Chy2G023300 (gene) Cucumber (hystrix) v1

Overview
NameChy2G023300
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
Descriptionalpha N-terminal protein methyltransferase 1
LocationchrH02: 469873 .. 474955 (+)
RNA-Seq ExpressionChy2G023300
SyntenyChy2G023300
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAGGGAGCGGTGCTGATACAGACGGCCATGAATTCAAGAATGCGGAGGAGATGTGGAGGGAACATGTTGGAAACCCCACTAAGCGCACAGAATGGTATCGTGAAGGTGTAGGCTATTGGCAAGGTGTTGAGGCTTCTGTTGATGGGGTGTTGGGCGGCTATGGCCATGTTAACGACGCCGATATATTGGGTAGTGAAGTTTTTCTCAAATCCATTTTGGTCGAGCGTTTCTCTTTTGCTGGAAAAGACCGCCCCCTTGTTGCTCTTGGTATCTCTCTTTTTACCCATTTCTTACTTTTCTCATTCCATCACTTTTGCTTATAACTGCTCTGGATGAGTTGTACTTTTCAGTTAATGCTACTAATGCTTGAGCTCCTATTCCAGATTGTGGTTCTGGCATTGGGAGAGTCACCAAGAATCTTCTTATTAAATATTTTAACGAGGTAACGTAAGATAATACAAATTGCCTTTTTGTTCCTGCCATGTTACTTTCTTACTTTCTATCTGCCTGTCTTGGAATCCGTGTGTAACTTTTACGAAAGCGCGTTTACTTTGGTTTTTAGCTTTTGGTTTTTCTTCGTCTTCGTGCAATCTGCTTGGCAGCTTGAAAGGTGGACATGATCATATGGATAGATGTTCAGTTTTCACAAGAGTGGCTAGAAGAACTCTTATTGAGCTGTCGGGGATTTAATGCTCGTTTTTTTTTCTTTTCTTTTTAAAAAAATGTGTATTGAAGAACCAAACGACTGATGAATATGGTGGAATAGCTGTTAAATTAATTGAATTGCAGCGCTTCCATATATTTTTTAATATAAAAGGAAGTAAGATCACAAACGTTTAGTGGTAAAGTAACCGTGTGGAACTTAGATGGAATTTTGATGCTTTTGCGCCAATAATGCAGTGAACGTAAAATTGGCGGTCGGATGAACTGTAGGGCATCCTGTCTTCTATCCACATGAAACACTGGAACGTTGTTGGATATTGTCATATATAAGATAAGAACAATGAGATCAATAACCAACTCACTTTATAATAAATTTAGTTTCCCTGTGCATACCCCTGTCAGAATAACATTATTGAGGTTGGGCCATTTCTGTATGACCATTTCCTTGACTTCAAAAAATGGTTTTCCGGTTATTGACATGCTCTGATGTAGACTGTCATGATTCTGGAATCAATGGTTTGTTGTGACTCTAGATCCAATTTTCCCAAGCGTTTAATTCCACATTCTACTTTATGTGAATTGCAGCAAGAGATCGCTACTAGCTTCTTTTGTTTTTTGATGTGAAAACTTTACTCCTACAGATAATTTGTTTATTTACCTCTTTTTGAGTCTTAGGTTGATCTTCTTGAACCTGTATCACATTTCCTAGAGGCTGCTCGTGGAAACTTGGCTCCAGAAAATAATGGGCCCTCTGATCTGCACAAAGCCACTAACTTCTTTTGCATGCCTCTACAGGTATATGTAATCAATTGATATTCAATCAGGTAAAATAAGAGAATTTGTTATTTTTTGTGGTTGTGTTTGCTCAAGACCTTCTCTTTCGGTGTTACTTGGTAGCTTGGTAGCTTATGTACCCTCTAGCTTGGTAGCTTCTCTTTCGGTGTTAATTTAGGTGTTACTTGGTAGCTTATGTACCCTTTTTTAATATGAAAGGAATTTTGGTTTTTGTGTAAAGAGCACGTCCTCCCCCACTATGAAACTGAAAAGAGAAAAGTTGTAAGAAACTGGAGAACAATTGCCAATATGAGCATAGGTCCATGGTTAGGACAATATTGTCGAACTCAAGTAAAAAGAAACGGGGAATCAATTTTTAACATTGCTAGATGCCACCGGATAGAGGTTCAACATCCAGTAGGTGAGACATTGTATTGAGAAGTTTTATGTGTAAGTTGCTTTGGTATCTTAGTTGATGCTACGGTGCCTTTGGATCTCCTCCATCTTCTGCCTGTGCATATCTGATTTACAGTAGTAAATTATGAATACCAAATGTCTATCTGCCTGAATTTTCTTTGACATCCATGAATTCGGCTGTATATTTCTTGATTTAGGGTGCCAGTCGTTTTCTCTTTTCTAAAACATTTAGATTTACACTACTGCTAGGAATTCACGCCAGATGCAGGAAGATATGATGTTATTTGGGTTCAGTGGTGTATTGGACATCTCACTGATGAGGACTTTATCTCGTTCTTTAAAAGAGCAAAGGTAAGTTGTGTATATATATTTTTTTCTTGAATCAAGCTCTGAAGAATTTTGTTACTTGAGTAATTTATCATTTCTCGGGTCCCTTTGGCTGTAGTAATGTGATTTAGCTGTCAGTGTCAGTTAAACCCTTCAAATGGTTTAGATCTTCTCATTTTTCTCTTACCTAATCTTTAAATTTCTTGTTTGCCACCTTTGTTGTTGTGGAGTTCTTCTATTGTGGGTGATATATTTTGAATTAGCTACTAAAACAGTCTTGAATATGGAAATGATAATGATGAAGGCCAATATGATGTTCACATCAGAGACTAAGATGTCGTGAAAATGTCAACAGAAATACTGGCAACAAAGTTTACTTCATGGAAGCTTGAAACCTTTAGATTCAAACCTAAAATTTCAATTTATATATAACTTTAGAAAGATATTAAAGAAACTTTATGTATAATGAAATTATGAAACTTTATGTCTAAAAATTTCAATATTTCAAATAGTATCTCAGGAAAATATCTTTTTGATAATATTTATATTGAAAATAGAGATCTTAATATTATATCTATTAGAAACTAGAAGCTCATTTCTCAGAATAAAATAGAAATACGAGGGTAGATAAGATATGTTCACCTAGTTAAGGTTTGTATAAAGGACTTTGAATCGGCATTGATGTGAGTGCAAACGGAAAAAAAAGGTGCATACTGGAATTTTCAGGTCGAAAGCCTAGCAAACATCTCAATCACCTCTGGATCTCTTGCCTTTTGTTTGTGACTTTTTCTCTGTCCAAACTCCCCAGAATAGCTGTGAATTGATTTCTCAAAAAACGTAGGCACCTCTTTTGAACACTGAAAGATGAAACGATCATTCAATTGTGGGTATTGACTTGAAAATCATTCTCAGTGAGTCTTATTATAGTTCTTTTTTTTCACGGTTTTAAGTTTTCCTTTCACATTGGTGTTTTTTTTTTTTGGGAAGAACACGTTGATAGTTGTTCATGTTTGAATATTTAAGAATTAAGTATGCCATGACTGCATCAGGTAGACTATAACTTATTATGGCTCTAATCATTCATTACTTTTCAGTTGGGCCTAAAAGCTGGCGGAATTTTCATCCTGAAGGAAAATATTGCAAGATCTGGTACTTGCCATTTTCAGTTTTTTTCTTTATGAATCCCATACTATCTAGTAACAAGAATATATTAGTTCCTAAAAGAAAAAAGTAACAAGAATATATTATTTCAATTTCAATGTCTGGTACCTGCATTCAATGAAAATGATTTTCATTGTTTCACCTCCAACAAAGTTTTTGGTTTTGAGAAAGACCTTTTTATTTAGAGCATGTCTGCCTTATTAACCTTTCGAGTGGGACATATCAGAACTCAAGTGTTGTATCTTGTTGAAGGGTTTGTATTAGATAAAGAAGATCGAAGCATCACAAGATCTGACTCATACTATAAAGATCTATTCAACCAGTGCGGACTCTACATATTTAAATCAAGGGTAAAGTCACTATCCATTTGGTTCAGTCTAATCTATGCTACTTTTGTTTATTTTCGTCTCTTCAACCATCAAACAGAATTGCAAATGATTTTCTCGGTTATTTTATTTTAAGTCCATTATTTATTTCTCAAATTCATCTTTGCAAATCACTTCTAATAACAAGATATTTAAGATAGATGTAATTTCATCTGATTATGTTCATAAAAGGCTCTTAAATTTCTACACTTCCAGTAGGATGCCTTCTGATCTCAAAATATTGAAATTAGTCCTGAGAAGTTGTAAACTAGCTCTATCAGCATTTCTGCTTTACCTCACTGTACTTGGTTTATTCAAATTCCCTTTGGTCCTATGGAAAACAATGGATTTAACAGAAAATAGAGTTATATTTCATGTTTGAAATGTAAACTAGTTAGAGAGTTCGATAGCAACTTGAACCATAAGGTGAGCTAAGACCCATCATTTTAAAGAAAAGCATACATTACCCATAAAAGGATTGGAATACTTAGATTGAATATGAATAGATTCGTAACCAGGACCTCCCAACTCAATCCATGAACATGCTTTTACCTAGGTTTACCAACTCTGAAATTCCTCCTATCTCGGTGGATTGATTGTTTTCTATTTTGTGGTTAATGTTTTTAGGACCAAAAGGGATTTCCGCAGGAATTATTTCCTGTGAAGATGTACGCATTAACTACAGAGGCCCCCAAGAGGAGTTCTCGCATCAAAAGGGAACAAAGCAATAGACCTGGCGTTATCAAGTGAGGAGCTTCGCCTCATTTCTCTCCTTTCTGAATGAGCCAGTAGCAATCAATATATACATTTGTAGACGGGTACTTTTTAACCTTTTATGTAGATTGCATGTCTGTGTAAGTACATATTATCCCAAAATTCTCGTAGAATGTCATCTTGTCTTCAAGTGATAAGTATGACCTACCTCCCTTAGTCTTTGTTAACTCTCTGGCTTTGATTGTTGAATACTCCCGTTTTAAAGATCTGAACAAAAGCAAATTACAAAACCCAAGTTTGTTTTAGAGAATGATTTCTTGGTCCAACTATTTTAACCTATTTCTACACGTCCTGGACTGTTTGGAAAGGTATCTTCATTTGTAGTTGAAGATAAACTGCTTGTCTTGCAAACAAATACTTCTTGAAACAGAAACAAACGCTGAAAGACAGTAAAATTACACTTATATTAAAAGATCTGTATATATTTGGTATACATCGATATTTTAATTTCTCAGTTGTATTTATTTTGTGTGGTAGGCCAGTTGACATACAAATCCATAGTAGATGGAACTGGAAACACGAAGCTTCTCGATTTTCATACTTGCAAGGAGACGCCATTGCCATCCGCCCCAAAGCCCTTTTCCCTTTGTTATGTTAA

mRNA sequence

ATGGAAGGGAGCGGTGCTGATACAGACGGCCATGAATTCAAGAATGCGGAGGAGATGTGGAGGGAACATGTTGGAAACCCCACTAAGCGCACAGAATGGTATCGTGAAGGTGTAGGCTATTGGCAAGGTGTTGAGGCTTCTGTTGATGGGGTGTTGGGCGGCTATGGCCATGTTAACGACGCCGATATATTGGGTAGTGAAGTTTTTCTCAAATCCATTTTGGTCGAGCGTTTCTCTTTTGCTGGAAAAGACCGCCCCCTTGTTGCTCTTGATTGTGGTTCTGGCATTGGGAGAGTCACCAAGAATCTTCTTATTAAATATTTTAACGAGGTTGATCTTCTTGAACCTGTATCACATTTCCTAGAGGCTGCTCGTGGAAACTTGGCTCCAGAAAATAATGGGCCCTCTGATCTGCACAAAGCCACTAACTTCTTTTGCATGCCTCTACAGGAATTCACGCCAGATGCAGGAAGATATGATGTTATTTGGGTTCAGTGGTGTATTGGACATCTCACTGATGAGGACTTTATCTCGTTCTTTAAAAGAGCAAAGTTGGGCCTAAAAGCTGGCGGAATTTTCATCCTGAAGGAAAATATTGCAAGATCTGGGTTTGTATTAGATAAAGAAGATCGAAGCATCACAAGATCTGACTCATACTATAAAGATCTATTCAACCAGTGCGGACTCTACATATTTAAATCAAGGGACCAAAAGGGATTTCCGCAGGAATTATTTCCTGTGAAGATGTACGCATTAACTACAGAGGCCCCCAAGAGGAGTTCTCGCATCAAAAGGGAACAAAGCAATAGACCTGGCGTTATCAAGCCAGTTGACATACAAATCCATAGTAGATGGAACTGGAAACACGAAGCTTCTCGATTTTCATACTTGCAAGGAGACGCCATTGCCATCCGCCCCAAAGCCCTTTTCCCTTTGTTATGTTAA

Coding sequence (CDS)

ATGGAAGGGAGCGGTGCTGATACAGACGGCCATGAATTCAAGAATGCGGAGGAGATGTGGAGGGAACATGTTGGAAACCCCACTAAGCGCACAGAATGGTATCGTGAAGGTGTAGGCTATTGGCAAGGTGTTGAGGCTTCTGTTGATGGGGTGTTGGGCGGCTATGGCCATGTTAACGACGCCGATATATTGGGTAGTGAAGTTTTTCTCAAATCCATTTTGGTCGAGCGTTTCTCTTTTGCTGGAAAAGACCGCCCCCTTGTTGCTCTTGATTGTGGTTCTGGCATTGGGAGAGTCACCAAGAATCTTCTTATTAAATATTTTAACGAGGTTGATCTTCTTGAACCTGTATCACATTTCCTAGAGGCTGCTCGTGGAAACTTGGCTCCAGAAAATAATGGGCCCTCTGATCTGCACAAAGCCACTAACTTCTTTTGCATGCCTCTACAGGAATTCACGCCAGATGCAGGAAGATATGATGTTATTTGGGTTCAGTGGTGTATTGGACATCTCACTGATGAGGACTTTATCTCGTTCTTTAAAAGAGCAAAGTTGGGCCTAAAAGCTGGCGGAATTTTCATCCTGAAGGAAAATATTGCAAGATCTGGGTTTGTATTAGATAAAGAAGATCGAAGCATCACAAGATCTGACTCATACTATAAAGATCTATTCAACCAGTGCGGACTCTACATATTTAAATCAAGGGACCAAAAGGGATTTCCGCAGGAATTATTTCCTGTGAAGATGTACGCATTAACTACAGAGGCCCCCAAGAGGAGTTCTCGCATCAAAAGGGAACAAAGCAATAGACCTGGCGTTATCAAGCCAGTTGACATACAAATCCATAGTAGATGGAACTGGAAACACGAAGCTTCTCGATTTTCATACTTGCAAGGAGACGCCATTGCCATCCGCCCCAAAGCCCTTTTCCCTTTGTTATGTTAA

Protein sequence

MEGSGADTDGHEFKNAEEMWREHVGNPTKRTEWYREGVGYWQGVEASVDGVLGGYGHVNDADILGSEVFLKSILVERFSFAGKDRPLVALDCGSGIGRVTKNLLIKYFNEVDLLEPVSHFLEAARGNLAPENNGPSDLHKATNFFCMPLQEFTPDAGRYDVIWVQWCIGHLTDEDFISFFKRAKLGLKAGGIFILKENIARSGFVLDKEDRSITRSDSYYKDLFNQCGLYIFKSRDQKGFPQELFPVKMYALTTEAPKRSSRIKREQSNRPGVIKPVDIQIHSRWNWKHEASRFSYLQGDAIAIRPKALFPLLC*
Homology
BLAST of Chy2G023300 vs. ExPASy Swiss-Prot
Match: Q5PP70 (Alpha N-terminal protein methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=At5g44450 PE=2 SV=1)

HSP 1 Score: 397.1 bits (1019), Expect = 1.8e-109
Identity = 188/279 (67.38%), Postives = 231/279 (82.80%), Query Frame = 0

Query: 1   MEGSGADTDGHEFKNAEEMWREHV--GNPTKRTEWYREGVGYWQGVEASVDGVLGGYGHV 60
           M+  G D++G EF + +EMWRE +  G+ TK+T+WYR+GV YW+GVEASVDGVLGGYGHV
Sbjct: 1   MDICGVDSEGKEFNSVQEMWREEIGEGDETKKTQWYRDGVSYWEGVEASVDGVLGGYGHV 60

Query: 61  NDADILGSEVFLKSILVERF-SFAGKDRPLVALDCGSGIGRVTKNLLIKYFNEVDLLEPV 120
           NDADI+GSEVFLK++L ER  +  G ++ LVALDCGSGIGR+TKNLLI+YFNEVDLLEPV
Sbjct: 61  NDADIIGSEVFLKTLLQERLVNNVGANQHLVALDCGSGIGRITKNLLIRYFNEVDLLEPV 120

Query: 121 SHFLEAARGNLAPENNGPSDLHKATNFFCMPLQEFTPDAGRYDVIWVQWCIGHLTDEDFI 180
           + FL+AAR NLA   +  S+ HKATNFFC+PLQEFTP AGRYDVIWVQWCIGHLTD DF+
Sbjct: 121 AQFLDAARENLA---SAGSETHKATNFFCVPLQEFTPAAGRYDVIWVQWCIGHLTDNDFV 180

Query: 181 SFFKRAKLGLKAGGIFILKENIARSGFVLDKEDRSITRSDSYYKDLFNQCGLYIFKSRDQ 240
           SFF RAK  LK GG F++KEN+A++GFVLDKED SITRSD Y+K LF QCGL++++++DQ
Sbjct: 181 SFFNRAKGYLKPGGFFVVKENLAKNGFVLDKEDHSITRSDPYFKQLFRQCGLHLYRTKDQ 240

Query: 241 KGFPQELFPVKMYALTTEAPKRSSRIK-REQSNRPGVIK 276
           KG PQELF VKMYALT + P +  R + + +SNRP +IK
Sbjct: 241 KGLPQELFAVKMYALTVDTPPKIHRTRSKTRSNRPQIIK 276

BLAST of Chy2G023300 vs. ExPASy Swiss-Prot
Match: Q10CT5 (Alpha N-terminal protein methyltransferase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0780900 PE=2 SV=2)

HSP 1 Score: 349.7 bits (896), Expect = 3.3e-95
Identity = 174/308 (56.49%), Postives = 207/308 (67.21%), Query Frame = 0

Query: 1   MEGSGADTDGHEFKNAEEMW--------------------------------REHVGNPT 60
           M+  G D++G EF +A EMW                                 E  G   
Sbjct: 1   MDSRGFDSEGREFSSATEMWAHEIGAAADAPVSAAVAEPAPAPAAGSNGVAGEEEAGGGG 60

Query: 61  KRTEWYREGVGYWQGVEASVDGVLGGYGHVNDADILGSEVFLKSILVERFSFAGKDRPLV 120
           KR EWY + + YWQGVEAS +GVLGGYG VND D+ GS+ FL+ +L ERF  A   R LV
Sbjct: 61  KREEWYSKAIAYWQGVEASTEGVLGGYGCVNDVDVKGSDAFLRPLLAERFGAA--RRHLV 120

Query: 121 ALDCGSGIGRVTKNLLIKYFNEVDLLEPVSHFLEAARGNLAPENNGPSDLHKATNFFCMP 180
           ALDCGSGIGRVTKN L+++FNEVDL+EPVSHFLEAA+ NL        D HKA NF+C+P
Sbjct: 121 ALDCGSGIGRVTKNFLLRHFNEVDLVEPVSHFLEAAQENLTECMEVGEDTHKAANFYCVP 180

Query: 181 LQEFTPDAGRYDVIWVQWCIGHLTDEDFISFFKRAKLGLKAGGIFILKENIARSGFVLDK 240
           LQ+FTPD GRYDVIW+QWCIG L D+DFISFF RAK+GLK  G F+LKENIAR+GFVLDK
Sbjct: 181 LQDFTPDEGRYDVIWIQWCIGQLPDDDFISFFNRAKIGLKPNGFFVLKENIARNGFVLDK 240

Query: 241 EDRSITRSDSYYKDLFNQCGLYIFKSRDQKGFPQELFPVKMYALTTEAPKRSSRIKREQ- 276
           ED SITRSD+Y+K+LF +CGLYI   +DQ   P+ELF VKMYAL TE PK     KR + 
Sbjct: 241 EDNSITRSDAYFKELFKKCGLYIHSIKDQSDLPKELFAVKMYALVTEKPKIQKNGKRRRP 300

BLAST of Chy2G023300 vs. ExPASy Swiss-Prot
Match: A2XMJ1 (Alpha N-terminal protein methyltransferase 1 OS=Oryza sativa subsp. indica OX=39946 GN=OsI_13745 PE=3 SV=1)

HSP 1 Score: 349.0 bits (894), Expect = 5.7e-95
Identity = 173/308 (56.17%), Postives = 207/308 (67.21%), Query Frame = 0

Query: 1   MEGSGADTDGHEFKNAEEMWREHVGNPT-------------------------------- 60
           M+  G D++G EF +A EMW   +G                                   
Sbjct: 1   MDSRGFDSEGREFSSATEMWAHEIGAAADAPVSAAVAEPAPAPAAGSNGVAGEQEAGGGG 60

Query: 61  KRTEWYREGVGYWQGVEASVDGVLGGYGHVNDADILGSEVFLKSILVERFSFAGKDRPLV 120
           KR EWY + + YWQGVEAS +GVLGGYG VND D+ GS+ FL+ +L ERF  A   R LV
Sbjct: 61  KREEWYSKAIAYWQGVEASTEGVLGGYGCVNDVDVKGSDAFLRPLLAERFGAA--RRHLV 120

Query: 121 ALDCGSGIGRVTKNLLIKYFNEVDLLEPVSHFLEAARGNLAPENNGPSDLHKATNFFCMP 180
           ALDCGSGIGRVTKN L+++FNEVDL+EPVSHFLEAA+ NL        D HKA NF+C+P
Sbjct: 121 ALDCGSGIGRVTKNFLLRHFNEVDLVEPVSHFLEAAQENLTECMEVGEDTHKAANFYCVP 180

Query: 181 LQEFTPDAGRYDVIWVQWCIGHLTDEDFISFFKRAKLGLKAGGIFILKENIARSGFVLDK 240
           LQ+FTPD GRYDVIW+QWCIG L D+DFISFF RAK+GLK  G F+LKENIAR+GFVLDK
Sbjct: 181 LQDFTPDEGRYDVIWIQWCIGQLPDDDFISFFNRAKIGLKPNGFFVLKENIARNGFVLDK 240

Query: 241 EDRSITRSDSYYKDLFNQCGLYIFKSRDQKGFPQELFPVKMYALTTEAPKRSSRIKREQ- 276
           ED SITRSD+Y+K+LF +CGLYI   +DQ   P+ELF VKMYAL TE PK     KR + 
Sbjct: 241 EDNSITRSDAYFKELFKKCGLYIHSIKDQSDLPKELFAVKMYALVTEKPKIQKNGKRRRP 300

BLAST of Chy2G023300 vs. ExPASy Swiss-Prot
Match: Q55DH6 (Alpha N-terminal protein methyltransferase 1 OS=Dictyostelium discoideum OX=44689 GN=DDB_G0269658 PE=3 SV=1)

HSP 1 Score: 198.0 bits (502), Expect = 1.6e-49
Identity = 104/257 (40.47%), Postives = 156/257 (60.70%), Query Frame = 0

Query: 4   SGADTDGHEFKNAEEMWREHVGNPTKRTE--WYREGVGYWQGVEASVDGVLGGYGHVNDA 63
           SG D +G+ + N E++W++ +     + E  WY+    YW+GVEA+VDG+LGG   V+  
Sbjct: 24  SGLDGEGNTYINIEDLWKKELEGKDNKMEDKWYKSADEYWKGVEATVDGMLGGLAQVSPI 83

Query: 64  DILGSEVFLKSILVERFSFAGKD-RPLV----ALDCGSGIGRVTKNLLIKY-FNEVDLLE 123
           D++ S+VF++  +       G D RP +    ALDCG+GIGRV K  L+   F  VDL+E
Sbjct: 84  DVVASKVFIQDFI------KGTDSRPPINLNLALDCGAGIGRVAKEFLLPIGFKNVDLVE 143

Query: 124 PVSHFLEAARGNLAPENNGPSDLHKATNFFCMPLQEFTPDAGRYDVIWVQWCIGHLTDED 183
               FL+ A+ +   ++N      +  N++ + LQ+FT +  +YD IW+QW IGHL D D
Sbjct: 144 QNKLFLDKAKSDNFKDDN------RVENYYAVGLQDFTFEK-KYDCIWIQWVIGHLHDLD 203

Query: 184 FISFFKRAKLGLKAGGIFILKENIARSGFVLDKEDRSITRSDSYYKDLFNQCGLYIFKSR 243
           FI F K+    L   GI  +K+N A+  F++DKED S++R++ + K LF+Q G  + KS 
Sbjct: 204 FIEFLKKCMDSLTPNGIICIKDNCAKKRFIMDKEDNSVSRTEDHLKYLFDQAGCKLLKSM 263

Query: 244 DQKGFPQELFPVKMYAL 253
            Q  FP+ELFPV M+AL
Sbjct: 264 VQPNFPKELFPVLMFAL 267

BLAST of Chy2G023300 vs. ExPASy Swiss-Prot
Match: Q4KLE6 (N-terminal Xaa-Pro-Lys N-methyltransferase 1-B OS=Xenopus laevis OX=8355 GN=ntmt1-b PE=2 SV=1)

HSP 1 Score: 197.2 bits (500), Expect = 2.8e-49
Identity = 92/222 (41.44%), Postives = 133/222 (59.91%), Query Frame = 0

Query: 31  TEWYREGVGYWQGVEASVDGVLGGYGHVNDADILGSEVFLKSILVERFSFAGKDRPLVAL 90
           T++Y +   YW+ V A+VDG+LGGYGH+++ D+  S+ FL+  L E     G      AL
Sbjct: 10  TQFYGKAQNYWKNVPATVDGMLGGYGHISNTDLNSSKKFLQRFLREGSQKTGN---TCAL 69

Query: 91  DCGSGIGRVTKNLLIKYFNEVDLLEPVSHFLEAARGNLAPENNGPSDLHKATNFFCMPLQ 150
           DCG+GIGR+TK LL+  F  VD+++    FL  A+  L  E        +   +FC  LQ
Sbjct: 70  DCGAGIGRITKRLLLPLFKTVDMVDVTDEFLNKAKSYLGEEGK------RVGKYFCCGLQ 129

Query: 151 EFTPDAGRYDVIWVQWCIGHLTDEDFISFFKRAKLGLKAGGIFILKENIARSGFVLDKED 210
           EF+P+  RYDVIW+QW IGHLTDE  +SF +R KLGL+  GI ++K+N+ + G ++D  D
Sbjct: 130 EFSPEPSRYDVIWIQWVIGHLTDEHLVSFLQRCKLGLRPNGIILIKDNVTQDGSIMDDAD 189

Query: 211 RSITRSDSYYKDLFNQCGLYIFKSRDQKGFPQELFPVKMYAL 253
            SI R     + L  Q GL +     Q+ FP E++ V  +A+
Sbjct: 190 SSICRDIDLVRKLIKQAGLSVLAIERQENFPDEIYQVFSFAM 222

BLAST of Chy2G023300 vs. ExPASy TrEMBL
Match: A0A0A0KIY9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G525730 PE=3 SV=1)

HSP 1 Score: 657.9 bits (1696), Expect = 2.1e-185
Identity = 312/314 (99.36%), Postives = 314/314 (100.00%), Query Frame = 0

Query: 1   MEGSGADTDGHEFKNAEEMWREHVGNPTKRTEWYREGVGYWQGVEASVDGVLGGYGHVND 60
           MEGSGADTDGHEFKNAEEMWREHVGNPTKRTEWYREGVGYWQGVEASVDGVLGGYGHVND
Sbjct: 1   MEGSGADTDGHEFKNAEEMWREHVGNPTKRTEWYREGVGYWQGVEASVDGVLGGYGHVND 60

Query: 61  ADILGSEVFLKSILVERFSFAGKDRPLVALDCGSGIGRVTKNLLIKYFNEVDLLEPVSHF 120
           ADILGSEVFLKSILVERFSFAGKDRPLVALDCGSGIGRVTKNLLIKYFNEVDLLEPVSHF
Sbjct: 61  ADILGSEVFLKSILVERFSFAGKDRPLVALDCGSGIGRVTKNLLIKYFNEVDLLEPVSHF 120

Query: 121 LEAARGNLAPENNGPSDLHKATNFFCMPLQEFTPDAGRYDVIWVQWCIGHLTDEDFISFF 180
           LEAARGNLAPENNGPSDLHKATNFFCMPLQEFTPDAGRYDVIWVQWCIGHLTDEDFISFF
Sbjct: 121 LEAARGNLAPENNGPSDLHKATNFFCMPLQEFTPDAGRYDVIWVQWCIGHLTDEDFISFF 180

Query: 181 KRAKLGLKAGGIFILKENIARSGFVLDKEDRSITRSDSYYKDLFNQCGLYIFKSRDQKGF 240
           KRAKLGLKAGGIFILKENIARSGFVLDKEDRSITRSDSYYKDLFNQCGLYIFKSRDQKGF
Sbjct: 181 KRAKLGLKAGGIFILKENIARSGFVLDKEDRSITRSDSYYKDLFNQCGLYIFKSRDQKGF 240

Query: 241 PQELFPVKMYALTTEAPKRSSRIKREQSNRPGVIKPVDIQIHSRWNWKHEASRFSYLQGD 300
           PQELFPVKMYALTTEAPKRSSRIKREQSNRPGVIKPVDIQIHSRWNWKH+ASRFSYLQGD
Sbjct: 241 PQELFPVKMYALTTEAPKRSSRIKREQSNRPGVIKPVDIQIHSRWNWKHQASRFSYLQGD 300

Query: 301 AIAIRPKALFPLLC 315
           AIAIRPKAL+PLLC
Sbjct: 301 AIAIRPKALYPLLC 314

BLAST of Chy2G023300 vs. ExPASy TrEMBL
Match: A0A1S3AZL9 (alpha N-terminal protein methyltransferase 1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103484292 PE=3 SV=1)

HSP 1 Score: 647.1 bits (1668), Expect = 3.7e-182
Identity = 305/314 (97.13%), Postives = 310/314 (98.73%), Query Frame = 0

Query: 1   MEGSGADTDGHEFKNAEEMWREHVGNPTKRTEWYREGVGYWQGVEASVDGVLGGYGHVND 60
           MEGSGADTDGHEFKNAEEMWREHVGNPTKRTEWYREGVGYWQGVEASVDGVLGGYGHVND
Sbjct: 1   MEGSGADTDGHEFKNAEEMWREHVGNPTKRTEWYREGVGYWQGVEASVDGVLGGYGHVND 60

Query: 61  ADILGSEVFLKSILVERFSFAGKDRPLVALDCGSGIGRVTKNLLIKYFNEVDLLEPVSHF 120
           ADILGSEVFLKSIL ER SFAGKDRPLVALDCGSGIGR+TKNLLI+YFNEVDLLEPVSHF
Sbjct: 61  ADILGSEVFLKSILGERLSFAGKDRPLVALDCGSGIGRITKNLLIRYFNEVDLLEPVSHF 120

Query: 121 LEAARGNLAPENNGPSDLHKATNFFCMPLQEFTPDAGRYDVIWVQWCIGHLTDEDFISFF 180
           LEAARG+LAPENNGPSDLHKATNFFCMPLQEFTPDAGRYDVIWVQWCIGHLTDEDFISFF
Sbjct: 121 LEAARGSLAPENNGPSDLHKATNFFCMPLQEFTPDAGRYDVIWVQWCIGHLTDEDFISFF 180

Query: 181 KRAKLGLKAGGIFILKENIARSGFVLDKEDRSITRSDSYYKDLFNQCGLYIFKSRDQKGF 240
           KRAKLGLKAGGIFILKENIARSGF+LDKEDRSITRSDSYYKDLFNQCGLYIFKSRDQKGF
Sbjct: 181 KRAKLGLKAGGIFILKENIARSGFILDKEDRSITRSDSYYKDLFNQCGLYIFKSRDQKGF 240

Query: 241 PQELFPVKMYALTTEAPKRSSRIKREQSNRPGVIKPVDIQIHSRWNWKHEASRFSYLQGD 300
           PQELFPVKMYALTTE PKRSSRIKREQSNRPGVIKPVDIQIHSRWNWKH+ASRFSYLQGD
Sbjct: 241 PQELFPVKMYALTTEVPKRSSRIKREQSNRPGVIKPVDIQIHSRWNWKHQASRFSYLQGD 300

Query: 301 AIAIRPKALFPLLC 315
            IAIRPKALFPLLC
Sbjct: 301 VIAIRPKALFPLLC 314

BLAST of Chy2G023300 vs. ExPASy TrEMBL
Match: A0A6J1CY14 (alpha N-terminal protein methyltransferase 1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111015851 PE=3 SV=1)

HSP 1 Score: 565.5 bits (1456), Expect = 1.4e-157
Identity = 270/315 (85.71%), Postives = 290/315 (92.06%), Query Frame = 0

Query: 1   MEGSGADTDGHEFKNAEEMWREHVGNPTKRTEWYREGVGYWQGVEASVDGVLGGYGHVND 60
           ME SGADTDGHEFKNAEEMW E VGNPTKRT+WYREGVGYWQGVEASVDGVLGGYGHVND
Sbjct: 1   MEVSGADTDGHEFKNAEEMWTEQVGNPTKRTQWYREGVGYWQGVEASVDGVLGGYGHVND 60

Query: 61  ADILGSEVFLKSILVERFSFAGKDRPLVALDCGSGIGRVTKNLLIKYFNEVDLLEPVSHF 120
           ADILGSEVFLKSIL ERF FAGKDRPLVALDCGSGIGRVTKNLLI+YFNEVDLLEPVSHF
Sbjct: 61  ADILGSEVFLKSILSERFHFAGKDRPLVALDCGSGIGRVTKNLLIRYFNEVDLLEPVSHF 120

Query: 121 LEAARGNLAPENNG-PSDLHKATNFFCMPLQEFTPDAGRYDVIWVQWCIGHLTDEDFISF 180
           LEAAR +LAPEN+G  SD+HKATNFFC+PLQEFTP AGRYD+IWVQWCIGHLTDEDFISF
Sbjct: 121 LEAARESLAPENSGASSDMHKATNFFCVPLQEFTPAAGRYDIIWVQWCIGHLTDEDFISF 180

Query: 181 FKRAKLGLKAGGIFILKENIARSGFVLDKEDRSITRSDSYYKDLFNQCGLYIFKSRDQKG 240
           FKRAK GLK GGIFILKENIARSGF+LDKEDRSITRSD YYK+LFNQCGLY FKS+DQKG
Sbjct: 181 FKRAKAGLKGGGIFILKENIARSGFILDKEDRSITRSDIYYKNLFNQCGLYPFKSKDQKG 240

Query: 241 FPQELFPVKMYALTTEAPKRSSRIKREQSNRPGVIKPVDIQIHSRWNWKHEASRFSYLQG 300
           FP+ELFPVKMYALTTE PKR+SR KREQSNRPG+IKP++IQI +R NWK +AS FS ++G
Sbjct: 241 FPEELFPVKMYALTTEGPKRNSRTKREQSNRPGIIKPLNIQIRTRLNWKPQASGFSCIRG 300

Query: 301 DAIAIRPKALFPLLC 315
           DAIAIRP +    LC
Sbjct: 301 DAIAIRPNSKPLTLC 315

BLAST of Chy2G023300 vs. ExPASy TrEMBL
Match: A0A1S3AYJ5 (alpha N-terminal protein methyltransferase 1 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103484292 PE=3 SV=1)

HSP 1 Score: 564.3 bits (1453), Expect = 3.2e-157
Identity = 268/275 (97.45%), Postives = 272/275 (98.91%), Query Frame = 0

Query: 1   MEGSGADTDGHEFKNAEEMWREHVGNPTKRTEWYREGVGYWQGVEASVDGVLGGYGHVND 60
           MEGSGADTDGHEFKNAEEMWREHVGNPTKRTEWYREGVGYWQGVEASVDGVLGGYGHVND
Sbjct: 1   MEGSGADTDGHEFKNAEEMWREHVGNPTKRTEWYREGVGYWQGVEASVDGVLGGYGHVND 60

Query: 61  ADILGSEVFLKSILVERFSFAGKDRPLVALDCGSGIGRVTKNLLIKYFNEVDLLEPVSHF 120
           ADILGSEVFLKSIL ER SFAGKDRPLVALDCGSGIGR+TKNLLI+YFNEVDLLEPVSHF
Sbjct: 61  ADILGSEVFLKSILGERLSFAGKDRPLVALDCGSGIGRITKNLLIRYFNEVDLLEPVSHF 120

Query: 121 LEAARGNLAPENNGPSDLHKATNFFCMPLQEFTPDAGRYDVIWVQWCIGHLTDEDFISFF 180
           LEAARG+LAPENNGPSDLHKATNFFCMPLQEFTPDAGRYDVIWVQWCIGHLTDEDFISFF
Sbjct: 121 LEAARGSLAPENNGPSDLHKATNFFCMPLQEFTPDAGRYDVIWVQWCIGHLTDEDFISFF 180

Query: 181 KRAKLGLKAGGIFILKENIARSGFVLDKEDRSITRSDSYYKDLFNQCGLYIFKSRDQKGF 240
           KRAKLGLKAGGIFILKENIARSGF+LDKEDRSITRSDSYYKDLFNQCGLYIFKSRDQKGF
Sbjct: 181 KRAKLGLKAGGIFILKENIARSGFILDKEDRSITRSDSYYKDLFNQCGLYIFKSRDQKGF 240

Query: 241 PQELFPVKMYALTTEAPKRSSRIKREQSNRPGVIK 276
           PQELFPVKMYALTTE PKRSSRIKREQSNRPGVIK
Sbjct: 241 PQELFPVKMYALTTEVPKRSSRIKREQSNRPGVIK 275

BLAST of Chy2G023300 vs. ExPASy TrEMBL
Match: A0A1S3AYZ5 (alpha N-terminal protein methyltransferase 1 isoform X3 OS=Cucumis melo OX=3656 GN=LOC103484292 PE=3 SV=1)

HSP 1 Score: 562.4 bits (1448), Expect = 1.2e-156
Identity = 267/274 (97.45%), Postives = 271/274 (98.91%), Query Frame = 0

Query: 1   MEGSGADTDGHEFKNAEEMWREHVGNPTKRTEWYREGVGYWQGVEASVDGVLGGYGHVND 60
           MEGSGADTDGHEFKNAEEMWREHVGNPTKRTEWYREGVGYWQGVEASVDGVLGGYGHVND
Sbjct: 1   MEGSGADTDGHEFKNAEEMWREHVGNPTKRTEWYREGVGYWQGVEASVDGVLGGYGHVND 60

Query: 61  ADILGSEVFLKSILVERFSFAGKDRPLVALDCGSGIGRVTKNLLIKYFNEVDLLEPVSHF 120
           ADILGSEVFLKSIL ER SFAGKDRPLVALDCGSGIGR+TKNLLI+YFNEVDLLEPVSHF
Sbjct: 61  ADILGSEVFLKSILGERLSFAGKDRPLVALDCGSGIGRITKNLLIRYFNEVDLLEPVSHF 120

Query: 121 LEAARGNLAPENNGPSDLHKATNFFCMPLQEFTPDAGRYDVIWVQWCIGHLTDEDFISFF 180
           LEAARG+LAPENNGPSDLHKATNFFCMPLQEFTPDAGRYDVIWVQWCIGHLTDEDFISFF
Sbjct: 121 LEAARGSLAPENNGPSDLHKATNFFCMPLQEFTPDAGRYDVIWVQWCIGHLTDEDFISFF 180

Query: 181 KRAKLGLKAGGIFILKENIARSGFVLDKEDRSITRSDSYYKDLFNQCGLYIFKSRDQKGF 240
           KRAKLGLKAGGIFILKENIARSGF+LDKEDRSITRSDSYYKDLFNQCGLYIFKSRDQKGF
Sbjct: 181 KRAKLGLKAGGIFILKENIARSGFILDKEDRSITRSDSYYKDLFNQCGLYIFKSRDQKGF 240

Query: 241 PQELFPVKMYALTTEAPKRSSRIKREQSNRPGVI 275
           PQELFPVKMYALTTE PKRSSRIKREQSNRPGVI
Sbjct: 241 PQELFPVKMYALTTEVPKRSSRIKREQSNRPGVI 274

BLAST of Chy2G023300 vs. NCBI nr
Match: XP_011658332.1 (alpha N-terminal protein methyltransferase 1 isoform X1 [Cucumis sativus])

HSP 1 Score: 649 bits (1674), Expect = 2.91e-235
Identity = 312/314 (99.36%), Postives = 314/314 (100.00%), Query Frame = 0

Query: 1   MEGSGADTDGHEFKNAEEMWREHVGNPTKRTEWYREGVGYWQGVEASVDGVLGGYGHVND 60
           MEGSGADTDGHEFKNAEEMWREHVGNPTKRTEWYREGVGYWQGVEASVDGVLGGYGHVND
Sbjct: 1   MEGSGADTDGHEFKNAEEMWREHVGNPTKRTEWYREGVGYWQGVEASVDGVLGGYGHVND 60

Query: 61  ADILGSEVFLKSILVERFSFAGKDRPLVALDCGSGIGRVTKNLLIKYFNEVDLLEPVSHF 120
           ADILGSEVFLKSILVERFSFAGKDRPLVALDCGSGIGRVTKNLLIKYFNEVDLLEPVSHF
Sbjct: 61  ADILGSEVFLKSILVERFSFAGKDRPLVALDCGSGIGRVTKNLLIKYFNEVDLLEPVSHF 120

Query: 121 LEAARGNLAPENNGPSDLHKATNFFCMPLQEFTPDAGRYDVIWVQWCIGHLTDEDFISFF 180
           LEAARGNLAPENNGPSDLHKATNFFCMPLQEFTPDAGRYDVIWVQWCIGHLTDEDFISFF
Sbjct: 121 LEAARGNLAPENNGPSDLHKATNFFCMPLQEFTPDAGRYDVIWVQWCIGHLTDEDFISFF 180

Query: 181 KRAKLGLKAGGIFILKENIARSGFVLDKEDRSITRSDSYYKDLFNQCGLYIFKSRDQKGF 240
           KRAKLGLKAGGIFILKENIARSGFVLDKEDRSITRSDSYYKDLFNQCGLYIFKSRDQKGF
Sbjct: 181 KRAKLGLKAGGIFILKENIARSGFVLDKEDRSITRSDSYYKDLFNQCGLYIFKSRDQKGF 240

Query: 241 PQELFPVKMYALTTEAPKRSSRIKREQSNRPGVIKPVDIQIHSRWNWKHEASRFSYLQGD 300
           PQELFPVKMYALTTEAPKRSSRIKREQSNRPGVIKPVDIQIHSRWNWKH+ASRFSYLQGD
Sbjct: 241 PQELFPVKMYALTTEAPKRSSRIKREQSNRPGVIKPVDIQIHSRWNWKHQASRFSYLQGD 300

Query: 301 AIAIRPKALFPLLC 314
           AIAIRPKAL+PLLC
Sbjct: 301 AIAIRPKALYPLLC 314

BLAST of Chy2G023300 vs. NCBI nr
Match: XP_008439521.1 (PREDICTED: alpha N-terminal protein methyltransferase 1 isoform X1 [Cucumis melo])

HSP 1 Score: 638 bits (1646), Expect = 5.41e-231
Identity = 305/314 (97.13%), Postives = 310/314 (98.73%), Query Frame = 0

Query: 1   MEGSGADTDGHEFKNAEEMWREHVGNPTKRTEWYREGVGYWQGVEASVDGVLGGYGHVND 60
           MEGSGADTDGHEFKNAEEMWREHVGNPTKRTEWYREGVGYWQGVEASVDGVLGGYGHVND
Sbjct: 1   MEGSGADTDGHEFKNAEEMWREHVGNPTKRTEWYREGVGYWQGVEASVDGVLGGYGHVND 60

Query: 61  ADILGSEVFLKSILVERFSFAGKDRPLVALDCGSGIGRVTKNLLIKYFNEVDLLEPVSHF 120
           ADILGSEVFLKSIL ER SFAGKDRPLVALDCGSGIGR+TKNLLI+YFNEVDLLEPVSHF
Sbjct: 61  ADILGSEVFLKSILGERLSFAGKDRPLVALDCGSGIGRITKNLLIRYFNEVDLLEPVSHF 120

Query: 121 LEAARGNLAPENNGPSDLHKATNFFCMPLQEFTPDAGRYDVIWVQWCIGHLTDEDFISFF 180
           LEAARG+LAPENNGPSDLHKATNFFCMPLQEFTPDAGRYDVIWVQWCIGHLTDEDFISFF
Sbjct: 121 LEAARGSLAPENNGPSDLHKATNFFCMPLQEFTPDAGRYDVIWVQWCIGHLTDEDFISFF 180

Query: 181 KRAKLGLKAGGIFILKENIARSGFVLDKEDRSITRSDSYYKDLFNQCGLYIFKSRDQKGF 240
           KRAKLGLKAGGIFILKENIARSGF+LDKEDRSITRSDSYYKDLFNQCGLYIFKSRDQKGF
Sbjct: 181 KRAKLGLKAGGIFILKENIARSGFILDKEDRSITRSDSYYKDLFNQCGLYIFKSRDQKGF 240

Query: 241 PQELFPVKMYALTTEAPKRSSRIKREQSNRPGVIKPVDIQIHSRWNWKHEASRFSYLQGD 300
           PQELFPVKMYALTTE PKRSSRIKREQSNRPGVIKPVDIQIHSRWNWKH+ASRFSYLQGD
Sbjct: 241 PQELFPVKMYALTTEVPKRSSRIKREQSNRPGVIKPVDIQIHSRWNWKHQASRFSYLQGD 300

Query: 301 AIAIRPKALFPLLC 314
            IAIRPKALFPLLC
Sbjct: 301 VIAIRPKALFPLLC 314

BLAST of Chy2G023300 vs. NCBI nr
Match: XP_038881057.1 (alpha N-terminal protein methyltransferase 1 isoform X2 [Benincasa hispida])

HSP 1 Score: 608 bits (1568), Expect = 4.21e-219
Identity = 290/314 (92.36%), Postives = 301/314 (95.86%), Query Frame = 0

Query: 1   MEGSGADTDGHEFKNAEEMWREHVGNPTKRTEWYREGVGYWQGVEASVDGVLGGYGHVND 60
           MEGSGADTDGHEFKNAEEMW E VGNPTKRT+WYREGVGYWQGVEASVDGVLGGYGHVND
Sbjct: 1   MEGSGADTDGHEFKNAEEMWMEQVGNPTKRTQWYREGVGYWQGVEASVDGVLGGYGHVND 60

Query: 61  ADILGSEVFLKSILVERFSFAGKDRPLVALDCGSGIGRVTKNLLIKYFNEVDLLEPVSHF 120
           ADILGSEVFLKSIL ERF F GKDRPLVALDCGSGIGRVTKNLLI+YFNEVDLLEPVSHF
Sbjct: 61  ADILGSEVFLKSILAERFPFPGKDRPLVALDCGSGIGRVTKNLLIRYFNEVDLLEPVSHF 120

Query: 121 LEAARGNLAPENNGPSDLHKATNFFCMPLQEFTPDAGRYDVIWVQWCIGHLTDEDFISFF 180
           LEAARG+LAPENNG SDLHKATNFFCMPLQEFTPDAGRYDVIWVQWCIGHLTDEDFISFF
Sbjct: 121 LEAARGSLAPENNGASDLHKATNFFCMPLQEFTPDAGRYDVIWVQWCIGHLTDEDFISFF 180

Query: 181 KRAKLGLKAGGIFILKENIARSGFVLDKEDRSITRSDSYYKDLFNQCGLYIFKSRDQKGF 240
           KRAKLGLKAGGIFILKENIARSGF+LDKEDRSITRSD YYKDLFNQCGLY FKSRDQKGF
Sbjct: 181 KRAKLGLKAGGIFILKENIARSGFILDKEDRSITRSDPYYKDLFNQCGLYPFKSRDQKGF 240

Query: 241 PQELFPVKMYALTTEAPKRSSRIKREQSNRPGVIKPVDIQIHSRWNWKHEASRFSYLQGD 300
           PQELFPVKMYAL+TE PKRSSR KREQ+NRPGVIKPVDIQIHSRWNWKH+ASRFS LQG+
Sbjct: 241 PQELFPVKMYALSTEVPKRSSRTKREQNNRPGVIKPVDIQIHSRWNWKHQASRFSSLQGN 300

Query: 301 AIAIRPKALFPLLC 314
            +AIRP++ FPLLC
Sbjct: 301 VVAIRPQSPFPLLC 314

BLAST of Chy2G023300 vs. NCBI nr
Match: XP_038881056.1 (alpha N-terminal protein methyltransferase 1 isoform X1 [Benincasa hispida])

HSP 1 Score: 598 bits (1543), Expect = 4.58e-215
Identity = 290/328 (88.41%), Postives = 301/328 (91.77%), Query Frame = 0

Query: 1   MEGSGADTDGHEFKNAEEMWREHVGNPTKRTEWYREGVGYWQGVEASVDGVLGGYGHVND 60
           MEGSGADTDGHEFKNAEEMW E VGNPTKRT+WYREGVGYWQGVEASVDGVLGGYGHVND
Sbjct: 1   MEGSGADTDGHEFKNAEEMWMEQVGNPTKRTQWYREGVGYWQGVEASVDGVLGGYGHVND 60

Query: 61  ADILGSEVFLKSILVERFSFAGKDRPLVALDCGSGIGRVTKNLLIKYFNEVDLLEPVSHF 120
           ADILGSEVFLKSIL ERF F GKDRPLVALDCGSGIGRVTKNLLI+YFNEVDLLEPVSHF
Sbjct: 61  ADILGSEVFLKSILAERFPFPGKDRPLVALDCGSGIGRVTKNLLIRYFNEVDLLEPVSHF 120

Query: 121 LEAARGNLAPENNGPSDLHKATNFFCMPLQEFTPDAGRYDVIWVQWCIGHLTDEDFISFF 180
           LEAARG+LAPENNG SDLHKATNFFCMPLQEFTPDAGRYDVIWVQWCIGHLTDEDFISFF
Sbjct: 121 LEAARGSLAPENNGASDLHKATNFFCMPLQEFTPDAGRYDVIWVQWCIGHLTDEDFISFF 180

Query: 181 KRAK--------------LGLKAGGIFILKENIARSGFVLDKEDRSITRSDSYYKDLFNQ 240
           KRAK              LGLKAGGIFILKENIARSGF+LDKEDRSITRSD YYKDLFNQ
Sbjct: 181 KRAKVDYAYWLSPFSYFQLGLKAGGIFILKENIARSGFILDKEDRSITRSDPYYKDLFNQ 240

Query: 241 CGLYIFKSRDQKGFPQELFPVKMYALTTEAPKRSSRIKREQSNRPGVIKPVDIQIHSRWN 300
           CGLY FKSRDQKGFPQELFPVKMYAL+TE PKRSSR KREQ+NRPGVIKPVDIQIHSRWN
Sbjct: 241 CGLYPFKSRDQKGFPQELFPVKMYALSTEVPKRSSRTKREQNNRPGVIKPVDIQIHSRWN 300

Query: 301 WKHEASRFSYLQGDAIAIRPKALFPLLC 314
           WKH+ASRFS LQG+ +AIRP++ FPLLC
Sbjct: 301 WKHQASRFSSLQGNVVAIRPQSPFPLLC 328

BLAST of Chy2G023300 vs. NCBI nr
Match: XP_004134551.1 (alpha N-terminal protein methyltransferase 1 isoform X2 [Cucumis sativus] >KAE8647773.1 hypothetical protein Csa_002956 [Cucumis sativus])

HSP 1 Score: 566 bits (1460), Expect = 2.87e-203
Identity = 275/275 (100.00%), Postives = 275/275 (100.00%), Query Frame = 0

Query: 1   MEGSGADTDGHEFKNAEEMWREHVGNPTKRTEWYREGVGYWQGVEASVDGVLGGYGHVND 60
           MEGSGADTDGHEFKNAEEMWREHVGNPTKRTEWYREGVGYWQGVEASVDGVLGGYGHVND
Sbjct: 1   MEGSGADTDGHEFKNAEEMWREHVGNPTKRTEWYREGVGYWQGVEASVDGVLGGYGHVND 60

Query: 61  ADILGSEVFLKSILVERFSFAGKDRPLVALDCGSGIGRVTKNLLIKYFNEVDLLEPVSHF 120
           ADILGSEVFLKSILVERFSFAGKDRPLVALDCGSGIGRVTKNLLIKYFNEVDLLEPVSHF
Sbjct: 61  ADILGSEVFLKSILVERFSFAGKDRPLVALDCGSGIGRVTKNLLIKYFNEVDLLEPVSHF 120

Query: 121 LEAARGNLAPENNGPSDLHKATNFFCMPLQEFTPDAGRYDVIWVQWCIGHLTDEDFISFF 180
           LEAARGNLAPENNGPSDLHKATNFFCMPLQEFTPDAGRYDVIWVQWCIGHLTDEDFISFF
Sbjct: 121 LEAARGNLAPENNGPSDLHKATNFFCMPLQEFTPDAGRYDVIWVQWCIGHLTDEDFISFF 180

Query: 181 KRAKLGLKAGGIFILKENIARSGFVLDKEDRSITRSDSYYKDLFNQCGLYIFKSRDQKGF 240
           KRAKLGLKAGGIFILKENIARSGFVLDKEDRSITRSDSYYKDLFNQCGLYIFKSRDQKGF
Sbjct: 181 KRAKLGLKAGGIFILKENIARSGFVLDKEDRSITRSDSYYKDLFNQCGLYIFKSRDQKGF 240

Query: 241 PQELFPVKMYALTTEAPKRSSRIKREQSNRPGVIK 275
           PQELFPVKMYALTTEAPKRSSRIKREQSNRPGVIK
Sbjct: 241 PQELFPVKMYALTTEAPKRSSRIKREQSNRPGVIK 275

BLAST of Chy2G023300 vs. TAIR 10
Match: AT5G44450.1 (methyltransferases )

HSP 1 Score: 397.1 bits (1019), Expect = 1.3e-110
Identity = 188/279 (67.38%), Postives = 231/279 (82.80%), Query Frame = 0

Query: 1   MEGSGADTDGHEFKNAEEMWREHV--GNPTKRTEWYREGVGYWQGVEASVDGVLGGYGHV 60
           M+  G D++G EF + +EMWRE +  G+ TK+T+WYR+GV YW+GVEASVDGVLGGYGHV
Sbjct: 59  MDICGVDSEGKEFNSVQEMWREEIGEGDETKKTQWYRDGVSYWEGVEASVDGVLGGYGHV 118

Query: 61  NDADILGSEVFLKSILVERF-SFAGKDRPLVALDCGSGIGRVTKNLLIKYFNEVDLLEPV 120
           NDADI+GSEVFLK++L ER  +  G ++ LVALDCGSGIGR+TKNLLI+YFNEVDLLEPV
Sbjct: 119 NDADIIGSEVFLKTLLQERLVNNVGANQHLVALDCGSGIGRITKNLLIRYFNEVDLLEPV 178

Query: 121 SHFLEAARGNLAPENNGPSDLHKATNFFCMPLQEFTPDAGRYDVIWVQWCIGHLTDEDFI 180
           + FL+AAR NLA   +  S+ HKATNFFC+PLQEFTP AGRYDVIWVQWCIGHLTD DF+
Sbjct: 179 AQFLDAARENLA---SAGSETHKATNFFCVPLQEFTPAAGRYDVIWVQWCIGHLTDNDFV 238

Query: 181 SFFKRAKLGLKAGGIFILKENIARSGFVLDKEDRSITRSDSYYKDLFNQCGLYIFKSRDQ 240
           SFF RAK  LK GG F++KEN+A++GFVLDKED SITRSD Y+K LF QCGL++++++DQ
Sbjct: 239 SFFNRAKGYLKPGGFFVVKENLAKNGFVLDKEDHSITRSDPYFKQLFRQCGLHLYRTKDQ 298

Query: 241 KGFPQELFPVKMYALTTEAPKRSSRIK-REQSNRPGVIK 276
           KG PQELF VKMYALT + P +  R + + +SNRP +IK
Sbjct: 299 KGLPQELFAVKMYALTVDTPPKIHRTRSKTRSNRPQIIK 334

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q5PP701.8e-10967.38Alpha N-terminal protein methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q10CT53.3e-9556.49Alpha N-terminal protein methyltransferase 1 OS=Oryza sativa subsp. japonica OX=... [more]
A2XMJ15.7e-9556.17Alpha N-terminal protein methyltransferase 1 OS=Oryza sativa subsp. indica OX=39... [more]
Q55DH61.6e-4940.47Alpha N-terminal protein methyltransferase 1 OS=Dictyostelium discoideum OX=4468... [more]
Q4KLE62.8e-4941.44N-terminal Xaa-Pro-Lys N-methyltransferase 1-B OS=Xenopus laevis OX=8355 GN=ntmt... [more]
Match NameE-valueIdentityDescription
A0A0A0KIY92.1e-18599.36Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G525730 PE=3 SV=1[more]
A0A1S3AZL93.7e-18297.13alpha N-terminal protein methyltransferase 1 isoform X1 OS=Cucumis melo OX=3656 ... [more]
A0A6J1CY141.4e-15785.71alpha N-terminal protein methyltransferase 1 isoform X1 OS=Momordica charantia O... [more]
A0A1S3AYJ53.2e-15797.45alpha N-terminal protein methyltransferase 1 isoform X2 OS=Cucumis melo OX=3656 ... [more]
A0A1S3AYZ51.2e-15697.45alpha N-terminal protein methyltransferase 1 isoform X3 OS=Cucumis melo OX=3656 ... [more]
Match NameE-valueIdentityDescription
XP_011658332.12.91e-23599.36alpha N-terminal protein methyltransferase 1 isoform X1 [Cucumis sativus][more]
XP_008439521.15.41e-23197.13PREDICTED: alpha N-terminal protein methyltransferase 1 isoform X1 [Cucumis melo... [more]
XP_038881057.14.21e-21992.36alpha N-terminal protein methyltransferase 1 isoform X2 [Benincasa hispida][more]
XP_038881056.14.58e-21588.41alpha N-terminal protein methyltransferase 1 isoform X1 [Benincasa hispida][more]
XP_004134551.12.87e-203100.00alpha N-terminal protein methyltransferase 1 isoform X2 [Cucumis sativus] >KAE86... [more]
Match NameE-valueIdentityDescription
AT5G44450.11.3e-11067.38methyltransferases [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008576Alpha-N-methyltransferase NTM1PFAMPF05891Methyltransf_PKcoord: 30..253
e-value: 1.1E-92
score: 309.5
IPR008576Alpha-N-methyltransferase NTM1PANTHERPTHR12753AD-003 - RELATEDcoord: 1..275
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 25..253
e-value: 7.7E-84
score: 282.5
NoneNo IPR availablePIRSRPIRSR016958-1PIRSR016958-1coord: 33..253
e-value: 5.0E-72
score: 239.4
NoneNo IPR availablePANTHERPTHR12753:SF4BNACNNG52650D PROTEINcoord: 1..275
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 90..196
e-value: 1.38975E-6
score: 44.3431
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 31..252

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy2G023300.1Chy2G023300.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006480 N-terminal protein amino acid methylation
cellular_component GO:0005737 cytoplasm
molecular_function GO:0008168 methyltransferase activity