Chy1G020650 (gene) Cucumber (hystrix) v1

Overview
NameChy1G020650
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionRegulator of Vps4 activity in the MVB pathway protein, putative
LocationchrH01: 25472526 .. 25474453 (-)
RNA-Seq ExpressionChy1G020650
SyntenyChy1G020650
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACGAAGACGCGACTGGATACAACTCGTAAGAAGAAGAATGCGGTTCTTAAGTATTTGAAGAACGACATTGTTGAACTTCTCAAGAGTCGTCTCGATTACAATGCTTACAACAGGGTTAGTTCATCTTCTTGACGGATTCTGTTCTTAAGATGGGTTTTAGATTCTTGATCTCTGGTTAATTTCGTCTTTATTGGCTTTAGTTCTTTTATGTGGTTTCGCTTTATGTGTTACTTTTAAATACCACTGAACAGAGCTTTTGTCCCCCCCTTTACACATTCTTCTTTTACCTTGAGTTATTGTCAAAGAGAAATTTGGGCTTATCTTCTCTGGTTTTGGTCGCTAATCTACTTTTGTTACTGGTGAAATAGGCTGAAGGGTTTCTTGTTGAGCGAAATGTCTTGAGATGTTATGAACTGATTGATGAGTTTTGTGGCACAATCTCCAATCAAATTTCTGTCCTGAACAAGGAGAGGTTGATAGATACCATGGATTTTTTTCATTACATTTCTCTTTCTCAGGCTTGAATCCGTTGAGATGGATTTAAATGTTGTTCCTATGTTTTTGTTTCTCTTCAGTGAATGTCCAGATGAGTGCAAAGAAGCGGTTGCAACGTTAATTTACGCTGCAGCGAGATTCGCTGACTTGCCTGAATTGCGAGAGCTTCGAAATCTCTTTACTGAGAAATATGGAAGTTCCTTCGGATCATTTACTAATAAAGAGGTAGTTCTTAGTAGCTCTTATCCATGAATGAAAACTCAACTGTCTTGCTTGCGGGAGTTTCTTCCTTTTTATCATCCTTAAAAGACTGTTTGGTGTTCTTGCTTTTCGCTTTAGTTTATCGAAAATTCGAGGACAACAACTCAAACTAAAGAGATGAAGATTCAGCTGCTCCAAGAGATAGCTCAGGAAACAGCCATTGATTGGAATTCCAAGGCTCTTGAACAGCAGCTGTATACTCCTCCTCCCGAAAACGAAGTAAGTTTGTTTGTATCTAATTTTATCTGTTTTCAATTGAGTTGAGTGAATGCTAAAGTAATTCGAGTGAGTTTCTGTTTTGTGTCGATAATCTCACTTCATTCTATTGATGGTTGTATCTGCTGCAATAATGGTTATTCTTTTCACAGCTTGATGGTGAACGGAGTGGAGCCACCAAAAGAAACAAAACGAAGGTTGTTTCCGTTCCAGTGTATGAAAAAAAGGCAAACTCACCAAGAAACAAGAACAATAGTGATAATGAATCCATCTTTGATAGTAGAAGTGAAGGCAACACAACCGAAACCTCGACAGGAGATAGTACTGATCAAGATGTTCATAAAGGCGTTTTGGGGGATGAAGTAGATCAGAAACCTTTCAACCGTAGATTTGTCCAACCTCCCTACCTTAAAACAAAACCCATCAAAACAGAAGCAAATGGAGAGGAATTACCTCGAAAAGTAACTATCGAAAACGATGAAAGTAATCCAAAGTCACCCACAGAAGAGAAGCCGAAACCGAGGTCCGTGAGGAGAAGAATTGTGAAACCACAGCCTGCAAGGGACATCAACATTGACGACGTTGGGAGTTCCACAATCGATGTAACGAAAAAGATTAGCTCAAGTCGAAACAAAGGTAAAGAAACCATGATAGGGGAAGAGAAAGGTGCAAGGGATGATGAAGAAAGGGTATTAGATGGGCTTTTAATGCAATATAGTACGAAGAAAACAAATCAAGAATCAAAAAGTAGAGGAAAAAGCAACCTTAAACCACAAAGACAGCAAGAGAAAGACAATATTGAACATCAAAGGCCCACCTCCAGAGCAGTATCACTTCCCCCAGACCAAAATGAGCCAATGAAAAAGCATACTAGAACAAATTCCTTCGTTCATCCTAAGCTCCCAGAATACGACCAACTCGCAGCTCGGATTGCAGCACTGAAAGAGAAGTAA

mRNA sequence

ATGACGAAGACGCGACTGGATACAACTCGTAAGAAGAAGAATGCGGTTCTTAAGTATTTGAAGAACGACATTGTTGAACTTCTCAAGAGTCGTCTCGATTACAATGCTTACAACAGGGCTGAAGGGTTTCTTGTTGAGCGAAATGTCTTGAGATGTTATGAACTGATTGATGAGTTTTGTGGCACAATCTCCAATCAAATTTCTGTCCTGAACAAGGAGAGTGAATGTCCAGATGAGTGCAAAGAAGCGGTTGCAACGTTAATTTACGCTGCAGCGAGATTCGCTGACTTGCCTGAATTGCGAGAGCTTCGAAATCTCTTTACTGAGAAATATGGAAGTTCCTTCGGATCATTTACTAATAAAGAGTTTATCGAAAATTCGAGGACAACAACTCAAACTAAAGAGATGAAGATTCAGCTGCTCCAAGAGATAGCTCAGGAAACAGCCATTGATTGGAATTCCAAGGCTCTTGAACAGCAGCTGTATACTCCTCCTCCCGAAAACGAACTTGATGGTGAACGGAGTGGAGCCACCAAAAGAAACAAAACGAAGGTTGTTTCCGTTCCAGTGTATGAAAAAAAGGCAAACTCACCAAGAAACAAGAACAATAGTGATAATGAATCCATCTTTGATAGTAGAAGTGAAGGCAACACAACCGAAACCTCGACAGGAGATAGTACTGATCAAGATGTTCATAAAGGCGTTTTGGGGGATGAAGTAGATCAGAAACCTTTCAACCGTAGATTTGTCCAACCTCCCTACCTTAAAACAAAACCCATCAAAACAGAAGCAAATGGAGAGGAATTACCTCGAAAAGTAACTATCGAAAACGATGAAAGTAATCCAAAGTCACCCACAGAAGAGAAGCCGAAACCGAGGTCCGTGAGGAGAAGAATTGTGAAACCACAGCCTGCAAGGGACATCAACATTGACGACGTTGGGAGTTCCACAATCGATGTAACGAAAAAGATTAGCTCAAGTCGAAACAAAGGTAAAGAAACCATGATAGGGGAAGAGAAAGGTGCAAGGGATGATGAAGAAAGGGTATTAGATGGGCTTTTAATGCAATATAGTACGAAGAAAACAAATCAAGAATCAAAAAGTAGAGGAAAAAGCAACCTTAAACCACAAAGACAGCAAGAGAAAGACAATATTGAACATCAAAGGCCCACCTCCAGAGCAGTATCACTTCCCCCAGACCAAAATGAGCCAATGAAAAAGCATACTAGAACAAATTCCTTCGTTCATCCTAAGCTCCCAGAATACGACCAACTCGCAGCTCGGATTGCAGCACTGAAAGAGAAGTAA

Coding sequence (CDS)

ATGACGAAGACGCGACTGGATACAACTCGTAAGAAGAAGAATGCGGTTCTTAAGTATTTGAAGAACGACATTGTTGAACTTCTCAAGAGTCGTCTCGATTACAATGCTTACAACAGGGCTGAAGGGTTTCTTGTTGAGCGAAATGTCTTGAGATGTTATGAACTGATTGATGAGTTTTGTGGCACAATCTCCAATCAAATTTCTGTCCTGAACAAGGAGAGTGAATGTCCAGATGAGTGCAAAGAAGCGGTTGCAACGTTAATTTACGCTGCAGCGAGATTCGCTGACTTGCCTGAATTGCGAGAGCTTCGAAATCTCTTTACTGAGAAATATGGAAGTTCCTTCGGATCATTTACTAATAAAGAGTTTATCGAAAATTCGAGGACAACAACTCAAACTAAAGAGATGAAGATTCAGCTGCTCCAAGAGATAGCTCAGGAAACAGCCATTGATTGGAATTCCAAGGCTCTTGAACAGCAGCTGTATACTCCTCCTCCCGAAAACGAACTTGATGGTGAACGGAGTGGAGCCACCAAAAGAAACAAAACGAAGGTTGTTTCCGTTCCAGTGTATGAAAAAAAGGCAAACTCACCAAGAAACAAGAACAATAGTGATAATGAATCCATCTTTGATAGTAGAAGTGAAGGCAACACAACCGAAACCTCGACAGGAGATAGTACTGATCAAGATGTTCATAAAGGCGTTTTGGGGGATGAAGTAGATCAGAAACCTTTCAACCGTAGATTTGTCCAACCTCCCTACCTTAAAACAAAACCCATCAAAACAGAAGCAAATGGAGAGGAATTACCTCGAAAAGTAACTATCGAAAACGATGAAAGTAATCCAAAGTCACCCACAGAAGAGAAGCCGAAACCGAGGTCCGTGAGGAGAAGAATTGTGAAACCACAGCCTGCAAGGGACATCAACATTGACGACGTTGGGAGTTCCACAATCGATGTAACGAAAAAGATTAGCTCAAGTCGAAACAAAGGTAAAGAAACCATGATAGGGGAAGAGAAAGGTGCAAGGGATGATGAAGAAAGGGTATTAGATGGGCTTTTAATGCAATATAGTACGAAGAAAACAAATCAAGAATCAAAAAGTAGAGGAAAAAGCAACCTTAAACCACAAAGACAGCAAGAGAAAGACAATATTGAACATCAAAGGCCCACCTCCAGAGCAGTATCACTTCCCCCAGACCAAAATGAGCCAATGAAAAAGCATACTAGAACAAATTCCTTCGTTCATCCTAAGCTCCCAGAATACGACCAACTCGCAGCTCGGATTGCAGCACTGAAAGAGAAGTAA

Protein sequence

MTKTRLDTTRKKKNAVLKYLKNDIVELLKSRLDYNAYNRAEGFLVERNVLRCYELIDEFCGTISNQISVLNKESECPDECKEAVATLIYAAARFADLPELRELRNLFTEKYGSSFGSFTNKEFIENSRTTTQTKEMKIQLLQEIAQETAIDWNSKALEQQLYTPPPENELDGERSGATKRNKTKVVSVPVYEKKANSPRNKNNSDNESIFDSRSEGNTTETSTGDSTDQDVHKGVLGDEVDQKPFNRRFVQPPYLKTKPIKTEANGEELPRKVTIENDESNPKSPTEEKPKPRSVRRRIVKPQPARDINIDDVGSSTIDVTKKISSSRNKGKETMIGEEKGARDDEERVLDGLLMQYSTKKTNQESKSRGKSNLKPQRQQEKDNIEHQRPTSRAVSLPPDQNEPMKKHTRTNSFVHPKLPEYDQLAARIAALKEK*
Homology
BLAST of Chy1G020650 vs. ExPASy Swiss-Prot
Match: Q54I39 (IST1-like protein OS=Dictyostelium discoideum OX=44689 GN=DDB_G0289029 PE=3 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 7.5e-13
Identity = 44/172 (25.58%), Postives = 87/172 (50.58%), Query Frame = 0

Query: 4   TRLDTTRKKKNAVLKYLKNDIVELLKSRLDYNAYNRAEGFLVERNVLRCYELIDEFCGTI 63
           +R+   + KK  +++  K ++ ELL+ + + +A  R E  + +  ++ C+++I+  C  +
Sbjct: 22  SRIQILKNKKANIVRDEKRNVAELLRKKNEESARIRVETIIRDEYLIECFQIIEVLCELL 81

Query: 64  SNQISVLNKESECPDECKEAVATLIYAAARFADLPELRELRNLFTEKYGSSF-------- 123
             +I+++N  +E P E KE++ TL+Y++ R   +PEL +++N    KYG           
Sbjct: 82  HARINLINATTEMPLEMKESIFTLVYSSQRI-QIPELEQIKNQLKAKYGKGLENEANCHC 141

Query: 124 GSFTNKEFIENSRTTTQTKEMKIQLLQEIAQETAIDWNSKALEQQLYTPPPE 168
            +  N + +      T    +  Q L EIA++  +DW         Y PPP+
Sbjct: 142 STHVNPKIVHKLSYATPDPSIIFQTLSEIAEKFNVDWCGSD-----YPPPPQ 187

BLAST of Chy1G020650 vs. ExPASy TrEMBL
Match: A0A0A0K926 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G446760 PE=3 SV=1)

HSP 1 Score: 815.5 bits (2105), Expect = 1.1e-232
Identity = 426/435 (97.93%), Postives = 428/435 (98.39%), Query Frame = 0

Query: 1   MTKTRLDTTRKKKNAVLKYLKNDIVELLKSRLDYNAYNRAEGFLVERNVLRCYELIDEFC 60
           MTKTRLDTTRKKKNAVLKYLKNDIVELLKSRLDYNAYNRAEGFLVERNVLRCYELIDEFC
Sbjct: 20  MTKTRLDTTRKKKNAVLKYLKNDIVELLKSRLDYNAYNRAEGFLVERNVLRCYELIDEFC 79

Query: 61  GTISNQISVLNKESECPDECKEAVATLIYAAARFADLPELRELRNLFTEKYGSSFGSFTN 120
           GTISNQI VLNKESECPDECKE+VATLIYAAARFADLPELRELRNLFTEKYGSSFGSFTN
Sbjct: 80  GTISNQIPVLNKESECPDECKESVATLIYAAARFADLPELRELRNLFTEKYGSSFGSFTN 139

Query: 121 KEFIENSRTTTQTKEMKIQLLQEIAQETAIDWNSKALEQQLYTPPPENELDGERSGATKR 180
           KEFIE SRTTTQTKEMKIQLLQEIAQETAIDWNSKALEQQLYTPPPENELDGERSGATKR
Sbjct: 140 KEFIEKSRTTTQTKEMKIQLLQEIAQETAIDWNSKALEQQLYTPPPENELDGERSGATKR 199

Query: 181 NKTKVVSVPVYEKKANSPRNKNNSDNESIFDSRSEGNTTETSTGDSTDQDVHKGVLGDEV 240
           NKTKVVSVPVYEKKANSPRNKNNSDNESIFDSRSEGNTTETSTGDSTDQDVHKGV GDEV
Sbjct: 200 NKTKVVSVPVYEKKANSPRNKNNSDNESIFDSRSEGNTTETSTGDSTDQDVHKGVSGDEV 259

Query: 241 DQKPFNRRFVQPPYLKTKPIKTEANGEELPRKVTIENDESNPKSPTEEKPKPRSVRRRIV 300
           DQKPFNRRFVQPPYLKTKPIKTEAN EELPRKVTIENDESNPKSPTEEKPKPRSVRRRIV
Sbjct: 260 DQKPFNRRFVQPPYLKTKPIKTEANAEELPRKVTIENDESNPKSPTEEKPKPRSVRRRIV 319

Query: 301 KPQPARDINIDDVGSSTIDVTKKISSSRNKGKETMIGEEKGARDDEERVLDGLLMQYSTK 360
           KPQPARDINIDDVGSST+DVTKKISS RNKGKETMIGEEKGARDDEERVLDGLLMQYS K
Sbjct: 320 KPQPARDINIDDVGSSTVDVTKKISSIRNKGKETMIGEEKGARDDEERVLDGLLMQYSKK 379

Query: 361 KTNQESKSRGKSNLKPQRQQEKDNIEHQRPTSRAVSLPPDQNEPMKKHTRTNSFVHPKLP 420
           KTNQESKSRGKSNLKPQRQQEKDNIEHQRPTSRAVS PPDQNEPMKKHTRTNSFVHPKLP
Sbjct: 380 KTNQESKSRGKSNLKPQRQQEKDNIEHQRPTSRAVSFPPDQNEPMKKHTRTNSFVHPKLP 439

Query: 421 EYDQLAARIAALKEK 436
           EYDQLAARIAALKEK
Sbjct: 440 EYDQLAARIAALKEK 454

BLAST of Chy1G020650 vs. ExPASy TrEMBL
Match: A0A5D3BX00 (Putative Regulator of Vps4 activity in the MVB pathway protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G002360 PE=3 SV=1)

HSP 1 Score: 774.6 bits (1999), Expect = 2.1e-220
Identity = 414/437 (94.74%), Postives = 420/437 (96.11%), Query Frame = 0

Query: 1   MTKTRLDTTRKKKNAVLKYLKNDIVELLKSRLDYNAYNRAEGFLVERNVLRCYELIDEFC 60
           MTKTRLDT RKKKNAVLKYLKNDIVELLKSRLDYNA+NRAEGFLVERNVLRCYELIDEFC
Sbjct: 1   MTKTRLDTIRKKKNAVLKYLKNDIVELLKSRLDYNAFNRAEGFLVERNVLRCYELIDEFC 60

Query: 61  GTISNQISVLNKESECPDECKEAVATLIYAAARFADLPELRELRNLFTEKYGSSFGSFTN 120
           GTISNQI VLNKESECPDECKEAVATLIYAAARFADLPELRELRNLFTEKYGSSFGSFTN
Sbjct: 61  GTISNQIPVLNKESECPDECKEAVATLIYAAARFADLPELRELRNLFTEKYGSSFGSFTN 120

Query: 121 KEFIENSRTTTQTKEMKIQLLQEIAQETAIDWNSKALEQQLYTPPPENELDGERSGATKR 180
           KEFIE SR TTQTKEMKIQLLQEIAQETAIDWNSKALEQQLYTPPP+NELDGERSGATKR
Sbjct: 121 KEFIEKSRATTQTKEMKIQLLQEIAQETAIDWNSKALEQQLYTPPPQNELDGERSGATKR 180

Query: 181 NKTKVVSVPVYEKKANSPRNKNNSDNESIFDSRSEGNTTETSTGDSTDQDVHKGVLGDEV 240
           NKTKVVSVPVYE+KANSPRNKNNSDNESIFDSRSEGNTTETSTGDSTDQD+HKGV  DEV
Sbjct: 181 NKTKVVSVPVYERKANSPRNKNNSDNESIFDSRSEGNTTETSTGDSTDQDIHKGVSEDEV 240

Query: 241 -DQKPFNRRFVQPPYLKTKPIKTEANGEELPRKVTIENDES-NPKSPTEEKPKPRSVRRR 300
            DQKPFN RFVQPPYLKTKPIKTEAN +E PRKVTIENDES NPKSPTEEKPKPRSVRRR
Sbjct: 241 EDQKPFNHRFVQPPYLKTKPIKTEANEKE-PRKVTIENDESNNPKSPTEEKPKPRSVRRR 300

Query: 301 IVKPQPARDINIDDVGSSTIDVTKKISSSRNKGKETMIGEEKGARDDEERVLDGLLMQYS 360
            VKPQPARDINIDDVGSSTIDVT KISSSRNKGKETMIGEEKGARDDEERVLDGLLMQYS
Sbjct: 301 NVKPQPARDINIDDVGSSTIDVTTKISSSRNKGKETMIGEEKGARDDEERVLDGLLMQYS 360

Query: 361 TKKTNQESKSRGKSNLKPQRQQEKDNIEHQRPTSRAVSLPPDQNEPMKKHTRTNSFVHPK 420
            KKTNQESKSRGKSNLKPQRQQEKDNIEHQRPT+RAVSLP DQNE  KKHTRTNSFVHPK
Sbjct: 361 KKKTNQESKSRGKSNLKPQRQQEKDNIEHQRPTTRAVSLPTDQNESTKKHTRTNSFVHPK 420

Query: 421 LPEYDQLAARIAALKEK 436
           LPEYDQLAARIAALKEK
Sbjct: 421 LPEYDQLAARIAALKEK 436

BLAST of Chy1G020650 vs. ExPASy TrEMBL
Match: A0A1S3CGT5 (uncharacterized protein LOC103500577 OS=Cucumis melo OX=3656 GN=LOC103500577 PE=3 SV=1)

HSP 1 Score: 774.6 bits (1999), Expect = 2.1e-220
Identity = 414/437 (94.74%), Postives = 420/437 (96.11%), Query Frame = 0

Query: 1   MTKTRLDTTRKKKNAVLKYLKNDIVELLKSRLDYNAYNRAEGFLVERNVLRCYELIDEFC 60
           MTKTRLDT RKKKNAVLKYLKNDIVELLKSRLDYNA+NRAEGFLVERNVLRCYELIDEFC
Sbjct: 20  MTKTRLDTIRKKKNAVLKYLKNDIVELLKSRLDYNAFNRAEGFLVERNVLRCYELIDEFC 79

Query: 61  GTISNQISVLNKESECPDECKEAVATLIYAAARFADLPELRELRNLFTEKYGSSFGSFTN 120
           GTISNQI VLNKESECPDECKEAVATLIYAAARFADLPELRELRNLFTEKYGSSFGSFTN
Sbjct: 80  GTISNQIPVLNKESECPDECKEAVATLIYAAARFADLPELRELRNLFTEKYGSSFGSFTN 139

Query: 121 KEFIENSRTTTQTKEMKIQLLQEIAQETAIDWNSKALEQQLYTPPPENELDGERSGATKR 180
           KEFIE SR TTQTKEMKIQLLQEIAQETAIDWNSKALEQQLYTPPP+NELDGERSGATKR
Sbjct: 140 KEFIEKSRATTQTKEMKIQLLQEIAQETAIDWNSKALEQQLYTPPPQNELDGERSGATKR 199

Query: 181 NKTKVVSVPVYEKKANSPRNKNNSDNESIFDSRSEGNTTETSTGDSTDQDVHKGVLGDEV 240
           NKTKVVSVPVYE+KANSPRNKNNSDNESIFDSRSEGNTTETSTGDSTDQD+HKGV  DEV
Sbjct: 200 NKTKVVSVPVYERKANSPRNKNNSDNESIFDSRSEGNTTETSTGDSTDQDIHKGVSEDEV 259

Query: 241 -DQKPFNRRFVQPPYLKTKPIKTEANGEELPRKVTIENDES-NPKSPTEEKPKPRSVRRR 300
            DQKPFN RFVQPPYLKTKPIKTEAN +E PRKVTIENDES NPKSPTEEKPKPRSVRRR
Sbjct: 260 EDQKPFNHRFVQPPYLKTKPIKTEANEKE-PRKVTIENDESNNPKSPTEEKPKPRSVRRR 319

Query: 301 IVKPQPARDINIDDVGSSTIDVTKKISSSRNKGKETMIGEEKGARDDEERVLDGLLMQYS 360
            VKPQPARDINIDDVGSSTIDVT KISSSRNKGKETMIGEEKGARDDEERVLDGLLMQYS
Sbjct: 320 NVKPQPARDINIDDVGSSTIDVTTKISSSRNKGKETMIGEEKGARDDEERVLDGLLMQYS 379

Query: 361 TKKTNQESKSRGKSNLKPQRQQEKDNIEHQRPTSRAVSLPPDQNEPMKKHTRTNSFVHPK 420
            KKTNQESKSRGKSNLKPQRQQEKDNIEHQRPT+RAVSLP DQNE  KKHTRTNSFVHPK
Sbjct: 380 KKKTNQESKSRGKSNLKPQRQQEKDNIEHQRPTTRAVSLPTDQNESTKKHTRTNSFVHPK 439

Query: 421 LPEYDQLAARIAALKEK 436
           LPEYDQLAARIAALKEK
Sbjct: 440 LPEYDQLAARIAALKEK 455

BLAST of Chy1G020650 vs. ExPASy TrEMBL
Match: A0A6J1GMM1 (uncharacterized protein LOC111455825 OS=Cucurbita moschata OX=3662 GN=LOC111455825 PE=3 SV=1)

HSP 1 Score: 559.7 bits (1441), Expect = 1.1e-155
Identity = 317/441 (71.88%), Postives = 355/441 (80.50%), Query Frame = 0

Query: 1   MTKTRLDTTRKKKNAVLKYLKNDIVELLKSRLDYNAYNRAEGFLVERNVLRCYELIDEFC 60
           MTKTRLDT RKKK  VLK+LKNDIVELLKSRLDYNAYNRAEGFLVE+NVLRCY+LIDEFC
Sbjct: 20  MTKTRLDTIRKKKKVVLKFLKNDIVELLKSRLDYNAYNRAEGFLVEQNVLRCYDLIDEFC 79

Query: 61  GTISNQISVLNKESECPDECKEAVATLIYAAARFADLPELRELRNLFTEKYGSSFGSFTN 120
           GTI   ISVLNKESECPDECKEAVA+LIYAAARF+DLPELR LR+LFTE+YG+SFGSFTN
Sbjct: 80  GTIFKHISVLNKESECPDECKEAVASLIYAAARFSDLPELRVLRSLFTERYGTSFGSFTN 139

Query: 121 KEFIENSRTTTQTKEMKIQLLQEIAQETAIDWNSKALEQQLYTPPPENELDGERSGATKR 180
           KE +E  R   QTKE K+QLLQEIAQE+AIDWNSKALEQQLY  PP+NELD ERS +T R
Sbjct: 140 KELVEKLRAVAQTKETKLQLLQEIAQESAIDWNSKALEQQLYV-PPQNELDCERSTSTCR 199

Query: 181 NKTKVVSVPVYEKKANSPRNKNNSDNESIFDSRSEGNTTETSTGD-STDQDVHKGVLG-D 240
           +K+K+VSVPVY +  NSPR K+NSD++SIFDSRSEGNTTETS GD STDQDVHKGV+  D
Sbjct: 200 SKSKIVSVPVYGRTTNSPRKKDNSDDDSIFDSRSEGNTTETSMGDSSTDQDVHKGVVSED 259

Query: 241 EV-DQKPFNRRFVQPPYLKTKPIKTEANGEELPRKVTIENDESNPKSPTEEKPKPRSVRR 300
           EV DQKPF  RF+ PPYLKTKP K E N EE P+  T EN  ++PK+  EEKPKPRSVRR
Sbjct: 260 EVEDQKPFYLRFITPPYLKTKPTKKEENVEE-PQNDTEEN-RNDPKTAIEEKPKPRSVRR 319

Query: 301 RIVKPQPARDINIDDVGSSTIDVTKKISSSRNKGKETMIGEEKGARDDEERVLDGLLMQY 360
           R  KPQPAR INIDD G S  D   KISSSRNKGKE M GEEKG  DDEER+LDGLL  Y
Sbjct: 320 RHAKPQPARSINIDDAGGSAADGAGKISSSRNKGKEAMTGEEKGVEDDEERMLDGLLKLY 379

Query: 361 STKKTNQESKSRGKSNLKPQRQQEKDNIEHQRPTSRAVSLPPDQN---EPMKKHTRTNSF 420
           S KKT QE     K N +PQR +     +   PT +++SLPP +    EP+KKH R NS+
Sbjct: 380 SKKKTRQED----KDNAEPQRPKNTRTEDAYVPT-KSLSLPPPRTEPIEPVKKHARANSY 439

Query: 421 VHPKLPEYDQLAARIAALKEK 436
           VHPKLP+YDQLAAR A+LKEK
Sbjct: 440 VHPKLPDYDQLAARFASLKEK 452

BLAST of Chy1G020650 vs. ExPASy TrEMBL
Match: A0A6J1JV69 (uncharacterized protein LOC111488629 OS=Cucurbita maxima OX=3661 GN=LOC111488629 PE=3 SV=1)

HSP 1 Score: 553.1 bits (1424), Expect = 1.0e-153
Identity = 313/441 (70.98%), Postives = 353/441 (80.05%), Query Frame = 0

Query: 1   MTKTRLDTTRKKKNAVLKYLKNDIVELLKSRLDYNAYNRAEGFLVERNVLRCYELIDEFC 60
           MTKTRLDT RKKK  VLK+LKNDIVELLKSRLDYNAYNRAEGFLVE+NVLRCY+LIDEFC
Sbjct: 20  MTKTRLDTIRKKKKVVLKFLKNDIVELLKSRLDYNAYNRAEGFLVEQNVLRCYDLIDEFC 79

Query: 61  GTISNQISVLNKESECPDECKEAVATLIYAAARFADLPELRELRNLFTEKYGSSFGSFTN 120
           GTI N ISVLNKESECPDECKEAVA+LIYAAARF+DLPELR LR+LFTE+YG+SFGSFTN
Sbjct: 80  GTIFNHISVLNKESECPDECKEAVASLIYAAARFSDLPELRVLRSLFTERYGTSFGSFTN 139

Query: 121 KEFIENSRTTTQTKEMKIQLLQEIAQETAIDWNSKALEQQLYTPPPENELDGERSGATKR 180
           KE +E  R   QTKE K+QLLQEIAQE+AIDWNSKALEQQLY  PP+NEL  ERS +T R
Sbjct: 140 KELVEKLRAVAQTKETKLQLLQEIAQESAIDWNSKALEQQLYV-PPQNELVRERSTSTNR 199

Query: 181 NKTKVVSVPVYEKKANSPRNKNNSDNESIFDSRSEGNTTETSTGD-STDQDVHKGVLG-D 240
           +K+K+VSVPVY +  NSP+ K+NSD++SIFDSRSEGNTTETS GD STDQDVHKG++  D
Sbjct: 200 SKSKIVSVPVYGRTTNSPKKKDNSDDDSIFDSRSEGNTTETSMGDSSTDQDVHKGIVSED 259

Query: 241 EV-DQKPFNRRFVQPPYLKTKPIKTEANGEELPRKVTIENDESNPKSPTEEKPKPRSVRR 300
           EV DQKPF  RF+ PPYLKTKP K E N EE P+ VT E   +NPK+ TEEKPKPRSVRR
Sbjct: 260 EVEDQKPFYLRFITPPYLKTKPTKKEENVEE-PQNVT-EEKRNNPKTTTEEKPKPRSVRR 319

Query: 301 RIVKPQPARDINIDDVGSSTIDVTKKISSSRNKGKETMIGEEKGARDDEERVLDGLLMQY 360
           R  KPQPAR INIDD G S  D   KISS +NKGKE M GEEKG  DDEER+LDGLL  Y
Sbjct: 320 RHAKPQPARSINIDDSGGSAADGAGKISSGQNKGKEAMTGEEKGEEDDEERMLDGLLKIY 379

Query: 361 STKKTNQESKSRGKSNLKPQRQQEKDNIEHQRPTSRAVSLPPDQN---EPMKKHTRTNSF 420
           S K T QE     K N +PQR +     +   P ++++SLP  +    EP+KKH R NS+
Sbjct: 380 SKKNTRQED----KDNAEPQRPKNTRTEDAYVP-AKSLSLPTARTEPIEPVKKHARANSY 439

Query: 421 VHPKLPEYDQLAARIAALKEK 436
           VHPKLP+YDQLAAR AALKEK
Sbjct: 440 VHPKLPDYDQLAARFAALKEK 452

BLAST of Chy1G020650 vs. NCBI nr
Match: XP_004141848.1 (uncharacterized protein LOC101220202 [Cucumis sativus] >KGN45359.1 hypothetical protein Csa_015927 [Cucumis sativus])

HSP 1 Score: 820 bits (2118), Expect = 1.59e-298
Identity = 426/435 (97.93%), Postives = 428/435 (98.39%), Query Frame = 0

Query: 1   MTKTRLDTTRKKKNAVLKYLKNDIVELLKSRLDYNAYNRAEGFLVERNVLRCYELIDEFC 60
           MTKTRLDTTRKKKNAVLKYLKNDIVELLKSRLDYNAYNRAEGFLVERNVLRCYELIDEFC
Sbjct: 20  MTKTRLDTTRKKKNAVLKYLKNDIVELLKSRLDYNAYNRAEGFLVERNVLRCYELIDEFC 79

Query: 61  GTISNQISVLNKESECPDECKEAVATLIYAAARFADLPELRELRNLFTEKYGSSFGSFTN 120
           GTISNQI VLNKESECPDECKE+VATLIYAAARFADLPELRELRNLFTEKYGSSFGSFTN
Sbjct: 80  GTISNQIPVLNKESECPDECKESVATLIYAAARFADLPELRELRNLFTEKYGSSFGSFTN 139

Query: 121 KEFIENSRTTTQTKEMKIQLLQEIAQETAIDWNSKALEQQLYTPPPENELDGERSGATKR 180
           KEFIE SRTTTQTKEMKIQLLQEIAQETAIDWNSKALEQQLYTPPPENELDGERSGATKR
Sbjct: 140 KEFIEKSRTTTQTKEMKIQLLQEIAQETAIDWNSKALEQQLYTPPPENELDGERSGATKR 199

Query: 181 NKTKVVSVPVYEKKANSPRNKNNSDNESIFDSRSEGNTTETSTGDSTDQDVHKGVLGDEV 240
           NKTKVVSVPVYEKKANSPRNKNNSDNESIFDSRSEGNTTETSTGDSTDQDVHKGV GDEV
Sbjct: 200 NKTKVVSVPVYEKKANSPRNKNNSDNESIFDSRSEGNTTETSTGDSTDQDVHKGVSGDEV 259

Query: 241 DQKPFNRRFVQPPYLKTKPIKTEANGEELPRKVTIENDESNPKSPTEEKPKPRSVRRRIV 300
           DQKPFNRRFVQPPYLKTKPIKTEAN EELPRKVTIENDESNPKSPTEEKPKPRSVRRRIV
Sbjct: 260 DQKPFNRRFVQPPYLKTKPIKTEANAEELPRKVTIENDESNPKSPTEEKPKPRSVRRRIV 319

Query: 301 KPQPARDINIDDVGSSTIDVTKKISSSRNKGKETMIGEEKGARDDEERVLDGLLMQYSTK 360
           KPQPARDINIDDVGSST+DVTKKISS RNKGKETMIGEEKGARDDEERVLDGLLMQYS K
Sbjct: 320 KPQPARDINIDDVGSSTVDVTKKISSIRNKGKETMIGEEKGARDDEERVLDGLLMQYSKK 379

Query: 361 KTNQESKSRGKSNLKPQRQQEKDNIEHQRPTSRAVSLPPDQNEPMKKHTRTNSFVHPKLP 420
           KTNQESKSRGKSNLKPQRQQEKDNIEHQRPTSRAVS PPDQNEPMKKHTRTNSFVHPKLP
Sbjct: 380 KTNQESKSRGKSNLKPQRQQEKDNIEHQRPTSRAVSFPPDQNEPMKKHTRTNSFVHPKLP 439

Query: 421 EYDQLAARIAALKEK 435
           EYDQLAARIAALKEK
Sbjct: 440 EYDQLAARIAALKEK 454

BLAST of Chy1G020650 vs. NCBI nr
Match: KAA0059291.1 (putative Regulator of Vps4 activity in the MVB pathway protein [Cucumis melo var. makuwa] >TYK04037.1 putative Regulator of Vps4 activity in the MVB pathway protein [Cucumis melo var. makuwa])

HSP 1 Score: 779 bits (2012), Expect = 1.14e-282
Identity = 414/437 (94.74%), Postives = 420/437 (96.11%), Query Frame = 0

Query: 1   MTKTRLDTTRKKKNAVLKYLKNDIVELLKSRLDYNAYNRAEGFLVERNVLRCYELIDEFC 60
           MTKTRLDT RKKKNAVLKYLKNDIVELLKSRLDYNA+NRAEGFLVERNVLRCYELIDEFC
Sbjct: 1   MTKTRLDTIRKKKNAVLKYLKNDIVELLKSRLDYNAFNRAEGFLVERNVLRCYELIDEFC 60

Query: 61  GTISNQISVLNKESECPDECKEAVATLIYAAARFADLPELRELRNLFTEKYGSSFGSFTN 120
           GTISNQI VLNKESECPDECKEAVATLIYAAARFADLPELRELRNLFTEKYGSSFGSFTN
Sbjct: 61  GTISNQIPVLNKESECPDECKEAVATLIYAAARFADLPELRELRNLFTEKYGSSFGSFTN 120

Query: 121 KEFIENSRTTTQTKEMKIQLLQEIAQETAIDWNSKALEQQLYTPPPENELDGERSGATKR 180
           KEFIE SR TTQTKEMKIQLLQEIAQETAIDWNSKALEQQLYTPPP+NELDGERSGATKR
Sbjct: 121 KEFIEKSRATTQTKEMKIQLLQEIAQETAIDWNSKALEQQLYTPPPQNELDGERSGATKR 180

Query: 181 NKTKVVSVPVYEKKANSPRNKNNSDNESIFDSRSEGNTTETSTGDSTDQDVHKGVLGDEV 240
           NKTKVVSVPVYE+KANSPRNKNNSDNESIFDSRSEGNTTETSTGDSTDQD+HKGV  DEV
Sbjct: 181 NKTKVVSVPVYERKANSPRNKNNSDNESIFDSRSEGNTTETSTGDSTDQDIHKGVSEDEV 240

Query: 241 -DQKPFNRRFVQPPYLKTKPIKTEANGEELPRKVTIENDESN-PKSPTEEKPKPRSVRRR 300
            DQKPFN RFVQPPYLKTKPIKTEAN +E PRKVTIENDESN PKSPTEEKPKPRSVRRR
Sbjct: 241 EDQKPFNHRFVQPPYLKTKPIKTEANEKE-PRKVTIENDESNNPKSPTEEKPKPRSVRRR 300

Query: 301 IVKPQPARDINIDDVGSSTIDVTKKISSSRNKGKETMIGEEKGARDDEERVLDGLLMQYS 360
            VKPQPARDINIDDVGSSTIDVT KISSSRNKGKETMIGEEKGARDDEERVLDGLLMQYS
Sbjct: 301 NVKPQPARDINIDDVGSSTIDVTTKISSSRNKGKETMIGEEKGARDDEERVLDGLLMQYS 360

Query: 361 TKKTNQESKSRGKSNLKPQRQQEKDNIEHQRPTSRAVSLPPDQNEPMKKHTRTNSFVHPK 420
            KKTNQESKSRGKSNLKPQRQQEKDNIEHQRPT+RAVSLP DQNE  KKHTRTNSFVHPK
Sbjct: 361 KKKTNQESKSRGKSNLKPQRQQEKDNIEHQRPTTRAVSLPTDQNESTKKHTRTNSFVHPK 420

Query: 421 LPEYDQLAARIAALKEK 435
           LPEYDQLAARIAALKEK
Sbjct: 421 LPEYDQLAARIAALKEK 436

BLAST of Chy1G020650 vs. NCBI nr
Match: XP_008462153.1 (PREDICTED: uncharacterized protein LOC103500577 [Cucumis melo])

HSP 1 Score: 779 bits (2012), Expect = 2.33e-282
Identity = 414/437 (94.74%), Postives = 420/437 (96.11%), Query Frame = 0

Query: 1   MTKTRLDTTRKKKNAVLKYLKNDIVELLKSRLDYNAYNRAEGFLVERNVLRCYELIDEFC 60
           MTKTRLDT RKKKNAVLKYLKNDIVELLKSRLDYNA+NRAEGFLVERNVLRCYELIDEFC
Sbjct: 20  MTKTRLDTIRKKKNAVLKYLKNDIVELLKSRLDYNAFNRAEGFLVERNVLRCYELIDEFC 79

Query: 61  GTISNQISVLNKESECPDECKEAVATLIYAAARFADLPELRELRNLFTEKYGSSFGSFTN 120
           GTISNQI VLNKESECPDECKEAVATLIYAAARFADLPELRELRNLFTEKYGSSFGSFTN
Sbjct: 80  GTISNQIPVLNKESECPDECKEAVATLIYAAARFADLPELRELRNLFTEKYGSSFGSFTN 139

Query: 121 KEFIENSRTTTQTKEMKIQLLQEIAQETAIDWNSKALEQQLYTPPPENELDGERSGATKR 180
           KEFIE SR TTQTKEMKIQLLQEIAQETAIDWNSKALEQQLYTPPP+NELDGERSGATKR
Sbjct: 140 KEFIEKSRATTQTKEMKIQLLQEIAQETAIDWNSKALEQQLYTPPPQNELDGERSGATKR 199

Query: 181 NKTKVVSVPVYEKKANSPRNKNNSDNESIFDSRSEGNTTETSTGDSTDQDVHKGVLGDEV 240
           NKTKVVSVPVYE+KANSPRNKNNSDNESIFDSRSEGNTTETSTGDSTDQD+HKGV  DEV
Sbjct: 200 NKTKVVSVPVYERKANSPRNKNNSDNESIFDSRSEGNTTETSTGDSTDQDIHKGVSEDEV 259

Query: 241 -DQKPFNRRFVQPPYLKTKPIKTEANGEELPRKVTIENDESN-PKSPTEEKPKPRSVRRR 300
            DQKPFN RFVQPPYLKTKPIKTEAN +E PRKVTIENDESN PKSPTEEKPKPRSVRRR
Sbjct: 260 EDQKPFNHRFVQPPYLKTKPIKTEANEKE-PRKVTIENDESNNPKSPTEEKPKPRSVRRR 319

Query: 301 IVKPQPARDINIDDVGSSTIDVTKKISSSRNKGKETMIGEEKGARDDEERVLDGLLMQYS 360
            VKPQPARDINIDDVGSSTIDVT KISSSRNKGKETMIGEEKGARDDEERVLDGLLMQYS
Sbjct: 320 NVKPQPARDINIDDVGSSTIDVTTKISSSRNKGKETMIGEEKGARDDEERVLDGLLMQYS 379

Query: 361 TKKTNQESKSRGKSNLKPQRQQEKDNIEHQRPTSRAVSLPPDQNEPMKKHTRTNSFVHPK 420
            KKTNQESKSRGKSNLKPQRQQEKDNIEHQRPT+RAVSLP DQNE  KKHTRTNSFVHPK
Sbjct: 380 KKKTNQESKSRGKSNLKPQRQQEKDNIEHQRPTTRAVSLPTDQNESTKKHTRTNSFVHPK 439

Query: 421 LPEYDQLAARIAALKEK 435
           LPEYDQLAARIAALKEK
Sbjct: 440 LPEYDQLAARIAALKEK 455

BLAST of Chy1G020650 vs. NCBI nr
Match: XP_038897244.1 (uncharacterized protein LOC120085370 [Benincasa hispida])

HSP 1 Score: 678 bits (1749), Expect = 4.02e-242
Identity = 372/450 (82.67%), Postives = 396/450 (88.00%), Query Frame = 0

Query: 1   MTKTRLDTTRKKKNAVLKYLKNDIVELLKSRLDYNAYNRAEGFLVERNVLRCYELIDEFC 60
           MTKTRLD+ RKKK AVLKYLKNDIVELLKSRLDYNAYNRAEGFLVERNVLRCY LIDEFC
Sbjct: 20  MTKTRLDSIRKKKKAVLKYLKNDIVELLKSRLDYNAYNRAEGFLVERNVLRCYLLIDEFC 79

Query: 61  GTISNQISVLNKESECPDECKEAVATLIYAAARFADLPELRELRNLFTEKYGSSFGSFTN 120
           GTIS+QI VL+KESECP+ECKEAVATLIYAAARFADLPELRELR+LFTEKYGSSFGSFTN
Sbjct: 80  GTISSQIPVLSKESECPEECKEAVATLIYAAARFADLPELRELRSLFTEKYGSSFGSFTN 139

Query: 121 KEFIENSRTTTQTKEMKIQLLQEIAQETAIDWNSKALEQQLYTPPPENELDGERSGATKR 180
           KE IE SR T QTKEMKIQLLQEIAQ++AIDWNSKALEQQLYTPPP+NE  GERS   KR
Sbjct: 140 KELIEKSRATAQTKEMKIQLLQEIAQDSAIDWNSKALEQQLYTPPPQNEPAGERSATIKR 199

Query: 181 NKTKVVSVPVYEKKANSPRNKNNSDNESIFDSRSEGNTTETSTGDSTDQDVHKGVLGDEV 240
           NKTK+V+VPV+E+K  SPR K+NSDNESIFDSRSEGNTTETSTGDSTDQDVHKG+  DEV
Sbjct: 200 NKTKIVTVPVHERKTISPRKKDNSDNESIFDSRSEGNTTETSTGDSTDQDVHKGISEDEV 259

Query: 241 -DQKPFNRRFVQPPYLKTKPIKTEANGEELPRKVTI-ENDESN---PKSPTEEKPKPRSV 300
            DQKPF  RFV PPYLKTKPIKTEAN EE  +KVT+  NDESN   PKSPTEEKPKPRSV
Sbjct: 260 EDQKPFYLRFVPPPYLKTKPIKTEANVEES-QKVTVGNNDESNKQHPKSPTEEKPKPRSV 319

Query: 301 RRRIVKPQPARDINIDDVGSSTIDVTKKISSSRNKGKETMIGEEKGARDDEERVLDGLLM 360
           RRR VKPQPARDINIDD GSSTID  +K S S+NKGKETMIGEE+GA DDEERVLDGLLM
Sbjct: 320 RRRNVKPQPARDINIDDAGSSTIDGAEKTSLSQNKGKETMIGEERGAGDDEERVLDGLLM 379

Query: 361 QYSTKKTNQESKSRGKSNLKPQRQQEKDNIEHQRPTS----------RAVSLPPDQNEPM 420
           QYS KKT+QES +RGK NLKPQRQQEKDN E Q+P S          RAVSLP DQ+EP+
Sbjct: 380 QYSRKKTSQES-NRGKGNLKPQRQQEKDNAEPQKPKSTKTEDSFVPARAVSLPTDQSEPV 439

Query: 421 KKHTRTNSFVHPKLPEYDQLAARIAALKEK 435
           KKHTRTNSFVHPKLPEYDQLAARIAALKEK
Sbjct: 440 KKHTRTNSFVHPKLPEYDQLAARIAALKEK 467

BLAST of Chy1G020650 vs. NCBI nr
Match: KAG6575541.1 (IST1-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 566 bits (1460), Expect = 2.08e-198
Identity = 318/441 (72.11%), Postives = 353/441 (80.05%), Query Frame = 0

Query: 1   MTKTRLDTTRKKKNAVLKYLKNDIVELLKSRLDYNAYNRAEGFLVERNVLRCYELIDEFC 60
           MTKTRLDT RKKK  VLK+LKNDIVELLKSRLDYNAYNRAEGFLVE+NVLRCY+LIDEFC
Sbjct: 20  MTKTRLDTIRKKKKVVLKFLKNDIVELLKSRLDYNAYNRAEGFLVEQNVLRCYDLIDEFC 79

Query: 61  GTISNQISVLNKESECPDECKEAVATLIYAAARFADLPELRELRNLFTEKYGSSFGSFTN 120
           GTI   ISVLNKESECPDECKEAVA+LIYAAARF+DLPELR LR+LFT +YG+SFGSFTN
Sbjct: 80  GTIFKHISVLNKESECPDECKEAVASLIYAAARFSDLPELRVLRSLFTGRYGTSFGSFTN 139

Query: 121 KEFIENSRTTTQTKEMKIQLLQEIAQETAIDWNSKALEQQLYTPPPENELDGERSGATKR 180
           KE +E  R   QTKE K+QLLQEIAQE+AIDWNSKALEQQLY PP +NELD ERS +T R
Sbjct: 140 KELVEKLRAVAQTKETKLQLLQEIAQESAIDWNSKALEQQLYVPP-QNELDRERSTSTNR 199

Query: 181 NKTKVVSVPVYEKKANSPRNKNNSDNESIFDSRSEGNTTETSTGDS-TDQDVHKGVLG-D 240
           NK+K+VSVPVY +  NSPR K NSD++SIFDSRSEGNTTETS GDS TDQDVHKGV+  D
Sbjct: 200 NKSKIVSVPVYGRTTNSPRKKENSDDDSIFDSRSEGNTTETSMGDSSTDQDVHKGVVSED 259

Query: 241 EV-DQKPFNRRFVQPPYLKTKPIKTEANGEELPRKVTIENDESNPKSPTEEKPKPRSVRR 300
           EV DQKPF  RF+ PPYLKTKP K E N EE P+ VT EN  ++PK+  EEKPKPRSVRR
Sbjct: 260 EVEDQKPFYLRFITPPYLKTKPTKKEENVEE-PQNVTEEN-RNDPKTAIEEKPKPRSVRR 319

Query: 301 RIVKPQPARDINIDDVGSSTIDVTKKISSSRNKGKETMIGEEKGARDDEERVLDGLLMQY 360
           R  KPQPAR INIDD G S  D   KISS RNKGKE M GEEKG  DDEER+LDGLL  Y
Sbjct: 320 RHAKPQPARSINIDDAGGSAADGAGKISSGRNKGKEAMTGEEKGVEDDEERMLDGLLKLY 379

Query: 361 STKKTNQESKSRGKSNLKPQRQQEKDNIEHQRPTSRAVSLPPDQNEPM---KKHTRTNSF 420
           S KKT QE K     N +PQR +     +   PT +++SLPP + EP+   KKH R NS+
Sbjct: 380 SKKKTRQEDKD----NAEPQRPKNTRTEDAYVPT-KSLSLPPPRTEPIEPVKKHARANSY 439

Query: 421 VHPKLPEYDQLAARIAALKEK 435
           VHPKLP+YDQLAAR AALKEK
Sbjct: 440 VHPKLPDYDQLAARFAALKEK 452

BLAST of Chy1G020650 vs. TAIR 10
Match: AT1G79910.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 234.2 bits (596), Expect = 2.0e-61
Identity = 138/308 (44.81%), Postives = 198/308 (64.29%), Query Frame = 0

Query: 1   MTKTRLDTTRKKKNAVLKYLKNDIVELLKSRLDYNAYNRAEGFLVERNVLRCYELIDEFC 60
           +TKTR+DT ++KKN+V KYLKNDIV+LLK+ LDYNAY RAEG + E+  L CYE +++FC
Sbjct: 20  ITKTRVDTVKRKKNSVCKYLKNDIVDLLKNSLDYNAYGRAEGLIEEKRRLACYEFLEQFC 79

Query: 61  GTISNQISVLNKESECPDECKEAVATLIYAAARFADLPELRELRNLFTEKYGSSFGSFTN 120
             +++ +S+L K   CPDEC+EA+++L+YAAAR +++PELR+LR+LF E+YG++   F N
Sbjct: 80  NCVASNVSLLQKSIRCPDECREAISSLVYAAARVSEVPELRDLRSLFAERYGNTLDQFVN 139

Query: 121 KEFIENSRTTTQTKEMKIQLLQEIAQETAIDWNSKALEQQLYTPPPENELDGERSGATKR 180
            EF+E  +    +KEMK++LLQEIA+E +I W++K+LEQ+LYTPP  + +  E +   K 
Sbjct: 140 PEFVERFKAEPPSKEMKVELLQEIAREYSIKWDAKSLEQRLYTPPHHHHVHTE-TEEPKT 199

Query: 181 NKTKVVSVPVYEKKAN---SPRNKNNSDNESIFDSRSEGNTTETSTGDSTDQDVHKGVLG 240
            KT        EK+++     R  +  D+     S SE ++  TS    T  +       
Sbjct: 200 EKTNSEPEKTMEKRSSLSFHGREDSMDDSNKSIMSTSEEDSMSTSGSCMTGPE------- 259

Query: 241 DEVDQKPFNRRFVQP-PYLKTKPIKTEANGEELPRKVTIENDESNPKSPTEEKPKPRSVR 300
           D+ + KPF  RF+ P PY K K  K E+    LP K+T  +   + +SPT  KPKPRSVR
Sbjct: 260 DDPEMKPFYYRFMTPAPYTKPKIEKQES----LPEKMTTSH-LMDTESPTGGKPKPRSVR 314

Query: 301 RRIVKPQP 305
           RR   P P
Sbjct: 320 RRFANPPP 314

BLAST of Chy1G020650 vs. TAIR 10
Match: AT1G79910.2 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 177.2 bits (448), Expect = 2.9e-44
Identity = 109/268 (40.67%), Postives = 162/268 (60.45%), Query Frame = 0

Query: 41  EGFLVERNVLRCYELIDEFCGTISNQISVLNKESECPDECKEAVATLIYAAARFADLPEL 100
           EG + E+  L CYE +++FC  +++ +S+L K   CPDEC+EA+++L+YAAAR +++PEL
Sbjct: 46  EGLIEEKRRLACYEFLEQFCNCVASNVSLLQKSIRCPDECREAISSLVYAAARVSEVPEL 105

Query: 101 RELRNLFTEKYGSSFGSFTNKEFIENSRTTTQTKEMKIQLLQEIAQETAIDWNSKALEQQ 160
           R+LR+LF E+YG++   F N EF+E  +    +KEMK++LLQEIA+E +I W++K+LEQ+
Sbjct: 106 RDLRSLFAERYGNTLDQFVNPEFVERFKAEPPSKEMKVELLQEIAREYSIKWDAKSLEQR 165

Query: 161 LYTPPPENELDGERSGATKRNKTKVVSVPVYEKKAN---SPRNKNNSDNESIFDSRSEGN 220
           LYTPP  + +  E +   K  KT        EK+++     R  +  D+     S SE +
Sbjct: 166 LYTPPHHHHVHTE-TEEPKTEKTNSEPEKTMEKRSSLSFHGREDSMDDSNKSIMSTSEED 225

Query: 221 TTETSTGDSTDQDVHKGVLGDEVDQKPFNRRFVQP-PYLKTKPIKTEANGEELPRKVTIE 280
           +  TS    T  +       D+ + KPF  RF+ P PY K K  K E+    LP K+T  
Sbjct: 226 SMSTSGSCMTGPE-------DDPEMKPFYYRFMTPAPYTKPKIEKQES----LPEKMTTS 285

Query: 281 NDESNPKSPTEEKPKPRSVRRRIVKPQP 305
           +   + +SPT  KPKPRSVRRR   P P
Sbjct: 286 H-LMDTESPTGGKPKPRSVRRRFANPPP 300

BLAST of Chy1G020650 vs. TAIR 10
Match: AT4G32350.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 136.7 bits (343), Expect = 4.4e-32
Identity = 93/306 (30.39%), Postives = 156/306 (50.98%), Query Frame = 0

Query: 1   MTKTRLDTTRKKKNAVLKYLKNDIVELLKSRLDYNAYNRAEGFLVERNVLRCYELIDEFC 60
           +TK R+D  R+K+NA +K+LK D+ +L+ +  DYNA++RA G L E   L   + +++ C
Sbjct: 20  LTKNRIDVLRRKRNATIKFLKRDLADLIINGHDYNAFSRAGGLLDELRYLWSLDFVEQTC 79

Query: 61  GTISNQISVLNKESECPDECKEAVATLIYAAARFADLPELRELRNLFTEKYGSSFGSFTN 120
             +  Q+S + K  ECP++C+EA+++L++AA+ F++LPELRELR +F EKY  S   F N
Sbjct: 80  DFVYKQLSTMQKTPECPEDCREAISSLMFAASGFSELPELRELRQMFHEKYTDSLALFVN 139

Query: 121 KEFIENSRTTTQTKEMKIQLLQEIAQETAIDWNSKALEQQL------------------Y 180
           +E +EN  +   + E K++L++++A E +I W+SK  E+++                  Y
Sbjct: 140 QELVENMSSKPFSMEKKVKLMEDVALEFSIRWDSKDFEKRIVRQNSISVMETPKSTNDKY 199

Query: 181 TP-------PPENELDGERSGATKRNKTKVVSV---PVYEKKANSPRNKNNSDNESIFDS 240
            P       P   E +G  +G +   KT   S    P+++    S +N    +   +   
Sbjct: 200 KPVDRNMALPKREEFEGSENGVSLNRKTAEASERRDPLFQSDKESYQNGLRGNQRGLTYK 259

Query: 241 RSEGNTTETSTGDSTDQDVHKGVLGDEVDQKPFNRRFVQ-PPYLKTKPIKTEANGEELPR 278
               N    S  +S D         ++ ++K F     Q P   K +PI  E  G+ +  
Sbjct: 260 ERSENVLHASRSESKD---------NKAERKEFYLHSKQNPAREKHQPIFNE--GDTIVM 314

BLAST of Chy1G020650 vs. TAIR 10
Match: AT1G52315.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 127.9 bits (320), Expect = 2.0e-29
Identity = 82/233 (35.19%), Postives = 134/233 (57.51%), Query Frame = 0

Query: 3   KTRLDTTRKKKNAVLKYLKNDIVELLKSRLDYNAYNRAEGFLVERNVLRCYELIDEFCGT 62
           K R+D  R+K+ A+++  K DIV  LK+  D  AY RAE  L E  ++ CY+LI+ FC  
Sbjct: 22  KIRIDIVRRKRIAMVRNYKTDIVNFLKNGQDSEAYRRAELLLEELRIISCYDLIERFCDC 81

Query: 63  ISNQISVLNKESECPDECKEAVATLIYAAARFADLPELRELRNLFTEKYGSSFGSFTNKE 122
           IS  +S++ K+ ECP+EC+EAV++LIYA A   D+PEL++LR +FT+++G+   S  N E
Sbjct: 82  ISENLSLMLKKRECPEECREAVSSLIYATAWVPDVPELKDLRAVFTKRFGNFIASSVNHE 141

Query: 123 FIENSR-TTTQTKEMKIQLLQEIAQETAIDWNSKALEQQLYTPPPENELDGERSGATKRN 182
            +E +      ++E+KIQ ++++A E +I+W+   L+  L            R  +  + 
Sbjct: 142 LVEKTELLRPPSRELKIQTVKDVANEFSINWDPTPLKLMLL-----------RETSALQI 201

Query: 183 KTKVVSVPVY-EKKANSPRNKNNSDNESI------FDSRSEGNTTETSTGDST 228
           K KV +   Y  K         +SD+ES+       DS S+G+ + +S+  S+
Sbjct: 202 KDKVETRADYLNKDTEKSFMDEHSDDESVLSESWRIDSLSKGSLSSSSSSSSS 243

BLAST of Chy1G020650 vs. TAIR 10
Match: AT4G35730.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 100.1 bits (248), Expect = 4.5e-21
Identity = 111/453 (24.50%), Postives = 200/453 (44.15%), Query Frame = 0

Query: 1   MTKTRLDTTRKKKNAVLKYLKNDIVELLKSRLDYNAYNRAEGFLVERNVLRCYELIDEFC 60
           M   R+   R K+  V+K ++ DI  LL+S  D  A  R E  + E+N+    E+I+ FC
Sbjct: 37  MAVARIKLIRNKRLVVVKQMRRDIAVLLQSGQDATARIRVEHVIREQNIQAANEIIELFC 96

Query: 61  GTISNQISVLNKESECPDECKEAVATLIYAAARFADLPELRELRNLFTEKYGSSFGSF-- 120
             I ++++++ K+ +CP + KE +A+LI+AA R +++PEL +LR++F +KYG  F S   
Sbjct: 97  ELIVSRLTIITKQKQCPVDLKEGIASLIFAAPRCSEIPELGDLRDIFAKKYGKDFVSAAT 156

Query: 121 -------TNKEFIENSRTTTQTKEMKIQLLQEIAQETAIDWNSKALEQQLYTPPPENELD 180
                   N+  I+         E K+++++EIA+E  +DW++   EQ+L   P E  +D
Sbjct: 157 DLRPSCGVNRMLIDKLSVRNPGGEYKLKIMKEIAKEFQVDWDTTETEQEL-LKPQEESID 216

Query: 181 GER---SGATKRNKTKVVSVPVYEKKANSPRNKNNSDNESIFDSRSEGNTTETSTGDSTD 240
           G R   S ++       ++ P+   KA      + S N    D+ S           +  
Sbjct: 217 GPRKFVSASSLPVNRAAINEPIDPTKAVPRSTSSMSINTHYHDTESAAEAATELAKQAVA 276

Query: 241 QDVHKGVLGDEVD--QKPFNRRFVQPPYLKTKPIKTEANGEELPRKVTIENDESNPKSPT 300
                 +L    D   K F+       + K        +     R+ + +++ S+  +  
Sbjct: 277 AAQVASLLATRRDSSNKEFSVSSDHSTHQKDSQYMDHHHHHPGSRRQSRDSETSSYYA-- 336

Query: 301 EEKPKPRSVRRRIVKPQPARDINIDDVGSSTIDVTKKISSSRNKGKETMIGEEKGARDDE 360
               KP +  R +      R  + ++ G +  D  ++ +++  + KETM           
Sbjct: 337 ----KPGAENRGM-----GRRHSYNNPGINESDYEEEYTNTEAEAKETMRRRH------- 396

Query: 361 ERVLDGLLMQYSTKKTNQESKSRGKSNLKPQRQQEKDNIEHQRPTSRAVSLPPDQNEPM- 420
                     Y+ +     + S  K +     ++E +  E  +   R  SLPP++  P  
Sbjct: 397 ---------SYNPRSVPPPATSEIKFDESDYYEEETEPDEPSQ--GRVSSLPPNRAPPQA 456

Query: 421 -----KKHTRTNSFVHPKLPEYDQLAARIAALK 434
                 +   +   VHPKLP+YD LAAR  A++
Sbjct: 457 PQSGESRQDSSGHQVHPKLPDYDILAARFEAIR 459

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q54I397.5e-1325.58IST1-like protein OS=Dictyostelium discoideum OX=44689 GN=DDB_G0289029 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0K9261.1e-23297.93Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G446760 PE=3 SV=1[more]
A0A5D3BX002.1e-22094.74Putative Regulator of Vps4 activity in the MVB pathway protein OS=Cucumis melo v... [more]
A0A1S3CGT52.1e-22094.74uncharacterized protein LOC103500577 OS=Cucumis melo OX=3656 GN=LOC103500577 PE=... [more]
A0A6J1GMM11.1e-15571.88uncharacterized protein LOC111455825 OS=Cucurbita moschata OX=3662 GN=LOC1114558... [more]
A0A6J1JV691.0e-15370.98uncharacterized protein LOC111488629 OS=Cucurbita maxima OX=3661 GN=LOC111488629... [more]
Match NameE-valueIdentityDescription
XP_004141848.11.59e-29897.93uncharacterized protein LOC101220202 [Cucumis sativus] >KGN45359.1 hypothetical ... [more]
KAA0059291.11.14e-28294.74putative Regulator of Vps4 activity in the MVB pathway protein [Cucumis melo var... [more]
XP_008462153.12.33e-28294.74PREDICTED: uncharacterized protein LOC103500577 [Cucumis melo][more]
XP_038897244.14.02e-24282.67uncharacterized protein LOC120085370 [Benincasa hispida][more]
KAG6575541.12.08e-19872.11IST1-like protein, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
AT1G79910.12.0e-6144.81Regulator of Vps4 activity in the MVB pathway protein [more]
AT1G79910.22.9e-4440.67Regulator of Vps4 activity in the MVB pathway protein [more]
AT4G32350.14.4e-3230.39Regulator of Vps4 activity in the MVB pathway protein [more]
AT1G52315.12.0e-2935.19Regulator of Vps4 activity in the MVB pathway protein [more]
AT4G35730.14.5e-2124.50Regulator of Vps4 activity in the MVB pathway protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR042277Vacuolar protein sorting-associated protein IST1-likeGENE3D1.20.1260.60coord: 1..158
e-value: 5.0E-44
score: 152.1
IPR005061Vacuolar protein sorting-associated protein Ist1PFAMPF03398Ist1coord: 1..150
e-value: 5.8E-41
score: 140.1
IPR005061Vacuolar protein sorting-associated protein Ist1PANTHERPTHR12161IST1 FAMILY MEMBERcoord: 1..435
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 162..419
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 364..389
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 231..245
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 261..305
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 325..351
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 201..229
NoneNo IPR availablePANTHERPTHR12161:SF60REGULATOR OF VPS4 ACTIVITY IN THE MVB PATHWAY PROTEINcoord: 1..435

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy1G020650.1Chy1G020650.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015031 protein transport