Chy2G026510 (gene) Cucumber (hystrix) v1

Overview
NameChy2G026510
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionPectinesterase
LocationchrH02: 2440962 .. 2442720 (+)
RNA-Seq ExpressionChy2G026510
SyntenyChy2G026510
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTACCCATCAGCCACTTCTTCTTCTTCAACCCCCCAAAACTTCCCGCTCCAAAACCATTCCCACAATCCTCTGTTTTGCTGCGCTTTTGTGCTCAGCCATTTTCATTACTAACAAGTTCATCAAACCAAACCCATCTGAAAATGATCTTCCCTTCCTTCAAAACATATGCCACAAGGCTTATGACCAATCATCTTGCATTGAAATGGCCGCCTCAGAATTTCCCTTGAGCATAATCAAAACCACGAACGAGGTTGATTTCTTACAGTCATTCCTGAGGAGATCCATGCCTAAAGTAATTAACACAATTGAGAGAGCCAAAGATCTCCGCCAAAGAATCAACAGCCCTCGCGGTGAAGCAGCTTTGGCCGATTGTATCGAGCTTATGGAGATTTCAAACGGCCGAATCATGGAATCTGTTTTGGCTCTGAAGAACAGAACGAGCGGCTCCATCGAAAATTCCCACACATGGCTGAGTAGCCTGCTGACAAACCACGTCACGTGCTGGGATGAGGTGGAGAGTTCATTATCGAGAGCGGCGGCAATGGATTTAGGGCTCGAGGAGTTGATTGTGAGAGGGAGAAACTCGTTGGGGATGTTGGTTTCGATTTGGGGATTGGACATTAAAAATCTGGGAGAGTTGGAGAAAAAGGGAAATGGGTATCCGAGTTGGTTGAAGAAAGGAGATCGGAGATTATTGGGAGTTTTGGGGAGAGAAATGGAACCGAATATCGTGGTGGCGAAGGATGGAAGTGGGAATTTCAAGACGGTGAAGGAGGCGGTTGAATCAGTTCCAGATAAGAGCAAGAACAGAATTGTTATATATGTGAAAAGGGGGACTTATGAGGAAAATGTGGAAGTGGGAAAGAAGAAAAAGAATGTAATGATCGTTGGAGATGGAATGGATTCCACCATTATCACCGGTAGCCTCAACGTCGTTGATGGATCAACAACTTTCAAATCTGCAACTGTAGGTAAGACTATCAGAAACACTGGTTCCTCTTTCTCTCCTAATCTTTTCATCACTTATGATCAACAACTTTCTTGTTTGATTTGATGATCCAGCTGCGGTGGGAGATGGGTTTATAGCCCAAGACATTTGGTTCCAAAACACCGCCGGGCCGGAAAAGCACCAAGCCGTGGCGCTTCGGGTCGGAGCAGATCAATCGGTCATAAACCGGTGTCGTATCGACGCTTATCAGGACACACTCTACACACATTCCAACCGCCAATTCTACCGAGATTCCACCATCACCGGCACCGTCGACTTCATATTCGGTAACGCTGCCGTCGTTTTACAGAACTGTAAAATAGAGCCCCGACGACCGATGAACAACCAAGCCAACATGGTGACGGCGCAAGGCCGAATCGACCCAAATCAGAACACTGGAACTTCGATCCAGCAATGCGATATCGTGGCAAGCTCCGACTTGGAGCCGGTTAAGAAGTCGATTAAGACATATCTGGGGCGACCATGGAAGGAGTACTCGAGGACCGTCGTCATGCAGTCCAGAATTGGCGATCTTATTCAACCAGCGGGCTGGGCCGAATGGAATGGGGACTTTGCACTGAAAACTCTCTATTACGGGGAGTATTCAAATACTGGGCCAGGCTCCGATGTTAGCAAGCGAGTGAAGTGGGCCGGTTATCATGCCATTACGAGCCCATCTGAGGCCCAAAAGTTTACGGTGAACTCACTTATACAAGGAGGTGAATGGCTCGGCCCATCGGGCGCGACTTTTATCCCAGGGCTGTGA

mRNA sequence

ATGGCTACCCATCAGCCACTTCTTCTTCTTCAACCCCCCAAAACTTCCCGCTCCAAAACCATTCCCACAATCCTCTGTTTTGCTGCGCTTTTGTGCTCAGCCATTTTCATTACTAACAAGTTCATCAAACCAAACCCATCTGAAAATGATCTTCCCTTCCTTCAAAACATATGCCACAAGGCTTATGACCAATCATCTTGCATTGAAATGGCCGCCTCAGAATTTCCCTTGAGCATAATCAAAACCACGAACGAGGTTGATTTCTTACAGTCATTCCTGAGGAGATCCATGCCTAAAGTAATTAACACAATTGAGAGAGCCAAAGATCTCCGCCAAAGAATCAACAGCCCTCGCGGTGAAGCAGCTTTGGCCGATTGTATCGAGCTTATGGAGATTTCAAACGGCCGAATCATGGAATCTGTTTTGGCTCTGAAGAACAGAACGAGCGGCTCCATCGAAAATTCCCACACATGGCTGAGTAGCCTGCTGACAAACCACGTCACGTGCTGGGATGAGGTGGAGAGTTCATTATCGAGAGCGGCGGCAATGGATTTAGGGCTCGAGGAGTTGATTGTGAGAGGGAGAAACTCGTTGGGGATGTTGGTTTCGATTTGGGGATTGGACATTAAAAATCTGGGAGAGTTGGAGAAAAAGGGAAATGGGTATCCGAGTTGGTTGAAGAAAGGAGATCGGAGATTATTGGGAGTTTTGGGGAGAGAAATGGAACCGAATATCGTGGTGGCGAAGGATGGAAGTGGGAATTTCAAGACGGTGAAGGAGGCGGTTGAATCAGTTCCAGATAAGAGCAAGAACAGAATTGTTATATATGTGAAAAGGGGGACTTATGAGGAAAATGTGGAAGTGGGAAAGAAGAAAAAGAATGTAATGATCGTTGGAGATGGAATGGATTCCACCATTATCACCGGTAGCCTCAACGTCGTTGATGGATCAACAACTTTCAAATCTGCAACTGTAGCTGCGGTGGGAGATGGGTTTATAGCCCAAGACATTTGGTTCCAAAACACCGCCGGGCCGGAAAAGCACCAAGCCGTGGCGCTTCGGGTCGGAGCAGATCAATCGGTCATAAACCGGTGTCGTATCGACGCTTATCAGGACACACTCTACACACATTCCAACCGCCAATTCTACCGAGATTCCACCATCACCGGCACCGTCGACTTCATATTCGGTAACGCTGCCGTCGTTTTACAGAACTGTAAAATAGAGCCCCGACGACCGATGAACAACCAAGCCAACATGGTGACGGCGCAAGGCCGAATCGACCCAAATCAGAACACTGGAACTTCGATCCAGCAATGCGATATCGTGGCAAGCTCCGACTTGGAGCCGGTTAAGAAGTCGATTAAGACATATCTGGGGCGACCATGGAAGGAGTACTCGAGGACCGTCGTCATGCAGTCCAGAATTGGCGATCTTATTCAACCAGCGGGCTGGGCCGAATGGAATGGGGACTTTGCACTGAAAACTCTCTATTACGGGGAGTATTCAAATACTGGGCCAGGCTCCGATGTTAGCAAGCGAGTGAAGTGGGCCGGTTATCATGCCATTACGAGCCCATCTGAGGCCCAAAAGTTTACGGTGAACTCACTTATACAAGGAGGTGAATGGCTCGGCCCATCGGGCGCGACTTTTATCCCAGGGCTGTGA

Coding sequence (CDS)

ATGGCTACCCATCAGCCACTTCTTCTTCTTCAACCCCCCAAAACTTCCCGCTCCAAAACCATTCCCACAATCCTCTGTTTTGCTGCGCTTTTGTGCTCAGCCATTTTCATTACTAACAAGTTCATCAAACCAAACCCATCTGAAAATGATCTTCCCTTCCTTCAAAACATATGCCACAAGGCTTATGACCAATCATCTTGCATTGAAATGGCCGCCTCAGAATTTCCCTTGAGCATAATCAAAACCACGAACGAGGTTGATTTCTTACAGTCATTCCTGAGGAGATCCATGCCTAAAGTAATTAACACAATTGAGAGAGCCAAAGATCTCCGCCAAAGAATCAACAGCCCTCGCGGTGAAGCAGCTTTGGCCGATTGTATCGAGCTTATGGAGATTTCAAACGGCCGAATCATGGAATCTGTTTTGGCTCTGAAGAACAGAACGAGCGGCTCCATCGAAAATTCCCACACATGGCTGAGTAGCCTGCTGACAAACCACGTCACGTGCTGGGATGAGGTGGAGAGTTCATTATCGAGAGCGGCGGCAATGGATTTAGGGCTCGAGGAGTTGATTGTGAGAGGGAGAAACTCGTTGGGGATGTTGGTTTCGATTTGGGGATTGGACATTAAAAATCTGGGAGAGTTGGAGAAAAAGGGAAATGGGTATCCGAGTTGGTTGAAGAAAGGAGATCGGAGATTATTGGGAGTTTTGGGGAGAGAAATGGAACCGAATATCGTGGTGGCGAAGGATGGAAGTGGGAATTTCAAGACGGTGAAGGAGGCGGTTGAATCAGTTCCAGATAAGAGCAAGAACAGAATTGTTATATATGTGAAAAGGGGGACTTATGAGGAAAATGTGGAAGTGGGAAAGAAGAAAAAGAATGTAATGATCGTTGGAGATGGAATGGATTCCACCATTATCACCGGTAGCCTCAACGTCGTTGATGGATCAACAACTTTCAAATCTGCAACTGTAGCTGCGGTGGGAGATGGGTTTATAGCCCAAGACATTTGGTTCCAAAACACCGCCGGGCCGGAAAAGCACCAAGCCGTGGCGCTTCGGGTCGGAGCAGATCAATCGGTCATAAACCGGTGTCGTATCGACGCTTATCAGGACACACTCTACACACATTCCAACCGCCAATTCTACCGAGATTCCACCATCACCGGCACCGTCGACTTCATATTCGGTAACGCTGCCGTCGTTTTACAGAACTGTAAAATAGAGCCCCGACGACCGATGAACAACCAAGCCAACATGGTGACGGCGCAAGGCCGAATCGACCCAAATCAGAACACTGGAACTTCGATCCAGCAATGCGATATCGTGGCAAGCTCCGACTTGGAGCCGGTTAAGAAGTCGATTAAGACATATCTGGGGCGACCATGGAAGGAGTACTCGAGGACCGTCGTCATGCAGTCCAGAATTGGCGATCTTATTCAACCAGCGGGCTGGGCCGAATGGAATGGGGACTTTGCACTGAAAACTCTCTATTACGGGGAGTATTCAAATACTGGGCCAGGCTCCGATGTTAGCAAGCGAGTGAAGTGGGCCGGTTATCATGCCATTACGAGCCCATCTGAGGCCCAAAAGTTTACGGTGAACTCACTTATACAAGGAGGTGAATGGCTCGGCCCATCGGGCGCGACTTTTATCCCAGGGCTGTGA

Protein sequence

MATHQPLLLLQPPKTSRSKTIPTILCFAALLCSAIFITNKFIKPNPSENDLPFLQNICHKAYDQSSCIEMAASEFPLSIIKTTNEVDFLQSFLRRSMPKVINTIERAKDLRQRINSPRGEAALADCIELMEISNGRIMESVLALKNRTSGSIENSHTWLSSLLTNHVTCWDEVESSLSRAAAMDLGLEELIVRGRNSLGMLVSIWGLDIKNLGELEKKGNGYPSWLKKGDRRLLGVLGREMEPNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKKNVMIVGDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVGADQSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQANMVTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVVMQSRIGDLIQPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKWAGYHAITSPSEAQKFTVNSLIQGGEWLGPSGATFIPGL*
Homology
BLAST of Chy2G026510 vs. ExPASy Swiss-Prot
Match: P83947 (Pectinesterase/pectinesterase inhibitor OS=Ficus pumila var. awkeotsang OX=204231 PE=1 SV=1)

HSP 1 Score: 594.3 bits (1531), Expect = 1.4e-168
Identity = 308/559 (55.10%), Postives = 394/559 (70.48%), Query Frame = 0

Query: 1   MATHQPLLLLQPPKTSRSKTIPTILCFAALLCSAIFITNKFIKPNPSENDLPFLQNICHK 60
           M  +QP  LL+  K+  SK    I  F  ++  A  ++  F  P     +L     IC +
Sbjct: 1   MEINQP-NLLEASKSCYSK----ITFFLLVISFAALVSTGFSSP-----ELSLHHKICDQ 60

Query: 61  AYDQSSCIEMAASEFPLSIIKTTNEVDFLQSFLRRSMPKVINTIERAKDLRQRINSPRGE 120
           + ++ SC+ M +    L++    N    L+SFL ++ P++    E A D  +RIN+P+  
Sbjct: 61  SVNKESCLAMISEVTGLNMADHRN---LLKSFLEKTTPRIQKAFETANDASRRINNPQER 120

Query: 121 AALADCIELMEISNGRIMESVLAL--KNRTSGSIENSHTWLSSLLTNHVTCWDEVESSLS 180
            AL DC ELM++S  R+++S+  L  +N T+ S E+ H WLS +LTNHVTC D +E   +
Sbjct: 121 TALLDCAELMDLSKERVVDSISILFHQNLTTRSHEDLHVWLSGVLTNHVTCLDGLEEGST 180

Query: 181 R--AAAMDLGLEELIVRGRNSLGMLVSIWGLDIKNLGELEKKGNGYPSWLKKGDRRLLGV 240
                 M+  L ELI+R R SL + V+++         +E     +P+W+  GDRRLL  
Sbjct: 181 DYIKTLMESHLNELILRARTSLAIFVTLFP---AKSNVIEPVTGNFPTWVTAGDRRLLQT 240

Query: 241 LGREMEPNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKKNVM 300
           LG+++EP+IVVAKDGSG+++T+ EAV ++PD SK R+++ V+ G YEENV+ G +KKNVM
Sbjct: 241 LGKDIEPDIVVAKDGSGDYETLNEAVAAIPDNSKKRVIVLVRTGIYEENVDFGYQKKNVM 300

Query: 301 IVGDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVG 360
           +VG+GMD TIITGS NVVDGSTTF SATVAAVGDGFIAQDI FQNTAGPEK+QAVALR+G
Sbjct: 301 LVGEGMDYTIITGSRNVVDGSTTFDSATVAAVGDGFIAQDICFQNTAGPEKYQAVALRIG 360

Query: 361 ADQSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNN 420
           AD++VINRCRIDAYQDTLY H+ RQFYRD  ITGTVDFIFGNAAVV QNC + PR+ M  
Sbjct: 361 ADETVINRCRIDAYQDTLYPHNYRQFYRDRNITGTVDFIFGNAAVVFQNCNLIPRKQMKG 420

Query: 421 QANMVTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVVMQSRI 480
           Q N +TAQGR DPNQNTGTSIQ C+I AS+DLEPV+ + K+YLGRPWKEYSRTVVM+S I
Sbjct: 421 QENTITAQGRTDPNQNTGTSIQNCEIFASADLEPVEDTFKSYLGRPWKEYSRTVVMESYI 480

Query: 481 GDLIQPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKWAGYHAITSPSEAQKFTVNS 540
            D+I PAGW EW+ DFALKTL+YGEY N GPGS  S+RVKW GYH ITSP  A++FTV  
Sbjct: 481 SDVIDPAGWLEWDRDFALKTLFYGEYRNGGPGSGTSERVKWPGYHVITSPEVAEQFTVAE 540

Query: 541 LIQGGEWLGPSGATFIPGL 556
           LIQGG WLG +G  +  GL
Sbjct: 541 LIQGGSWLGSTGVDYTAGL 543

BLAST of Chy2G026510 vs. ExPASy Swiss-Prot
Match: Q1JPL7 (Pectinesterase/pectinesterase inhibitor 18 OS=Arabidopsis thaliana OX=3702 GN=PME18 PE=1 SV=3)

HSP 1 Score: 576.6 bits (1485), Expect = 3.0e-163
Identity = 307/558 (55.02%), Postives = 387/558 (69.35%), Query Frame = 0

Query: 3   THQPLLLLQPPKTSRSKTIPTILCFAALLCSAIFITNKFIKPNPSENDLPFL--QNICHK 62
           ++QP  LL  PK+ + K +  +L F A+L S  F T + I  N + ND   L    ICH 
Sbjct: 4   SNQP--LLSKPKSLKHKNLCLVLSFVAILGSVAFFTAQLISVNTNNNDDSLLTTSQICHG 63

Query: 63  AYDQSSCIEMAASEFPLSIIKTTNEVDFLQSFLRRSMPKVINTIERAKDLRQRINSPRGE 122
           A+DQ SC +   SEF    +   N +D L  FL+ S+ ++ +T+    + R R N  R +
Sbjct: 64  AHDQDSC-QALLSEFTTLSLSKLNRLDLLHVFLKNSVWRLESTMTMVSEARIRSNGVRDK 123

Query: 123 AALADCIELMEISNGRIMESVLALK--NRTSGSIENSHTWLSSLLTNHVTCWDEV-ESSL 182
           A  ADC E+M++S  R+M S+  L+  N    S  N HTWLSS+LTN++TC + + + S+
Sbjct: 124 AGFADCEEMMDVSKDRMMSSMEELRGGNYNLESYSNVHTWLSSVLTNYMTCLESISDVSV 183

Query: 183 SRAAAMDLGLEELIVRGRNSLGMLVSIWGL--DIKNLGELEKKGNGYPSWLKKGDRRLLG 242
           +    +   LE+L+ R R +L + VS+     D+K +       N +PSWL   DR+LL 
Sbjct: 184 NSKQIVKPQLEDLVSRARVALAIFVSVLPARDDLKMI-----ISNRFPSWLTALDRKLLE 243

Query: 243 VLGREME--PNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKK 302
              + ++   N+VVAKDG+G FKTV EAV + P+ S  R VIYVK+G Y+E +++GKKKK
Sbjct: 244 SSPKTLKVTANVVVAKDGTGKFKTVNEAVAAAPENSNTRYVIYVKKGVYKETIDIGKKKK 303

Query: 303 NVMIVGDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVAL 362
           N+M+VGDG D+TIITGSLNV+DGSTTF+SATVAA GDGF+AQDIWFQNTAGP KHQAVAL
Sbjct: 304 NLMLVGDGKDATIITGSLNVIDGSTTFRSATVAANGDGFMAQDIWFQNTAGPAKHQAVAL 363

Query: 363 RVGADQSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRP 422
           RV ADQ+VINRCRIDAYQDTLYTH+ RQFYRDS ITGTVDFIFGN+AVV QNC I  R P
Sbjct: 364 RVSADQTVINRCRIDAYQDTLYTHTLRQFYRDSYITGTVDFIFGNSAVVFQNCDIVARNP 423

Query: 423 MNNQANMVTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVVMQ 482
              Q NM+TAQGR D NQNT  SIQ+C I ASSDL PVK S+KT+LGRPWK YSRTV+MQ
Sbjct: 424 GAGQKNMLTAQGREDQNQNTAISIQKCKITASSDLAPVKGSVKTFLGRPWKLYSRTVIMQ 483

Query: 483 SRIGDLIQPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKWAGYHAITSPSEAQKFT 542
           S I + I PAGW  W+G+FAL TLYYGEY+NTGPG+D SKRV W G+  I    EA++FT
Sbjct: 484 SFIDNHIDPAGWFPWDGEFALSTLYYGEYANTGPGADTSKRVNWKGFKVIKDSKEAEQFT 543

Query: 543 VNSLIQGGEWLGPSGATF 552
           V  LIQGG WL P+G TF
Sbjct: 544 VAKLIQGGLWLKPTGVTF 553

BLAST of Chy2G026510 vs. ExPASy Swiss-Prot
Match: Q96575 (Pectinesterase 2.2 OS=Solanum lycopersicum OX=4081 GN=PME2.2 PE=3 SV=1)

HSP 1 Score: 568.5 bits (1464), Expect = 8.1e-161
Identity = 304/558 (54.48%), Postives = 375/558 (67.20%), Query Frame = 0

Query: 1   MATHQPLLLLQPPKTSRSKTIPT--ILCFAALLCSAIFITNKFIKPNPSENDLPFLQNIC 60
           MAT Q  LL    KT +  +I +  IL F   L  A+F+    + P   E       N+C
Sbjct: 1   MATPQQPLL---TKTHKQNSIISFKILTFVVTLFVALFLVVFLVAPYQFEIK---HSNLC 60

Query: 61  HKAYDQSSCIEMAASEFPLSIIKTTNE-VDFLQSFLRRSMPKVINTIERAKDLRQRINSP 120
             A D   C+   +      I+ T ++ +  L  FL   + ++ N I     ++ +IN  
Sbjct: 61  KTAQDSQLCLSYVSDLMSNEIVTTDSDGLSILMKFLVNYVHQMNNAIPVVSKMKNQINDI 120

Query: 121 RGEAALADCIELMEISNGRIMESVLALKNRTSGSIENSHTWLSSLLTNHVTCWDEVESSL 180
           R E AL DC+EL++ S   + +S+ A+  RT     N+ +WLS +LTNHVTC DE++ S 
Sbjct: 121 RQEGALTDCLELLDQSVDLVSDSIAAIDKRTHSEHANAQSWLSGVLTNHVTCLDELD-SF 180

Query: 181 SRAAAMDLGLEELIVRGRNSLGMLVSIWGLDIKNLGELEKKGNGYPSWLKKGDRRLLGVL 240
           ++A      L+ELI R + +L ML S+      N   L       PSW+   DR+L+   
Sbjct: 181 TKAMINGTNLDELISRAKVALAMLASV---TTPNDDVLRPGLGKMPSWVSSRDRKLMESS 240

Query: 241 GREMEPNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKKNVMI 300
           G+++  N VVAKDG+G ++T+ EAV + PDKSK R VIYVKRG Y+ENVEV  +K  +MI
Sbjct: 241 GKDIGANAVVAKDGTGKYRTLAEAVAAAPDKSKTRYVIYVKRGIYKENVEVSSRKMKLMI 300

Query: 301 VGDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVGA 360
           VGDGM +TIITG+LNVVDGSTTF SAT+AAVG GFI QDI  QNTAGP KHQAVALRVGA
Sbjct: 301 VGDGMHATIITGNLNVVDGSTTFHSATLAAVGKGFILQDICIQNTAGPAKHQAVALRVGA 360

Query: 361 DQSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQ 420
           D+SVINRCRIDAYQDTLY HS RQFYRDS +TGT+DFIFGNAAVV Q CK+  R+P   Q
Sbjct: 361 DKSVINRCRIDAYQDTLYAHSQRQFYRDSYVTGTIDFIFGNAAVVFQKCKLVARKPGKYQ 420

Query: 421 ANMVTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVVMQSRIG 480
            NMVTAQGR DPNQ TGTSIQ C+I+ASSDLEPV K   TYLGRPWK+YSRTVVM+S +G
Sbjct: 421 QNMVTAQGRTDPNQATGTSIQFCNIIASSDLEPVLKEFPTYLGRPWKKYSRTVVMESYLG 480

Query: 481 DLIQPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKWAGYHAITSPSEAQKFTVNSL 540
            LI PAGWAEW+GDFALKTLYYGE+ N GPG+  SKRVKW GYH IT P+EA  FTV  L
Sbjct: 481 GLINPAGWAEWDGDFALKTLYYGEFMNNGPGAGTSKRVKWPGYHCITDPAEAMPFTVAKL 540

Query: 541 IQGGEWLGPSGATFIPGL 556
           IQGG WL  +G  ++ GL
Sbjct: 541 IQGGSWLRSTGVAYVDGL 548

BLAST of Chy2G026510 vs. ExPASy Swiss-Prot
Match: P09607 (Pectinesterase 2.1 OS=Solanum lycopersicum OX=4081 GN=PME2.1 PE=2 SV=2)

HSP 1 Score: 567.4 bits (1461), Expect = 1.8e-160
Identity = 301/559 (53.85%), Postives = 383/559 (68.52%), Query Frame = 0

Query: 1   MATHQPLLLLQPPKTSRSKTIPT--ILCFAALLCSAIFITNKFIKPNPSENDLPFLQNIC 60
           MAT Q  LL    KT +  +I +  IL F   L  A+F+    + P   E       N+C
Sbjct: 1   MATPQQPLL---TKTHKQNSIISFKILTFVVTLFVALFLVVFLVAPYQFEIK---HSNLC 60

Query: 61  HKAYDQSSCIEMAASEFPLSIIKTTNE-VDFLQSFLRRSMPKVINTIERAKDLRQRINSP 120
             A D   C+   +      I+ + ++ +  L+ FL  S+ ++ N I   + ++ +IN  
Sbjct: 61  KTAQDSQLCLSYVSDLISNEIVTSDSDGLSILKKFLVYSVHQMNNAIPVVRKIKNQINDI 120

Query: 121 RGEAALADCIELMEISNGRIMESVLALKNRTSGSIENSHTWLSSLLTNHVTCWDEVESSL 180
           R + AL DC+EL+++S   + +S+ A+  R+     N+ +WLS +LTNHVTC DE++ S 
Sbjct: 121 REQGALTDCLELLDLSVDLVCDSIAAIDKRSRSEHANAQSWLSGVLTNHVTCLDELD-SF 180

Query: 181 SRAAAMDLGLEELIVRGRNSLGMLVSIWGLDIKNLGELEKKGNG-YPSWLKKGDRRLLGV 240
           ++A      L+ELI R + +L ML S+   +     E+ + G G  PSW+   DR+L+  
Sbjct: 181 TKAMINGTNLDELISRAKVALAMLASVTTPN----DEVLRPGLGKMPSWVSSRDRKLMES 240

Query: 241 LGREMEPNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKKNVM 300
            G+++  N VVAKDG+G ++T+ EAV + PDKSK R VIYVKRGTY+ENVEV  +K N+M
Sbjct: 241 SGKDIGANAVVAKDGTGKYRTLAEAVAAAPDKSKTRYVIYVKRGTYKENVEVSSRKMNLM 300

Query: 301 IVGDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVG 360
           I+GDGM +TIITGSLNVVDGSTTF SAT+AAVG GFI QDI  QNTAGP KHQAVALRVG
Sbjct: 301 IIGDGMYATIITGSLNVVDGSTTFHSATLAAVGKGFILQDICIQNTAGPAKHQAVALRVG 360

Query: 361 ADQSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNN 420
           AD+SVINRCRIDAYQDTLY HS RQFYRDS +TGT+DFIFGNAAVV Q C++  R+P   
Sbjct: 361 ADKSVINRCRIDAYQDTLYAHSQRQFYRDSYVTGTIDFIFGNAAVVFQKCQLVARKPGKY 420

Query: 421 QANMVTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVVMQSRI 480
           Q NMVTAQGR DPNQ TGTSIQ CDI+AS DL+PV K   TYLGRPWK+YSRTVVM+S +
Sbjct: 421 QQNMVTAQGRTDPNQATGTSIQFCDIIASPDLKPVVKEFPTYLGRPWKKYSRTVVMESYL 480

Query: 481 GDLIQPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKWAGYHAITSPSEAQKFTVNS 540
           G LI P+GWAEW+GDFALKTLYYGE+ N GPG+  SKRVKW GYH IT P+EA  FTV  
Sbjct: 481 GGLIDPSGWAEWHGDFALKTLYYGEFMNNGPGAGTSKRVKWPGYHVITDPAEAMSFTVAK 540

Query: 541 LIQGGEWLGPSGATFIPGL 556
           LIQGG WL  +   ++ GL
Sbjct: 541 LIQGGSWLRSTDVAYVDGL 548

BLAST of Chy2G026510 vs. ExPASy Swiss-Prot
Match: P14280 (Pectinesterase 1 OS=Solanum lycopersicum OX=4081 GN=PME1.9 PE=1 SV=5)

HSP 1 Score: 555.8 bits (1431), Expect = 5.4e-157
Identity = 301/558 (53.94%), Postives = 376/558 (67.38%), Query Frame = 0

Query: 5   QPLLLLQPPKTSRSKTIPT--ILCFAALLCSAIFITNKFIKPNPSENDLPFLQNICHKAY 64
           QPLL+    KT +   I +  IL F   L  A+F+        P + ++    N+C  A 
Sbjct: 6   QPLLI----KTHKQNPIISFKILSFVITLFVALFLV------APYQVEIKH-SNLCKTAQ 65

Query: 65  DQSSCIEMAASEFPLSIIKTTNE-VDFLQSFLRRSMPKVINTIERAKDLRQRINSPRGEA 124
           D   C+   +      I+ T ++    L  FL   + ++ N I   + ++ +IN  R   
Sbjct: 66  DSQLCLSYVSDLISNEIVTTESDGHSILMKFLVNYVHQMNNAIPVVRKMKNQINDIRQHG 125

Query: 125 ALADCIELMEISNGRIMESVLALKNRTSGSIENSHTWLSSLLTNHVTCWDEVESSLSRAA 184
           AL DC+EL++ S     +S+ A+  R+     N+ +WLS +LTNHVTC DE++ S ++A 
Sbjct: 126 ALTDCLELLDQSVDFASDSIAAIDKRSRSEHANAQSWLSGVLTNHVTCLDELD-SFTKAM 185

Query: 185 AMDLGLEELIVRGRNSLGMLVSIWGLD----IKNLGELEKKGNGYPSWLKKGDRRLLGVL 244
                LEELI R + +L ML S+   D    +  LG++       PSW+   DR+L+   
Sbjct: 186 INGTNLEELISRAKVALAMLASLTTQDEDVFMTVLGKM-------PSWVSSMDRKLMESS 245

Query: 245 GREMEPNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKKNVMI 304
           G+++  N VVA+DG+G+++T+ EAV + PDKSK R VIYVKRGTY+ENVEV   K N+MI
Sbjct: 246 GKDIIANAVVAQDGTGDYQTLAEAVAAAPDKSKTRYVIYVKRGTYKENVEVASNKMNLMI 305

Query: 305 VGDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVGA 364
           VGDGM +T ITGSLNVVDGSTTF+SAT+AAVG GFI QDI  QNTAGP K QAVALRVGA
Sbjct: 306 VGDGMYATTITGSLNVVDGSTTFRSATLAAVGQGFILQDICIQNTAGPAKDQAVALRVGA 365

Query: 365 DQSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQ 424
           D SVINRCRIDAYQDTLY HS RQFYRDS +TGTVDFIFGNAAVV Q C++  R+P   Q
Sbjct: 366 DMSVINRCRIDAYQDTLYAHSQRQFYRDSYVTGTVDFIFGNAAVVFQKCQLVARKPGKYQ 425

Query: 425 ANMVTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVVMQSRIG 484
            NMVTAQGR DPNQ TGTSIQ C+I+ASSDLEPV K   TYLGRPWKEYSRTVVM+S +G
Sbjct: 426 QNMVTAQGRTDPNQATGTSIQFCNIIASSDLEPVLKEFPTYLGRPWKEYSRTVVMESYLG 485

Query: 485 DLIQPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKWAGYHAITSPSEAQKFTVNSL 544
            LI PAGWAEW+GDFALKTLYYGE+ N GPG+  SKRVKW GYH IT P++A  FTV  L
Sbjct: 486 GLINPAGWAEWDGDFALKTLYYGEFMNNGPGAGTSKRVKWPGYHVITDPAKAMPFTVAKL 544

Query: 545 IQGGEWLGPSGATFIPGL 556
           IQGG WL  +G  ++ GL
Sbjct: 546 IQGGSWLRSTGVAYVDGL 544

BLAST of Chy2G026510 vs. ExPASy TrEMBL
Match: A0A0A0KHV5 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_6G514890 PE=3 SV=1)

HSP 1 Score: 1091.3 bits (2821), Expect = 0.0e+00
Identity = 541/555 (97.48%), Postives = 551/555 (99.28%), Query Frame = 0

Query: 1   MATHQPLLLLQPPKTSRSKTIPTILCFAALLCSAIFITNKFIKPNPSENDLPFLQNICHK 60
           MATHQPLLLLQPPKTSRSKTIPT+LCFAA+LCSAIFITNKFIKPNPSENDLPFLQNICHK
Sbjct: 1   MATHQPLLLLQPPKTSRSKTIPTLLCFAAVLCSAIFITNKFIKPNPSENDLPFLQNICHK 60

Query: 61  AYDQSSCIEMAASEFPLSIIKTTNEVDFLQSFLRRSMPKVINTIERAKDLRQRINSPRGE 120
           AYD SSCIEMAASEFPLSIIKTTNEVDFLQSFLR+SMPKVI+TIERAKD+RQRINSPRGE
Sbjct: 61  AYDPSSCIEMAASEFPLSIIKTTNEVDFLQSFLRKSMPKVISTIERAKDIRQRINSPRGE 120

Query: 121 AALADCIELMEISNGRIMESVLALKNRTSGSIENSHTWLSSLLTNHVTCWDEVESSLSRA 180
           AALADCIELMEISNGRIM+SVLALKNRTSGSIENSHTWLSS+LTNHVTCWDEVESSLSRA
Sbjct: 121 AALADCIELMEISNGRIMDSVLALKNRTSGSIENSHTWLSSVLTNHVTCWDEVESSLSRA 180

Query: 181 AAMDLGLEELIVRGRNSLGMLVSIWGLDIKNLGELEKKGNGYPSWLKKGDRRLLGVLGRE 240
           A MDLGLEELI+RGRNSLGMLVSIWGLDIKNLGELEKKGNGYPSWLKKGDRRLLGVLGRE
Sbjct: 181 APMDLGLEELIMRGRNSLGMLVSIWGLDIKNLGELEKKGNGYPSWLKKGDRRLLGVLGRE 240

Query: 241 MEPNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKKNVMIVGD 300
           MEPNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKKNVMIVGD
Sbjct: 241 MEPNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKKNVMIVGD 300

Query: 301 GMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVGADQS 360
           GMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVGADQS
Sbjct: 301 GMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVGADQS 360

Query: 361 VINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQANM 420
           VINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQANM
Sbjct: 361 VINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQANM 420

Query: 421 VTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVVMQSRIGDLI 480
           VTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTV+MQSRIGDLI
Sbjct: 421 VTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVIMQSRIGDLI 480

Query: 481 QPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKWAGYHAITSPSEAQKFTVNSLIQG 540
           QPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKW GYH ITSPSEAQKFTV+SLIQG
Sbjct: 481 QPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKWDGYHIITSPSEAQKFTVDSLIQG 540

Query: 541 GEWLGPSGATFIPGL 556
           GEWLGPSGATFIPGL
Sbjct: 541 GEWLGPSGATFIPGL 555

BLAST of Chy2G026510 vs. ExPASy TrEMBL
Match: A0A5D3CNY5 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G006640 PE=3 SV=1)

HSP 1 Score: 1050.4 bits (2715), Expect = 2.6e-303
Identity = 522/555 (94.05%), Postives = 540/555 (97.30%), Query Frame = 0

Query: 1   MATHQPLLLLQPPKTSRSKTIPTILCFAALLCSAIFITNKFIKPNPSENDLPFLQNICHK 60
           MATHQPLL LQPPKTS SKTI TILCFA +LCSAIF+TNKFIK NP ENDLPFL NICHK
Sbjct: 1   MATHQPLLHLQPPKTSCSKTISTILCFAVVLCSAIFVTNKFIKLNPYENDLPFLHNICHK 60

Query: 61  AYDQSSCIEMAASEFPLSIIKTTNEVDFLQSFLRRSMPKVINTIERAKDLRQRINSPRGE 120
           AY+ SSCIEMAASEFPLSIIKTTNE  FLQSFL++SMPKVINTIERAKDLRQRINSPRGE
Sbjct: 61  AYNPSSCIEMAASEFPLSIIKTTNEEHFLQSFLKKSMPKVINTIERAKDLRQRINSPRGE 120

Query: 121 AALADCIELMEISNGRIMESVLALKNRTSGSIENSHTWLSSLLTNHVTCWDEVESSLSRA 180
            AL DCIELMEISNGRIM+SVLALKNRTSGSIE+SHTWLSS+LTNHVTCWDEVE+SLSRA
Sbjct: 121 GALDDCIELMEISNGRIMDSVLALKNRTSGSIESSHTWLSSVLTNHVTCWDEVETSLSRA 180

Query: 181 AAMDLGLEELIVRGRNSLGMLVSIWGLDIKNLGELEKKGNGYPSWLKKGDRRLLGVLGRE 240
           AAMDLGLEELI+RGRNSLGMLVSIWGL+IKNLGEL K+GNGYPSWLKKGDRRLLGVLGRE
Sbjct: 181 AAMDLGLEELIMRGRNSLGMLVSIWGLEIKNLGELGKRGNGYPSWLKKGDRRLLGVLGRE 240

Query: 241 MEPNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKKNVMIVGD 300
           MEPNIVVAKDGSGNFKTV+EAVESVPDKSK+RIVIYVK+GTY+ENVEVGKKKKNVMIVGD
Sbjct: 241 MEPNIVVAKDGSGNFKTVQEAVESVPDKSKDRIVIYVKKGTYKENVEVGKKKKNVMIVGD 300

Query: 301 GMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVGADQS 360
           GMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGP KHQAVALRVGADQS
Sbjct: 301 GMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPAKHQAVALRVGADQS 360

Query: 361 VINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQANM 420
           VINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQANM
Sbjct: 361 VINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQANM 420

Query: 421 VTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVVMQSRIGDLI 480
           VTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVVMQSRIGDLI
Sbjct: 421 VTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVVMQSRIGDLI 480

Query: 481 QPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKWAGYHAITSPSEAQKFTVNSLIQG 540
           QPAGWAEW+GDFALKTLYYGEYSNTGPGSDVSKRVKWAGYH ITSPSEAQKFTVNSLIQG
Sbjct: 481 QPAGWAEWSGDFALKTLYYGEYSNTGPGSDVSKRVKWAGYHVITSPSEAQKFTVNSLIQG 540

Query: 541 GEWLGPSGATFIPGL 556
           GEWLGPSGA+FIPGL
Sbjct: 541 GEWLGPSGASFIPGL 555

BLAST of Chy2G026510 vs. ExPASy TrEMBL
Match: A0A1S3B018 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103484595 PE=3 SV=1)

HSP 1 Score: 1048.9 bits (2711), Expect = 7.5e-303
Identity = 522/555 (94.05%), Postives = 538/555 (96.94%), Query Frame = 0

Query: 1   MATHQPLLLLQPPKTSRSKTIPTILCFAALLCSAIFITNKFIKPNPSENDLPFLQNICHK 60
           MATHQPLL LQPPKTS SKTI TILCFA +LCSAIF+TNKFIK NP ENDLPFL NICHK
Sbjct: 1   MATHQPLLHLQPPKTSCSKTISTILCFAVVLCSAIFVTNKFIKLNPYENDLPFLHNICHK 60

Query: 61  AYDQSSCIEMAASEFPLSIIKTTNEVDFLQSFLRRSMPKVINTIERAKDLRQRINSPRGE 120
           AY+ SSCIEMAASEFPLSIIKTTNE  FLQSFL++SMPKVINTIERAKDLRQRINSPRGE
Sbjct: 61  AYNPSSCIEMAASEFPLSIIKTTNEEHFLQSFLKKSMPKVINTIERAKDLRQRINSPRGE 120

Query: 121 AALADCIELMEISNGRIMESVLALKNRTSGSIENSHTWLSSLLTNHVTCWDEVESSLSRA 180
            AL DCIELMEISNGRIM+SVLALKNRTSG IE+SHTWLSS+LTNHVTCWDEVESSLSRA
Sbjct: 121 GALDDCIELMEISNGRIMDSVLALKNRTSGYIESSHTWLSSVLTNHVTCWDEVESSLSRA 180

Query: 181 AAMDLGLEELIVRGRNSLGMLVSIWGLDIKNLGELEKKGNGYPSWLKKGDRRLLGVLGRE 240
           AAMDLGLEELI+RGRNSLGMLVSIWGL+IKNLGEL K+GNGYPSWLKKGDRRLLGVLGRE
Sbjct: 181 AAMDLGLEELIMRGRNSLGMLVSIWGLEIKNLGELGKRGNGYPSWLKKGDRRLLGVLGRE 240

Query: 241 MEPNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKKNVMIVGD 300
           MEPNIVVAKDGSGNFKTV+EAVESVPDKSKNRIVIYVK+GTY+ENVEVGKKKKNVMIVGD
Sbjct: 241 MEPNIVVAKDGSGNFKTVQEAVESVPDKSKNRIVIYVKKGTYKENVEVGKKKKNVMIVGD 300

Query: 301 GMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVGADQS 360
           GMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGP KHQAVALRVGADQS
Sbjct: 301 GMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPAKHQAVALRVGADQS 360

Query: 361 VINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQANM 420
           VINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQANM
Sbjct: 361 VINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQANM 420

Query: 421 VTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVVMQSRIGDLI 480
           VTAQGRIDPNQNTGTSIQQCDIVAS DLEPVKKSIKTYLGRPWKEYSRTVVMQSRIGDLI
Sbjct: 421 VTAQGRIDPNQNTGTSIQQCDIVASFDLEPVKKSIKTYLGRPWKEYSRTVVMQSRIGDLI 480

Query: 481 QPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKWAGYHAITSPSEAQKFTVNSLIQG 540
           QPAGWAEW+GDFALKTLYYGEYSNTGPGSDVSKRVKWAGYH ITSPSEAQKFTVNSLIQG
Sbjct: 481 QPAGWAEWSGDFALKTLYYGEYSNTGPGSDVSKRVKWAGYHVITSPSEAQKFTVNSLIQG 540

Query: 541 GEWLGPSGATFIPGL 556
           GEWLGPSGA+FIPGL
Sbjct: 541 GEWLGPSGASFIPGL 555

BLAST of Chy2G026510 vs. ExPASy TrEMBL
Match: A0A6J1KRW2 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111496691 PE=3 SV=1)

HSP 1 Score: 888.6 bits (2295), Expect = 1.3e-254
Identity = 450/557 (80.79%), Postives = 495/557 (88.87%), Query Frame = 0

Query: 1   MATHQPLLLLQPPKTSRSKTIPTILCFAALLCSAIFITNKFIKPNPSEND-LPFLQNICH 60
           MATH+P LL QPP+T+R KT  TIL F+ LL SAIFITNK I  NPSEND  PFL NIC+
Sbjct: 1   MATHEP-LLPQPPETTRPKTFFTILFFSLLLGSAIFITNKLINSNPSENDHHPFLHNICY 60

Query: 61  KAYDQSSCIEMAASEFP-LSIIKTTNEVDFLQSFLRRSMPKVINTIERAKDLRQRINSPR 120
           KAY+QSSCI+M ASEFP L   KTTN  +FLQSFLR+S  K++NTIE AKDLR+RIN+PR
Sbjct: 61  KAYNQSSCIQMLASEFPLLQETKTTNAANFLQSFLRKSTSKIVNTIESAKDLRRRINNPR 120

Query: 121 GEAALADCIELMEISNGRIMESVLALKNRTSGSIENSHTWLSSLLTNHVTCWDEVESSLS 180
           GEAALADC+ELMEIS  RI +S+LAL+ R  G+IEN+HTWLSS+LTNHV C DE+E+S+S
Sbjct: 121 GEAALADCVELMEISMDRITDSILALERRRCGTIENAHTWLSSVLTNHVACSDEIENSIS 180

Query: 181 RAAAMDLGLEELIVRGRNSLGMLVSIWGLDIKNLGELEKKGNGYPSWLKKGDRRLLGVLG 240
           +AAAMDLG+EELIV GRNSLGMLVSIWG         +K+G GYPSWLKKGDRRLL V+G
Sbjct: 181 KAAAMDLGVEELIVIGRNSLGMLVSIWGSG-------KKEGMGYPSWLKKGDRRLLEVVG 240

Query: 241 REMEPNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKKNVMIV 300
           R M+ +IVVAKDGSGNFKTVKEAVE+VPDKSK+RIVIYVK+G Y+ENVEVGK KKNVM+V
Sbjct: 241 RGMKADIVVAKDGSGNFKTVKEAVEAVPDKSKSRIVIYVKKGIYKENVEVGKNKKNVMLV 300

Query: 301 GDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVGAD 360
           GDGMDSTIIT SLNVVDGSTTFKSATVAAVGDGFIAQDIWF+NTA PEKHQAVALRVGAD
Sbjct: 301 GDGMDSTIITASLNVVDGSTTFKSATVAAVGDGFIAQDIWFRNTAEPEKHQAVALRVGAD 360

Query: 361 QSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQA 420
           QSVINRCRIDAYQDTLY H+NRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPM+NQA
Sbjct: 361 QSVINRCRIDAYQDTLYAHTNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMSNQA 420

Query: 421 NMVTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVVMQSRIGD 480
           NMVTAQGR DPNQNTGTSIQ+C+IVAS DLEPVKKSIKTYLGRPWKEYSRTVVMQS +GD
Sbjct: 421 NMVTAQGRTDPNQNTGTSIQRCEIVASPDLEPVKKSIKTYLGRPWKEYSRTVVMQSTLGD 480

Query: 481 LIQPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKWAGYHAITSPSEAQKFTVNSLI 540
           LI PAGWAEWNG+FALKTLYYGEYSN GPGS+VSKRVKW GYH ITSP+ AQKFTV+SLI
Sbjct: 481 LIDPAGWAEWNGEFALKTLYYGEYSNNGPGSNVSKRVKWPGYHVITSPAVAQKFTVDSLI 540

Query: 541 QGGEWLGPSGATFIPGL 556
           QG EWLG SG TFIPGL
Sbjct: 541 QGAEWLGSSGVTFIPGL 549

BLAST of Chy2G026510 vs. ExPASy TrEMBL
Match: A0A6J1CN37 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111012508 PE=3 SV=1)

HSP 1 Score: 840.5 bits (2170), Expect = 4.1e-240
Identity = 436/561 (77.72%), Postives = 473/561 (84.31%), Query Frame = 0

Query: 1   MATHQPLLLLQPPKTSRSKTIPTILCFAALLCSAIFITNKFIKPNPSENDLPFLQNICHK 60
           MAT+QP  LLQPPKT RSK    ILCFA +LCSA FI N  I PNPSE D  FL NIC K
Sbjct: 1   MATNQP--LLQPPKTHRSKAFSIILCFATILCSAFFIANHLIGPNPSEIDQLFLHNICRK 60

Query: 61  AYDQSSCIEMAASEFPL-SIIKTTNEVDFLQSFLRRSMPKVINTIERAKD--LRQRINSP 120
           AY+ +SC+ MAASE P     K TN V FLQ FLR+S+ KV +TIERA D  L +RIN  
Sbjct: 61  AYNPASCVAMAASEVPFHREAKMTNGVHFLQVFLRKSLTKVTDTIERANDLILSRRINDA 120

Query: 121 RGEAALADCIELMEISNGRIMESV--LALKNRTSGSIENSHTWLSSLLTNHVTCWDEVES 180
           RGEAALADC+ELMEIS  RI +S+  LAL N+TS  IE++H WLSS+LTNHVTC DE+E 
Sbjct: 121 RGEAALADCLELMEISMDRITDSILALALNNKTSVPIEDAHAWLSSVLTNHVTCSDELEK 180

Query: 181 SLSRAAAMDLGLEELIVRGRNSLGMLVSIWGLDIKNLGELEKKGN-GYPSWLKKGDRRLL 240
           SL    A +LGLEELI+  RNSL MLVSI     +   E E +G  G+P WLK GDR LL
Sbjct: 181 SLLGTTAAELGLEELIMTARNSLAMLVSI----SQPEPEPEIEGQIGFPGWLKSGDRMLL 240

Query: 241 GVLGREMEPNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKKN 300
           GV G++++ NIVVAKDGSGNFKTVKEAV SVPDKSKNR VIYVK+GTYEENVEV KKKKN
Sbjct: 241 GVTGQQIKANIVVAKDGSGNFKTVKEAVASVPDKSKNRFVIYVKKGTYEENVEVEKKKKN 300

Query: 301 VMIVGDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALR 360
           VMIVGDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWF+NTAGPEKHQAVALR
Sbjct: 301 VMIVGDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFRNTAGPEKHQAVALR 360

Query: 361 VGADQSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPM 420
           VGADQSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIE RRPM
Sbjct: 361 VGADQSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEARRPM 420

Query: 421 NNQANMVTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVVMQS 480
           +NQANMVTAQGRIDPNQNTGTSI +C IVASSDLEPVK+SIKTYLGRPWKEYSRTV+MQS
Sbjct: 421 SNQANMVTAQGRIDPNQNTGTSIHRCQIVASSDLEPVKRSIKTYLGRPWKEYSRTVIMQS 480

Query: 481 RIGDLIQPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKWAGYHAITSPSEAQKFTV 540
           RIGDLI P GWAEWNGDFALKTLYYGEY N GPGSDVSKRVKW GY  IT+P+EAQKFTV
Sbjct: 481 RIGDLIDPTGWAEWNGDFALKTLYYGEYLNNGPGSDVSKRVKWPGYRVITNPAEAQKFTV 540

Query: 541 NSLIQGGEWLGPSGATFIPGL 556
           +SLIQGG+WLGP+G TFIPGL
Sbjct: 541 DSLIQGGDWLGPTGVTFIPGL 555

BLAST of Chy2G026510 vs. NCBI nr
Match: XP_004134752.1 (pectinesterase [Cucumis sativus] >KGN49128.1 hypothetical protein Csa_004290 [Cucumis sativus])

HSP 1 Score: 1088 bits (2815), Expect = 0.0
Identity = 541/555 (97.48%), Postives = 551/555 (99.28%), Query Frame = 0

Query: 1   MATHQPLLLLQPPKTSRSKTIPTILCFAALLCSAIFITNKFIKPNPSENDLPFLQNICHK 60
           MATHQPLLLLQPPKTSRSKTIPT+LCFAA+LCSAIFITNKFIKPNPSENDLPFLQNICHK
Sbjct: 1   MATHQPLLLLQPPKTSRSKTIPTLLCFAAVLCSAIFITNKFIKPNPSENDLPFLQNICHK 60

Query: 61  AYDQSSCIEMAASEFPLSIIKTTNEVDFLQSFLRRSMPKVINTIERAKDLRQRINSPRGE 120
           AYD SSCIEMAASEFPLSIIKTTNEVDFLQSFLR+SMPKVI+TIERAKD+RQRINSPRGE
Sbjct: 61  AYDPSSCIEMAASEFPLSIIKTTNEVDFLQSFLRKSMPKVISTIERAKDIRQRINSPRGE 120

Query: 121 AALADCIELMEISNGRIMESVLALKNRTSGSIENSHTWLSSLLTNHVTCWDEVESSLSRA 180
           AALADCIELMEISNGRIM+SVLALKNRTSGSIENSHTWLSS+LTNHVTCWDEVESSLSRA
Sbjct: 121 AALADCIELMEISNGRIMDSVLALKNRTSGSIENSHTWLSSVLTNHVTCWDEVESSLSRA 180

Query: 181 AAMDLGLEELIVRGRNSLGMLVSIWGLDIKNLGELEKKGNGYPSWLKKGDRRLLGVLGRE 240
           A MDLGLEELI+RGRNSLGMLVSIWGLDIKNLGELEKKGNGYPSWLKKGDRRLLGVLGRE
Sbjct: 181 APMDLGLEELIMRGRNSLGMLVSIWGLDIKNLGELEKKGNGYPSWLKKGDRRLLGVLGRE 240

Query: 241 MEPNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKKNVMIVGD 300
           MEPNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKKNVMIVGD
Sbjct: 241 MEPNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKKNVMIVGD 300

Query: 301 GMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVGADQS 360
           GMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVGADQS
Sbjct: 301 GMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVGADQS 360

Query: 361 VINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQANM 420
           VINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQANM
Sbjct: 361 VINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQANM 420

Query: 421 VTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVVMQSRIGDLI 480
           VTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTV+MQSRIGDLI
Sbjct: 421 VTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVIMQSRIGDLI 480

Query: 481 QPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKWAGYHAITSPSEAQKFTVNSLIQG 540
           QPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKW GYH ITSPSEAQKFTV+SLIQG
Sbjct: 481 QPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKWDGYHIITSPSEAQKFTVDSLIQG 540

Query: 541 GEWLGPSGATFIPGL 555
           GEWLGPSGATFIPGL
Sbjct: 541 GEWLGPSGATFIPGL 555

BLAST of Chy2G026510 vs. NCBI nr
Match: TYK13062.1 (pectinesterase [Cucumis melo var. makuwa])

HSP 1 Score: 1048 bits (2709), Expect = 0.0
Identity = 522/555 (94.05%), Postives = 540/555 (97.30%), Query Frame = 0

Query: 1   MATHQPLLLLQPPKTSRSKTIPTILCFAALLCSAIFITNKFIKPNPSENDLPFLQNICHK 60
           MATHQPLL LQPPKTS SKTI TILCFA +LCSAIF+TNKFIK NP ENDLPFL NICHK
Sbjct: 1   MATHQPLLHLQPPKTSCSKTISTILCFAVVLCSAIFVTNKFIKLNPYENDLPFLHNICHK 60

Query: 61  AYDQSSCIEMAASEFPLSIIKTTNEVDFLQSFLRRSMPKVINTIERAKDLRQRINSPRGE 120
           AY+ SSCIEMAASEFPLSIIKTTNE  FLQSFL++SMPKVINTIERAKDLRQRINSPRGE
Sbjct: 61  AYNPSSCIEMAASEFPLSIIKTTNEEHFLQSFLKKSMPKVINTIERAKDLRQRINSPRGE 120

Query: 121 AALADCIELMEISNGRIMESVLALKNRTSGSIENSHTWLSSLLTNHVTCWDEVESSLSRA 180
            AL DCIELMEISNGRIM+SVLALKNRTSGSIE+SHTWLSS+LTNHVTCWDEVE+SLSRA
Sbjct: 121 GALDDCIELMEISNGRIMDSVLALKNRTSGSIESSHTWLSSVLTNHVTCWDEVETSLSRA 180

Query: 181 AAMDLGLEELIVRGRNSLGMLVSIWGLDIKNLGELEKKGNGYPSWLKKGDRRLLGVLGRE 240
           AAMDLGLEELI+RGRNSLGMLVSIWGL+IKNLGEL K+GNGYPSWLKKGDRRLLGVLGRE
Sbjct: 181 AAMDLGLEELIMRGRNSLGMLVSIWGLEIKNLGELGKRGNGYPSWLKKGDRRLLGVLGRE 240

Query: 241 MEPNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKKNVMIVGD 300
           MEPNIVVAKDGSGNFKTV+EAVESVPDKSK+RIVIYVK+GTY+ENVEVGKKKKNVMIVGD
Sbjct: 241 MEPNIVVAKDGSGNFKTVQEAVESVPDKSKDRIVIYVKKGTYKENVEVGKKKKNVMIVGD 300

Query: 301 GMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVGADQS 360
           GMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGP KHQAVALRVGADQS
Sbjct: 301 GMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPAKHQAVALRVGADQS 360

Query: 361 VINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQANM 420
           VINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQANM
Sbjct: 361 VINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQANM 420

Query: 421 VTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVVMQSRIGDLI 480
           VTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVVMQSRIGDLI
Sbjct: 421 VTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVVMQSRIGDLI 480

Query: 481 QPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKWAGYHAITSPSEAQKFTVNSLIQG 540
           QPAGWAEW+GDFALKTLYYGEYSNTGPGSDVSKRVKWAGYH ITSPSEAQKFTVNSLIQG
Sbjct: 481 QPAGWAEWSGDFALKTLYYGEYSNTGPGSDVSKRVKWAGYHVITSPSEAQKFTVNSLIQG 540

Query: 541 GEWLGPSGATFIPGL 555
           GEWLGPSGA+FIPGL
Sbjct: 541 GEWLGPSGASFIPGL 555

BLAST of Chy2G026510 vs. NCBI nr
Match: XP_008439965.1 (PREDICTED: pectinesterase [Cucumis melo])

HSP 1 Score: 1046 bits (2705), Expect = 0.0
Identity = 522/555 (94.05%), Postives = 538/555 (96.94%), Query Frame = 0

Query: 1   MATHQPLLLLQPPKTSRSKTIPTILCFAALLCSAIFITNKFIKPNPSENDLPFLQNICHK 60
           MATHQPLL LQPPKTS SKTI TILCFA +LCSAIF+TNKFIK NP ENDLPFL NICHK
Sbjct: 1   MATHQPLLHLQPPKTSCSKTISTILCFAVVLCSAIFVTNKFIKLNPYENDLPFLHNICHK 60

Query: 61  AYDQSSCIEMAASEFPLSIIKTTNEVDFLQSFLRRSMPKVINTIERAKDLRQRINSPRGE 120
           AY+ SSCIEMAASEFPLSIIKTTNE  FLQSFL++SMPKVINTIERAKDLRQRINSPRGE
Sbjct: 61  AYNPSSCIEMAASEFPLSIIKTTNEEHFLQSFLKKSMPKVINTIERAKDLRQRINSPRGE 120

Query: 121 AALADCIELMEISNGRIMESVLALKNRTSGSIENSHTWLSSLLTNHVTCWDEVESSLSRA 180
            AL DCIELMEISNGRIM+SVLALKNRTSG IE+SHTWLSS+LTNHVTCWDEVESSLSRA
Sbjct: 121 GALDDCIELMEISNGRIMDSVLALKNRTSGYIESSHTWLSSVLTNHVTCWDEVESSLSRA 180

Query: 181 AAMDLGLEELIVRGRNSLGMLVSIWGLDIKNLGELEKKGNGYPSWLKKGDRRLLGVLGRE 240
           AAMDLGLEELI+RGRNSLGMLVSIWGL+IKNLGEL K+GNGYPSWLKKGDRRLLGVLGRE
Sbjct: 181 AAMDLGLEELIMRGRNSLGMLVSIWGLEIKNLGELGKRGNGYPSWLKKGDRRLLGVLGRE 240

Query: 241 MEPNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKKNVMIVGD 300
           MEPNIVVAKDGSGNFKTV+EAVESVPDKSKNRIVIYVK+GTY+ENVEVGKKKKNVMIVGD
Sbjct: 241 MEPNIVVAKDGSGNFKTVQEAVESVPDKSKNRIVIYVKKGTYKENVEVGKKKKNVMIVGD 300

Query: 301 GMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVGADQS 360
           GMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGP KHQAVALRVGADQS
Sbjct: 301 GMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPAKHQAVALRVGADQS 360

Query: 361 VINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQANM 420
           VINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQANM
Sbjct: 361 VINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQANM 420

Query: 421 VTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVVMQSRIGDLI 480
           VTAQGRIDPNQNTGTSIQQCDIVAS DLEPVKKSIKTYLGRPWKEYSRTVVMQSRIGDLI
Sbjct: 421 VTAQGRIDPNQNTGTSIQQCDIVASFDLEPVKKSIKTYLGRPWKEYSRTVVMQSRIGDLI 480

Query: 481 QPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKWAGYHAITSPSEAQKFTVNSLIQG 540
           QPAGWAEW+GDFALKTLYYGEYSNTGPGSDVSKRVKWAGYH ITSPSEAQKFTVNSLIQG
Sbjct: 481 QPAGWAEWSGDFALKTLYYGEYSNTGPGSDVSKRVKWAGYHVITSPSEAQKFTVNSLIQG 540

Query: 541 GEWLGPSGATFIPGL 555
           GEWLGPSGA+FIPGL
Sbjct: 541 GEWLGPSGASFIPGL 555

BLAST of Chy2G026510 vs. NCBI nr
Match: XP_038881670.1 (pectinesterase [Benincasa hispida])

HSP 1 Score: 993 bits (2568), Expect = 0.0
Identity = 496/556 (89.21%), Postives = 526/556 (94.60%), Query Frame = 0

Query: 1   MATHQPLLLLQPPKTSRSKTIPTILCFAALLCSAIFITNKFIKPNPSENDLPFLQNICHK 60
           MATHQPLL  QPPKTSRSKTIPTILCFAA+LCSAIFITNKFIK N SENDL FL N+CHK
Sbjct: 1   MATHQPLL--QPPKTSRSKTIPTILCFAAVLCSAIFITNKFIKSNSSENDLSFLHNLCHK 60

Query: 61  AYDQSSCIEMAASEFPLSI-IKTTNEVDFLQSFLRRSMPKVINTIERAKDLRQRINSPRG 120
           AY+ SSCIEMAASEFP    IKTTN   FLQSFLR+S+PKVINTI+RAKDL QRIN+PRG
Sbjct: 61  AYNPSSCIEMAASEFPFPREIKTTNRGHFLQSFLRKSIPKVINTIDRAKDLSQRINNPRG 120

Query: 121 EAALADCIELMEISNGRIMESVLALKNRTSGSIENSHTWLSSLLTNHVTCWDEVESSLSR 180
           EAALADC+ELMEISN RI++SV ALKNRTSGSIEN+HTWLSS+LTNH+TCWDEVE+S+SR
Sbjct: 121 EAALADCVELMEISNSRIIDSVSALKNRTSGSIENAHTWLSSVLTNHITCWDEVENSVSR 180

Query: 181 AAAMDLGLEELIVRGRNSLGMLVSIWGLDIKNLGELEKKGNGYPSWLKKGDRRLLGVLGR 240
           AAAMDLGLEELI+RGRNSLGM VSIW  +I+N+GELEK+ NGYPSWLKKGDRRLLGV G+
Sbjct: 181 AAAMDLGLEELIMRGRNSLGMFVSIWESEIENMGELEKRVNGYPSWLKKGDRRLLGVPGK 240

Query: 241 EMEPNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKKNVMIVG 300
           EME NIVVAKDGSGNF TV EAVESVPDKSKNRIVIYVK+G Y+E VEVGKKKKNVMIVG
Sbjct: 241 EMEANIVVAKDGSGNFMTVTEAVESVPDKSKNRIVIYVKKGIYKEKVEVGKKKKNVMIVG 300

Query: 301 DGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVGADQ 360
           DGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVGADQ
Sbjct: 301 DGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVGADQ 360

Query: 361 SVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQAN 420
           SVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPM+NQAN
Sbjct: 361 SVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMSNQAN 420

Query: 421 MVTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVVMQSRIGDL 480
           MVTAQGR+DPNQNTG+SIQ+C+IVASSDLEPVK+SIKTYLGRPWKEYSRTVVMQS IGDL
Sbjct: 421 MVTAQGRMDPNQNTGSSIQKCEIVASSDLEPVKQSIKTYLGRPWKEYSRTVVMQSTIGDL 480

Query: 481 IQPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKWAGYHAITSPSEAQKFTVNSLIQ 540
           IQPAGWAEWNGDFALKTLYYGEYSN GPGSDVSKRVKWAGYH ITSPSEAQKFTV+SLIQ
Sbjct: 481 IQPAGWAEWNGDFALKTLYYGEYSNNGPGSDVSKRVKWAGYHVITSPSEAQKFTVDSLIQ 540

Query: 541 GGEWLGPSGATFIPGL 555
           GGEWLGPSGATFIPGL
Sbjct: 541 GGEWLGPSGATFIPGL 554

BLAST of Chy2G026510 vs. NCBI nr
Match: XP_023002934.1 (pectinesterase-like [Cucurbita maxima])

HSP 1 Score: 886 bits (2290), Expect = 0.0
Identity = 450/557 (80.79%), Postives = 495/557 (88.87%), Query Frame = 0

Query: 1   MATHQPLLLLQPPKTSRSKTIPTILCFAALLCSAIFITNKFIKPNPSENDL-PFLQNICH 60
           MATH+PLL  QPP+T+R KT  TIL F+ LL SAIFITNK I  NPSEND  PFL NIC+
Sbjct: 1   MATHEPLLP-QPPETTRPKTFFTILFFSLLLGSAIFITNKLINSNPSENDHHPFLHNICY 60

Query: 61  KAYDQSSCIEMAASEFPL-SIIKTTNEVDFLQSFLRRSMPKVINTIERAKDLRQRINSPR 120
           KAY+QSSCI+M ASEFPL    KTTN  +FLQSFLR+S  K++NTIE AKDLR+RIN+PR
Sbjct: 61  KAYNQSSCIQMLASEFPLLQETKTTNAANFLQSFLRKSTSKIVNTIESAKDLRRRINNPR 120

Query: 121 GEAALADCIELMEISNGRIMESVLALKNRTSGSIENSHTWLSSLLTNHVTCWDEVESSLS 180
           GEAALADC+ELMEIS  RI +S+LAL+ R  G+IEN+HTWLSS+LTNHV C DE+E+S+S
Sbjct: 121 GEAALADCVELMEISMDRITDSILALERRRCGTIENAHTWLSSVLTNHVACSDEIENSIS 180

Query: 181 RAAAMDLGLEELIVRGRNSLGMLVSIWGLDIKNLGELEKKGNGYPSWLKKGDRRLLGVLG 240
           +AAAMDLG+EELIV GRNSLGMLVSIWG         +K+G GYPSWLKKGDRRLL V+G
Sbjct: 181 KAAAMDLGVEELIVIGRNSLGMLVSIWGSG-------KKEGMGYPSWLKKGDRRLLEVVG 240

Query: 241 REMEPNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKKNVMIV 300
           R M+ +IVVAKDGSGNFKTVKEAVE+VPDKSK+RIVIYVK+G Y+ENVEVGK KKNVM+V
Sbjct: 241 RGMKADIVVAKDGSGNFKTVKEAVEAVPDKSKSRIVIYVKKGIYKENVEVGKNKKNVMLV 300

Query: 301 GDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVGAD 360
           GDGMDSTIIT SLNVVDGSTTFKSATVAAVGDGFIAQDIWF+NTA PEKHQAVALRVGAD
Sbjct: 301 GDGMDSTIITASLNVVDGSTTFKSATVAAVGDGFIAQDIWFRNTAEPEKHQAVALRVGAD 360

Query: 361 QSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQA 420
           QSVINRCRIDAYQDTLY H+NRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPM+NQA
Sbjct: 361 QSVINRCRIDAYQDTLYAHTNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMSNQA 420

Query: 421 NMVTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVVMQSRIGD 480
           NMVTAQGR DPNQNTGTSIQ+C+IVAS DLEPVKKSIKTYLGRPWKEYSRTVVMQS +GD
Sbjct: 421 NMVTAQGRTDPNQNTGTSIQRCEIVASPDLEPVKKSIKTYLGRPWKEYSRTVVMQSTLGD 480

Query: 481 LIQPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKWAGYHAITSPSEAQKFTVNSLI 540
           LI PAGWAEWNG+FALKTLYYGEYSN GPGS+VSKRVKW GYH ITSP+ AQKFTV+SLI
Sbjct: 481 LIDPAGWAEWNGEFALKTLYYGEYSNNGPGSNVSKRVKWPGYHVITSPAVAQKFTVDSLI 540

Query: 541 QGGEWLGPSGATFIPGL 555
           QG EWLG SG TFIPGL
Sbjct: 541 QGAEWLGSSGVTFIPGL 549

BLAST of Chy2G026510 vs. TAIR 10
Match: AT1G11580.1 (methylesterase PCR A )

HSP 1 Score: 576.6 bits (1485), Expect = 2.1e-164
Identity = 307/558 (55.02%), Postives = 387/558 (69.35%), Query Frame = 0

Query: 3   THQPLLLLQPPKTSRSKTIPTILCFAALLCSAIFITNKFIKPNPSENDLPFL--QNICHK 62
           ++QP  LL  PK+ + K +  +L F A+L S  F T + I  N + ND   L    ICH 
Sbjct: 4   SNQP--LLSKPKSLKHKNLCLVLSFVAILGSVAFFTAQLISVNTNNNDDSLLTTSQICHG 63

Query: 63  AYDQSSCIEMAASEFPLSIIKTTNEVDFLQSFLRRSMPKVINTIERAKDLRQRINSPRGE 122
           A+DQ SC +   SEF    +   N +D L  FL+ S+ ++ +T+    + R R N  R +
Sbjct: 64  AHDQDSC-QALLSEFTTLSLSKLNRLDLLHVFLKNSVWRLESTMTMVSEARIRSNGVRDK 123

Query: 123 AALADCIELMEISNGRIMESVLALK--NRTSGSIENSHTWLSSLLTNHVTCWDEV-ESSL 182
           A  ADC E+M++S  R+M S+  L+  N    S  N HTWLSS+LTN++TC + + + S+
Sbjct: 124 AGFADCEEMMDVSKDRMMSSMEELRGGNYNLESYSNVHTWLSSVLTNYMTCLESISDVSV 183

Query: 183 SRAAAMDLGLEELIVRGRNSLGMLVSIWGL--DIKNLGELEKKGNGYPSWLKKGDRRLLG 242
           +    +   LE+L+ R R +L + VS+     D+K +       N +PSWL   DR+LL 
Sbjct: 184 NSKQIVKPQLEDLVSRARVALAIFVSVLPARDDLKMI-----ISNRFPSWLTALDRKLLE 243

Query: 243 VLGREME--PNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKK 302
              + ++   N+VVAKDG+G FKTV EAV + P+ S  R VIYVK+G Y+E +++GKKKK
Sbjct: 244 SSPKTLKVTANVVVAKDGTGKFKTVNEAVAAAPENSNTRYVIYVKKGVYKETIDIGKKKK 303

Query: 303 NVMIVGDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVAL 362
           N+M+VGDG D+TIITGSLNV+DGSTTF+SATVAA GDGF+AQDIWFQNTAGP KHQAVAL
Sbjct: 304 NLMLVGDGKDATIITGSLNVIDGSTTFRSATVAANGDGFMAQDIWFQNTAGPAKHQAVAL 363

Query: 363 RVGADQSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRP 422
           RV ADQ+VINRCRIDAYQDTLYTH+ RQFYRDS ITGTVDFIFGN+AVV QNC I  R P
Sbjct: 364 RVSADQTVINRCRIDAYQDTLYTHTLRQFYRDSYITGTVDFIFGNSAVVFQNCDIVARNP 423

Query: 423 MNNQANMVTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVVMQ 482
              Q NM+TAQGR D NQNT  SIQ+C I ASSDL PVK S+KT+LGRPWK YSRTV+MQ
Sbjct: 424 GAGQKNMLTAQGREDQNQNTAISIQKCKITASSDLAPVKGSVKTFLGRPWKLYSRTVIMQ 483

Query: 483 SRIGDLIQPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKWAGYHAITSPSEAQKFT 542
           S I + I PAGW  W+G+FAL TLYYGEY+NTGPG+D SKRV W G+  I    EA++FT
Sbjct: 484 SFIDNHIDPAGWFPWDGEFALSTLYYGEYANTGPGADTSKRVNWKGFKVIKDSKEAEQFT 543

Query: 543 VNSLIQGGEWLGPSGATF 552
           V  LIQGG WL P+G TF
Sbjct: 544 VAKLIQGGLWLKPTGVTF 553

BLAST of Chy2G026510 vs. TAIR 10
Match: AT3G14310.1 (pectin methylesterase 3 )

HSP 1 Score: 439.9 bits (1130), Expect = 3.1e-123
Identity = 254/537 (47.30%), Postives = 321/537 (59.78%), Query Frame = 0

Query: 64  QSSCIEMAASEFPLSIIKT------TNEVDFLQSFLRRSMPKVINTIERAKDL--RQRIN 123
           +SSC      E  +S + T      T++ D +++ +  ++  V +     K L  +++  
Sbjct: 60  RSSCSSTRYPELCISAVVTAGGVELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGL 119

Query: 124 SPRGEAALADCIELMEISNGRIMESVLALKNRTSGSIENSH-----TWLSSLLTNHVTCW 183
           +PR + AL DC+E ++ +   + E+V  L    +      H     T +SS +TN  TC 
Sbjct: 120 TPREKTALHDCLETIDETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQETCL 179

Query: 184 D--------------------EVESSLSRAAAMDLGLEELIVRGRNSLGMLVS------- 243
           D                     VE   S A AM   + +  +        + S       
Sbjct: 180 DGFSHDDADKQVRKALLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKITSNNRKLKE 239

Query: 244 -----IWGLDIKNLGELEKKGNGYPSWLKKGDRRLLGVLGREMEPNIVVAKDGSGNFKTV 303
                   +DI   GEL+ +  G+P+WL  GDRRLL   G  ++ +  VA DGSG FKTV
Sbjct: 240 ENQETTVAVDIAGAGELDSE--GWPTWLSAGDRRLL--QGSGVKADATVAADGSGTFKTV 299

Query: 304 KEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKKNVMIVGDGMDSTIITGSLNVVDGST 363
             AV + P+ S  R VI++K G Y ENVEV KKKKN+M +GDG   TIITGS NVVDGST
Sbjct: 300 AAAVAAAPENSNKRYVIHIKAGVYRENVEVAKKKKNIMFMGDGRTRTIITGSRNVVDGST 359

Query: 364 TFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVGADQSVINRCRIDAYQDTLYTHS 423
           TF SATVAAVG+ F+A+DI FQNTAGP KHQAVALRVG+D S    C + AYQDTLY HS
Sbjct: 360 TFHSATVAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHS 419

Query: 424 NRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQANMVTAQGRIDPNQNTGTSIQ 483
           NRQF+    I GTVDFIFGNAAVVLQ+C I  RRP + Q NMVTAQGR DPNQNTG  IQ
Sbjct: 420 NRQFFVKCLIAGTVDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQ 479

Query: 484 QCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVVMQSRIGDLIQPAGWAEWNGDFALKTLY 543
           +C I A+SDL+ VK S  TYLGRPWKEYS+TV+MQS I D+I+P GW+EW G FAL TL 
Sbjct: 480 KCRIGATSDLQSVKGSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLT 539

Query: 544 YGEYSNTGPGSDVSKRVKWAGYHAITSPSEAQKFTVNSLIQGGEWLGPSGATFIPGL 556
           Y EYSNTG G+  + RVKW G+  IT+ +EAQK+T    I GG WL  +G  F  GL
Sbjct: 540 YREYSNTGAGAGTANRVKWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL 592

BLAST of Chy2G026510 vs. TAIR 10
Match: AT5G53370.1 (pectin methylesterase PCR fragment F )

HSP 1 Score: 429.1 bits (1102), Expect = 5.4e-120
Identity = 246/567 (43.39%), Postives = 329/567 (58.02%), Query Frame = 0

Query: 12  PPKTSRSKTIPTILCFAAL----LCSAIF-----ITNKFIKPNPSENDLPFLQNICHKAY 71
           P    ++KT   +   A L    +C  IF     + +   +P  +      +   C K+ 
Sbjct: 24  PELQKKTKTKLILFTLAVLVVGVVCFGIFAGIRAVDSGKTEPKLTRKPTQAISRTCSKSL 83

Query: 72  DQSSCIEMAASEFPLSIIKTTNEVDFLQSFLRRSMPKVINTIERAKDLRQRINSPRGEAA 131
             + CI+    +FP S+  T +E + +      ++ K    +  +  +      PR  +A
Sbjct: 84  YPNLCID-TLLDFPGSL--TADENELIHISFNATLQKFSKALYTSSTITYTQMPPRVRSA 143

Query: 132 LADCIELMEISNG---RIMESVLALKNRTSGSIENSHTWLSSLLTNHVTC---WDEVESS 191
              C+EL++ S     R + SV+ +    S S  +  TWLSS +TNH TC   +DE+E  
Sbjct: 144 YDSCLELLDDSVDALTRALSSVVVVSGDESHS--DVMTWLSSAMTNHDTCTDGFDEIEGQ 203

Query: 192 LSRAAAMDLG-LEELIVRGRNSL----GMLVSIWGLDIKNLGEL--EKKGNGYPSWLKKG 251
                   +G +++L     N L    G +  + G+ + N  +L   ++    P+WLK+ 
Sbjct: 204 GGEVKDQVIGAVKDLSEMVSNCLAIFAGKVKDLSGVPVVNNRKLLGTEETEELPNWLKRE 263

Query: 252 DRRLLGVLGREMEPNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTY-EENVEV 311
           DR LLG     ++ +I V+KDGSG FKT+ EA++  P+ S  R VIYVK G Y EEN++V
Sbjct: 264 DRELLGTPTSAIQADITVSKDGSGTFKTIAEAIKKAPEHSSRRFVIYVKAGRYEEENLKV 323

Query: 312 GKKKKNVMIVGDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKH 371
           G+KK N+M +GDG   T+ITG  ++ D  TTF +AT AA G GFI +D+ F+N AGP KH
Sbjct: 324 GRKKTNLMFIGDGKGKTVITGGKSIADDLTTFHTATFAATGAGFIVRDMTFENYAGPAKH 383

Query: 372 QAVALRVGADQSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKI 431
           QAVALRVG D +V+ RC I  YQD LY HSNRQF+R+  I GTVDFIFGNAAV+LQ+C I
Sbjct: 384 QAVALRVGGDHAVVYRCNIIGYQDALYVHSNRQFFRECEIYGTVDFIFGNAAVILQSCNI 443

Query: 432 EPRRPMNNQANMVTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSR 491
             R+PM  Q   +TAQ R DPNQNTG SI  C ++A+ DLE  K S  TYLGRPWK YSR
Sbjct: 444 YARKPMAQQKITITAQNRKDPNQNTGISIHACKLLATPDLEASKGSYPTYLGRPWKLYSR 503

Query: 492 TVVMQSRIGDLIQPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKWAGYHAITSPSE 551
            V M S +GD I P GW EWNG FAL +LYYGEY N G GS + +RVKW GYH ITS  E
Sbjct: 504 VVYMMSDMGDHIDPRGWLEWNGPFALDSLYYGEYMNKGLGSGIGQRVKWPGYHVITSTVE 563

Query: 552 AQKFTVNSLIQGGEWLGPSGATFIPGL 556
           A KFTV   I G  WL  +G +F  GL
Sbjct: 564 ASKFTVAQFISGSSWLPSTGVSFFSGL 585

BLAST of Chy2G026510 vs. TAIR 10
Match: AT2G45220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 422.5 bits (1085), Expect = 5.1e-118
Identity = 230/474 (48.52%), Postives = 304/474 (64.14%), Query Frame = 0

Query: 84  NEVDFLQSFLRRSMPKVINTIERAKDLRQRINSPRGEAALADCIELMEISNGRIMESVLA 143
           +E +FL+  ++  + + I     A  L  +    R +AA  DCI+L +++  +I E+   
Sbjct: 58  SESEFLKISMKLVLDRAILAKTHAFTLGPKCRDTREKAAWEDCIKLYDLTVSKINET--- 117

Query: 144 LKNRTSGSIENSHTWLSSLLTNHVTCWDEVESSLSRAAAMDLGLEELI--VRGRNSLGML 203
           +      S  ++ TWLS+ LTN  TC         RA  ++LG+ +++  +   N   +L
Sbjct: 118 MDPNVKCSKLDAQTWLSTALTNLDTC---------RAGFLELGVTDIVLPLMSNNVSNLL 177

Query: 204 VSIWGLDIKNLGELEKKGNGYPSWLKKGDRRLLGVLGREMEPNIVVAKDGSGNFKTVKEA 263
            +   ++         + +G+PSW+K GDR+LL       + N VVAKDGSGNFKT+KEA
Sbjct: 178 CNTLAINKVPFNYTPPEKDGFPSWVKPGDRKLL--QSSTPKDNAVVAKDGSGNFKTIKEA 237

Query: 264 VESVPDKSKNRIVIYVKRGTYEENVEVGKKKKNVMIVGDGMDSTIITGSLNVVDGSTTFK 323
           +++       R VIYVK+G Y EN+E+  +KKNVM+ GDG+  TIITGS +V  G+TTF 
Sbjct: 238 IDAA--SGSGRFVIYVKQGVYSENLEI--RKKNVMLRGDGIGKTIITGSKSVGGGTTTFN 297

Query: 324 SATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVGADQSVINRCRIDAYQDTLYTHSNRQ 383
           SATVAAVGDGFIA+ I F+NTAG    QAVALR G+D SV  +C  +AYQDTLY HSNRQ
Sbjct: 298 SATVAAVGDGFIARGITFRNTAGASNEQAVALRSGSDLSVFYQCSFEAYQDTLYVHSNRQ 357

Query: 384 FYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQANMVTAQGRIDPNQNTGTSIQQCD 443
           FYRD  + GTVDFIFGNAA VLQNC I  RRP  ++ N +TAQGR DPNQNTG  I    
Sbjct: 358 FYRDCDVYGTVDFIFGNAAAVLQNCNIFARRP-RSKTNTITAQGRSDPNQNTGIIIHNSR 417

Query: 444 IVASSDLEPVKKSIKTYLGRPWKEYSRTVVMQSRIGDLIQPAGWAEWNGDFALKTLYYGE 503
           + A+SDL PV  S KTYLGRPW++YSRTV M++ +  LI P GW EW+G+FALKTL+Y E
Sbjct: 418 VTAASDLRPVLGSTKTYLGRPWRQYSRTVFMKTSLDSLIDPRGWLEWDGNFALKTLFYAE 477

Query: 504 YSNTGPGSDVSKRVKWAGYHAITSPSEAQKFTVNSLIQGGEWLGPSGATFIPGL 556
           + NTGPG+  S RV W G+  + S SEA KFTV + + GG W+ PS   F  GL
Sbjct: 478 FQNTGPGASTSGRVTWPGFRVLGSASEASKFTVGTFLAGGSWI-PSSVPFTSGL 511

BLAST of Chy2G026510 vs. TAIR 10
Match: AT1G53830.1 (pectin methylesterase 2 )

HSP 1 Score: 420.2 bits (1079), Expect = 2.5e-117
Identity = 244/529 (46.12%), Postives = 311/529 (58.79%), Query Frame = 0

Query: 54  LQNICHKAYDQSSCIEMAASEFPLSIIKTTNEVDFLQSFLRRSMPKVINTIERAKDL--R 113
           L+++C        C    A+     +   T++ + +++ L  +   V +     K L  +
Sbjct: 62  LKSVCSSTLYPELCFSAVAATGGKEL---TSQKEVIEASLNLTTKAVKHNYFAVKKLIAK 121

Query: 114 QRINSPRGEAALADCIELMEISNGRIMESVLALKNRTSGSIENSH-----TWLSSLLTNH 173
           ++  +PR   AL DC+E ++ +   +  +V  L           H     T +SS +TN 
Sbjct: 122 RKGLTPREVTALHDCLETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITNQ 181

Query: 174 VTC-----WDEVESSLSRAAAMDLGLEELIVRGRNSLGMLVSIWGLDIKNLGELEKK--- 233
            TC     +D+ +  + +A     G   +     N+L M+ ++   DI N    +K    
Sbjct: 182 GTCLDGFSYDDADRKVRKALLK--GQVHVEHMCSNALAMIKNMTETDIANFELRDKSSTF 241

Query: 234 ----------------GNGYPSWLKKGDRRLLGVLGREMEPNIVVAKDGSGNFKTVKEAV 293
                            +G+P WL  GDRRLL   G  ++ +  VA DGSG+F TV  AV
Sbjct: 242 TNNNNRKLKEVTGDLDSDGWPKWLSVGDRRLL--QGSTIKADATVADDGSGDFTTVAAAV 301

Query: 294 ESVPDKSKNRIVIYVKRGTYEENVEVGKKKKNVMIVGDGMDSTIITGSLNVVDGSTTFKS 353
            + P+KS  R VI++K G Y ENVEV KKK N+M +GDG   TIITGS NVVDGSTTF S
Sbjct: 302 AAAPEKSNKRFVIHIKAGVYRENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTFHS 361

Query: 354 ATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVGADQSVINRCRIDAYQDTLYTHSNRQF 413
           ATVAAVG+ F+A+DI FQNTAGP KHQAVALRVG+D S   +C + AYQDTLY HSNRQF
Sbjct: 362 ATVAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVHSNRQF 421

Query: 414 YRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQANMVTAQGRIDPNQNTGTSIQQCDI 473
           +    ITGTVDFIFGNAA VLQ+C I  RRP + Q NMVTAQGR DPNQNTG  IQ C I
Sbjct: 422 FVKCHITGTVDFIFGNAAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVIQNCRI 481

Query: 474 VASSDLEPVKKSIKTYLGRPWKEYSRTVVMQSRIGDLIQPAGWAEWNGDFALKTLYYGEY 533
             +SDL  VK +  TYLGRPWKEYSRTV+MQS I D+I+P GW EW+G FAL TL Y EY
Sbjct: 482 GGTSDLLAVKGTFPTYLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTLTYREY 541

Query: 534 SNTGPGSDVSKRVKWAGYHAITSPSEAQKFTVNSLIQGGEWLGPSGATF 552
            N G G+  + RVKW GY  ITS +EAQ FT    I GG WL  +G  F
Sbjct: 542 LNRGGGAGTANRVKWKGYKVITSDTEAQPFTAGQFIGGGGWLASTGFPF 583

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P839471.4e-16855.10Pectinesterase/pectinesterase inhibitor OS=Ficus pumila var. awkeotsang OX=20423... [more]
Q1JPL73.0e-16355.02Pectinesterase/pectinesterase inhibitor 18 OS=Arabidopsis thaliana OX=3702 GN=PM... [more]
Q965758.1e-16154.48Pectinesterase 2.2 OS=Solanum lycopersicum OX=4081 GN=PME2.2 PE=3 SV=1[more]
P096071.8e-16053.85Pectinesterase 2.1 OS=Solanum lycopersicum OX=4081 GN=PME2.1 PE=2 SV=2[more]
P142805.4e-15753.94Pectinesterase 1 OS=Solanum lycopersicum OX=4081 GN=PME1.9 PE=1 SV=5[more]
Match NameE-valueIdentityDescription
A0A0A0KHV50.0e+0097.48Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_6G514890 PE=3 SV=1[more]
A0A5D3CNY52.6e-30394.05Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G00664... [more]
A0A1S3B0187.5e-30394.05Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103484595 PE=3 SV=1[more]
A0A6J1KRW21.3e-25480.79Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111496691 PE=3 SV=1[more]
A0A6J1CN374.1e-24077.72Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111012508 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_004134752.10.097.48pectinesterase [Cucumis sativus] >KGN49128.1 hypothetical protein Csa_004290 [Cu... [more]
TYK13062.10.094.05pectinesterase [Cucumis melo var. makuwa][more]
XP_008439965.10.094.05PREDICTED: pectinesterase [Cucumis melo][more]
XP_038881670.10.089.21pectinesterase [Benincasa hispida][more]
XP_023002934.10.080.79pectinesterase-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT1G11580.12.1e-16455.02methylesterase PCR A [more]
AT3G14310.13.1e-12347.30pectin methylesterase 3 [more]
AT5G53370.15.4e-12043.39pectin methylesterase PCR fragment F [more]
AT2G45220.15.1e-11848.52Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT1G53830.12.5e-11746.12pectin methylesterase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 49..200
e-value: 9.9E-13
score: 58.3
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 53..179
e-value: 2.8E-14
score: 53.7
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 25..174
e-value: 2.8E-14
score: 50.8
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 244..540
e-value: 1.0E-152
score: 507.1
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 52..201
e-value: 2.7E-20
score: 74.8
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 53..175
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 238..555
e-value: 9.5E-126
score: 421.5
NoneNo IPR availablePANTHERPTHR31707:SF333PECTINESTERASEcoord: 14..554
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 14..554
NoneNo IPR availableCDDcd15799PMEI-like_4coord: 51..204
e-value: 2.48936E-28
score: 108.266
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 388..397
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 242..554

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy2G026510.1Chy2G026510.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0005576 extracellular region
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity