Chy1G020640 (gene) Cucumber (hystrix) v1

Overview
NameChy1G020640
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
Descriptionnitrate regulatory gene2 protein-like
LocationchrH01: 25468350 .. 25470727 (-)
RNA-Seq ExpressionChy1G020640
SyntenyChy1G020640
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTTGTTCTCAATCCAAGATCGAAAATGAAGAAGTGGTTTCTCGGTGTAAGGATCGTAAGATGTTCATGAAAGATGCTGTCACCGCCAGGAATGCATTCGCTGCGGCTCATTCTTCTTATGCCATGTCCTTGAAGAATACCGGCGCTGTTTTGAGTGATTACGCTCATGGTGAAGGCCCGCCGGCTCCCTCGTCTCTCCCTGGCTCCGCTGTTGTTCAATCGGCTGCTGCCGCGGGTTATAATAGCTTGCCTCCACCGCCGCCTCCACTTCCTGGTTCCCCTGGTATGCCGCTTGAAATCAAGGCGTCGAAGGTTGAACCGAAGCGTGTGGAGCCGGCTATTCAGGAGGTGGATGAGAATGATTTTGAGATTGAATGTTCCGTTGGTCCATTGCGGAGGAGGAGAAGCAATAGAGACGGTAGTGGACGTGGCGGTAGAGCTGGTCCTGGGGAGCTTGCGGAAGAAGAAAACGGTCCGCCTCCGCCCTTTCCGCCTTCGAGCGAAAACCGTCGTGTTCCCGTACCGTCGCCTCAGGATTCGACCTACGATTATCTGTTCTCGGTTGATAATATGCCAGCTCCGACGTTGAGCGGGGTCGAGGATTTTGGCGCTAATACGGAAACAGTAGAACGGAGAGCGGCGATGGAGAAATCCGGGGAAGAACCGCCGTCGTCTTCGGCGGGGAAAACGTCGAAGAAGATGAAGCAAGTAGGTTATCCAGGTTCGAGCGAGGGTAAAAGGATCGTTCAAGGAAATATTAATCTGCTACAGATCTTCATGGACCTTGATGATCATTTTCTTAAAGCCTCTGAAAGTGCTCACGATGTTTCGAAGATGCTGGAAGCAACTCGACTTCATTTCCACTCAAATTTTGCCGATAATCGAGGTATTTCTCTCGATTTCCATGTCAAATTCATATTTAAACATTCAATCATTTGAAGGAACAGAGGAACTTCAATTTATGAAATTTGATCTCTCAGGACATATCGATCATTCTGCTAGAGTGATGCGTGTTATAACATGGAACAGATCATTTAGAGGGTTACCCAACAACGATGATTTGAATGATGGTTTTGATACAGAGGAGAACGAAACTCATGCCACTGTATTGGATAAATTGCTCGCTTGGGAGAAGAAACTATTTGAGGAAGTGAAGGTTAAAAAATTTTGAAACCTTCAATGTCTCTTGTTCTTGTATTGCACTTAAAAAATCCCTTGTTCTGACTGTTACTTAGATAGGCAGGTGAAATCATGAAGTTTGAGTATCAAAAGAAGGTTGCTGCACTCAACAAACTTAAGAAAAAGGGCTCCAATTTTGAAGCAATCGAGAAAGCTAAGGCCACTGTCAGCCATTTGCACACCAGATACATTGTGGACATGCAATCGATGGATTCCACTGTCTCTGAAATCAACCGCATTCGTGATGAACAATTGTATCCTAAACTCGTTCACCTTATCATTGGGTAAAACCACGATGAATTCATTAGTTTTGATTCTGTTTGATAATTATTTTGTTTTAGGTAATGTGTCAGACTAAATCAAGTTGTGGGTTGAATTGTAGAATGGCTAGTATGTGGGAAACCATGCATTTTCACCATGGAAGCCAATTGAAGGTTGTGGCTGCTCTTAGAATGCTGGATATCTCTCAATCCCCAAAGGAAACAAGTGATCACCACCATGAACGAACAGTGCAGCTTTGGGCTGTGGTGCAAGAGTGGCACTCTCAATTGGAGAAGCTTGTAAACCGTCAAAAAGATTACATCAAAGCACTCTCAAATTGGTTGAGATTGAATCTGATCCCTACCGAAAGCAGCTTGAAGGAGAAGGTTTCTTCTCCTCCAAGGGTTCGCAGCCCTCCAATTCAAATCCTCCTTCACGCTTGGCAAGACCATCTCGAGAAGCTCCCTGACGAGGTCCTAAGAAACGCCATATTCACTTTTGCAACTGTGATTCACACCATTATGCAAAGCCAGGAGGAAGAGATGAAGTTGAAGCTAAAATGTCAGGAGACCGAGAAGGAGCTTGCCCGGAAAAGTAAGCAATTCAAGGACTGGCAGAAGAAGTATGTGCAACGGAGAGGTTCGAATGCCGATGAAGTGGACATGGAAGAACCTGCCGACAAAGACGCCATTGCAGAGCGGCAAGCTGCAGTGGAGGCCGTGGAGAAGAAGCTGGAGGAGGAAAGGGAAGAACACCAAAAACTATGCCTCCATGTAAGGGAAAAGTCTTTGGGGAGCCTAAAAAATCAGTTGCCAGAGCTTTTCAGGGCATTGTTCGAGTTCTCTCTTGCTTGTTCACGCATGTATAGGCACTTGAAATCAATATCACAGCCAATGCCCAATGGGCCACAAAATCAAACAACAACACTAGGATGA

mRNA sequence

ATGGGTTGTTCTCAATCCAAGATCGAAAATGAAGAAGTGGTTTCTCGGTGTAAGGATCGTAAGATGTTCATGAAAGATGCTGTCACCGCCAGGAATGCATTCGCTGCGGCTCATTCTTCTTATGCCATGTCCTTGAAGAATACCGGCGCTGTTTTGAGTGATTACGCTCATGGTGAAGGCCCGCCGGCTCCCTCGTCTCTCCCTGGCTCCGCTGTTGTTCAATCGGCTGCTGCCGCGGGTTATAATAGCTTGCCTCCACCGCCGCCTCCACTTCCTGGTTCCCCTGGTATGCCGCTTGAAATCAAGGCGTCGAAGGTTGAACCGAAGCGTGTGGAGCCGGCTATTCAGGAGGTGGATGAGAATGATTTTGAGATTGAATGTTCCGTTGGTCCATTGCGGAGGAGGAGAAGCAATAGAGACGGTAGTGGACGTGGCGGTAGAGCTGGTCCTGGGGAGCTTGCGGAAGAAGAAAACGGTCCGCCTCCGCCCTTTCCGCCTTCGAGCGAAAACCGTCGTGTTCCCGTACCGTCGCCTCAGGATTCGACCTACGATTATCTGTTCTCGGTTGATAATATGCCAGCTCCGACGTTGAGCGGGGTCGAGGATTTTGGCGCTAATACGGAAACAGTAGAACGGAGAGCGGCGATGGAGAAATCCGGGGAAGAACCGCCGTCGTCTTCGGCGGGGAAAACGTCGAAGAAGATGAAGCAAGTAGGTTATCCAGGTTCGAGCGAGGGTAAAAGGATCGTTCAAGGAAATATTAATCTGCTACAGATCTTCATGGACCTTGATGATCATTTTCTTAAAGCCTCTGAAAGTGCTCACGATGTTTCGAAGATGCTGGAAGCAACTCGACTTCATTTCCACTCAAATTTTGCCGATAATCGAGGACATATCGATCATTCTGCTAGAGTGATGCGTGTTATAACATGGAACAGATCATTTAGAGGGTTACCCAACAACGATGATTTGAATGATGGTTTTGATACAGAGGAGAACGAAACTCATGCCACTGTATTGGATAAATTGCTCGCTTGGGAGAAGAAACTATTTGAGGAAGTGAAGGCAGGTGAAATCATGAAGTTTGAGTATCAAAAGAAGGTTGCTGCACTCAACAAACTTAAGAAAAAGGGCTCCAATTTTGAAGCAATCGAGAAAGCTAAGGCCACTGTCAGCCATTTGCACACCAGATACATTGTGGACATGCAATCGATGGATTCCACTGTCTCTGAAATCAACCGCATTCGTGATGAACAATTGTATCCTAAACTCGTTCACCTTATCATTGGAATGGCTAGTATGTGGGAAACCATGCATTTTCACCATGGAAGCCAATTGAAGGTTGTGGCTGCTCTTAGAATGCTGGATATCTCTCAATCCCCAAAGGAAACAAGTGATCACCACCATGAACGAACAGTGCAGCTTTGGGCTGTGGTGCAAGAGTGGCACTCTCAATTGGAGAAGCTTGTAAACCGTCAAAAAGATTACATCAAAGCACTCTCAAATTGGTTGAGATTGAATCTGATCCCTACCGAAAGCAGCTTGAAGGAGAAGGTTTCTTCTCCTCCAAGGGTTCGCAGCCCTCCAATTCAAATCCTCCTTCACGCTTGGCAAGACCATCTCGAGAAGCTCCCTGACGAGGTCCTAAGAAACGCCATATTCACTTTTGCAACTGTGATTCACACCATTATGCAAAGCCAGGAGGAAGAGATGAAGTTGAAGCTAAAATGTCAGGAGACCGAGAAGGAGCTTGCCCGGAAAAGTAAGCAATTCAAGGACTGGCAGAAGAAGTATGTGCAACGGAGAGGTTCGAATGCCGATGAAGTGGACATGGAAGAACCTGCCGACAAAGACGCCATTGCAGAGCGGCAAGCTGCAGTGGAGGCCGTGGAGAAGAAGCTGGAGGAGGAAAGGGAAGAACACCAAAAACTATGCCTCCATGTAAGGGAAAAGTCTTTGGGGAGCCTAAAAAATCAGTTGCCAGAGCTTTTCAGGGCATTGTTCGAGTTCTCTCTTGCTTGTTCACGCATGTATAGGCACTTGAAATCAATATCACAGCCAATGCCCAATGGGCCACAAAATCAAACAACAACACTAGGATGA

Coding sequence (CDS)

ATGGGTTGTTCTCAATCCAAGATCGAAAATGAAGAAGTGGTTTCTCGGTGTAAGGATCGTAAGATGTTCATGAAAGATGCTGTCACCGCCAGGAATGCATTCGCTGCGGCTCATTCTTCTTATGCCATGTCCTTGAAGAATACCGGCGCTGTTTTGAGTGATTACGCTCATGGTGAAGGCCCGCCGGCTCCCTCGTCTCTCCCTGGCTCCGCTGTTGTTCAATCGGCTGCTGCCGCGGGTTATAATAGCTTGCCTCCACCGCCGCCTCCACTTCCTGGTTCCCCTGGTATGCCGCTTGAAATCAAGGCGTCGAAGGTTGAACCGAAGCGTGTGGAGCCGGCTATTCAGGAGGTGGATGAGAATGATTTTGAGATTGAATGTTCCGTTGGTCCATTGCGGAGGAGGAGAAGCAATAGAGACGGTAGTGGACGTGGCGGTAGAGCTGGTCCTGGGGAGCTTGCGGAAGAAGAAAACGGTCCGCCTCCGCCCTTTCCGCCTTCGAGCGAAAACCGTCGTGTTCCCGTACCGTCGCCTCAGGATTCGACCTACGATTATCTGTTCTCGGTTGATAATATGCCAGCTCCGACGTTGAGCGGGGTCGAGGATTTTGGCGCTAATACGGAAACAGTAGAACGGAGAGCGGCGATGGAGAAATCCGGGGAAGAACCGCCGTCGTCTTCGGCGGGGAAAACGTCGAAGAAGATGAAGCAAGTAGGTTATCCAGGTTCGAGCGAGGGTAAAAGGATCGTTCAAGGAAATATTAATCTGCTACAGATCTTCATGGACCTTGATGATCATTTTCTTAAAGCCTCTGAAAGTGCTCACGATGTTTCGAAGATGCTGGAAGCAACTCGACTTCATTTCCACTCAAATTTTGCCGATAATCGAGGACATATCGATCATTCTGCTAGAGTGATGCGTGTTATAACATGGAACAGATCATTTAGAGGGTTACCCAACAACGATGATTTGAATGATGGTTTTGATACAGAGGAGAACGAAACTCATGCCACTGTATTGGATAAATTGCTCGCTTGGGAGAAGAAACTATTTGAGGAAGTGAAGGCAGGTGAAATCATGAAGTTTGAGTATCAAAAGAAGGTTGCTGCACTCAACAAACTTAAGAAAAAGGGCTCCAATTTTGAAGCAATCGAGAAAGCTAAGGCCACTGTCAGCCATTTGCACACCAGATACATTGTGGACATGCAATCGATGGATTCCACTGTCTCTGAAATCAACCGCATTCGTGATGAACAATTGTATCCTAAACTCGTTCACCTTATCATTGGAATGGCTAGTATGTGGGAAACCATGCATTTTCACCATGGAAGCCAATTGAAGGTTGTGGCTGCTCTTAGAATGCTGGATATCTCTCAATCCCCAAAGGAAACAAGTGATCACCACCATGAACGAACAGTGCAGCTTTGGGCTGTGGTGCAAGAGTGGCACTCTCAATTGGAGAAGCTTGTAAACCGTCAAAAAGATTACATCAAAGCACTCTCAAATTGGTTGAGATTGAATCTGATCCCTACCGAAAGCAGCTTGAAGGAGAAGGTTTCTTCTCCTCCAAGGGTTCGCAGCCCTCCAATTCAAATCCTCCTTCACGCTTGGCAAGACCATCTCGAGAAGCTCCCTGACGAGGTCCTAAGAAACGCCATATTCACTTTTGCAACTGTGATTCACACCATTATGCAAAGCCAGGAGGAAGAGATGAAGTTGAAGCTAAAATGTCAGGAGACCGAGAAGGAGCTTGCCCGGAAAAGTAAGCAATTCAAGGACTGGCAGAAGAAGTATGTGCAACGGAGAGGTTCGAATGCCGATGAAGTGGACATGGAAGAACCTGCCGACAAAGACGCCATTGCAGAGCGGCAAGCTGCAGTGGAGGCCGTGGAGAAGAAGCTGGAGGAGGAAAGGGAAGAACACCAAAAACTATGCCTCCATGTAAGGGAAAAGTCTTTGGGGAGCCTAAAAAATCAGTTGCCAGAGCTTTTCAGGGCATTGTTCGAGTTCTCTCTTGCTTGTTCACGCATGTATAGGCACTTGAAATCAATATCACAGCCAATGCCCAATGGGCCACAAAATCAAACAACAACACTAGGATGA

Protein sequence

MGCSQSKIENEEVVSRCKDRKMFMKDAVTARNAFAAAHSSYAMSLKNTGAVLSDYAHGEGPPAPSSLPGSAVVQSAAAAGYNSLPPPPPPLPGSPGMPLEIKASKVEPKRVEPAIQEVDENDFEIECSVGPLRRRRSNRDGSGRGGRAGPGELAEEENGPPPPFPPSSENRRVPVPSPQDSTYDYLFSVDNMPAPTLSGVEDFGANTETVERRAAMEKSGEEPPSSSAGKTSKKMKQVGYPGSSEGKRIVQGNINLLQIFMDLDDHFLKASESAHDVSKMLEATRLHFHSNFADNRGHIDHSARVMRVITWNRSFRGLPNNDDLNDGFDTEENETHATVLDKLLAWEKKLFEEVKAGEIMKFEYQKKVAALNKLKKKGSNFEAIEKAKATVSHLHTRYIVDMQSMDSTVSEINRIRDEQLYPKLVHLIIGMASMWETMHFHHGSQLKVVAALRMLDISQSPKETSDHHHERTVQLWAVVQEWHSQLEKLVNRQKDYIKALSNWLRLNLIPTESSLKEKVSSPPRVRSPPIQILLHAWQDHLEKLPDEVLRNAIFTFATVIHTIMQSQEEEMKLKLKCQETEKELARKSKQFKDWQKKYVQRRGSNADEVDMEEPADKDAIAERQAAVEAVEKKLEEEREEHQKLCLHVREKSLGSLKNQLPELFRALFEFSLACSRMYRHLKSISQPMPNGPQNQTTTLG*
Homology
BLAST of Chy1G020640 vs. ExPASy Swiss-Prot
Match: Q9AQW1 (Protein ROLLING AND ERECT LEAF 2 OS=Oryza sativa subsp. japonica OX=39947 GN=REL2 PE=2 SV=1)

HSP 1 Score: 244.6 bits (623), Expect = 3.4e-63
Identity = 219/762 (28.74%), Postives = 356/762 (46.72%), Query Frame = 0

Query: 1   MGCSQSKIENEEVVSRCKDRKMFMKDAVTARNAFAAAHSSYAMSLKNTGAVLSDYAHGE- 60
           MGC+ SK+E E+ V RCK+R+  MK+AV +R   A+AH+ Y  SL+ T A LS +A G  
Sbjct: 1   MGCTASKVEQEDTVRRCKERRRHMKEAVASRQQLASAHADYLRSLRLTAAALSRFAQGHP 60

Query: 61  ------------------------GPPAPSSLPGSAV-VQSAAAAGYNSLPPPPPPLPG- 120
                                    PP PSS   S++   +     +   PPPPPP    
Sbjct: 61  SLAVSHHTAPVLLTTAAPALAPTPTPPPPSSTASSSLPPPTPLLPKHQQAPPPPPPTQSH 120

Query: 121 SPGMPLEIKASKVEPKRVE-PAIQEVDENDFEIECSVGPLRRRRSNRDGSGRGGRAGPGE 180
            P  P+ ++A +  P+R++ P I          + SV    R    +   G    +   +
Sbjct: 121 QPPPPVAVRAPRGGPRRLKVPHILS--------DSSVASPARSSFRKPVVGTPSSSSAWD 180

Query: 181 LAEEENGPPPPFPPS----------SENRRVPVPSPQDSTYDYLF--------SVDN--- 240
               EN  PP  P S           E  R+     ++    YL          VD+   
Sbjct: 181 W---ENFYPPSPPDSEFFDRRKADLEEANRLRELEEEEKARGYLHPHHLKEEDEVDDDDD 240

Query: 241 -----MPAPTLSGVEDFGANTETVERR--------------AAMEKSGEEPPSSSAG--- 300
                M        +D  A+T T E R              AA  + G   PS  A    
Sbjct: 241 EREEEMHCGGWEDDDDHYASTTTSETRSEEGEMGNRSECGFAARSEYGGTAPSEYAAAPL 300

Query: 301 ----KTSKKMKQVGYPGS----SEGKRIVQGNINLLQIFMDLDDHFLKASESAHDVSKML 360
               +   +  + G   S    +   R+V  +  L +I   ++++F+KA+E+ + VS++L
Sbjct: 301 PLPLRRRDERSEAGDSSSTVTAAAEMRMVIRHRTLAEIVAAIEEYFVKAAEAGNGVSELL 360

Query: 361 EATRLHFHSNFADNRGHIDHSARVMRVI--TW-NRSFRGLPNNDDLND-GFDTEENETHA 420
           EA+R     NF   +  + HS  ++  +  TW ++    +    D N    ++ E ++H 
Sbjct: 361 EASRAQLDRNFRQLKKTVYHSNSLLSSLSSTWTSKPPLAVRYKLDTNALEMESMEGKSHG 420

Query: 421 TVLDKLLAWEKKLFEEVKAGEIMKFEYQKKVAALNKLKKKGSNFEAIEKAKATVSHLHTR 480
           + L++LLAWEKKL++EVKA E +K E++KK++ L  L+ +G +   ++K KA+++ L + 
Sbjct: 421 STLERLLAWEKKLYQEVKARESVKIEHEKKLSTLQSLEYRGRDSTKLDKTKASINKLQSL 480

Query: 481 YIVDMQSMDSTVSEINRIRDEQLYPKLVHLIIGMASMWETMHFHHGSQLKVVAALRMLDI 540
            IV  Q+  +T S I R+RD +L P+LV L   + SMW +M+  H  Q ++V  +R L  
Sbjct: 481 IIVTSQAATTTSSAIVRVRDNELAPQLVELCFALLSMWRSMNHFHEIQNEIVQQVRGLVD 540

Query: 541 SQSPKETSDHHHERTVQLWAVVQEWHSQLEKLVNRQKDYIKALSNWLRLNLIPTESSLKE 600
           +   + TSD H   T  L A V  WHS   +L+  Q+DYI+AL  WL+L L   +S++ +
Sbjct: 541 NSMAESTSDLHRLATRDLEAAVSAWHSNFNRLIKYQRDYIRALYGWLKLTLFQVDSNIPQ 600

Query: 601 KVSSPPRVRSPPIQILLHAWQDHLEKLPDEVLRNAIFTFATVIHTIMQSQEEEMKLKLKC 660
           +  +   + S  +      W+  L++LPD     AI +F  V+H I   Q EEMK+K + 
Sbjct: 601 EAYT--SLISRELTTFCDEWKQALDRLPDASASEAIKSFVNVVHVIYTKQAEEMKIKKRT 660

Query: 661 QETEKELARKSKQFKDWQKKYVQR--------RGSNADEVDMEEPADKDAIAERQAAVEA 672
           +   KEL +K+   +  +KKY Q          GS  D ++      +D +AE++  +  
Sbjct: 661 ETYSKELEKKTNSLRAIEKKYYQSYSMVGLGLPGSGRDGIESHSFDARDPLAEKKTEIAQ 720

BLAST of Chy1G020640 vs. ExPASy Swiss-Prot
Match: A0A178VBJ0 (Protein ALTERED PHOSPHATE STARVATION RESPONSE 1 OS=Arabidopsis thaliana OX=3702 GN=APSR1 PE=2 SV=1)

HSP 1 Score: 239.6 bits (610), Expect = 1.1e-61
Identity = 203/717 (28.31%), Postives = 321/717 (44.77%), Query Frame = 0

Query: 1   MGCSQSKIENEEVVSRCKDRKMFMKDAVTARNAFAAAHSSYAMSLKNTGAVLSDYAHGE- 60
           MGC QS+I+++E+VSRCK RK ++K  V AR   + +H+ Y  SL+  G+ L  ++  E 
Sbjct: 1   MGCCQSRIDSKEIVSRCKARKRYLKHLVKARQTLSVSHALYLRSLRAVGSSLVHFSSKET 60

Query: 61  ------GPPAPSSLPGSAVVQSAAAAGYNSLPPPPPPLPGSPGMPLEIKASKVEPKRVEP 120
                  PP+PS  P                PP PPP P SPG       +      + P
Sbjct: 61  PLHLHHNPPSPSPPPPP--------------PPRPPPPPLSPGSETTTWTTTTTSSVLPP 120

Query: 121 AIQEVDENDFEIECSVGPLRRRRSNRDGSGRGGRAGPGELAEEENGPPPPFPPSSENRRV 180
                                                         PPPP PP       
Sbjct: 121 ----------------------------------------------PPPPPPP------- 180

Query: 181 PVPSPQDSTYDYLFSVDNMPAPTLSGVEDFGANTETVERRA----------AMEKSGEEP 240
             P P  ST+D  F    +P P  S  E++   T T  R A              +    
Sbjct: 181 --PPPPSSTWD--FWDPFIPPPPSSSEEEWEEETTTATRTATGTGSDAAVTTAPTTATPQ 240

Query: 241 PSSSAGKTSKKMKQVGYPGSSEGKRIVQGNINLLQIFMDLDDHFLKASESAHDVSKMLE- 300
            SS     SK        GS     + +   +L++I  ++D++FLKA++S   +S +LE 
Sbjct: 241 ASSVVSGFSKDTMTTTTTGSELAVVVSRNGKDLMEIIKEVDEYFLKAADSGAPLSSLLEI 300

Query: 301 ---ATRLHFHSNFADNRGHIDHSARVMRVITWNRSFRGLPNNDDLNDGFDTEEN---ETH 360
               T    HS         ++   +     W R F     ++  N G     N    +H
Sbjct: 301 STSITDFSGHSKSGKMYSSSNYECNLNPTSFWTRGFAPSKLSEYRNAGGVIGGNCIVGSH 360

Query: 361 ATVLDKLLAWEKKLFEEVKAGEIMKFEYQKKVAALNKLKKKGSNFEAIEKAKATVSHLHT 420
           ++ +D+L AWEKKL++EVK  E +K +++KKV  + +L+ K + +   EKAK  V  L +
Sbjct: 361 SSTVDRLYAWEKKLYQEVKYAESIKMDHEKKVEQVRRLEMKRAEYVKTEKAKKDVEKLES 420

Query: 421 RYIVDMQSMDSTVSEINRIRDEQLYPKLVHLIIGMASMWETMHFHHGSQLKVVAALRMLD 480
           +  V  Q++ S  +EI ++R+ +LYP+LV L+ G+  MW +M+  H  Q  +V  L+ L+
Sbjct: 421 QLSVSSQAIQSASNEIIKLRETELYPQLVELVKGLMCMWRSMYESHQVQTHIVQQLKYLN 480

Query: 481 ISQSPKETSDHHHERTVQLWAVVQEWHSQLEKLVNRQKDYIKALSNWLRLNLIPTESSLK 540
              S + TS+ H + T+QL   VQ+WH     LV  Q+DYI++L+ WLRL+L        
Sbjct: 481 TIPSTEPTSELHRQSTLQLELEVQQWHHSFCNLVKAQRDYIQSLTGWLRLSLF------- 540

Query: 541 EKVSSPPRVRS---PPIQILLHAWQDHLEKLPDEVLRNAIFTFATVIHTIMQSQEEEMKL 600
            + S  P VRS     I      W   ++++PD+V    I +F T +H I+  Q +E K 
Sbjct: 541 -QFSKNPLVRSSYESKIYSFCEEWHLAIDRIPDKVASEGIKSFLTAVHGIVAQQADEHKQ 600

Query: 601 KLKCQETEKELARKSKQFKDWQKKYVQRRGSNADEVDMEEPADKDAIAERQAAVEAVEKK 660
           K + +   K+  +KS   +  + KY            + E   K+ + E++  VE ++ K
Sbjct: 601 KKRTESMLKDFEKKSASLRALESKY--------SPYSVPESRKKNPVIEKRVKVEMLKGK 630

Query: 661 LEEEREEHQKLCLHVREKSLGSLKNQLPELFRALFEFSLACSR----MYRHLKSISQ 687
            EEE+ +H+K     R  +L +L+   P +F+A+  FS  C +    +Y   KSI +
Sbjct: 661 AEEEKSKHEKSVSVTRAMTLNNLQMGFPHVFQAMVGFSSVCMQAFESVYNQAKSIGE 630

BLAST of Chy1G020640 vs. ExPASy Swiss-Prot
Match: Q93YU8 (Nitrate regulatory gene2 protein OS=Arabidopsis thaliana OX=3702 GN=NRG2 PE=1 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 2.6e-47
Identity = 201/799 (25.16%), Postives = 348/799 (43.55%), Query Frame = 0

Query: 1   MGCSQSKIENEEVVSRCKDRKMFMKDAVTARNAFAAAHSSYAMSLKNTGAVLSDYAHGE- 60
           MGC+ SK++NE+ V RCKDR+  MK+AV AR+  AAAH+ Y  SL+ TG+ LS +A GE 
Sbjct: 1   MGCAASKLDNEDAVRRCKDRRRLMKEAVYARHHLAAAHADYCRSLRITGSALSSFASGEP 60

Query: 61  ---------------GPPAPSSLPGSAVVQ--SAAAAGYNSLPPPPPPLPGSPGMPLEIK 120
                           PP     P   V    S + A  +  PP   P   S   P  + 
Sbjct: 61  LSVSDQTPAVFLHTPPPPLSEQSPAKFVPPRFSPSPAPSSVYPPSTSPSVASSKQPSVMS 120

Query: 121 ASKVEPKRVEPAIQEVDENDFEIECSVGPLRRRRSN----------RDGSGRGGRAGPGE 180
            S    ++ +P    +     E   S  P R  RSN          ++ +     +    
Sbjct: 121 TSSNRRRKQQPK-PRLPHILSESSPSSSP-RSERSNFMPNLYPSAYQNSTYSATPSHASS 180

Query: 181 LAEEENGPPPPFPPS--------------------------------------------- 240
           +   EN  PP  P S                                             
Sbjct: 181 VWNWENFYPPSPPDSEFFNRKAQEKKHNSDNRFNDEDTETVRSEYDFFDTRKQKQKQFES 240

Query: 241 --------SENRRVPVPSPQDSTYDYLFSVDNMPA-------PTLSGVEDFGANTE--TV 300
                   +E  R  V   +   +D+  +  +  A            + + G  +E  + 
Sbjct: 241 MRNQVEEETETEREEVQCSEWEDHDHYSTTSSSDAAEEEEEDDDRESISEVGTRSEFGST 300

Query: 301 ERRAAMEKSGEEP---PSSSAGKTSKKMKQVGYPGSSEGK----------RIVQGNINLL 360
            R  +M +  ++P   P    G    K  +      S G           ++V  + +L 
Sbjct: 301 VRSNSMRRHHQQPSPMPQVYGGAEQSKYDKADDATISSGSYRGGGDIADMKMVVRHRDLK 360

Query: 361 QIFMDLDDHFLKASESAHDVSKMLEATRLHFHSNFADNRGHIDHSARVMRVI--TW-NRS 420
           +I   + ++F KA+ S   VS+MLE  R     +F+  +  + HS+ ++  +  TW ++ 
Sbjct: 361 EIIDAIKENFDKAAASGEQVSQMLELGRAELDRSFSQLKKTVIHSSSLLSNLSSTWTSKP 420

Query: 421 FRGLPNNDDLNDGFDTEENETHATVLDKLLAWEKKLFEEVKAGEIMKFEYQKKVAALNKL 480
              +    D         +++  + LD+LLAWEKKL+EE+KA E  K E++KK++ L   
Sbjct: 421 PLAVKYRIDTTALDQPNSSKSLCSTLDRLLAWEKKLYEEIKAREGFKIEHEKKLSQLQSQ 480

Query: 481 KKKGSNFEAIEKAKATVSHLHTRYIVDMQSMDSTVSEINRIRDEQLYPKLVHLIIGMASM 540
           + KG +   ++K KA+++ L +  IV  Q++ +T + I R+RD  L P+LV L  G   M
Sbjct: 481 EYKGEDEAKLDKTKASITRLQSLIIVTSQAVTTTSTAIIRLRDTDLVPQLVELCHGFMYM 540

Query: 541 WETMHFHHGSQLKVVAALR-MLDISQSPKETSDHHHERTVQLWAVVQEWHSQLEKLVNRQ 600
           W++MH +H +Q  +V  +R +++ S   + TS+ H + T  L + V  WHS    L+  Q
Sbjct: 541 WKSMHQYHETQNSIVEQVRGLINRSGKGESTSELHRQATRDLESAVSSWHSSFSSLIKFQ 600

Query: 601 KDYIKALSNWLRLNLIPTESSLKEKVSSPPRVRSPPIQILLHAWQDHLEKLPDEVLRNAI 660
           +D+I ++  W +L L+P    + ++ ++               W+  L+++PD V   AI
Sbjct: 601 RDFIHSVHAWFKLTLLP----VCQEDAANHHKEPLDAYAFCDEWKLALDRIPDTVASEAI 660

Query: 661 FTFATVIHTIMQSQEEEMKLKLKCQETEKELARKSKQFKDWQKKYVQRR-----GSNADE 679
            +F  V+H I   Q +E K+K + +   KEL +K+   ++ ++KY Q       G     
Sbjct: 661 KSFINVVHVISAKQADEHKIKKRTESASKELEKKASSVRNLERKYYQSYSMVGVGLPESG 720

BLAST of Chy1G020640 vs. ExPASy TrEMBL
Match: A0A0A0K7A3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G446740 PE=4 SV=1)

HSP 1 Score: 1345.1 bits (3480), Expect = 0.0e+00
Identity = 691/700 (98.71%), Postives = 695/700 (99.29%), Query Frame = 0

Query: 1   MGCSQSKIENEEVVSRCKDRKMFMKDAVTARNAFAAAHSSYAMSLKNTGAVLSDYAHGEG 60
           MGCSQSKIENEEVVSRCKDRKMFMKDAVTARNAFAAAHSSYAMSLKNTGAVLSDYAHGEG
Sbjct: 1   MGCSQSKIENEEVVSRCKDRKMFMKDAVTARNAFAAAHSSYAMSLKNTGAVLSDYAHGEG 60

Query: 61  PPAPSSLPGSAVVQSAAAAGYNSLPPPPPPLPGSPGMPLEIKASKVEPKRVEPAIQEVDE 120
           PPAPSSLPGS+VVQSAAAAGYNSLPPPPPPLPGSPGMPLEIKASKVEPKRVEP IQEVDE
Sbjct: 61  PPAPSSLPGSSVVQSAAAAGYNSLPPPPPPLPGSPGMPLEIKASKVEPKRVEPVIQEVDE 120

Query: 121 NDFEIECSVGPLRRRRSNRDGSGRGGRAGPGELAEEENGPPPPFPPSSENRRVPVPSPQD 180
           NDFEIECSVGPLRRRRSNRDGSGRGGRAGPGELAEEENGPPPPFPPSSENRRVPVPSPQD
Sbjct: 121 NDFEIECSVGPLRRRRSNRDGSGRGGRAGPGELAEEENGPPPPFPPSSENRRVPVPSPQD 180

Query: 181 STYDYLFSVDNMPAPTLSGVEDFGANTETVERRAAMEKSGEEPPSSSAGKTSKKMKQVGY 240
           STYDYLFSVDNMPAPTLSGVEDFGANTETVERRAA EKSGEEPPSSSAGKTSKKMKQVGY
Sbjct: 181 STYDYLFSVDNMPAPTLSGVEDFGANTETVERRAATEKSGEEPPSSSAGKTSKKMKQVGY 240

Query: 241 PGSSEGKRIVQGNINLLQIFMDLDDHFLKASESAHDVSKMLEATRLHFHSNFADNRGHID 300
           PGSSEGKRIV+G+INLLQIFM+LDDHFLKASESAHDVSKMLEATRLHFHSNFADNRGHID
Sbjct: 241 PGSSEGKRIVKGSINLLQIFMELDDHFLKASESAHDVSKMLEATRLHFHSNFADNRGHID 300

Query: 301 HSARVMRVITWNRSFRGLPNNDDLNDGFDTEENETHATVLDKLLAWEKKLFEEVKAGEIM 360
           HSARVMRVITWNRSFRGLPNNDDLNDGFDTEENETHATVLDKLLAWEKKLFEEVKAGEIM
Sbjct: 301 HSARVMRVITWNRSFRGLPNNDDLNDGFDTEENETHATVLDKLLAWEKKLFEEVKAGEIM 360

Query: 361 KFEYQKKVAALNKLKKKGSNFEAIEKAKATVSHLHTRYIVDMQSMDSTVSEINRIRDEQL 420
           KFEYQKKVAALNKLKKKGSNFEAIEKAKATVSHLHTRYIVDMQSMDSTVSEINRIRDEQL
Sbjct: 361 KFEYQKKVAALNKLKKKGSNFEAIEKAKATVSHLHTRYIVDMQSMDSTVSEINRIRDEQL 420

Query: 421 YPKLVHLIIGMASMWETMHFHHGSQLKVVAALRMLDISQSPKETSDHHHERTVQLWAVVQ 480
           YPKLVHLI GMASMWETMHFHHGSQLK VAALRMLDISQSPKETSDHHHERTVQLWAVVQ
Sbjct: 421 YPKLVHLINGMASMWETMHFHHGSQLKAVAALRMLDISQSPKETSDHHHERTVQLWAVVQ 480

Query: 481 EWHSQLEKLVNRQKDYIKALSNWLRLNLIPTESSLKEKVSSPPRVRSPPIQILLHAWQDH 540
           EWHSQLEKLVNRQKDYIKALSNWLRLNLIPTESSLKEKVSSPPRVRSPPIQILLHAWQDH
Sbjct: 481 EWHSQLEKLVNRQKDYIKALSNWLRLNLIPTESSLKEKVSSPPRVRSPPIQILLHAWQDH 540

Query: 541 LEKLPDEVLRNAIFTFATVIHTIMQSQEEEMKLKLKCQETEKELARKSKQFKDWQKKYVQ 600
           LEKLPDEVLRNAIFTFATVIHTIMQSQEEEMKLKLKCQETEKELARKSKQFKDWQKKYVQ
Sbjct: 541 LEKLPDEVLRNAIFTFATVIHTIMQSQEEEMKLKLKCQETEKELARKSKQFKDWQKKYVQ 600

Query: 601 RRGSNADEVDMEEPADKDAIAERQAAVEAVEKKLEEEREEHQKLCLHVREKSLGSLKNQL 660
           RRGSNADEVDMEEPADKDAIAERQAAVEAVEKKLEEEREEHQKLCLHVREKSLGSLKNQL
Sbjct: 601 RRGSNADEVDMEEPADKDAIAERQAAVEAVEKKLEEEREEHQKLCLHVREKSLGSLKNQL 660

Query: 661 PELFRALFEFSLACSRMYRHLKSISQPMPNGPQNQTTTLG 701
           PELFRALFEFSLACSRMYRHLKSISQPMPNGPQNQTTT G
Sbjct: 661 PELFRALFEFSLACSRMYRHLKSISQPMPNGPQNQTTTQG 700

BLAST of Chy1G020640 vs. ExPASy TrEMBL
Match: A0A1S4E3Q8 (uncharacterized protein LOC103500575 OS=Cucumis melo OX=3656 GN=LOC103500575 PE=4 SV=1)

HSP 1 Score: 1318.5 bits (3411), Expect = 0.0e+00
Identity = 680/700 (97.14%), Postives = 685/700 (97.86%), Query Frame = 0

Query: 1   MGCSQSKIENEEVVSRCKDRKMFMKDAVTARNAFAAAHSSYAMSLKNTGAVLSDYAHGEG 60
           MGCSQSKIENEEVVSRCKDRKMFMKDAVTARNAFAAAHSSYAMSLKNTGAVLSDYAHGEG
Sbjct: 1   MGCSQSKIENEEVVSRCKDRKMFMKDAVTARNAFAAAHSSYAMSLKNTGAVLSDYAHGEG 60

Query: 61  PPAPSSLPGSAVVQSAAAAGYNSLPPPPPPLPGSPGMPLEIKASKVEPKRVEPAIQEVDE 120
           PPAPSSLPGSAVVQSA AAGYNSLPPPPPPLPGSPGM LEIKASKVEPKRVEP IQEVDE
Sbjct: 61  PPAPSSLPGSAVVQSAVAAGYNSLPPPPPPLPGSPGMSLEIKASKVEPKRVEPVIQEVDE 120

Query: 121 NDFEIECSVGPLRRRRSNRDGSGRGGRAGPGELAEEENGPPPPFPPSSENRRVPVPSPQD 180
           NDFEIECSVGPLRRRRSNRDGSGRGGR GPGELAEEENGPP PFP S E+RRVPVPSPQD
Sbjct: 121 NDFEIECSVGPLRRRRSNRDGSGRGGRTGPGELAEEENGPPLPFPASGESRRVPVPSPQD 180

Query: 181 STYDYLFSVDNMPAPTLSGVEDFGANTETVERRAAMEKSGEEPPSSSAGKTSKKMKQVGY 240
           STYDYLFSVDNMPAPTLSGVEDFGANTETVERRAAMEKSGEEPPSSSAGKTSKKMKQVGY
Sbjct: 181 STYDYLFSVDNMPAPTLSGVEDFGANTETVERRAAMEKSGEEPPSSSAGKTSKKMKQVGY 240

Query: 241 PGSSEGKRIVQGNINLLQIFMDLDDHFLKASESAHDVSKMLEATRLHFHSNFADNRGHID 300
           PGSSEGKRIV+GNINLLQIFM+LDDHFLKASESAHDVSKMLEATRLHFHSNFADNRGHID
Sbjct: 241 PGSSEGKRIVKGNINLLQIFMELDDHFLKASESAHDVSKMLEATRLHFHSNFADNRGHID 300

Query: 301 HSARVMRVITWNRSFRGLPNNDDLNDGFDTEENETHATVLDKLLAWEKKLFEEVKAGEIM 360
           HSARVMRVITWNRSFRGLPNNDDLND FDTEENETHATVLDKLLAWEKKLFEEVKAGEIM
Sbjct: 301 HSARVMRVITWNRSFRGLPNNDDLNDDFDTEENETHATVLDKLLAWEKKLFEEVKAGEIM 360

Query: 361 KFEYQKKVAALNKLKKKGSNFEAIEKAKATVSHLHTRYIVDMQSMDSTVSEINRIRDEQL 420
           KFEYQKKVAALNKLKKKGSNFEAIEKAKATVSHLHTRYIVDMQSMDSTVSEINRIRDEQL
Sbjct: 361 KFEYQKKVAALNKLKKKGSNFEAIEKAKATVSHLHTRYIVDMQSMDSTVSEINRIRDEQL 420

Query: 421 YPKLVHLIIGMASMWETMHFHHGSQLKVVAALRMLDISQSPKETSDHHHERTVQLWAVVQ 480
           YPKLV LI GMASMWETMHFHHGSQLKVVAALRMLDISQSPKETSDHHHERTVQLWAVVQ
Sbjct: 421 YPKLVQLINGMASMWETMHFHHGSQLKVVAALRMLDISQSPKETSDHHHERTVQLWAVVQ 480

Query: 481 EWHSQLEKLVNRQKDYIKALSNWLRLNLIPTESSLKEKVSSPPRVRSPPIQILLHAWQDH 540
           EWHSQLEKLVNRQKDYIKALSNWLRLNLIPTESSLKEKVSSPPRVRSPPIQ LLHAWQDH
Sbjct: 481 EWHSQLEKLVNRQKDYIKALSNWLRLNLIPTESSLKEKVSSPPRVRSPPIQSLLHAWQDH 540

Query: 541 LEKLPDEVLRNAIFTFATVIHTIMQSQEEEMKLKLKCQETEKELARKSKQFKDWQKKYVQ 600
           LEKLPDEVLRN IFTFATVIHTIMQSQEEEMKLKLKCQETEKELARKSKQFKDWQKKYVQ
Sbjct: 541 LEKLPDEVLRNTIFTFATVIHTIMQSQEEEMKLKLKCQETEKELARKSKQFKDWQKKYVQ 600

Query: 601 RRGSNADEVDMEEPADKDAIAERQAAVEAVEKKLEEEREEHQKLCLHVREKSLGSLKNQL 660
           RRGSNADEVDMEEP DKDAIAERQAAVEAVEK+LEEEREEHQKLCLHVREKSLGSLKNQL
Sbjct: 601 RRGSNADEVDMEEPGDKDAIAERQAAVEAVEKRLEEEREEHQKLCLHVREKSLGSLKNQL 660

Query: 661 PELFRALFEFSLACSRMYRHLKSISQPMPNGPQNQTTTLG 701
           PELFRALFEFSLACSRMYRHLKSISQP+PNG QNQTTT G
Sbjct: 661 PELFRALFEFSLACSRMYRHLKSISQPIPNGSQNQTTTQG 700

BLAST of Chy1G020640 vs. ExPASy TrEMBL
Match: A0A5A7UW13 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold242G00130 PE=4 SV=1)

HSP 1 Score: 1314.7 bits (3401), Expect = 0.0e+00
Identity = 679/700 (97.00%), Postives = 684/700 (97.71%), Query Frame = 0

Query: 1   MGCSQSKIENEEVVSRCKDRKMFMKDAVTARNAFAAAHSSYAMSLKNTGAVLSDYAHGEG 60
           MGCSQSKIENEEVVSRCKDRKMFMKDAVTARNAFAAAHSSYAMSLKNTGAVLSDYAHGEG
Sbjct: 1   MGCSQSKIENEEVVSRCKDRKMFMKDAVTARNAFAAAHSSYAMSLKNTGAVLSDYAHGEG 60

Query: 61  PPAPSSLPGSAVVQSAAAAGYNSLPPPPPPLPGSPGMPLEIKASKVEPKRVEPAIQEVDE 120
           PPAPSSLPGSAVVQSA AAGYNSLPPPPPPLPGSPGM LEIKASKVEPKRVEP IQEVDE
Sbjct: 61  PPAPSSLPGSAVVQSAVAAGYNSLPPPPPPLPGSPGMSLEIKASKVEPKRVEPVIQEVDE 120

Query: 121 NDFEIECSVGPLRRRRSNRDGSGRGGRAGPGELAEEENGPPPPFPPSSENRRVPVPSPQD 180
           NDFEIECSVGPLRRRRSNRDGSGRGGR GPGELAEEENGPP PFP S E+RRVPVPSPQD
Sbjct: 121 NDFEIECSVGPLRRRRSNRDGSGRGGRTGPGELAEEENGPPLPFPASGESRRVPVPSPQD 180

Query: 181 STYDYLFSVDNMPAPTLSGVEDFGANTETVERRAAMEKSGEEPPSSSAGKTSKKMKQVGY 240
           STYDYLFSVDNMPAPTLSGVEDFGANTETVERRAAMEKSGEE PSSSAGKTSKKMKQVGY
Sbjct: 181 STYDYLFSVDNMPAPTLSGVEDFGANTETVERRAAMEKSGEELPSSSAGKTSKKMKQVGY 240

Query: 241 PGSSEGKRIVQGNINLLQIFMDLDDHFLKASESAHDVSKMLEATRLHFHSNFADNRGHID 300
           PGSSEGKRIV+GNINLLQIFM+LDDHFLKASESAHDVSKMLEATRLHFHSNFADNRGHID
Sbjct: 241 PGSSEGKRIVKGNINLLQIFMELDDHFLKASESAHDVSKMLEATRLHFHSNFADNRGHID 300

Query: 301 HSARVMRVITWNRSFRGLPNNDDLNDGFDTEENETHATVLDKLLAWEKKLFEEVKAGEIM 360
           HSARVMRVITWNRSFRGLPNNDDLND FDTEENETHATVLDKLLAWEKKLFEEVKAGEIM
Sbjct: 301 HSARVMRVITWNRSFRGLPNNDDLNDDFDTEENETHATVLDKLLAWEKKLFEEVKAGEIM 360

Query: 361 KFEYQKKVAALNKLKKKGSNFEAIEKAKATVSHLHTRYIVDMQSMDSTVSEINRIRDEQL 420
           KFEYQKKVAALNKLKKKGSNFEAIEKAKATVSHLHTRYIVDMQSMDSTVSEINRIRDEQL
Sbjct: 361 KFEYQKKVAALNKLKKKGSNFEAIEKAKATVSHLHTRYIVDMQSMDSTVSEINRIRDEQL 420

Query: 421 YPKLVHLIIGMASMWETMHFHHGSQLKVVAALRMLDISQSPKETSDHHHERTVQLWAVVQ 480
           YPKLV LI GMASMWETMHFHHGSQLKVVAALRMLDISQSPKETSDHHHERTVQLWAVVQ
Sbjct: 421 YPKLVQLINGMASMWETMHFHHGSQLKVVAALRMLDISQSPKETSDHHHERTVQLWAVVQ 480

Query: 481 EWHSQLEKLVNRQKDYIKALSNWLRLNLIPTESSLKEKVSSPPRVRSPPIQILLHAWQDH 540
           EWHSQLEKLVNRQKDYIKALSNWLRLNLIPTESSLKEKVSSPPRVRSPPIQ LLHAWQDH
Sbjct: 481 EWHSQLEKLVNRQKDYIKALSNWLRLNLIPTESSLKEKVSSPPRVRSPPIQSLLHAWQDH 540

Query: 541 LEKLPDEVLRNAIFTFATVIHTIMQSQEEEMKLKLKCQETEKELARKSKQFKDWQKKYVQ 600
           LEKLPDEVLRN IFTFATVIHTIMQSQEEEMKLKLKCQETEKELARKSKQFKDWQKKYVQ
Sbjct: 541 LEKLPDEVLRNTIFTFATVIHTIMQSQEEEMKLKLKCQETEKELARKSKQFKDWQKKYVQ 600

Query: 601 RRGSNADEVDMEEPADKDAIAERQAAVEAVEKKLEEEREEHQKLCLHVREKSLGSLKNQL 660
           RRGSNADEVDMEEP DKDAIAERQAAVEAVEK+LEEEREEHQKLCLHVREKSLGSLKNQL
Sbjct: 601 RRGSNADEVDMEEPGDKDAIAERQAAVEAVEKRLEEEREEHQKLCLHVREKSLGSLKNQL 660

Query: 661 PELFRALFEFSLACSRMYRHLKSISQPMPNGPQNQTTTLG 701
           PELFRALFEFSLACSRMYRHLKSISQP+PNG QNQTTT G
Sbjct: 661 PELFRALFEFSLACSRMYRHLKSISQPIPNGSQNQTTTQG 700

BLAST of Chy1G020640 vs. ExPASy TrEMBL
Match: A0A6J1GQ84 (nitrate regulatory gene2 protein-like OS=Cucurbita moschata OX=3662 GN=LOC111456482 PE=4 SV=1)

HSP 1 Score: 1197.6 bits (3097), Expect = 0.0e+00
Identity = 629/714 (88.10%), Postives = 654/714 (91.60%), Query Frame = 0

Query: 1   MGCSQSKIENEEVVSRCKDRKMFMKDAVTARNAFAAAHSSYAMSLKNTGAVLSDYAHGEG 60
           MGCSQSKIENEEVVSRCKDRKMFMK+AV ARNAFAAAHSSYAMSLKNTGAVLSDYAHGE 
Sbjct: 1   MGCSQSKIENEEVVSRCKDRKMFMKEAVAARNAFAAAHSSYAMSLKNTGAVLSDYAHGEV 60

Query: 61  PPAPSSLPGSAVVQS---AAAAGYNSLPPPPPPLPGSPGMPL-------EIKASKVEPKR 120
           PPA SSLPG AV QS   AA+A YNSLPPPPPPLPGSPGMPL       EIKASKVEPKR
Sbjct: 61  PPAASSLPGVAVAQSAAVAASASYNSLPPPPPPLPGSPGMPLRNGTSMFEIKASKVEPKR 120

Query: 121 VEPAIQEVDENDFEIECSVGPLRRRRSNRDGSGRGGRAGPGELAEEENGPPPPFPPS--- 180
           VE  I+EVDENDFEIECSVGPL RRRSNR+G GRGGR G GELAEEENGPPPP PPS   
Sbjct: 121 VETVIEEVDENDFEIECSVGPL-RRRSNREGGGRGGRTGLGELAEEENGPPPPLPPSLNR 180

Query: 181 ----SENRRVPVPSPQDSTYDYLFSVDNMPAPTLSGVEDFGANTETVERRAAMEKSGEEP 240
               +ENRR   PSPQD+TYDYLFSV+NMPAPTLS VEDFG NTE +ERRAA+EKSG E 
Sbjct: 181 PPPPNENRRAHSPSPQDATYDYLFSVENMPAPTLSSVEDFGTNTEAIERRAAVEKSGGEL 240

Query: 241 PSSSAGKTSKKMKQVGYPGSSEGKRIVQGNINLLQIFMDLDDHFLKASESAHDVSKMLEA 300
           PSSSAGKTSKK+KQVG+P S EGKR V+GN +LLQIFM+LDDHFLKASESAHDVSKMLEA
Sbjct: 241 PSSSAGKTSKKLKQVGFPCSIEGKRAVKGNTSLLQIFMELDDHFLKASESAHDVSKMLEA 300

Query: 301 TRLHFHSNFADNRGHIDHSARVMRVITWNRSFRGLPNNDDLNDGFDTEENETHATVLDKL 360
           TRLHFHSNFADNRGHIDHSARVMRVITWNRSFRGLPNNDDLND FDTEE+ETHATVLDKL
Sbjct: 301 TRLHFHSNFADNRGHIDHSARVMRVITWNRSFRGLPNNDDLNDDFDTEEHETHATVLDKL 360

Query: 361 LAWEKKLFEEVKAGEIMKFEYQKKVAALNKLKKKGSNFEAIEKAKATVSHLHTRYIVDMQ 420
           LAWEKKLFEEVKAGE+MKFEYQKKVAALNKLKKKGSNFEAIEKAKATVSHLHTRYIVDMQ
Sbjct: 361 LAWEKKLFEEVKAGEVMKFEYQKKVAALNKLKKKGSNFEAIEKAKATVSHLHTRYIVDMQ 420

Query: 421 SMDSTVSEINRIRDEQLYPKLVHLIIGMASMWETMHFHHGSQLKVVAALRMLDISQSPKE 480
           SMDSTVSEINRIRDEQLYPKLV L+ GMASMWE MHFHHG QLK VAALR LDI QSPKE
Sbjct: 421 SMDSTVSEINRIRDEQLYPKLVQLVNGMASMWEIMHFHHGGQLKAVAALRTLDIPQSPKE 480

Query: 481 TSDHHHERTVQLWAVVQEWHSQLEKLVNRQKDYIKALSNWLRLNLIPTESSLKEKVSSPP 540
           TSDHHHERTVQLWAVVQEWHSQLEKLV RQK+YIKALSNWLRLNLIPTESSLKEKVSSPP
Sbjct: 481 TSDHHHERTVQLWAVVQEWHSQLEKLVTRQKEYIKALSNWLRLNLIPTESSLKEKVSSPP 540

Query: 541 RVRSPPIQILLHAWQDHLEKLPDEVLRNAIFTFATVIHTIMQSQEEEMKLKLKCQETEKE 600
           RVRSPPIQ LLH WQDHLEKLPDEVLRNAIFTFATVI+TI+QSQEEEMKLK+KCQETEKE
Sbjct: 541 RVRSPPIQSLLHVWQDHLEKLPDEVLRNAIFTFATVINTIVQSQEEEMKLKVKCQETEKE 600

Query: 601 LARKSKQFKDWQKKYVQRRGSNADEVDMEEPADKDAIAERQAAVEAVEKKLEEEREEHQK 660
           LARKSKQFKDWQKKYVQRR  NADE + EE  DKDAIAERQAAVEAVEK+LEEEREEHQK
Sbjct: 601 LARKSKQFKDWQKKYVQRRAPNADEANQEETGDKDAIAERQAAVEAVEKRLEEEREEHQK 660

Query: 661 LCLHVREKSLGSLKNQLPELFRALFEFSLACSRMYRHLKSISQPMPNGPQNQTT 698
           LCLHVREKSLGSLKNQLPELFRALFEFSLACSRMYRHLKSISQP+PN PQ+QTT
Sbjct: 661 LCLHVREKSLGSLKNQLPELFRALFEFSLACSRMYRHLKSISQPLPNRPQSQTT 713

BLAST of Chy1G020640 vs. ExPASy TrEMBL
Match: A0A6J1JYE9 (nitrate regulatory gene2 protein-like OS=Cucurbita maxima OX=3661 GN=LOC111488577 PE=4 SV=1)

HSP 1 Score: 1191.4 bits (3081), Expect = 0.0e+00
Identity = 627/714 (87.82%), Postives = 652/714 (91.32%), Query Frame = 0

Query: 1   MGCSQSKIENEEVVSRCKDRKMFMKDAVTARNAFAAAHSSYAMSLKNTGAVLSDYAHGEG 60
           MGCSQSKIENEEVV RCKDRKMFMK+AV ARNAFAAAHSSYAMSLKNTGAVLSDYAHGE 
Sbjct: 1   MGCSQSKIENEEVVCRCKDRKMFMKEAVAARNAFAAAHSSYAMSLKNTGAVLSDYAHGEV 60

Query: 61  PPAPSSLPGSAVVQS---AAAAGYNSLPPPPPPLPGSPGMPL-------EIKASKVEPKR 120
           PPA SSLPG AV QS   AA+A YNSLPPPPPPLPGSPGMPL       EIKASKVE KR
Sbjct: 61  PPAASSLPGVAVAQSAVVAASASYNSLPPPPPPLPGSPGMPLRNGTSMFEIKASKVESKR 120

Query: 121 VEPAIQEVDENDFEIECSVGPLRRRRSNRDGSGRGGRAGPGELAEEENGPPPPFPPS--- 180
           VEP I+EVDENDFEIECSVGPL RRRSNR+G GRG R G GELAEEENGPPPP PPS   
Sbjct: 121 VEPVIEEVDENDFEIECSVGPL-RRRSNREGGGRGSRTGLGELAEEENGPPPPLPPSLNL 180

Query: 181 ----SENRRVPVPSPQDSTYDYLFSVDNMPAPTLSGVEDFGANTETVERRAAMEKSGEEP 240
               +ENRRV  PSPQDSTYDYLFSV+NMPAPTLS VEDFG NTE +ERRAA E+SG E 
Sbjct: 181 PPPPNENRRVHSPSPQDSTYDYLFSVENMPAPTLSSVEDFGTNTEAIERRAAAEESGREL 240

Query: 241 PSSSAGKTSKKMKQVGYPGSSEGKRIVQGNINLLQIFMDLDDHFLKASESAHDVSKMLEA 300
           PSSSAGKTSKK+KQVG+P S EGKR V+GN +LLQIFM+LDDHFLKASESAHDVSKMLEA
Sbjct: 241 PSSSAGKTSKKLKQVGFPCSIEGKRAVKGNTSLLQIFMELDDHFLKASESAHDVSKMLEA 300

Query: 301 TRLHFHSNFADNRGHIDHSARVMRVITWNRSFRGLPNNDDLNDGFDTEENETHATVLDKL 360
           TRLHFHSNFADNRGHIDHSARVMRVITWNRSFRGLPNNDDLND FDTEE+ETHATVLDKL
Sbjct: 301 TRLHFHSNFADNRGHIDHSARVMRVITWNRSFRGLPNNDDLNDDFDTEEHETHATVLDKL 360

Query: 361 LAWEKKLFEEVKAGEIMKFEYQKKVAALNKLKKKGSNFEAIEKAKATVSHLHTRYIVDMQ 420
           LAWEKKLFEEVKAGE+MKFEYQKKVAALNKLKKKGSNFEAIEKAKATVSHLHTRYIVDMQ
Sbjct: 361 LAWEKKLFEEVKAGEVMKFEYQKKVAALNKLKKKGSNFEAIEKAKATVSHLHTRYIVDMQ 420

Query: 421 SMDSTVSEINRIRDEQLYPKLVHLIIGMASMWETMHFHHGSQLKVVAALRMLDISQSPKE 480
           SMDSTVSEINRIRDEQLYPKLV L+ GMASMWE MHFHHGSQLK VAALR LDI QSPKE
Sbjct: 421 SMDSTVSEINRIRDEQLYPKLVQLVNGMASMWEIMHFHHGSQLKAVAALRTLDIPQSPKE 480

Query: 481 TSDHHHERTVQLWAVVQEWHSQLEKLVNRQKDYIKALSNWLRLNLIPTESSLKEKVSSPP 540
           TSDHHHERTVQLWAVVQEWHSQLEKLV RQK+YIKALSNWLRLNLIPTESSLKEKVSSPP
Sbjct: 481 TSDHHHERTVQLWAVVQEWHSQLEKLVTRQKEYIKALSNWLRLNLIPTESSLKEKVSSPP 540

Query: 541 RVRSPPIQILLHAWQDHLEKLPDEVLRNAIFTFATVIHTIMQSQEEEMKLKLKCQETEKE 600
           RVRSPPIQ LLH WQDHLEKLPDEVLRNAIFTFATVI+TI+QSQEEEMKLK KCQETEKE
Sbjct: 541 RVRSPPIQSLLHVWQDHLEKLPDEVLRNAIFTFATVINTIVQSQEEEMKLKAKCQETEKE 600

Query: 601 LARKSKQFKDWQKKYVQRRGSNADEVDMEEPADKDAIAERQAAVEAVEKKLEEEREEHQK 660
           LARKSKQFKDWQKKYVQRR  N++E + EE  DKDAIAERQAAVEAVEK+LEEEREEHQK
Sbjct: 601 LARKSKQFKDWQKKYVQRRAPNSNEANPEETGDKDAIAERQAAVEAVEKRLEEEREEHQK 660

Query: 661 LCLHVREKSLGSLKNQLPELFRALFEFSLACSRMYRHLKSISQPMPNGPQNQTT 698
           LCLHVREKSLGSLKNQLPELFRALFEFSLACSRMYRHLKSISQP+PN PQ+QTT
Sbjct: 661 LCLHVREKSLGSLKNQLPELFRALFEFSLACSRMYRHLKSISQPLPNRPQSQTT 713

BLAST of Chy1G020640 vs. NCBI nr
Match: XP_004141776.1 (nitrate regulatory gene2 protein [Cucumis sativus] >XP_011659565.1 nitrate regulatory gene2 protein [Cucumis sativus] >XP_031744907.1 nitrate regulatory gene2 protein [Cucumis sativus] >KGN45358.1 hypothetical protein Csa_015836 [Cucumis sativus])

HSP 1 Score: 1339 bits (3466), Expect = 0.0
Identity = 691/700 (98.71%), Postives = 695/700 (99.29%), Query Frame = 0

Query: 1   MGCSQSKIENEEVVSRCKDRKMFMKDAVTARNAFAAAHSSYAMSLKNTGAVLSDYAHGEG 60
           MGCSQSKIENEEVVSRCKDRKMFMKDAVTARNAFAAAHSSYAMSLKNTGAVLSDYAHGEG
Sbjct: 1   MGCSQSKIENEEVVSRCKDRKMFMKDAVTARNAFAAAHSSYAMSLKNTGAVLSDYAHGEG 60

Query: 61  PPAPSSLPGSAVVQSAAAAGYNSLPPPPPPLPGSPGMPLEIKASKVEPKRVEPAIQEVDE 120
           PPAPSSLPGS+VVQSAAAAGYNSLPPPPPPLPGSPGMPLEIKASKVEPKRVEP IQEVDE
Sbjct: 61  PPAPSSLPGSSVVQSAAAAGYNSLPPPPPPLPGSPGMPLEIKASKVEPKRVEPVIQEVDE 120

Query: 121 NDFEIECSVGPLRRRRSNRDGSGRGGRAGPGELAEEENGPPPPFPPSSENRRVPVPSPQD 180
           NDFEIECSVGPLRRRRSNRDGSGRGGRAGPGELAEEENGPPPPFPPSSENRRVPVPSPQD
Sbjct: 121 NDFEIECSVGPLRRRRSNRDGSGRGGRAGPGELAEEENGPPPPFPPSSENRRVPVPSPQD 180

Query: 181 STYDYLFSVDNMPAPTLSGVEDFGANTETVERRAAMEKSGEEPPSSSAGKTSKKMKQVGY 240
           STYDYLFSVDNMPAPTLSGVEDFGANTETVERRAA EKSGEEPPSSSAGKTSKKMKQVGY
Sbjct: 181 STYDYLFSVDNMPAPTLSGVEDFGANTETVERRAATEKSGEEPPSSSAGKTSKKMKQVGY 240

Query: 241 PGSSEGKRIVQGNINLLQIFMDLDDHFLKASESAHDVSKMLEATRLHFHSNFADNRGHID 300
           PGSSEGKRIV+G+INLLQIFM+LDDHFLKASESAHDVSKMLEATRLHFHSNFADNRGHID
Sbjct: 241 PGSSEGKRIVKGSINLLQIFMELDDHFLKASESAHDVSKMLEATRLHFHSNFADNRGHID 300

Query: 301 HSARVMRVITWNRSFRGLPNNDDLNDGFDTEENETHATVLDKLLAWEKKLFEEVKAGEIM 360
           HSARVMRVITWNRSFRGLPNNDDLNDGFDTEENETHATVLDKLLAWEKKLFEEVKAGEIM
Sbjct: 301 HSARVMRVITWNRSFRGLPNNDDLNDGFDTEENETHATVLDKLLAWEKKLFEEVKAGEIM 360

Query: 361 KFEYQKKVAALNKLKKKGSNFEAIEKAKATVSHLHTRYIVDMQSMDSTVSEINRIRDEQL 420
           KFEYQKKVAALNKLKKKGSNFEAIEKAKATVSHLHTRYIVDMQSMDSTVSEINRIRDEQL
Sbjct: 361 KFEYQKKVAALNKLKKKGSNFEAIEKAKATVSHLHTRYIVDMQSMDSTVSEINRIRDEQL 420

Query: 421 YPKLVHLIIGMASMWETMHFHHGSQLKVVAALRMLDISQSPKETSDHHHERTVQLWAVVQ 480
           YPKLVHLI GMASMWETMHFHHGSQLK VAALRMLDISQSPKETSDHHHERTVQLWAVVQ
Sbjct: 421 YPKLVHLINGMASMWETMHFHHGSQLKAVAALRMLDISQSPKETSDHHHERTVQLWAVVQ 480

Query: 481 EWHSQLEKLVNRQKDYIKALSNWLRLNLIPTESSLKEKVSSPPRVRSPPIQILLHAWQDH 540
           EWHSQLEKLVNRQKDYIKALSNWLRLNLIPTESSLKEKVSSPPRVRSPPIQILLHAWQDH
Sbjct: 481 EWHSQLEKLVNRQKDYIKALSNWLRLNLIPTESSLKEKVSSPPRVRSPPIQILLHAWQDH 540

Query: 541 LEKLPDEVLRNAIFTFATVIHTIMQSQEEEMKLKLKCQETEKELARKSKQFKDWQKKYVQ 600
           LEKLPDEVLRNAIFTFATVIHTIMQSQEEEMKLKLKCQETEKELARKSKQFKDWQKKYVQ
Sbjct: 541 LEKLPDEVLRNAIFTFATVIHTIMQSQEEEMKLKLKCQETEKELARKSKQFKDWQKKYVQ 600

Query: 601 RRGSNADEVDMEEPADKDAIAERQAAVEAVEKKLEEEREEHQKLCLHVREKSLGSLKNQL 660
           RRGSNADEVDMEEPADKDAIAERQAAVEAVEKKLEEEREEHQKLCLHVREKSLGSLKNQL
Sbjct: 601 RRGSNADEVDMEEPADKDAIAERQAAVEAVEKKLEEEREEHQKLCLHVREKSLGSLKNQL 660

Query: 661 PELFRALFEFSLACSRMYRHLKSISQPMPNGPQNQTTTLG 700
           PELFRALFEFSLACSRMYRHLKSISQPMPNGPQNQTTT G
Sbjct: 661 PELFRALFEFSLACSRMYRHLKSISQPMPNGPQNQTTTQG 700

BLAST of Chy1G020640 vs. NCBI nr
Match: XP_008462152.1 (PREDICTED: uncharacterized protein LOC103500575 [Cucumis melo] >XP_016902857.1 PREDICTED: uncharacterized protein LOC103500575 [Cucumis melo])

HSP 1 Score: 1313 bits (3397), Expect = 0.0
Identity = 680/700 (97.14%), Postives = 685/700 (97.86%), Query Frame = 0

Query: 1   MGCSQSKIENEEVVSRCKDRKMFMKDAVTARNAFAAAHSSYAMSLKNTGAVLSDYAHGEG 60
           MGCSQSKIENEEVVSRCKDRKMFMKDAVTARNAFAAAHSSYAMSLKNTGAVLSDYAHGEG
Sbjct: 1   MGCSQSKIENEEVVSRCKDRKMFMKDAVTARNAFAAAHSSYAMSLKNTGAVLSDYAHGEG 60

Query: 61  PPAPSSLPGSAVVQSAAAAGYNSLPPPPPPLPGSPGMPLEIKASKVEPKRVEPAIQEVDE 120
           PPAPSSLPGSAVVQSA AAGYNSLPPPPPPLPGSPGM LEIKASKVEPKRVEP IQEVDE
Sbjct: 61  PPAPSSLPGSAVVQSAVAAGYNSLPPPPPPLPGSPGMSLEIKASKVEPKRVEPVIQEVDE 120

Query: 121 NDFEIECSVGPLRRRRSNRDGSGRGGRAGPGELAEEENGPPPPFPPSSENRRVPVPSPQD 180
           NDFEIECSVGPLRRRRSNRDGSGRGGR GPGELAEEENGPP PFP S E+RRVPVPSPQD
Sbjct: 121 NDFEIECSVGPLRRRRSNRDGSGRGGRTGPGELAEEENGPPLPFPASGESRRVPVPSPQD 180

Query: 181 STYDYLFSVDNMPAPTLSGVEDFGANTETVERRAAMEKSGEEPPSSSAGKTSKKMKQVGY 240
           STYDYLFSVDNMPAPTLSGVEDFGANTETVERRAAMEKSGEEPPSSSAGKTSKKMKQVGY
Sbjct: 181 STYDYLFSVDNMPAPTLSGVEDFGANTETVERRAAMEKSGEEPPSSSAGKTSKKMKQVGY 240

Query: 241 PGSSEGKRIVQGNINLLQIFMDLDDHFLKASESAHDVSKMLEATRLHFHSNFADNRGHID 300
           PGSSEGKRIV+GNINLLQIFM+LDDHFLKASESAHDVSKMLEATRLHFHSNFADNRGHID
Sbjct: 241 PGSSEGKRIVKGNINLLQIFMELDDHFLKASESAHDVSKMLEATRLHFHSNFADNRGHID 300

Query: 301 HSARVMRVITWNRSFRGLPNNDDLNDGFDTEENETHATVLDKLLAWEKKLFEEVKAGEIM 360
           HSARVMRVITWNRSFRGLPNNDDLND FDTEENETHATVLDKLLAWEKKLFEEVKAGEIM
Sbjct: 301 HSARVMRVITWNRSFRGLPNNDDLNDDFDTEENETHATVLDKLLAWEKKLFEEVKAGEIM 360

Query: 361 KFEYQKKVAALNKLKKKGSNFEAIEKAKATVSHLHTRYIVDMQSMDSTVSEINRIRDEQL 420
           KFEYQKKVAALNKLKKKGSNFEAIEKAKATVSHLHTRYIVDMQSMDSTVSEINRIRDEQL
Sbjct: 361 KFEYQKKVAALNKLKKKGSNFEAIEKAKATVSHLHTRYIVDMQSMDSTVSEINRIRDEQL 420

Query: 421 YPKLVHLIIGMASMWETMHFHHGSQLKVVAALRMLDISQSPKETSDHHHERTVQLWAVVQ 480
           YPKLV LI GMASMWETMHFHHGSQLKVVAALRMLDISQSPKETSDHHHERTVQLWAVVQ
Sbjct: 421 YPKLVQLINGMASMWETMHFHHGSQLKVVAALRMLDISQSPKETSDHHHERTVQLWAVVQ 480

Query: 481 EWHSQLEKLVNRQKDYIKALSNWLRLNLIPTESSLKEKVSSPPRVRSPPIQILLHAWQDH 540
           EWHSQLEKLVNRQKDYIKALSNWLRLNLIPTESSLKEKVSSPPRVRSPPIQ LLHAWQDH
Sbjct: 481 EWHSQLEKLVNRQKDYIKALSNWLRLNLIPTESSLKEKVSSPPRVRSPPIQSLLHAWQDH 540

Query: 541 LEKLPDEVLRNAIFTFATVIHTIMQSQEEEMKLKLKCQETEKELARKSKQFKDWQKKYVQ 600
           LEKLPDEVLRN IFTFATVIHTIMQSQEEEMKLKLKCQETEKELARKSKQFKDWQKKYVQ
Sbjct: 541 LEKLPDEVLRNTIFTFATVIHTIMQSQEEEMKLKLKCQETEKELARKSKQFKDWQKKYVQ 600

Query: 601 RRGSNADEVDMEEPADKDAIAERQAAVEAVEKKLEEEREEHQKLCLHVREKSLGSLKNQL 660
           RRGSNADEVDMEEP DKDAIAERQAAVEAVEK+LEEEREEHQKLCLHVREKSLGSLKNQL
Sbjct: 601 RRGSNADEVDMEEPGDKDAIAERQAAVEAVEKRLEEEREEHQKLCLHVREKSLGSLKNQL 660

Query: 661 PELFRALFEFSLACSRMYRHLKSISQPMPNGPQNQTTTLG 700
           PELFRALFEFSLACSRMYRHLKSISQP+PNG QNQTTT G
Sbjct: 661 PELFRALFEFSLACSRMYRHLKSISQPIPNGSQNQTTTQG 700

BLAST of Chy1G020640 vs. NCBI nr
Match: KAA0059290.1 (uncharacterized protein E6C27_scaffold242G00130 [Cucumis melo var. makuwa])

HSP 1 Score: 1309 bits (3387), Expect = 0.0
Identity = 679/700 (97.00%), Postives = 684/700 (97.71%), Query Frame = 0

Query: 1   MGCSQSKIENEEVVSRCKDRKMFMKDAVTARNAFAAAHSSYAMSLKNTGAVLSDYAHGEG 60
           MGCSQSKIENEEVVSRCKDRKMFMKDAVTARNAFAAAHSSYAMSLKNTGAVLSDYAHGEG
Sbjct: 1   MGCSQSKIENEEVVSRCKDRKMFMKDAVTARNAFAAAHSSYAMSLKNTGAVLSDYAHGEG 60

Query: 61  PPAPSSLPGSAVVQSAAAAGYNSLPPPPPPLPGSPGMPLEIKASKVEPKRVEPAIQEVDE 120
           PPAPSSLPGSAVVQSA AAGYNSLPPPPPPLPGSPGM LEIKASKVEPKRVEP IQEVDE
Sbjct: 61  PPAPSSLPGSAVVQSAVAAGYNSLPPPPPPLPGSPGMSLEIKASKVEPKRVEPVIQEVDE 120

Query: 121 NDFEIECSVGPLRRRRSNRDGSGRGGRAGPGELAEEENGPPPPFPPSSENRRVPVPSPQD 180
           NDFEIECSVGPLRRRRSNRDGSGRGGR GPGELAEEENGPP PFP S E+RRVPVPSPQD
Sbjct: 121 NDFEIECSVGPLRRRRSNRDGSGRGGRTGPGELAEEENGPPLPFPASGESRRVPVPSPQD 180

Query: 181 STYDYLFSVDNMPAPTLSGVEDFGANTETVERRAAMEKSGEEPPSSSAGKTSKKMKQVGY 240
           STYDYLFSVDNMPAPTLSGVEDFGANTETVERRAAMEKSGEE PSSSAGKTSKKMKQVGY
Sbjct: 181 STYDYLFSVDNMPAPTLSGVEDFGANTETVERRAAMEKSGEELPSSSAGKTSKKMKQVGY 240

Query: 241 PGSSEGKRIVQGNINLLQIFMDLDDHFLKASESAHDVSKMLEATRLHFHSNFADNRGHID 300
           PGSSEGKRIV+GNINLLQIFM+LDDHFLKASESAHDVSKMLEATRLHFHSNFADNRGHID
Sbjct: 241 PGSSEGKRIVKGNINLLQIFMELDDHFLKASESAHDVSKMLEATRLHFHSNFADNRGHID 300

Query: 301 HSARVMRVITWNRSFRGLPNNDDLNDGFDTEENETHATVLDKLLAWEKKLFEEVKAGEIM 360
           HSARVMRVITWNRSFRGLPNNDDLND FDTEENETHATVLDKLLAWEKKLFEEVKAGEIM
Sbjct: 301 HSARVMRVITWNRSFRGLPNNDDLNDDFDTEENETHATVLDKLLAWEKKLFEEVKAGEIM 360

Query: 361 KFEYQKKVAALNKLKKKGSNFEAIEKAKATVSHLHTRYIVDMQSMDSTVSEINRIRDEQL 420
           KFEYQKKVAALNKLKKKGSNFEAIEKAKATVSHLHTRYIVDMQSMDSTVSEINRIRDEQL
Sbjct: 361 KFEYQKKVAALNKLKKKGSNFEAIEKAKATVSHLHTRYIVDMQSMDSTVSEINRIRDEQL 420

Query: 421 YPKLVHLIIGMASMWETMHFHHGSQLKVVAALRMLDISQSPKETSDHHHERTVQLWAVVQ 480
           YPKLV LI GMASMWETMHFHHGSQLKVVAALRMLDISQSPKETSDHHHERTVQLWAVVQ
Sbjct: 421 YPKLVQLINGMASMWETMHFHHGSQLKVVAALRMLDISQSPKETSDHHHERTVQLWAVVQ 480

Query: 481 EWHSQLEKLVNRQKDYIKALSNWLRLNLIPTESSLKEKVSSPPRVRSPPIQILLHAWQDH 540
           EWHSQLEKLVNRQKDYIKALSNWLRLNLIPTESSLKEKVSSPPRVRSPPIQ LLHAWQDH
Sbjct: 481 EWHSQLEKLVNRQKDYIKALSNWLRLNLIPTESSLKEKVSSPPRVRSPPIQSLLHAWQDH 540

Query: 541 LEKLPDEVLRNAIFTFATVIHTIMQSQEEEMKLKLKCQETEKELARKSKQFKDWQKKYVQ 600
           LEKLPDEVLRN IFTFATVIHTIMQSQEEEMKLKLKCQETEKELARKSKQFKDWQKKYVQ
Sbjct: 541 LEKLPDEVLRNTIFTFATVIHTIMQSQEEEMKLKLKCQETEKELARKSKQFKDWQKKYVQ 600

Query: 601 RRGSNADEVDMEEPADKDAIAERQAAVEAVEKKLEEEREEHQKLCLHVREKSLGSLKNQL 660
           RRGSNADEVDMEEP DKDAIAERQAAVEAVEK+LEEEREEHQKLCLHVREKSLGSLKNQL
Sbjct: 601 RRGSNADEVDMEEPGDKDAIAERQAAVEAVEKRLEEEREEHQKLCLHVREKSLGSLKNQL 660

Query: 661 PELFRALFEFSLACSRMYRHLKSISQPMPNGPQNQTTTLG 700
           PELFRALFEFSLACSRMYRHLKSISQP+PNG QNQTTT G
Sbjct: 661 PELFRALFEFSLACSRMYRHLKSISQPIPNGSQNQTTTQG 700

BLAST of Chy1G020640 vs. NCBI nr
Match: XP_038899337.1 (protein ROLLING AND ERECT LEAF 2-like [Benincasa hispida])

HSP 1 Score: 1256 bits (3251), Expect = 0.0
Identity = 653/705 (92.62%), Postives = 669/705 (94.89%), Query Frame = 0

Query: 1   MGCSQSKIENEEVVSRCKDRKMFMKDAVTARNAFAAAHSSYAMSLKNTGAVLSDYAHGEG 60
           MGCSQSKIENEEVVSRCKDRKMFMKDAV ARNAFAAAHSSYAMSLKNTGAVLSDYAHGEG
Sbjct: 1   MGCSQSKIENEEVVSRCKDRKMFMKDAVAARNAFAAAHSSYAMSLKNTGAVLSDYAHGEG 60

Query: 61  PPAPSSLPGSAVVQSAAAAGYNSLPPPPPPLPGSPGMPL-------EIKASKVEPKRVEP 120
           PPAPSSLPGSA  QSAAA  YNSLPPPPPPLPGSPGMPL       EIKASKVEPKRVEP
Sbjct: 61  PPAPSSLPGSAAAQSAAAVAYNSLPPPPPPLPGSPGMPLQQGKSMFEIKASKVEPKRVEP 120

Query: 121 AIQEVDENDFEIECSVGPLRRRRSNRDGSGRGGRAGPGELAEEENGPPPPFPPSSENRRV 180
            I+EVDENDFEIECSVGPLRRRRSNRDG GRGGR GPGELAEEENGPPPP PPSSENRRV
Sbjct: 121 VIEEVDENDFEIECSVGPLRRRRSNRDGGGRGGRTGPGELAEEENGPPPPLPPSSENRRV 180

Query: 181 PVPSPQDSTYDYLFSVDNMPAPTLSGVEDFGANTETVERRAAMEKSGEEPPSSSAGKTSK 240
           P PS QDSTYDYLFSV+NMPAPTLSGVEDF  NTE +ERRAA EKSGEEPPSSSAGKTSK
Sbjct: 181 PAPSAQDSTYDYLFSVENMPAPTLSGVEDFSTNTEAIERRAAAEKSGEEPPSSSAGKTSK 240

Query: 241 KMKQVGYPGSSEGKRIVQGNINLLQIFMDLDDHFLKASESAHDVSKMLEATRLHFHSNFA 300
           KMKQVG+PGSSEGKRIV+GNINLLQIFM+LDDHFLKASESAHDVSKMLEATRLHFHSNFA
Sbjct: 241 KMKQVGFPGSSEGKRIVKGNINLLQIFMELDDHFLKASESAHDVSKMLEATRLHFHSNFA 300

Query: 301 DNRGHIDHSARVMRVITWNRSFRGLPNNDDLNDGFDTEENETHATVLDKLLAWEKKLFEE 360
           DNRGHIDHSARVMRVITWNRSFRGLPNNDDLND FD EENETHATVLDKLLAWEKKLFEE
Sbjct: 301 DNRGHIDHSARVMRVITWNRSFRGLPNNDDLNDDFDREENETHATVLDKLLAWEKKLFEE 360

Query: 361 VKAGEIMKFEYQKKVAALNKLKKKGSNFEAIEKAKATVSHLHTRYIVDMQSMDSTVSEIN 420
           VKAGE+MKFEYQKKVAALNKLKKKGSNFEAIEKAKATVSHLHTRYIVDMQSMDSTVSEIN
Sbjct: 361 VKAGEVMKFEYQKKVAALNKLKKKGSNFEAIEKAKATVSHLHTRYIVDMQSMDSTVSEIN 420

Query: 421 RIRDEQLYPKLVHLIIGMASMWETMHFHHGSQLKVVAALRMLDISQSPKETSDHHHERTV 480
           RIRDEQLYPKLV L+ GMASMWE MHFHHGSQLKVVAALRMLDISQSPKETSDHHHERTV
Sbjct: 421 RIRDEQLYPKLVQLVNGMASMWEIMHFHHGSQLKVVAALRMLDISQSPKETSDHHHERTV 480

Query: 481 QLWAVVQEWHSQLEKLVNRQKDYIKALSNWLRLNLIPTESSLKEKVSSPPRVRSPPIQIL 540
           QLWAVVQEWHSQLEKLVNRQKDYIKALSNWLRLNLIPTESSLKEKVSSPPRVRSPPIQ L
Sbjct: 481 QLWAVVQEWHSQLEKLVNRQKDYIKALSNWLRLNLIPTESSLKEKVSSPPRVRSPPIQSL 540

Query: 541 LHAWQDHLEKLPDEVLRNAIFTFATVIHTIMQSQEEEMKLKLKCQETEKELARKSKQFKD 600
           LH WQDHLEKLPDEVLRN+IFTFATVIHTIMQSQEEEMKLK+KCQETEKELARKSKQFKD
Sbjct: 541 LHVWQDHLEKLPDEVLRNSIFTFATVIHTIMQSQEEEMKLKVKCQETEKELARKSKQFKD 600

Query: 601 WQKKYVQRRGSNADEVDMEEPADKD-AIAERQAAVEAVEKKLEEEREEHQKLCLHVREKS 660
           WQKKY+QRR SNADEV++EE  DKD AIAERQAAVEAVEK+LEEEREEHQKLCLHVREKS
Sbjct: 601 WQKKYMQRRASNADEVNLEENGDKDDAIAERQAAVEAVEKRLEEEREEHQKLCLHVREKS 660

Query: 661 LGSLKNQLPELFRALFEFSLACSRMYRHLKSISQPMPNGPQNQTT 697
           LGSLKNQLPELFRALFEFSLACSRMYRHLKSISQP+ N P +QTT
Sbjct: 661 LGSLKNQLPELFRALFEFSLACSRMYRHLKSISQPLLNRPLSQTT 705

BLAST of Chy1G020640 vs. NCBI nr
Match: XP_022954122.1 (nitrate regulatory gene2 protein-like [Cucurbita moschata] >XP_022954123.1 nitrate regulatory gene2 protein-like [Cucurbita moschata])

HSP 1 Score: 1192 bits (3084), Expect = 0.0
Identity = 629/714 (88.10%), Postives = 654/714 (91.60%), Query Frame = 0

Query: 1   MGCSQSKIENEEVVSRCKDRKMFMKDAVTARNAFAAAHSSYAMSLKNTGAVLSDYAHGEG 60
           MGCSQSKIENEEVVSRCKDRKMFMK+AV ARNAFAAAHSSYAMSLKNTGAVLSDYAHGE 
Sbjct: 1   MGCSQSKIENEEVVSRCKDRKMFMKEAVAARNAFAAAHSSYAMSLKNTGAVLSDYAHGEV 60

Query: 61  PPAPSSLPGSAVVQSAA---AAGYNSLPPPPPPLPGSPGMPL-------EIKASKVEPKR 120
           PPA SSLPG AV QSAA   +A YNSLPPPPPPLPGSPGMPL       EIKASKVEPKR
Sbjct: 61  PPAASSLPGVAVAQSAAVAASASYNSLPPPPPPLPGSPGMPLRNGTSMFEIKASKVEPKR 120

Query: 121 VEPAIQEVDENDFEIECSVGPLRRRRSNRDGSGRGGRAGPGELAEEENGPPPPFPPS--- 180
           VE  I+EVDENDFEIECSVGPLRRR SNR+G GRGGR G GELAEEENGPPPP PPS   
Sbjct: 121 VETVIEEVDENDFEIECSVGPLRRR-SNREGGGRGGRTGLGELAEEENGPPPPLPPSLNR 180

Query: 181 ----SENRRVPVPSPQDSTYDYLFSVDNMPAPTLSGVEDFGANTETVERRAAMEKSGEEP 240
               +ENRR   PSPQD+TYDYLFSV+NMPAPTLS VEDFG NTE +ERRAA+EKSG E 
Sbjct: 181 PPPPNENRRAHSPSPQDATYDYLFSVENMPAPTLSSVEDFGTNTEAIERRAAVEKSGGEL 240

Query: 241 PSSSAGKTSKKMKQVGYPGSSEGKRIVQGNINLLQIFMDLDDHFLKASESAHDVSKMLEA 300
           PSSSAGKTSKK+KQVG+P S EGKR V+GN +LLQIFM+LDDHFLKASESAHDVSKMLEA
Sbjct: 241 PSSSAGKTSKKLKQVGFPCSIEGKRAVKGNTSLLQIFMELDDHFLKASESAHDVSKMLEA 300

Query: 301 TRLHFHSNFADNRGHIDHSARVMRVITWNRSFRGLPNNDDLNDGFDTEENETHATVLDKL 360
           TRLHFHSNFADNRGHIDHSARVMRVITWNRSFRGLPNNDDLND FDTEE+ETHATVLDKL
Sbjct: 301 TRLHFHSNFADNRGHIDHSARVMRVITWNRSFRGLPNNDDLNDDFDTEEHETHATVLDKL 360

Query: 361 LAWEKKLFEEVKAGEIMKFEYQKKVAALNKLKKKGSNFEAIEKAKATVSHLHTRYIVDMQ 420
           LAWEKKLFEEVKAGE+MKFEYQKKVAALNKLKKKGSNFEAIEKAKATVSHLHTRYIVDMQ
Sbjct: 361 LAWEKKLFEEVKAGEVMKFEYQKKVAALNKLKKKGSNFEAIEKAKATVSHLHTRYIVDMQ 420

Query: 421 SMDSTVSEINRIRDEQLYPKLVHLIIGMASMWETMHFHHGSQLKVVAALRMLDISQSPKE 480
           SMDSTVSEINRIRDEQLYPKLV L+ GMASMWE MHFHHG QLK VAALR LDI QSPKE
Sbjct: 421 SMDSTVSEINRIRDEQLYPKLVQLVNGMASMWEIMHFHHGGQLKAVAALRTLDIPQSPKE 480

Query: 481 TSDHHHERTVQLWAVVQEWHSQLEKLVNRQKDYIKALSNWLRLNLIPTESSLKEKVSSPP 540
           TSDHHHERTVQLWAVVQEWHSQLEKLV RQK+YIKALSNWLRLNLIPTESSLKEKVSSPP
Sbjct: 481 TSDHHHERTVQLWAVVQEWHSQLEKLVTRQKEYIKALSNWLRLNLIPTESSLKEKVSSPP 540

Query: 541 RVRSPPIQILLHAWQDHLEKLPDEVLRNAIFTFATVIHTIMQSQEEEMKLKLKCQETEKE 600
           RVRSPPIQ LLH WQDHLEKLPDEVLRNAIFTFATVI+TI+QSQEEEMKLK+KCQETEKE
Sbjct: 541 RVRSPPIQSLLHVWQDHLEKLPDEVLRNAIFTFATVINTIVQSQEEEMKLKVKCQETEKE 600

Query: 601 LARKSKQFKDWQKKYVQRRGSNADEVDMEEPADKDAIAERQAAVEAVEKKLEEEREEHQK 660
           LARKSKQFKDWQKKYVQRR  NADE + EE  DKDAIAERQAAVEAVEK+LEEEREEHQK
Sbjct: 601 LARKSKQFKDWQKKYVQRRAPNADEANQEETGDKDAIAERQAAVEAVEKRLEEEREEHQK 660

Query: 661 LCLHVREKSLGSLKNQLPELFRALFEFSLACSRMYRHLKSISQPMPNGPQNQTT 697
           LCLHVREKSLGSLKNQLPELFRALFEFSLACSRMYRHLKSISQP+PN PQ+QTT
Sbjct: 661 LCLHVREKSLGSLKNQLPELFRALFEFSLACSRMYRHLKSISQPLPNRPQSQTT 713

BLAST of Chy1G020640 vs. TAIR 10
Match: AT1G52320.2 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: N-terminal protein myristoylation; LOCATED IN: plasma membrane; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF630 (InterPro:IPR006868), Protein of unknown function DUF632 (InterPro:IPR006867); BEST Arabidopsis thaliana protein match is: Protein of unknown function (DUF630 and DUF632) (TAIR:AT5G25590.1); Has 8725 Blast hits to 7476 proteins in 620 species: Archae - 10; Bacteria - 622; Metazoa - 3286; Fungi - 1319; Plants - 1442; Viruses - 221; Other Eukaryotes - 1825 (source: NCBI BLink). )

HSP 1 Score: 669.8 bits (1727), Expect = 2.3e-192
Identity = 402/800 (50.25%), Postives = 503/800 (62.88%), Query Frame = 0

Query: 1   MGCSQSKIENEEVVSRCKDRKMFMKDAVTARNAFAAAHSSYAMSLKNTGAVLSDYAHGE- 60
           MGC+QSKIENEE V+RCK+RK  MKDAVTARNAFAAAHS+YAM+LKNTGA LSDY+HGE 
Sbjct: 1   MGCAQSKIENEEAVTRCKERKQLMKDAVTARNAFAAAHSAYAMALKNTGAALSDYSHGEF 60

Query: 61  ------------------------GPPAPSS---LPGSAVVQSAAAAGY---NSLPPPPP 120
                                    PP PSS   +  S    S+AA      ++LPPPPP
Sbjct: 61  LVSNHSSSSAAAAIASTSSLPTAISPPLPSSTAPVSNSTASSSSAAVPQPIPDTLPPPPP 120

Query: 121 PLPGSPGMPLEIKASKVEPK---------------RVEPAI-----QEVDENDFEIECSV 180
           P    P +PL+  A+  E                   + A+      + D++D E+E   
Sbjct: 121 P----PPLPLQRAATMPEMNGRSGGGHAGSGLNGIEEDGALDNDDDDDDDDDDSEMENRD 180

Query: 181 GPLRRRRSNRDGSGRGGRA--GPGELAEEENGPPP------PFPPSSENRRVPVPSPQDS 240
             +R+ RS R GS RG R       L EE+  PPP      P PP  +++       Q  
Sbjct: 181 RLIRKSRS-RGGSTRGNRTTIEDHHLQEEKAPPPPPLANSRPIPPPRQHQHQHQQQQQQP 240

Query: 241 TYDYLF-SVDNMPAPTLSGV--------------------------------EDFGANTE 300
            YDY F +V+NMP  TL                                   E+     E
Sbjct: 241 FYDYFFPNVENMPGTTLEDTPPQPQPQPTRPVPPQPHSPVVTEDDEDEEEEEEEEEEEEE 300

Query: 301 TV--------ERRAAMEKSGEEPPSSSAGKTSKKMKQVGYPGSSEGKRIVQGNINLLQIF 360
           TV        ER   +E+   E    +  +  KK K +G PG   G R+     +L  +F
Sbjct: 301 TVIERKPLVEERPKRVEEVTIELEKVTNLRGMKKSKGIGIPGERRGMRMPVTATHLANVF 360

Query: 361 MDLDDHFLKASESAHDVSKMLEATRLHFHSNFADNRGHIDHSARVMRVITWNRSFRGLPN 420
           ++LDD+FLKASESAHDVSKMLEATRLH+HSNFADNRGHIDHSARVMRVITWNRSFRG+PN
Sbjct: 361 IELDDNFLKASESAHDVSKMLEATRLHYHSNFADNRGHIDHSARVMRVITWNRSFRGIPN 420

Query: 421 NDDLNDGFDTEENETHATVLDKLLAWEKKLFEEVKAGEIMKFEYQKKVAALNKLKKKGSN 480
            DD  D  D EENETHATVLDKLLAWEKKL++EVKAGE+MK EYQKKVA LN++KK+G +
Sbjct: 421 ADDGKDDVDLEENETHATVLDKLLAWEKKLYDEVKAGELMKIEYQKKVAHLNRVKKRGGH 480

Query: 481 FEAIEKAKATVSHLHTRYIVDMQSMDSTVSEINRIRDEQLYPKLVHLIIGMASMWETMHF 540
            +++E+AKA VSHLHTRYIVDMQSMDSTVSEINR+RDEQLY KLVHL+  M  MWE M  
Sbjct: 481 SDSLERAKAAVSHLHTRYIVDMQSMDSTVSEINRLRDEQLYLKLVHLVEAMGKMWEMMQI 540

Query: 541 HHGSQLKVVAALRMLDISQSPKETSDHHHERTVQLWAVVQEWHSQLEKLVNRQKDYIKAL 600
           HH  Q ++   LR LD+SQ+ KET+DHHHERT+QL AVVQEWH+Q  ++++ QK+YIKAL
Sbjct: 541 HHQRQAEISKVLRSLDVSQAVKETNDHHHERTIQLLAVVQEWHTQFCRMIDHQKEYIKAL 600

Query: 601 SNWLRLNLIPTESSLKEKVSSPPRVRSPPIQILLHAWQDHLEKLPDEVLRNAIFTFATVI 660
             WL+LNLIP ES+LKEKVSSPPRV +P IQ LLHAW D L+K+PDE+ ++AI  FA V+
Sbjct: 601 GGWLKLNLIPIESTLKEKVSSPPRVPNPAIQKLLHAWYDRLDKIPDEMAKSAIINFAAVV 660

Query: 661 HTIMQSQEEEMKLKLKCQETEKELARKSKQFKDWQKKYVQRRGSNADEVDMEEPADKDAI 701
            TIMQ QE+E+ L+ KC+ET KEL RK +QF+DW  KY+Q+RG      D  +    D +
Sbjct: 661 STIMQQQEDEISLRNKCEETRKELGRKIRQFEDWYHKYIQKRGPEGMNPDEADNDHNDEV 720

BLAST of Chy1G020640 vs. TAIR 10
Match: AT5G25590.1 (Protein of unknown function (DUF630 and DUF632) )

HSP 1 Score: 595.5 bits (1534), Expect = 5.5e-170
Identity = 363/790 (45.95%), Postives = 480/790 (60.76%), Query Frame = 0

Query: 1   MGCSQSKIENEEVVSRCKDRKMFMKDAVTARNAFAAAHSSYAMSLKNTGAVLSDYAHGEG 60
           MGC+QS+++NEE V+RCK+R+  +K+AV+A  AFAA H +YA++LKNTGA LSDY HGE 
Sbjct: 1   MGCAQSRVDNEEAVARCKERRNVIKEAVSASKAFAAGHFAYAIALKNTGAALSDYGHGES 60

Query: 61  ------------------------PPAPSSLPGSAVVQSAAAAGYNSLPPPPPPLPGSPG 120
                                   P +P   P   +          +LPPPPPPLP    
Sbjct: 61  DQKALDDVLLDQQHYEKQSRNNVDPASPQPPPPPPI---------ENLPPPPPPLPKFSP 120

Query: 121 MPLE----IKASKVEPKRVEP----AIQEVDENDFEIECSVGPLRRRRSNRDGSGRGGRA 180
            P++    + +  V  ++V+     AI+E +E++ E E              GSGR    
Sbjct: 121 SPIKRAISLPSMAVRGRKVQTLDGMAIEEEEEDEEEEE-----------EVKGSGR---- 180

Query: 181 GPGELAEEENGPPPPFPPSSENRR---------VPVPSPQDS-TYDYLFSVDNMPAPTL- 240
              + A+EE  P  P      N R         +   SP +S  +DY F V+NMP P L 
Sbjct: 181 ---DTAQEEEEPRTPENVGKSNGRKRLEKTTPEIVSASPANSMAWDYFFMVENMPGPNLD 240

Query: 241 -----SGVED----FGANTETVERRAAMEKSGEEPPSSSAGKTSKKMKQ----------- 300
                +G E+    F  N E  E     E+SG     S +GK  ++M+            
Sbjct: 241 DREVRNGYENQSSHFQFNEEDDEEEEEEERSGIYRKKSGSGKVVEEMEPKTPEKVEEEEE 300

Query: 301 ------------------VGYPGSSEGKRIVQ---------------------GNINLLQ 360
                             V      +GK  ++                      ++NL++
Sbjct: 301 EDEEEDEEEEEEEEEEVVVEVKKKKKGKAKIEHSSTAPPEFRRAVAKTSAAASSSVNLMK 360

Query: 361 IFMDLDDHFLKASESAHDVSKMLEATRLHFHSNFADNRGHIDHSARVMRVITWNRSFRGL 420
           I  ++DD FLKASE A +VSKMLEATRLH+HSNFADNRG++DHSARVMRVITWN+S RG+
Sbjct: 361 ILDEIDDRFLKASECAQEVSKMLEATRLHYHSNFADNRGYVDHSARVMRVITWNKSLRGI 420

Query: 421 PNNDDLNDGFDTEENETHATVLDKLLAWEKKLFEEVKAGEIMKFEYQKKVAALNKLKKKG 480
            N +   D  +++E+ETHATVLDKLLAWEKKL++EVK GE+MK EYQKKV+ LN+ KK+G
Sbjct: 421 SNGEGGKDDQESDEHETHATVLDKLLAWEKKLYDEVKQGELMKIEYQKKVSLLNRHKKRG 480

Query: 481 SNFEAIEKAKATVSHLHTRYIVDMQSMDSTVSEINRIRDEQLYPKLVHLIIGMASMWETM 540
           ++ E +EK KA VSHLHTRYIVDMQSMDSTVSE+NR+RD+QLYP+LV L+ GMA MW  M
Sbjct: 481 ASAETVEKTKAAVSHLHTRYIVDMQSMDSTVSEVNRLRDDQLYPRLVALVEGMAKMWTNM 540

Query: 541 HFHHGSQLKVVAALRMLDISQSPKETSDHHHERTVQLWAVVQEWHSQLEKLVNRQKDYIK 600
             HH +QL +V  L+ L+IS S KET+  HH +T Q   V++EWH Q + LV  QK YI 
Sbjct: 541 CIHHDTQLGIVGELKALEISTSLKETTKQHHHQTRQFCTVLEEWHVQFDTLVTHQKQYIN 600

Query: 601 ALSNWLRLNLIPTESSLKEKVSSPPRVRSPPIQILLHAWQDHLEKLPDEVLRNAIFTFAT 660
           +L+NWL+LNLIP ESSLKEKVSSPPR + PPIQ LLH+W D LEKLPDEV ++AI +FA 
Sbjct: 601 SLNNWLKLNLIPIESSLKEKVSSPPRPQRPPIQALLHSWHDRLEKLPDEVAKSAISSFAA 660

Query: 661 VIHTIMQSQEEEMKLKLKCQETEKELARKSKQFKDWQKKYVQRRG--SNADEVDMEEPAD 687
           VI TI+  QEEEMKLK KC+ET +E  RK + F+DW +K++Q+RG    A+  D    + 
Sbjct: 661 VIKTILLHQEEEMKLKEKCEETRREFIRKKQGFEDWYQKHLQKRGPTEEAEGGDDATTSS 720

BLAST of Chy1G020640 vs. TAIR 10
Match: AT1G52320.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: N-terminal protein myristoylation; LOCATED IN: plasma membrane; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF632 (InterPro:IPR006867); BEST Arabidopsis thaliana protein match is: Protein of unknown function (DUF630 and DUF632) (TAIR:AT5G25590.1); Has 517 Blast hits to 513 proteins in 62 species: Archae - 6; Bacteria - 6; Metazoa - 50; Fungi - 2; Plants - 427; Viruses - 0; Other Eukaryotes - 26 (source: NCBI BLink). )

HSP 1 Score: 574.7 bits (1480), Expect = 1.0e-163
Identity = 290/468 (61.97%), Postives = 359/468 (76.71%), Query Frame = 0

Query: 233 KKMKQVGYPGSSEGKRIVQGNINLLQIFMDLDDHFLKASESAHDVSKMLEATRLHFHSNF 292
           KK K +G PG   G R+     +L  +F++LDD+FLKASESAHDVSKMLEATRLH+HSNF
Sbjct: 2   KKSKGIGIPGERRGMRMPVTATHLANVFIELDDNFLKASESAHDVSKMLEATRLHYHSNF 61

Query: 293 ADNRGHIDHSARVMRVITWNRSFRGLPNNDDLNDGFDTEENETHATVLDKLLAWEKKLFE 352
           ADNRGHIDHSARVMRVITWNRSFRG+PN DD  D  D EENETHATVLDKLLAWEKKL++
Sbjct: 62  ADNRGHIDHSARVMRVITWNRSFRGIPNADDGKDDVDLEENETHATVLDKLLAWEKKLYD 121

Query: 353 EVKAGEIMKFEYQKKVAALNKLKKKGSNFEAIEKAKATVSHLHTRYIVDMQSMDSTVSEI 412
           EVKAGE+MK EYQKKVA LN++KK+G + +++E+AKA VSHLHTRYIVDMQSMDSTVSEI
Sbjct: 122 EVKAGELMKIEYQKKVAHLNRVKKRGGHSDSLERAKAAVSHLHTRYIVDMQSMDSTVSEI 181

Query: 413 NRIRDEQLYPKLVHLIIGMASMWETMHFHHGSQLKVVAALRMLDISQSPKETSDHHHERT 472
           NR+RDEQLY KLVHL+  M  MWE M  HH  Q ++   LR LD+SQ+ KET+DHHHERT
Sbjct: 182 NRLRDEQLYLKLVHLVEAMGKMWEMMQIHHQRQAEISKVLRSLDVSQAVKETNDHHHERT 241

Query: 473 VQLWAVVQEWHSQLEKLVNRQKDYIKALSNWLRLNLIPTESSLKEKVSSPPRVRSPPIQI 532
           +QL AVVQEWH+Q  ++++ QK+YIKAL  WL+LNLIP ES+LKEKVSSPPRV +P IQ 
Sbjct: 242 IQLLAVVQEWHTQFCRMIDHQKEYIKALGGWLKLNLIPIESTLKEKVSSPPRVPNPAIQK 301

Query: 533 LLHAWQDHLEKLPDEVLRNAIFTFATVIHTIMQSQEEEMKLKLKCQETEKELARKSKQFK 592
           LLHAW D L+K+PDE+ ++AI  FA V+ TIMQ QE+E+ L+ KC+ET KEL RK +QF+
Sbjct: 302 LLHAWYDRLDKIPDEMAKSAIINFAAVVSTIMQQQEDEISLRNKCEETRKELGRKIRQFE 361

Query: 593 DWQKKYVQRRGSNADEVDMEEPADKDAIAERQAAVEAVEKKLEEEREEHQKLCLHVREKS 652
           DW  KY+Q+RG      D  +    D +A RQ  VE ++K+LEEE E + +    VREKS
Sbjct: 362 DWYHKYIQKRGPEGMNPDEADNDHNDEVAVRQFNVEQIKKRLEEEEEAYHRQSHQVREKS 421

Query: 653 LGSLKNQLPELFRALFEFSLACSRMYRHLKSISQPMPNGPQNQTTTLG 701
           L SL+ +LPELF+A+ E + +CS MYR +   S+      ++Q  + G
Sbjct: 422 LASLRTRLPELFQAMSEVAYSCSDMYRAITYASKRQSQSERHQKPSQG 469

BLAST of Chy1G020640 vs. TAIR 10
Match: AT1G52320.3 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: N-terminal protein myristoylation; LOCATED IN: plasma membrane; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF632 (InterPro:IPR006867); BEST Arabidopsis thaliana protein match is: Protein of unknown function (DUF630 and DUF632) (TAIR:AT5G25590.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 574.7 bits (1480), Expect = 1.0e-163
Identity = 290/468 (61.97%), Postives = 359/468 (76.71%), Query Frame = 0

Query: 233 KKMKQVGYPGSSEGKRIVQGNINLLQIFMDLDDHFLKASESAHDVSKMLEATRLHFHSNF 292
           KK K +G PG   G R+     +L  +F++LDD+FLKASESAHDVSKMLEATRLH+HSNF
Sbjct: 2   KKSKGIGIPGERRGMRMPVTATHLANVFIELDDNFLKASESAHDVSKMLEATRLHYHSNF 61

Query: 293 ADNRGHIDHSARVMRVITWNRSFRGLPNNDDLNDGFDTEENETHATVLDKLLAWEKKLFE 352
           ADNRGHIDHSARVMRVITWNRSFRG+PN DD  D  D EENETHATVLDKLLAWEKKL++
Sbjct: 62  ADNRGHIDHSARVMRVITWNRSFRGIPNADDGKDDVDLEENETHATVLDKLLAWEKKLYD 121

Query: 353 EVKAGEIMKFEYQKKVAALNKLKKKGSNFEAIEKAKATVSHLHTRYIVDMQSMDSTVSEI 412
           EVKAGE+MK EYQKKVA LN++KK+G + +++E+AKA VSHLHTRYIVDMQSMDSTVSEI
Sbjct: 122 EVKAGELMKIEYQKKVAHLNRVKKRGGHSDSLERAKAAVSHLHTRYIVDMQSMDSTVSEI 181

Query: 413 NRIRDEQLYPKLVHLIIGMASMWETMHFHHGSQLKVVAALRMLDISQSPKETSDHHHERT 472
           NR+RDEQLY KLVHL+  M  MWE M  HH  Q ++   LR LD+SQ+ KET+DHHHERT
Sbjct: 182 NRLRDEQLYLKLVHLVEAMGKMWEMMQIHHQRQAEISKVLRSLDVSQAVKETNDHHHERT 241

Query: 473 VQLWAVVQEWHSQLEKLVNRQKDYIKALSNWLRLNLIPTESSLKEKVSSPPRVRSPPIQI 532
           +QL AVVQEWH+Q  ++++ QK+YIKAL  WL+LNLIP ES+LKEKVSSPPRV +P IQ 
Sbjct: 242 IQLLAVVQEWHTQFCRMIDHQKEYIKALGGWLKLNLIPIESTLKEKVSSPPRVPNPAIQK 301

Query: 533 LLHAWQDHLEKLPDEVLRNAIFTFATVIHTIMQSQEEEMKLKLKCQETEKELARKSKQFK 592
           LLHAW D L+K+PDE+ ++AI  FA V+ TIMQ QE+E+ L+ KC+ET KEL RK +QF+
Sbjct: 302 LLHAWYDRLDKIPDEMAKSAIINFAAVVSTIMQQQEDEISLRNKCEETRKELGRKIRQFE 361

Query: 593 DWQKKYVQRRGSNADEVDMEEPADKDAIAERQAAVEAVEKKLEEEREEHQKLCLHVREKS 652
           DW  KY+Q+RG      D  +    D +A RQ  VE ++K+LEEE E + +    VREKS
Sbjct: 362 DWYHKYIQKRGPEGMNPDEADNDHNDEVAVRQFNVEQIKKRLEEEEEAYHRQSHQVREKS 421

Query: 653 LGSLKNQLPELFRALFEFSLACSRMYRHLKSISQPMPNGPQNQTTTLG 701
           L SL+ +LPELF+A+ E + +CS MYR +   S+      ++Q  + G
Sbjct: 422 LASLRTRLPELFQAMSEVAYSCSDMYRAITYASKRQSQSERHQKPSQG 469

BLAST of Chy1G020640 vs. TAIR 10
Match: AT1G52320.4 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: N-terminal protein myristoylation; LOCATED IN: plasma membrane; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF632 (InterPro:IPR006867); BEST Arabidopsis thaliana protein match is: Protein of unknown function (DUF630 and DUF632) (TAIR:AT5G25590.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 574.7 bits (1480), Expect = 1.0e-163
Identity = 290/468 (61.97%), Postives = 359/468 (76.71%), Query Frame = 0

Query: 233 KKMKQVGYPGSSEGKRIVQGNINLLQIFMDLDDHFLKASESAHDVSKMLEATRLHFHSNF 292
           KK K +G PG   G R+     +L  +F++LDD+FLKASESAHDVSKMLEATRLH+HSNF
Sbjct: 2   KKSKGIGIPGERRGMRMPVTATHLANVFIELDDNFLKASESAHDVSKMLEATRLHYHSNF 61

Query: 293 ADNRGHIDHSARVMRVITWNRSFRGLPNNDDLNDGFDTEENETHATVLDKLLAWEKKLFE 352
           ADNRGHIDHSARVMRVITWNRSFRG+PN DD  D  D EENETHATVLDKLLAWEKKL++
Sbjct: 62  ADNRGHIDHSARVMRVITWNRSFRGIPNADDGKDDVDLEENETHATVLDKLLAWEKKLYD 121

Query: 353 EVKAGEIMKFEYQKKVAALNKLKKKGSNFEAIEKAKATVSHLHTRYIVDMQSMDSTVSEI 412
           EVKAGE+MK EYQKKVA LN++KK+G + +++E+AKA VSHLHTRYIVDMQSMDSTVSEI
Sbjct: 122 EVKAGELMKIEYQKKVAHLNRVKKRGGHSDSLERAKAAVSHLHTRYIVDMQSMDSTVSEI 181

Query: 413 NRIRDEQLYPKLVHLIIGMASMWETMHFHHGSQLKVVAALRMLDISQSPKETSDHHHERT 472
           NR+RDEQLY KLVHL+  M  MWE M  HH  Q ++   LR LD+SQ+ KET+DHHHERT
Sbjct: 182 NRLRDEQLYLKLVHLVEAMGKMWEMMQIHHQRQAEISKVLRSLDVSQAVKETNDHHHERT 241

Query: 473 VQLWAVVQEWHSQLEKLVNRQKDYIKALSNWLRLNLIPTESSLKEKVSSPPRVRSPPIQI 532
           +QL AVVQEWH+Q  ++++ QK+YIKAL  WL+LNLIP ES+LKEKVSSPPRV +P IQ 
Sbjct: 242 IQLLAVVQEWHTQFCRMIDHQKEYIKALGGWLKLNLIPIESTLKEKVSSPPRVPNPAIQK 301

Query: 533 LLHAWQDHLEKLPDEVLRNAIFTFATVIHTIMQSQEEEMKLKLKCQETEKELARKSKQFK 592
           LLHAW D L+K+PDE+ ++AI  FA V+ TIMQ QE+E+ L+ KC+ET KEL RK +QF+
Sbjct: 302 LLHAWYDRLDKIPDEMAKSAIINFAAVVSTIMQQQEDEISLRNKCEETRKELGRKIRQFE 361

Query: 593 DWQKKYVQRRGSNADEVDMEEPADKDAIAERQAAVEAVEKKLEEEREEHQKLCLHVREKS 652
           DW  KY+Q+RG      D  +    D +A RQ  VE ++K+LEEE E + +    VREKS
Sbjct: 362 DWYHKYIQKRGPEGMNPDEADNDHNDEVAVRQFNVEQIKKRLEEEEEAYHRQSHQVREKS 421

Query: 653 LGSLKNQLPELFRALFEFSLACSRMYRHLKSISQPMPNGPQNQTTTLG 701
           L SL+ +LPELF+A+ E + +CS MYR +   S+      ++Q  + G
Sbjct: 422 LASLRTRLPELFQAMSEVAYSCSDMYRAITYASKRQSQSERHQKPSQG 469

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9AQW13.4e-6328.74Protein ROLLING AND ERECT LEAF 2 OS=Oryza sativa subsp. japonica OX=39947 GN=REL... [more]
A0A178VBJ01.1e-6128.31Protein ALTERED PHOSPHATE STARVATION RESPONSE 1 OS=Arabidopsis thaliana OX=3702 ... [more]
Q93YU82.6e-4725.16Nitrate regulatory gene2 protein OS=Arabidopsis thaliana OX=3702 GN=NRG2 PE=1 SV... [more]
Match NameE-valueIdentityDescription
A0A0A0K7A30.0e+0098.71Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G446740 PE=4 SV=1[more]
A0A1S4E3Q80.0e+0097.14uncharacterized protein LOC103500575 OS=Cucumis melo OX=3656 GN=LOC103500575 PE=... [more]
A0A5A7UW130.0e+0097.00Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold... [more]
A0A6J1GQ840.0e+0088.10nitrate regulatory gene2 protein-like OS=Cucurbita moschata OX=3662 GN=LOC111456... [more]
A0A6J1JYE90.0e+0087.82nitrate regulatory gene2 protein-like OS=Cucurbita maxima OX=3661 GN=LOC11148857... [more]
Match NameE-valueIdentityDescription
XP_004141776.10.098.71nitrate regulatory gene2 protein [Cucumis sativus] >XP_011659565.1 nitrate regul... [more]
XP_008462152.10.097.14PREDICTED: uncharacterized protein LOC103500575 [Cucumis melo] >XP_016902857.1 P... [more]
KAA0059290.10.097.00uncharacterized protein E6C27_scaffold242G00130 [Cucumis melo var. makuwa][more]
XP_038899337.10.092.62protein ROLLING AND ERECT LEAF 2-like [Benincasa hispida][more]
XP_022954122.10.088.10nitrate regulatory gene2 protein-like [Cucurbita moschata] >XP_022954123.1 nitra... [more]
Match NameE-valueIdentityDescription
AT1G52320.22.3e-19250.25unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: N-termin... [more]
AT5G25590.15.5e-17045.95Protein of unknown function (DUF630 and DUF632) [more]
AT1G52320.11.0e-16361.97unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: N-termin... [more]
AT1G52320.31.0e-16361.97unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: N-termin... [more]
AT1G52320.41.0e-16361.97unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: N-termin... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 563..597
NoneNo IPR availableCOILSCoilCoilcoord: 620..651
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 133..186
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 211..246
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 82..96
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 156..172
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 78..100
NoneNo IPR availablePANTHERPTHR21450:SF33PROTEIN, PUTATIVE, 48652-45869-RELATEDcoord: 1..688
NoneNo IPR availablePANTHERPTHR21450UNCHARACTERIZEDcoord: 1..688
IPR006867Domain of unknown function DUF632PFAMPF04782DUF632coord: 256..561
e-value: 9.3E-102
score: 340.6
IPR006868Domain of unknown function DUF630PFAMPF04783DUF630coord: 1..59
e-value: 5.0E-24
score: 84.3

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy1G020640.1Chy1G020640.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016020 membrane