Chy1G021200 (gene) Cucumber (hystrix) v1

Overview
NameChy1G021200
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
Description(R)-mandelonitrile lyase-like
LocationchrH01: 25875271 .. 25877068 (-)
RNA-Seq ExpressionChy1G021200
SyntenyChy1G021200
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCTCTGTTTCCTTCTTCCAAGAAACACTCACAAGCTTTCCTCTCTCCATTAATGGCGTCTCGCATTTTCTTCCACTCTCAAATCCTCACGTTCTTGCTCATCTTTTCCGCTTCATCCGCTCTTCCAAACTCCTCTCAACAAGGTATAATTTAAACAATTTTTTTTAAAAAAATTATTGAATATAATGAATTAATAATCTGAAATTTACGTGAAACCAGATCCCGATTACCTGAAATTTGTGTCCAATGCGACGGAATTTCCCGAAGTAGATTACTACGACTACATAATCATCGGCGGCGGAACGGCCGGTTGTCCACTCGCTGCCACATTATCAAGCAAATTCCGAGTTCTTGTTCTTGAACGCGGCGGCGTTCCTCACGGCAACTCCAATCTGATGTCTCAGGAAGGATTCTTGACGACTCTAATGGACGACGTTCACTCACGTAACTCCCCCGTGCAAGCATTCACCTCAGAAGAAGGAGTTCCCAATGCCAGAGGACGCATCCTCGGTGGCAGTAGCGCCATTAACGCTGGCTTCTACAGCCGAGCCGATCAAGCCTTCTTCAAAAACTCACAACTCAAATGGGACCTCGCCATCGTCAATCAATCCTACGAATGGGTTGAAAAGGAAATCGTGTTCAAACCAAATCTCAAGAACTGGCAATCCGCGGTTCGCGACGGAATGGTCGAAGCTGGAATAATTCCGTACAACGGCTTCAGTTTAGATCACGTAATGGGAACCAAAATCGGGGGCTCTACATTCGACGAGTTAGGCCGACGTCATAGCGCCGCCGATCTTCTCTCTTATGCGACGCCGTTCAATATCAAAGTCGCCATCTACGCCACCGTTGAGAGAATTCTGTTAGCTTCCACAGTCAAACGAAAACCAAAAGCAATTGGTGTTCTTTATCGTGACCAAATCGGCACGTATCATCACGCTATGCTACACGCACGTGGAGAAGTATTCTTATCAGCAGGCGCAATCGGAAGTCCGCAATTACTGTTACTCAGTGGAATCGGATCGCGTCCATATCTCTCATCTTGGGGCATTCCAGTAGCTCATCATTTACCCTACGTCGGCCATTACCTCTACGACAACCCTAGAAACGGAATTTCCATTTTAGCGCCGATGCCATTGGAGCAATCTCTAATTCAAGTAGCCGGAATTACAGAATCAGGAACATACCTTGAAGCAGCATCAAATGTAATCCCGTTCACCTCGCCGCCGAGGTCATTTTTCATTAGAACACCATCGCCGCCGGTGTATCTGACGGTGGCGACACTAATGGAAAAAATAACCGGACCGGTTGCCTCCGGCTCGATGCGACTGGCGTCGACGGACGTAAGGGTGAATCCGGTGGTGCGATTCAATTACTTCGGAAACGCGGTGGACCTGGAGAGGTGTGTGAACGGGACAAGGAAGATTGGGGAGATTCTACGGAGCCAGGCGATGGAGGGGTTCAAATTCAGGGATTGGTTCGGGAGGAAGGAATTCCGGTACGTTGGGCCGGCTTATCCGGAGAAAGAAGATGAAGATGGGTTGGTGTTGAGGGAATTCTGCCGGCAGAGTGTGAGCACGATATGGCACTACCATGGAGGATGTGTGGCGGGGAAAGTGGTGGATTCTAATTATCGGGTTATTGGAATGGAAGCGCTGAGAGTTGTGGATGGCTCGATTTTTGGGGTTTCGCCGGGTACTAATCCTCAAGCTACGGTTATGATGCTTGGACGACATGTCGGTTTGCAAATTATCAACCAACGCTCTGCTGTTACGACGCATCGTGTAGTATATTAG

mRNA sequence

ATGCCTCTGTTTCCTTCTTCCAAGAAACACTCACAAGCTTTCCTCTCTCCATTAATGGCGTCTCGCATTTTCTTCCACTCTCAAATCCTCACGTTCTTGCTCATCTTTTCCGCTTCATCCGCTCTTCCAAACTCCTCTCAACAAGATCCCGATTACCTGAAATTTGTGTCCAATGCGACGGAATTTCCCGAAGTAGATTACTACGACTACATAATCATCGGCGGCGGAACGGCCGGTTGTCCACTCGCTGCCACATTATCAAGCAAATTCCGAGTTCTTGTTCTTGAACGCGGCGGCGTTCCTCACGGCAACTCCAATCTGATGTCTCAGGAAGGATTCTTGACGACTCTAATGGACGACGTTCACTCACGTAACTCCCCCGTGCAAGCATTCACCTCAGAAGAAGGAGTTCCCAATGCCAGAGGACGCATCCTCGGTGGCAGTAGCGCCATTAACGCTGGCTTCTACAGCCGAGCCGATCAAGCCTTCTTCAAAAACTCACAACTCAAATGGGACCTCGCCATCGTCAATCAATCCTACGAATGGGTTGAAAAGGAAATCGTGTTCAAACCAAATCTCAAGAACTGGCAATCCGCGGTTCGCGACGGAATGGTCGAAGCTGGAATAATTCCGTACAACGGCTTCAGTTTAGATCACGTAATGGGAACCAAAATCGGGGGCTCTACATTCGACGAGTTAGGCCGACGTCATAGCGCCGCCGATCTTCTCTCTTATGCGACGCCGTTCAATATCAAAGTCGCCATCTACGCCACCGTTGAGAGAATTCTGTTAGCTTCCACAGTCAAACGAAAACCAAAAGCAATTGGTGTTCTTTATCGTGACCAAATCGGCACGTATCATCACGCTATGCTACACGCACGTGGAGAAGTATTCTTATCAGCAGGCGCAATCGGAAGTCCGCAATTACTGTTACTCAGTGGAATCGGATCGCGTCCATATCTCTCATCTTGGGGCATTCCAGTAGCTCATCATTTACCCTACGTCGGCCATTACCTCTACGACAACCCTAGAAACGGAATTTCCATTTTAGCGCCGATGCCATTGGAGCAATCTCTAATTCAAGTAGCCGGAATTACAGAATCAGGAACATACCTTGAAGCAGCATCAAATGTAATCCCGTTCACCTCGCCGCCGAGGTCATTTTTCATTAGAACACCATCGCCGCCGGTGTATCTGACGGTGGCGACACTAATGGAAAAAATAACCGGACCGGTTGCCTCCGGCTCGATGCGACTGGCGTCGACGGACGTAAGGGTGAATCCGGTGGTGCGATTCAATTACTTCGGAAACGCGGTGGACCTGGAGAGGTGTGTGAACGGGACAAGGAAGATTGGGGAGATTCTACGGAGCCAGGCGATGGAGGGGTTCAAATTCAGGGATTGGTTCGGGAGGAAGGAATTCCGGTACGTTGGGCCGGCTTATCCGGAGAAAGAAGATGAAGATGGGTTGGTGTTGAGGGAATTCTGCCGGCAGAGTGTGAGCACGATATGGCACTACCATGGAGGATGTGTGGCGGGGAAAGTGGTGGATTCTAATTATCGGGTTATTGGAATGGAAGCGCTGAGAGTTGTGGATGGCTCGATTTTTGGGGTTTCGCCGGGTACTAATCCTCAAGCTACGGTTATGATGCTTGGACGACATGTCGGTTTGCAAATTATCAACCAACGCTCTGCTGTTACGACGCATCGTGTAGTATATTAG

Coding sequence (CDS)

ATGCCTCTGTTTCCTTCTTCCAAGAAACACTCACAAGCTTTCCTCTCTCCATTAATGGCGTCTCGCATTTTCTTCCACTCTCAAATCCTCACGTTCTTGCTCATCTTTTCCGCTTCATCCGCTCTTCCAAACTCCTCTCAACAAGATCCCGATTACCTGAAATTTGTGTCCAATGCGACGGAATTTCCCGAAGTAGATTACTACGACTACATAATCATCGGCGGCGGAACGGCCGGTTGTCCACTCGCTGCCACATTATCAAGCAAATTCCGAGTTCTTGTTCTTGAACGCGGCGGCGTTCCTCACGGCAACTCCAATCTGATGTCTCAGGAAGGATTCTTGACGACTCTAATGGACGACGTTCACTCACGTAACTCCCCCGTGCAAGCATTCACCTCAGAAGAAGGAGTTCCCAATGCCAGAGGACGCATCCTCGGTGGCAGTAGCGCCATTAACGCTGGCTTCTACAGCCGAGCCGATCAAGCCTTCTTCAAAAACTCACAACTCAAATGGGACCTCGCCATCGTCAATCAATCCTACGAATGGGTTGAAAAGGAAATCGTGTTCAAACCAAATCTCAAGAACTGGCAATCCGCGGTTCGCGACGGAATGGTCGAAGCTGGAATAATTCCGTACAACGGCTTCAGTTTAGATCACGTAATGGGAACCAAAATCGGGGGCTCTACATTCGACGAGTTAGGCCGACGTCATAGCGCCGCCGATCTTCTCTCTTATGCGACGCCGTTCAATATCAAAGTCGCCATCTACGCCACCGTTGAGAGAATTCTGTTAGCTTCCACAGTCAAACGAAAACCAAAAGCAATTGGTGTTCTTTATCGTGACCAAATCGGCACGTATCATCACGCTATGCTACACGCACGTGGAGAAGTATTCTTATCAGCAGGCGCAATCGGAAGTCCGCAATTACTGTTACTCAGTGGAATCGGATCGCGTCCATATCTCTCATCTTGGGGCATTCCAGTAGCTCATCATTTACCCTACGTCGGCCATTACCTCTACGACAACCCTAGAAACGGAATTTCCATTTTAGCGCCGATGCCATTGGAGCAATCTCTAATTCAAGTAGCCGGAATTACAGAATCAGGAACATACCTTGAAGCAGCATCAAATGTAATCCCGTTCACCTCGCCGCCGAGGTCATTTTTCATTAGAACACCATCGCCGCCGGTGTATCTGACGGTGGCGACACTAATGGAAAAAATAACCGGACCGGTTGCCTCCGGCTCGATGCGACTGGCGTCGACGGACGTAAGGGTGAATCCGGTGGTGCGATTCAATTACTTCGGAAACGCGGTGGACCTGGAGAGGTGTGTGAACGGGACAAGGAAGATTGGGGAGATTCTACGGAGCCAGGCGATGGAGGGGTTCAAATTCAGGGATTGGTTCGGGAGGAAGGAATTCCGGTACGTTGGGCCGGCTTATCCGGAGAAAGAAGATGAAGATGGGTTGGTGTTGAGGGAATTCTGCCGGCAGAGTGTGAGCACGATATGGCACTACCATGGAGGATGTGTGGCGGGGAAAGTGGTGGATTCTAATTATCGGGTTATTGGAATGGAAGCGCTGAGAGTTGTGGATGGCTCGATTTTTGGGGTTTCGCCGGGTACTAATCCTCAAGCTACGGTTATGATGCTTGGACGACATGTCGGTTTGCAAATTATCAACCAACGCTCTGCTGTTACGACGCATCGTGTAGTATATTAG

Protein sequence

MPLFPSSKKHSQAFLSPLMASRIFFHSQILTFLLIFSASSALPNSSQQDPDYLKFVSNATEFPEVDYYDYIIIGGGTAGCPLAATLSSKFRVLVLERGGVPHGNSNLMSQEGFLTTLMDDVHSRNSPVQAFTSEEGVPNARGRILGGSSAINAGFYSRADQAFFKNSQLKWDLAIVNQSYEWVEKEIVFKPNLKNWQSAVRDGMVEAGIIPYNGFSLDHVMGTKIGGSTFDELGRRHSAADLLSYATPFNIKVAIYATVERILLASTVKRKPKAIGVLYRDQIGTYHHAMLHARGEVFLSAGAIGSPQLLLLSGIGSRPYLSSWGIPVAHHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITESGTYLEAASNVIPFTSPPRSFFIRTPSPPVYLTVATLMEKITGPVASGSMRLASTDVRVNPVVRFNYFGNAVDLERCVNGTRKIGEILRSQAMEGFKFRDWFGRKEFRYVGPAYPEKEDEDGLVLREFCRQSVSTIWHYHGGCVAGKVVDSNYRVIGMEALRVVDGSIFGVSPGTNPQATVMMLGRHVGLQIINQRSAVTTHRVVY*
Homology
BLAST of Chy1G021200 vs. ExPASy Swiss-Prot
Match: Q9SSM2 ((R)-mandelonitrile lyase-like OS=Arabidopsis thaliana OX=3702 GN=At1g73050 PE=2 SV=1)

HSP 1 Score: 724.9 bits (1870), Expect = 7.0e-208
Identity = 349/538 (64.87%), Postives = 435/538 (80.86%), Query Frame = 0

Query: 29  ILTFLLIFSASSALPNSSQQDPDYLKFVSNATEFPEVDYYDYIIIGGGTAGCPLAATLSS 88
           +L  L +   S+A P  + + P +++F+SNAT+F   DYYDYII+GGGTAGCPLAATLS 
Sbjct: 16  VLLLLGVVHRSNARPRVN-RPPGFMRFISNATDFASEDYYDYIIVGGGTAGCPLAATLSQ 75

Query: 89  KFRVLVLERGGVPHGNSNLMSQEGFLTTLMDDVHSRNSPVQAFTSEEGVPNARGRILGGS 148
            FRVL+LERGGVP+   N+MS +GFLTTL  DV++ +SP Q+F SEEGVPNARGR+LGGS
Sbjct: 76  SFRVLLLERGGVPYNRPNVMSHDGFLTTL-TDVNNFDSPAQSFISEEGVPNARGRVLGGS 135

Query: 149 SAINAGFYSRADQAFFKNSQLKWDLAIVNQSYEWVEKEIVFKPNLKNWQSAVRDGMVEAG 208
           SAINAGFYSRAD+ FF+NS L WDL+ VNQSYEWVE+ IVF+P L+ WQ+A+RD ++E G
Sbjct: 136 SAINAGFYSRADKQFFENSGLVWDLSSVNQSYEWVERAIVFRPQLRTWQTAIRDALLEVG 195

Query: 209 IIPYNGFSLDHVMGTKIGGSTFDELGRRHSAADLLSYATPFNIKVAIYATVERILLASTV 268
           + P+NGF+L+H +GTKIGGSTFD  GRRHS+ADLL YA   NI+VA+YATVER+LLAS+ 
Sbjct: 196 VHPFNGFTLEHKVGTKIGGSTFDRTGRRHSSADLLRYARSSNIRVAVYATVERVLLASSP 255

Query: 269 K---RKPKAIGVLYRDQIGTYHHAMLHARGEVFLSAGAIGSPQLLLLSGIGSRPYLSSWG 328
                   AIGV+YRDQ+G +HHA++  RGEV LSAGA+GSPQLL LSGIG R YLS+WG
Sbjct: 256 SVSGSNVSAIGVVYRDQLGRFHHALIRDRGEVILSAGALGSPQLLFLSGIGPRSYLSTWG 315

Query: 329 IPVAHHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITESGTYLEAASNVIPFTSPP 388
           IPVA   P+VG ++YDNPRNGISI+ P+P+E SLIQV G+TE G +LEAASNVIPF SP 
Sbjct: 316 IPVALDQPHVGDFVYDNPRNGISIVPPVPMENSLIQVVGVTEDGAFLEAASNVIPFASPL 375

Query: 389 RSFFIRTPSPPVYLTVATLMEKITGPVASGSMRLASTDVRVNPVVRFNYFGNAVDLERCV 448
            S FIR P+ P+Y+ V T+MEKI GPV+ G +RLASTDVR+NPVVRFNYF +  DLERCV
Sbjct: 376 HSVFIRAPASPLYVPVTTIMEKILGPVSIGLLRLASTDVRINPVVRFNYFSDPQDLERCV 435

Query: 449 NGTRKIGEILRSQAMEGFKFRDWFGRKEFRYVGPAYPEKEDEDGLVLREFCRQSVSTIWH 508
           NGTRKIGEILRS+AM+ F  R+WFG + FR+VG   P  +  D LV+ +FCR++VSTIWH
Sbjct: 436 NGTRKIGEILRSRAMQDFMIREWFGNRRFRFVGAPLPVDQSND-LVMADFCRRTVSTIWH 495

Query: 509 YHGGCVAGKVVDSNYRVIGMEALRVVDGSIFGVSPGTNPQATVMMLGRHVGLQIINQR 564
           YHGG V GKVVDS+ +VIG+ +LR+VDGS F +SPGTNPQAT+MMLGR++GL+++ +R
Sbjct: 496 YHGGAVVGKVVDSDLKVIGVNSLRLVDGSTFNISPGTNPQATLMMLGRYMGLKMLRER 550

BLAST of Chy1G021200 vs. ExPASy Swiss-Prot
Match: P52707 ((R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1)

HSP 1 Score: 515.8 bits (1327), Expect = 6.4e-145
Identity = 262/547 (47.90%), Postives = 365/547 (66.73%), Query Frame = 0

Query: 21  SRIFFHSQILTFLLIFSASSALPNSSQQDPDYLKFVSNATEFPEVD-YYDYIIIGGGTAG 80
           S +     I    L +S   +L N+S  D  YL FV +AT+ PE++  YDYII+GGGTAG
Sbjct: 7   SAVLLVLHIFVLHLQYSEVQSLANTSSHDFSYLSFVYDATD-PELEGSYDYIIVGGGTAG 66

Query: 81  CPLAATLSSKFRVLVLERGGVPHGNSNLMSQEGFLTTLMDDVHSRNSPVQAFTSEEGVPN 140
           CPLAATLS+ + VLVLERG +P    NL+  +GF+  L  +   + +PV+ F SE+G+ N
Sbjct: 67  CPLAATLSANYSVLVLERGSLPTEYPNLLISDGFVYNLQQEDDGK-TPVERFVSEDGIDN 126

Query: 141 ARGRILGGSSAINAGFYSRADQAFFKNSQLKWDLAIVNQSYEWVEKEIVFKPNLKNWQSA 200
            RGR+LGG+S INAG Y RA+ +FF  + ++WD+ +VNQ+YEWVE  IVF+P+ + WQ+ 
Sbjct: 127 VRGRVLGGTSMINAGVYVRANTSFFNQTGIEWDMDLVNQTYEWVEDTIVFEPDSQTWQTV 186

Query: 201 VRDGMVEAGIIPYNGFSLDHVMGTKIGGSTFDELGRRHSAADLLSYATPFNIKVAIYATV 260
           +    +EAGI+P NGFS+DH+ GT++ GSTFD  G RH++ +LL+   P N++VA+ A V
Sbjct: 187 IGTAYLEAGILPNNGFSVDHLAGTRLTGSTFDNNGTRHASDELLNKGDPNNLRVAVQAAV 246

Query: 261 ERILLASTVKRKPKAIGVLYRDQIGTYHHAMLHARGEVFLSAGAIGSPQLLLLSGIGSRP 320
           E+I+ +S       AIGV+Y D  GT H A +   GEV LSAG IGSPQLLLLSG+G   
Sbjct: 247 EKIIFSSNTS-GVTAIGVIYTDSNGTTHQAFVRGEGEVILSAGPIGSPQLLLLSGVGPES 306

Query: 321 YLSSWGIPVAHHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITESGTYLEAASNVI 380
           YL+S  I V    PYVG Y+YDNPRN I+IL P P+E S + V GIT    + + + + +
Sbjct: 307 YLTSLNISVVASHPYVGQYIYDNPRNFINILPPNPIEASTVTVLGITSD--FYQCSISSL 366

Query: 381 PFTSPPRSFFIRTPSPPVYLTVATLMEKITGPVASGSMRL-ASTDVRVNPVVRFNYFGNA 440
           PF +PP SFF  T  P    T A ++ K+ GP++ G++ L +S+DVRV P V+FNY+ N 
Sbjct: 367 PFDTPPFSFFPTTSYPLPNQTFAHIVNKVPGPLSHGTVTLNSSSDVRVGPNVKFNYYSNL 426

Query: 441 VDLERCVNGTRKIGEILRSQAMEGFKFRDWFGRKEFRYVGPAYPEKEDEDGLVLREFCRQ 500
            DL  CV+G +K+GE+L + A+E +K  D  G   F  +G   PE + +D      FCR+
Sbjct: 427 TDLSHCVSGMKKLGEVLSTDALEPYKVEDLPGIDGFNILGIPLPENQTDDA-AFETFCRE 486

Query: 501 SVSTIWHYHGGCVAGKVVDSNYRVIGMEALRVVDGSIFGVSPGTNPQATVMMLGRHVGLQ 560
           SV++ WHYHGGC+ GKV+D  +RV G+ ALRVVDGS F  +P ++PQ   +MLGR++G+Q
Sbjct: 487 SVASYWHYHGGCLVGKVLDDGFRVTGINALRVVDGSTFPSTPASHPQGFYLMLGRYMGIQ 546

Query: 561 IINQRSA 566
           I+ +RSA
Sbjct: 547 ILQERSA 547

BLAST of Chy1G021200 vs. ExPASy Swiss-Prot
Match: P52706 ((R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1)

HSP 1 Score: 508.4 bits (1308), Expect = 1.0e-142
Identity = 255/546 (46.70%), Postives = 365/546 (66.85%), Query Frame = 0

Query: 21  SRIFFHSQILTFLLIFSASSALPNSSQQDPDYLKFVSNATEFPEVDYYDYIIIGGGTAGC 80
           S I     +   LL +S   +L  +S  D  YL+F  +AT+      YDY+I+GGGT+GC
Sbjct: 7   SAILLVLHLFVLLLQYSEVHSLATTSNHDFSYLRFAYDATDLELEGSYDYVIVGGGTSGC 66

Query: 81  PLAATLSSKFRVLVLERGGVPHGNSNLMSQEGFLTTLMDDVHSRNSPVQAFTSEEGVPNA 140
           PLAATLS K++VLVLERG +P    N+++ +GF+  L  +   + +PV+ F SE+G+ N 
Sbjct: 67  PLAATLSEKYKVLVLERGSLPTAYPNVLTADGFVYNLQQEDDGK-TPVERFVSEDGIDNV 126

Query: 141 RGRILGGSSAINAGFYSRADQAFFKNSQLKWDLAIVNQSYEWVEKEIVFKPNLKNWQSAV 200
           RGR+LGG+S INAG Y+RA+ + +  S + WD+ +VN++YEWVE  IVFKPN + WQS  
Sbjct: 127 RGRVLGGTSMINAGVYARANTSIYSASGVDWDMDLVNKTYEWVEDTIVFKPNYQPWQSVT 186

Query: 201 RDGMVEAGIIPYNGFSLDHVMGTKIGGSTFDELGRRHSAADLLSYATPFNIKVAIYATVE 260
               +EAG+ P +GFSLDH  GT+I GSTFD  G RH+A +LL+     N++V ++A+VE
Sbjct: 187 GTAFLEAGVDPNHGFSLDHEAGTRITGSTFDNKGTRHAADELLNKGNSNNLRVGVHASVE 246

Query: 261 RILLASTVKRKPKAIGVLYRDQIGTYHHAMLHARGEVFLSAGAIGSPQLLLLSGIGSRPY 320
           +I+ ++       A GV+YRD  GT H A + ++GEV +SAG IG+PQLLLLSG+G   Y
Sbjct: 247 KIIFSNAPGL--TATGVIYRDSNGTPHRAFVRSKGEVIVSAGTIGTPQLLLLSGVGPESY 306

Query: 321 LSSWGIPVAHHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITESGTYLEAASNVIP 380
           LSS  IPV    PYVG +L+DNPRN I+IL P P+E +++ V GI  S  + + + + +P
Sbjct: 307 LSSLNIPVVLSHPYVGQFLHDNPRNFINILPPNPIEPTIVTVLGI--SNDFYQCSFSSLP 366

Query: 381 FTSPPRSFFIRTPSPPVYLTVATLMEKITGPVASGSMRL-ASTDVRVNPVVRFNYFGNAV 440
           FT+PP SFF  T  P    T A    K+ GP++ GS+ L +S++VRV+P V+FNY+ N  
Sbjct: 367 FTTPPFSFFPSTSYPLPNSTFAHFASKVAGPLSYGSLTLKSSSNVRVSPNVKFNYYSNPT 426

Query: 441 DLERCVNGTRKIGEILRSQAMEGFKFRDWFGRKEFRYVGPAYPEKEDEDGLVLREFCRQS 500
           DL  CV+G +KIGE+L + A++ +K  D  G + F  +G   P K+  D      FCR+S
Sbjct: 427 DLSHCVSGMKKIGELLSTDALKPYKVEDLPGIEGFNILGIPLP-KDQTDDAAFETFCRES 486

Query: 501 VSTIWHYHGGCVAGKVVDSNYRVIGMEALRVVDGSIFGVSPGTNPQATVMMLGRHVGLQI 560
           V++ WHYHGGC+ GKV+D ++RV G++ALRVVDGS F  +P ++PQ   +MLGR+VG++I
Sbjct: 487 VASYWHYHGGCLVGKVLDGDFRVTGIDALRVVDGSTFPYTPASHPQGFYLMLGRYVGIKI 546

Query: 561 INQRSA 566
           + +RSA
Sbjct: 547 LQERSA 546

BLAST of Chy1G021200 vs. ExPASy Swiss-Prot
Match: O24243 ((R)-mandelonitrile lyase 1 OS=Prunus dulcis OX=3755 GN=MDL1 PE=1 SV=1)

HSP 1 Score: 503.8 bits (1296), Expect = 2.5e-141
Identity = 256/545 (46.97%), Postives = 362/545 (66.42%), Query Frame = 0

Query: 21  SRIFFHSQILTFLLIFSASSALPNSSQQDPDYLKFVSNATEFPEVDYYDYIIIGGGTAGC 80
           S I F   +L   L +S   +L N+S  D  YLKFV NAT+      YDYI+IGGGT+GC
Sbjct: 7   SVILFVLHLLVLHLQYSEVHSLANTSAHDFSYLKFVYNATDTSLEGSYDYIVIGGGTSGC 66

Query: 81  PLAATLSSKFRVLVLERGGVPHGNSNLMSQEGFLTTLMDDVHSRNSPVQAFTSEEGVPNA 140
           PLAATLS K++VL+LERG +     N ++ +GF   L      + +PV+ F SE+G+ N 
Sbjct: 67  PLAATLSEKYKVLLLERGTIATEYPNTLTADGFAYNLQQQDDGK-TPVERFVSEDGIDNV 126

Query: 141 RGRILGGSSAINAGFYSRADQAFFKNSQLKWDLAIVNQSYEWVEKEIVFKPNLKNWQSAV 200
           R RILGG++ INAG Y+RA+ +F+  + ++WDL +VN++YEWVE  IV KPN ++WQS +
Sbjct: 127 RARILGGTTIINAGVYARANISFYSQTGIEWDLDLVNKTYEWVEDAIVVKPNNQSWQSVI 186

Query: 201 RDGMVEAGIIPYNGFSLDHVMGTKIGGSTFDELGRRHSAADLLSYATPFNIKVAIYATVE 260
            +G +EAGI+P NGFSLDH  GT++ GSTFD  G RH+A +LL+   P N+ VA+ A+VE
Sbjct: 187 GEGFLEAGILPDNGFSLDHEAGTRLTGSTFDNNGTRHAADELLNKGDPNNLLVAVQASVE 246

Query: 261 RILLASTVKRKPKAIGVLYRDQIGTYHHAMLHARGEVFLSAGAIGSPQLLLLSGIGSRPY 320
           +IL +S       AIGV+Y D  G  H A +   GEV +SAG IG+PQLLLLSG+G   Y
Sbjct: 247 KILFSSNTSNL-SAIGVIYTDSDGNSHQAFVRGNGEVIVSAGTIGTPQLLLLSGVGPESY 306

Query: 321 LSSWGIPVAHHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITESGTYLEAASNVIP 380
           LSS  I V    PYVG +LY+NPRN I+   P P+E S++ V GI     Y + + + +P
Sbjct: 307 LSSLNITVVQPNPYVGQFLYNNPRNFINNFPPNPIEASVVTVLGIRSD--YYQVSLSSLP 366

Query: 381 FTSPPRSFFIRTPSPPVYLTVATLMEKITGPVASGSMRL-ASTDVRVNPVVRFNYFGNAV 440
           F++PP S F  T  P    T A ++ ++ GP++ GS+ L +S+DVR+ P ++FNY+ N+ 
Sbjct: 367 FSTPPFSLFPTTSYPLPNSTFAHIVSQVPGPLSHGSVTLNSSSDVRIAPNIKFNYYSNST 426

Query: 441 DLERCVNGTRKIGEILRSQAMEGFKFRDWFGRKEFRYVGPAYPEKEDEDGLVLREFCRQS 500
           DL  CV+G +K+G++LR++A+E +K RD  G   F Y+G   PE + +D      FC  +
Sbjct: 427 DLANCVSGMKKLGDLLRTKALEPYKARDVLGIDGFNYLGVPLPENQTDDA-SFETFCLDN 486

Query: 501 VSTIWHYHGGCVAGKVVDSNYRVIGMEALRVVDGSIFGVSPGTNPQATVMMLGRHVGLQI 560
           V++ WHYHGG + GKV+D ++RV+G++ALRVVD S F   P ++PQ   +MLGR+VGLQI
Sbjct: 487 VASYWHYHGGSLVGKVLDDSFRVMGIKALRVVDASTFPYEPNSHPQGFYLMLGRYVGLQI 546

Query: 561 INQRS 565
           + +RS
Sbjct: 547 LQERS 546

BLAST of Chy1G021200 vs. ExPASy Swiss-Prot
Match: Q945K2 ((R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1)

HSP 1 Score: 503.4 bits (1295), Expect = 3.3e-141
Identity = 253/546 (46.34%), Postives = 362/546 (66.30%), Query Frame = 0

Query: 21  SRIFFHSQILTFLLIFSASSALPNSSQQDPDYLKFVSNATEFPEVDYYDYIIIGGGTAGC 80
           S I     I    L +S   +L  +S  D  YL F  +AT+      YDY+I+GGGT+GC
Sbjct: 7   SAILLVLYIFVLHLQYSEVHSLATTSDHDFSYLSFAYDATDLELEGSYDYVIVGGGTSGC 66

Query: 81  PLAATLSSKFRVLVLERGGVPHGNSNLMSQEGFLTTLMDDVHSRNSPVQAFTSEEGVPNA 140
           PLAATLS K++VLVLERG +P    N+++ +GF+  L  +   + +PV+ F SE+G+ N 
Sbjct: 67  PLAATLSEKYKVLVLERGSLPTAYPNVLTADGFVYNLQQEDDGK-TPVERFVSEDGIDNV 126

Query: 141 RGRILGGSSAINAGFYSRADQAFFKNSQLKWDLAIVNQSYEWVEKEIVFKPNLKNWQSAV 200
           RGR+LGG+S INAG Y+RA+ + +  S + WD+ +VNQ+YEWVE  IV+KPN ++WQS  
Sbjct: 127 RGRVLGGTSIINAGVYARANTSIYSASGVDWDMDLVNQTYEWVEDTIVYKPNSQSWQSVT 186

Query: 201 RDGMVEAGIIPYNGFSLDHVMGTKIGGSTFDELGRRHSAADLLSYATPFNIKVAIYATVE 260
           +   +EAG+ P +GFSLDH  GT+I GSTFD  G RH+A +LL+     N++V ++A+VE
Sbjct: 187 KTAFLEAGVHPNHGFSLDHEEGTRITGSTFDNKGTRHAADELLNKGNSNNLRVGVHASVE 246

Query: 261 RILLASTVKRKPKAIGVLYRDQIGTYHHAMLHARGEVFLSAGAIGSPQLLLLSGIGSRPY 320
           +I+ ++       A GV+YRD  GT H A + ++GEV +SAG IG+PQLLLLSG+G   Y
Sbjct: 247 KIIFSNAPGL--TATGVIYRDSNGTPHQAFVRSKGEVIVSAGTIGTPQLLLLSGVGPESY 306

Query: 321 LSSWGIPVAHHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITESGTYLEAASNVIP 380
           LSS  IPV    PYVG +L+DNPRN I+IL P P+E +++ V GI  S  + + + + +P
Sbjct: 307 LSSLNIPVVLSHPYVGQFLHDNPRNFINILPPNPIEPTIVTVLGI--SNDFYQCSFSSLP 366

Query: 381 FTSPPRSFFIRTPSPPVYLTVATLMEKITGPVASGSMRL-ASTDVRVNPVVRFNYFGNAV 440
           FT+PP  FF     P    T A    K+ GP++ GS+ L +S++VRV+P V+FNY+ N  
Sbjct: 367 FTTPPFGFFPSASYPLPNSTFAHFASKVAGPLSYGSLTLKSSSNVRVSPNVKFNYYSNLT 426

Query: 441 DLERCVNGTRKIGEILRSQAMEGFKFRDWFGRKEFRYVGPAYPEKEDEDGLVLREFCRQS 500
           DL  CV+G +KIGE+L + A++ +K  D  G + F  +G   P K+  D      FCR+S
Sbjct: 427 DLSHCVSGMKKIGELLSTDALKPYKVEDLPGVEGFNILGIPLP-KDQTDDAAFETFCRES 486

Query: 501 VSTIWHYHGGCVAGKVVDSNYRVIGMEALRVVDGSIFGVSPGTNPQATVMMLGRHVGLQI 560
           V++ WHYHGGC+ GKV+D ++RV G+ ALRVVDGS F  +P ++PQ   +MLGR+VG++I
Sbjct: 487 VASYWHYHGGCLVGKVLDGDFRVTGINALRVVDGSTFPYTPASHPQGFYLMLGRYVGIKI 546

Query: 561 INQRSA 566
           + +RSA
Sbjct: 547 LQERSA 546

BLAST of Chy1G021200 vs. ExPASy TrEMBL
Match: A0A1S3CHZ4 ((R)-mandelonitrile lyase-like OS=Cucumis melo OX=3656 GN=LOC103500635 PE=3 SV=1)

HSP 1 Score: 1118.6 bits (2892), Expect = 0.0e+00
Identity = 554/573 (96.68%), Postives = 564/573 (98.43%), Query Frame = 0

Query: 1   MPLFPSSKKHSQAFLSPLMASRIFFHSQILTFLLIFSASSALPNSSQQDPDYLKFVSNAT 60
           MPLF SSKKHSQAFLSPLMASRIFFHSQILTFLLIFSASSALPNSSQ+DPDYLKFVSNAT
Sbjct: 1   MPLFTSSKKHSQAFLSPLMASRIFFHSQILTFLLIFSASSALPNSSQKDPDYLKFVSNAT 60

Query: 61  EFPEVDYYDYIIIGGGTAGCPLAATLSSKFRVLVLERGGVPHGNSNLMSQEGFLTTLMDD 120
           EFP+VDYYDYII+GGGTAGCPLAATLS+KFRVLVLERGG PHGNSNLMSQEGFLTTLMDD
Sbjct: 61  EFPKVDYYDYIIVGGGTAGCPLAATLSNKFRVLVLERGGFPHGNSNLMSQEGFLTTLMDD 120

Query: 121 VHSRNSPVQAFTSEEGVPNARGRILGGSSAINAGFYSRADQAFFKNSQLKWDLAIVNQSY 180
           VHSR SPVQAFTSEEGVPNARGRILGGSSAINAGFYSRADQAFFKNSQLKWDLAIVNQSY
Sbjct: 121 VHSRYSPVQAFTSEEGVPNARGRILGGSSAINAGFYSRADQAFFKNSQLKWDLAIVNQSY 180

Query: 181 EWVEKEIVFKPNLKNWQSAVRDGMVEAGIIPYNGFSLDHVMGTKIGGSTFDELGRRHSAA 240
           EWVEKEIVFKPNLKNWQSAVRDGMVEAGI PYNGFSLDHV+GTKIGGSTFDELGRRHSAA
Sbjct: 181 EWVEKEIVFKPNLKNWQSAVRDGMVEAGITPYNGFSLDHVIGTKIGGSTFDELGRRHSAA 240

Query: 241 DLLSYATPFNIKVAIYATVERILLASTVKRKPKAIGVLYRDQIGTYHHAMLHARGEVFLS 300
           DLLSYATPFNIKVAIYATVERILL STVKRKPKAIGVLYRDQIG YHHAMLHARGEVFLS
Sbjct: 241 DLLSYATPFNIKVAIYATVERILLGSTVKRKPKAIGVLYRDQIGKYHHAMLHARGEVFLS 300

Query: 301 AGAIGSPQLLLLSGIGSRPYLSSWGIPVAHHLPYVGHYLYDNPRNGISILAPMPLEQSLI 360
           AGAIGSPQLLLLSGIGSRPYLSSWGIPVAHHLPYVGHYLYDNPR GISILAPMPLEQSLI
Sbjct: 301 AGAIGSPQLLLLSGIGSRPYLSSWGIPVAHHLPYVGHYLYDNPRTGISILAPMPLEQSLI 360

Query: 361 QVAGITESGTYLEAASNVIPFTSPPRSFFIRTPSPPVYLTVATLMEKITGPVASGSMRLA 420
           QVAGITESGTYLEAASNVIPFT+PPRSFFIRTPSPPVYLTVATLMEKITGPVASG+MRLA
Sbjct: 361 QVAGITESGTYLEAASNVIPFTTPPRSFFIRTPSPPVYLTVATLMEKITGPVASGAMRLA 420

Query: 421 STDVRVNPVVRFNYFGNAVDLERCVNGTRKIGEILRSQAMEGFKFRDWFGRKEFRYVGPA 480
           STDVRVNPVVRFNYFGNAVDLERCVNGTRKIG+ILRSQAMEGFKFRDWFGRKEFR+VGPA
Sbjct: 421 STDVRVNPVVRFNYFGNAVDLERCVNGTRKIGDILRSQAMEGFKFRDWFGRKEFRFVGPA 480

Query: 481 YPEKEDEDGLVLREFCRQSVSTIWHYHGGCVAGKVVDSNYRVIGMEALRVVDGSIFGVSP 540
           YPE  DEDGLVLREFCRQSVSTIWHYHGGCVAGKVVDSNYRV+GMEALRVVDGSIFGVSP
Sbjct: 481 YPEDGDEDGLVLREFCRQSVSTIWHYHGGCVAGKVVDSNYRVMGMEALRVVDGSIFGVSP 540

Query: 541 GTNPQATVMMLGRHVGLQIINQRSAVTTHRVVY 574
           GTNPQATVMMLGRHVGLQIINQRSAVTTHRVVY
Sbjct: 541 GTNPQATVMMLGRHVGLQIINQRSAVTTHRVVY 573

BLAST of Chy1G021200 vs. ExPASy TrEMBL
Match: A0A5D3C0K1 ((R)-mandelonitrile lyase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G001740 PE=3 SV=1)

HSP 1 Score: 1115.1 bits (2883), Expect = 0.0e+00
Identity = 552/573 (96.34%), Postives = 562/573 (98.08%), Query Frame = 0

Query: 1   MPLFPSSKKHSQAFLSPLMASRIFFHSQILTFLLIFSASSALPNSSQQDPDYLKFVSNAT 60
           MPLF SSKKHSQAFLSPLMASRIFFHSQILTFLLIFSASSALPNSSQ+DPDYLKFVSNAT
Sbjct: 1   MPLFTSSKKHSQAFLSPLMASRIFFHSQILTFLLIFSASSALPNSSQKDPDYLKFVSNAT 60

Query: 61  EFPEVDYYDYIIIGGGTAGCPLAATLSSKFRVLVLERGGVPHGNSNLMSQEGFLTTLMDD 120
           EFP+VDYYDYII+GGGTAGCPLAATLS+KFRVLVLERGG PHGNSNLMSQEGFLTTLMDD
Sbjct: 61  EFPKVDYYDYIIVGGGTAGCPLAATLSNKFRVLVLERGGFPHGNSNLMSQEGFLTTLMDD 120

Query: 121 VHSRNSPVQAFTSEEGVPNARGRILGGSSAINAGFYSRADQAFFKNSQLKWDLAIVNQSY 180
           VHSR SPVQAFTSEEGVPNARGRILGGSSAINAGFYSRADQAFFKNSQLKWDLAIVNQSY
Sbjct: 121 VHSRYSPVQAFTSEEGVPNARGRILGGSSAINAGFYSRADQAFFKNSQLKWDLAIVNQSY 180

Query: 181 EWVEKEIVFKPNLKNWQSAVRDGMVEAGIIPYNGFSLDHVMGTKIGGSTFDELGRRHSAA 240
           EWVEKEIVFKPNLKNWQSAVRDGMVEAGI PYNGFSLDHV+GTKIGGSTFDELGRRHSAA
Sbjct: 181 EWVEKEIVFKPNLKNWQSAVRDGMVEAGITPYNGFSLDHVIGTKIGGSTFDELGRRHSAA 240

Query: 241 DLLSYATPFNIKVAIYATVERILLASTVKRKPKAIGVLYRDQIGTYHHAMLHARGEVFLS 300
           DLLSYATPFNIKVAIYATVERILL STVKRKPKAIGVLYRDQIG YHHAMLHARGEVFLS
Sbjct: 241 DLLSYATPFNIKVAIYATVERILLGSTVKRKPKAIGVLYRDQIGKYHHAMLHARGEVFLS 300

Query: 301 AGAIGSPQLLLLSGIGSRPYLSSWGIPVAHHLPYVGHYLYDNPRNGISILAPMPLEQSLI 360
           AGAIGSPQLLLLSGIGSRPYLSSWGIPV HHLPYVGHYLYDNPR GISILAPMPLEQSLI
Sbjct: 301 AGAIGSPQLLLLSGIGSRPYLSSWGIPVVHHLPYVGHYLYDNPRTGISILAPMPLEQSLI 360

Query: 361 QVAGITESGTYLEAASNVIPFTSPPRSFFIRTPSPPVYLTVATLMEKITGPVASGSMRLA 420
           QVAGITESGTYLEAASNVIPFT+PPRSFFIRTPSPPVYLTVATLMEKITGPVASG+MRLA
Sbjct: 361 QVAGITESGTYLEAASNVIPFTTPPRSFFIRTPSPPVYLTVATLMEKITGPVASGAMRLA 420

Query: 421 STDVRVNPVVRFNYFGNAVDLERCVNGTRKIGEILRSQAMEGFKFRDWFGRKEFRYVGPA 480
           STDVRVNPVVRFNYFGNAVDLERCVNGTRKIG+ILRSQAMEGFKFRDWFGRKEFR+VGPA
Sbjct: 421 STDVRVNPVVRFNYFGNAVDLERCVNGTRKIGDILRSQAMEGFKFRDWFGRKEFRFVGPA 480

Query: 481 YPEKEDEDGLVLREFCRQSVSTIWHYHGGCVAGKVVDSNYRVIGMEALRVVDGSIFGVSP 540
           YPE  DEDGLVLREFCRQSVSTIWHYHGGCVAGKVVDSNYRV+GMEALRVVDGSIFGVSP
Sbjct: 481 YPEDGDEDGLVLREFCRQSVSTIWHYHGGCVAGKVVDSNYRVMGMEALRVVDGSIFGVSP 540

Query: 541 GTNPQATVMMLGRHVGLQIINQRSAVTTHRVVY 574
           GTNPQATVMMLGRHVGLQIINQRSAVTTH VVY
Sbjct: 541 GTNPQATVMMLGRHVGLQIINQRSAVTTHHVVY 573

BLAST of Chy1G021200 vs. ExPASy TrEMBL
Match: A0A0A0KCB1 (GMC_OxRdtase_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G447930 PE=3 SV=1)

HSP 1 Score: 1107.0 bits (2862), Expect = 0.0e+00
Identity = 549/555 (98.92%), Postives = 552/555 (99.46%), Query Frame = 0

Query: 19  MASRIFFHSQILTFLLIFSASSALPNSSQQDPDYLKFVSNATEFPEVDYYDYIIIGGGTA 78
           M SRI FHSQILTFLLIFSASSALPNSSQQDPDYLKFVSNATEFPEVDYYDYII+GGGTA
Sbjct: 1   MESRISFHSQILTFLLIFSASSALPNSSQQDPDYLKFVSNATEFPEVDYYDYIIVGGGTA 60

Query: 79  GCPLAATLSSKFRVLVLERGGVPHGNSNLMSQEGFLTTLMDDVHSRNSPVQAFTSEEGVP 138
           GCPLAATLSSKFRVLVLERGGVPHGNSNLMSQEGFLTTLMDDVHSRNSPVQAFTSEEGVP
Sbjct: 61  GCPLAATLSSKFRVLVLERGGVPHGNSNLMSQEGFLTTLMDDVHSRNSPVQAFTSEEGVP 120

Query: 139 NARGRILGGSSAINAGFYSRADQAFFKNSQLKWDLAIVNQSYEWVEKEIVFKPNLKNWQS 198
           NARGRILGGSSAINAGFYSRADQAFFKNSQLKWDLAIVNQSYEWVEKEIVFKPNLKNWQS
Sbjct: 121 NARGRILGGSSAINAGFYSRADQAFFKNSQLKWDLAIVNQSYEWVEKEIVFKPNLKNWQS 180

Query: 199 AVRDGMVEAGIIPYNGFSLDHVMGTKIGGSTFDELGRRHSAADLLSYATPFNIKVAIYAT 258
           AVRDGMVEAGIIPYNGFSLDHVMGTKIGGSTFDELGRRHSAADLLS+ATPFNIKVAIYA 
Sbjct: 181 AVRDGMVEAGIIPYNGFSLDHVMGTKIGGSTFDELGRRHSAADLLSHATPFNIKVAIYAN 240

Query: 259 VERILLASTVKRKPKAIGVLYRDQIGTYHHAMLHARGEVFLSAGAIGSPQLLLLSGIGSR 318
           VERILLASTVKR+PKAIGVLYRDQIGTYHHAMLHARGEVFLSAGAIGSPQLLLLSGIGSR
Sbjct: 241 VERILLASTVKRRPKAIGVLYRDQIGTYHHAMLHARGEVFLSAGAIGSPQLLLLSGIGSR 300

Query: 319 PYLSSWGIPVAHHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITESGTYLEAASNV 378
           PYLSSWGIPVAHHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITESGTYLEAASNV
Sbjct: 301 PYLSSWGIPVAHHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITESGTYLEAASNV 360

Query: 379 IPFTSPPRSFFIRTPSPPVYLTVATLMEKITGPVASGSMRLASTDVRVNPVVRFNYFGNA 438
           IPFTSPPRSFFIRTPSPPVYLTVATLMEKITGPVASGSMRLASTDVRVNPVVRFNYFGNA
Sbjct: 361 IPFTSPPRSFFIRTPSPPVYLTVATLMEKITGPVASGSMRLASTDVRVNPVVRFNYFGNA 420

Query: 439 VDLERCVNGTRKIGEILRSQAMEGFKFRDWFGRKEFRYVGPAYPEKEDEDGLVLREFCRQ 498
           VDLERCVNGTRKIGEILRSQAMEGFKFRDWFGRKEFRYVGPAYPEKEDEDGLVLREFCRQ
Sbjct: 421 VDLERCVNGTRKIGEILRSQAMEGFKFRDWFGRKEFRYVGPAYPEKEDEDGLVLREFCRQ 480

Query: 499 SVSTIWHYHGGCVAGKVVDSNYRVIGMEALRVVDGSIFGVSPGTNPQATVMMLGRHVGLQ 558
           SVSTIWHYHGGCVAGKVVDSNYRVIGMEALRVVDGSIFGVSPGTNPQATVMMLGRHVGLQ
Sbjct: 481 SVSTIWHYHGGCVAGKVVDSNYRVIGMEALRVVDGSIFGVSPGTNPQATVMMLGRHVGLQ 540

Query: 559 IINQRSAVTTHRVVY 574
           IINQRSAVTTHRVVY
Sbjct: 541 IINQRSAVTTHRVVY 555

BLAST of Chy1G021200 vs. ExPASy TrEMBL
Match: A0A6J1JYK6 ((R)-mandelonitrile lyase-like OS=Cucurbita maxima OX=3661 GN=LOC111488606 PE=3 SV=1)

HSP 1 Score: 942.2 bits (2434), Expect = 1.0e-270
Identity = 470/575 (81.74%), Postives = 514/575 (89.39%), Query Frame = 0

Query: 1   MPLFPSSKKHSQAFLSPLMASRIFFHSQILTFLLIFSAS----SALPNSSQQDPDYLKFV 60
           MPLFP SKK +Q F+  +M  RIF +SQIL FLL+F AS    SA  +SSQ++P YLKFV
Sbjct: 1   MPLFP-SKKLTQTFVFSIMPPRIFSYSQILAFLLLFYASPASISADLSSSQREPSYLKFV 60

Query: 61  SNATEFPEVDYYDYIIIGGGTAGCPLAATLSSKFRVLVLERGGVPHGNSNLMSQEGFLTT 120
           S+A +FP+ DYYDYII+GGGTAGCPLAATLSSKFRVLVLERGGVPHGNSNLMSQEGFLTT
Sbjct: 61  SDAADFPKEDYYDYIIVGGGTAGCPLAATLSSKFRVLVLERGGVPHGNSNLMSQEGFLTT 120

Query: 121 LMDDVHSRNSPVQAFTSEEGVPNARGRILGGSSAINAGFYSRADQAFFKNSQLKWDLAIV 180
           LMDDVHSR+SPVQAFTSEEG+PNARGRILGGSSAINAGFYSRAD AFF+NS L WDLA+V
Sbjct: 121 LMDDVHSRDSPVQAFTSEEGIPNARGRILGGSSAINAGFYSRADPAFFQNSHLYWDLAMV 180

Query: 181 NQSYEWVEKEIVFKPNLKNWQSAVRDGMVEAGIIPYNGFSLDHVMGTKIGGSTFDELGRR 240
           NQSYEWVEKEIVF+PNLKNWQSAVRDGMVEAGI PY+GFS DH++GTKIGGSTFDELGRR
Sbjct: 181 NQSYEWVEKEIVFRPNLKNWQSAVRDGMVEAGIKPYHGFSFDHLIGTKIGGSTFDELGRR 240

Query: 241 HSAADLLSYATPFNIKVAIYATVERILLASTVKRKPKAIGVLYRDQIGTYHHAMLHARGE 300
           HSAADLL+YA P NIKVA++ATVERIL+AS    K  A GVLY DQ G YHHA+L ARGE
Sbjct: 241 HSAADLLAYAKPCNIKVAVHATVERILVAS----KATATGVLYHDQRGAYHHALLRARGE 300

Query: 301 VFLSAGAIGSPQLLLLSGIGSRPYLSSWGIPVAHHLPYVGHYLYDNPRNGISILAPMPLE 360
           V ++AGAIGSPQLLLLSGIGSRPYLSSWGIPV HHLPYVGHYLYDNPRNGISIL+PMPLE
Sbjct: 301 VLVAAGAIGSPQLLLLSGIGSRPYLSSWGIPVTHHLPYVGHYLYDNPRNGISILSPMPLE 360

Query: 361 QSLIQVAGITESGTYLEAASNVIPFTSPPRSFFIRTPSPPVYLTVATLMEKITGPVASGS 420
           QSLIQVAGITESG YLEA SNVIPFTSPP+SFFIR  S PVYLTVATLMEKI GPV+ GS
Sbjct: 361 QSLIQVAGITESGAYLEATSNVIPFTSPPKSFFIRAQSAPVYLTVATLMEKIIGPVSFGS 420

Query: 421 MRLASTDVRVNPVVRFNYFGNAVDLERCVNGTRKIGEILRSQAMEGFKFRDWFGRKEFRY 480
           MRLASTDVRVNPVV FNYFGNAVDLERCVNGTRKIGE+LRS++MEGFKF  W  ++EFR+
Sbjct: 421 MRLASTDVRVNPVVWFNYFGNAVDLERCVNGTRKIGEVLRSRSMEGFKFSGWLWKREFRF 480

Query: 481 VGPAYPEKEDEDGLVLREFCRQSVSTIWHYHGGCVAGKVVDSNYRVIGMEALRVVDGSIF 540
           VGP YPEKE++D   LREFC+++VSTIWHYHGGCVAGKVVD N+RVIGMEALRVVDGS F
Sbjct: 481 VGPVYPEKEEKDDGFLREFCKRTVSTIWHYHGGCVAGKVVDRNHRVIGMEALRVVDGSTF 540

Query: 541 GVSPGTNPQATVMMLGRHVGLQIINQRSAVTTHRV 572
           GVSPGTNPQAT+MMLGRHVGLQII +RS   THRV
Sbjct: 541 GVSPGTNPQATLMMLGRHVGLQIIKERSFTATHRV 570

BLAST of Chy1G021200 vs. ExPASy TrEMBL
Match: A0A6J1GR64 ((R)-mandelonitrile lyase-like OS=Cucurbita moschata OX=3662 GN=LOC111456379 PE=3 SV=1)

HSP 1 Score: 937.2 bits (2421), Expect = 3.3e-269
Identity = 468/575 (81.39%), Postives = 513/575 (89.22%), Query Frame = 0

Query: 1   MPLFPSSKKHSQAFLSPLMASRIFFHSQILTFLLIFSAS----SALPNSSQQDPDYLKFV 60
           M LFP SKK +Q F+   M  RIF +SQIL FLL+F AS    SA  +SSQ++P YLKFV
Sbjct: 1   MRLFP-SKKLTQTFVFSTMPPRIFSYSQILAFLLLFYASPASISADLSSSQREPSYLKFV 60

Query: 61  SNATEFPEVDYYDYIIIGGGTAGCPLAATLSSKFRVLVLERGGVPHGNSNLMSQEGFLTT 120
           S+A +FP+ DYYDYII+GGGTAGCPLAATLSSKFRVLVLERGGVPH NSNLMSQEGFLTT
Sbjct: 61  SDAADFPKEDYYDYIIVGGGTAGCPLAATLSSKFRVLVLERGGVPHANSNLMSQEGFLTT 120

Query: 121 LMDDVHSRNSPVQAFTSEEGVPNARGRILGGSSAINAGFYSRADQAFFKNSQLKWDLAIV 180
           LMDDVHSR+SPVQAFTSEEG+PNARGRILGGSSAINAGFYSRAD AFF+NS+L WDLA+V
Sbjct: 121 LMDDVHSRDSPVQAFTSEEGIPNARGRILGGSSAINAGFYSRADPAFFQNSRLHWDLAMV 180

Query: 181 NQSYEWVEKEIVFKPNLKNWQSAVRDGMVEAGIIPYNGFSLDHVMGTKIGGSTFDELGRR 240
           NQSYEWVEKEIVF+PNLKNWQSAVRDGMVEAGI PY+GFS DH++GTKIGGSTFDELGRR
Sbjct: 181 NQSYEWVEKEIVFRPNLKNWQSAVRDGMVEAGIKPYHGFSFDHLIGTKIGGSTFDELGRR 240

Query: 241 HSAADLLSYATPFNIKVAIYATVERILLASTVKRKPKAIGVLYRDQIGTYHHAMLHARGE 300
           HSAADLL+YA P NIKVA++ATVERIL+AS    K  A GVLY DQ G YHHA+L ARGE
Sbjct: 241 HSAADLLAYAKPCNIKVAVHATVERILVAS----KAAATGVLYHDQRGAYHHALLRARGE 300

Query: 301 VFLSAGAIGSPQLLLLSGIGSRPYLSSWGIPVAHHLPYVGHYLYDNPRNGISILAPMPLE 360
           V ++AGAIGSP LLLLSGIGSRPYLSSWGIPV HHLPYVGHYLYDNPRNGISIL+PMPLE
Sbjct: 301 VLVAAGAIGSPHLLLLSGIGSRPYLSSWGIPVTHHLPYVGHYLYDNPRNGISILSPMPLE 360

Query: 361 QSLIQVAGITESGTYLEAASNVIPFTSPPRSFFIRTPSPPVYLTVATLMEKITGPVASGS 420
           QSLIQVAGITESG YLEA SNVIPFTSPP+SFFIR  S PVYLTVATLMEKI GPV+ GS
Sbjct: 361 QSLIQVAGITESGAYLEATSNVIPFTSPPKSFFIRAQSAPVYLTVATLMEKIIGPVSFGS 420

Query: 421 MRLASTDVRVNPVVRFNYFGNAVDLERCVNGTRKIGEILRSQAMEGFKFRDWFGRKEFRY 480
           MRLASTDVRVNPVV FNYFGNAVDLERCVNGTRKIGE+LRS++MEGFKF  W  ++EFR+
Sbjct: 421 MRLASTDVRVNPVVWFNYFGNAVDLERCVNGTRKIGEVLRSRSMEGFKFSGWLWKREFRF 480

Query: 481 VGPAYPEKEDEDGLVLREFCRQSVSTIWHYHGGCVAGKVVDSNYRVIGMEALRVVDGSIF 540
           VGP YPEKE++D   LREFC+++VSTIWHYHGGCVAGKVVD N+RVIGMEALRVVDGS F
Sbjct: 481 VGPVYPEKEEKDDGFLREFCKRTVSTIWHYHGGCVAGKVVDRNHRVIGMEALRVVDGSTF 540

Query: 541 GVSPGTNPQATVMMLGRHVGLQIINQRSAVTTHRV 572
           GVSPGTNPQAT+MMLGRHVGLQII +RS+ TTHRV
Sbjct: 541 GVSPGTNPQATLMMLGRHVGLQIIKERSSTTTHRV 570

BLAST of Chy1G021200 vs. NCBI nr
Match: XP_008462229.1 (PREDICTED: (R)-mandelonitrile lyase-like [Cucumis melo])

HSP 1 Score: 1114 bits (2881), Expect = 0.0
Identity = 554/573 (96.68%), Postives = 564/573 (98.43%), Query Frame = 0

Query: 1   MPLFPSSKKHSQAFLSPLMASRIFFHSQILTFLLIFSASSALPNSSQQDPDYLKFVSNAT 60
           MPLF SSKKHSQAFLSPLMASRIFFHSQILTFLLIFSASSALPNSSQ+DPDYLKFVSNAT
Sbjct: 1   MPLFTSSKKHSQAFLSPLMASRIFFHSQILTFLLIFSASSALPNSSQKDPDYLKFVSNAT 60

Query: 61  EFPEVDYYDYIIIGGGTAGCPLAATLSSKFRVLVLERGGVPHGNSNLMSQEGFLTTLMDD 120
           EFP+VDYYDYII+GGGTAGCPLAATLS+KFRVLVLERGG PHGNSNLMSQEGFLTTLMDD
Sbjct: 61  EFPKVDYYDYIIVGGGTAGCPLAATLSNKFRVLVLERGGFPHGNSNLMSQEGFLTTLMDD 120

Query: 121 VHSRNSPVQAFTSEEGVPNARGRILGGSSAINAGFYSRADQAFFKNSQLKWDLAIVNQSY 180
           VHSR SPVQAFTSEEGVPNARGRILGGSSAINAGFYSRADQAFFKNSQLKWDLAIVNQSY
Sbjct: 121 VHSRYSPVQAFTSEEGVPNARGRILGGSSAINAGFYSRADQAFFKNSQLKWDLAIVNQSY 180

Query: 181 EWVEKEIVFKPNLKNWQSAVRDGMVEAGIIPYNGFSLDHVMGTKIGGSTFDELGRRHSAA 240
           EWVEKEIVFKPNLKNWQSAVRDGMVEAGI PYNGFSLDHV+GTKIGGSTFDELGRRHSAA
Sbjct: 181 EWVEKEIVFKPNLKNWQSAVRDGMVEAGITPYNGFSLDHVIGTKIGGSTFDELGRRHSAA 240

Query: 241 DLLSYATPFNIKVAIYATVERILLASTVKRKPKAIGVLYRDQIGTYHHAMLHARGEVFLS 300
           DLLSYATPFNIKVAIYATVERILL STVKRKPKAIGVLYRDQIG YHHAMLHARGEVFLS
Sbjct: 241 DLLSYATPFNIKVAIYATVERILLGSTVKRKPKAIGVLYRDQIGKYHHAMLHARGEVFLS 300

Query: 301 AGAIGSPQLLLLSGIGSRPYLSSWGIPVAHHLPYVGHYLYDNPRNGISILAPMPLEQSLI 360
           AGAIGSPQLLLLSGIGSRPYLSSWGIPVAHHLPYVGHYLYDNPR GISILAPMPLEQSLI
Sbjct: 301 AGAIGSPQLLLLSGIGSRPYLSSWGIPVAHHLPYVGHYLYDNPRTGISILAPMPLEQSLI 360

Query: 361 QVAGITESGTYLEAASNVIPFTSPPRSFFIRTPSPPVYLTVATLMEKITGPVASGSMRLA 420
           QVAGITESGTYLEAASNVIPFT+PPRSFFIRTPSPPVYLTVATLMEKITGPVASG+MRLA
Sbjct: 361 QVAGITESGTYLEAASNVIPFTTPPRSFFIRTPSPPVYLTVATLMEKITGPVASGAMRLA 420

Query: 421 STDVRVNPVVRFNYFGNAVDLERCVNGTRKIGEILRSQAMEGFKFRDWFGRKEFRYVGPA 480
           STDVRVNPVVRFNYFGNAVDLERCVNGTRKIG+ILRSQAMEGFKFRDWFGRKEFR+VGPA
Sbjct: 421 STDVRVNPVVRFNYFGNAVDLERCVNGTRKIGDILRSQAMEGFKFRDWFGRKEFRFVGPA 480

Query: 481 YPEKEDEDGLVLREFCRQSVSTIWHYHGGCVAGKVVDSNYRVIGMEALRVVDGSIFGVSP 540
           YPE  DEDGLVLREFCRQSVSTIWHYHGGCVAGKVVDSNYRV+GMEALRVVDGSIFGVSP
Sbjct: 481 YPEDGDEDGLVLREFCRQSVSTIWHYHGGCVAGKVVDSNYRVMGMEALRVVDGSIFGVSP 540

Query: 541 GTNPQATVMMLGRHVGLQIINQRSAVTTHRVVY 573
           GTNPQATVMMLGRHVGLQIINQRSAVTTHRVVY
Sbjct: 541 GTNPQATVMMLGRHVGLQIINQRSAVTTHRVVY 573

BLAST of Chy1G021200 vs. NCBI nr
Match: KAA0059350.1 ((R)-mandelonitrile lyase-like [Cucumis melo var. makuwa] >TYK03976.1 (R)-mandelonitrile lyase-like [Cucumis melo var. makuwa])

HSP 1 Score: 1110 bits (2872), Expect = 0.0
Identity = 552/573 (96.34%), Postives = 562/573 (98.08%), Query Frame = 0

Query: 1   MPLFPSSKKHSQAFLSPLMASRIFFHSQILTFLLIFSASSALPNSSQQDPDYLKFVSNAT 60
           MPLF SSKKHSQAFLSPLMASRIFFHSQILTFLLIFSASSALPNSSQ+DPDYLKFVSNAT
Sbjct: 1   MPLFTSSKKHSQAFLSPLMASRIFFHSQILTFLLIFSASSALPNSSQKDPDYLKFVSNAT 60

Query: 61  EFPEVDYYDYIIIGGGTAGCPLAATLSSKFRVLVLERGGVPHGNSNLMSQEGFLTTLMDD 120
           EFP+VDYYDYII+GGGTAGCPLAATLS+KFRVLVLERGG PHGNSNLMSQEGFLTTLMDD
Sbjct: 61  EFPKVDYYDYIIVGGGTAGCPLAATLSNKFRVLVLERGGFPHGNSNLMSQEGFLTTLMDD 120

Query: 121 VHSRNSPVQAFTSEEGVPNARGRILGGSSAINAGFYSRADQAFFKNSQLKWDLAIVNQSY 180
           VHSR SPVQAFTSEEGVPNARGRILGGSSAINAGFYSRADQAFFKNSQLKWDLAIVNQSY
Sbjct: 121 VHSRYSPVQAFTSEEGVPNARGRILGGSSAINAGFYSRADQAFFKNSQLKWDLAIVNQSY 180

Query: 181 EWVEKEIVFKPNLKNWQSAVRDGMVEAGIIPYNGFSLDHVMGTKIGGSTFDELGRRHSAA 240
           EWVEKEIVFKPNLKNWQSAVRDGMVEAGI PYNGFSLDHV+GTKIGGSTFDELGRRHSAA
Sbjct: 181 EWVEKEIVFKPNLKNWQSAVRDGMVEAGITPYNGFSLDHVIGTKIGGSTFDELGRRHSAA 240

Query: 241 DLLSYATPFNIKVAIYATVERILLASTVKRKPKAIGVLYRDQIGTYHHAMLHARGEVFLS 300
           DLLSYATPFNIKVAIYATVERILL STVKRKPKAIGVLYRDQIG YHHAMLHARGEVFLS
Sbjct: 241 DLLSYATPFNIKVAIYATVERILLGSTVKRKPKAIGVLYRDQIGKYHHAMLHARGEVFLS 300

Query: 301 AGAIGSPQLLLLSGIGSRPYLSSWGIPVAHHLPYVGHYLYDNPRNGISILAPMPLEQSLI 360
           AGAIGSPQLLLLSGIGSRPYLSSWGIPV HHLPYVGHYLYDNPR GISILAPMPLEQSLI
Sbjct: 301 AGAIGSPQLLLLSGIGSRPYLSSWGIPVVHHLPYVGHYLYDNPRTGISILAPMPLEQSLI 360

Query: 361 QVAGITESGTYLEAASNVIPFTSPPRSFFIRTPSPPVYLTVATLMEKITGPVASGSMRLA 420
           QVAGITESGTYLEAASNVIPFT+PPRSFFIRTPSPPVYLTVATLMEKITGPVASG+MRLA
Sbjct: 361 QVAGITESGTYLEAASNVIPFTTPPRSFFIRTPSPPVYLTVATLMEKITGPVASGAMRLA 420

Query: 421 STDVRVNPVVRFNYFGNAVDLERCVNGTRKIGEILRSQAMEGFKFRDWFGRKEFRYVGPA 480
           STDVRVNPVVRFNYFGNAVDLERCVNGTRKIG+ILRSQAMEGFKFRDWFGRKEFR+VGPA
Sbjct: 421 STDVRVNPVVRFNYFGNAVDLERCVNGTRKIGDILRSQAMEGFKFRDWFGRKEFRFVGPA 480

Query: 481 YPEKEDEDGLVLREFCRQSVSTIWHYHGGCVAGKVVDSNYRVIGMEALRVVDGSIFGVSP 540
           YPE  DEDGLVLREFCRQSVSTIWHYHGGCVAGKVVDSNYRV+GMEALRVVDGSIFGVSP
Sbjct: 481 YPEDGDEDGLVLREFCRQSVSTIWHYHGGCVAGKVVDSNYRVMGMEALRVVDGSIFGVSP 540

Query: 541 GTNPQATVMMLGRHVGLQIINQRSAVTTHRVVY 573
           GTNPQATVMMLGRHVGLQIINQRSAVTTH VVY
Sbjct: 541 GTNPQATVMMLGRHVGLQIINQRSAVTTHHVVY 573

BLAST of Chy1G021200 vs. NCBI nr
Match: KGN45431.1 (hypothetical protein Csa_016192 [Cucumis sativus])

HSP 1 Score: 1102 bits (2851), Expect = 0.0
Identity = 549/555 (98.92%), Postives = 552/555 (99.46%), Query Frame = 0

Query: 19  MASRIFFHSQILTFLLIFSASSALPNSSQQDPDYLKFVSNATEFPEVDYYDYIIIGGGTA 78
           M SRI FHSQILTFLLIFSASSALPNSSQQDPDYLKFVSNATEFPEVDYYDYII+GGGTA
Sbjct: 1   MESRISFHSQILTFLLIFSASSALPNSSQQDPDYLKFVSNATEFPEVDYYDYIIVGGGTA 60

Query: 79  GCPLAATLSSKFRVLVLERGGVPHGNSNLMSQEGFLTTLMDDVHSRNSPVQAFTSEEGVP 138
           GCPLAATLSSKFRVLVLERGGVPHGNSNLMSQEGFLTTLMDDVHSRNSPVQAFTSEEGVP
Sbjct: 61  GCPLAATLSSKFRVLVLERGGVPHGNSNLMSQEGFLTTLMDDVHSRNSPVQAFTSEEGVP 120

Query: 139 NARGRILGGSSAINAGFYSRADQAFFKNSQLKWDLAIVNQSYEWVEKEIVFKPNLKNWQS 198
           NARGRILGGSSAINAGFYSRADQAFFKNSQLKWDLAIVNQSYEWVEKEIVFKPNLKNWQS
Sbjct: 121 NARGRILGGSSAINAGFYSRADQAFFKNSQLKWDLAIVNQSYEWVEKEIVFKPNLKNWQS 180

Query: 199 AVRDGMVEAGIIPYNGFSLDHVMGTKIGGSTFDELGRRHSAADLLSYATPFNIKVAIYAT 258
           AVRDGMVEAGIIPYNGFSLDHVMGTKIGGSTFDELGRRHSAADLLS+ATPFNIKVAIYA 
Sbjct: 181 AVRDGMVEAGIIPYNGFSLDHVMGTKIGGSTFDELGRRHSAADLLSHATPFNIKVAIYAN 240

Query: 259 VERILLASTVKRKPKAIGVLYRDQIGTYHHAMLHARGEVFLSAGAIGSPQLLLLSGIGSR 318
           VERILLASTVKR+PKAIGVLYRDQIGTYHHAMLHARGEVFLSAGAIGSPQLLLLSGIGSR
Sbjct: 241 VERILLASTVKRRPKAIGVLYRDQIGTYHHAMLHARGEVFLSAGAIGSPQLLLLSGIGSR 300

Query: 319 PYLSSWGIPVAHHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITESGTYLEAASNV 378
           PYLSSWGIPVAHHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITESGTYLEAASNV
Sbjct: 301 PYLSSWGIPVAHHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITESGTYLEAASNV 360

Query: 379 IPFTSPPRSFFIRTPSPPVYLTVATLMEKITGPVASGSMRLASTDVRVNPVVRFNYFGNA 438
           IPFTSPPRSFFIRTPSPPVYLTVATLMEKITGPVASGSMRLASTDVRVNPVVRFNYFGNA
Sbjct: 361 IPFTSPPRSFFIRTPSPPVYLTVATLMEKITGPVASGSMRLASTDVRVNPVVRFNYFGNA 420

Query: 439 VDLERCVNGTRKIGEILRSQAMEGFKFRDWFGRKEFRYVGPAYPEKEDEDGLVLREFCRQ 498
           VDLERCVNGTRKIGEILRSQAMEGFKFRDWFGRKEFRYVGPAYPEKEDEDGLVLREFCRQ
Sbjct: 421 VDLERCVNGTRKIGEILRSQAMEGFKFRDWFGRKEFRYVGPAYPEKEDEDGLVLREFCRQ 480

Query: 499 SVSTIWHYHGGCVAGKVVDSNYRVIGMEALRVVDGSIFGVSPGTNPQATVMMLGRHVGLQ 558
           SVSTIWHYHGGCVAGKVVDSNYRVIGMEALRVVDGSIFGVSPGTNPQATVMMLGRHVGLQ
Sbjct: 481 SVSTIWHYHGGCVAGKVVDSNYRVIGMEALRVVDGSIFGVSPGTNPQATVMMLGRHVGLQ 540

Query: 559 IINQRSAVTTHRVVY 573
           IINQRSAVTTHRVVY
Sbjct: 541 IINQRSAVTTHRVVY 555

BLAST of Chy1G021200 vs. NCBI nr
Match: XP_011659801.1 ((R)-mandelonitrile lyase-like, partial [Cucumis sativus])

HSP 1 Score: 1055 bits (2728), Expect = 0.0
Identity = 521/525 (99.24%), Postives = 524/525 (99.81%), Query Frame = 0

Query: 49  DPDYLKFVSNATEFPEVDYYDYIIIGGGTAGCPLAATLSSKFRVLVLERGGVPHGNSNLM 108
           DPDYLKFVSNATEFPEVDYYDYII+GGGTAGCPLAATLSSKFRVLVLERGGVPHGNSNLM
Sbjct: 8   DPDYLKFVSNATEFPEVDYYDYIIVGGGTAGCPLAATLSSKFRVLVLERGGVPHGNSNLM 67

Query: 109 SQEGFLTTLMDDVHSRNSPVQAFTSEEGVPNARGRILGGSSAINAGFYSRADQAFFKNSQ 168
           SQEGFLTTLMDDVHSRNSPVQAFTSEEGVPNARGRILGGSSAINAGFYSRADQAFFKNSQ
Sbjct: 68  SQEGFLTTLMDDVHSRNSPVQAFTSEEGVPNARGRILGGSSAINAGFYSRADQAFFKNSQ 127

Query: 169 LKWDLAIVNQSYEWVEKEIVFKPNLKNWQSAVRDGMVEAGIIPYNGFSLDHVMGTKIGGS 228
           LKWDLAIVNQSYEWVEKEIVFKPNLKNWQSAVRDGMVEAGIIPYNGFSLDHVMGTKIGGS
Sbjct: 128 LKWDLAIVNQSYEWVEKEIVFKPNLKNWQSAVRDGMVEAGIIPYNGFSLDHVMGTKIGGS 187

Query: 229 TFDELGRRHSAADLLSYATPFNIKVAIYATVERILLASTVKRKPKAIGVLYRDQIGTYHH 288
           TFDELGRRHSAADLLS+ATPFNIKVAIYA VERILLASTVKR+PKAIGVLYRDQIGTYHH
Sbjct: 188 TFDELGRRHSAADLLSHATPFNIKVAIYANVERILLASTVKRRPKAIGVLYRDQIGTYHH 247

Query: 289 AMLHARGEVFLSAGAIGSPQLLLLSGIGSRPYLSSWGIPVAHHLPYVGHYLYDNPRNGIS 348
           AMLHARGEVFLSAGAIGSPQLLLLSGIGSRPYLSSWGIPVAHHLPYVGHYLYDNPRNGIS
Sbjct: 248 AMLHARGEVFLSAGAIGSPQLLLLSGIGSRPYLSSWGIPVAHHLPYVGHYLYDNPRNGIS 307

Query: 349 ILAPMPLEQSLIQVAGITESGTYLEAASNVIPFTSPPRSFFIRTPSPPVYLTVATLMEKI 408
           ILAPMPLEQSLIQVAGITESGTYLEAASNVIPFTSPPRSFFIRTPSPPVYLTVATLMEKI
Sbjct: 308 ILAPMPLEQSLIQVAGITESGTYLEAASNVIPFTSPPRSFFIRTPSPPVYLTVATLMEKI 367

Query: 409 TGPVASGSMRLASTDVRVNPVVRFNYFGNAVDLERCVNGTRKIGEILRSQAMEGFKFRDW 468
           TGPVASGSMRLASTDVRVNPVVRFNYFGNAVDLERCVNGTRKIGEILRSQAMEGFKFRDW
Sbjct: 368 TGPVASGSMRLASTDVRVNPVVRFNYFGNAVDLERCVNGTRKIGEILRSQAMEGFKFRDW 427

Query: 469 FGRKEFRYVGPAYPEKEDEDGLVLREFCRQSVSTIWHYHGGCVAGKVVDSNYRVIGMEAL 528
           FGRKEFRYVGPAYPEKEDEDGLVLREFCRQSVSTIWHYHGGCVAGKVVDSNYRVIGMEAL
Sbjct: 428 FGRKEFRYVGPAYPEKEDEDGLVLREFCRQSVSTIWHYHGGCVAGKVVDSNYRVIGMEAL 487

Query: 529 RVVDGSIFGVSPGTNPQATVMMLGRHVGLQIINQRSAVTTHRVVY 573
           RVVDGSIFGVSPGTNPQATVMMLGRHVGLQIINQRSAVTTHRVVY
Sbjct: 488 RVVDGSIFGVSPGTNPQATVMMLGRHVGLQIINQRSAVTTHRVVY 532

BLAST of Chy1G021200 vs. NCBI nr
Match: XP_038899448.1 ((R)-mandelonitrile lyase-like [Benincasa hispida])

HSP 1 Score: 1042 bits (2694), Expect = 0.0
Identity = 517/572 (90.38%), Postives = 540/572 (94.41%), Query Frame = 0

Query: 1   MPLFPSSKKHSQAFLSPLMASRIFFHSQILTFLLIFSASSALPNSSQQDPDYLKFVSNAT 60
           MPLFPSSKKH+QAF SPLMA R FFHSQ L FLLIF AS A PNSSQ++PDYLKFV+NAT
Sbjct: 1   MPLFPSSKKHTQAFFSPLMAPRFFFHSQFLAFLLIFYASFAHPNSSQREPDYLKFVANAT 60

Query: 61  EFPEVDYYDYIIIGGGTAGCPLAATLSSKFRVLVLERGGVPHGNSNLMSQEGFLTTLMDD 120
           EFPE DYYDYII+GGGTAGCPLAATLSSKFRVLVLERGGVPHGNSNLMSQEGFLTTLMDD
Sbjct: 61  EFPEEDYYDYIIVGGGTAGCPLAATLSSKFRVLVLERGGVPHGNSNLMSQEGFLTTLMDD 120

Query: 121 VHSRNSPVQAFTSEEGVPNARGRILGGSSAINAGFYSRADQAFFKNSQLKWDLAIVNQSY 180
           VHSR+SPVQAFTSEEGVPNARGRILGGSSAINAGFYSRADQAFFKNS+L WDLA+VNQSY
Sbjct: 121 VHSRDSPVQAFTSEEGVPNARGRILGGSSAINAGFYSRADQAFFKNSRLNWDLAMVNQSY 180

Query: 181 EWVEKEIVFKPNLKNWQSAVRDGMVEAGIIPYNGFSLDHVMGTKIGGSTFDELGRRHSAA 240
           EWVEKEIVF+PNLKNWQSAVRDGMVE GI PYNGFSLDH++GTKIGGSTFDELGRRHSAA
Sbjct: 181 EWVEKEIVFRPNLKNWQSAVRDGMVEVGINPYNGFSLDHLIGTKIGGSTFDELGRRHSAA 240

Query: 241 DLLSYATPFNIKVAIYATVERILLASTVK-RKPKAIGVLYRDQIGTYHHAMLHARGEVFL 300
           DLLSYATP NIKVA+YATVERILLAS    R+  AIGVLYRDQ G YHHAML ARGEV +
Sbjct: 241 DLLSYATPINIKVAVYATVERILLASGGPLRRTTAIGVLYRDQKGAYHHAMLRARGEVLV 300

Query: 301 SAGAIGSPQLLLLSGIGSRPYLSSWGIPVAHHLPYVGHYLYDNPRNGISILAPMPLEQSL 360
           SAGAIGSPQLLLLSGIGSRPYLSSWGIPV HHLPYVGHYLYDNPRNGISILAPMPLEQSL
Sbjct: 301 SAGAIGSPQLLLLSGIGSRPYLSSWGIPVTHHLPYVGHYLYDNPRNGISILAPMPLEQSL 360

Query: 361 IQVAGITESGTYLEAASNVIPFTSPPRSFFIRTPSPPVYLTVATLMEKITGPVASGSMRL 420
           IQVAGITESG YLEAASNVIPFTSPPRSFFIRTPS PVYLTVATLMEKITGPV+SGSMRL
Sbjct: 361 IQVAGITESGAYLEAASNVIPFTSPPRSFFIRTPSAPVYLTVATLMEKITGPVSSGSMRL 420

Query: 421 ASTDVRVNPVVRFNYFGNAVDLERCVNGTRKIGEILRSQAMEGFKFRDWFGRKEFRYVGP 480
           ASTDVRVNPVVRFNYFGNAVDLERCVNGTRKIGEILRS++MEGFKFRDW GR EFR+VGP
Sbjct: 421 ASTDVRVNPVVRFNYFGNAVDLERCVNGTRKIGEILRSRSMEGFKFRDWLGRSEFRFVGP 480

Query: 481 AYPEKEDEDGLVLREFCRQSVSTIWHYHGGCVAGKVVDSNYRVIGMEALRVVDGSIFGVS 540
            YPE EDEDG +LREFCR+SVSTIWHYHGGCVAGKVVDSN RVIGMEALRVVDGSIFGVS
Sbjct: 481 VYPENEDEDGSMLREFCRRSVSTIWHYHGGCVAGKVVDSNSRVIGMEALRVVDGSIFGVS 540

Query: 541 PGTNPQATVMMLGRHVGLQIINQRSAVTTHRV 571
           PGTNPQAT+MMLGRHVGLQIINQRSA+TTHR+
Sbjct: 541 PGTNPQATLMMLGRHVGLQIINQRSAITTHRI 572

BLAST of Chy1G021200 vs. TAIR 10
Match: AT1G73050.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 724.9 bits (1870), Expect = 4.9e-209
Identity = 349/538 (64.87%), Postives = 435/538 (80.86%), Query Frame = 0

Query: 29  ILTFLLIFSASSALPNSSQQDPDYLKFVSNATEFPEVDYYDYIIIGGGTAGCPLAATLSS 88
           +L  L +   S+A P  + + P +++F+SNAT+F   DYYDYII+GGGTAGCPLAATLS 
Sbjct: 16  VLLLLGVVHRSNARPRVN-RPPGFMRFISNATDFASEDYYDYIIVGGGTAGCPLAATLSQ 75

Query: 89  KFRVLVLERGGVPHGNSNLMSQEGFLTTLMDDVHSRNSPVQAFTSEEGVPNARGRILGGS 148
            FRVL+LERGGVP+   N+MS +GFLTTL  DV++ +SP Q+F SEEGVPNARGR+LGGS
Sbjct: 76  SFRVLLLERGGVPYNRPNVMSHDGFLTTL-TDVNNFDSPAQSFISEEGVPNARGRVLGGS 135

Query: 149 SAINAGFYSRADQAFFKNSQLKWDLAIVNQSYEWVEKEIVFKPNLKNWQSAVRDGMVEAG 208
           SAINAGFYSRAD+ FF+NS L WDL+ VNQSYEWVE+ IVF+P L+ WQ+A+RD ++E G
Sbjct: 136 SAINAGFYSRADKQFFENSGLVWDLSSVNQSYEWVERAIVFRPQLRTWQTAIRDALLEVG 195

Query: 209 IIPYNGFSLDHVMGTKIGGSTFDELGRRHSAADLLSYATPFNIKVAIYATVERILLASTV 268
           + P+NGF+L+H +GTKIGGSTFD  GRRHS+ADLL YA   NI+VA+YATVER+LLAS+ 
Sbjct: 196 VHPFNGFTLEHKVGTKIGGSTFDRTGRRHSSADLLRYARSSNIRVAVYATVERVLLASSP 255

Query: 269 K---RKPKAIGVLYRDQIGTYHHAMLHARGEVFLSAGAIGSPQLLLLSGIGSRPYLSSWG 328
                   AIGV+YRDQ+G +HHA++  RGEV LSAGA+GSPQLL LSGIG R YLS+WG
Sbjct: 256 SVSGSNVSAIGVVYRDQLGRFHHALIRDRGEVILSAGALGSPQLLFLSGIGPRSYLSTWG 315

Query: 329 IPVAHHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITESGTYLEAASNVIPFTSPP 388
           IPVA   P+VG ++YDNPRNGISI+ P+P+E SLIQV G+TE G +LEAASNVIPF SP 
Sbjct: 316 IPVALDQPHVGDFVYDNPRNGISIVPPVPMENSLIQVVGVTEDGAFLEAASNVIPFASPL 375

Query: 389 RSFFIRTPSPPVYLTVATLMEKITGPVASGSMRLASTDVRVNPVVRFNYFGNAVDLERCV 448
            S FIR P+ P+Y+ V T+MEKI GPV+ G +RLASTDVR+NPVVRFNYF +  DLERCV
Sbjct: 376 HSVFIRAPASPLYVPVTTIMEKILGPVSIGLLRLASTDVRINPVVRFNYFSDPQDLERCV 435

Query: 449 NGTRKIGEILRSQAMEGFKFRDWFGRKEFRYVGPAYPEKEDEDGLVLREFCRQSVSTIWH 508
           NGTRKIGEILRS+AM+ F  R+WFG + FR+VG   P  +  D LV+ +FCR++VSTIWH
Sbjct: 436 NGTRKIGEILRSRAMQDFMIREWFGNRRFRFVGAPLPVDQSND-LVMADFCRRTVSTIWH 495

Query: 509 YHGGCVAGKVVDSNYRVIGMEALRVVDGSIFGVSPGTNPQATVMMLGRHVGLQIINQR 564
           YHGG V GKVVDS+ +VIG+ +LR+VDGS F +SPGTNPQAT+MMLGR++GL+++ +R
Sbjct: 496 YHGGAVVGKVVDSDLKVIGVNSLRLVDGSTFNISPGTNPQATLMMLGRYMGLKMLRER 550

BLAST of Chy1G021200 vs. TAIR 10
Match: AT1G12570.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 468.8 bits (1205), Expect = 6.4e-132
Identity = 254/561 (45.28%), Postives = 347/561 (61.85%), Query Frame = 0

Query: 45  SSQQDPDYLKFVSNATEFPEVDYYDYIIIGGGTAGCPLAATLSSKFRVLVLERGGVPHGN 104
           SS + P+Y  F+ +AT  P   YYDYIIIGGGTAGCPLAATLS    VL+LERG  P+ N
Sbjct: 24  SSDKAPNY-SFMRDATGSPTTSYYDYIIIGGGTAGCPLAATLSQNASVLLLERGDSPYNN 83

Query: 105 SNLMSQEGFLTTLMDDVHSRNSPVQAFTSEEGVPNARGRILGGSSAINAGFYSRADQAFF 164
            N+     F   L D   S +SP Q F SE+GV NAR R+LGG SA+NAGFY+RA   + 
Sbjct: 84  PNITRLSAFGAALSD--LSESSPSQRFVSEDGVINARARVLGGGSALNAGFYTRAGTKYV 143

Query: 165 KNSQLKWDLAIVNQSYEWVEKEIVFKPNLKNWQSAVRDGMVEAGIIPYNGFSLDHVMGTK 224
           +N  + WD A+ N+SY+WVE ++ F+P +  WQ+AVRDG++EAGI+P NGF+ DH+ GTK
Sbjct: 144 RN--MGWDGALANESYQWVEAKVAFQPPMGRWQTAVRDGLLEAGIVPNNGFTYDHINGTK 203

Query: 225 IGGSTFDELGRRHSAADLLSYATPFNIKVAIYATVERILLASTVKRKPKAIGVLYRDQIG 284
            GG+ FD  G RH+AADLL YA P  I V ++ATV RIL  +    KP A GV+YRD+ G
Sbjct: 204 FGGTIFDRNGNRHTAADLLEYADPKGITVLLHATVHRILFRTRGTTKPIANGVVYRDRTG 263

Query: 285 TYHHAMLH--ARGEVFLSAGAIGSPQLLLLSGIGSRPYLSSWGIPVAHHLPYVGHYLYDN 344
             H A L   A  E+ LSAG +GSPQLL+LSG+G    L +  I V    P+VG  +YDN
Sbjct: 264 QAHRAYLKEGALSEIILSAGTLGSPQLLMLSGVGPSAQLQAQNITVVMDQPHVGQGMYDN 323

Query: 345 PRNGISILAPMPLEQSLIQVAGITESGTYLEAA---------------SNVIPFTS--PP 404
           P N + + +P+P+E SLI+V GIT  GTY+EAA               S+   + +   P
Sbjct: 324 PMNAVFVPSPVPVEVSLIEVVGITGEGTYVEAAGGENFGGGGGGSSGSSSTRDYYAMFSP 383

Query: 405 RSFFIRTPSPPVYLTV-----ATLMEKITGPVASGSMRLASTDVRVNPVVRFNYFGNAVD 464
           R+  + + S     +        L+EK+ GP+++G + L + + + NP+V FNYF +  D
Sbjct: 384 RATLLESNSMTKLSSAQPFQGGFLLEKVMGPLSTGHLELKTRNPKDNPIVTFNYFQHPDD 443

Query: 465 LERCVNGTRKIGEILRSQAMEGFKFRDWFGRKEFRYV------------------GPAYP 524
           L+RCV G + I  +++S+A   +K+ D      F Y+                  G + P
Sbjct: 444 LKRCVRGIQTIERVVQSKAFSRYKYAD----VSFEYLLNLTASTPVNLRPPRSGPGASLP 503

Query: 525 EKEDEDGLVLREFCRQSVSTIWHYHGGCVAGKVVDSNYRVIGMEALRVVDGSIFGVSPGT 564
              +       EFC+ +V+TIWHYHGGCV G+VVD +Y+VIG++ LRV+D S  G  PGT
Sbjct: 504 PSAE-------EFCQHTVTTIWHYHGGCVVGRVVDGDYKVIGIDRLRVIDMSTVGYCPGT 563

BLAST of Chy1G021200 vs. TAIR 10
Match: AT5G51950.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 449.1 bits (1154), Expect = 5.2e-126
Identity = 259/580 (44.66%), Postives = 342/580 (58.97%), Query Frame = 0

Query: 23  IFFHSQILTFLLIFSASSALPNSSQQDPDYLKFVSNATEFPEVDYYDYIIIGGGTAGCPL 82
           ++   +I     IF AS      S     Y  F+ +AT  P    +DYIIIGGGT+GC L
Sbjct: 6   LWLFRKIFIITYIFHAS-----CSPDKAGYYSFMKDATLAPMFARFDYIIIGGGTSGCAL 65

Query: 83  AATLSSKFRVLVLERGGVPHGNSNLMSQEGFLTTLMDDVHSRNSPVQAFTSEEGVPNARG 142
           AATLS    VLVLERGG P+ N      E F TTL +   S  S  Q F SE+GV N R 
Sbjct: 66  AATLSQNASVLVLERGGAPYDNPTATDIENFATTLSNT--SPKSWSQLFISEDGVYNTRA 125

Query: 143 RILGGSSAINAGFYSRADQAFFKNSQLKWDLAIVNQSYEWVEKEIVFKPNLKNWQSAVRD 202
           R+LGG S +NAGFY+RA   + K ++  W    V  +YEWVEK++ F+P +  WQ+A +D
Sbjct: 126 RVLGGGSVLNAGFYTRAGDEYVKETE--WKTDEVEAAYEWVEKKVAFQPPVLGWQTAFKD 185

Query: 203 GMVEAGIIPYNGFSLDHVMGTKIGGSTFDELGRRHSAADLLSYATPFNIKVAIYATVERI 262
           G++EAG  PYNGF+ DH+ GTKIGG+ FD  G RH+AADLL YA P NI V ++A+V +I
Sbjct: 186 GLLEAGEFPYNGFTYDHIYGTKIGGTIFDRAGHRHTAADLLEYANPGNIVVYLHASVHKI 245

Query: 263 LLASTVKRKPKAIGVLYRDQIGTYHHAML--HARGEVFLSAGAIGSPQLLLLSGIGSRPY 322
           L  +  + +PKA GV+++D  G  H A L  ++  EV LSAGAIGSPQLL+LSGIG   +
Sbjct: 246 LFTTKGRPRPKAYGVIFQDANGVLHKAELEKNSMNEVILSAGAIGSPQLLMLSGIGPAAH 305

Query: 323 LSSWGI-PVAHHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITESGTYLEAASNVI 382
           L++ GI P+    P VG  + DNP N I I +P P+E SLIQV GIT+  +Y+E AS VI
Sbjct: 306 LAAHGIKPLVLDHPMVGQGMGDNPMNAIFIPSPTPVEVSLIQVVGITKFESYIEGASGVI 365

Query: 383 PFTSPPRSFF---------------IRTPSPPVYLTVAT------------------LME 442
              S  R FF                 T SP +     T                  +++
Sbjct: 366 FSYSWTRRFFDGVLNYLNEMQTSRTTSTTSPTLSTQSITDFFNPINPLLNATTRAGLILQ 425

Query: 443 KITGPVASGSMRLASTDVRVNPVVRFNYFGNAVDLERCVNGTRKIGEILRSQAMEGFKFR 502
           KI GP++ G + L +T+   NP VRFNY+    DL+ CV G   I +++ S+A   FK+ 
Sbjct: 426 KIAGPISRGHLELRNTNPDDNPSVRFNYYQEPEDLQICVEGINTIIKVINSKAFSKFKYP 485

Query: 503 D--WFGRKEFRYVGPAYPEKEDEDGLV-LREFCRQSVSTIWHYHGGCVAGKVVDSNYRVI 562
           D    G  +     P          +  LR+FC  +V TIWHYHGGC  G+VVD NYRV+
Sbjct: 486 DATIHGLLDLMLSVPTNLRPRHITSMFNLRQFCIDTVMTIWHYHGGCQVGRVVDKNYRVL 545

Query: 563 GMEALRVVDGSIFGVSPGTNPQATVMMLGRHVGLQIINQR 564
           G+++LRV+DGS F  SPGTNPQATVMMLGR++G +I+ +R
Sbjct: 546 GIDSLRVIDGSTFLKSPGTNPQATVMMLGRYMGQRILQER 576

BLAST of Chy1G021200 vs. TAIR 10
Match: AT5G51950.2 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 446.0 bits (1146), Expect = 4.4e-125
Identity = 251/547 (45.89%), Postives = 331/547 (60.51%), Query Frame = 0

Query: 56  VSNATEFPEVDYYDYIIIGGGTAGCPLAATLSSKFRVLVLERGGVPHGNSNLMSQEGFLT 115
           + +AT  P    +DYIIIGGGT+GC LAATLS    VLVLERGG P+ N      E F T
Sbjct: 1   MKDATLAPMFARFDYIIIGGGTSGCALAATLSQNASVLVLERGGAPYDNPTATDIENFAT 60

Query: 116 TLMDDVHSRNSPVQAFTSEEGVPNARGRILGGSSAINAGFYSRADQAFFKNSQLKWDLAI 175
           TL +   S  S  Q F SE+GV N R R+LGG S +NAGFY+RA   + K ++  W    
Sbjct: 61  TLSNT--SPKSWSQLFISEDGVYNTRARVLGGGSVLNAGFYTRAGDEYVKETE--WKTDE 120

Query: 176 VNQSYEWVEKEIVFKPNLKNWQSAVRDGMVEAGIIPYNGFSLDHVMGTKIGGSTFDELGR 235
           V  +YEWVEK++ F+P +  WQ+A +DG++EAG  PYNGF+ DH+ GTKIGG+ FD  G 
Sbjct: 121 VEAAYEWVEKKVAFQPPVLGWQTAFKDGLLEAGEFPYNGFTYDHIYGTKIGGTIFDRAGH 180

Query: 236 RHSAADLLSYATPFNIKVAIYATVERILLASTVKRKPKAIGVLYRDQIGTYHHAML--HA 295
           RH+AADLL YA P NI V ++A+V +IL  +  + +PKA GV+++D  G  H A L  ++
Sbjct: 181 RHTAADLLEYANPGNIVVYLHASVHKILFTTKGRPRPKAYGVIFQDANGVLHKAELEKNS 240

Query: 296 RGEVFLSAGAIGSPQLLLLSGIGSRPYLSSWGI-PVAHHLPYVGHYLYDNPRNGISILAP 355
             EV LSAGAIGSPQLL+LSGIG   +L++ GI P+    P VG  + DNP N I I +P
Sbjct: 241 MNEVILSAGAIGSPQLLMLSGIGPAAHLAAHGIKPLVLDHPMVGQGMGDNPMNAIFIPSP 300

Query: 356 MPLEQSLIQVAGITESGTYLEAASNVIPFTSPPRSFF---------------IRTPSPPV 415
            P+E SLIQV GIT+  +Y+E AS VI   S  R FF                 T SP +
Sbjct: 301 TPVEVSLIQVVGITKFESYIEGASGVIFSYSWTRRFFDGVLNYLNEMQTSRTTSTTSPTL 360

Query: 416 YLTVAT------------------LMEKITGPVASGSMRLASTDVRVNPVVRFNYFGNAV 475
                T                  +++KI GP++ G + L +T+   NP VRFNY+    
Sbjct: 361 STQSITDFFNPINPLLNATTRAGLILQKIAGPISRGHLELRNTNPDDNPSVRFNYYQEPE 420

Query: 476 DLERCVNGTRKIGEILRSQAMEGFKFRD--WFGRKEFRYVGPAYPEKEDEDGLV-LREFC 535
           DL+ CV G   I +++ S+A   FK+ D    G  +     P          +  LR+FC
Sbjct: 421 DLQICVEGINTIIKVINSKAFSKFKYPDATIHGLLDLMLSVPTNLRPRHITSMFNLRQFC 480

Query: 536 RQSVSTIWHYHGGCVAGKVVDSNYRVIGMEALRVVDGSIFGVSPGTNPQATVMMLGRHVG 564
             +V TIWHYHGGC  G+VVD NYRV+G+++LRV+DGS F  SPGTNPQATVMMLGR++G
Sbjct: 481 IDTVMTIWHYHGGCQVGRVVDKNYRVLGIDSLRVIDGSTFLKSPGTNPQATVMMLGRYMG 540

BLAST of Chy1G021200 vs. TAIR 10
Match: AT1G72970.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 443.7 bits (1140), Expect = 2.2e-124
Identity = 254/595 (42.69%), Postives = 349/595 (58.66%), Query Frame = 0

Query: 19  MASRIFFHSQILTF--LLIFSASSALPNSSQQDPDYLKFVSNATEFP-----------EV 78
           MA ++F  + +L     L  +AS      S+ +P    F+  A+ F            + 
Sbjct: 1   MALKLFLFALLLCLPTSLSSTASKGKEKKSKFNPYRYTFIDKASTFSSSSSSSFSSNGQD 60

Query: 79  DYYDYIIIGGGTAGCPLAATLSSKFRVLVLERGGVPHGNSNLMSQEGFLTTLMDDVHSRN 138
             YDYI+IGGGTAGCPLAATLS  F VLVLERGGVP  N+N+     F   L D   S +
Sbjct: 61  SSYDYIVIGGGTAGCPLAATLSQNFSVLVLERGGVPFTNANVSFLRNFHIGLAD--ISAS 120

Query: 139 SPVQAFTSEEGVPNARGRILGGSSAINAGFYSRADQAFFKNSQLKWDLAIVNQSYEWVEK 198
           S  QAF S +GV NAR R+LGG S INAGFYSRAD AF K +   WD  +V +SY WVE+
Sbjct: 121 SASQAFVSTDGVYNARARVLGGGSCINAGFYSRADAAFVKRA--GWDPKLVKESYPWVER 180

Query: 199 EIVFKPNLKNWQSAVRDGMVEAGIIPYNGFSLDHVMGTKIGGSTFDELGRRHSAADLLSY 258
           EIV +P L  WQ A+RD ++E G+ P+NGF+ DHV GTKIGG+ FD  GRRH+AA+LL+Y
Sbjct: 181 EIVHQPKLTLWQKALRDSLLEVGVRPFNGFTYDHVSGTKIGGTIFDRFGRRHTAAELLAY 240

Query: 259 ATPFNIKVAIYATVERILLASTVKRKPKAIGVLYRDQIGTYHHAMLHAR--GEVFLSAGA 318
           A P  ++V IYATV++I+   T   +P+  GV+++D+ G  H A+L  R   EV LS+GA
Sbjct: 241 ANPQKLRVLIYATVQKIVF-DTSGTRPRVTGVIFKDEKGNQHQALLSNRKGSEVILSSGA 300

Query: 319 IGSPQLLLLSGIGSRPYLSSWGIPVAHHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVA 378
           IGSPQ+L+LSGIG +  L    IPV     +VG  + DNP N I + +  P+EQSLIQ  
Sbjct: 301 IGSPQMLMLSGIGPKKELQRLKIPVVLENEHVGKGMADNPMNTILVPSKAPIEQSLIQTV 360

Query: 379 GITESGTYLEAASNVIPFTSPPRSFFI--------------------RTPSPPVYLT--- 438
           GIT+ G Y+EA++    F   P S                       R  +   Y+T   
Sbjct: 361 GITKMGVYVEASTG---FGQSPESIHTHYGIMSNKNELFSTIPAKQRRPEATQAYITRNK 420

Query: 439 --------VATLMEKITGPVASGSMRLASTDVRVNPVVRFNYFGNAVDLERCVNGTRKIG 498
                    + ++EK+  P++ G + L +T+V  NP V FNYF + VDL+RCV   R + 
Sbjct: 421 YQLHEAFNGSFILEKLAYPISRGHLSLVNTNVDDNPSVTFNYFKHPVDLQRCVEAIRLVS 480

Query: 499 EILRSQAMEGFKFRDWFGRKEFRYVGPA----YPEKEDEDGLVLREFCRQSVSTIWHYHG 558
           +++ S     +   D     +   +          K+  D   + +FC+ +V TIWHYHG
Sbjct: 481 KVVTSNRFLNYTQCDKQNVHKMLSLSVKANINLRPKQLNDTKSMAQFCKDTVVTIWHYHG 540

Query: 559 GCVAGKVVDSNYRVIGMEALRVVDGSIFGVSPGTNPQATVMMLGRHVGLQIINQR 564
           GC+ GKVV  N +V+G++ LRV+DGS F  SPGTNPQAT+MM+GR++G++I+ +R
Sbjct: 541 GCLVGKVVSPNRKVLGVDRLRVIDGSTFDESPGTNPQATMMMMGRYMGVKILRER 587

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SSM27.0e-20864.87(R)-mandelonitrile lyase-like OS=Arabidopsis thaliana OX=3702 GN=At1g73050 PE=2 ... [more]
P527076.4e-14547.90(R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1[more]
P527061.0e-14246.70(R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1[more]
O242432.5e-14146.97(R)-mandelonitrile lyase 1 OS=Prunus dulcis OX=3755 GN=MDL1 PE=1 SV=1[more]
Q945K23.3e-14146.34(R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3CHZ40.0e+0096.68(R)-mandelonitrile lyase-like OS=Cucumis melo OX=3656 GN=LOC103500635 PE=3 SV=1[more]
A0A5D3C0K10.0e+0096.34(R)-mandelonitrile lyase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc... [more]
A0A0A0KCB10.0e+0098.92GMC_OxRdtase_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G447... [more]
A0A6J1JYK61.0e-27081.74(R)-mandelonitrile lyase-like OS=Cucurbita maxima OX=3661 GN=LOC111488606 PE=3 S... [more]
A0A6J1GR643.3e-26981.39(R)-mandelonitrile lyase-like OS=Cucurbita moschata OX=3662 GN=LOC111456379 PE=3... [more]
Match NameE-valueIdentityDescription
XP_008462229.10.096.68PREDICTED: (R)-mandelonitrile lyase-like [Cucumis melo][more]
KAA0059350.10.096.34(R)-mandelonitrile lyase-like [Cucumis melo var. makuwa] >TYK03976.1 (R)-mandelo... [more]
KGN45431.10.098.92hypothetical protein Csa_016192 [Cucumis sativus][more]
XP_011659801.10.099.24(R)-mandelonitrile lyase-like, partial [Cucumis sativus][more]
XP_038899448.10.090.38(R)-mandelonitrile lyase-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT1G73050.14.9e-20964.87Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT1G12570.16.4e-13245.28Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT5G51950.15.2e-12644.66Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT5G51950.24.4e-12545.89Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT1G72970.12.2e-12442.69Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012132Glucose-methanol-choline oxidoreductasePIRSFPIRSF000137Alcohol_oxidasecoord: 51..377
e-value: 6.5E-58
score: 194.8
coord: 367..569
e-value: 1.7E-30
score: 104.3
IPR036188FAD/NAD(P)-binding domain superfamilyGENE3D3.50.50.60coord: 68..552
e-value: 3.6E-167
score: 559.2
IPR036188FAD/NAD(P)-binding domain superfamilySUPERFAMILY51905FAD/NAD(P)-binding domaincoord: 67..563
NoneNo IPR availableGENE3D3.30.410.40coord: 189..502
e-value: 3.6E-167
score: 559.2
NoneNo IPR availablePANTHERPTHR45968OSJNBA0019K04.7 PROTEINcoord: 49..567
NoneNo IPR availablePANTHERPTHR45968:SF2(R)-MANDELONITRILE LYASE-LIKEcoord: 49..567
NoneNo IPR availableSUPERFAMILY54373FAD-linked reductases, C-terminal domaincoord: 339..509
IPR000172Glucose-methanol-choline oxidoreductase, N-terminalPFAMPF00732GMC_oxred_Ncoord: 68..343
e-value: 1.4E-30
score: 106.7
IPR000172Glucose-methanol-choline oxidoreductase, N-terminalPROSITEPS00624GMC_OXRED_2coord: 302..316
IPR007867Glucose-methanol-choline oxidoreductase, C-terminalPFAMPF05199GMC_oxred_Ccoord: 411..554
e-value: 7.0E-29
score: 101.2

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy1G021200.1Chy1G021200.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0016829 lyase activity
molecular_function GO:0016614 oxidoreductase activity, acting on CH-OH group of donors