Chy2G023530 (gene) Cucumber (hystrix) v1

Overview
NameChy2G023530
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionHydrolase_4 domain-containing protein
LocationchrH02: 555228 .. 556214 (+)
RNA-Seq ExpressionChy2G023530
SyntenyChy2G023530
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCGCACCCAATAAGCCAAGCCAACGAGAACAGCCCCTATGGAGAGCTAACAAGGGAAGAATTCTACAAGAAACACAATATTACTCACCATGAAAGCTTCATCCTCAATGCACAGAAGATGAAGATCTTCACTCAATCTTGGCGGCCTGATTCTGACTCAAAGCTCAAAGGGGTTGTAGCCATGGTGCATGGCTACACCTGTGACAGTGGCTGGATATTCGAGCTCACTGGCGTCGCCATAGCCAAACTTGGGTTCCTCGTTTGCTCCCTCGACCTTCAAGGCCACGGTCGCTCCGAGGGAGCCCCTGGTTCAATCCCAGATATCGAACTCCTCGTCCTCGATTGTACACAGTTCTTCGACTCTATTCGAGAACAGCATCCAAACCTCCCCGCTTTTCTCTACGGAGAATCCCTAGGCGGTGCAATTTCAATCCTGATTTCCTTGAAGCAAGAGGGAGTTTGGAACGGAATAGTGTTAAACGGTTCCATGTGTGGAATCTCAGCGAAATTCAAACCCATTTGGCCATTGGAGAAGCTTCTTCCAATAGCAGCGTCTTTAGCGCCGTCATTACGGTTGGTGATATCGAAGCCCGTAGCAAGCAAATCATACAAAGAGGAATGGAAGAGAAGATTGGTAGCGAGAAACCCCAATCGGCGATTTTCAGGAAAACCACCAATGGCAACAGCATTGGAGTTTCTTCGAGTGTGCGAGTACATAAAGAGGAACTGCCATGAAATCAGAGTTCCAATGCTAATGGTTCATGGAGAAGACGACGTGGTTTGCGACAGCTGGTCGGCGAGATATGTGTACGAAGCGGCGGAGAGTGAAGACAAGACGTTGAAGGTATATCCCGGAATGTGGCATCAGTTGATCGGAGAGACGAAGGAGAATGTGGAGGTTGTTTATGCCACTATCTTCAACTGGTTAGTTGATAGAGCGGAGAAAGCCAAGAACAACAACCCCACCAAAAATAAGATAAAATAA

mRNA sequence

ATGTCGCACCCAATAAGCCAAGCCAACGAGAACAGCCCCTATGGAGAGCTAACAAGGGAAGAATTCTACAAGAAACACAATATTACTCACCATGAAAGCTTCATCCTCAATGCACAGAAGATGAAGATCTTCACTCAATCTTGGCGGCCTGATTCTGACTCAAAGCTCAAAGGGGTTGTAGCCATGGTGCATGGCTACACCTGTGACAGTGGCTGGATATTCGAGCTCACTGGCGTCGCCATAGCCAAACTTGGGTTCCTCGTTTGCTCCCTCGACCTTCAAGGCCACGGTCGCTCCGAGGGAGCCCCTGGTTCAATCCCAGATATCGAACTCCTCGTCCTCGATTGTACACAGTTCTTCGACTCTATTCGAGAACAGCATCCAAACCTCCCCGCTTTTCTCTACGGAGAATCCCTAGGCGGTGCAATTTCAATCCTGATTTCCTTGAAGCAAGAGGGAGTTTGGAACGGAATAGTGTTAAACGGTTCCATGTGTGGAATCTCAGCGAAATTCAAACCCATTTGGCCATTGGAGAAGCTTCTTCCAATAGCAGCGTCTTTAGCGCCGTCATTACGGTTGGTGATATCGAAGCCCGTAGCAAGCAAATCATACAAAGAGGAATGGAAGAGAAGATTGGTAGCGAGAAACCCCAATCGGCGATTTTCAGGAAAACCACCAATGGCAACAGCATTGGAGTTTCTTCGAGTGTGCGAGTACATAAAGAGGAACTGCCATGAAATCAGAGTTCCAATGCTAATGGTTCATGGAGAAGACGACGTGGTTTGCGACAGCTGGTCGGCGAGATATGTGTACGAAGCGGCGGAGAGTGAAGACAAGACGTTGAAGGTATATCCCGGAATGTGGCATCAGTTGATCGGAGAGACGAAGGAGAATGTGGAGGTTGTTTATGCCACTATCTTCAACTGGTTAGTTGATAGAGCGGAGAAAGCCAAGAACAACAACCCCACCAAAAATAAGATAAAATAA

Coding sequence (CDS)

ATGTCGCACCCAATAAGCCAAGCCAACGAGAACAGCCCCTATGGAGAGCTAACAAGGGAAGAATTCTACAAGAAACACAATATTACTCACCATGAAAGCTTCATCCTCAATGCACAGAAGATGAAGATCTTCACTCAATCTTGGCGGCCTGATTCTGACTCAAAGCTCAAAGGGGTTGTAGCCATGGTGCATGGCTACACCTGTGACAGTGGCTGGATATTCGAGCTCACTGGCGTCGCCATAGCCAAACTTGGGTTCCTCGTTTGCTCCCTCGACCTTCAAGGCCACGGTCGCTCCGAGGGAGCCCCTGGTTCAATCCCAGATATCGAACTCCTCGTCCTCGATTGTACACAGTTCTTCGACTCTATTCGAGAACAGCATCCAAACCTCCCCGCTTTTCTCTACGGAGAATCCCTAGGCGGTGCAATTTCAATCCTGATTTCCTTGAAGCAAGAGGGAGTTTGGAACGGAATAGTGTTAAACGGTTCCATGTGTGGAATCTCAGCGAAATTCAAACCCATTTGGCCATTGGAGAAGCTTCTTCCAATAGCAGCGTCTTTAGCGCCGTCATTACGGTTGGTGATATCGAAGCCCGTAGCAAGCAAATCATACAAAGAGGAATGGAAGAGAAGATTGGTAGCGAGAAACCCCAATCGGCGATTTTCAGGAAAACCACCAATGGCAACAGCATTGGAGTTTCTTCGAGTGTGCGAGTACATAAAGAGGAACTGCCATGAAATCAGAGTTCCAATGCTAATGGTTCATGGAGAAGACGACGTGGTTTGCGACAGCTGGTCGGCGAGATATGTGTACGAAGCGGCGGAGAGTGAAGACAAGACGTTGAAGGTATATCCCGGAATGTGGCATCAGTTGATCGGAGAGACGAAGGAGAATGTGGAGGTTGTTTATGCCACTATCTTCAACTGGTTAGTTGATAGAGCGGAGAAAGCCAAGAACAACAACCCCACCAAAAATAAGATAAAATAA

Protein sequence

MSHPISQANENSPYGELTREEFYKKHNITHHESFILNAQKMKIFTQSWRPDSDSKLKGVVAMVHGYTCDSGWIFELTGVAIAKLGFLVCSLDLQGHGRSEGAPGSIPDIELLVLDCTQFFDSIREQHPNLPAFLYGESLGGAISILISLKQEGVWNGIVLNGSMCGISAKFKPIWPLEKLLPIAASLAPSLRLVISKPVASKSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCEYIKRNCHEIRVPMLMVHGEDDVVCDSWSARYVYEAAESEDKTLKVYPGMWHQLIGETKENVEVVYATIFNWLVDRAEKAKNNNPTKNKIK*
Homology
BLAST of Chy2G023530 vs. ExPASy Swiss-Prot
Match: Q9C942 (Caffeoylshikimate esterase OS=Arabidopsis thaliana OX=3702 GN=CSE PE=1 SV=1)

HSP 1 Score: 186.0 bits (471), Expect = 6.7e-46
Identity = 101/307 (32.90%), Postives = 170/307 (55.37%), Query Frame = 0

Query: 14  YGELTREEFYKKHNITHHESFILNAQKMKIFTQSWRPDSDSKLKGVVAMVHGYTCDSGWI 73
           +G +  EE+Y    + + +S+       K+FTQS+ P  D ++KG V M HGY  D+ W+
Sbjct: 23  WGTMPEEEYYTSQGVRNSKSY-FETPNGKLFTQSFLP-LDGEIKGTVYMSHGYGSDTSWM 82

Query: 74  FELTGVAIAKLGFLVCSLDLQGHGRSEGAPGSIPDIELLVLDCTQFFDSIR--EQHPNLP 133
           F+   ++ +  G+ V + DL GHGRS+G    + D+E +      FF  +R  + + +LP
Sbjct: 83  FQKICMSFSSWGYAVFAADLLGHGRSDGIRCYMGDMEKVAATSLAFFKHVRCSDPYKDLP 142

Query: 134 AFLYGESLGGAISILISLKQE-GVWNGIVLNGSMCGISAKFKPIWPLEKLLPIAASLAPS 193
           AFL+GES+GG +++L+  + E   W G++ +  +  I    KP         +   LA +
Sbjct: 143 AFLFGESMGGLVTLLMYFQSEPETWTGLMFSAPLFVIPEDMKPSKAHLFAYGLLFGLADT 202

Query: 194 LRLVISKPVASKSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCEYIKRNCHEIRVP 253
              +    +  K+ K+  K +++A NP +R++GKP + T  E LR  +Y++ N  ++ +P
Sbjct: 203 WAAMPDNKMVGKAIKDPEKLKIIASNP-QRYTGKPRVGTMRELLRKTQYVQENFGKVTIP 262

Query: 254 MLMVHGEDDVVCDSWSARYVYEAAESEDKTLKVYPGMWHQLI-GETKENVEVVYATIFNW 313
           +   HG  D V    S++ +YE A S DKTLK+Y GM+H LI GE  EN E+V   +  W
Sbjct: 263 VFTAHGTADGVTCPTSSKLLYEKASSADKTLKIYEGMYHSLIQGEPDENAEIVLKDMREW 322

Query: 314 LVDRAEK 317
           + ++ +K
Sbjct: 323 IDEKVKK 326

BLAST of Chy2G023530 vs. ExPASy Swiss-Prot
Match: Q99685 (Monoglyceride lipase OS=Homo sapiens OX=9606 GN=MGLL PE=1 SV=2)

HSP 1 Score: 119.4 bits (298), Expect = 7.7e-26
Identity = 87/297 (29.29%), Postives = 138/297 (46.46%), Query Frame = 0

Query: 35  ILNAQKMKIFTQSWRPDSDSKLKGVVAMVHGYTCDSGWIFELTGVAIAKLGFLVCSLDLQ 94
           ++NA    +F + W+P      K ++ + HG    SG   EL  + +  L  LV + D  
Sbjct: 22  LVNADGQYLFCRYWKPTGTP--KALIFVSHGAGEHSGRYEELARMLMG-LDLLVFAHDHV 81

Query: 95  GHGRSEGAPGSIPDIELLVLDCTQFFDSIREQHPNLPAFLYGESLGGAISILISLKQEGV 154
           GHG+SEG    + D  + V D  Q  DS+++ +P LP FL G S+GGAI+IL + ++ G 
Sbjct: 82  GHGQSEGERMVVSDFHVFVRDVLQHVDSMQKDYPGLPVFLLGHSMGGAIAILTAAERPGH 141

Query: 155 WNGIVLNGSMCGISAKFKPIWPLEKLLPIAASLAPSLRLVISKPVASKSYKEEWKRRLVA 214
           + G+VL             I PL    P +A+    L   +   V            +++
Sbjct: 142 FAGMVL-------------ISPLVLANPESATTFKVLAAKVLNLVLPNLSLGPIDSSVLS 201

Query: 215 RNPNR--RFSGKPPMATA-------LEFLRVCEYIKRNCHEIRVPMLMVHGEDDVVCDSW 274
           RN      ++  P +  A       ++ L     ++R   ++ VP L++ G  D +CDS 
Sbjct: 202 RNKTEVDIYNSDPLICRAGLKVCFGIQLLNAVSRVERALPKLTVPFLLLQGSADRLCDSK 261

Query: 275 SARYVYEAAESEDKTLKVYPGMWHQLIGETKENVEVVYATIFNWLVDRAEKAKNNNP 323
            A  + E A+S+DKTLK+Y G +H L  E  E    V+  I  W+  R   A   +P
Sbjct: 262 GAYLLMELAKSQDKTLKIYEGAYHVLHKELPEVTNSVFHEINMWVSQRTATAGTASP 302

BLAST of Chy2G023530 vs. ExPASy Swiss-Prot
Match: O35678 (Monoglyceride lipase OS=Mus musculus OX=10090 GN=Mgll PE=1 SV=1)

HSP 1 Score: 115.2 bits (287), Expect = 1.4e-24
Identity = 82/292 (28.08%), Postives = 138/292 (47.26%), Query Frame = 0

Query: 35  ILNAQKMKIFTQSWRPDSDSKLKGVVAMVHGYTCDSGWIFELTGVAIAKLGFLVCSLDLQ 94
           ++NA    +F + W+P      K ++ + HG     G   EL  + +  L  LV + D  
Sbjct: 22  LVNADGQYLFCRYWKPSGTP--KALIFVSHGAGEHCGRYDELAHM-LKGLDMLVFAHDHV 81

Query: 95  GHGRSEGAPGSIPDIELLVLDCTQFFDSIREQHPNLPAFLYGESLGGAISILISLKQEGV 154
           GHG+SEG    + D ++ V D  Q  D+I++ +P++P FL G S+GGAISIL++ ++   
Sbjct: 82  GHGQSEGERMVVSDFQVFVRDVLQHVDTIQKDYPDVPIFLLGHSMGGAISILVAAERPTY 141

Query: 155 WNGIVLNGSMCGISAKFKPIWPLEKLLPIAASLAPSLRLVISKPVASKSYKEEWKRRLVA 214
           ++G+VL             I PL    P +AS    L   +   V            +++
Sbjct: 142 FSGMVL-------------ISPLVLANPESASTLKVLAAKLLNFVLPNMTLGRIDSSVLS 201

Query: 215 RNPNR--RFSGKPPMATA-------LEFLRVCEYIKRNCHEIRVPMLMVHGEDDVVCDSW 274
           RN +    ++  P +  A       ++ L     ++R    + +P L++ G  D +CDS 
Sbjct: 202 RNKSEVDLYNSDPLVCRAGLKVCFGIQLLNAVARVERAMPRLTLPFLLLQGSADRLCDSK 261

Query: 275 SARYVYEAAESEDKTLKVYPGMWHQLIGETKENVEVVYATIFNWLVDRAEKA 318
            A  + E++ S+DKTLK+Y G +H L  E  E    V   + +W+  R   A
Sbjct: 262 GAYLLMESSRSQDKTLKMYEGAYHVLHRELPEVTNSVLHEVNSWVSHRIAAA 297

BLAST of Chy2G023530 vs. ExPASy Swiss-Prot
Match: Q8R431 (Monoglyceride lipase OS=Rattus norvegicus OX=10116 GN=Mgll PE=1 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 6.1e-23
Identity = 79/288 (27.43%), Postives = 135/288 (46.88%), Query Frame = 0

Query: 35  ILNAQKMKIFTQSWRPDSDSKLKGVVAMVHGYTCDSGWIFELTGVAIAKLGFLVCSLDLQ 94
           ++NA    +F + W+P      K ++ + HG     G   EL  + + +L  LV + D  
Sbjct: 22  LVNADGQYLFCRYWKPSGTP--KALIFVSHGAGEHCGRYDELAQM-LKRLDMLVFAHDHV 81

Query: 95  GHGRSEGAPGSIPDIELLVLDCTQFFDSIREQHPNLPAFLYGESLGGAISILISLKQEGV 154
           GHG+SEG    + D ++ V D  Q  +++++ +P +P FL G S+GGAISIL + ++   
Sbjct: 82  GHGQSEGERMVVSDFQVFVRDLLQHVNTVQKDYPEVPVFLLGHSMGGAISILAAAERPTH 141

Query: 155 WNGIVLNGSMCGISAKFKPIWPLEKLLPIAASLAPSLRLVISKPVASKSYKEEWKRRLVA 214
           ++G++L             I PL    P +AS    L   +   V            +++
Sbjct: 142 FSGMIL-------------ISPLILANPESASTLKVLAAKLLNFVLPNISLGRIDSSVLS 201

Query: 215 RNPNR--RFSGKPPMATA-------LEFLRVCEYIKRNCHEIRVPMLMVHGEDDVVCDSW 274
           RN +    ++  P +  A       ++ L     ++R    + +P L++ G  D +CDS 
Sbjct: 202 RNKSEVDLYNSDPLICHAGVKVCFGIQLLNAVSRVERAMPRLTLPFLLLQGSADRLCDSK 261

Query: 275 SARYVYEAAESEDKTLKVYPGMWHQLIGETKENVEVVYATIFNWLVDR 314
            A  + E++ S+DKTLK+Y G +H L  E  E    V   I  W+  R
Sbjct: 262 GAYLLMESSPSQDKTLKMYEGAYHVLHKELPEVTNSVLHEINTWVSHR 293

BLAST of Chy2G023530 vs. ExPASy Swiss-Prot
Match: A0QNZ7 (Monoacylglycerol lipase OS=Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) OX=246196 GN=MSMEG_0220 PE=1 SV=1)

HSP 1 Score: 109.0 bits (271), Expect = 1.0e-22
Identity = 75/290 (25.86%), Postives = 124/290 (42.76%), Query Frame = 0

Query: 29  THHESFILNAQKMKIFTQSWRPDSDSKLKGVVAMVHGYTCDSGWIFELTGVAIAKLGFLV 88
           T  E        ++I    W PD+D   +GVV + HGY   +G  +          G LV
Sbjct: 5   TRSEHSFAGVGGVRIVYDVWTPDTDP--RGVVVLAHGYAEHAG-RYHHVAQRFGAAGLLV 64

Query: 89  CSLDLQGHGRSEGAPGSIPDIELLVLDCTQFFDSIREQHPNLPAFLYGESLGGAISILIS 148
            +LD +GHGRS G    + D+   V D           HP LP  + G S+GG I     
Sbjct: 65  YALDHRGHGRSGGKRVHLRDLSEFVEDFRTLVGIAANDHPTLPRIVLGHSMGGGIVFAYG 124

Query: 149 LKQEGVWNGIVLNGSMCGISAKFKPIWPLEKLLPIAASLAPSLRL-------VISKPVAS 208
            +  G ++ +VL+G          P+  L  +  +   LAP + +       V   P   
Sbjct: 125 ARYPGEYSAMVLSGPAVNAHDGVSPV--LVAVAKVLGKLAPGIPVENLDADAVSRDPEVV 184

Query: 209 KSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCEYIKRNCHEIRVPMLMVHGEDDVV 268
            +YK +               GK P   A   + + + + +    +  P+L+VHG+ D +
Sbjct: 185 AAYKAD----------PMVHHGKLPAGIARALIGLGQSMPQRAAALTAPLLVVHGDKDRL 244

Query: 269 CDSWSARYVYEAAESEDKTLKVYPGMWHQLIGETKENVEVVYATIFNWLV 312
                +R + +   SED  LKVYPG++H++  E ++  ++V   + +W+V
Sbjct: 245 IPVAGSRLLVDRVASEDVHLKVYPGLYHEVFNEPEQ--KLVLDDVTSWIV 277

BLAST of Chy2G023530 vs. ExPASy TrEMBL
Match: A0A0A0KKV9 (Hydrolase_4 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G525500 PE=4 SV=1)

HSP 1 Score: 659.1 bits (1699), Expect = 9.9e-186
Identity = 317/328 (96.65%), Postives = 321/328 (97.87%), Query Frame = 0

Query: 1   MSHPISQANENSPYGELTREEFYKKHNITHHESFILNAQKMKIFTQSWRPDSDSKLKGVV 60
           MSHPISQANENSPYGELTREEFYKKHN+THHESFILNAQKMKIFTQSW+PDSDSKLKGVV
Sbjct: 1   MSHPISQANENSPYGELTREEFYKKHNVTHHESFILNAQKMKIFTQSWQPDSDSKLKGVV 60

Query: 61  AMVHGYTCDSGWIFELTGVAIAKLGFLVCSLDLQGHGRSEGAPGSIPDIELLVLDCTQFF 120
           AMVHGYTCDSGWIFELTG+AIAKLGFLVCSLDLQGHGRSEGAPGSI DIELLVLDCTQFF
Sbjct: 61  AMVHGYTCDSGWIFELTGIAIAKLGFLVCSLDLQGHGRSEGAPGSIRDIELLVLDCTQFF 120

Query: 121 DSIREQHPNLPAFLYGESLGGAISILISLKQEGVWNGIVLNGSMCGISAKFKPIWPLEKL 180
           DSIREQHPNLPAFLYGESLGGAISILISLKQEGVWNGIVLNGSMCGISAKFKPIWPLEKL
Sbjct: 121 DSIREQHPNLPAFLYGESLGGAISILISLKQEGVWNGIVLNGSMCGISAKFKPIWPLEKL 180

Query: 181 LPIAASLAPSLRLVISKPVASKSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCEYI 240
           LPIAASLAPSLRLVISKPVASKSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCEYI
Sbjct: 181 LPIAASLAPSLRLVISKPVASKSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCEYI 240

Query: 241 KRNCHEIRVPMLMVHGEDDVVCDSWSARYVYEAAESEDKTLKVYPGMWHQLIGETKENVE 300
           KRNCHEIRVP+LMVHGEDDVVCDSWSARYVYEAAESEDKTLKVYPGMWHQLIGETKENVE
Sbjct: 241 KRNCHEIRVPLLMVHGEDDVVCDSWSARYVYEAAESEDKTLKVYPGMWHQLIGETKENVE 300

Query: 301 VVYATIFNWLVDRAEKAKNNNPTKNKIK 329
           VVY TIFNWLVDRAEKA N   TKN IK
Sbjct: 301 VVYGTIFNWLVDRAEKADNTTTTKNNIK 328

BLAST of Chy2G023530 vs. ExPASy TrEMBL
Match: A0A5A7UE91 (Caffeoylshikimate esterase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G009620 PE=4 SV=1)

HSP 1 Score: 644.0 bits (1660), Expect = 3.3e-181
Identity = 310/330 (93.94%), Postives = 319/330 (96.67%), Query Frame = 0

Query: 1   MSHPISQANENSPYGELTREEFYKKHNITHHESFILNAQKMKIFTQSWRPDSDSKLKGVV 60
           MSHPI+QANENSPYGELTREEFYKKHN+THHESFILNAQKMKIFTQSWRPDSD+KLKGVV
Sbjct: 1   MSHPINQANENSPYGELTREEFYKKHNVTHHESFILNAQKMKIFTQSWRPDSDTKLKGVV 60

Query: 61  AMVHGYTCDSGWIFELTGVAIAKLGFLVCSLDLQGHGRSEGAPGSIPDIELLVLDCTQFF 120
           AMVHGYTCDSGWIFELTGVAIAKLGFLVCSLDLQGHGRSEGAPGSIPDIELLV DCT FF
Sbjct: 61  AMVHGYTCDSGWIFELTGVAIAKLGFLVCSLDLQGHGRSEGAPGSIPDIELLVHDCTLFF 120

Query: 121 DSIREQHPNLPAFLYGESLGGAISILISLKQEGVWNGIVLNGSMCGISAKFKPIWPLEKL 180
           DSIREQHPNLPAFLYGESLGGAISILISLKQEGVWNGIVLNGSMCGIS KFKPIWPLEKL
Sbjct: 121 DSIREQHPNLPAFLYGESLGGAISILISLKQEGVWNGIVLNGSMCGISGKFKPIWPLEKL 180

Query: 181 LPIAASLAPSLRLVISKPVASKSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCEYI 240
           LPIAASLAPSLRLVISKPVASKSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCEYI
Sbjct: 181 LPIAASLAPSLRLVISKPVASKSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCEYI 240

Query: 241 KRNCHEIRVPMLMVHGEDDVVCDSWSARYVYEAAESEDKTLKVYPGMWHQLIGETKENVE 300
           KRNCHEIRVPMLMVHGEDDVVCDSWSARYV+EAAESEDKTLKVYPGMWHQLIGE KE+VE
Sbjct: 241 KRNCHEIRVPMLMVHGEDDVVCDSWSARYVFEAAESEDKTLKVYPGMWHQLIGEAKEDVE 300

Query: 301 VVYATIFNWLVDRAEKA--KNNNPTKNKIK 329
           +VY TI NWLVD+A+KA   NNN  K+KIK
Sbjct: 301 IVYGTILNWLVDKADKANNSNNNAAKDKIK 330

BLAST of Chy2G023530 vs. ExPASy TrEMBL
Match: A0A1S3B390 (caffeoylshikimate esterase-like OS=Cucumis melo OX=3656 GN=LOC103485302 PE=4 SV=1)

HSP 1 Score: 641.7 bits (1654), Expect = 1.6e-180
Identity = 309/330 (93.64%), Postives = 318/330 (96.36%), Query Frame = 0

Query: 1   MSHPISQANENSPYGELTREEFYKKHNITHHESFILNAQKMKIFTQSWRPDSDSKLKGVV 60
           MSHPI+QANENSPYGELTREEFYKKHN+THHESFILNAQKMKIFTQSWRPDSD+KLKGVV
Sbjct: 1   MSHPINQANENSPYGELTREEFYKKHNVTHHESFILNAQKMKIFTQSWRPDSDTKLKGVV 60

Query: 61  AMVHGYTCDSGWIFELTGVAIAKLGFLVCSLDLQGHGRSEGAPGSIPDIELLVLDCTQFF 120
           AMVHGYTCDSGWIFELTGVAIAKLGFLVCSLDLQGHGRSEGAPGSIPDIELLV DCT FF
Sbjct: 61  AMVHGYTCDSGWIFELTGVAIAKLGFLVCSLDLQGHGRSEGAPGSIPDIELLVHDCTLFF 120

Query: 121 DSIREQHPNLPAFLYGESLGGAISILISLKQEGVWNGIVLNGSMCGISAKFKPIWPLEKL 180
           DSIREQHPNLPAFLYGESLGGAISILISLKQEGVWNGIVLNG MCGIS KFKPIWPLEKL
Sbjct: 121 DSIREQHPNLPAFLYGESLGGAISILISLKQEGVWNGIVLNGFMCGISGKFKPIWPLEKL 180

Query: 181 LPIAASLAPSLRLVISKPVASKSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCEYI 240
           LPIAASLAPSLRLVISKPVASKSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCEYI
Sbjct: 181 LPIAASLAPSLRLVISKPVASKSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCEYI 240

Query: 241 KRNCHEIRVPMLMVHGEDDVVCDSWSARYVYEAAESEDKTLKVYPGMWHQLIGETKENVE 300
           KRNCHEIRVPMLMVHGEDDVVCDSWSARYV+EAAESEDKTLKVYPGMWHQLIGE KE+VE
Sbjct: 241 KRNCHEIRVPMLMVHGEDDVVCDSWSARYVFEAAESEDKTLKVYPGMWHQLIGEAKEDVE 300

Query: 301 VVYATIFNWLVDRAEKA--KNNNPTKNKIK 329
           +VY TI NWLVD+A+KA   NNN  K+KIK
Sbjct: 301 IVYGTILNWLVDKADKANNSNNNAAKDKIK 330

BLAST of Chy2G023530 vs. ExPASy TrEMBL
Match: A0A6J1ECA8 (caffeoylshikimate esterase-like OS=Cucurbita moschata OX=3662 GN=LOC111433016 PE=4 SV=1)

HSP 1 Score: 604.0 bits (1556), Expect = 3.8e-169
Identity = 290/328 (88.41%), Postives = 308/328 (93.90%), Query Frame = 0

Query: 1   MSHPISQANENSPYGELTREEFYKKHNITHHESFILNAQKMKIFTQSWRPDSDSKLKGVV 60
           M+HPI+QANENSPYG LTREEFYKKHN+THHE+FILNAQKMKIFTQSWRP SDS+LKGVV
Sbjct: 1   MAHPINQANENSPYGGLTREEFYKKHNVTHHETFILNAQKMKIFTQSWRPASDSELKGVV 60

Query: 61  AMVHGYTCDSGWIFELTGVAIAKLGFLVCSLDLQGHGRSEGAPGSIPDIELLVLDCTQFF 120
           AMVHGYT DSGW+FELT VAIAK GFLVCSLDLQGHGRS+GAPGSIPDIELLVLDCT FF
Sbjct: 61  AMVHGYTSDSGWMFELTAVAIAKSGFLVCSLDLQGHGRSDGAPGSIPDIELLVLDCTLFF 120

Query: 121 DSIREQHPNLPAFLYGESLGGAISILISLKQEGVWNGIVLNGSMCGISAKFKPIWPLEKL 180
           DS+REQ+P LPAFLYGESLGGAISILI LKQEGVW+GIVLNGSMCGISAKF+PIWPLEKL
Sbjct: 121 DSVREQNPTLPAFLYGESLGGAISILICLKQEGVWSGIVLNGSMCGISAKFRPIWPLEKL 180

Query: 181 LPIAASLAPSLRLVISKPVASKSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCEYI 240
           LPIAASLAP LRLV+SKP ASKSYKEEWKRRLVA+NPNRRFSGKPPMATALEFLRVCEYI
Sbjct: 181 LPIAASLAPWLRLVVSKPHASKSYKEEWKRRLVAKNPNRRFSGKPPMATALEFLRVCEYI 240

Query: 241 KRNCHEIRVPMLMVHGEDDVVCDSWSARYVYEAAESEDKTLKVYPGMWHQLIGETKENVE 300
           K NCHEIRVPMLMVHGEDDVVCDS SAR+V+E+A SEDKTLKVYPGMWHQLIGE  ENVE
Sbjct: 241 KMNCHEIRVPMLMVHGEDDVVCDSESARFVFESAASEDKTLKVYPGMWHQLIGEANENVE 300

Query: 301 VVYATIFNWLVDRAEKAKNNNPTKNKIK 329
           +VYATIFNWLVDRAEKAK  N +KN  K
Sbjct: 301 IVYATIFNWLVDRAEKAKAKNKSKNTKK 328

BLAST of Chy2G023530 vs. ExPASy TrEMBL
Match: A0A6J1IPF3 (caffeoylshikimate esterase-like OS=Cucurbita maxima OX=3661 GN=LOC111477621 PE=4 SV=1)

HSP 1 Score: 597.0 bits (1538), Expect = 4.6e-167
Identity = 286/326 (87.73%), Postives = 305/326 (93.56%), Query Frame = 0

Query: 1   MSHPISQANENSPYGELTREEFYKKHNITHHESFILNAQKMKIFTQSWRPDSDSKLKGVV 60
           M+HPI+QANENSPYG LTRE+FY+KHN+TH E+FILNAQKMKIFTQSWRP SDS+LKGVV
Sbjct: 1   MAHPINQANENSPYGGLTREDFYQKHNVTHQETFILNAQKMKIFTQSWRPVSDSELKGVV 60

Query: 61  AMVHGYTCDSGWIFELTGVAIAKLGFLVCSLDLQGHGRSEGAPGSIPDIELLVLDCTQFF 120
           AMVHGYT DSGWIFELT VA+AK GFLVCSLDLQGHGRS+GAPGSIPDIELLVLDCT FF
Sbjct: 61  AMVHGYTSDSGWIFELTAVALAKSGFLVCSLDLQGHGRSDGAPGSIPDIELLVLDCTLFF 120

Query: 121 DSIREQHPNLPAFLYGESLGGAISILISLKQEGVWNGIVLNGSMCGISAKFKPIWPLEKL 180
           DS+REQ+P LPAFLYGESLGGAISILI LKQEGVW+GIVLNGSMCGISAKF+PIWPLEKL
Sbjct: 121 DSVREQNPTLPAFLYGESLGGAISILICLKQEGVWSGIVLNGSMCGISAKFRPIWPLEKL 180

Query: 181 LPIAASLAPSLRLVISKPVASKSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCEYI 240
           LPIAASLAPSLRLV SKP ASKSYKEEWKRRLVA+NPNRRFSGKPPMATALEFLRVC+YI
Sbjct: 181 LPIAASLAPSLRLVASKPHASKSYKEEWKRRLVAKNPNRRFSGKPPMATALEFLRVCKYI 240

Query: 241 KRNCHEIRVPMLMVHGEDDVVCDSWSARYVYEAAESEDKTLKVYPGMWHQLIGETKENVE 300
           K NCHEIRVPMLMVHGEDDVVCDS SAR+V+E+A SEDKTLKVYPGMWHQLIGE  ENVE
Sbjct: 241 KMNCHEIRVPMLMVHGEDDVVCDSESARFVFESAASEDKTLKVYPGMWHQLIGEANENVE 300

Query: 301 VVYATIFNWLVDRAEKAKNNNPTKNK 327
           +VYATIFNWLVDRAEKAKN N    K
Sbjct: 301 IVYATIFNWLVDRAEKAKNKNKDTKK 326

BLAST of Chy2G023530 vs. NCBI nr
Match: KGN49464.1 (hypothetical protein Csa_003642 [Cucumis sativus])

HSP 1 Score: 657 bits (1696), Expect = 3.84e-238
Identity = 317/328 (96.65%), Postives = 321/328 (97.87%), Query Frame = 0

Query: 1   MSHPISQANENSPYGELTREEFYKKHNITHHESFILNAQKMKIFTQSWRPDSDSKLKGVV 60
           MSHPISQANENSPYGELTREEFYKKHN+THHESFILNAQKMKIFTQSW+PDSDSKLKGVV
Sbjct: 1   MSHPISQANENSPYGELTREEFYKKHNVTHHESFILNAQKMKIFTQSWQPDSDSKLKGVV 60

Query: 61  AMVHGYTCDSGWIFELTGVAIAKLGFLVCSLDLQGHGRSEGAPGSIPDIELLVLDCTQFF 120
           AMVHGYTCDSGWIFELTG+AIAKLGFLVCSLDLQGHGRSEGAPGSI DIELLVLDCTQFF
Sbjct: 61  AMVHGYTCDSGWIFELTGIAIAKLGFLVCSLDLQGHGRSEGAPGSIRDIELLVLDCTQFF 120

Query: 121 DSIREQHPNLPAFLYGESLGGAISILISLKQEGVWNGIVLNGSMCGISAKFKPIWPLEKL 180
           DSIREQHPNLPAFLYGESLGGAISILISLKQEGVWNGIVLNGSMCGISAKFKPIWPLEKL
Sbjct: 121 DSIREQHPNLPAFLYGESLGGAISILISLKQEGVWNGIVLNGSMCGISAKFKPIWPLEKL 180

Query: 181 LPIAASLAPSLRLVISKPVASKSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCEYI 240
           LPIAASLAPSLRLVISKPVASKSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCEYI
Sbjct: 181 LPIAASLAPSLRLVISKPVASKSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCEYI 240

Query: 241 KRNCHEIRVPMLMVHGEDDVVCDSWSARYVYEAAESEDKTLKVYPGMWHQLIGETKENVE 300
           KRNCHEIRVP+LMVHGEDDVVCDSWSARYVYEAAESEDKTLKVYPGMWHQLIGETKENVE
Sbjct: 241 KRNCHEIRVPLLMVHGEDDVVCDSWSARYVYEAAESEDKTLKVYPGMWHQLIGETKENVE 300

Query: 301 VVYATIFNWLVDRAEKAKNNNPTKNKIK 328
           VVY TIFNWLVDRAEKA N   TKN IK
Sbjct: 301 VVYGTIFNWLVDRAEKADNTTTTKNNIK 328

BLAST of Chy2G023530 vs. NCBI nr
Match: KAA0052486.1 (caffeoylshikimate esterase-like [Cucumis melo var. makuwa] >TYK13337.1 caffeoylshikimate esterase-like [Cucumis melo var. makuwa])

HSP 1 Score: 642 bits (1657), Expect = 3.65e-232
Identity = 310/330 (93.94%), Postives = 319/330 (96.67%), Query Frame = 0

Query: 1   MSHPISQANENSPYGELTREEFYKKHNITHHESFILNAQKMKIFTQSWRPDSDSKLKGVV 60
           MSHPI+QANENSPYGELTREEFYKKHN+THHESFILNAQKMKIFTQSWRPDSD+KLKGVV
Sbjct: 1   MSHPINQANENSPYGELTREEFYKKHNVTHHESFILNAQKMKIFTQSWRPDSDTKLKGVV 60

Query: 61  AMVHGYTCDSGWIFELTGVAIAKLGFLVCSLDLQGHGRSEGAPGSIPDIELLVLDCTQFF 120
           AMVHGYTCDSGWIFELTGVAIAKLGFLVCSLDLQGHGRSEGAPGSIPDIELLV DCT FF
Sbjct: 61  AMVHGYTCDSGWIFELTGVAIAKLGFLVCSLDLQGHGRSEGAPGSIPDIELLVHDCTLFF 120

Query: 121 DSIREQHPNLPAFLYGESLGGAISILISLKQEGVWNGIVLNGSMCGISAKFKPIWPLEKL 180
           DSIREQHPNLPAFLYGESLGGAISILISLKQEGVWNGIVLNGSMCGIS KFKPIWPLEKL
Sbjct: 121 DSIREQHPNLPAFLYGESLGGAISILISLKQEGVWNGIVLNGSMCGISGKFKPIWPLEKL 180

Query: 181 LPIAASLAPSLRLVISKPVASKSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCEYI 240
           LPIAASLAPSLRLVISKPVASKSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCEYI
Sbjct: 181 LPIAASLAPSLRLVISKPVASKSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCEYI 240

Query: 241 KRNCHEIRVPMLMVHGEDDVVCDSWSARYVYEAAESEDKTLKVYPGMWHQLIGETKENVE 300
           KRNCHEIRVPMLMVHGEDDVVCDSWSARYV+EAAESEDKTLKVYPGMWHQLIGE KE+VE
Sbjct: 241 KRNCHEIRVPMLMVHGEDDVVCDSWSARYVFEAAESEDKTLKVYPGMWHQLIGEAKEDVE 300

Query: 301 VVYATIFNWLVDRAEKAKN--NNPTKNKIK 328
           +VY TI NWLVD+A+KA N  NN  K+KIK
Sbjct: 301 IVYGTILNWLVDKADKANNSNNNAAKDKIK 330

BLAST of Chy2G023530 vs. NCBI nr
Match: XP_008441075.1 (PREDICTED: caffeoylshikimate esterase-like [Cucumis melo])

HSP 1 Score: 640 bits (1651), Expect = 3.00e-231
Identity = 309/330 (93.64%), Postives = 318/330 (96.36%), Query Frame = 0

Query: 1   MSHPISQANENSPYGELTREEFYKKHNITHHESFILNAQKMKIFTQSWRPDSDSKLKGVV 60
           MSHPI+QANENSPYGELTREEFYKKHN+THHESFILNAQKMKIFTQSWRPDSD+KLKGVV
Sbjct: 1   MSHPINQANENSPYGELTREEFYKKHNVTHHESFILNAQKMKIFTQSWRPDSDTKLKGVV 60

Query: 61  AMVHGYTCDSGWIFELTGVAIAKLGFLVCSLDLQGHGRSEGAPGSIPDIELLVLDCTQFF 120
           AMVHGYTCDSGWIFELTGVAIAKLGFLVCSLDLQGHGRSEGAPGSIPDIELLV DCT FF
Sbjct: 61  AMVHGYTCDSGWIFELTGVAIAKLGFLVCSLDLQGHGRSEGAPGSIPDIELLVHDCTLFF 120

Query: 121 DSIREQHPNLPAFLYGESLGGAISILISLKQEGVWNGIVLNGSMCGISAKFKPIWPLEKL 180
           DSIREQHPNLPAFLYGESLGGAISILISLKQEGVWNGIVLNG MCGIS KFKPIWPLEKL
Sbjct: 121 DSIREQHPNLPAFLYGESLGGAISILISLKQEGVWNGIVLNGFMCGISGKFKPIWPLEKL 180

Query: 181 LPIAASLAPSLRLVISKPVASKSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCEYI 240
           LPIAASLAPSLRLVISKPVASKSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCEYI
Sbjct: 181 LPIAASLAPSLRLVISKPVASKSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCEYI 240

Query: 241 KRNCHEIRVPMLMVHGEDDVVCDSWSARYVYEAAESEDKTLKVYPGMWHQLIGETKENVE 300
           KRNCHEIRVPMLMVHGEDDVVCDSWSARYV+EAAESEDKTLKVYPGMWHQLIGE KE+VE
Sbjct: 241 KRNCHEIRVPMLMVHGEDDVVCDSWSARYVFEAAESEDKTLKVYPGMWHQLIGEAKEDVE 300

Query: 301 VVYATIFNWLVDRAEKAKN--NNPTKNKIK 328
           +VY TI NWLVD+A+KA N  NN  K+KIK
Sbjct: 301 IVYGTILNWLVDKADKANNSNNNAAKDKIK 330

BLAST of Chy2G023530 vs. NCBI nr
Match: XP_038881607.1 (caffeoylshikimate esterase-like [Benincasa hispida])

HSP 1 Score: 621 bits (1602), Expect = 7.33e-224
Identity = 298/320 (93.12%), Postives = 309/320 (96.56%), Query Frame = 0

Query: 1   MSHPISQANENSPYGELTREEFYKKHNITHHESFILNAQKMKIFTQSWRPDSDSKLKGVV 60
           M HPI+QANENSPYG LTREEFYKKH +THHESFILNAQKMKIFTQSWRPDSDS+LKGVV
Sbjct: 1   MLHPINQANENSPYGGLTREEFYKKHKVTHHESFILNAQKMKIFTQSWRPDSDSELKGVV 60

Query: 61  AMVHGYTCDSGWIFELTGVAIAKLGFLVCSLDLQGHGRSEGAPGSIPDIELLVLDCTQFF 120
           AMVHGYT DSGWIFELT VAIAKLGFLVCSLDLQGHGRSEGAPGSIPDIELLVLDCT FF
Sbjct: 61  AMVHGYTSDSGWIFELTAVAIAKLGFLVCSLDLQGHGRSEGAPGSIPDIELLVLDCTLFF 120

Query: 121 DSIREQHPNLPAFLYGESLGGAISILISLKQEGVWNGIVLNGSMCGISAKFKPIWPLEKL 180
           DS+REQ+PNLPAFLYGESLGGAISILISLKQ+GVWNGIVLNGSMCGISAKFKPIWPLEKL
Sbjct: 121 DSVREQNPNLPAFLYGESLGGAISILISLKQKGVWNGIVLNGSMCGISAKFKPIWPLEKL 180

Query: 181 LPIAASLAPSLRLVISKPVASKSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCEYI 240
           LPIAASLAPSLRLVI+KPVASKSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCEYI
Sbjct: 181 LPIAASLAPSLRLVITKPVASKSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCEYI 240

Query: 241 KRNCHEIRVPMLMVHGEDDVVCDSWSARYVYEAAESEDKTLKVYPGMWHQLIGETKENVE 300
           KRNCHEIRVPMLMVHGEDDVVCDSWSAR+V+E+AESEDKTLKVYPGMWHQLIGE KE+VE
Sbjct: 241 KRNCHEIRVPMLMVHGEDDVVCDSWSARFVFESAESEDKTLKVYPGMWHQLIGEPKEDVE 300

Query: 301 VVYATIFNWLVDRAEKAKNN 320
           VVY TIF WLVDRAEK  NN
Sbjct: 301 VVYGTIFTWLVDRAEKTNNN 320

BLAST of Chy2G023530 vs. NCBI nr
Match: XP_022925667.1 (caffeoylshikimate esterase-like [Cucurbita moschata])

HSP 1 Score: 602 bits (1553), Expect = 2.41e-216
Identity = 289/325 (88.92%), Postives = 307/325 (94.46%), Query Frame = 0

Query: 1   MSHPISQANENSPYGELTREEFYKKHNITHHESFILNAQKMKIFTQSWRPDSDSKLKGVV 60
           M+HPI+QANENSPYG LTREEFYKKHN+THHE+FILNAQKMKIFTQSWRP SDS+LKGVV
Sbjct: 1   MAHPINQANENSPYGGLTREEFYKKHNVTHHETFILNAQKMKIFTQSWRPASDSELKGVV 60

Query: 61  AMVHGYTCDSGWIFELTGVAIAKLGFLVCSLDLQGHGRSEGAPGSIPDIELLVLDCTQFF 120
           AMVHGYT DSGW+FELT VAIAK GFLVCSLDLQGHGRS+GAPGSIPDIELLVLDCT FF
Sbjct: 61  AMVHGYTSDSGWMFELTAVAIAKSGFLVCSLDLQGHGRSDGAPGSIPDIELLVLDCTLFF 120

Query: 121 DSIREQHPNLPAFLYGESLGGAISILISLKQEGVWNGIVLNGSMCGISAKFKPIWPLEKL 180
           DS+REQ+P LPAFLYGESLGGAISILI LKQEGVW+GIVLNGSMCGISAKF+PIWPLEKL
Sbjct: 121 DSVREQNPTLPAFLYGESLGGAISILICLKQEGVWSGIVLNGSMCGISAKFRPIWPLEKL 180

Query: 181 LPIAASLAPSLRLVISKPVASKSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCEYI 240
           LPIAASLAP LRLV+SKP ASKSYKEEWKRRLVA+NPNRRFSGKPPMATALEFLRVCEYI
Sbjct: 181 LPIAASLAPWLRLVVSKPHASKSYKEEWKRRLVAKNPNRRFSGKPPMATALEFLRVCEYI 240

Query: 241 KRNCHEIRVPMLMVHGEDDVVCDSWSARYVYEAAESEDKTLKVYPGMWHQLIGETKENVE 300
           K NCHEIRVPMLMVHGEDDVVCDS SAR+V+E+A SEDKTLKVYPGMWHQLIGE  ENVE
Sbjct: 241 KMNCHEIRVPMLMVHGEDDVVCDSESARFVFESAASEDKTLKVYPGMWHQLIGEANENVE 300

Query: 301 VVYATIFNWLVDRAEKAKNNNPTKN 325
           +VYATIFNWLVDRAEKAK  N +KN
Sbjct: 301 IVYATIFNWLVDRAEKAKAKNKSKN 325

BLAST of Chy2G023530 vs. TAIR 10
Match: AT5G14980.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 345.5 bits (885), Expect = 4.7e-95
Identity = 160/325 (49.23%), Postives = 229/325 (70.46%), Query Frame = 0

Query: 3   HPISQANENSPYGELTREEFYKKHNITHHESFILNAQKMKIFTQSWRPDSDSKLKGVVAM 62
           HP+S+AN +SP+G L+  EFY +H++TH  +++ N   +K+FTQ W P +   L G++A+
Sbjct: 4   HPVSEANASSPFGSLSAAEFYSRHSVTHSSAYVTNPTGLKLFTQWWTPLNRPPL-GLIAV 63

Query: 63  VHGYTCDSGWIFELTGVAIAKLGFLVCSLDLQGHGRSEGAPGSIPDIELLVLDCTQFFDS 122
           VHG+T +S W  +LT V  AK G+L C++D QGHG S+G    IP+I L+V DC  FFD 
Sbjct: 64  VHGFTGESSWFLQLTSVLFAKSGYLTCAIDHQGHGFSDGLTAHIPNINLIVDDCISFFDD 123

Query: 123 IREQHPN--LPAFLYGESLGGAISILISLKQEGVWNGIVLNGSMCGISAKFKPIWPLEKL 182
            R++H +  LPAFLY ESLGGAI++ I+L+Q+  WNG++L+G+MC IS KFKP WPL+ L
Sbjct: 124 FRKRHASSFLPAFLYSESLGGAIALYITLRQKHQWNGLILSGAMCSISHKFKPPWPLQHL 183

Query: 183 LPIAASLAPSLRLVISK-PVASKSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCEY 242
           L +AA+L P+ R+V ++  +A  S+KE WKR+L   NPNR   GKP  ATA E +RVCE 
Sbjct: 184 LTLAATLIPTWRVVPTRGSIAGVSFKEPWKRKLAYANPNRTV-GKPRAATAYELVRVCED 243

Query: 243 IKRNCHEIRVPMLMVHGEDDVVCDSWSARYVYEAAESEDKTLKVYPGMWHQLIGETKENV 302
           ++    E+ VP+++VHG DDVVCD  S   +Y    S DKT+K+YPGMWHQLIGE++ENV
Sbjct: 244 LQNRFEEVEVPLMIVHGRDDVVCDPASVEELYRRCSSRDKTIKIYPGMWHQLIGESEENV 303

Query: 303 EVVYATIFNWLVDRAEKAKNNNPTK 325
           ++V+  + +W+  R+E +  +  TK
Sbjct: 304 DLVFGDVLDWIKTRSEISTIHGGTK 326

BLAST of Chy2G023530 vs. TAIR 10
Match: AT5G19290.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 337.0 bits (863), Expect = 1.7e-92
Identity = 157/317 (49.53%), Postives = 221/317 (69.72%), Query Frame = 0

Query: 3   HPISQANENSPYGELTREEFYKKHNITHHESFILNAQKMKIFTQSWRPDSDSKLKGVVAM 62
           HPIS ANE++P+G LT +EFY KH+++H  +FI N + +K+FTQ W P   +K  G++A+
Sbjct: 4   HPISDANEHNPFGSLTADEFYAKHSVSHSSAFITNPRGLKLFTQWWSPLPPTKPIGIIAV 63

Query: 63  VHGYTCDSGWIFELTGVAIAKLGFLVCSLDLQGHGRSEGAPGSIPDIELLVLDCTQFFDS 122
           VHG+T +S W  +LT +  AK GF+ C++D QGHG S+G    IPDI  +V DC  FFD 
Sbjct: 64  VHGFTGESSWFLQLTSILFAKSGFITCAIDHQGHGFSDGLIAHIPDINPVVDDCISFFDD 123

Query: 123 IREQH--PNLPAFLYGESLGGAISILISLKQEGVWNGIVLNGSMCGISAKFKPIWPLEKL 182
            R +    +LP FLY ESLGGAI++ ISL+Q GVW+G++LNG+MCGIS KFKP WPLE L
Sbjct: 124 FRSRQTPSDLPCFLYSESLGGAIALYISLRQRGVWDGLILNGAMCGISDKFKPPWPLEHL 183

Query: 183 LPIAASLAPSLRLVISK-PVASKSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCEY 242
           L + A+L P+ R++ ++  +   S+KE WKR+L   +P RR   KP  ATA E +RVC+ 
Sbjct: 184 LFVVANLIPTWRVIPTRGSIPDVSFKEPWKRKLAMASP-RRTVAKPRAATAYELIRVCKD 243

Query: 243 IKRNCHEIRVPMLMVHGEDDVVCDSWSARYVYEAAESEDKTLKVYPGMWHQLIGETKENV 302
           ++    E+ VP+L+VHG  DVVCD      ++  A SEDKT+K+YP +WHQ+IGE++E V
Sbjct: 244 LQGRFEEVEVPLLIVHGGGDVVCDVACVEELHRRAISEDKTIKIYPELWHQMIGESEEKV 303

Query: 303 EVVYATIFNWLVDRAEK 317
           ++VY  + +WL  RAE+
Sbjct: 304 DLVYGDMLSWLKSRAER 319

BLAST of Chy2G023530 vs. TAIR 10
Match: AT1G11090.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 242.3 bits (617), Expect = 5.6e-64
Identity = 118/303 (38.94%), Postives = 188/303 (62.05%), Query Frame = 0

Query: 14  YGELTREEFYKKHNITHHESFILNAQKMKIFTQSWRPDSDSKLKGVVAMVHGYTCDSGWI 73
           +GE + E+++    I   +SF  + + + +FT+SW P S S  +G++ MVHGY  D  W 
Sbjct: 13  WGETSEEDYFNLKGIIGSKSFFTSPRGLNLFTRSWLPSSSSPPRGLIFMVHGYGNDVSWT 72

Query: 74  FELTGVAIAKLGFLVCSLDLQGHGRSEGAPGSIPDIELLVLDCTQFFDSIRE--QHPNLP 133
           F+ T + +A++GF   +LD++GHGRS+G    +P ++L+V D   FF+SI++  +   LP
Sbjct: 73  FQSTPIFLAQMGFACFALDIEGHGRSDGVRAYVPSVDLVVDDIISFFNSIKQNPKFQGLP 132

Query: 134 AFLYGESLGGAISILISLKQEGVWNGIVLNGSMCGISAKFKPIWPLEKLLPIAASLAPSL 193
            FL+GES+GGAI +LI       ++G VL   MC IS K +P WP+++ L + +   P+ 
Sbjct: 133 RFLFGESMGGAICLLIQFADPLGFDGAVLVAPMCKISDKVRPKWPVDQFLIMISRFLPTW 192

Query: 194 RLVISKPVASKSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCEYIKRNCHEIRVPM 253
            +V ++ +  KS K E K+ +  RNP  R++ KP + T +E LRV +Y+ +   ++ +P 
Sbjct: 193 AIVPTEDLLEKSIKVEEKKPIAKRNP-MRYNEKPRLGTVMELLRVTDYLGKKLKDVSIPF 252

Query: 254 LMVHGEDDVVCDSWSARYVYEAAESEDKTLKVYPGMWH-QLIGETKENVEVVYATIFNWL 313
           ++VHG  D V D   +R +YE A+S+DKTLK+Y GM H  L GE  +N+E+V   I +WL
Sbjct: 253 IIVHGSADAVTDPEVSRELYEHAKSKDKTLKIYDGMMHSMLFGEPDDNIEIVRKDIVSWL 312

BLAST of Chy2G023530 vs. TAIR 10
Match: AT2G39420.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 207.2 bits (526), Expect = 2.0e-53
Identity = 107/287 (37.28%), Postives = 167/287 (58.19%), Query Frame = 0

Query: 27  NITHHESFILNAQKMKIFTQSWRPDSDSKLKGVVAMVHGYTCDSGWIFELTGVAIAKLGF 86
           NI + ESFI N + MK+FT  W P +  + K +V + HGY  +       T   + K GF
Sbjct: 7   NIKYEESFIKNTRGMKLFTCKWVP-AKQEPKALVFICHGYAMECSITMNSTARRLVKAGF 66

Query: 87  LVCSLDLQGHGRSEGAPGSIPDIELLVLDCTQFFDSI--REQHPNLPAFLYGESLGGAIS 146
            V  +D +GHG+S+G    +P+ + LV D +  + SI  +E++     FL GES+GGA+ 
Sbjct: 67  AVYGIDYEGHGKSDGLSAYVPNFDHLVDDVSTHYTSICEKEENKGKMRFLLGESMGGAVL 126

Query: 147 ILISLKQEGVWNGIVLNGSMCGISAKFKPIWPLEKLLPIAASLAPSLRLVISKPVASKSY 206
           +L+  K+   W+G VL   MC I+ + KP   +  +L   + + PS +++  + +   ++
Sbjct: 127 LLLHRKKPQFWDGAVLVAPMCKIAEEMKPSPLVISILAKLSGVIPSWKIIPGQDIIETAF 186

Query: 207 KEEWKRRLVARNPNRRFSGKPPMATALEFLRVCEYIKRNCHEIRVPMLMVHGEDDVVCDS 266
           K+   R+ V  NP   + G+P + TA E LRV   +++  +E+ +P +++HGEDD V D 
Sbjct: 187 KQPEIRKQVRENP-YCYKGRPRLKTAYELLRVSTDLEKRLNEVSLPFIVLHGEDDKVTDK 246

Query: 267 WSARYVYEAAESEDKTLKVYPGMWHQLI-GETKENVEVVYATIFNWL 311
             +R +YE A S DKT K+YPGMWH L+ GET EN+E V+A I  WL
Sbjct: 247 AVSRQLYEVASSSDKTFKLYPGMWHGLLYGETPENIETVFADIIGWL 291

BLAST of Chy2G023530 vs. TAIR 10
Match: AT2G47630.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 203.8 bits (517), Expect = 2.2e-52
Identity = 109/290 (37.59%), Postives = 163/290 (56.21%), Query Frame = 0

Query: 24  KKHNITHHESFILNAQKMKIFTQSWRPDSDSKLKGVVAMVHGYTCDSGWIFELTGVAIAK 83
           K     +HE ++ N++ +++F   W P S S  K +V + HGY  +     +  G+ +A 
Sbjct: 2   KSSEFQYHEEYVRNSRGVELFACRWIPSSSSSPKALVFLCHGYGMECSDSMKECGIRLAS 61

Query: 84  LGFLVCSLDLQGHGRSEGAPGSIPDIELLVLDCTQFFDSI--REQHPNLPAFLYGESLGG 143
            G+ V  +D +GHGRS G+   I     +V DC  ++ SI  +E++ +   FLYGES+GG
Sbjct: 62  AGYAVFGMDYEGHGRSMGSRCYIKKFANVVNDCYDYYTSICAQEEYMDKGRFLYGESMGG 121

Query: 144 AISILISLKQEGVWNGIVLNGSMCGISAKFKPIWPLEKLLPIAASLAPSLRLVISKPVAS 203
           A+++L+  K    WNG +L   MC IS K KP   +  LL     + P  ++V +K V  
Sbjct: 122 AVTLLLHKKDPLFWNGAILVAPMCKISEKVKPHPIVINLLTRVEEIIPKWKIVPTKDVID 181

Query: 204 KSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCEYIKRNCHEIRVPMLMVHGEDDVV 263
            ++K+  KR  V RN    +  KP + TALE LR    ++   HEI +P  ++HGE D V
Sbjct: 182 AAFKDLVKREEV-RNNKLIYQDKPRLKTALEMLRTSMNLEDTLHEITMPFFVLHGEADTV 241

Query: 264 CDSWSARYVYEAAESEDKTLKVYPGMWHQLI-GETKENVEVVYATIFNWL 311
            D   ++ +YE A + DKTLK+YPGMWH L  GE   NV++V+A I NWL
Sbjct: 242 TDPEVSKALYEKASTRDKTLKLYPGMWHALTSGEPDCNVDLVFADIINWL 290

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9C9426.7e-4632.90Caffeoylshikimate esterase OS=Arabidopsis thaliana OX=3702 GN=CSE PE=1 SV=1[more]
Q996857.7e-2629.29Monoglyceride lipase OS=Homo sapiens OX=9606 GN=MGLL PE=1 SV=2[more]
O356781.4e-2428.08Monoglyceride lipase OS=Mus musculus OX=10090 GN=Mgll PE=1 SV=1[more]
Q8R4316.1e-2327.43Monoglyceride lipase OS=Rattus norvegicus OX=10116 GN=Mgll PE=1 SV=1[more]
A0QNZ71.0e-2225.86Monoacylglycerol lipase OS=Mycolicibacterium smegmatis (strain ATCC 700084 / mc(... [more]
Match NameE-valueIdentityDescription
A0A0A0KKV99.9e-18696.65Hydrolase_4 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G525500... [more]
A0A5A7UE913.3e-18193.94Caffeoylshikimate esterase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
A0A1S3B3901.6e-18093.64caffeoylshikimate esterase-like OS=Cucumis melo OX=3656 GN=LOC103485302 PE=4 SV=... [more]
A0A6J1ECA83.8e-16988.41caffeoylshikimate esterase-like OS=Cucurbita moschata OX=3662 GN=LOC111433016 PE... [more]
A0A6J1IPF34.6e-16787.73caffeoylshikimate esterase-like OS=Cucurbita maxima OX=3661 GN=LOC111477621 PE=4... [more]
Match NameE-valueIdentityDescription
KGN49464.13.84e-23896.65hypothetical protein Csa_003642 [Cucumis sativus][more]
KAA0052486.13.65e-23293.94caffeoylshikimate esterase-like [Cucumis melo var. makuwa] >TYK13337.1 caffeoyls... [more]
XP_008441075.13.00e-23193.64PREDICTED: caffeoylshikimate esterase-like [Cucumis melo][more]
XP_038881607.17.33e-22493.13caffeoylshikimate esterase-like [Benincasa hispida][more]
XP_022925667.12.41e-21688.92caffeoylshikimate esterase-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT5G14980.14.7e-9549.23alpha/beta-Hydrolases superfamily protein [more]
AT5G19290.11.7e-9249.53alpha/beta-Hydrolases superfamily protein [more]
AT1G11090.15.6e-6438.94alpha/beta-Hydrolases superfamily protein [more]
AT2G39420.12.0e-5337.28alpha/beta-Hydrolases superfamily protein [more]
AT2G47630.12.2e-5237.59alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000073Alpha/beta hydrolase fold-1PRINTSPR00111ABHYDROLASEcoord: 251..265
score: 29.43
coord: 85..100
score: 35.56
coord: 134..147
score: 40.64
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 19..323
e-value: 1.2E-53
score: 184.2
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 40..313
IPR022742Serine aminopeptidase, S33PFAMPF12146Hydrolase_4coord: 55..295
e-value: 3.8E-55
score: 186.8
NoneNo IPR availablePANTHERPTHR11614:SF101CAFFEOYLSHIKIMATE ESTERASE-LIKEcoord: 2..319
NoneNo IPR availablePANTHERPTHR11614PHOSPHOLIPASE-RELATEDcoord: 2..319

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy2G023530.1Chy2G023530.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016020 membrane
molecular_function GO:0016298 lipase activity