Chy1G020340 (gene) Cucumber (hystrix) v1

Overview


Name	Chy1G020340
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	gibberellin 3-beta-dioxygenase 1-like
Location	chrH01: 25185876 .. 25187640 (-)
RNA-Seq Expression	Chy1G020340
Synteny	Chy1G020340

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGCGACGTCTTCAAAACTCATGACCCTCTCAACATTCCCACCAACAAAAACCTCGACTCTGATTCCGACTCTATCCCTGTAATTGACCTTTCTCTTCCTAATGCTCCCGCGCTCATGAACAATGCCTTTCAAACTTGGGGGGTATGCCATGTCCTTAACCACGGCGTTCCCCTTTCCCTACTCAAATCCATGGAGTCCTTCATCAATGATCTCTTCGATCTTCCCACTTCACAGAAGCTTAAGGTCACTCGTACCCCCGAATCCATCGCCGGCTTCGGACTCGTTCCTCTCTCCAAAATATACCCTAAGCGTCCGTGGGGTGAGGGCTTCACCATCATCGGTAATCCTGTCGACCATTTTCAAAAACTCTGGCCTCAAGACTGCAAAAAATACTGGTATTGCTATTACTATCATGGCCAAATATTATGATAATGTGGATAAGTCCTAAAATATTGTTTTCTATATATATATATATATATCTTCTTTTTATAATATCTTTTTTAAAAAAAGGTGCCATTTTTTAATTTAGAATTTGGATAAAAATTCTTAGTGCATGTTAATTATGGTAATAAATTCAAAAGAAAATAGATTTGATTTTCAAGATGTAAAAACAGAAAACAATGTTTCCAAATTGAGCTATAATAATCCAACTATATATAATGAATAGAGCAGGGTCACATATACAATAATAAAATAAGGGAAAGAAATATTGATTCTACATTTTTCACATTGTATTCATTTGAATCATAAAGCTTTCAATTCATCCGTTTTGATTTAATTTGAGCCTAATTCATCCACTATGATCATGATAAAAATTCAGTTTCGTACACAAAGAAATTTGAAGTTGGCACAAATTAAATGATTGAAACTATTCTCAAATTTTCGTAAATTTTAACCAAAAGATTTAATTTGATCACCAAATTTATTTTAAATAATTAAACTTTTGATAATATCTAGAAAGTAATAAAATATCATAATTATTATGATCATATTTTGTTATTTTTTGAAAATAGTTTAGTTCATTTTTTATATTTGAAAATAACTTTTATAAGAAAAAATTATAATTGGAAAACTTGTTTAATTGTGGAACAAATTCTTAAAAAATTGATGATTGGATTGTATAACTTGTAGTGATTTTGTGGAAGAATACAACAAAGAGATGAAGAGTCTATGTGGAAAGCTGTTGTGGCTTACATTGGGAGAATTAGGCATAACTCCAGAGGATATACATTGGGCCGGACCGGATGGGGATTTCAAGACCGATAATCAAGCCATACGATTGAACTCTTACCCGGTCTGCCCAGAACCGGACGACCTAATCGGGCTCCCGCCTCACTCCGACACCAGCGCCTTAACCATTCTGTACCAAACAACAAAAGGGTTACAAGTTTCAATAGAAGGGAAAGGGTGGGTGGATGTGGAGCCGGTAAACGGTGCACTGGTGGTTCAAGTCGGAGACATGCTTCATATTCTTACAAACGGGATGTACTCTCCTTCGGTCCATCAGGCCGTCGTGAACCAGACCGATGATCGTATCTCGACGGCGTACTTCTTTGGACCGCCGCCAAAGGGCGAAGTTTCACCGCTTAAGAAACTTGTGACCCCAACTCAACCATTACGTTACTCCACGGTAACGTGGGCTGACTACCTCCGTAAAAAATATCTTCTTTTTGAGGAAACCCTTCCATCTATCCGTCTTTCCGCTCCAGCTCCTACTGGTCTGTCAATTGGGAACGATCAGAACTTGGTGAAAGTAGGCTAA

mRNA sequence

ATGAGCGACGTCTTCAAAACTCATGACCCTCTCAACATTCCCACCAACAAAAACCTCGACTCTGATTCCGACTCTATCCCTGTAATTGACCTTTCTCTTCCTAATGCTCCCGCGCTCATGAACAATGCCTTTCAAACTTGGGGGGTATGCCATGTCCTTAACCACGGCGTTCCCCTTTCCCTACTCAAATCCATGGAGTCCTTCATCAATGATCTCTTCGATCTTCCCACTTCACAGAAGCTTAAGGTCACTCGTACCCCCGAATCCATCGCCGGCTTCGGACTCGTTCCTCTCTCCAAAATATACCCTAAGCGTCCGTGGGGTGAGGGCTTCACCATCATCGGTAATCCTGTCGACCATTTTCAAAAACTCTGGCCTCAAGACTGCAAAAAATACTGTGATTTTGTGGAAGAATACAACAAAGAGATGAAGAGTCTATGTGGAAAGCTGTTGTGGCTTACATTGGGAGAATTAGGCATAACTCCAGAGGATATACATTGGGCCGGACCGGATGGGGATTTCAAGACCGATAATCAAGCCATACGATTGAACTCTTACCCGGTCTGCCCAGAACCGGACGACCTAATCGGGCTCCCGCCTCACTCCGACACCAGCGCCTTAACCATTCTGTACCAAACAACAAAAGGGTTACAAGTTTCAATAGAAGGGAAAGGGTGGGTGGATGTGGAGCCGGTAAACGGTGCACTGGTGGTTCAAGTCGGAGACATGCTTCATATTCTTACAAACGGGATGTACTCTCCTTCGGTCCATCAGGCCGTCGTGAACCAGACCGATGATCGTATCTCGACGGCGTACTTCTTTGGACCGCCGCCAAAGGGCGAAGTTTCACCGCTTAAGAAACTTGTGACCCCAACTCAACCATTACGTTACTCCACGGTAACGTGGGCTGACTACCTCCGTAAAAAATATCTTCTTTTTGAGGAAACCCTTCCATCTATCCGTCTTTCCGCTCCAGCTCCTACTGGTCTGTCAATTGGGAACGATCAGAACTTGGTGAAAGTAGGCTAA

Coding sequence (CDS)

ATGAGCGACGTCTTCAAAACTCATGACCCTCTCAACATTCCCACCAACAAAAACCTCGACTCTGATTCCGACTCTATCCCTGTAATTGACCTTTCTCTTCCTAATGCTCCCGCGCTCATGAACAATGCCTTTCAAACTTGGGGGGTATGCCATGTCCTTAACCACGGCGTTCCCCTTTCCCTACTCAAATCCATGGAGTCCTTCATCAATGATCTCTTCGATCTTCCCACTTCACAGAAGCTTAAGGTCACTCGTACCCCCGAATCCATCGCCGGCTTCGGACTCGTTCCTCTCTCCAAAATATACCCTAAGCGTCCGTGGGGTGAGGGCTTCACCATCATCGGTAATCCTGTCGACCATTTTCAAAAACTCTGGCCTCAAGACTGCAAAAAATACTGTGATTTTGTGGAAGAATACAACAAAGAGATGAAGAGTCTATGTGGAAAGCTGTTGTGGCTTACATTGGGAGAATTAGGCATAACTCCAGAGGATATACATTGGGCCGGACCGGATGGGGATTTCAAGACCGATAATCAAGCCATACGATTGAACTCTTACCCGGTCTGCCCAGAACCGGACGACCTAATCGGGCTCCCGCCTCACTCCGACACCAGCGCCTTAACCATTCTGTACCAAACAACAAAAGGGTTACAAGTTTCAATAGAAGGGAAAGGGTGGGTGGATGTGGAGCCGGTAAACGGTGCACTGGTGGTTCAAGTCGGAGACATGCTTCATATTCTTACAAACGGGATGTACTCTCCTTCGGTCCATCAGGCCGTCGTGAACCAGACCGATGATCGTATCTCGACGGCGTACTTCTTTGGACCGCCGCCAAAGGGCGAAGTTTCACCGCTTAAGAAACTTGTGACCCCAACTCAACCATTACGTTACTCCACGGTAACGTGGGCTGACTACCTCCGTAAAAAATATCTTCTTTTTGAGGAAACCCTTCCATCTATCCGTCTTTCCGCTCCAGCTCCTACTGGTCTGTCAATTGGGAACGATCAGAACTTGGTGAAAGTAGGCTAA

Protein sequence

MSDVFKTHDPLNIPTNKNLDSDSDSIPVIDLSLPNAPALMNNAFQTWGVCHVLNHGVPLSLLKSMESFINDLFDLPTSQKLKVTRTPESIAGFGLVPLSKIYPKRPWGEGFTIIGNPVDHFQKLWPQDCKKYCDFVEEYNKEMKSLCGKLLWLTLGELGITPEDIHWAGPDGDFKTDNQAIRLNSYPVCPEPDDLIGLPPHSDTSALTILYQTTKGLQVSIEGKGWVDVEPVNGALVVQVGDMLHILTNGMYSPSVHQAVVNQTDDRISTAYFFGPPPKGEVSPLKKLVTPTQPLRYSTVTWADYLRKKYLLFEETLPSIRLSAPAPTGLSIGNDQNLVKVG*

Homology

[Close]

BLAST of Chy1G020340 vs. ExPASy Swiss-Prot
Match: O24648 (Gibberellin 3-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=LE PE=1 SV=1)

HSP 1 Score: 335.9 bits (860), Expect = 5.4e-91
Identity = 160/333 (48.05%), Postives = 221/333 (66.37%), Query Frame = 0


Query: 13  IPTNKNLDSDSDSIPVIDLSLPNAPALMNNAFQTWGVCHVLNHGVPLSLLKSMESFINDL 72
           I +N  +   + ++PVIDL+ PNA  L+  A +TWGV  V+NHG+PLSLL+ ++     L
Sbjct: 43  IDSNNIMKESTTTVPVIDLNDPNASKLIGLACKTWGVYQVMNHGIPLSLLEDIQWLGQTL 102

Query: 73  FDLPTSQKLKVTRTPESIAGFGLVPLSKIYPKRPWGEGFTIIGNPVDHFQKLWPQDCKKY 132
           F LP+ QK K TR+P+ ++G+G+  +S  +PK  W EGFTI+G+P+DHF++LWPQD  ++
Sbjct: 103 FSLPSHQKHKATRSPDGVSGYGIARISSFFPKLMWYEGFTIVGSPLDHFRELWPQDYTRF 162

Query: 133 CDFVEEYNKEMKSLCGKLLWLTLGELGITPEDIHWAGPDGDFKTDNQAIRLNSYPVCPEP 192
           CD V +Y++ MK L G L+ L L  LGIT EDI WAG    F+    A++LNSYP CP+P
Sbjct: 163 CDIVVQYDETMKKLAGTLMCLMLDSLGITKEDIKWAGSKAQFEKACAALQLNSYPSCPDP 222

Query: 193 DDLIGLPPHSDTSALTILYQT-TKGLQVSIEGKGWVDVEPVNGALVVQVGDMLHILTNGM 252
           D  +GL PH+D++ LTIL Q    GLQV+ EG GW+ V P+ G LVV VGD+ HIL+NG+
Sbjct: 223 DHAMGLAPHTDSTFLTILSQNDISGLQVNREGSGWITVPPLQGGLVVNVGDLFHILSNGL 282

Query: 253 YSPSVHQAVVNQTDDRISTAYFFGPPPKGEVSPLKKLVTPTQPLRYSTVTWADYLRKKYL 312
           Y   +H+ +VN+T  R S AY +GPP   E+ P  KL+ PT+P  Y +VTW +YL  K  
Sbjct: 283 YPSVLHRVLVNRTRQRFSVAYLYGPPSNVEICPHAKLIGPTKPPLYRSVTWNEYLGTKAK 342

Query: 313 LFEETLPSIRLSAPAPTGLSIGND--QNLVKVG 343
            F + L S+RL  P   GL   ND  +N V+VG
Sbjct: 343 HFNKALSSVRLCTPI-NGLFDVNDSNKNSVQVG 374

[Close]

BLAST of Chy1G020340 vs. ExPASy Swiss-Prot
Match: Q39103 (Gibberellin 3-beta-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=GA3OX1 PE=1 SV=2)

HSP 1 Score: 302.0 bits (772), Expect = 8.7e-81
Identity = 140/299 (46.82%), Postives = 193/299 (64.55%), Query Frame = 0


Query: 24  DSIPVIDLSLPNAPALMNNAFQTWGVCHVLNHGVPLSLLKSMESFINDLFDLPTSQKLKV 83
           ++IP+IDL  P+A   + +A +TWG   + NHGVPL LL+ +E     LF LP  +KLK 
Sbjct: 55  ENIPLIDLDHPDATNQIGHACRTWGAFQISNHGVPLGLLQDIEFLTGSLFGLPVQRKLKS 114

Query: 84  TRTPESIAGFGLVPLSKIYPKRPWGEGFTIIGNPVDHFQKLWPQDCKKYCDFVEEYNKEM 143
            R+   ++G+G+  ++  + K+ W EGFTI G+P++ F+KLWPQ    YCD VEEY + M
Sbjct: 115 ARSETGVSGYGVARIASFFNKQMWSEGFTITGSPLNDFRKLWPQHHLNYCDIVEEYEEHM 174

Query: 144 KSLCGKLLWLTLGELGITPEDIHWAGPDGDFKTDNQAIRLNSYPVCPEPDDLIGLPPHSD 203
           K L  KL+WL L  LG++ EDI WA    D      A++LN YPVCPEPD  +GL  H+D
Sbjct: 175 KKLASKLMWLALNSLGVSEEDIEWASLSSDLNWAQAALQLNHYPVCPEPDRAMGLAAHTD 234

Query: 204 TSALTILYQ-TTKGLQVSIEGKGWVDVEPVNGALVVQVGDMLHILTNGMYSPSVHQAVVN 263
           ++ LTILYQ  T GLQV  +  GWV V P  G+LVV VGD+ HIL+NG++   +H+A VN
Sbjct: 235 STLLTILYQNNTAGLQVFRDDLGWVTVPPFPGSLVVNVGDLFHILSNGLFKSVLHRARVN 294

Query: 264 QTDDRISTAYFFGPPPKGEVSPLKKLVTPTQPLRYSTVTWADYLRKKYLLFEETLPSIR 322
           QT  R+S A+ +GP    ++SP+ KLV+P +   Y +VTW +YLR K   F + L  IR
Sbjct: 295 QTRARLSVAFLWGPQSDIKISPVPKLVSPVESPLYQSVTWKEYLRTKATHFNKALSMIR 353

[Close]

BLAST of Chy1G020340 vs. ExPASy Swiss-Prot
Match: Q9ZT84 (Gibberellin 3-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA3OX2 PE=1 SV=2)

HSP 1 Score: 301.6 bits (771), Expect = 1.1e-80
Identity = 146/343 (42.57%), Postives = 204/343 (59.48%), Query Frame = 0


Query: 1   MSDVFKTHDPLNIPTNKNLD---------------------SDSDSIPVIDLSLPNAPAL 60
           +SDVF++H P++IP +   D                     +  +++P+IDLS  +   L
Sbjct: 5   LSDVFRSH-PIHIPLSNPPDFKSLPDSYTWTPKDDLLFSASASDETLPLIDLSDIHVATL 64

Query: 61  MNNAFQTWGVCHVLNHGVPLSLLKSMESFINDLFDLPTSQKLKVTRTPESIAGFGLVPLS 120
           + +A  TWG   + NHGVP  LL  +E     LF LP  +KLK  R+   ++G+G+  ++
Sbjct: 65  VGHACTTWGAFQITNHGVPSRLLDDIEFLTGSLFRLPVQRKLKAARSENGVSGYGVARIA 124

Query: 121 KIYPKRPWGEGFTIIGNPVDHFQKLWPQDCKKYCDFVEEYNKEMKSLCGKLLWLTLGELG 180
             + K+ W EGFT+IG+P+  F+KLWP    KYC+ +EEY + M+ L  KL+W  LG LG
Sbjct: 125 SFFNKKMWSEGFTVIGSPLHDFRKLWPSHHLKYCEIIEEYEEHMQKLAAKLMWFALGSLG 184

Query: 181 ITPEDIHWAGPDGDFKTDNQAIRLNSYPVCPEPDDLIGLPPHSDTSALTILYQ-TTKGLQ 240
           +  +DI WAGP+ DF+     I+LN YP CPEPD  +GL  H+D++ +TILYQ  T GLQ
Sbjct: 185 VEEKDIQWAGPNSDFQGTQAVIQLNHYPKCPEPDRAMGLAAHTDSTLMTILYQNNTAGLQ 244

Query: 241 VSIEGKGWVDVEPVNGALVVQVGDMLHILTNGMYSPSVHQAVVNQTDDRISTAYFFGPPP 300
           V  +  GWV   PV G+LVV VGD+LHILTNG++   +H+A VN    R S AY +GPP 
Sbjct: 245 VFRDDVGWVTAPPVPGSLVVNVGDLLHILTNGIFPSVLHRARVNHVRSRFSMAYLWGPPS 304

Query: 301 KGEVSPLKKLVTPTQPLRYSTVTWADYLRKKYLLFEETLPSIR 322
              +SPL KLV P Q   Y ++TW  YL  K   F ++L  IR
Sbjct: 305 DIMISPLPKLVDPLQSPLYPSLTWKQYLATKATHFNQSLSIIR 346

[Close]

BLAST of Chy1G020340 vs. ExPASy Swiss-Prot
Match: Q9C971 (Gibberellin 3-beta-dioxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=GA3OX4 PE=1 SV=1)

HSP 1 Score: 283.1 bits (723), Expect = 4.2e-75
Identity = 138/313 (44.09%), Postives = 195/313 (62.30%), Query Frame = 0


Query: 18  NLDSDSDSIPVIDLSLPNAPALMNNAFQTWGVCHVLNHGVPLSLLKSMESFINDLFDLPT 77
           + D+  +SIPVIDLS P+   L+ +A +TWG   + NHG+   LL  +ES    LFD+P+
Sbjct: 41  SFDAVEESIPVIDLSNPDVTTLIGDASKTWGAFQIANHGISQKLLDDIESLSKTLFDMPS 100

Query: 78  SQKLKVTRTPESIAGFGLVPLSKIYPKRPWGEGFTIIGNPV-DHFQKLWPQDCKKYCDFV 137
            +KL+   + + ++G+G   +S  + K+ W EGFTI  +   +HF  LWP D  KYC  +
Sbjct: 101 ERKLEAASSDKGVSGYGEPRISPFFEKKMWSEGFTIADDSYRNHFNTLWPHDHTKYCGII 160

Query: 138 EEYNKEMKSLCGKLLWLTLGELGITPEDIHWAG--PDGDFKTDNQAIRLNSYPVCPEPDD 197
           +EY  EM+ L  +LL+  LG LG+T EDI WA        K    AIRLN YPVCPEP+ 
Sbjct: 161 QEYVDEMEKLASRLLYCILGSLGVTVEDIEWAHKLEKSGSKVGRGAIRLNHYPVCPEPER 220

Query: 198 LIGLPPHSDTSALTILYQT-TKGLQVSIEGKGWVDVEPVNGALVVQVGDMLHILTNGMYS 257
            +GL  H+D++ LTIL+Q+ T GLQV  E  GWV VEP  G LVV +GD+ HIL+NG   
Sbjct: 221 AMGLAAHTDSTILTILHQSNTGGLQVFREESGWVTVEPAPGVLVVNIGDLFHILSNGKIP 280

Query: 258 PSVHQAVVNQTDDRISTAYFFGPPPKG-EVSPLKKLVTPTQPLRYSTVTWADYLRKKYLL 317
             VH+A VN T  RIS AY +G P    +++P+ KL  P +P  Y ++TW +YL+ KY +
Sbjct: 281 SVVHRAKVNHTRSRISIAYLWGGPAGDVQIAPISKLTGPAEPSLYRSITWKEYLQIKYEV 340

Query: 318 FEETLPSIRLSAP 326
           F++ + +IR+  P
Sbjct: 341 FDKAMDAIRVVNP 353

[Close]

BLAST of Chy1G020340 vs. ExPASy Swiss-Prot
Match: Q9SVS8 (Gibberellin 3-beta-dioxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=GA3OX3 PE=2 SV=1)

HSP 1 Score: 249.2 bits (635), Expect = 6.7e-65
Identity = 126/310 (40.65%), Postives = 185/310 (59.68%), Query Frame = 0


Query: 20  DSDSDSIPVIDLSLPNAPALMNNAFQTWGVCHVLNHGVPLSLLKSMESFINDLFDLPTSQ 79
           ++ S  IPVI LS P    L+  A + WGV H+ +HGV  SLL +++  +  LF LP  +
Sbjct: 41  ETTSGPIPVISLSNPEEHGLLRQACEEWGVFHITDHGVSHSLLHNVDCQMKRLFSLPMHR 100

Query: 80  KLKVTRTPESIAGFGLVPLSKIYPKRPWGEGFTIIGNPV-DHFQKLWPQDCKKYCDFVEE 139
           K+   R+P+   G+G+V +S  Y K  W EGF+++G+ +  H   LWP D  ++C+ +EE
Sbjct: 101 KILAVRSPDESTGYGVVRISMFYDKLMWSEGFSVMGSSLRRHATLLWPDDHAEFCNVMEE 160

Query: 140 YNKEMKSLCGKLLWLTLGELGITPEDIHWAGPDGDFK-TDN--QAIRLNSYPVCPEPDDL 199
           Y K M  L  +L+ + +G LG+T ED+ W  PD     TD+    ++LNSYPVCP+P   
Sbjct: 161 YQKAMDDLSHRLISMLMGSLGLTHEDLGWLVPDKTGSGTDSIQSFLQLNSYPVCPDPHLA 220

Query: 200 IGLPPHSDTSALTILYQ-TTKGLQVSI---EGKGWVDVEPVNGALVVQVGDMLHILTNGM 259
           +GL PH+D+S LTILYQ    GL++     EG  W+ VEP+ G+LVV +GD+ HI++NG 
Sbjct: 221 MGLAPHTDSSLLTILYQGNIPGLEIESPQEEGSRWIGVEPIEGSLVVIMGDLSHIISNGQ 280

Query: 260 YSPSVHQAVVNQTDDRISTAYFFGPPPKGEVSPLKKLVTPTQPLRYSTVTWADYLRKKYL 319
           +  ++H+AVVN+T  R+S AYF GPP   ++ PL        P  Y  + W +YL  K  
Sbjct: 281 FRSTMHRAVVNKTHHRVSAAYFAGPPKNLQIGPLTS--DKNHPPIYRRLIWEEYLAAKAT 340

Query: 320 LFEETLPSIR 322
            F + L   R
Sbjct: 341 HFNKALTLFR 348

[Close]

BLAST of Chy1G020340 vs. ExPASy TrEMBL
Match: A0A6H2MW86 (Gibberellin 9,11-dehydrogenase OS=Cucumis sativus OX=3659 GN=GA9,11dh PE=2 SV=1)

HSP 1 Score: 674.1 bits (1738), Expect = 3.1e-190
Identity = 318/342 (92.98%), Postives = 330/342 (96.49%), Query Frame = 0


Query: 1   MSDVFKTHDPLNIPTNKNLDSDSDSIPVIDLSLPNAPALMNNAFQTWGVCHVLNHGVPLS 60
           MSDVFKTHDPLNIPTNK+LDSDSDSIPVIDLSLPNAPALMNNAF+T G   VLNHGVPLS
Sbjct: 1   MSDVFKTHDPLNIPTNKSLDSDSDSIPVIDLSLPNAPALMNNAFKTCGAFQVLNHGVPLS 60

Query: 61  LLKSMESFINDLFDLPTSQKLKVTRTPESIAGFGLVPLSKIYPKRPWGEGFTIIGNPVDH 120
           LLKSMESFINDLFDLPTSQKLKV R+PESI+GFGLVPLSKIYPKRPWGEGFTIIGNPVDH
Sbjct: 61  LLKSMESFINDLFDLPTSQKLKVVRSPESISGFGLVPLSKIYPKRPWGEGFTIIGNPVDH 120

Query: 121 FQKLWPQDCKKYCDFVEEYNKEMKSLCGKLLWLTLGELGITPEDIHWAGPDGDFKTDNQA 180
            QKLWPQDCKKYCD VEEYNKEMKSLCGKLLWLTLGELGITPEDI+WAGPDGDFKT+NQA
Sbjct: 121 LQKLWPQDCKKYCDLVEEYNKEMKSLCGKLLWLTLGELGITPEDIYWAGPDGDFKTNNQA 180

Query: 181 IRLNSYPVCPEPDDLIGLPPHSDTSALTILYQTTKGLQVSIEGKGWVDVEPVNGALVVQV 240
           IR+NSYPVCPEPDDLIGLPPHSDTSALTILYQTTKGLQVS+EGKGWVDVEP+NGALVVQV
Sbjct: 181 IRMNSYPVCPEPDDLIGLPPHSDTSALTILYQTTKGLQVSMEGKGWVDVEPINGALVVQV 240

Query: 241 GDMLHILTNGMYSPSVHQAVVNQTDDRISTAYFFGPPPKGEVSPLKKLVTPTQPLRYSTV 300
           GDMLHILTNGMY PSVHQAVVNQT DRISTAYFFGPPPKGEVSPLKKLVTPTQPLRY TV
Sbjct: 241 GDMLHILTNGMYPPSVHQAVVNQTSDRISTAYFFGPPPKGEVSPLKKLVTPTQPLRYPTV 300

Query: 301 TWADYLRKKYLLFEETLPSIRLSAPAPTGLSIGNDQNLVKVG 343
           TWADYLRKKY+L+E+ LPSIRLSAPAPTGLS GNDQNLVKVG
Sbjct: 301 TWADYLRKKYVLYEKALPSIRLSAPAPTGLSNGNDQNLVKVG 342

[Close]

BLAST of Chy1G020340 vs. ExPASy TrEMBL
Match: A0A5A7UYZ1 (Gibberellin 3-beta-dioxygenase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G002430 PE=3 SV=1)

HSP 1 Score: 572.8 bits (1475), Expect = 9.7e-160
Identity = 274/349 (78.51%), Postives = 303/349 (86.82%), Query Frame = 0


Query: 1   MSDVFKTHDPLNIPTNKNLDS-------DSDSIPVIDLSLPNAPALMNNAFQTWGVCHVL 60
           MSDV K H  LNIPTNKNLDS        SD++PVIDLS P+AP LMNNAFQTWGV  ++
Sbjct: 1   MSDVSKIHS-LNIPTNKNLDSFQQQLPDASDTVPVIDLSHPDAPKLMNNAFQTWGVFQLV 60

Query: 61  NHGVPLSLLKSMESFINDLFDLPTSQKLKVTRTPESIAGFGLVPLSKIYPKRPWGEGFTI 120
           NHG+P+SLL S++SFI DLFDLPTSQKLK+ R+ ESIAGFGLVPLS++YPKRPWGEGFT+
Sbjct: 61  NHGIPVSLLNSIQSFIQDLFDLPTSQKLKIVRSRESIAGFGLVPLSQVYPKRPWGEGFTL 120

Query: 121 IGNPVDHFQKLWPQDCKKYCDFVEEYNKEMKSLCGKLLWLTLGELGITPEDIHWAGPDGD 180
           IGNPVDH QKLWP+DCKKYCD  EEY KEMK+LCGK++WLTLGELGITPEDI+WAG DG+
Sbjct: 121 IGNPVDHLQKLWPEDCKKYCDLAEEYLKEMKNLCGKVMWLTLGELGITPEDINWAGSDGE 180

Query: 181 FKTDNQAIRLNSYPVCPEPDDLIGLPPHSDTSALTILYQTTKGLQVSIEGKGWVDVEPVN 240
           FKT NQA+R+NSYPVCPEPD+ IGLPPHSD+S LT+LYQTTKGLQV IEGKGWV VEPV 
Sbjct: 181 FKTSNQALRMNSYPVCPEPDESIGLPPHSDSSVLTVLYQTTKGLQVLIEGKGWVTVEPVK 240

Query: 241 GALVVQVGDMLHILTNGMYSPSVHQAVVNQTDDRISTAYFFGPPPKGEVSPLKKLVTPTQ 300
            ALVVQVGDMLHILTNG+YSPSVHQAVVNQT DR+STA+FFG P   EVSPLKKLVTPT+
Sbjct: 241 DALVVQVGDMLHILTNGLYSPSVHQAVVNQTHDRMSTAFFFGTPKNTEVSPLKKLVTPTR 300

Query: 301 PLRYSTVTWADYLRKKYLLFEETLPSIRLSAPAPTGLSIGNDQNLVKVG 343
           PL Y TVTWADYL KKY LFEETLPSIRLSAP P GLS  NDQN VKVG
Sbjct: 301 PLMYPTVTWADYLSKKYELFEETLPSIRLSAP-PPGLSNVNDQNQVKVG 347

[Close]

BLAST of Chy1G020340 vs. ExPASy TrEMBL
Match: A0A1S3CEN9 (gibberellin 3-beta-dioxygenase 1-like OS=Cucumis melo OX=3656 GN=LOC103500014 PE=3 SV=1)

HSP 1 Score: 572.8 bits (1475), Expect = 9.7e-160
Identity = 274/349 (78.51%), Postives = 303/349 (86.82%), Query Frame = 0


Query: 1   MSDVFKTHDPLNIPTNKNLDS-------DSDSIPVIDLSLPNAPALMNNAFQTWGVCHVL 60
           MSDV K H  LNIPTNKNLDS        SD++PVIDLS P+AP LMNNAFQTWGV  ++
Sbjct: 7   MSDVSKIHS-LNIPTNKNLDSFQQQLPDASDTVPVIDLSHPDAPKLMNNAFQTWGVFQLV 66

Query: 61  NHGVPLSLLKSMESFINDLFDLPTSQKLKVTRTPESIAGFGLVPLSKIYPKRPWGEGFTI 120
           NHG+P+SLL S++SFI DLFDLPTSQKLK+ R+ ESIAGFGLVPLS++YPKRPWGEGFT+
Sbjct: 67  NHGIPVSLLNSIQSFIQDLFDLPTSQKLKIVRSRESIAGFGLVPLSQVYPKRPWGEGFTL 126

Query: 121 IGNPVDHFQKLWPQDCKKYCDFVEEYNKEMKSLCGKLLWLTLGELGITPEDIHWAGPDGD 180
           IGNPVDH QKLWP+DCKKYCD  EEY KEMK+LCGK++WLTLGELGITPEDI+WAG DG+
Sbjct: 127 IGNPVDHLQKLWPEDCKKYCDLAEEYLKEMKNLCGKVMWLTLGELGITPEDINWAGSDGE 186

Query: 181 FKTDNQAIRLNSYPVCPEPDDLIGLPPHSDTSALTILYQTTKGLQVSIEGKGWVDVEPVN 240
           FKT NQA+R+NSYPVCPEPD+ IGLPPHSD+S LT+LYQTTKGLQV IEGKGWV VEPV 
Sbjct: 187 FKTSNQALRMNSYPVCPEPDESIGLPPHSDSSVLTVLYQTTKGLQVLIEGKGWVTVEPVK 246

Query: 241 GALVVQVGDMLHILTNGMYSPSVHQAVVNQTDDRISTAYFFGPPPKGEVSPLKKLVTPTQ 300
            ALVVQVGDMLHILTNG+YSPSVHQAVVNQT DR+STA+FFG P   EVSPLKKLVTPT+
Sbjct: 247 DALVVQVGDMLHILTNGLYSPSVHQAVVNQTHDRMSTAFFFGTPKNTEVSPLKKLVTPTR 306

Query: 301 PLRYSTVTWADYLRKKYLLFEETLPSIRLSAPAPTGLSIGNDQNLVKVG 343
           PL Y TVTWADYL KKY LFEETLPSIRLSAP P GLS  NDQN VKVG
Sbjct: 307 PLMYPTVTWADYLSKKYELFEETLPSIRLSAP-PPGLSNVNDQNQVKVG 353

[Close]

BLAST of Chy1G020340 vs. ExPASy TrEMBL
Match: S0BE27 (GA 3-oxidase OS=Cucumis sativus OX=3659 GN=GA3ox3 PE=2 SV=1)

HSP 1 Score: 461.5 bits (1186), Expect = 3.2e-126
Identity = 228/368 (61.96%), Postives = 267/368 (72.55%), Query Frame = 0


Query: 1   MSDVFKTHDPLNIPTNK-NLD-------------------------SDSDSIPVIDLSLP 60
           ++D++KTH PL+IPTNK NLD                         S SDS+P+IDLSLP
Sbjct: 9   LTDIYKTH-PLDIPTNKLNLDSLQQLPDTYDWIQPDAFPSFRDPNISLSDSVPLIDLSLP 68

Query: 61  NAPALMNNAFQTWGVCHVLNHGVPLSLLKSMESFINDLFDLPTSQKLKVTRTPESIAGFG 120
           NAP L+ NA +TWGV  V+NHGVP+SLL SMESF N LFDLP+  KLK  RTP+ + G+G
Sbjct: 69  NAPHLIGNALKTWGVFQVINHGVPISLLNSMESFTNTLFDLPSPHKLKAARTPDGVTGYG 128

Query: 121 LVPLSKIYPKRPWGEGFTIIGNPVDHFQKLWPQDCKKYCDFVEEYNKEMKSLCGKLLWLT 180
           L  +S  +PKR W EGFTI+G+P++HFQKLWP +  KYCD  EEY++EMKSLCG+++WL 
Sbjct: 129 LPRISTFFPKRMWSEGFTIVGSPLEHFQKLWPNEYSKYCDITEEYSREMKSLCGRVIWLA 188

Query: 181 LGELGITPEDIHWAGPDGDFKTDNQAIRLNSYPVCPEPDDLIGLPPHSDTSALTILYQ-T 240
           LGELGIT ED++WA  +GDFKT N  I+LNSYPVCP+PD  +GL  H+DTS LTILYQ  
Sbjct: 189 LGELGITREDVNWAEANGDFKTSNAVIQLNSYPVCPDPDRAMGLGAHTDTSLLTILYQNN 248

Query: 241 TKGLQVSIEGKGWVDVEPVNGALVVQVGDMLHILTNGMYSPSVHQAVVNQTDDRISTAYF 300
           T+GLQV  EG  WV VEPV GALVVQVGD+LHILTNG+Y  S HQAVVNQ   RIS AYF
Sbjct: 249 TRGLQVLREGNRWVMVEPVTGALVVQVGDLLHILTNGLYPSSAHQAVVNQNRKRISIAYF 308

Query: 301 FGPPPKGEVSPLKKLVTPTQPLRYSTVTWADYLRKKYLLFEETLPSIRLSAPAPTGLSIG 342
           FGP    E+SPL KLVTPTQPL Y TVTW +YLRKK  LF  TL SIRLSAP    L I 
Sbjct: 309 FGPSESAEISPLNKLVTPTQPLLYPTVTWTEYLRKKAQLFNNTLSSIRLSAPLTGALDI- 368

[Close]

BLAST of Chy1G020340 vs. ExPASy TrEMBL
Match: A0A5A7UX94 (Gibberellin 3-beta-dioxygenase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G002440 PE=3 SV=1)

HSP 1 Score: 457.2 bits (1175), Expect = 6.0e-125
Identity = 227/371 (61.19%), Postives = 273/371 (73.58%), Query Frame = 0


Query: 1   MSDVFKTHDPLNIPTNKNLDSD--------------------------SDSIPVIDLS-L 60
           ++DV+KTH PL+IPTNKNLDS                           SDS+P+IDLS L
Sbjct: 7   LTDVYKTH-PLDIPTNKNLDSFEQLPDTYDWIQPDSFPSSLTDPNVSLSDSVPLIDLSDL 66

Query: 61  PNAPALMNNAFQTWGVCHVLNHGVPLSLLKSMESFINDLFDLPTSQKLKVTRTPESIAGF 120
           PNAP L+ NA +TWGV  V+NHGVP+SLL SMESF N LFDLP+  KLK  RTP+ +AG+
Sbjct: 67  PNAPHLIGNALKTWGVFQVINHGVPISLLNSMESFTNALFDLPSPHKLKAARTPDGVAGY 126

Query: 121 GLVPLSKIYPKRPWGEGFTIIGNPVDHFQKLWPQDCKKYCDFVEEYNKEMKSLCGKLLWL 180
           GL  +S  +PKR W EGFTI+G+P++HFQKLWP +  KYCD  EEY++EM SLCG+L+WL
Sbjct: 127 GLPRISTFFPKRMWSEGFTIVGSPLEHFQKLWPDEYSKYCDLTEEYSREMGSLCGRLIWL 186

Query: 181 TLGELGITPEDIHWAGPDGDFKTD-NQAIRLNSYPVCPEPDDLIGLPPHSDTSALTILYQ 240
            LGELGIT ED++WAG +GDFKT  N  I+LNSYP+CP+PD  +GL  H+D+S LTILYQ
Sbjct: 187 ALGELGITQEDVNWAGSNGDFKTSTNAVIQLNSYPICPDPDRAMGLGAHTDSSLLTILYQ 246

Query: 241 -TTKGLQVSIEGKGWVDVEPVNGALVVQVGDMLHILTNGMYSPSVHQAVVNQTDDRISTA 300
             ++GLQV  EG  WV VEPVNGALVVQVGD+LHILTNG+Y  SVHQAVVNQT  RIS A
Sbjct: 247 NNSRGLQVLREGNRWVMVEPVNGALVVQVGDLLHILTNGLYLSSVHQAVVNQTQKRISIA 306

Query: 301 YFFGPPPKGEVSPLKKLVTPTQPLRYSTVTWADYLRKKYLLFEETLPSIRLSAPAPTGLS 343
           YFFGP    E+SP+ KLVTPT+PL Y T+TW +YLRKK  LF  TL SIRL AP P+G+ 
Sbjct: 307 YFFGPSEGVEISPINKLVTPTRPLLYPTITWTEYLRKKAKLFNNTLSSIRLCAP-PSGVL 366

[Close]

BLAST of Chy1G020340 vs. NCBI nr
Match: KAE8646457.1 (hypothetical protein Csa_015524 [Cucumis sativus] >SLM22339.1 Gibberellin 9,11-dehydrogenase [Cucumis sativus])

HSP 1 Score: 667 bits (1722), Expect = 1.25e-241
Identity = 318/342 (92.98%), Postives = 330/342 (96.49%), Query Frame = 0


Query: 1   MSDVFKTHDPLNIPTNKNLDSDSDSIPVIDLSLPNAPALMNNAFQTWGVCHVLNHGVPLS 60
           MSDVFKTHDPLNIPTNK+LDSDSDSIPVIDLSLPNAPALMNNAF+T G   VLNHGVPLS
Sbjct: 1   MSDVFKTHDPLNIPTNKSLDSDSDSIPVIDLSLPNAPALMNNAFKTCGAFQVLNHGVPLS 60

Query: 61  LLKSMESFINDLFDLPTSQKLKVTRTPESIAGFGLVPLSKIYPKRPWGEGFTIIGNPVDH 120
           LLKSMESFINDLFDLPTSQKLKV R+PESI+GFGLVPLSKIYPKRPWGEGFTIIGNPVDH
Sbjct: 61  LLKSMESFINDLFDLPTSQKLKVVRSPESISGFGLVPLSKIYPKRPWGEGFTIIGNPVDH 120

Query: 121 FQKLWPQDCKKYCDFVEEYNKEMKSLCGKLLWLTLGELGITPEDIHWAGPDGDFKTDNQA 180
            QKLWPQDCKKYCD VEEYNKEMKSLCGKLLWLTLGELGITPEDI+WAGPDGDFKT+NQA
Sbjct: 121 LQKLWPQDCKKYCDLVEEYNKEMKSLCGKLLWLTLGELGITPEDIYWAGPDGDFKTNNQA 180

Query: 181 IRLNSYPVCPEPDDLIGLPPHSDTSALTILYQTTKGLQVSIEGKGWVDVEPVNGALVVQV 240
           IR+NSYPVCPEPDDLIGLPPHSDTSALTILYQTTKGLQVS+EGKGWVDVEP+NGALVVQV
Sbjct: 181 IRMNSYPVCPEPDDLIGLPPHSDTSALTILYQTTKGLQVSMEGKGWVDVEPINGALVVQV 240

Query: 241 GDMLHILTNGMYSPSVHQAVVNQTDDRISTAYFFGPPPKGEVSPLKKLVTPTQPLRYSTV 300
           GDMLHILTNGMY PSVHQAVVNQT DRISTAYFFGPPPKGEVSPLKKLVTPTQPLRY TV
Sbjct: 241 GDMLHILTNGMYPPSVHQAVVNQTSDRISTAYFFGPPPKGEVSPLKKLVTPTQPLRYPTV 300

Query: 301 TWADYLRKKYLLFEETLPSIRLSAPAPTGLSIGNDQNLVKVG 342
           TWADYLRKKY+L+E+ LPSIRLSAPAPTGLS GNDQNLVKVG
Sbjct: 301 TWADYLRKKYVLYEKALPSIRLSAPAPTGLSNGNDQNLVKVG 342

[Close]

BLAST of Chy1G020340 vs. NCBI nr
Match: XP_004136071.2 (gibberellin 3-beta-dioxygenase 1 [Cucumis sativus])

HSP 1 Score: 667 bits (1722), Expect = 1.56e-241
Identity = 318/342 (92.98%), Postives = 330/342 (96.49%), Query Frame = 0


Query: 1   MSDVFKTHDPLNIPTNKNLDSDSDSIPVIDLSLPNAPALMNNAFQTWGVCHVLNHGVPLS 60
           MSDVFKTHDPLNIPTNK+LDSDSDSIPVIDLSLPNAPALMNNAF+T G   VLNHGVPLS
Sbjct: 7   MSDVFKTHDPLNIPTNKSLDSDSDSIPVIDLSLPNAPALMNNAFKTCGAFQVLNHGVPLS 66

Query: 61  LLKSMESFINDLFDLPTSQKLKVTRTPESIAGFGLVPLSKIYPKRPWGEGFTIIGNPVDH 120
           LLKSMESFINDLFDLPTSQKLKV R+PESI+GFGLVPLSKIYPKRPWGEGFTIIGNPVDH
Sbjct: 67  LLKSMESFINDLFDLPTSQKLKVVRSPESISGFGLVPLSKIYPKRPWGEGFTIIGNPVDH 126

Query: 121 FQKLWPQDCKKYCDFVEEYNKEMKSLCGKLLWLTLGELGITPEDIHWAGPDGDFKTDNQA 180
            QKLWPQDCKKYCD VEEYNKEMKSLCGKLLWLTLGELGITPEDI+WAGPDGDFKT+NQA
Sbjct: 127 LQKLWPQDCKKYCDLVEEYNKEMKSLCGKLLWLTLGELGITPEDIYWAGPDGDFKTNNQA 186

Query: 181 IRLNSYPVCPEPDDLIGLPPHSDTSALTILYQTTKGLQVSIEGKGWVDVEPVNGALVVQV 240
           IR+NSYPVCPEPDDLIGLPPHSDTSALTILYQTTKGLQVS+EGKGWVDVEP+NGALVVQV
Sbjct: 187 IRMNSYPVCPEPDDLIGLPPHSDTSALTILYQTTKGLQVSMEGKGWVDVEPINGALVVQV 246

Query: 241 GDMLHILTNGMYSPSVHQAVVNQTDDRISTAYFFGPPPKGEVSPLKKLVTPTQPLRYSTV 300
           GDMLHILTNGMY PSVHQAVVNQT DRISTAYFFGPPPKGEVSPLKKLVTPTQPLRY TV
Sbjct: 247 GDMLHILTNGMYPPSVHQAVVNQTSDRISTAYFFGPPPKGEVSPLKKLVTPTQPLRYPTV 306

Query: 301 TWADYLRKKYLLFEETLPSIRLSAPAPTGLSIGNDQNLVKVG 342
           TWADYLRKKY+L+E+ LPSIRLSAPAPTGLS GNDQNLVKVG
Sbjct: 307 TWADYLRKKYVLYEKALPSIRLSAPAPTGLSNGNDQNLVKVG 348

[Close]

BLAST of Chy1G020340 vs. NCBI nr
Match: KAA0058795.1 (gibberellin 3-beta-dioxygenase 1-like [Cucumis melo var. makuwa] >TYK10589.1 gibberellin 3-beta-dioxygenase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 566 bits (1459), Expect = 1.77e-201
Identity = 274/349 (78.51%), Postives = 303/349 (86.82%), Query Frame = 0


Query: 1   MSDVFKTHDPLNIPTNKNLDS-------DSDSIPVIDLSLPNAPALMNNAFQTWGVCHVL 60
           MSDV K H  LNIPTNKNLDS        SD++PVIDLS P+AP LMNNAFQTWGV  ++
Sbjct: 1   MSDVSKIHS-LNIPTNKNLDSFQQQLPDASDTVPVIDLSHPDAPKLMNNAFQTWGVFQLV 60

Query: 61  NHGVPLSLLKSMESFINDLFDLPTSQKLKVTRTPESIAGFGLVPLSKIYPKRPWGEGFTI 120
           NHG+P+SLL S++SFI DLFDLPTSQKLK+ R+ ESIAGFGLVPLS++YPKRPWGEGFT+
Sbjct: 61  NHGIPVSLLNSIQSFIQDLFDLPTSQKLKIVRSRESIAGFGLVPLSQVYPKRPWGEGFTL 120

Query: 121 IGNPVDHFQKLWPQDCKKYCDFVEEYNKEMKSLCGKLLWLTLGELGITPEDIHWAGPDGD 180
           IGNPVDH QKLWP+DCKKYCD  EEY KEMK+LCGK++WLTLGELGITPEDI+WAG DG+
Sbjct: 121 IGNPVDHLQKLWPEDCKKYCDLAEEYLKEMKNLCGKVMWLTLGELGITPEDINWAGSDGE 180

Query: 181 FKTDNQAIRLNSYPVCPEPDDLIGLPPHSDTSALTILYQTTKGLQVSIEGKGWVDVEPVN 240
           FKT NQA+R+NSYPVCPEPD+ IGLPPHSD+S LT+LYQTTKGLQV IEGKGWV VEPV 
Sbjct: 181 FKTSNQALRMNSYPVCPEPDESIGLPPHSDSSVLTVLYQTTKGLQVLIEGKGWVTVEPVK 240

Query: 241 GALVVQVGDMLHILTNGMYSPSVHQAVVNQTDDRISTAYFFGPPPKGEVSPLKKLVTPTQ 300
            ALVVQVGDMLHILTNG+YSPSVHQAVVNQT DR+STA+FFG P   EVSPLKKLVTPT+
Sbjct: 241 DALVVQVGDMLHILTNGLYSPSVHQAVVNQTHDRMSTAFFFGTPKNTEVSPLKKLVTPTR 300

Query: 301 PLRYSTVTWADYLRKKYLLFEETLPSIRLSAPAPTGLSIGNDQNLVKVG 342
           PL Y TVTWADYL KKY LFEETLPSIRLSAP P GLS  NDQN VKVG
Sbjct: 301 PLMYPTVTWADYLSKKYELFEETLPSIRLSAPPP-GLSNVNDQNQVKVG 347

[Close]

BLAST of Chy1G020340 vs. NCBI nr
Match: XP_008461418.1 (PREDICTED: gibberellin 3-beta-dioxygenase 1-like [Cucumis melo])

HSP 1 Score: 566 bits (1459), Expect = 2.21e-201
Identity = 274/349 (78.51%), Postives = 303/349 (86.82%), Query Frame = 0


Query: 1   MSDVFKTHDPLNIPTNKNLDS-------DSDSIPVIDLSLPNAPALMNNAFQTWGVCHVL 60
           MSDV K H  LNIPTNKNLDS        SD++PVIDLS P+AP LMNNAFQTWGV  ++
Sbjct: 7   MSDVSKIHS-LNIPTNKNLDSFQQQLPDASDTVPVIDLSHPDAPKLMNNAFQTWGVFQLV 66

Query: 61  NHGVPLSLLKSMESFINDLFDLPTSQKLKVTRTPESIAGFGLVPLSKIYPKRPWGEGFTI 120
           NHG+P+SLL S++SFI DLFDLPTSQKLK+ R+ ESIAGFGLVPLS++YPKRPWGEGFT+
Sbjct: 67  NHGIPVSLLNSIQSFIQDLFDLPTSQKLKIVRSRESIAGFGLVPLSQVYPKRPWGEGFTL 126

Query: 121 IGNPVDHFQKLWPQDCKKYCDFVEEYNKEMKSLCGKLLWLTLGELGITPEDIHWAGPDGD 180
           IGNPVDH QKLWP+DCKKYCD  EEY KEMK+LCGK++WLTLGELGITPEDI+WAG DG+
Sbjct: 127 IGNPVDHLQKLWPEDCKKYCDLAEEYLKEMKNLCGKVMWLTLGELGITPEDINWAGSDGE 186

Query: 181 FKTDNQAIRLNSYPVCPEPDDLIGLPPHSDTSALTILYQTTKGLQVSIEGKGWVDVEPVN 240
           FKT NQA+R+NSYPVCPEPD+ IGLPPHSD+S LT+LYQTTKGLQV IEGKGWV VEPV 
Sbjct: 187 FKTSNQALRMNSYPVCPEPDESIGLPPHSDSSVLTVLYQTTKGLQVLIEGKGWVTVEPVK 246

Query: 241 GALVVQVGDMLHILTNGMYSPSVHQAVVNQTDDRISTAYFFGPPPKGEVSPLKKLVTPTQ 300
            ALVVQVGDMLHILTNG+YSPSVHQAVVNQT DR+STA+FFG P   EVSPLKKLVTPT+
Sbjct: 247 DALVVQVGDMLHILTNGLYSPSVHQAVVNQTHDRMSTAFFFGTPKNTEVSPLKKLVTPTR 306

Query: 301 PLRYSTVTWADYLRKKYLLFEETLPSIRLSAPAPTGLSIGNDQNLVKVG 342
           PL Y TVTWADYL KKY LFEETLPSIRLSAP P GLS  NDQN VKVG
Sbjct: 307 PLMYPTVTWADYLSKKYELFEETLPSIRLSAPPP-GLSNVNDQNQVKVG 353

[Close]

BLAST of Chy1G020340 vs. NCBI nr
Match: NP_001267625.1 (gibberellin 3-beta-dioxygenase 1-like [Cucumis sativus] >KGN45325.1 hypothetical protein Csa_016321 [Cucumis sativus] >CCD28484.1 GA 3-oxidase [Cucumis sativus])

HSP 1 Score: 455 bits (1170), Expect = 4.39e-157
Identity = 228/368 (61.96%), Postives = 267/368 (72.55%), Query Frame = 0


Query: 1   MSDVFKTHDPLNIPTNK-NLDSD-------------------------SDSIPVIDLSLP 60
           ++D++KTH PL+IPTNK NLDS                          SDS+P+IDLSLP
Sbjct: 9   LTDIYKTH-PLDIPTNKLNLDSLQQLPDTYDWIQPDAFPSFRDPNISLSDSVPLIDLSLP 68

Query: 61  NAPALMNNAFQTWGVCHVLNHGVPLSLLKSMESFINDLFDLPTSQKLKVTRTPESIAGFG 120
           NAP L+ NA +TWGV  V+NHGVP+SLL SMESF N LFDLP+  KLK  RTP+ + G+G
Sbjct: 69  NAPHLIGNALKTWGVFQVINHGVPISLLNSMESFTNTLFDLPSPHKLKAARTPDGVTGYG 128

Query: 121 LVPLSKIYPKRPWGEGFTIIGNPVDHFQKLWPQDCKKYCDFVEEYNKEMKSLCGKLLWLT 180
           L  +S  +PKR W EGFTI+G+P++HFQKLWP +  KYCD  EEY++EMKSLCG+++WL 
Sbjct: 129 LPRISTFFPKRMWSEGFTIVGSPLEHFQKLWPNEYSKYCDITEEYSREMKSLCGRVIWLA 188

Query: 181 LGELGITPEDIHWAGPDGDFKTDNQAIRLNSYPVCPEPDDLIGLPPHSDTSALTILYQT- 240
           LGELGIT ED++WA  +GDFKT N  I+LNSYPVCP+PD  +GL  H+DTS LTILYQ  
Sbjct: 189 LGELGITREDVNWAEANGDFKTSNAVIQLNSYPVCPDPDRAMGLGAHTDTSLLTILYQNN 248

Query: 241 TKGLQVSIEGKGWVDVEPVNGALVVQVGDMLHILTNGMYSPSVHQAVVNQTDDRISTAYF 300
           T+GLQV  EG  WV VEPV GALVVQVGD+LHILTNG+Y  S HQAVVNQ   RIS AYF
Sbjct: 249 TRGLQVLREGNRWVMVEPVTGALVVQVGDLLHILTNGLYPSSAHQAVVNQNRKRISIAYF 308

Query: 301 FGPPPKGEVSPLKKLVTPTQPLRYSTVTWADYLRKKYLLFEETLPSIRLSAPAPTGLSIG 341
           FGP    E+SPL KLVTPTQPL Y TVTW +YLRKK  LF  TL SIRLSAP    L I 
Sbjct: 309 FGPSESAEISPLNKLVTPTQPLLYPTVTWTEYLRKKAQLFNNTLSSIRLSAPLTGALDI- 368

[Close]

BLAST of Chy1G020340 vs. TAIR 10
Match: AT1G15550.1 (gibberellin 3-oxidase 1 )

HSP 1 Score: 302.0 bits (772), Expect = 6.2e-82
Identity = 140/299 (46.82%), Postives = 193/299 (64.55%), Query Frame = 0


Query: 24  DSIPVIDLSLPNAPALMNNAFQTWGVCHVLNHGVPLSLLKSMESFINDLFDLPTSQKLKV 83
           ++IP+IDL  P+A   + +A +TWG   + NHGVPL LL+ +E     LF LP  +KLK 
Sbjct: 55  ENIPLIDLDHPDATNQIGHACRTWGAFQISNHGVPLGLLQDIEFLTGSLFGLPVQRKLKS 114

Query: 84  TRTPESIAGFGLVPLSKIYPKRPWGEGFTIIGNPVDHFQKLWPQDCKKYCDFVEEYNKEM 143
            R+   ++G+G+  ++  + K+ W EGFTI G+P++ F+KLWPQ    YCD VEEY + M
Sbjct: 115 ARSETGVSGYGVARIASFFNKQMWSEGFTITGSPLNDFRKLWPQHHLNYCDIVEEYEEHM 174

Query: 144 KSLCGKLLWLTLGELGITPEDIHWAGPDGDFKTDNQAIRLNSYPVCPEPDDLIGLPPHSD 203
           K L  KL+WL L  LG++ EDI WA    D      A++LN YPVCPEPD  +GL  H+D
Sbjct: 175 KKLASKLMWLALNSLGVSEEDIEWASLSSDLNWAQAALQLNHYPVCPEPDRAMGLAAHTD 234

Query: 204 TSALTILYQ-TTKGLQVSIEGKGWVDVEPVNGALVVQVGDMLHILTNGMYSPSVHQAVVN 263
           ++ LTILYQ  T GLQV  +  GWV V P  G+LVV VGD+ HIL+NG++   +H+A VN
Sbjct: 235 STLLTILYQNNTAGLQVFRDDLGWVTVPPFPGSLVVNVGDLFHILSNGLFKSVLHRARVN 294

Query: 264 QTDDRISTAYFFGPPPKGEVSPLKKLVTPTQPLRYSTVTWADYLRKKYLLFEETLPSIR 322
           QT  R+S A+ +GP    ++SP+ KLV+P +   Y +VTW +YLR K   F + L  IR
Sbjct: 295 QTRARLSVAFLWGPQSDIKISPVPKLVSPVESPLYQSVTWKEYLRTKATHFNKALSMIR 353

[Close]

BLAST of Chy1G020340 vs. TAIR 10
Match: AT1G80340.1 (gibberellin 3-oxidase 2 )

HSP 1 Score: 301.6 bits (771), Expect = 8.1e-82
Identity = 146/343 (42.57%), Postives = 204/343 (59.48%), Query Frame = 0


Query: 1   MSDVFKTHDPLNIPTNKNLD---------------------SDSDSIPVIDLSLPNAPAL 60
           +SDVF++H P++IP +   D                     +  +++P+IDLS  +   L
Sbjct: 5   LSDVFRSH-PIHIPLSNPPDFKSLPDSYTWTPKDDLLFSASASDETLPLIDLSDIHVATL 64

Query: 61  MNNAFQTWGVCHVLNHGVPLSLLKSMESFINDLFDLPTSQKLKVTRTPESIAGFGLVPLS 120
           + +A  TWG   + NHGVP  LL  +E     LF LP  +KLK  R+   ++G+G+  ++
Sbjct: 65  VGHACTTWGAFQITNHGVPSRLLDDIEFLTGSLFRLPVQRKLKAARSENGVSGYGVARIA 124

Query: 121 KIYPKRPWGEGFTIIGNPVDHFQKLWPQDCKKYCDFVEEYNKEMKSLCGKLLWLTLGELG 180
             + K+ W EGFT+IG+P+  F+KLWP    KYC+ +EEY + M+ L  KL+W  LG LG
Sbjct: 125 SFFNKKMWSEGFTVIGSPLHDFRKLWPSHHLKYCEIIEEYEEHMQKLAAKLMWFALGSLG 184

Query: 181 ITPEDIHWAGPDGDFKTDNQAIRLNSYPVCPEPDDLIGLPPHSDTSALTILYQ-TTKGLQ 240
           +  +DI WAGP+ DF+     I+LN YP CPEPD  +GL  H+D++ +TILYQ  T GLQ
Sbjct: 185 VEEKDIQWAGPNSDFQGTQAVIQLNHYPKCPEPDRAMGLAAHTDSTLMTILYQNNTAGLQ 244

Query: 241 VSIEGKGWVDVEPVNGALVVQVGDMLHILTNGMYSPSVHQAVVNQTDDRISTAYFFGPPP 300
           V  +  GWV   PV G+LVV VGD+LHILTNG++   +H+A VN    R S AY +GPP 
Sbjct: 245 VFRDDVGWVTAPPVPGSLVVNVGDLLHILTNGIFPSVLHRARVNHVRSRFSMAYLWGPPS 304

Query: 301 KGEVSPLKKLVTPTQPLRYSTVTWADYLRKKYLLFEETLPSIR 322
              +SPL KLV P Q   Y ++TW  YL  K   F ++L  IR
Sbjct: 305 DIMISPLPKLVDPLQSPLYPSLTWKQYLATKATHFNQSLSIIR 346

[Close]

BLAST of Chy1G020340 vs. TAIR 10
Match: AT1G80330.1 (gibberellin 3-oxidase 4 )

HSP 1 Score: 283.1 bits (723), Expect = 3.0e-76
Identity = 138/313 (44.09%), Postives = 195/313 (62.30%), Query Frame = 0


Query: 18  NLDSDSDSIPVIDLSLPNAPALMNNAFQTWGVCHVLNHGVPLSLLKSMESFINDLFDLPT 77
           + D+  +SIPVIDLS P+   L+ +A +TWG   + NHG+   LL  +ES    LFD+P+
Sbjct: 41  SFDAVEESIPVIDLSNPDVTTLIGDASKTWGAFQIANHGISQKLLDDIESLSKTLFDMPS 100

Query: 78  SQKLKVTRTPESIAGFGLVPLSKIYPKRPWGEGFTIIGNPV-DHFQKLWPQDCKKYCDFV 137
            +KL+   + + ++G+G   +S  + K+ W EGFTI  +   +HF  LWP D  KYC  +
Sbjct: 101 ERKLEAASSDKGVSGYGEPRISPFFEKKMWSEGFTIADDSYRNHFNTLWPHDHTKYCGII 160

Query: 138 EEYNKEMKSLCGKLLWLTLGELGITPEDIHWAG--PDGDFKTDNQAIRLNSYPVCPEPDD 197
           +EY  EM+ L  +LL+  LG LG+T EDI WA        K    AIRLN YPVCPEP+ 
Sbjct: 161 QEYVDEMEKLASRLLYCILGSLGVTVEDIEWAHKLEKSGSKVGRGAIRLNHYPVCPEPER 220

Query: 198 LIGLPPHSDTSALTILYQT-TKGLQVSIEGKGWVDVEPVNGALVVQVGDMLHILTNGMYS 257
            +GL  H+D++ LTIL+Q+ T GLQV  E  GWV VEP  G LVV +GD+ HIL+NG   
Sbjct: 221 AMGLAAHTDSTILTILHQSNTGGLQVFREESGWVTVEPAPGVLVVNIGDLFHILSNGKIP 280

Query: 258 PSVHQAVVNQTDDRISTAYFFGPPPKG-EVSPLKKLVTPTQPLRYSTVTWADYLRKKYLL 317
             VH+A VN T  RIS AY +G P    +++P+ KL  P +P  Y ++TW +YL+ KY +
Sbjct: 281 SVVHRAKVNHTRSRISIAYLWGGPAGDVQIAPISKLTGPAEPSLYRSITWKEYLQIKYEV 340

Query: 318 FEETLPSIRLSAP 326
           F++ + +IR+  P
Sbjct: 341 FDKAMDAIRVVNP 353

[Close]

BLAST of Chy1G020340 vs. TAIR 10
Match: AT4G21690.1 (gibberellin 3-oxidase 3 )

HSP 1 Score: 249.2 bits (635), Expect = 4.7e-66
Identity = 126/310 (40.65%), Postives = 185/310 (59.68%), Query Frame = 0


Query: 20  DSDSDSIPVIDLSLPNAPALMNNAFQTWGVCHVLNHGVPLSLLKSMESFINDLFDLPTSQ 79
           ++ S  IPVI LS P    L+  A + WGV H+ +HGV  SLL +++  +  LF LP  +
Sbjct: 41  ETTSGPIPVISLSNPEEHGLLRQACEEWGVFHITDHGVSHSLLHNVDCQMKRLFSLPMHR 100

Query: 80  KLKVTRTPESIAGFGLVPLSKIYPKRPWGEGFTIIGNPV-DHFQKLWPQDCKKYCDFVEE 139
           K+   R+P+   G+G+V +S  Y K  W EGF+++G+ +  H   LWP D  ++C+ +EE
Sbjct: 101 KILAVRSPDESTGYGVVRISMFYDKLMWSEGFSVMGSSLRRHATLLWPDDHAEFCNVMEE 160

Query: 140 YNKEMKSLCGKLLWLTLGELGITPEDIHWAGPDGDFK-TDN--QAIRLNSYPVCPEPDDL 199
           Y K M  L  +L+ + +G LG+T ED+ W  PD     TD+    ++LNSYPVCP+P   
Sbjct: 161 YQKAMDDLSHRLISMLMGSLGLTHEDLGWLVPDKTGSGTDSIQSFLQLNSYPVCPDPHLA 220

Query: 200 IGLPPHSDTSALTILYQ-TTKGLQVSI---EGKGWVDVEPVNGALVVQVGDMLHILTNGM 259
           +GL PH+D+S LTILYQ    GL++     EG  W+ VEP+ G+LVV +GD+ HI++NG 
Sbjct: 221 MGLAPHTDSSLLTILYQGNIPGLEIESPQEEGSRWIGVEPIEGSLVVIMGDLSHIISNGQ 280

Query: 260 YSPSVHQAVVNQTDDRISTAYFFGPPPKGEVSPLKKLVTPTQPLRYSTVTWADYLRKKYL 319
           +  ++H+AVVN+T  R+S AYF GPP   ++ PL        P  Y  + W +YL  K  
Sbjct: 281 FRSTMHRAVVNKTHHRVSAAYFAGPPKNLQIGPLTS--DKNHPPIYRRLIWEEYLAAKAT 340

Query: 320 LFEETLPSIR 322
            F + L   R
Sbjct: 341 HFNKALTLFR 348

[Close]

BLAST of Chy1G020340 vs. TAIR 10
Match: AT3G21420.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 177.6 bits (449), Expect = 1.8e-44
Identity = 97/294 (32.99%), Postives = 156/294 (53.06%), Query Frame = 0


Query: 26  IPVIDLSLPNAP---------ALMNNAFQTWGVCHVLNHGVPLSLLKSMESFINDLFDLP 85
           IPVIDLS  + P           ++ A + WG   V+NHG+ + +++ +E   ++ FD+P
Sbjct: 55  IPVIDLSKLSKPDNDDFFFEILKLSQACEDWGFFQVINHGIEVEVVEDIEEVASEFFDMP 114

Query: 86  TSQKLKVTRTPESIAGFGLVPLSKIYPKRPWGEGFTI-IGNPVDHFQKLWPQDCKKYCDF 145
             +K K    P ++ G+G   +     K  W   F + +  P     KLWP    ++ + 
Sbjct: 115 LEEKKKYPMEPGTVQGYGQAFIFSEDQKLDWCNMFALGVHPPQIRNPKLWPSKPARFSES 174

Query: 146 VEEYNKEMKSLCGKLLWLTLGELGITPEDIHWAGPDGDFKTDNQAIRLNSYPVCPEPDDL 205
           +E Y+KE++ LC +LL      LG+  E       +  F    QA+R+N YP C  PD +
Sbjct: 175 LEGYSKEIRELCKRLLKYIAISLGLKEERF-----EEMFGEAVQAVRMNYYPPCSSPDLV 234

Query: 206 IGLPPHSDTSALTILYQTTK---GLQVSIEGKGWVDVEPVNGALVVQVGDMLHILTNGMY 265
           +GL PHSD SALT+L Q+     GLQ+ ++   WV V+P+  ALV+ +GD + +L+NG Y
Sbjct: 235 LGLSPHSDGSALTVLQQSKNSCVGLQI-LKDNTWVPVKPLPNALVINIGDTIEVLSNGKY 294

Query: 266 SPSVHQAVVNQTDDRISTAYFFGPPPKGEVSPLKKLV-TPTQPLRYSTVTWADY 306
               H+AV N+  +R++   F+ P  + E+ P+ +LV   T P +Y +    DY
Sbjct: 295 KSVEHRAVTNREKERLTIVTFYAPNYEVEIEPMSELVDDETNPCKYRSYNHGDY 342

The following BLAST results are available for this feature:

BLAST of Chy1G020340 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O24648	5.4e-91	48.05	Gibberellin 3-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=LE PE=1 SV=1	[more]
Q39103	8.7e-81	46.82	Gibberellin 3-beta-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=GA3OX1 PE=1 ...	[more]
Q9ZT84	1.1e-80	42.57	Gibberellin 3-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA3OX2 PE=1 ...	[more]
Q9C971	4.2e-75	44.09	Gibberellin 3-beta-dioxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=GA3OX4 PE=1 ...	[more]
Q9SVS8	6.7e-65	40.65	Gibberellin 3-beta-dioxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=GA3OX3 PE=2 ...	[more]

BLAST of Chy1G020340 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6H2MW86	3.1e-190	92.98	Gibberellin 9,11-dehydrogenase OS=Cucumis sativus OX=3659 GN=GA9,11dh PE=2 SV=1	[more]
A0A5A7UYZ1	9.7e-160	78.51	Gibberellin 3-beta-dioxygenase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=...	[more]
A0A1S3CEN9	9.7e-160	78.51	gibberellin 3-beta-dioxygenase 1-like OS=Cucumis melo OX=3656 GN=LOC103500014 PE...	[more]
S0BE27	3.2e-126	61.96	GA 3-oxidase OS=Cucumis sativus OX=3659 GN=GA3ox3 PE=2 SV=1	[more]
A0A5A7UX94	6.0e-125	61.19	Gibberellin 3-beta-dioxygenase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=...	[more]

BLAST of Chy1G020340 vs. NCBI nr


Match Name	E-value	Identity	Description
KAE8646457.1	1.25e-241	92.98	hypothetical protein Csa_015524 [Cucumis sativus] >SLM22339.1 Gibberellin 9,11-d...	[more]
XP_004136071.2	1.56e-241	92.98	gibberellin 3-beta-dioxygenase 1 [Cucumis sativus]	[more]
KAA0058795.1	1.77e-201	78.51	gibberellin 3-beta-dioxygenase 1-like [Cucumis melo var. makuwa] >TYK10589.1 gib...	[more]
XP_008461418.1	2.21e-201	78.51	PREDICTED: gibberellin 3-beta-dioxygenase 1-like [Cucumis melo]	[more]
NP_001267625.1	4.39e-157	61.96	gibberellin 3-beta-dioxygenase 1-like [Cucumis sativus] >KGN45325.1 hypothetical...	[more]

BLAST of Chy1G020340 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G15550.1	6.2e-82	46.82	gibberellin 3-oxidase 1	[more]
AT1G80340.1	8.1e-82	42.57	gibberellin 3-oxidase 2	[more]
AT1G80330.1	3.0e-76	44.09	gibberellin 3-oxidase 4	[more]
AT4G21690.1	4.7e-66	40.65	gibberellin 3-oxidase 3	[more]
AT3G21420.1	1.8e-44	32.99	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 26..127 e-value: 6.2E-14 score: 52.8
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 179..276 e-value: 2.4E-22 score: 79.3
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 5..312 e-value: 2.8E-74 score: 252.4
None	No IPR available	PANTHER	PTHR47991:SF87	GIBBERELLIN 3-BETA-DIOXYGENASE 1-LIKE	coord: 17..317
None	No IPR available	PANTHER	PTHR47991	OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASE	coord: 17..317
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 13..312
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 176..276 score: 12.076986

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy1G020340.1	Chy1G020340.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009686	gibberellin biosynthetic process
biological_process	GO:0009416	response to light stimulus
molecular_function	GO:0016707	gibberellin 3-beta-dioxygenase activity
molecular_function	GO:0046872	metal ion binding