Chy2G028100 (gene) Cucumber (hystrix) v1

Overview
NameChy2G028100
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionAspartyl protease family protein 1-like
LocationchrH02: 3269844 .. 3273999 (+)
RNA-Seq ExpressionChy2G028100
SyntenyChy2G028100
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCACCGATGATTCACAACTCAAACCTGTTGATGCGAAGGTTACTTTAGGGTGAGTTTTGTTGAAGCTGTCTTAGTTATATCCGCTTGTGCTGAAATTTCTTCGAGGGTCATTTCGTTTAGTTTTTAATAGATTTTGTTTTCTGTCAAAATAATAACAAAGTAGTCAAATACAAAATCAATGGTGTTTTAAGAAACTATGTACTCGACTGGTTATACTTGATTCTGTAAGACTGTAACTTGACCAATGCTACAAGAAAAGTCATGATAGTCGTCTTCGAGGGAGCTCAATGAAAGTCTTAGCACTAAGTTTATTGTATCACAACAATACCATCTGTTATAAATCTTGTGCAGTATGTTGAGTTTTTGCATTATTTAAATGGGGTTCTTCATGGCAGATGTGGTAAGGTCCAGACTGGTAAATTCGCAAATGTCACAGCTGGCAATGGTCTTATTGGGCTTGGCATGGGAAAGCTATCGGTTCCAAGCTTCTTAGCAAGCCAAGGTCTGACCGCAGATTCATTCTCTATGTGTTTTGGATATTATGGTTATGGGAGAATCGATTTTGGAGACATAGGCCCAGTAGGACAGAGAGAAACGCCCTTCAATCCTGCATCGTAAGAACTTCTCACTGTTTACACGTGCAAAAGTAATAATCTACAACCTTCATTTCTCTTGGAGAAGCTGGATTTTGTAACTAGATTGTCTTGTTTGTTGGCAGTTTATCCTACAATGTCACCGTCCTTCAGATAATTGTGACAAATAGACCCACAAATGTTCACTTCACTGCAATTATCGACACTGGTTCCTCTTTTACATACCTAACTGATCCATTTTACTCTATTATTTTTGAGAAAGTAAGCTTTTTGCAATTTCACAGTTCTCTTTGGAAGTGCCACATGAACCCATGAAATCTCTAGTTTATATAGAGAATTATTGTGCAGATAGATGCAGATACGGAATTAGAGCTCATTACATCTGATTCTGATTTCCCATTTGAGTACTGCTACCGAATTTCGTAAGTAGCTGTAACAAACTATTTTTTTTGTCTTTCAAAAGATCTATAGATTCTGATTCCCATATTCGATTTTGATCAGTCTGGGAACAATCTTTTTAACACCACCTGTGAATTTTACAATGGAGGGTGGACGTAAGTTTGACGTCATTACTTCGCCTGTCATCGTTCAAACTGATGCGAGTGTAATTTCATTTTTTAGATGAAATAACATGAGAGACAAAGAGAATGTTATATGTTTGCTTAGTTGGCTTTCTTTTTGGTTTTTTCTTGATTTGGGTTTCTGTTGGCTTTAAGATGGACCTGCCCTTTGTCTAGCCATTGTCAAAAGCACTGATATTAACGTAATTGGAAGTGAGTGCCACTGTATCTCCATCTGCCATTTTATCATCATGTGTATTCGATTAGTTCTAATTTTAAAAGGTTTTAAATTGCTTTTGTCATGTTCATTTTTGTACACCTATAACTTATAACTACAATCGCATATCATCCAATTTGGTTTTGAACGGTTAAAAGGTATGTTCAAGAGTAATTTTAACTATTTTAATATCATTTTTGAACATACCTCTTTCAGTAGTGTTGATGTTGGAAAAAGATTAGGAACTCTCTCAAGTATTCTCTTTCTTGTAGGCAACTTATTGGGTGGTTATCGCATTGTCTTCAATCGTGAAAAGATGACGTTGGGATGGAAAGAAGTAGAAGATTGTGAGTATTTCTTTACTTGTAAAACTCCTTTTTGTTTTTCTGAATTTTTCCTAATGGTCGTTTTTTTTAATATAGGTGACAGTTATGTTCCCAACACTCCCTCCGACGACTCCCCTCCACAGTCCAGAGACTCCCCTCCAGCAAATTCTACTCCAAGAAAAAGCAACAGCACGCAACCATCACCGAAAATTGTTGCGGGTGATGCCATGCTATTAAATCCAATTGTCTCTCTATGTAATGGAAAAGTATTTTTTCTTTCTCAGTATTCTATGAAAAAGGTGGGGCTAATTCTAGAAGTTGATGTTGGTTGTTCTCAGTTAGTACATTCTTTTTTTGTTTCTTCTACAGTTTTGGTCGAATCATTACAGTTCAAATGCTTCATCAACCAGCATTCATGTGCACTGCAGCAGTTCTTTGTGCGAGCTTGCAAACCAGTGTTCTTCGAACAAAAGTTCTTGTCCTTACCAAACTCATTACCTGTCTCAAAATTCTTCATCTACTGGGTACTTGGTACAGGACATATTGCACATGGCCACCGATGATTCACAACTCAAACCTGTTGATGTGAAGGTTACTTTAGGGTGAGTTTTATTGAATCTGTCTTAGTTATATTTCTTGTGCTGAAATTTCTTTGAGGGTAATTTCGTTTAGTGCTTAATAGATTTAGTTTTCTGTTAAGATAACAAAGTAATGAAATACAAAATCAATGGTATTTTAAGAAACTATGTACTCAACTGGTTATACTTGATTCTGGAAGACTGTAACTTGATCAATGCTACAAGAAAAGTCTTGGTATTCATCTTCGAGAGAGCTCAATGAAAGTCTTAGCACGTTGTAAGTTTATTGTATCTCAACAATACCATATTATAGAAATCATGTGCAGTCTGCTGAGTTTTTGAATTGTTTAAATGGGGTTCTTCATGGCAGATGTGGTAAGGTCCAGGCTGGTAAATTCTCAAATGTCACAGCTCCCAATGGTCTTATTGGGCTTGGCATGGGAAAGCTATCGATTCCAAGCTTCTTAGCAAGCCAAGGATTAACCCCATATTCATTCTCTATGTGTTTTGGATATTATGGTTATGGGAGAACCGATTTTGGAGACATAGGCCCAGTAGGCCAGAGACAGACGCCCTTCAATCCTGCAAGGTAAGAACTTCTCACTGTTTACATGCGCAAAAGTAATAATCTACGACCTTCATTTCTCTTGGAGAAGCTGGTTTTTGTAACTAGATTGTCTTGTTTGTTGACAGTTTATCCTACAATGTCACCATCCTTCAGATAATTGTGACAAATAGACCCAGAAATGTTCACTTCACTGCAATTATCGACTCTGGCGCCTCTTTTACATACCTGACTGATCCATTTTATTCTATTATTACTGAGAATGTAAGGTTTTTGCAGTTTCGGTTCTCTTAGGAAGTGCCACATGACTCCATGAAATCTCTAGTTTATATAAAGAATTGTTGTGCATCGGCTGCAGCTATGGAGTTGGAGCGCATCAAATCTGATTCATTTGAGTACTGCTACCGACTTTCGTAAGTAGCTGTAACTTGTGTAACAATTTTTTTTTTTTTGTCTTTCAAAAGATCTTCAGATTCCAATTCCCATATTTTAATCCAGTCTGGCAACAATCTTTCAACAACCAAATTTGAATTTTACAATGGAGGGTGGACGTAGGTTTGACTTCATTACTTCGTATGTCAGCGTTGATACTGATGTGAGTGTAATTTCATTTTTTAGATTAAAATGAAATAACATGAGAGTCAGAGAAGATATATGTATGCGTTATTGGCTTTCTTTTTTGTTTTTTCTTGATTTGGGTTTCTGTTGGCTTCAGGATGGACCTGCCCTTTGTCTAGTCATTGTCAAAAGCACTGATATTAATGTAATTGGACGTGAGTGCTCCTGTCTCTTCATCTAATTTATGAAGCCATTTTATCTCCTTGTTTATTCCATTAATTCTCCTGTTTGCAAGTGATTTTAAAAAGTCTAAAGTATGTGCAAGAGTGATTGCGACTATTTTAGAATCACTTTTGAACACCTCTTTAGTAGTGTTGATATTGGAAAAAGATTAGGAACTCTTTCAAGTATTCTCGTTTTCTTGTAGACAACTTCTTGGGTGGGTATCGCATTGTCATCAATCGTGAAAAGATGACGTTGGGATGGAAAGAAGTAGATTGTGAGTATTTCTTTACTTGTAAAACTCCGTTTTGTTTTTCTGAATTTTTTCAGTGTCAGTGATGGTCGTTTTCTTTGATCTAGGTGACAGTTATGACGCCAACACTTCCTCCAACGACTCCCCTCCACCGTTCCGAGACTCTTCTCCGACAACTTCTACTCCAAGAAAAAGCAACAGCACGCAACCATCACCGGAAATTGGGACAGGTGATGCGATGCGATTAAATCCAATTCTCTCTGTGTTGTCATCCTTGTAA

mRNA sequence

ATGGCCACCGATGATTCACAACTCAAACCTGTTGATGCGAAGGTTACTTTAGGATGTGGTAAGGTCCAGACTGGTAAATTCGCAAATGTCACAGCTGGCAATGGTCTTATTGGGCTTGGCATGGGAAAGCTATCGGTTCCAAGCTTCTTAGCAAGCCAAGGTCTGACCGCAGATTCATTCTCTATGTGTTTTGGATATTATGGTTATGGGAGAATCGATTTTGGAGACATAGGCCCAGTAGGACAGAGAGAAACGCCCTTCAATCCTGCATCTTTATCCTACAATGTCACCGTCCTTCAGATAATTGTGACAAATAGACCCACAAATGTTCACTTCACTGCAATTATCGACACTGGTTCCTCTTTTACATACCTAACTGATCCATTTTACTCTATTATTTTTGAGAAAATAGATGCAGATACGGAATTAGAGCTCATTACATCTGATTCTGATTTCCCATTTGAGTACTGCTACCGAATTTCTCTGGGAACAATCTTTTTAACACCACCTGTGAATTTTACAATGGAGGGTGGACATGGACCTGCCCTTTGTCTAGCCATTGTCAAAAGCACTGATATTAACGTAATTGGAAGCAACTTATTGGGTGGTTATCGCATTGTCTTCAATCGTGAAAAGATGACGTTGGGATGGAAAGAAGTAGAAGATTGTGACAGTTATGTTCCCAACACTCCCTCCGACGACTCCCCTCCACAGTCCAGAGACTCCCCTCCAGCAAATTCTACTCCAAGAAAAAGCAACAGCACGCAACCATCACCGAAAATTGTTGCGGGTGATGCCATGCTATTAAATCCAATTGTCTCTCTATGTAATGGAAAATTTTGGTCGAATCATTACAGTTCAAATGCTTCATCAACCAGCATTCATGTGCACTGCAGCAGTTCTTTGTGCGAGCTTGCAAACCAGTGTTCTTCGAACAAAAGTTCTTGTCCTTACCAAACTCATTACCTGTCTCAAAATTCTTCATCTACTGGGTACTTGGTACAGGACATATTGCACATGGCCACCGATGATTCACAACTCAAACCTGTTGATGTGAAGGTTACTTTAGGATGTGGTAAGGTCCAGGCTGGTAAATTCTCAAATGTCACAGCTCCCAATGGTCTTATTGGGCTTGGCATGGGAAAGCTATCGATTCCAAGCTTCTTAGCAAGCCAAGGATTAACCCCATATTCATTCTCTATGTGTTTTGGATATTATGGTTATGGGAGAACCGATTTTGGAGACATAGGCCCAGTAGGCCAGAGACAGACGCCCTTCAATCCTGCAAGTTTATCCTACAATGTCACCATCCTTCAGATAATTGTGACAAATAGACCCAGAAATGTTCACTTCACTGCAATTATCGACTCTGGCGCCTCTTTTACATACCTGACTGATCCATTTTATTCTATTATTACTGAGAATGTAAGTCTGGCAACAATCTTTCAACAACCAAATTTGAATTTTACAATGGAGGGTGGACGTAGGTTTGACTTCATTACTTCGTATGTCAGCGTTGATACTGATGATGGACCTGCCCTTTGTCTAGTCATTGTCAAAAGCACTGATATTAATGTAATTGGACACAACTTCTTGGGTGGGTATCGCATTGTCATCAATCGTGAAAAGATGACGTTGGGATGGAAAGAAGTAGATTGTGACAGTTATGACGCCAACACTTCCTCCAACGACTCCCCTCCACCGTTCCGAGACTCTTCTCCGACAACTTCTACTCCAAGAAAAAGCAACAGCACGCAACCATCACCGGAAATTGGGACAGGTGATGCGATGCGATTAAATCCAATTCTCTCTGTGTTGTCATCCTTGTAA

Coding sequence (CDS)

ATGGCCACCGATGATTCACAACTCAAACCTGTTGATGCGAAGGTTACTTTAGGATGTGGTAAGGTCCAGACTGGTAAATTCGCAAATGTCACAGCTGGCAATGGTCTTATTGGGCTTGGCATGGGAAAGCTATCGGTTCCAAGCTTCTTAGCAAGCCAAGGTCTGACCGCAGATTCATTCTCTATGTGTTTTGGATATTATGGTTATGGGAGAATCGATTTTGGAGACATAGGCCCAGTAGGACAGAGAGAAACGCCCTTCAATCCTGCATCTTTATCCTACAATGTCACCGTCCTTCAGATAATTGTGACAAATAGACCCACAAATGTTCACTTCACTGCAATTATCGACACTGGTTCCTCTTTTACATACCTAACTGATCCATTTTACTCTATTATTTTTGAGAAAATAGATGCAGATACGGAATTAGAGCTCATTACATCTGATTCTGATTTCCCATTTGAGTACTGCTACCGAATTTCTCTGGGAACAATCTTTTTAACACCACCTGTGAATTTTACAATGGAGGGTGGACATGGACCTGCCCTTTGTCTAGCCATTGTCAAAAGCACTGATATTAACGTAATTGGAAGCAACTTATTGGGTGGTTATCGCATTGTCTTCAATCGTGAAAAGATGACGTTGGGATGGAAAGAAGTAGAAGATTGTGACAGTTATGTTCCCAACACTCCCTCCGACGACTCCCCTCCACAGTCCAGAGACTCCCCTCCAGCAAATTCTACTCCAAGAAAAAGCAACAGCACGCAACCATCACCGAAAATTGTTGCGGGTGATGCCATGCTATTAAATCCAATTGTCTCTCTATGTAATGGAAAATTTTGGTCGAATCATTACAGTTCAAATGCTTCATCAACCAGCATTCATGTGCACTGCAGCAGTTCTTTGTGCGAGCTTGCAAACCAGTGTTCTTCGAACAAAAGTTCTTGTCCTTACCAAACTCATTACCTGTCTCAAAATTCTTCATCTACTGGGTACTTGGTACAGGACATATTGCACATGGCCACCGATGATTCACAACTCAAACCTGTTGATGTGAAGGTTACTTTAGGATGTGGTAAGGTCCAGGCTGGTAAATTCTCAAATGTCACAGCTCCCAATGGTCTTATTGGGCTTGGCATGGGAAAGCTATCGATTCCAAGCTTCTTAGCAAGCCAAGGATTAACCCCATATTCATTCTCTATGTGTTTTGGATATTATGGTTATGGGAGAACCGATTTTGGAGACATAGGCCCAGTAGGCCAGAGACAGACGCCCTTCAATCCTGCAAGTTTATCCTACAATGTCACCATCCTTCAGATAATTGTGACAAATAGACCCAGAAATGTTCACTTCACTGCAATTATCGACTCTGGCGCCTCTTTTACATACCTGACTGATCCATTTTATTCTATTATTACTGAGAATGTAAGTCTGGCAACAATCTTTCAACAACCAAATTTGAATTTTACAATGGAGGGTGGACGTAGGTTTGACTTCATTACTTCGTATGTCAGCGTTGATACTGATGATGGACCTGCCCTTTGTCTAGTCATTGTCAAAAGCACTGATATTAATGTAATTGGACACAACTTCTTGGGTGGGTATCGCATTGTCATCAATCGTGAAAAGATGACGTTGGGATGGAAAGAAGTAGATTGTGACAGTTATGACGCCAACACTTCCTCCAACGACTCCCCTCCACCGTTCCGAGACTCTTCTCCGACAACTTCTACTCCAAGAAAAAGCAACAGCACGCAACCATCACCGGAAATTGGGACAGGTGATGCGATGCGATTAAATCCAATTCTCTCTGTGTTGTCATCCTTGTAA

Protein sequence

MATDDSQLKPVDAKVTLGCGKVQTGKFANVTAGNGLIGLGMGKLSVPSFLASQGLTADSFSMCFGYYGYGRIDFGDIGPVGQRETPFNPASLSYNVTVLQIIVTNRPTNVHFTAIIDTGSSFTYLTDPFYSIIFEKIDADTELELITSDSDFPFEYCYRISLGTIFLTPPVNFTMEGGHGPALCLAIVKSTDINVIGSNLLGGYRIVFNREKMTLGWKEVEDCDSYVPNTPSDDSPPQSRDSPPANSTPRKSNSTQPSPKIVAGDAMLLNPIVSLCNGKFWSNHYSSNASSTSIHVHCSSSLCELANQCSSNKSSCPYQTHYLSQNSSSTGYLVQDILHMATDDSQLKPVDVKVTLGCGKVQAGKFSNVTAPNGLIGLGMGKLSIPSFLASQGLTPYSFSMCFGYYGYGRTDFGDIGPVGQRQTPFNPASLSYNVTILQIIVTNRPRNVHFTAIIDSGASFTYLTDPFYSIITENVSLATIFQQPNLNFTMEGGRRFDFITSYVSVDTDDGPALCLVIVKSTDINVIGHNFLGGYRIVINREKMTLGWKEVDCDSYDANTSSNDSPPPFRDSSPTTSTPRKSNSTQPSPEIGTGDAMRLNPILSVLSSL*
Homology
BLAST of Chy2G028100 vs. ExPASy Swiss-Prot
Match: Q8VYV9 (Aspartyl protease family protein 1 OS=Arabidopsis thaliana OX=3702 GN=APF1 PE=2 SV=1)

HSP 1 Score: 250.0 bits (637), Expect = 7.0e-65
Identity = 145/345 (42.03%), Postives = 194/345 (56.23%), Query Frame = 0

Query: 283 NHYSSNASSTSIHVHCSSSLCELANQCSSNKSSCPYQTHYLSQNSSSTGYLVQDILHMAT 342
           N YS NASSTS  V C+S+LC   ++C+S +S CPYQ  YLS  +SSTG LV+D+LH+ +
Sbjct: 152 NIYSPNASSTSTKVPCNSTLCTRGDRCASPESDCPYQIRYLSNGTSSTGVLVEDVLHLVS 211

Query: 343 DDSQLKPVDVKVTLGCGKVQAGKFSNVTAPNGLIGLGMGKLSIPSFLASQGLTPYSFSMC 402
           +D   K +  +VT GCG+VQ G F +  APNGL GLG+  +S+PS LA +G+   SFSMC
Sbjct: 212 NDKSSKAIPARVTFGCGQVQTGVFHDGAAPNGLFGLGLEDISVPSVLAKEGIAANSFSMC 271

Query: 403 FGYYGYGRTDFGDIGPVGQRQTPFN--PASLSYNVTILQIIVTNRPRNVHFTAIIDSGAS 462
           FG  G GR  FGD G V QR+TP N      +YN+T+ +I V     ++ F A+ DSG S
Sbjct: 272 FGNDGAGRISFGDKGSVDQRETPLNIRQPHPTYNITVTKISVGGNTGDLEFDAVFDSGTS 331

Query: 463 FTYLTDPFYSIITENVSLATI-------------------------FQQPNLNFTMEGGR 522
           FTYLTD  Y++I+E+ +   +                         FQ P +N TM+GG 
Sbjct: 332 FTYLTDAAYTLISESFNSLALDKRYQTTDSELPFEYCYALSPNKDSFQYPAVNLTMKGGS 391

Query: 523 RFDFITSYVSVDTDDGPALCLVIVKSTDINVIGHNFLGGYRIVINREKMTLGWKEVDCDS 582
            +      V +   D    CL I+K  DI++IG NF+ GYR+V +REK+ LGWKE DC  
Sbjct: 392 SYPVYHPLVVIPMKDTDVYCLAIMKIEDISIIGQNFMTGYRVVFDREKLILGWKESDC-- 451

Query: 583 YDANTSSNDSPPPFRDSS---PTTS-TPRKSNSTQPSPEIGTGDA 597
           Y   TS+   P     SS   P +S  P  +N     P   T  A
Sbjct: 452 YTGETSARTLPSNRSSSSARPPASSFDPEATNIPSQRPNTSTTSA 494

BLAST of Chy2G028100 vs. ExPASy Swiss-Prot
Match: Q9LX20 (Aspartic proteinase-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=At5g10080 PE=2 SV=1)

HSP 1 Score: 159.8 bits (403), Expect = 9.5e-38
Identity = 114/324 (35.19%), Postives = 154/324 (47.53%), Query Frame = 0

Query: 283 NHYSSNASSTSIHVHCSSSLCELANQCSSNKSSCPYQTHYLSQNSSSTGYLVQDILHMAT 342
           N Y+ ++SSTS    CS  LC+ A+ C S K  CPY  +YLS N+SS+G LV+DILH+  
Sbjct: 150 NEYNPSSSSTSKVFLCSHKLCDSASDCESPKEQCPYTVNYLSGNTSSSGLLVEDILHLTY 209

Query: 343 DDSQ-----LKPVDVKVTLGCGKVQAGKFSNVTAPNGLIGLGMGKLSIPSFLASQGLTPY 402
           + +         V  +V +GCGK Q+G + +  AP+GL+GLG  ++S+PSFL+  GL   
Sbjct: 210 NTNNRLMNGSSSVKARVVIGCGKKQSGDYLDGVAPDGLMGLGPAEISVPSFLSKAGLMRN 269

Query: 403 SFSMCFGYYGYGRTDFGDIGPVGQRQTPF----NPASLSYNVTILQIIVTNR-PRNVHFT 462
           SFS+CF     GR  FGD+GP  Q+ TPF    N     Y V +    + N   +   FT
Sbjct: 270 SFSLCFDEEDSGRIYFGDMGPSIQQSTPFLQLDNNKYSGYIVGVEACCIGNSCLKQTSFT 329

Query: 463 AIIDSGASFTYLTDPFYSIIT--------------ENVSLATIFQQ------PNLNFTME 522
             IDSG SFTYL +  Y  +               E VS    ++       P +     
Sbjct: 330 TFIDSGQSFTYLPEEIYRKVALEIDRHINATSKNFEGVSWEYCYESSAEPKVPAIKLKFS 389

Query: 523 GGRRFDFITSYVSVDTDDG-PALCLVIVKS--TDINVIGHNFLGGYRIVINREKMTLGWK 569
               F             G    CL I  S    I  IG N++ GYR+V +RE M LGW 
Sbjct: 390 HNNTFVIHKPLFVFQQSQGLVQFCLPISPSGQEGIGSIGQNYMRGYRMVFDRENMKLGWS 449

BLAST of Chy2G028100 vs. ExPASy Swiss-Prot
Match: Q9LEW3 (Aspartyl protease AED1 OS=Arabidopsis thaliana OX=3702 GN=AED1 PE=2 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 1.5e-11
Identity = 81/271 (29.89%), Postives = 124/271 (45.76%), Query Frame = 0

Query: 276 CNGKFWSN---HYSSNASSTSIHVHCSSSLCELANQCSSNKSSCPYQTHYLSQNSSSTGY 335
           C G  +S     ++ ++SST  +V CSS +CE A  CS+  S+C Y   Y    S + G+
Sbjct: 163 CLGSCYSQKEPKFNPSSSSTYQNVSCSSPMCEDAESCSA--SNCVYSIVY-GDKSFTQGF 222

Query: 336 LVQDILHMATDDSQLKPVDVKVTLGCGKVQAGKFSNVTAPNGLIGLGMGKLSIPSFLASQ 395
           L ++   +   D     V   V  GCG+   G F  V    GL+GLG GKLS+P    +Q
Sbjct: 223 LAKEKFTLTNSD-----VLEDVYFGCGENNQGLFDGVA---GLLGLGPGKLSLP----AQ 282

Query: 396 GLTPYS--FSMC---FGYYGYGRTDFGDIG-PVGQRQTPFN--PASLSYNVTILQIIVTN 455
             T Y+  FS C   F     G   FG  G     + TP +  P++ +Y + I+ I V +
Sbjct: 283 TTTTYNNIFSYCLPSFTSNSTGHLTFGSAGISESVKFTPISSFPSAFNYGIDIIGISVGD 342

Query: 456 RPRNV---HFT---AIIDSGASFTYLTDPFYSIITENVSLATIFQQPNLNF-TMEGGRRF 515
           +   +    F+   AIIDSG  FT L    Y+       L ++F++   ++ +  G   F
Sbjct: 343 KELAITPNSFSTEGAIIDSGTVFTRLPTKVYA------ELRSVFKEKMSSYKSTSGYGLF 402

Query: 516 DFITSYVSVDTDDGPALCLVIVKSTDINVIG 529
           D    +  +DT   P +      ST + + G
Sbjct: 403 DTCYDFTGLDTVTYPTIAFSFAGSTVVELDG 412

BLAST of Chy2G028100 vs. ExPASy Swiss-Prot
Match: Q9M9A8 (Aspartyl protease APCB1 OS=Arabidopsis thaliana OX=3702 GN=APCB1 PE=1 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 7.8e-08
Identity = 63/261 (24.14%), Postives = 103/261 (39.46%), Query Frame = 0

Query: 12  DAKVTLGCGKVQTGKFAN-VTAGNGLIGLGMGKLSVPSFLASQGLTADSFSMCFG--YYG 71
           ++ +  GCG  Q G   N +   +G++GL   K+S+PS LAS+G+ ++    C      G
Sbjct: 313 ESDIVFGCGYDQQGLLLNTLLKTDGILGLSRAKISLPSQLASRGIISNVVGHCLASDLNG 372

Query: 72  YGRIDFG-DIGPV-GQRETP-----------FNPASLSYNVTVLQIIVTNRPTNVHFTAI 131
            G I  G D+ P  G    P                +SY   +L +   N         +
Sbjct: 373 EGYIFMGSDLVPSHGMTWVPMLHDSRLDAYQMQVTKMSYGQGMLSLDGENGRVG---KVL 432

Query: 132 IDTGSSFTYLTDPFYSIIFEKIDADTELELITSDSDFPFEYCYRISLGTIF--------- 191
            DTGSS+TY  +  YS +   +   + LEL   DSD     C+R      F         
Sbjct: 433 FDTGSSYTYFPNQAYSQLVTSLQEVSGLELTRDDSDETLPICWRAKTNFPFSSLSDVKKF 492

Query: 192 -------------------LTPPVNFTMEGGHGPALCLAIVKSTDIN-----VIGSNLLG 224
                              L  P ++ +    G  +CL I+  + ++     ++G   + 
Sbjct: 493 FRPITLQIGSKWLIISRKLLIQPEDYLIISNKG-NVCLGILDGSSVHDGSTIILGDISMR 552

BLAST of Chy2G028100 vs. ExPASy Swiss-Prot
Match: A2ZC67 (Aspartic proteinase Asp1 OS=Oryza sativa subsp. indica OX=39946 GN=ASP1 PE=2 SV=2)

HSP 1 Score: 56.2 bits (134), Expect = 1.5e-06
Identity = 50/169 (29.59%), Postives = 76/169 (44.97%), Query Frame = 0

Query: 313 KSSCPYQTHYLSQNSSSTGYLVQDILHMATDDSQLKPVDVKVTLGCGKVQAGKFSNVTAP 372
           K+ C Y   Y+    SS G L+ D   +   +         +  GCG  Q     NV  P
Sbjct: 112 KNQCHYGIQYV--GGSSIGVLIVDSFSLPASNG---TNPTSIAFGCGYNQGKNNHNVPTP 171

Query: 373 -NGLIGLGMGKLSIPSFLASQG-LTPYSFSMCFGYYGYGRTDFGD--IGPVGQRQTPFNP 432
            NG++GLG GK+++ S L SQG +T +    C    G G   FGD  +   G   +P N 
Sbjct: 172 VNGILGLGRGKVTLLSQLKSQGVITKHVLGHCISSKGKGFLFFGDAKVPTSGVTWSPMNR 231

Query: 433 ASLSYNVT--ILQIIVTNRPRN-VHFTAIIDSGASFTYLT-DPFYSIIT 474
               Y+     LQ    ++P +      I DSGA++TY    P+++ ++
Sbjct: 232 EHKHYSPRQGTLQFNSNSKPISAAPMEVIFDSGATYTYFALQPYHATLS 275

BLAST of Chy2G028100 vs. ExPASy TrEMBL
Match: A0A0A0KGS0 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G507240 PE=3 SV=1)

HSP 1 Score: 593.6 bits (1529), Expect = 9.5e-166
Identity = 301/352 (85.51%), Postives = 311/352 (88.35%), Query Frame = 0

Query: 277 NGKFWSNHYSSNASSTSIHVHCSSSLCELANQCSSNKSSCPYQTHYLSQNSSSTGYLVQD 336
           NGKFW NHYSSNASSTSI V CSSSLCELANQCSSNKSSCPYQTHYLS+NSSS GYLVQD
Sbjct: 146 NGKFWLNHYSSNASSTSIRVPCSSSLCELANQCSSNKSSCPYQTHYLSENSSSAGYLVQD 205

Query: 337 ILHMATDDSQLKPVDVKVTLGCGKVQAGKFSNVTAPNGLIGLGMGKLSIPSFLASQGLTP 396
           ILHMATDDSQLKPVDVKVTLGCGKVQ GKFSNVTAPNGLIGLGMGK+S+PSFLASQGLT 
Sbjct: 206 ILHMATDDSQLKPVDVKVTLGCGKVQTGKFSNVTAPNGLIGLGMGKVSVPSFLASQGLTT 265

Query: 397 YSFSMCFGYYGYGRTDFGDIGPVGQRQTPFNPASLSYNVTILQIIVTNRPRNVHFTAIID 456
            SFSMCFGYYGYGR DFGDIGPVGQR+TPFNPASLSYNVTILQIIVTNRP NVH TAIID
Sbjct: 266 DSFSMCFGYYGYGRIDFGDIGPVGQRETPFNPASLSYNVTILQIIVTNRPTNVHLTAIID 325

Query: 457 SGASFTYLTDPFYSIITEN-----------------------VSLATIFQQPNLNFTMEG 516
           SGASFTYLTDPFYSIITEN                       +SLATIFQQPNLNFTMEG
Sbjct: 326 SGASFTYLTDPFYSIITENMDAAMELERIKSDSDFPFEYCYRLSLATIFQQPNLNFTMEG 385

Query: 517 GRRFDFITSYVSVDTDDGPALCLVIVKSTDINVIGHNFLGGYRIVINREKMTLGWKEVDC 576
           GR+FD ITSYVSVDTDDGPALCL IVKSTDINVIGHNF GGYR+V NREKMTLGWKEVDC
Sbjct: 386 GRKFDVITSYVSVDTDDGPALCLAIVKSTDINVIGHNFFGGYRVVFNREKMTLGWKEVDC 445

Query: 577 DSYDANTSSNDSPPPFRDSSPTTSTPRKSNSTQPSPEIGTGDAMRLNPILSV 606
           DSYDANTSS+DSPPP  DSSPTTSTPRKSNSTQPSPEIG GDAM LNPI+S+
Sbjct: 446 DSYDANTSSDDSPPPSGDSSPTTSTPRKSNSTQPSPEIGAGDAMLLNPIVSL 497

BLAST of Chy2G028100 vs. ExPASy TrEMBL
Match: A0A5A7SLV5 (Aspartyl protease family protein 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G00970 PE=3 SV=1)

HSP 1 Score: 523.1 bits (1346), Expect = 1.6e-144
Identity = 268/353 (75.92%), Postives = 291/353 (82.44%), Query Frame = 0

Query: 277 NGKFWSNHYSSNASSTSIHVHCSSSLCELANQCSSNKSSCPYQTHYLSQNSSSTGYLVQD 336
           N KFW NHYSSNASSTSI V CSSSLCE ANQCSSN+SSCPY+THY SQNSSS GYLVQD
Sbjct: 136 NEKFWLNHYSSNASSTSIRVPCSSSLCEHANQCSSNRSSCPYKTHYPSQNSSSAGYLVQD 195

Query: 337 ILHMATDDSQLKPVDVKVTLGCGKVQAGKFSNVTAPNGLIGLGMGKLSIPSFLASQGLTP 396
           ILHMATDDSQLKPVD KVTLGCGKVQ GKF+N TA NGLIGLGMGKLS+PSFLASQGLT 
Sbjct: 196 ILHMATDDSQLKPVDAKVTLGCGKVQTGKFANFTAANGLIGLGMGKLSVPSFLASQGLTA 255

Query: 397 YSFSMCFGYYGYGRTDFGDIGPVGQRQTPFNPASLSYNVTILQIIVTNRPRNVHFTAIID 456
            SFSMCFGYYGYGR DFGDIG VGQR+TP NP SLSYNVTILQIIVTNRP NVHFTAIID
Sbjct: 256 DSFSMCFGYYGYGRIDFGDIGSVGQRETPLNPTSLSYNVTILQIIVTNRPTNVHFTAIID 315

Query: 457 SGASFTYLTDPFYSIITE-----------------------NVSLATIFQQPNLNFTMEG 516
           +G+SFTYLTDPFYSII++                        +S+ TIF QP +NFTME 
Sbjct: 316 AGSSFTYLTDPFYSIISDKMDEAIELERIASDSDFPFEYCYQLSMGTIFLQPTMNFTMED 375

Query: 517 GRRFDFITSYVSVDTDDGPALCLVIVKSTDINVIGHNFLGGYRIVINREKMTLGWKE-VD 576
           GR+FD I+SYV ++TDDG ALCL IVKSTDINVIG N L GYR+V NREKMTLGWKE VD
Sbjct: 376 GRKFDVISSYVMINTDDGIALCLAIVKSTDINVIGSNLLSGYRVVFNREKMTLGWKEVVD 435

Query: 577 CDSYDANTSSNDSPPPFRDSSPTTSTPRKSNSTQPSPEIGTGDAMRLNPILSV 606
           CD+Y A+T S +SPPPF  S P TS+PRKSNSTQPSPEIGTGDAMRLNPI+S+
Sbjct: 436 CDNYGADTPSGESPPPFGGSPPPTSSPRKSNSTQPSPEIGTGDAMRLNPIVSL 488

BLAST of Chy2G028100 vs. ExPASy TrEMBL
Match: A0A1S3B2V8 (aspartyl protease family protein 1-like OS=Cucumis melo OX=3656 GN=LOC103485160 PE=3 SV=1)

HSP 1 Score: 522.3 bits (1344), Expect = 2.7e-144
Identity = 268/353 (75.92%), Postives = 291/353 (82.44%), Query Frame = 0

Query: 277 NGKFWSNHYSSNASSTSIHVHCSSSLCELANQCSSNKSSCPYQTHYLSQNSSSTGYLVQD 336
           N KFW NHYSSNASSTSI V CSSSLCE ANQCSSN+SSCPY+THY SQNSSS GYLVQD
Sbjct: 146 NEKFWLNHYSSNASSTSIRVPCSSSLCEHANQCSSNRSSCPYKTHYPSQNSSSAGYLVQD 205

Query: 337 ILHMATDDSQLKPVDVKVTLGCGKVQAGKFSNVTAPNGLIGLGMGKLSIPSFLASQGLTP 396
           ILHMATDDSQLKPVD KVTLGCGKVQ GKF+N TA NGLIGLGMGKLS+PSFLASQGLT 
Sbjct: 206 ILHMATDDSQLKPVDAKVTLGCGKVQTGKFANFTAANGLIGLGMGKLSVPSFLASQGLTA 265

Query: 397 YSFSMCFGYYGYGRTDFGDIGPVGQRQTPFNPASLSYNVTILQIIVTNRPRNVHFTAIID 456
            SFSMCFGYYG GR DFGDIG VGQR+TP NP SLSYNVTILQIIVTNRP NVHFTAIID
Sbjct: 266 DSFSMCFGYYGNGRIDFGDIGSVGQRETPLNPTSLSYNVTILQIIVTNRPTNVHFTAIID 325

Query: 457 SGASFTYLTDPFYSIITE-----------------------NVSLATIFQQPNLNFTMEG 516
           +G+SFTYLTDPFYSII++                        +S+ TIF QP +NFTMEG
Sbjct: 326 TGSSFTYLTDPFYSIISDKMDEAIELERIASDSDFPFEYCYQLSMGTIFLQPTMNFTMEG 385

Query: 517 GRRFDFITSYVSVDTDDGPALCLVIVKSTDINVIGHNFLGGYRIVINREKMTLGWKE-VD 576
           GR+FD I+SYV ++TDDG ALCL IVKSTDINVIG N L GYR+V NREKMTLGWKE VD
Sbjct: 386 GRKFDVISSYVMINTDDGIALCLAIVKSTDINVIGSNLLSGYRVVFNREKMTLGWKEVVD 445

Query: 577 CDSYDANTSSNDSPPPFRDSSPTTSTPRKSNSTQPSPEIGTGDAMRLNPILSV 606
           CD+Y A+T S +SPPPF  S P TS+PRKSNSTQPSPEIGTGDAMRLNPI+S+
Sbjct: 446 CDNYGADTPSGESPPPFGGSPPPTSSPRKSNSTQPSPEIGTGDAMRLNPIVSL 498

BLAST of Chy2G028100 vs. ExPASy TrEMBL
Match: W9SCT8 (Aspartic proteinase-like protein 1 OS=Morus notabilis OX=981085 GN=L484_026627 PE=3 SV=1)

HSP 1 Score: 410.2 bits (1053), Expect = 1.5e-110
Identity = 281/740 (37.97%), Postives = 375/740 (50.68%), Query Frame = 0

Query: 4   DDSQLKPVDAKVTLGCGKVQTGKFANVTAGNGLIGLGMGKLSVPSFLASQGLTADSFSMC 63
           +D   + V+A VT GCG+VQ+G F +V A NGL GLGM K+SVPS L+ +G  A+SFSMC
Sbjct: 177 EDKHEELVEAYVTFGCGQVQSGSFLDVAAPNGLFGLGMEKISVPSILSKEGFIANSFSMC 236

Query: 64  FGYYGYGRIDFGDIGPVGQRETPF--NPASLSYNVTVLQIIVTNRPTNVHFTAIIDTGSS 123
           FG  G GRI FGD G   Q ETPF  NP+  +YN+TV QI V     +  F+A+ D+G+S
Sbjct: 237 FGQDGIGRISFGDKGSPDQDETPFNLNPSHPTYNITVTQIRVGTSLFDADFSALFDSGTS 296

Query: 124 FTYLTDPFYSIIFEKIDADTELELITSDSDFPFEYCYRIS-LGTIFLTPPVNFTMEGG-- 183
           FTYL +P Y+ + E   +  +     +DS  PFEYCY +S      L P ++ TM+GG  
Sbjct: 297 FTYLVEPIYTRLSESFHSQVQDSRRPTDSRIPFEYCYDMSPEANSSLIPSLSLTMKGGCQ 356

Query: 184 ---HGPAL----------CLAIVKSTDINVIGSNLLGGYRIVFNREKMTLGWK-----EV 243
              + P +          CLA+VKS ++N+IG N + GYR+VF+REK+ LGWK     ++
Sbjct: 357 FAVYDPIIVISTQNEIVYCLAVVKSAELNIIGQNFMTGYRVVFDREKLVLGWKKSDCYDI 416

Query: 244 EDCDSYVPNTPSDDSPP---------------------------QSRDSPPAN-----ST 303
           ED +++     S   PP                             R S P         
Sbjct: 417 EDYNNFPAKPNSTTVPPAVAAGLVVVLGWGIGGCSGFGTFGCDIHHRLSDPVKGFLGFDA 476

Query: 304 PRKSNSTQPSPKIVAGDAML---------------------------------------- 363
           P K  S      +V GD ++                                        
Sbjct: 477 PEK-GSLDYYKVLVHGDRLIRGRHLASDDQTSSPLTFYDGNATYRLATFGFLHYANITVG 536

Query: 364 ---LNPIVSLCNGK--FW---------------------SNHYSSNASSTSIHVHCSSSL 423
               + +V+L  G   FW                      + YS N SSTS  V C+S+L
Sbjct: 537 SPSSSYLVALDTGSDLFWLPCDCTSCVSGIKFSDGEILKFSIYSPNTSSTSSKVSCNSTL 596

Query: 424 CE-LANQCSSNKSSCPYQTHYLSQNSSSTGYLVQDILHMATDDSQLKPVDVKVTLGCGKV 483
           CE    QC S  S+CPYQ  YLS  ++STGYLV+D+LH+ +DD QLK VD ++T GCGKV
Sbjct: 597 CEQWRRQCPSETSTCPYQVVYLSNGTASTGYLVEDVLHLKSDDDQLKDVDARITFGCGKV 656

Query: 484 QAGKFSNVTAPNGLIGLGMGKLSIPSFLASQGLTPYSFSMCFGYYGYGRTDFGDIGPVGQ 543
           Q G F +  APNGLIGLG+  +S+PS LA +G T  SFSMCFG    GR  FGD G   Q
Sbjct: 657 QTGTFLDGAAPNGLIGLGIEDISVPSILAKEGQTSNSFSMCFGSDDLGRIRFGDNGSADQ 716

Query: 544 RQTPFN--PASLSYNVTILQIIVTNRPRNVHFTAIIDSGASFTYLTDPFYSIITENVS-L 595
            +TPFN   +  SYN+TI QI V     ++ F AI D+G SFTYL DP Y+ ITE+ + L
Sbjct: 717 SETPFNLRQSHPSYNITITQITVGGTVADLEFHAIFDTGTSFTYLNDPAYTQITESFNYL 776

BLAST of Chy2G028100 vs. ExPASy TrEMBL
Match: A0A371HV19 (Aspartyl protease family protein 1 (Fragment) OS=Mucuna pruriens OX=157652 GN=APF1 PE=3 SV=1)

HSP 1 Score: 408.7 bits (1049), Expect = 4.3e-110
Identity = 271/675 (40.15%), Postives = 360/675 (53.33%), Query Frame = 0

Query: 1   MATDDSQLKPVDAKVTLGCGKVQTGKFANVTAGNGLIGLGMGKLSVPSFLASQGLTADSF 60
           + TDD Q K  DA +T GCG+VQTG      A NGL GLGM  +SVPS LA +GL ++SF
Sbjct: 217 LITDDDQTKDADAIITFGCGQVQTGVLLKGEAPNGLFGLGMDNISVPSILAKEGLISNSF 276

Query: 61  SMCFGYYGYGRIDFGDIGPVGQRETPFNPASL--SYNVTVLQIIVTNRPTNVHFTAIIDT 120
           SMCFG  G GRI FGD G   Q +TPFN  +L  +YN+T+ QIIV     ++ F AI D+
Sbjct: 277 SMCFGSNGSGRIAFGDTGVSDQGKTPFNLKTLHPTYNITITQIIVGGNAADLEFHAIFDS 336

Query: 121 GSSFTYLTDPFYSIIFEKIDA---DTELELITSDSDFPFEYCYRISLG-TIFLTPPVNFT 180
           G+SFTY+ DP Y+++ +K  +   +      + DS+ PFEYCY IS   TI   P +N T
Sbjct: 337 GTSFTYINDPAYTLLADKFSSLVKEKRHSFQSPDSNIPFEYCYEISPNQTIIEVPLLNLT 396

Query: 181 MEGG----------------HGPALCLAIVKSTDINVIGSNLLGGYRIVFNREKMTLGWK 240
           M+GG                 G  LCL I+KS  +N+IG N + GY+IVF+RE M LGWK
Sbjct: 397 MKGGDDYYVSDPTIQVSSEDEGNLLCLGILKSDSVNIIGQNFMTGYKIVFDRENMKLGWK 456

Query: 241 EVEDCD---------------------SYVPNTPSDDSPPQSRDSPPANSTP---RKSNS 300
           E  +C+                      Y       D   ++R     + TP      N 
Sbjct: 457 E-SNCEFSDPVKGIMGNILHLPQKGTSQYYAAMAHRDRFFKARSLAADSHTPLTFAAGNH 516

Query: 301 TQPSPKIVAGDAMLLNPIVSLCNGKFWSNHYSSNASSTSIHVHCSSSLCELANQCSSNKS 360
           T   P +      L    VS+ N     N Y  + SSTS  V C++S      QC S+ S
Sbjct: 517 TLSIPSL----GFLHYANVSIVN----LNAYDLDKSSTSKKVSCNNSSFCKQKQCPSSGS 576

Query: 361 SCPYQTHYLSQNSSSTGYLVQDILHMATDDSQLKPVDVKVTLGCGKVQAGKFSNVTAPNG 420
           SC YQ  YLS ++SS+G+LV+D+LH+ TDD Q K  D  VT GCG+VQ G F    A NG
Sbjct: 577 SCRYQVDYLSNDTSSSGFLVEDVLHLITDDDQTKDADTFVTFGCGQVQTGVFLRGAALNG 636

Query: 421 LIGLGMGKLSIPSFLASQGLTPYSFSMCFGYYGYGRTDFGDIGPVGQRQTPFNPASL--S 480
           L GLGMG +S+PS LA +GL   SFSMCFGY   GR  FGD G   Q +TPFN  +L  +
Sbjct: 637 LFGLGMGNISVPSILAKEGLISNSFSMCFGYDRVGRITFGDTGGPDQGKTPFNLGTLHPT 696

Query: 481 YNVTILQIIVTNRPRNVHFTAIIDSGASFTYLTDPFYSIITENVSL-------------- 540
           YN+TI QIIV  R  ++ F AI DSG SFTY+ DP Y++++E  +               
Sbjct: 697 YNITITQIIVEGRVADLEFHAIFDSGTSFTYINDPAYTLLSEKFNSQVKAKRHSSQSPVS 756

Query: 541 -------------ATIFQQPNLNFTMEGGRRFDFITSYVSV------DTDDGPALCLVIV 589
                         TI + P LN TM+GG  +     YV V        D+G   CL + 
Sbjct: 757 NIPFEYCYEISPNQTIIEFPVLNLTMKGGDDY-----YVKVPIELLYSEDEGNLFCLGMH 816

BLAST of Chy2G028100 vs. NCBI nr
Match: XP_004135019.1 (aspartyl protease family protein 1 [Cucumis sativus] >KGN48950.1 hypothetical protein Csa_004140 [Cucumis sativus])

HSP 1 Score: 587 bits (1514), Expect = 5.94e-203
Identity = 301/352 (85.51%), Postives = 311/352 (88.35%), Query Frame = 0

Query: 277 NGKFWSNHYSSNASSTSIHVHCSSSLCELANQCSSNKSSCPYQTHYLSQNSSSTGYLVQD 336
           NGKFW NHYSSNASSTSI V CSSSLCELANQCSSNKSSCPYQTHYLS+NSSS GYLVQD
Sbjct: 146 NGKFWLNHYSSNASSTSIRVPCSSSLCELANQCSSNKSSCPYQTHYLSENSSSAGYLVQD 205

Query: 337 ILHMATDDSQLKPVDVKVTLGCGKVQAGKFSNVTAPNGLIGLGMGKLSIPSFLASQGLTP 396
           ILHMATDDSQLKPVDVKVTLGCGKVQ GKFSNVTAPNGLIGLGMGK+S+PSFLASQGLT 
Sbjct: 206 ILHMATDDSQLKPVDVKVTLGCGKVQTGKFSNVTAPNGLIGLGMGKVSVPSFLASQGLTT 265

Query: 397 YSFSMCFGYYGYGRTDFGDIGPVGQRQTPFNPASLSYNVTILQIIVTNRPRNVHFTAIID 456
            SFSMCFGYYGYGR DFGDIGPVGQR+TPFNPASLSYNVTILQIIVTNRP NVH TAIID
Sbjct: 266 DSFSMCFGYYGYGRIDFGDIGPVGQRETPFNPASLSYNVTILQIIVTNRPTNVHLTAIID 325

Query: 457 SGASFTYLTDPFYSIITENV-----------------------SLATIFQQPNLNFTMEG 516
           SGASFTYLTDPFYSIITEN+                       SLATIFQQPNLNFTMEG
Sbjct: 326 SGASFTYLTDPFYSIITENMDAAMELERIKSDSDFPFEYCYRLSLATIFQQPNLNFTMEG 385

Query: 517 GRRFDFITSYVSVDTDDGPALCLVIVKSTDINVIGHNFLGGYRIVINREKMTLGWKEVDC 576
           GR+FD ITSYVSVDTDDGPALCL IVKSTDINVIGHNF GGYR+V NREKMTLGWKEVDC
Sbjct: 386 GRKFDVITSYVSVDTDDGPALCLAIVKSTDINVIGHNFFGGYRVVFNREKMTLGWKEVDC 445

Query: 577 DSYDANTSSNDSPPPFRDSSPTTSTPRKSNSTQPSPEIGTGDAMRLNPILSV 605
           DSYDANTSS+DSPPP  DSSPTTSTPRKSNSTQPSPEIG GDAM LNPI+S+
Sbjct: 446 DSYDANTSSDDSPPPSGDSSPTTSTPRKSNSTQPSPEIGAGDAMLLNPIVSL 497

BLAST of Chy2G028100 vs. NCBI nr
Match: KAA0025643.1 (aspartyl protease family protein 1-like [Cucumis melo var. makuwa] >TYK12516.1 aspartyl protease family protein 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 517 bits (1331), Expect = 2.25e-175
Identity = 268/353 (75.92%), Postives = 291/353 (82.44%), Query Frame = 0

Query: 277 NGKFWSNHYSSNASSTSIHVHCSSSLCELANQCSSNKSSCPYQTHYLSQNSSSTGYLVQD 336
           N KFW NHYSSNASSTSI V CSSSLCE ANQCSSN+SSCPY+THY SQNSSS GYLVQD
Sbjct: 136 NEKFWLNHYSSNASSTSIRVPCSSSLCEHANQCSSNRSSCPYKTHYPSQNSSSAGYLVQD 195

Query: 337 ILHMATDDSQLKPVDVKVTLGCGKVQAGKFSNVTAPNGLIGLGMGKLSIPSFLASQGLTP 396
           ILHMATDDSQLKPVD KVTLGCGKVQ GKF+N TA NGLIGLGMGKLS+PSFLASQGLT 
Sbjct: 196 ILHMATDDSQLKPVDAKVTLGCGKVQTGKFANFTAANGLIGLGMGKLSVPSFLASQGLTA 255

Query: 397 YSFSMCFGYYGYGRTDFGDIGPVGQRQTPFNPASLSYNVTILQIIVTNRPRNVHFTAIID 456
            SFSMCFGYYGYGR DFGDIG VGQR+TP NP SLSYNVTILQIIVTNRP NVHFTAIID
Sbjct: 256 DSFSMCFGYYGYGRIDFGDIGSVGQRETPLNPTSLSYNVTILQIIVTNRPTNVHFTAIID 315

Query: 457 SGASFTYLTDPFYSIITENV-----------------------SLATIFQQPNLNFTMEG 516
           +G+SFTYLTDPFYSII++ +                       S+ TIF QP +NFTME 
Sbjct: 316 AGSSFTYLTDPFYSIISDKMDEAIELERIASDSDFPFEYCYQLSMGTIFLQPTMNFTMED 375

Query: 517 GRRFDFITSYVSVDTDDGPALCLVIVKSTDINVIGHNFLGGYRIVINREKMTLGWKEV-D 576
           GR+FD I+SYV ++TDDG ALCL IVKSTDINVIG N L GYR+V NREKMTLGWKEV D
Sbjct: 376 GRKFDVISSYVMINTDDGIALCLAIVKSTDINVIGSNLLSGYRVVFNREKMTLGWKEVVD 435

Query: 577 CDSYDANTSSNDSPPPFRDSSPTTSTPRKSNSTQPSPEIGTGDAMRLNPILSV 605
           CD+Y A+T S +SPPPF  S P TS+PRKSNSTQPSPEIGTGDAMRLNPI+S+
Sbjct: 436 CDNYGADTPSGESPPPFGGSPPPTSSPRKSNSTQPSPEIGTGDAMRLNPIVSL 488

BLAST of Chy2G028100 vs. NCBI nr
Match: XP_008440865.1 (PREDICTED: aspartyl protease family protein 1-like [Cucumis melo])

HSP 1 Score: 516 bits (1329), Expect = 6.35e-175
Identity = 268/353 (75.92%), Postives = 291/353 (82.44%), Query Frame = 0

Query: 277 NGKFWSNHYSSNASSTSIHVHCSSSLCELANQCSSNKSSCPYQTHYLSQNSSSTGYLVQD 336
           N KFW NHYSSNASSTSI V CSSSLCE ANQCSSN+SSCPY+THY SQNSSS GYLVQD
Sbjct: 146 NEKFWLNHYSSNASSTSIRVPCSSSLCEHANQCSSNRSSCPYKTHYPSQNSSSAGYLVQD 205

Query: 337 ILHMATDDSQLKPVDVKVTLGCGKVQAGKFSNVTAPNGLIGLGMGKLSIPSFLASQGLTP 396
           ILHMATDDSQLKPVD KVTLGCGKVQ GKF+N TA NGLIGLGMGKLS+PSFLASQGLT 
Sbjct: 206 ILHMATDDSQLKPVDAKVTLGCGKVQTGKFANFTAANGLIGLGMGKLSVPSFLASQGLTA 265

Query: 397 YSFSMCFGYYGYGRTDFGDIGPVGQRQTPFNPASLSYNVTILQIIVTNRPRNVHFTAIID 456
            SFSMCFGYYG GR DFGDIG VGQR+TP NP SLSYNVTILQIIVTNRP NVHFTAIID
Sbjct: 266 DSFSMCFGYYGNGRIDFGDIGSVGQRETPLNPTSLSYNVTILQIIVTNRPTNVHFTAIID 325

Query: 457 SGASFTYLTDPFYSIITENV-----------------------SLATIFQQPNLNFTMEG 516
           +G+SFTYLTDPFYSII++ +                       S+ TIF QP +NFTMEG
Sbjct: 326 TGSSFTYLTDPFYSIISDKMDEAIELERIASDSDFPFEYCYQLSMGTIFLQPTMNFTMEG 385

Query: 517 GRRFDFITSYVSVDTDDGPALCLVIVKSTDINVIGHNFLGGYRIVINREKMTLGWKEV-D 576
           GR+FD I+SYV ++TDDG ALCL IVKSTDINVIG N L GYR+V NREKMTLGWKEV D
Sbjct: 386 GRKFDVISSYVMINTDDGIALCLAIVKSTDINVIGSNLLSGYRVVFNREKMTLGWKEVVD 445

Query: 577 CDSYDANTSSNDSPPPFRDSSPTTSTPRKSNSTQPSPEIGTGDAMRLNPILSV 605
           CD+Y A+T S +SPPPF  S P TS+PRKSNSTQPSPEIGTGDAMRLNPI+S+
Sbjct: 446 CDNYGADTPSGESPPPFGGSPPPTSSPRKSNSTQPSPEIGTGDAMRLNPIVSL 498

BLAST of Chy2G028100 vs. NCBI nr
Match: XP_038882976.1 (aspartyl protease family protein 1-like [Benincasa hispida])

HSP 1 Score: 436 bits (1122), Expect = 1.21e-143
Identity = 233/358 (65.08%), Postives = 269/358 (75.14%), Query Frame = 0

Query: 279 KFWSNHYSSNASSTSIHVHCSSSLCELANQCSSNKSSCPYQTHYLSQNSSSTGYLVQDIL 338
           KFW NHYS N S+TS +V CS+SLCEL NQC+SNKS+CPY+TH+LS N+SS GYLVQDIL
Sbjct: 148 KFWLNHYSPNDSTTSTNVPCSNSLCELTNQCTSNKSTCPYKTHHLSPNTSSAGYLVQDIL 207

Query: 339 HMATDDSQLKPVDVKVTLGCGKVQAGKFSNVTAPNGLIGLGMGKLSIPSFLASQGLTPYS 398
           H+AT+DSQ+KPVD+KVTLGCGKVQ G FSN+ APNGLIGLGMGKLS+PSFLAS GL P S
Sbjct: 208 HLATNDSQVKPVDMKVTLGCGKVQTGLFSNIAAPNGLIGLGMGKLSVPSFLASHGL-PDS 267

Query: 399 FSMCFGYYGYGRTDFGDIGPVGQRQTPFNPASLSYNVTILQIIVTNRPRNVHFTAIIDSG 458
           FSMCF Y G GR D+GD+GP+GQR+TP NP SL YNVTI+QIIV  RP NV FTAIID+G
Sbjct: 268 FSMCFAYDGLGRIDYGDVGPLGQRETPLNPMSLYYNVTIIQIIVVTRPTNVEFTAIIDTG 327

Query: 459 ASFTYLTDPFYSIITENVSLA-----------------------TIFQQPNLNFTMEGGR 518
           +SFTYL DP YS+IT+ +  A                       TIF  PNLNFTME G 
Sbjct: 328 SSFTYLADPIYSLITKKMDAAMKLDRITFDSDYPFEYCYQLPLKTIFLSPNLNFTMESGY 387

Query: 519 RFDFITSYVSVDTDD--GPALCLVIVKSTDINVIGHNFLGGYRIVINREKMTLGWKEV-D 578
            F  IT+YVS+ TDD  G A+CL I+KST+IN+IG N L GYRIV +REKM LGWKEV D
Sbjct: 388 DFGAITTYVSIPTDDMGGYAVCLAILKSTNINIIGQNLLTGYRIVFDREKMVLGWKEVED 447

Query: 579 CDSYDANTSSNDSPP-----PFRDSSPTTSTPRKSNSTQPSPEIGTGDAMRLNPILSV 605
           C+SY   T S +SPP     P  DS P TSTPRKSNSTQP PEIG GDA  LNP++S+
Sbjct: 448 CNSYGVGTPSGNSPPANEYPPSGDSPPATSTPRKSNSTQPLPEIGAGDATPLNPLVSL 504

BLAST of Chy2G028100 vs. NCBI nr
Match: KAG7034068.1 (Aspartyl protease family protein 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 448 bits (1152), Expect = 1.74e-143
Identity = 305/703 (43.39%), Postives = 376/703 (53.49%), Query Frame = 0

Query: 1   MATDDSQLKPVDAKVTLGCGKVQTGKFANVTAGNGLIGLGMGKLSVPSFLASQGLTADSF 60
           + TDDSQLKPV AK+T GCGKVQTG+FA   A NGLIGLGM ++SVPSFLA+QGL +DSF
Sbjct: 206 LITDDSQLKPVKAKITFGCGKVQTGRFARHAAPNGLIGLGMERISVPSFLANQGLISDSF 265

Query: 61  SMCFGYYGYGRIDFGDIGPVGQRETPFN--PASLSYNVTVLQIIVTNRPTNVHFTAIIDT 120
           SMCFG+   GRIDFGD G   Q+ETP N  P+   YNVT+ QIIV  +  +V FTAI D 
Sbjct: 266 SMCFGHDEIGRIDFGDTGTPDQKETPINSNPSFPHYNVTITQIIVGGKTNDVQFTAIFDG 325

Query: 121 GSSFTYLTDPFYSIIFEKIDADTELEL---ITSDSDFPFEYCYRISLGTIFL-------- 180
           G+S    T     + F     D  + L   +T   D      Y     T +         
Sbjct: 326 GASLYAETIDCPVVNFTMKGGDDFIPLGQFLTLSIDDADTRRYNNGAATPYGYTTPFDSP 385

Query: 181 ----------TPPVNFTMEGGHG-PAL--------------------CLAIVKSTDINV- 240
                     +PP   T  G  G P +                     LA+V S   N+ 
Sbjct: 386 PTDKSPPSDDSPPAPVTPGGSTGLPNIEVGVATRLNPLTSVFVAVLAILAVVVSLKFNIH 445

Query: 241 ----------------IGSNLLGGYRIVFNREKMTLGWKEVEDCDSYVPNTPSDDSPPQS 300
                           +  +  G Y  + +R+++ L  + +    S  P T S D     
Sbjct: 446 RRFSDSIKGILNSDGLLEKHTPGYYAAMVHRDRL-LHARRLATSKSETPLTFSYD----- 505

Query: 301 RDSPPANSTPRK--------SNSTQPSPKI-------VAGDAMLLNPIVSLCNGKFWS-- 360
                 N T R         +N +  +P++          D   L    S C     +  
Sbjct: 506 ------NHTYRYGGLGDFYYANISVGTPELGFLVALDTGSDLSWLPCECSKCRTSVGTSD 565

Query: 361 ------NHYSSNASSTSIHVHCSSSLCELANQCSSNKSSCPYQTHYLSQNSSSTGYLVQD 420
                 NHYS NAS+TS  V CS+SLCELA+QC SNKSSCPY+ H  S N+SS+GYLV+D
Sbjct: 566 GEDLTLNHYSPNASTTSKSVACSNSLCELADQCPSNKSSCPYKIHDASPNTSSSGYLVED 625

Query: 421 ILHMATDDSQLKPVDVKVTLGCGKVQAGKFSNVTAPNGLIGLGMGKLSIPSFLASQGLTP 480
           ILH+AT+DSQ KPVD K+T GCGKVQ G FS++ A NGL+GLGMGK+S+PS LASQ LTP
Sbjct: 626 ILHLATNDSQSKPVDAKITFGCGKVQTGIFSSI-AINGLLGLGMGKISVPSLLASQELTP 685

Query: 481 YSFSMCFGYYGYGRTDFGDIGPVGQRQTPFNPASLSYNVTILQIIVTNRPRNVHFTAIID 540
            SFSMCFGY GYG  DFGDIGP  QR+T  NP S SYNVTI+QI V  +P NV FT I D
Sbjct: 686 DSFSMCFGYDGYGNIDFGDIGPSDQRETSLNPHSSSYNVTIIQITVAGKPNNVEFTTIFD 745

Query: 541 SGASFTYLTDPFYSIITENVSLATIFQQPNLNFTMEGGRRFDFITSYVSVDTDDGPALCL 600
           SG S+TYL+DP Y+ +T+N                                   G A+CL
Sbjct: 746 SGFSYTYLSDPIYTFVTQN--------------------------------DKGGYAICL 805

Query: 601 VIVKSTDINVIGHNFLGGYRIVINREKMTLGWKEVDC-----------------DSYDAN 602
            I KSTDIN+ G NF+ G+RIV NREKM LGW EV+C                 +S  +N
Sbjct: 806 TIAKSTDINLFGLNFMAGHRIVFNREKMVLGWNEVNCSVFLHLKTSLRFDNGADNSPPSN 863

BLAST of Chy2G028100 vs. TAIR 10
Match: AT2G17760.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 250.0 bits (637), Expect = 4.9e-66
Identity = 145/345 (42.03%), Postives = 194/345 (56.23%), Query Frame = 0

Query: 283 NHYSSNASSTSIHVHCSSSLCELANQCSSNKSSCPYQTHYLSQNSSSTGYLVQDILHMAT 342
           N YS NASSTS  V C+S+LC   ++C+S +S CPYQ  YLS  +SSTG LV+D+LH+ +
Sbjct: 152 NIYSPNASSTSTKVPCNSTLCTRGDRCASPESDCPYQIRYLSNGTSSTGVLVEDVLHLVS 211

Query: 343 DDSQLKPVDVKVTLGCGKVQAGKFSNVTAPNGLIGLGMGKLSIPSFLASQGLTPYSFSMC 402
           +D   K +  +VT GCG+VQ G F +  APNGL GLG+  +S+PS LA +G+   SFSMC
Sbjct: 212 NDKSSKAIPARVTFGCGQVQTGVFHDGAAPNGLFGLGLEDISVPSVLAKEGIAANSFSMC 271

Query: 403 FGYYGYGRTDFGDIGPVGQRQTPFN--PASLSYNVTILQIIVTNRPRNVHFTAIIDSGAS 462
           FG  G GR  FGD G V QR+TP N      +YN+T+ +I V     ++ F A+ DSG S
Sbjct: 272 FGNDGAGRISFGDKGSVDQRETPLNIRQPHPTYNITVTKISVGGNTGDLEFDAVFDSGTS 331

Query: 463 FTYLTDPFYSIITENVSLATI-------------------------FQQPNLNFTMEGGR 522
           FTYLTD  Y++I+E+ +   +                         FQ P +N TM+GG 
Sbjct: 332 FTYLTDAAYTLISESFNSLALDKRYQTTDSELPFEYCYALSPNKDSFQYPAVNLTMKGGS 391

Query: 523 RFDFITSYVSVDTDDGPALCLVIVKSTDINVIGHNFLGGYRIVINREKMTLGWKEVDCDS 582
            +      V +   D    CL I+K  DI++IG NF+ GYR+V +REK+ LGWKE DC  
Sbjct: 392 SYPVYHPLVVIPMKDTDVYCLAIMKIEDISIIGQNFMTGYRVVFDREKLILGWKESDC-- 451

Query: 583 YDANTSSNDSPPPFRDSS---PTTS-TPRKSNSTQPSPEIGTGDA 597
           Y   TS+   P     SS   P +S  P  +N     P   T  A
Sbjct: 452 YTGETSARTLPSNRSSSSARPPASSFDPEATNIPSQRPNTSTTSA 494

BLAST of Chy2G028100 vs. TAIR 10
Match: AT4G35880.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 236.1 bits (601), Expect = 7.4e-62
Identity = 122/304 (40.13%), Postives = 181/304 (59.54%), Query Frame = 0

Query: 285 YSSNASSTSIHVHCSSSLCELANQCSSNKSSCPYQTHYLSQNSSSTGYLVQDILHMATDD 344
           Y+   S+T+  V C++SLC   NQC    S+CPY   Y+S  +S++G L++D++H+ T+D
Sbjct: 157 YNPKVSTTNKKVTCNNSLCAQRNQCLGTFSTCPYMVSYVSAQTSTSGILMEDVMHLTTED 216

Query: 345 SQLKPVDVKVTLGCGKVQAGKFSNVTAPNGLIGLGMGKLSIPSFLASQGLTPYSFSMCFG 404
              + V+  VT GCG+VQ+G F ++ APNGL GLGM K+S+PS LA +GL   SFSMCFG
Sbjct: 217 KNPERVEAYVTFGCGQVQSGSFLDIAAPNGLFGLGMEKISVPSVLAREGLVADSFSMCFG 276

Query: 405 YYGYGRTDFGDIGPVGQRQTPF--NPASLSYNVTILQIIVTNRPRNVHFTAIIDSGASFT 464
           + G GR  FGD G   Q +TPF  NP+  +YN+T+ ++ V     +  FTA+ D+G SFT
Sbjct: 277 HDGVGRISFGDKGSSDQEETPFNLNPSHPNYNITVTRVRVGTTLIDDEFTALFDTGTSFT 336

Query: 465 YLTDPFYSIITENV------------------------SLATIFQQPNLNFTMEGGRRFD 524
           YL DP Y+ ++E+                         + A     P+L+ TM+G   F 
Sbjct: 337 YLVDPMYTTVSESFHSQAQDKRHSPDSRIPFEYCYDMSNDANASLIPSLSLTMKGNSHFT 396

Query: 525 FITSYVSVDTDDGPALCLVIVKSTDINVIGHNFLGGYRIVINREKMTLGWKEVDC-DSYD 562
                + + T+     CL IVKS+++N+IG N++ GYR+V +REK+ L WK+ DC D  +
Sbjct: 397 INDPIIVISTEGELVYCLAIVKSSELNIIGQNYMTGYRVVFDREKLVLAWKKFDCYDIEE 456

BLAST of Chy2G028100 vs. TAIR 10
Match: AT3G51330.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 213.8 bits (543), Expect = 3.9e-55
Identity = 143/368 (38.86%), Postives = 190/368 (51.63%), Query Frame = 0

Query: 283 NHYSSNASSTSIHVHCSSSLCELANQCSSNKSSCPYQTHYLSQNSSSTGYLVQDILHMAT 342
           N YS N SSTS  + CS   C  +++CSS  SSCPYQ  YLS+++ +TG L +D+LH+ T
Sbjct: 152 NLYSPNTSSTSSSIRCSDDRCFGSSRCSSPASSCPYQIQYLSKDTFTTGTLFEDVLHLVT 211

Query: 343 DDSQLKPVDVKVTLGCGKVQAGKFSNVTAPNGLIGLGMGKLSIPSFLASQGLTPYSFSMC 402
           +D  L+PV   +TLGCGK Q G   +  A NGL+GLG+   S+PS LA   +T  SFSMC
Sbjct: 212 EDEGLEPVKANITLGCGKNQTGFLQSSAAVNGLLGLGLKDYSVPSILAKAKITANSFSMC 271

Query: 403 FGYY--GYGRTDFGDIGPVGQRQTPFNPA--SLSYNVTILQIIVTNRPRNVHFTAIIDSG 462
           FG      GR  FGD G   Q +TP  P   S +Y V++ ++ V      V   A+ D+G
Sbjct: 272 FGNIIDVVGRISFGDKGYTDQMETPLLPTEPSPTYAVSVTEVSVGGDAVGVQLLALFDTG 331

Query: 463 ASFTYLTDPFYSIITENVS------------------------LATIFQQPNLNFTMEGG 522
            SFT+L +P Y +IT+                             T    P +  T EGG
Sbjct: 332 TSFTHLLEPEYGLITKAFDDHVTDKRRPIDPELPFEFCYDLSPNKTTILFPRVAMTFEGG 391

Query: 523 RRFDFITSYVSVDTDDGPAL-CLVIVKSTD--INVIGHNFLGGYRIVINREKMTLGWKEV 582
            +         V  +D  A+ CL I+KS D  IN+IG NF+ GYRIV +RE+M LGWK  
Sbjct: 392 SQMFLRNPLFIVWNEDNSAMYCLGILKSVDFKINIIGQNFMSGYRIVFDRERMILGWKRS 451

Query: 583 DCDSYDANTSSNDSPPPFRDSSPTTSTPRKSNSTQPS----PEI---------GTGDAMR 607
           DC   ++  S+   PP     SP+ STP  S    P+    P+I         GTG A  
Sbjct: 452 DCFEDESLESTTPPPPETEAPSPSASTPLPSLLPPPAAATPPQIDPRNSTRNSGTGTAAN 511

BLAST of Chy2G028100 vs. TAIR 10
Match: AT3G51350.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 201.4 bits (511), Expect = 2.0e-51
Identity = 138/368 (37.50%), Postives = 189/368 (51.36%), Query Frame = 0

Query: 283 NHYSSNASSTSIHVHCSSSLCELANQCSSNKSSCPYQTHYLSQNSSSTGYLVQDILHMAT 342
           N Y+ NAS+TS  + CS   C  + +CSS  S CPYQ  Y S ++ + G L+QD+LH+AT
Sbjct: 152 NLYTPNASTTSSSIRCSDKRCFGSKKCSSPSSICPYQISY-SNSTGTKGTLLQDVLHLAT 211

Query: 343 DDSQLKPVDVKVTLGCGKVQAGKFSNVTAPNGLIGLGMGKLSIPSFLASQGLTPYSFSMC 402
           +D  L PV   VTLGCG+ Q G F    + NG++GLG+   S+PS LA   +T  SFSMC
Sbjct: 212 EDENLTPVKANVTLGCGQKQTGLFQRNNSVNGVLGLGIKGYSVPSLLAKANITANSFSMC 271

Query: 403 FGYY--GYGRTDFGDIGPVGQRQTPFNPA--SLSYNVTILQIIVTNRPRNVHFTAIIDSG 462
           FG      GR  FGD G   Q +TPF     S +Y V I  + V   P ++   A  D+G
Sbjct: 272 FGRVIGNVGRISFGDRGYTDQEETPFISVAPSTAYGVNISGVSVAGDPVDIRLFAKFDTG 331

Query: 463 ASFTYLTDPFYSIITENVS------------------------LATIFQQPNLNFTMEGG 522
           +SFT+L +P Y ++T++                           AT  Q P +  T  GG
Sbjct: 332 SSFTHLREPAYGVLTKSFDELVEDRRRPVDPELPFEFCYDLSPNATTIQFPLVEMTFIGG 391

Query: 523 RRFDFITSYVSVDTDDGPAL-CLVIVKST--DINVIGHNFLGGYRIVINREKMTLGWKEV 582
            +      + +  T +G  + CL ++KS    INVIG NF+ GYRIV +RE+M LGWK+ 
Sbjct: 392 SKIILNNPFFTARTQEGNVMYCLGVLKSVGLKINVIGQNFVAGYRIVFDRERMILGWKQS 451

Query: 583 DC---DSYDANT---------SSNDSPPPFRDSSPTTS-TPRKSNSTQPSPEIGTGDAMR 607
            C   +S ++ T         + + S PP R   PT S TP   N    +   GTG A  
Sbjct: 452 LCFEDESLESTTPPPPEVEAPAPSVSAPPPRSLPPTVSATPPPINPRNSTGNPGTGGAAN 511

BLAST of Chy2G028100 vs. TAIR 10
Match: AT3G51340.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 189.1 bits (479), Expect = 1.0e-47
Identity = 130/369 (35.23%), Postives = 183/369 (49.59%), Query Frame = 0

Query: 283 NHYSSNASSTSIHVHCSSSLCELANQCSSNKSSCPYQTHYLSQNSSSTGYLVQDILHMAT 342
           N Y+ NAS+TS  + CS   C  + +CSS +S CPYQ   LS N+ +TG L+QD+LH+ T
Sbjct: 153 NLYTPNASTTSSSIRCSDKRCFGSGKCSSPESICPYQI-ALSSNTVTTGTLLQDVLHLVT 212

Query: 343 DDSQLKPVDVKVTLGCGKVQAGKFSNVTAPNGLIGLGMGKLSIPSFLASQGLTPYSFSMC 402
           +D  LKPV+  VTLGCG+ Q G F    A NG++GL M + S+PS LA   +T  SFSMC
Sbjct: 213 EDEDLKPVNANVTLGCGQNQTGAFQTDIAVNGVLGLSMKEYSVPSLLAKANITANSFSMC 272

Query: 403 FG--YYGYGRTDFGDIGPVGQRQTPF--NPASLSYNVTILQIIVTNRPRNVHFTAIIDSG 462
           FG      GR  FGD G   Q +TP      S +Y V +  + V   P +V   A+ D+G
Sbjct: 273 FGRIISVVGRISFGDKGYTDQEETPLVSLETSTAYGVNVTGVSVGGVPVDVPLFALFDTG 332

Query: 463 ASFTYLTDPFYSIITE-----------------NVSLATIFQQPNLNFTMEGGR------ 522
           +SFT L +  Y + T+                         ++ +LN             
Sbjct: 333 SSFTLLLESAYGVFTKAFDDLMEDKRRPVDPDFPFEFCYDLREEHLNSDARPRHMQSKCY 392

Query: 523 ---RFDF-------ITSYVSVDTDDGPALCLVIVKSTDINVIGHNFLGGYRIVINREKMT 582
              R DF           VS   +     CL I+KS ++N+IG N + G+RIV +RE+M 
Sbjct: 393 NPCRDDFRWRIQNDSQESVSYSNEGTKMYCLGILKSINLNIIGQNLMSGHRIVFDRERMI 452

Query: 583 LGWKEVDCDSYDANTSSNDSPPPFRDSSPTTSTPRKSNSTQP--------SPEIGTGDAM 607
           LGWK+ +C   ++  S +  PP      P+ STP  + S  P        +   GTG A 
Sbjct: 453 LGWKQSNCFEDESLASESPPPPEIEAPPPSVSTPPPAASATPPTIDPRNSTRNSGTGGAA 512

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8VYV97.0e-6542.03Aspartyl protease family protein 1 OS=Arabidopsis thaliana OX=3702 GN=APF1 PE=2 ... [more]
Q9LX209.5e-3835.19Aspartic proteinase-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=At5g10080 ... [more]
Q9LEW31.5e-1129.89Aspartyl protease AED1 OS=Arabidopsis thaliana OX=3702 GN=AED1 PE=2 SV=1[more]
Q9M9A87.8e-0824.14Aspartyl protease APCB1 OS=Arabidopsis thaliana OX=3702 GN=APCB1 PE=1 SV=1[more]
A2ZC671.5e-0629.59Aspartic proteinase Asp1 OS=Oryza sativa subsp. indica OX=39946 GN=ASP1 PE=2 SV=... [more]
Match NameE-valueIdentityDescription
A0A0A0KGS09.5e-16685.51Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G50724... [more]
A0A5A7SLV51.6e-14475.92Aspartyl protease family protein 1-like OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A1S3B2V82.7e-14475.92aspartyl protease family protein 1-like OS=Cucumis melo OX=3656 GN=LOC103485160 ... [more]
W9SCT81.5e-11037.97Aspartic proteinase-like protein 1 OS=Morus notabilis OX=981085 GN=L484_026627 P... [more]
A0A371HV194.3e-11040.15Aspartyl protease family protein 1 (Fragment) OS=Mucuna pruriens OX=157652 GN=AP... [more]
Match NameE-valueIdentityDescription
XP_004135019.15.94e-20385.51aspartyl protease family protein 1 [Cucumis sativus] >KGN48950.1 hypothetical pr... [more]
KAA0025643.12.25e-17575.92aspartyl protease family protein 1-like [Cucumis melo var. makuwa] >TYK12516.1 a... [more]
XP_008440865.16.35e-17575.92PREDICTED: aspartyl protease family protein 1-like [Cucumis melo][more]
XP_038882976.11.21e-14365.08aspartyl protease family protein 1-like [Benincasa hispida][more]
KAG7034068.11.74e-14343.39Aspartyl protease family protein 1 [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
AT2G17760.14.9e-6642.03Eukaryotic aspartyl protease family protein [more]
AT4G35880.17.4e-6240.13Eukaryotic aspartyl protease family protein [more]
AT3G51330.13.9e-5538.86Eukaryotic aspartyl protease family protein [more]
AT3G51350.12.0e-5137.50Eukaryotic aspartyl protease family protein [more]
AT3G51340.11.0e-4735.23Eukaryotic aspartyl protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 83..221
e-value: 2.2E-17
score: 65.3
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 222..414
e-value: 2.2E-23
score: 85.3
coord: 1..76
e-value: 2.8E-14
score: 55.6
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 415..587
e-value: 6.6E-14
score: 53.4
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 279..555
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 7..220
IPR032861Xylanase inhibitor, N-terminalPFAMPF14543TAXi_Ncoord: 284..403
e-value: 6.2E-22
score: 78.7
coord: 8..76
e-value: 4.1E-9
score: 36.9
IPR032799Xylanase inhibitor, C-terminalPFAMPF14541TAXi_Ccoord: 95..218
e-value: 4.5E-6
score: 26.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 228..260
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 226..260
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 558..598
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 558..591
NoneNo IPR availablePANTHERPTHR13683:SF826ASPARTYL PROTEASE FAMILY PROTEIN 1coord: 278..477
NoneNo IPR availablePANTHERPTHR13683:SF826ASPARTYL PROTEASE FAMILY PROTEIN 1coord: 480..564
NoneNo IPR availablePANTHERPTHR13683:SF826ASPARTYL PROTEASE FAMILY PROTEIN 1coord: 1..229
IPR001461Aspartic peptidase A1 familyPANTHERPTHR13683ASPARTYL PROTEASEScoord: 480..564
coord: 1..229
IPR001461Aspartic peptidase A1 familyPANTHERPTHR13683ASPARTYL PROTEASEScoord: 278..477
IPR001969Aspartic peptidase, active sitePROSITEPS00141ASP_PROTEASEcoord: 114..125
IPR001969Aspartic peptidase, active sitePROSITEPS00141ASP_PROTEASEcoord: 453..464
IPR033121Peptidase family A1 domainPROSITEPS51767PEPTIDASE_A1coord: 257..549
score: 9.1063
IPR033121Peptidase family A1 domainPROSITEPS51767PEPTIDASE_A1coord: 1..218
score: 13.578876

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy2G028100.1Chy2G028100.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004190 aspartic-type endopeptidase activity