Chy2G027070 (gene) Cucumber (hystrix) v1

Overview


Name	Chy2G027070
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	alkaline ceramidase
Location	chrH02: 2778570 .. 2779485 (+)
RNA-Seq Expression	Chy2G027070
Synteny	Chy2G027070

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTGATGGAATATCAAGTTTCTGGGGTCCTGTAACATCTACGACAGAGTGCTGCGAGAGGAATTATGCTTATTCTTCTTATATTGCTGAGTTTTACAATACAGTCTCAAACATCCCAACCATTCTTTTGGCTCTGATCGGTCTTGCAAATGCCTTGCGACAGCGTTTCGAGAAGAGATTTAGTGTACTTCACATATCAAATATGATACTAGCCATTGGTAGCATGTTCTACCATGCCACATTGCAAAAAGTGTAAGTTGATATTTTGTTCAAACTACTTGAATAATTTAGGTTTTCTCGCTGCTGATTTATATTCATTTCATGTGGCCGGTGGAATGTTGGATATGTTTTTGAAATTTATGGTCTAATCTTGAATGATGAAACTTTGTTTGTTAACCAGGCAACAGCAAGGTGATGAAACACCAATGATCTGGGAGATGCTTCTTTACATGTACATTCTCTATTCACCTGATTGGCATTACCGAAGTACGATGCCAACGTTTCTCTTCCTCTATGGAGCCATGTTCGCTGTGGCTCACTCGATTCTTCGTTATGACATTGGTTTCAAGGTGCACTATGTGATTCTCTGTTTCCTCTGCATACCTCGGATGTACAAATATTATATATACACAGAAGATGCCTCGGCTAAGCGGTTAGCAAGGCTGTACTCATTGACTCTTCTCTTAGGCACTTTCTGTTGGGTTAGCGATCGGGGTTTGTGCGAGAAGATATCACATTGGATCATCAATCCTCAAGGTCATGCCCTGTGGCATGTTTTCATGGGTTTGAGTTCCTATTATGCGAACACATTCTTAATGTTTTGCCGGGCGCAACAAAGAGGATGGTCCCCTAAAGTGCTTCATTTAATGGGAGTTCTCCCCTACGTGAAGATTGAGAAACCAAAAACACAGTAA

mRNA sequence

ATGGGTGATGGAATATCAAGTTTCTGGGGTCCTGTAACATCTACGACAGAGTGCTGCGAGAGGAATTATGCTTATTCTTCTTATATTGCTGAGTTTTACAATACAGTCTCAAACATCCCAACCATTCTTTTGGCTCTGATCGGTCTTGCAAATGCCTTGCGACAGCGTTTCGAGAAGAGATTTAGTGTACTTCACATATCAAATATGATACTAGCCATTGGTAGCATGTTCTACCATGCCACATTGCAAAAAGTGCAACAGCAAGGTGATGAAACACCAATGATCTGGGAGATGCTTCTTTACATGTACATTCTCTATTCACCTGATTGGCATTACCGAAGTACGATGCCAACGTTTCTCTTCCTCTATGGAGCCATGTTCGCTGTGGCTCACTCGATTCTTCGTTATGACATTGGTTTCAAGGTGCACTATGTGATTCTCTGTTTCCTCTGCATACCTCGGATGTACAAATATTATATATACACAGAAGATGCCTCGGCTAAGCGGTTAGCAAGGCTGTACTCATTGACTCTTCTCTTAGGCACTTTCTGTTGGGTTAGCGATCGGGGTTTGTGCGAGAAGATATCACATTGGATCATCAATCCTCAAGGTCATGCCCTGTGGCATGTTTTCATGGGTTTGAGTTCCTATTATGCGAACACATTCTTAATGTTTTGCCGGGCGCAACAAAGAGGATGGTCCCCTAAAGTGCTTCATTTAATGGGAGTTCTCCCCTACGTGAAGATTGAGAAACCAAAAACACAGTAA

Coding sequence (CDS)

ATGGGTGATGGAATATCAAGTTTCTGGGGTCCTGTAACATCTACGACAGAGTGCTGCGAGAGGAATTATGCTTATTCTTCTTATATTGCTGAGTTTTACAATACAGTCTCAAACATCCCAACCATTCTTTTGGCTCTGATCGGTCTTGCAAATGCCTTGCGACAGCGTTTCGAGAAGAGATTTAGTGTACTTCACATATCAAATATGATACTAGCCATTGGTAGCATGTTCTACCATGCCACATTGCAAAAAGTGCAACAGCAAGGTGATGAAACACCAATGATCTGGGAGATGCTTCTTTACATGTACATTCTCTATTCACCTGATTGGCATTACCGAAGTACGATGCCAACGTTTCTCTTCCTCTATGGAGCCATGTTCGCTGTGGCTCACTCGATTCTTCGTTATGACATTGGTTTCAAGGTGCACTATGTGATTCTCTGTTTCCTCTGCATACCTCGGATGTACAAATATTATATATACACAGAAGATGCCTCGGCTAAGCGGTTAGCAAGGCTGTACTCATTGACTCTTCTCTTAGGCACTTTCTGTTGGGTTAGCGATCGGGGTTTGTGCGAGAAGATATCACATTGGATCATCAATCCTCAAGGTCATGCCCTGTGGCATGTTTTCATGGGTTTGAGTTCCTATTATGCGAACACATTCTTAATGTTTTGCCGGGCGCAACAAAGAGGATGGTCCCCTAAAGTGCTTCATTTAATGGGAGTTCTCCCCTACGTGAAGATTGAGAAACCAAAAACACAGTAA

Protein sequence

MGDGISSFWGPVTSTTECCERNYAYSSYIAEFYNTVSNIPTILLALIGLANALRQRFEKRFSVLHISNMILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFLFLYGAMFAVAHSILRYDIGFKVHYVILCFLCIPRMYKYYIYTEDASAKRLARLYSLTLLLGTFCWVSDRGLCEKISHWIINPQGHALWHVFMGLSSYYANTFLMFCRAQQRGWSPKVLHLMGVLPYVKIEKPKTQ*

Homology

[Close]

BLAST of Chy2G027070 vs. ExPASy Swiss-Prot
Match: Q94IB9 (Alkaline ceramidase OS=Arabidopsis thaliana OX=3702 GN=ACER PE=2 SV=1)

HSP 1 Score: 444.1 bits (1141), Expect = 1.1e-123
Identity = 204/255 (80.00%), Postives = 225/255 (88.24%), Query Frame = 0


Query: 1   MGDGISSFWGPVTSTTECCERNYAYSSYIAEFYNTVSNIPTILLALIGLANALRQRFEKR 60
           M DGISSFWGPVTST ECCE NYAYSSYIAEFYNT+SN+P ILLALIGL NALRQRFEKR
Sbjct: 1   MADGISSFWGPVTSTIECCEMNYAYSSYIAEFYNTISNVPGILLALIGLVNALRQRFEKR 60

Query: 61  FSVLHISNMILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL 120
           FS+LHISNMILAIGSM YHATLQ VQQQ DETPM+WE+LLYMYILYSPDWHYRSTMPTFL
Sbjct: 61  FSILHISNMILAIGSMLYHATLQHVQQQSDETPMVWEILLYMYILYSPDWHYRSTMPTFL 120

Query: 121 FLYGAMFAVAHSILRYDIGFKVHYVILCFLCIPRMYKYYIYTEDASAKRLARLYSLTLLL 180
           FLYGA FA+ H+ LR+ IGFKVHYVILC LCIPRMYKYYI+TED +AKR+A+ Y  T+L+
Sbjct: 121 FLYGAAFAIVHAYLRFGIGFKVHYVILCLLCIPRMYKYYIHTEDTAAKRIAKWYVATILV 180

Query: 181 GTFCWVSDRGLCEKISHWIINPQGHALWHVFMGLSSYYANTFLMFCRAQQRGWSPKVLHL 240
           G+ CW  DR  C+ IS W +NPQGHALWHVFM  +SY ANTFLMFCRAQQRGW+PKV + 
Sbjct: 181 GSICWFCDRVFCKTISQWPVNPQGHALWHVFMSFNSYCANTFLMFCRAQQRGWNPKVKYF 240

Query: 241 MGVLPYVKIEKPKTQ 256
           +GVLPYVKIEKPKTQ
Sbjct: 241 LGVLPYVKIEKPKTQ 255

[Close]

BLAST of Chy2G027070 vs. ExPASy Swiss-Prot
Match: Q9D099 (Alkaline ceramidase 3 OS=Mus musculus OX=10090 GN=Acer3 PE=1 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 6.4e-28
Identity = 82/259 (31.66%), Postives = 126/259 (48.65%), Query Frame = 0


Query: 8   FWGPVTSTTECCERNYAYSSYIAEFYNTVSNIPTILLALIGLANALRQRFEKRFSVLHIS 67
           +WGP TST + CE NY  + ++AEF+NTVSN+  I+  + G    +R R EKR+   +++
Sbjct: 10  YWGPTTSTLDWCEENYVVTLFVAEFWNTVSNLIMIIPPIFGAIQGIRDRLEKRYIAAYLA 69

Query: 68  NMILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILY-------SPDWHYRSTMPTFL 127
             ++ +GS  +H TL+   Q  DE PMI+   +++Y ++       S ++H   T    L
Sbjct: 70  LTVVGMGSWCFHMTLKYEMQLLDELPMIYSCCIFVYCMFECFKTKSSINYHLLFT----L 129

Query: 128 FLYGAMFAVAHSILRYDIGFKVHYVILCFLCIPRMYKYYIYTEDASAKRLARLYSLTL-L 187
           FLY       +  ++  I  +V Y +L F  + R    YI T      R     SLT+ L
Sbjct: 130 FLYSLTVTTIYLKVKEPIFHQVMYGMLVFTLVLR--SIYIVTWVYPWLRGLGYTSLTVFL 189

Query: 188 LGTFCWVSDRGLCEKISHWI--------INPQGHALWHVFMGLSSYYANTFLMFCRAQQR 247
           LG   W  D   C+ + ++         +  Q HA WH+  GL SY    F ++ R    
Sbjct: 190 LGFLLWNIDNIFCDSLRNFRKRVPPVLGVTTQFHAWWHILTGLGSYLHILFSLYTRTLYL 249

Query: 248 GWSPKVLHLMGVLPYVKIE 251
            + PKV  L G+ P V  E
Sbjct: 250 RYRPKVKFLFGIWPAVMFE 262

[Close]

BLAST of Chy2G027070 vs. ExPASy Swiss-Prot
Match: Q9NUN7 (Alkaline ceramidase 3 OS=Homo sapiens OX=9606 GN=ACER3 PE=1 SV=3)

HSP 1 Score: 120.9 bits (302), Expect = 2.0e-26
Identity = 81/259 (31.27%), Postives = 126/259 (48.65%), Query Frame = 0


Query: 8   FWGPVTSTTECCERNYAYSSYIAEFYNTVSNIPTILLALIGLANALRQRFEKRFSVLHIS 67
           +WGP TST + CE NY+ + YIAEF+NTVSN+  I+  + G   ++R   EKR+   +++
Sbjct: 10  YWGPTTSTLDWCEENYSVTWYIAEFWNTVSNLIMIIPPMFGAVQSVRDGLEKRYIASYLA 69

Query: 68  NMILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILY-------SPDWHYRSTMPTFL 127
             ++ +GS  +H TL+   Q  DE PMI+   +++Y ++       S ++H   T    L
Sbjct: 70  LTVVGMGSWCFHMTLKYEMQLLDELPMIYSCCIFVYCMFECFKIKNSVNYHLLFT----L 129

Query: 128 FLYGAMFAVAHSILRYDIGFKVHYVILCFLCIPRMYKYYIYTEDASAKRLARLYSLTL-L 187
            L+  +    +  ++  I  +V Y +L F  + R    YI T      R     SL + L
Sbjct: 130 VLFSLIVTTVYLKVKEPIFHQVMYGMLVFTLVLR--SIYIVTWVYPWLRGLGYTSLGIFL 189

Query: 188 LGTFCWVSDRGLCEKISHWI--------INPQGHALWHVFMGLSSYYANTFLMFCRAQQR 247
           LG   W  D   CE + ++         I  Q HA WH+  GL SY    F ++ R    
Sbjct: 190 LGFLFWNIDNIFCESLRNFRKKVPPIIGITTQFHAWWHILTGLGSYLHILFSLYTRTLYL 249

Query: 248 GWSPKVLHLMGVLPYVKIE 251
            + PKV  L G+ P +  E
Sbjct: 250 RYRPKVKFLFGIWPVILFE 262

[Close]

BLAST of Chy2G027070 vs. ExPASy Swiss-Prot
Match: Q6TMJ1 (Putative alkaline ceramidase dcd3A OS=Dictyostelium discoideum OX=44689 GN=dcd3A PE=2 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 1.3e-17
Identity = 74/273 (27.11%), Postives = 120/273 (43.96%), Query Frame = 0


Query: 9   WGPVTSTTECCERNYAYSSYIAEFYNTVSNIPTILLALIGL---------------ANAL 68
           +G  T++ + CE NY YS YIAEFYNT S++   L  + G+                  L
Sbjct: 10  YGTPTASIDWCELNYTYSPYIAEFYNTFSSLIISLFGIYGIWIMMPNFGTGVEKEHIKIL 69

Query: 69  RQRFEKRFSVL-HISNMILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWH- 128
           +Q   +   +L +IS +++ +GS FYHATL    Q  DE PMI+  L+ +YI+ +     
Sbjct: 70  KQLDVRNKVILSYISLIVVGVGSAFYHATLLYQNQLFDELPMIYTALIMLYIMVTVGEEK 129

Query: 129 --------------YRSTMPTFLFLYGAMFAVA-------HSILRYDIGFKVHYVILCFL 188
                          R  +P  L  YG    +          IL+   G  V YV+   +
Sbjct: 130 TKKGFKGGVLGNSLLRHLLPYLLIAYGLFVTITILVIQDQPKILQVSFGALVFYVVFHSI 189

Query: 189 CIPRMYKYYIYTEDASAKRLARLYSLTLLLGTFCWVSDRGLCEKISHWIINPQGHALWHV 244
            +    K      +  +  L +   +++L+G  CWV +R  C+  +      Q HA WH 
Sbjct: 190 YLINKKKPDGMPSNPDS-YLYKYAFVSMLVGFTCWVVERYFCK--NGKTFGFQLHAFWHF 249

[Close]

BLAST of Chy2G027070 vs. ExPASy Swiss-Prot
Match: Q9VIP7 (Alkaline ceramidase OS=Drosophila melanogaster OX=7227 GN=bwa PE=2 SV=2)

HSP 1 Score: 85.9 bits (211), Expect = 7.3e-16
Identity = 67/216 (31.02%), Postives = 109/216 (50.46%), Query Frame = 0


Query: 11  PVTSTTECCERNYAYSSYIAEFYNTVSNIPTILL--ALIGLANALRQRFEKRFSVLHISN 70
           P +S  + CE NY  SS IAEF NT SN   ILL   LI L     +       V+ +  
Sbjct: 22  PGSSPVDWCEGNYLISSNIAEFVNTFSNFLFILLPPVLIMLFKEYGRFVTPGIHVIWVLL 81

Query: 71  MILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHY----RSTMPTFLFLYG 130
           +++ + SM++HATL  + Q  DE  ++W + +  + L+ P  +Y    ++   TF +L  
Sbjct: 82  IVVGLSSMYFHATLSLIGQLLDELAILW-VFMAAFSLFYPKRYYPKFVKNDRKTFSWLM- 141

Query: 131 AMFAVAHSILRYDIGFKVHYVILCFLCIPRMYKYYIYTEDASAKRLARL---YSLTLLLG 190
            + A+A + L +     V+  +L F+ +P M   Y   +  S +R+ RL    +    + 
Sbjct: 142 LLSAIAATGLSWWKPI-VNAFVLMFMSVPTMVMLYTELQRVSDQRVYRLGIRSTTVWAVA 201

Query: 191 TFCWVSDRGLCEKISHWIINPQGHALWHVFMGLSSY 218
            FCW++DR  CE  S  I  P  H  WH+F+ +++Y
Sbjct: 202 VFCWINDRIFCEAWSS-INFPYLHGFWHIFIFIAAY 233

[Close]

BLAST of Chy2G027070 vs. ExPASy TrEMBL
Match: A0A1S3B0Z4 (alkaline ceramidase 3 OS=Cucumis melo OX=3656 GN=LOC103484652 PE=3 SV=1)

HSP 1 Score: 529.3 bits (1362), Expect = 9.2e-147
Identity = 253/255 (99.22%), Postives = 253/255 (99.22%), Query Frame = 0


Query: 1   MGDGISSFWGPVTSTTECCERNYAYSSYIAEFYNTVSNIPTILLALIGLANALRQRFEKR 60
           M DGISSFWGPVTSTTECCERNYAYSSYIAEFYNTVSNIPTILLALIGLANALRQRFEKR
Sbjct: 1   MADGISSFWGPVTSTTECCERNYAYSSYIAEFYNTVSNIPTILLALIGLANALRQRFEKR 60

Query: 61  FSVLHISNMILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL 120
           FSVLHISNMILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL
Sbjct: 61  FSVLHISNMILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL 120

Query: 121 FLYGAMFAVAHSILRYDIGFKVHYVILCFLCIPRMYKYYIYTEDASAKRLARLYSLTLLL 180
           FLYGAMFAVAHSILRYDIGFKVHYVILC LCIPRMYKYYIYTEDASAKRLARLYSLTLLL
Sbjct: 121 FLYGAMFAVAHSILRYDIGFKVHYVILCLLCIPRMYKYYIYTEDASAKRLARLYSLTLLL 180

Query: 181 GTFCWVSDRGLCEKISHWIINPQGHALWHVFMGLSSYYANTFLMFCRAQQRGWSPKVLHL 240
           GTFCWVSDRGLCEKISHWIINPQGHALWHVFMGLSSYYANTFLMFCRAQQRGWSPKVLHL
Sbjct: 181 GTFCWVSDRGLCEKISHWIINPQGHALWHVFMGLSSYYANTFLMFCRAQQRGWSPKVLHL 240

Query: 241 MGVLPYVKIEKPKTQ 256
           MGVLPYVKIEKPKTQ
Sbjct: 241 MGVLPYVKIEKPKTQ 255

[Close]

BLAST of Chy2G027070 vs. ExPASy TrEMBL
Match: A0A6J1KSV2 (alkaline ceramidase OS=Cucurbita maxima OX=3661 GN=LOC111497288 PE=3 SV=1)

HSP 1 Score: 501.9 bits (1291), Expect = 1.6e-138
Identity = 236/255 (92.55%), Postives = 245/255 (96.08%), Query Frame = 0


Query: 1   MGDGISSFWGPVTSTTECCERNYAYSSYIAEFYNTVSNIPTILLALIGLANALRQRFEKR 60
           M DGISSFWGPVTST ECCE+NYA+SSYIAEFYNT+SNIPT+LLALIGLANALRQ FEKR
Sbjct: 1   MADGISSFWGPVTSTIECCEKNYAFSSYIAEFYNTISNIPTVLLALIGLANALRQGFEKR 60

Query: 61  FSVLHISNMILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL 120
           FSVLHISNMILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL
Sbjct: 61  FSVLHISNMILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL 120

Query: 121 FLYGAMFAVAHSILRYDIGFKVHYVILCFLCIPRMYKYYIYTEDASAKRLARLYSLTLLL 180
           FLYGA+FAVAHSILRYDIGFK+HYVILC LCIPRMYKYYIYTEDASAKRLARLY LTLLL
Sbjct: 121 FLYGAIFAVAHSILRYDIGFKLHYVILCLLCIPRMYKYYIYTEDASAKRLARLYLLTLLL 180

Query: 181 GTFCWVSDRGLCEKISHWIINPQGHALWHVFMGLSSYYANTFLMFCRAQQRGWSPKVLHL 240
           G FCWV DRG CEKISHW INPQGHALWH FMGLSSY+ANTFLMFCRAQQRGW+PKVLHL
Sbjct: 181 GIFCWVIDRGFCEKISHWTINPQGHALWHGFMGLSSYFANTFLMFCRAQQRGWAPKVLHL 240

Query: 241 MGVLPYVKIEKPKTQ 256
           MG+LPYVKIEKPKTQ
Sbjct: 241 MGILPYVKIEKPKTQ 255

[Close]

BLAST of Chy2G027070 vs. ExPASy TrEMBL
Match: A0A6J1EME1 (alkaline ceramidase OS=Cucurbita moschata OX=3662 GN=LOC111433883 PE=3 SV=1)

HSP 1 Score: 501.1 bits (1289), Expect = 2.7e-138
Identity = 237/255 (92.94%), Postives = 245/255 (96.08%), Query Frame = 0


Query: 1   MGDGISSFWGPVTSTTECCERNYAYSSYIAEFYNTVSNIPTILLALIGLANALRQRFEKR 60
           M DGISSFWGPVTST ECCE+NYAYSSYIAEFYNT+SNIPT+LLALIGLANALRQ FEKR
Sbjct: 1   MADGISSFWGPVTSTIECCEKNYAYSSYIAEFYNTISNIPTVLLALIGLANALRQGFEKR 60

Query: 61  FSVLHISNMILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL 120
           FSVLHISNMILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL
Sbjct: 61  FSVLHISNMILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL 120

Query: 121 FLYGAMFAVAHSILRYDIGFKVHYVILCFLCIPRMYKYYIYTEDASAKRLARLYSLTLLL 180
           FLYGA+FAVAHSILRYDIGFK+HYVILC LCIPRMYKYYIYTEDASAKRLARLY LTLLL
Sbjct: 121 FLYGAIFAVAHSILRYDIGFKLHYVILCLLCIPRMYKYYIYTEDASAKRLARLYLLTLLL 180

Query: 181 GTFCWVSDRGLCEKISHWIINPQGHALWHVFMGLSSYYANTFLMFCRAQQRGWSPKVLHL 240
           G FCWV DR LCEKISHW INPQGHALWH FMGLSSY+ANTFLMFCRAQQRGW+PKVLHL
Sbjct: 181 GIFCWVIDRCLCEKISHWSINPQGHALWHGFMGLSSYFANTFLMFCRAQQRGWAPKVLHL 240

Query: 241 MGVLPYVKIEKPKTQ 256
           MG+LPYVKIEKPKTQ
Sbjct: 241 MGILPYVKIEKPKTQ 255

[Close]

BLAST of Chy2G027070 vs. ExPASy TrEMBL
Match: A0A6J1BUA9 (alkaline ceramidase OS=Momordica charantia OX=3673 GN=LOC111005428 PE=3 SV=1)

HSP 1 Score: 496.5 bits (1277), Expect = 6.6e-137
Identity = 231/255 (90.59%), Postives = 244/255 (95.69%), Query Frame = 0


Query: 1   MGDGISSFWGPVTSTTECCERNYAYSSYIAEFYNTVSNIPTILLALIGLANALRQRFEKR 60
           M DGISSFWGPVTST ECCE+NYAYS+Y+AEF+NT+SNIPTILLALIGL+NAL+QRFEKR
Sbjct: 1   MADGISSFWGPVTSTMECCEKNYAYSTYVAEFFNTISNIPTILLALIGLSNALKQRFEKR 60

Query: 61  FSVLHISNMILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL 120
           FSVLHISNMILAIGSMFYHATLQK+QQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL
Sbjct: 61  FSVLHISNMILAIGSMFYHATLQKLQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL 120

Query: 121 FLYGAMFAVAHSILRYDIGFKVHYVILCFLCIPRMYKYYIYTEDASAKRLARLYSLTLLL 180
           FLYGA+FAVAHSILRY IGFKVHYVILC LCIPRMYKYYIYTEDASAKRLARLY LTL L
Sbjct: 121 FLYGAIFAVAHSILRYGIGFKVHYVILCLLCIPRMYKYYIYTEDASAKRLARLYLLTLFL 180

Query: 181 GTFCWVSDRGLCEKISHWIINPQGHALWHVFMGLSSYYANTFLMFCRAQQRGWSPKVLHL 240
           G  CWV DRG CEKISHW+INPQGHALWHVFMGL SY+ANTFLMFCRAQQRGW+PKVLHL
Sbjct: 181 GILCWVIDRGFCEKISHWMINPQGHALWHVFMGLGSYFANTFLMFCRAQQRGWAPKVLHL 240

Query: 241 MGVLPYVKIEKPKTQ 256
           MG+LPYVKIEKPKTQ
Sbjct: 241 MGILPYVKIEKPKTQ 255

[Close]

BLAST of Chy2G027070 vs. ExPASy TrEMBL
Match: A0A5D3CM37 (Alkaline ceramidase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G006050 PE=3 SV=1)

HSP 1 Score: 491.1 bits (1263), Expect = 2.8e-135
Identity = 243/286 (84.97%), Postives = 248/286 (86.71%), Query Frame = 0


Query: 1   MGDGISSFWGPVTSTTECCERNYAYSSYIAEFYNTVSNIPTILLALIGLANALRQRFEKR 60
           M DGISSFWGPVTSTTECCERNYAYSSYIAEFYNTVSNIPTILLALIGLANALRQRFEKR
Sbjct: 1   MADGISSFWGPVTSTTECCERNYAYSSYIAEFYNTVSNIPTILLALIGLANALRQRFEKR 60

Query: 61  FSVLHISNMILAIG--------------------------SMFYHATLQKV-----QQQG 120
           FSVLHISNMILAIG                          +++    + K+     QQQG
Sbjct: 61  FSVLHISNMILAIGRGLAADLYSFHVAGGMLDMFLKFMVLNVYLQQAMMKLCFLTRQQQG 120

Query: 121 DETPMIWEMLLYMYILYSPDWHYRSTMPTFLFLYGAMFAVAHSILRYDIGFKVHYVILCF 180
           DETPMIWEMLLYMYILYSPDWHYRSTMPTFLFLYGAMFAVAHSILRYDIGFKVHYVILC 
Sbjct: 121 DETPMIWEMLLYMYILYSPDWHYRSTMPTFLFLYGAMFAVAHSILRYDIGFKVHYVILCL 180

Query: 181 LCIPRMYKYYIYTEDASAKRLARLYSLTLLLGTFCWVSDRGLCEKISHWIINPQGHALWH 240
           LCIPRMYKYYIYTEDASAKRLARLYSLTLLLGTFCWVSDRGLCEKISHWIINPQGHALWH
Sbjct: 181 LCIPRMYKYYIYTEDASAKRLARLYSLTLLLGTFCWVSDRGLCEKISHWIINPQGHALWH 240

Query: 241 VFMGLSSYYANTFLMFCRAQQRGWSPKVLHLMGVLPYVKIEKPKTQ 256
           VFMGLSSYYANTFLMFCRAQQRGWSPKVLHLMGVLPYVKIEKPKTQ
Sbjct: 241 VFMGLSSYYANTFLMFCRAQQRGWSPKVLHLMGVLPYVKIEKPKTQ 286

[Close]

BLAST of Chy2G027070 vs. NCBI nr
Match: XP_008440061.1 (PREDICTED: alkaline ceramidase 3 [Cucumis melo] >XP_008440062.1 PREDICTED: alkaline ceramidase 3 [Cucumis melo])

HSP 1 Score: 528 bits (1361), Expect = 1.56e-189
Identity = 253/255 (99.22%), Postives = 253/255 (99.22%), Query Frame = 0


Query: 1   MGDGISSFWGPVTSTTECCERNYAYSSYIAEFYNTVSNIPTILLALIGLANALRQRFEKR 60
           M DGISSFWGPVTSTTECCERNYAYSSYIAEFYNTVSNIPTILLALIGLANALRQRFEKR
Sbjct: 1   MADGISSFWGPVTSTTECCERNYAYSSYIAEFYNTVSNIPTILLALIGLANALRQRFEKR 60

Query: 61  FSVLHISNMILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL 120
           FSVLHISNMILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL
Sbjct: 61  FSVLHISNMILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL 120

Query: 121 FLYGAMFAVAHSILRYDIGFKVHYVILCFLCIPRMYKYYIYTEDASAKRLARLYSLTLLL 180
           FLYGAMFAVAHSILRYDIGFKVHYVILC LCIPRMYKYYIYTEDASAKRLARLYSLTLLL
Sbjct: 121 FLYGAMFAVAHSILRYDIGFKVHYVILCLLCIPRMYKYYIYTEDASAKRLARLYSLTLLL 180

Query: 181 GTFCWVSDRGLCEKISHWIINPQGHALWHVFMGLSSYYANTFLMFCRAQQRGWSPKVLHL 240
           GTFCWVSDRGLCEKISHWIINPQGHALWHVFMGLSSYYANTFLMFCRAQQRGWSPKVLHL
Sbjct: 181 GTFCWVSDRGLCEKISHWIINPQGHALWHVFMGLSSYYANTFLMFCRAQQRGWSPKVLHL 240

Query: 241 MGVLPYVKIEKPKTQ 255
           MGVLPYVKIEKPKTQ
Sbjct: 241 MGVLPYVKIEKPKTQ 255

[Close]

BLAST of Chy2G027070 vs. NCBI nr
Match: XP_004134791.1 (alkaline ceramidase [Cucumis sativus] >XP_031742854.1 alkaline ceramidase [Cucumis sativus] >KAE8647646.1 hypothetical protein Csa_003199 [Cucumis sativus])

HSP 1 Score: 526 bits (1355), Expect = 1.28e-188
Identity = 252/255 (98.82%), Postives = 252/255 (98.82%), Query Frame = 0


Query: 1   MGDGISSFWGPVTSTTECCERNYAYSSYIAEFYNTVSNIPTILLALIGLANALRQRFEKR 60
           M DGISSFWGPVTSTTECCERNYAYSSYIAEFYNTVSNIPTILLALIGLANALRQRFEKR
Sbjct: 1   MADGISSFWGPVTSTTECCERNYAYSSYIAEFYNTVSNIPTILLALIGLANALRQRFEKR 60

Query: 61  FSVLHISNMILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL 120
           FSVLHISNMILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL
Sbjct: 61  FSVLHISNMILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL 120

Query: 121 FLYGAMFAVAHSILRYDIGFKVHYVILCFLCIPRMYKYYIYTEDASAKRLARLYSLTLLL 180
           FLYGAMFAVAHSILRYDIGFKVHYVILC LCIPRMYKYYIYTEDASAKRLARLYSLTLLL
Sbjct: 121 FLYGAMFAVAHSILRYDIGFKVHYVILCLLCIPRMYKYYIYTEDASAKRLARLYSLTLLL 180

Query: 181 GTFCWVSDRGLCEKISHWIINPQGHALWHVFMGLSSYYANTFLMFCRAQQRGWSPKVLHL 240
           GTFCWVSDRGLCE ISHWIINPQGHALWHVFMGLSSYYANTFLMFCRAQQRGWSPKVLHL
Sbjct: 181 GTFCWVSDRGLCETISHWIINPQGHALWHVFMGLSSYYANTFLMFCRAQQRGWSPKVLHL 240

Query: 241 MGVLPYVKIEKPKTQ 255
           MGVLPYVKIEKPKTQ
Sbjct: 241 MGVLPYVKIEKPKTQ 255

[Close]

BLAST of Chy2G027070 vs. NCBI nr
Match: XP_038882449.1 (alkaline ceramidase [Benincasa hispida] >XP_038882450.1 alkaline ceramidase [Benincasa hispida])

HSP 1 Score: 509 bits (1310), Expect = 9.33e-182
Identity = 242/255 (94.90%), Postives = 247/255 (96.86%), Query Frame = 0


Query: 1   MGDGISSFWGPVTSTTECCERNYAYSSYIAEFYNTVSNIPTILLALIGLANALRQRFEKR 60
           M DGISSFWGPVTSTTECCE+NYA SSYIAEFYNTVSNIPTILLALIGL NALRQRFEKR
Sbjct: 1   MADGISSFWGPVTSTTECCEKNYASSSYIAEFYNTVSNIPTILLALIGLTNALRQRFEKR 60

Query: 61  FSVLHISNMILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL 120
           FSVLHISNMILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL
Sbjct: 61  FSVLHISNMILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL 120

Query: 121 FLYGAMFAVAHSILRYDIGFKVHYVILCFLCIPRMYKYYIYTEDASAKRLARLYSLTLLL 180
           FLYGAMFAVAHSILRYDIGFKVHYVILC LC+PRMYKYYIYT+DASAKRLARLYSLTLLL
Sbjct: 121 FLYGAMFAVAHSILRYDIGFKVHYVILCLLCMPRMYKYYIYTKDASAKRLARLYSLTLLL 180

Query: 181 GTFCWVSDRGLCEKISHWIINPQGHALWHVFMGLSSYYANTFLMFCRAQQRGWSPKVLHL 240
           G FCWV D+G CEKISHWIINPQGHALWHV MGLSSYYANTFLMFCRAQQRGW+PKVLHL
Sbjct: 181 GIFCWVMDQGFCEKISHWIINPQGHALWHVIMGLSSYYANTFLMFCRAQQRGWAPKVLHL 240

Query: 241 MGVLPYVKIEKPKTQ 255
           MGVLPYVKIEKPKTQ
Sbjct: 241 MGVLPYVKIEKPKTQ 255

[Close]

BLAST of Chy2G027070 vs. NCBI nr
Match: XP_023003815.1 (alkaline ceramidase [Cucurbita maxima] >XP_023003816.1 alkaline ceramidase [Cucurbita maxima] >XP_023003817.1 alkaline ceramidase [Cucurbita maxima] >XP_023003819.1 alkaline ceramidase [Cucurbita maxima] >XP_023003820.1 alkaline ceramidase [Cucurbita maxima])

HSP 1 Score: 501 bits (1290), Expect = 1.05e-178
Identity = 236/255 (92.55%), Postives = 245/255 (96.08%), Query Frame = 0


Query: 1   MGDGISSFWGPVTSTTECCERNYAYSSYIAEFYNTVSNIPTILLALIGLANALRQRFEKR 60
           M DGISSFWGPVTST ECCE+NYA+SSYIAEFYNT+SNIPT+LLALIGLANALRQ FEKR
Sbjct: 1   MADGISSFWGPVTSTIECCEKNYAFSSYIAEFYNTISNIPTVLLALIGLANALRQGFEKR 60

Query: 61  FSVLHISNMILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL 120
           FSVLHISNMILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL
Sbjct: 61  FSVLHISNMILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL 120

Query: 121 FLYGAMFAVAHSILRYDIGFKVHYVILCFLCIPRMYKYYIYTEDASAKRLARLYSLTLLL 180
           FLYGA+FAVAHSILRYDIGFK+HYVILC LCIPRMYKYYIYTEDASAKRLARLY LTLLL
Sbjct: 121 FLYGAIFAVAHSILRYDIGFKLHYVILCLLCIPRMYKYYIYTEDASAKRLARLYLLTLLL 180

Query: 181 GTFCWVSDRGLCEKISHWIINPQGHALWHVFMGLSSYYANTFLMFCRAQQRGWSPKVLHL 240
           G FCWV DRG CEKISHW INPQGHALWH FMGLSSY+ANTFLMFCRAQQRGW+PKVLHL
Sbjct: 181 GIFCWVIDRGFCEKISHWTINPQGHALWHGFMGLSSYFANTFLMFCRAQQRGWAPKVLHL 240

Query: 241 MGVLPYVKIEKPKTQ 255
           MG+LPYVKIEKPKTQ
Sbjct: 241 MGILPYVKIEKPKTQ 255

[Close]

BLAST of Chy2G027070 vs. NCBI nr
Match: XP_022926905.1 (alkaline ceramidase [Cucurbita moschata] >XP_022926906.1 alkaline ceramidase [Cucurbita moschata] >XP_022926907.1 alkaline ceramidase [Cucurbita moschata] >XP_022926908.1 alkaline ceramidase [Cucurbita moschata])

HSP 1 Score: 500 bits (1288), Expect = 2.11e-178
Identity = 237/255 (92.94%), Postives = 245/255 (96.08%), Query Frame = 0


Query: 1   MGDGISSFWGPVTSTTECCERNYAYSSYIAEFYNTVSNIPTILLALIGLANALRQRFEKR 60
           M DGISSFWGPVTST ECCE+NYAYSSYIAEFYNT+SNIPT+LLALIGLANALRQ FEKR
Sbjct: 1   MADGISSFWGPVTSTIECCEKNYAYSSYIAEFYNTISNIPTVLLALIGLANALRQGFEKR 60

Query: 61  FSVLHISNMILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL 120
           FSVLHISNMILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL
Sbjct: 61  FSVLHISNMILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL 120

Query: 121 FLYGAMFAVAHSILRYDIGFKVHYVILCFLCIPRMYKYYIYTEDASAKRLARLYSLTLLL 180
           FLYGA+FAVAHSILRYDIGFK+HYVILC LCIPRMYKYYIYTEDASAKRLARLY LTLLL
Sbjct: 121 FLYGAIFAVAHSILRYDIGFKLHYVILCLLCIPRMYKYYIYTEDASAKRLARLYLLTLLL 180

Query: 181 GTFCWVSDRGLCEKISHWIINPQGHALWHVFMGLSSYYANTFLMFCRAQQRGWSPKVLHL 240
           G FCWV DR LCEKISHW INPQGHALWH FMGLSSY+ANTFLMFCRAQQRGW+PKVLHL
Sbjct: 181 GIFCWVIDRCLCEKISHWSINPQGHALWHGFMGLSSYFANTFLMFCRAQQRGWAPKVLHL 240

Query: 241 MGVLPYVKIEKPKTQ 255
           MG+LPYVKIEKPKTQ
Sbjct: 241 MGILPYVKIEKPKTQ 255

[Close]

BLAST of Chy2G027070 vs. TAIR 10
Match: AT4G22330.1 (Alkaline phytoceramidase (aPHC) )

HSP 1 Score: 444.1 bits (1141), Expect = 7.5e-125
Identity = 204/255 (80.00%), Postives = 225/255 (88.24%), Query Frame = 0


Query: 1   MGDGISSFWGPVTSTTECCERNYAYSSYIAEFYNTVSNIPTILLALIGLANALRQRFEKR 60
           M DGISSFWGPVTST ECCE NYAYSSYIAEFYNT+SN+P ILLALIGL NALRQRFEKR
Sbjct: 1   MADGISSFWGPVTSTIECCEMNYAYSSYIAEFYNTISNVPGILLALIGLVNALRQRFEKR 60

Query: 61  FSVLHISNMILAIGSMFYHATLQKVQQQGDETPMIWEMLLYMYILYSPDWHYRSTMPTFL 120
           FS+LHISNMILAIGSM YHATLQ VQQQ DETPM+WE+LLYMYILYSPDWHYRSTMPTFL
Sbjct: 61  FSILHISNMILAIGSMLYHATLQHVQQQSDETPMVWEILLYMYILYSPDWHYRSTMPTFL 120

Query: 121 FLYGAMFAVAHSILRYDIGFKVHYVILCFLCIPRMYKYYIYTEDASAKRLARLYSLTLLL 180
           FLYGA FA+ H+ LR+ IGFKVHYVILC LCIPRMYKYYI+TED +AKR+A+ Y  T+L+
Sbjct: 121 FLYGAAFAIVHAYLRFGIGFKVHYVILCLLCIPRMYKYYIHTEDTAAKRIAKWYVATILV 180

Query: 181 GTFCWVSDRGLCEKISHWIINPQGHALWHVFMGLSSYYANTFLMFCRAQQRGWSPKVLHL 240
           G+ CW  DR  C+ IS W +NPQGHALWHVFM  +SY ANTFLMFCRAQQRGW+PKV + 
Sbjct: 181 GSICWFCDRVFCKTISQWPVNPQGHALWHVFMSFNSYCANTFLMFCRAQQRGWNPKVKYF 240

Query: 241 MGVLPYVKIEKPKTQ 256
           +GVLPYVKIEKPKTQ
Sbjct: 241 LGVLPYVKIEKPKTQ 255

The following BLAST results are available for this feature:

BLAST of Chy2G027070 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94IB9	1.1e-123	80.00	Alkaline ceramidase OS=Arabidopsis thaliana OX=3702 GN=ACER PE=2 SV=1	[more]
Q9D099	6.4e-28	31.66	Alkaline ceramidase 3 OS=Mus musculus OX=10090 GN=Acer3 PE=1 SV=1	[more]
Q9NUN7	2.0e-26	31.27	Alkaline ceramidase 3 OS=Homo sapiens OX=9606 GN=ACER3 PE=1 SV=3	[more]
Q6TMJ1	1.3e-17	27.11	Putative alkaline ceramidase dcd3A OS=Dictyostelium discoideum OX=44689 GN=dcd3A...	[more]
Q9VIP7	7.3e-16	31.02	Alkaline ceramidase OS=Drosophila melanogaster OX=7227 GN=bwa PE=2 SV=2	[more]

BLAST of Chy2G027070 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3B0Z4	9.2e-147	99.22	alkaline ceramidase 3 OS=Cucumis melo OX=3656 GN=LOC103484652 PE=3 SV=1	[more]
A0A6J1KSV2	1.6e-138	92.55	alkaline ceramidase OS=Cucurbita maxima OX=3661 GN=LOC111497288 PE=3 SV=1	[more]
A0A6J1EME1	2.7e-138	92.94	alkaline ceramidase OS=Cucurbita moschata OX=3662 GN=LOC111433883 PE=3 SV=1	[more]
A0A6J1BUA9	6.6e-137	90.59	alkaline ceramidase OS=Momordica charantia OX=3673 GN=LOC111005428 PE=3 SV=1	[more]
A0A5D3CM37	2.8e-135	84.97	Alkaline ceramidase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold25...	[more]

BLAST of Chy2G027070 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008440061.1	1.56e-189	99.22	PREDICTED: alkaline ceramidase 3 [Cucumis melo] >XP_008440062.1 PREDICTED: alkal...	[more]
XP_004134791.1	1.28e-188	98.82	alkaline ceramidase [Cucumis sativus] >XP_031742854.1 alkaline ceramidase [Cucum...	[more]
XP_038882449.1	9.33e-182	94.90	alkaline ceramidase [Benincasa hispida] >XP_038882450.1 alkaline ceramidase [Ben...	[more]
XP_023003815.1	1.05e-178	92.55	alkaline ceramidase [Cucurbita maxima] >XP_023003816.1 alkaline ceramidase [Cucu...	[more]
XP_022926905.1	2.11e-178	92.94	alkaline ceramidase [Cucurbita moschata] >XP_022926906.1 alkaline ceramidase [Cu...	[more]

BLAST of Chy2G027070 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G22330.1	7.5e-125	80.00	Alkaline phytoceramidase (aPHC)	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008901	Alkaline ceramidase	PFAM	PF05875	Ceramidase	coord: 8..238 e-value: 5.8E-57 score: 193.1
None	No IPR available	PANTHER	PTHR46852:SF5	ALKALINE CERAMIDASE	coord: 1..255
IPR044219	Alkaline ceramidase, plant	PANTHER	PTHR46852	ALKALINE CERAMIDASE	coord: 1..255

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy2G027070.1	Chy2G027070.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006672	ceramide metabolic process
biological_process	GO:0009651	response to salt stress
biological_process	GO:0006914	autophagy
biological_process	GO:0010025	wax biosynthetic process
biological_process	GO:0030104	water homeostasis
biological_process	GO:0042742	defense response to bacterium
biological_process	GO:0030148	sphingolipid biosynthetic process
biological_process	GO:0031667	response to nutrient levels
biological_process	GO:0002238	response to molecule of fungal origin
biological_process	GO:0009737	response to abscisic acid
biological_process	GO:0090333	regulation of stomatal closure
biological_process	GO:0010508	positive regulation of autophagy
biological_process	GO:0010150	leaf senescence
biological_process	GO:0098542	defense response to other organism
cellular_component	GO:0005783	endoplasmic reticulum
cellular_component	GO:0005794	Golgi apparatus
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005634	nucleus
molecular_function	GO:0016811	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides