMRKMCTLELLSNLKINSFMPMRKHELGLLIEYLKEAAQNKAVVNLSAKVTSLTT 180
           G YWR M+ +C L LL+N  + SF  +R+ E+ L++E +++++  +  VNLS  + SLT 
Sbjct: 121 GEYWRQMKSVCVLHLLTNKMVRSFRNVRQEEISLMMEKIQKSSSLQ--VNLSELLGSLTN 180

Query: 181 DLICLMAFGKKYGDEEIDERGFKAVLQEGSQLAATPNLGDFFPFIARFD-VQRLNNRMQC 240
           D+I  +A G+KY     DE  FK +++  ++L     +G + P++A  D +  L+ +++ 
Sbjct: 181 DVISRVALGRKYS----DETDFKELMKRLTKLLGEFCVGTYVPWLAWIDWISGLDGQLKK 240

Query: 241 VHKVLDEFLERIVNEHLEAKGDKKTKDLVDVMLELMNFQEETDYQIDRSAIKAIML 294
               LDEFLE++V +H +  GD +  D VDV+L +   ++   ++IDR +IKAI+L
Sbjct: 241 TGNDLDEFLEKVVQDHED--GDAQRTDFVDVLLRIQR-EKSVGFEIDRLSIKAIIL 287

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A0A068Q5V62.6e-8756.40Cytochrome P450 71AU50 OS=Prunus mume OX=102107 GN=CYP71AU50 PE=2 SV=1[more]
H2DH181.2e-6044.11Cytochrome P450 CYP736A12 OS=Panax ginseng OX=4054 PE=2 SV=1[more]
P244659.5e-5038.13Cytochrome P450 71A1 OS=Persea americana OX=3435 GN=CYP71A1 PE=1 SV=2[more]
O657883.6e-4936.58Cytochrome P450 71B2 OS=Arabidopsis thaliana OX=3702 GN=CYP71B2 PE=2 SV=2[more]
A0A068Q6L26.2e-4937.71Cytochrome P450 736A117 OS=Prunus mume OX=102107 GN=CYP736A117 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KJ011.5e-15993.27Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G501340 PE=3 SV=1[more]
A0A6J1BU497.2e-11776.11cytochrome P450 CYP736A12-like OS=Momordica charantia OX=3673 GN=LOC111005380 PE... [more]
A0A5A7SY995.7e-11473.40Cytochrome P450 CYP736A12-like protein OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A1S3B1755.7e-11473.40cytochrome P450 CYP736A12-like OS=Cucumis melo OX=3656 GN=LOC103485010 PE=3 SV=1[more]
A0A1S3B1M73.1e-11271.72cytochrome P450 CYP736A12-like OS=Cucumis melo OX=3656 GN=LOC103485013 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_011657998.21.54e-19897.27cytochrome P450 CYP736A12 [Cucumis sativus][more]
XP_038881390.13.15e-16984.64cytochrome P450 71AU50-like [Benincasa hispida][more]
XP_031742569.17.76e-14675.08cytochrome P450 CYP736A12-like [Cucumis sativus] >KAE8647573.1 hypothetical prot... [more]
XP_022132547.11.92e-14577.58cytochrome P450 CYP736A12-like [Momordica charantia][more]
XP_038883633.11.89e-14475.68cytochrome P450 71AU50-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT1G13080.12.6e-5036.58cytochrome P450, family 71, subfamily B, polypeptide 2 [more]
AT3G26200.11.4e-4835.64cytochrome P450, family 71, subfamily B, polypeptide 22 [more]
AT3G48270.19.2e-4835.37cytochrome P450, family 71, subfamily A, polypeptide 26 [more]
AT3G48290.22.0e-4735.33cytochrome P450, family 71, subfamily A, polypeptide 24 [more]
AT3G48310.13.5e-4736.49cytochrome P450, family 71, subfamily A, polypeptide 22 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 58..77
score: 31.92
coord: 176..194
score: 22.29
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 24..294
e-value: 6.8E-56
score: 191.7
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 31..291
IPR001128Cytochrome P450PFAMPF00067p450coord: 31..290
e-value: 2.8E-37
score: 128.5
NoneNo IPR availablePANTHERPTHR47943CYTOCHROME P450 93A3-LIKEcoord: 9..293
NoneNo IPR availablePANTHERPTHR47943:SF2CYTOCHROME P450 FAMILY 71 PROTEINcoord: 9..293

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy2G029510.1Chy2G029510.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen