Chy1G017200 (gene) Cucumber (hystrix) v1

Overview


Name	Chy1G017200
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	Pectinesterase
Location	chrH01: 23000741 .. 23002700 (+)
RNA-Seq Expression	Chy1G017200
Synteny	Chy1G017200

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTGCCGGAACCACCGCCACCGCCGCCGCATTCTACGTCCTTCTAATCCTCATTTCACTTTACATCACCACTTCCGCCTCCCCCACCGGTTCGGTTTGTAGCTCAACTCCAGACCCATCGTATTGCAAATCGGCCCTCCCGAACCAAACCGGAAATGTGTACTCGTACGGGCGGTCGTCATTCAGAAAATCACTGTCGTCGTCACAGAAGTTCCTCCGGCTAGTGGAGAAGCACCTCCGGTCCCGATCGTCGCTGACGGTGCCGGCGGCGAGGGCGTTGGAGGACTGCCTGCTACTGGCGGGACTGAACATCGATTACTTGAAGACGTCGTTCCAGACGGTGAACGCGACGAGCAGAGCATTGACGGAGATGAAGGCGGACGATGTGCAGAGTCTGCTGAGTGCGATATTGACGAATCAACAGACTTGTTTGGACGGAATTAAGGCGACGGCGGGTTCTTGGTCGCTTAAAAATGGCCTTTCTCAACCACTTGCTTCTGACACCAAGCTGTACTCTCTGTCTTTGGCCTTCTTCACAAAAGGTTTGTCATTATTTTTTATTAAACGAATATGAGAACAAAAATTTTGAAATAAAAAGAAAAACCAGTTTTTTGTAATATTTTTATTAATTATTTGAAAGGTTGAAAATGAAAACAAGTTGTTCAAGAAAAGATACAAGTTTTCGATAATAATTACAAAATCCCTTTGAAATTTAATTAAAATAAGAGTTTAATTTTTTAAAAAAATTGTTTTTTCTCTAATCAATTTAAATATAGACTTAGTTTTTCAAAGATAATGAAACATAAAATCTTAAATATAGTTAAAATGGTAGGTTAACCAAAAGACAATGCAATTAGGTTAGGTAATTATTTTGAGTTTAATCAAATGGAGAGGTAGATTTAGTTTTCTTTCTTTTTGCTATATTTGGTTTTTATTAAATGTAATAGTAAATCAAATTATTCTATATAGAAAAAGAATCTTAGAGAGTATTTTATAAGAGATTTGGATTGAATTTTTTTGATGTGACGTTTCTTTTGTGCCTCTTTTAGGGGTGATTTGGAAAAGTGATTCGGCAGTCTATTAGAATGCCATATTCCAATGGAAAACATATATGAATAAACAAATATACATATTTAGTTCCTCTTCCTAATTCATCTGTTTTTTGTTTTTGAAAATTAATTCCTTCTAAAAAATTATTTCACAACTGATTTTTTTTTTTTAATTATGAAAGAAAAATAAAATGAAAAGCAAAACATTTTGTTTCTAAAAAATAGAAAATAAACTCAGTTACAAAACTTTTGAATAAACAAAATTAAATGAACACAAAAAACAAAACACAAACAATTAATTTCATTAAGTTATAAATTAAGAAAAGAAAAAGTAATTATTTTATTATGAGAAGTTATTGTAATAAATATTTCCGTTAACTGAACTTGAAAATATGAATTTTTTTTTTTAAATTTACGTCAGGTTGGGTTCCGAAGAAGAAGAAAAGGCCGACATGGAAAGTCGCGGGAAGACAAGGCGGGTTTAGAAACGGACGAATGTCACTCAAAATGTCCAGCAGAACCCAAGCCATTTACGAGAAGGCGACCCGCCGGAATCTGCTGCAGACTGACGACGGCGACGACGACGATCAGATTAAGGTCAGGGACATCGTCGTTGTCAGTCAAGACGGCAGCGGAAATTTCACAACCATCAACGAGGCCATTGCGGCAGCGCCCAACAACTCTGCTCCTACGGACGGGTATTTCTTGATCTTCGTGTCCGAGGGAGTTTATGAAGAGTATGTGTTAGTTGCGAAGAACAAGAGATACTTGATGATGATCGGAGATGGCATTAATCAGACCATCGTCACCGGTAATCGAAGCGTCGTTGACGGCTGGACCACTTTCAATTCCGCCACTTTTGGTAACTTCCTCCACCTTTTAGATATTGCTGAAAGAAGAGATGGATAA

mRNA sequence

ATGGCTGCCGGAACCACCGCCACCGCCGCCGCATTCTACGTCCTTCTAATCCTCATTTCACTTTACATCACCACTTCCGCCTCCCCCACCGGTTCGGTTTGTAGCTCAACTCCAGACCCATCGTATTGCAAATCGGCCCTCCCGAACCAAACCGGAAATGTGTACTCGTACGGGCGGTCGTCATTCAGAAAATCACTGTCGTCGTCACAGAAGTTCCTCCGGCTAGTGGAGAAGCACCTCCGGTCCCGATCGTCGCTGACGGTGCCGGCGGCGAGGGCGTTGGAGGACTGCCTGCTACTGGCGGGACTGAACATCGATTACTTGAAGACGTCGTTCCAGACGGTGAACGCGACGAGCAGAGCATTGACGGAGATGAAGGCGGACGATGTGCAGAGTCTGCTGAGTGCGATATTGACGAATCAACAGACTTGTTTGGACGGAATTAAGGCGACGGCGGGTTCTTGGTCGCTTAAAAATGGCCTTTCTCAACCACTTGCTTCTGACACCAAGCTGTACTCTCTGTCTTTGGCCTTCTTCACAAAAGGTTGGGTTCCGAAGAAGAAGAAAAGGCCGACATGGAAAGTCGCGGGAAGACAAGGCGGGTTTAGAAACGGACGAATGTCACTCAAAATGTCCAGCAGAACCCAAGCCATTTACGAGAAGGCGACCCGCCGGAATCTGCTGCAGACTGACGACGGCGACGACGACGATCAGATTAAGGTCAGGGACATCGTCGTTGTCAGTCAAGACGGCAGCGGAAATTTCACAACCATCAACGAGGCCATTGCGGCAGCGCCCAACAACTCTGCTCCTACGGACGGGTATTTCTTGATCTTCGTGTCCGAGGGAGTTTATGAAGAGTATGTGTTAGTTGCGAAGAACAAGAGATACTTGATGATGATCGGAGATGGCATTAATCAGACCATCGTCACCGGTAATCGAAGCGTCGTTGACGGCTGGACCACTTTCAATTCCGCCACTTTTGGTAACTTCCTCCACCTTTTAGATATTGCTGAAAGAAGAGATGGATAA

Coding sequence (CDS)

ATGGCTGCCGGAACCACCGCCACCGCCGCCGCATTCTACGTCCTTCTAATCCTCATTTCACTTTACATCACCACTTCCGCCTCCCCCACCGGTTCGGTTTGTAGCTCAACTCCAGACCCATCGTATTGCAAATCGGCCCTCCCGAACCAAACCGGAAATGTGTACTCGTACGGGCGGTCGTCATTCAGAAAATCACTGTCGTCGTCACAGAAGTTCCTCCGGCTAGTGGAGAAGCACCTCCGGTCCCGATCGTCGCTGACGGTGCCGGCGGCGAGGGCGTTGGAGGACTGCCTGCTACTGGCGGGACTGAACATCGATTACTTGAAGACGTCGTTCCAGACGGTGAACGCGACGAGCAGAGCATTGACGGAGATGAAGGCGGACGATGTGCAGAGTCTGCTGAGTGCGATATTGACGAATCAACAGACTTGTTTGGACGGAATTAAGGCGACGGCGGGTTCTTGGTCGCTTAAAAATGGCCTTTCTCAACCACTTGCTTCTGACACCAAGCTGTACTCTCTGTCTTTGGCCTTCTTCACAAAAGGTTGGGTTCCGAAGAAGAAGAAAAGGCCGACATGGAAAGTCGCGGGAAGACAAGGCGGGTTTAGAAACGGACGAATGTCACTCAAAATGTCCAGCAGAACCCAAGCCATTTACGAGAAGGCGACCCGCCGGAATCTGCTGCAGACTGACGACGGCGACGACGACGATCAGATTAAGGTCAGGGACATCGTCGTTGTCAGTCAAGACGGCAGCGGAAATTTCACAACCATCAACGAGGCCATTGCGGCAGCGCCCAACAACTCTGCTCCTACGGACGGGTATTTCTTGATCTTCGTGTCCGAGGGAGTTTATGAAGAGTATGTGTTAGTTGCGAAGAACAAGAGATACTTGATGATGATCGGAGATGGCATTAATCAGACCATCGTCACCGGTAATCGAAGCGTCGTTGACGGCTGGACCACTTTCAATTCCGCCACTTTTGGTAACTTCCTCCACCTTTTAGATATTGCTGAAAGAAGAGATGGATAA

Protein sequence

MAAGTTATAAAFYVLLILISLYITTSASPTGSVCSSTPDPSYCKSALPNQTGNVYSYGRSSFRKSLSSSQKFLRLVEKHLRSRSSLTVPAARALEDCLLLAGLNIDYLKTSFQTVNATSRALTEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAFFTKGWVPKKKKRPTWKVAGRQGGFRNGRMSLKMSSRTQAIYEKATRRNLLQTDDGDDDDQIKVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSEGVYEEYVLVAKNKRYLMMIGDGINQTIVTGNRSVVDGWTTFNSATFGNFLHLLDIAERRDG*

Homology

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BLAST of Chy1G017200 vs. ExPASy Swiss-Prot
Match: Q9SRX4 (Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PME7 PE=2 SV=1)

HSP 1 Score: 336.7 bits (862), Expect = 3.2e-91
Identity = 181/340 (53.24%), Postives = 241/340 (70.88%), Query Frame = 0


Query: 11  AFYVLLILISLYITTSASPTGSVCSSTPDPSYCKSALPNQTGNVYSYGRSSFRKSLSSSQ 70
           +F++  +L S   + + S + ++C +TPDP YCKS  P+  GNV  YG  S RKSLS S+
Sbjct: 12  SFFLQSVLAS---SQTLSNSSTICKTTPDPKYCKSVFPHSQGNVQQYGCFSIRKSLSQSR 71

Query: 71  KFLRLVEKHLRSRSSLTVPAA-RALEDCLLLAGLNIDYLKTSFQTVNATS-----RALTE 130
           KF+R V+++++  + L+ PA  RAL+DC  LAGL +DYL TSF+TVN TS     + L+ 
Sbjct: 72  KFIRTVDRYIKRNAHLSQPAVIRALQDCRFLAGLTMDYLLTSFETVNDTSAKTSFKPLSF 131

Query: 131 MKADDVQSLLSAILTNQQTCLDGIKATA---GSWSLKNGLSQPLASDTKLYSLSLAFFTK 190
            KADD+Q+LLSA LTN+QTCL+G+   A    +W+++ G++ PL +DTKL  +SLA FTK
Sbjct: 132 PKADDIQTLLSAALTNEQTCLEGLTTAASYSATWTVRTGVALPLVNDTKLLGVSLALFTK 191

Query: 191 GWVPKKKKRPTWKVAGRQGG----------FRNGRMSLKMSSRTQAIYEKATRRNLLQTD 250
           GWVPKKKKR  +  A  + G          FRNG + LKM+ +T+A+YE  +RR L   D
Sbjct: 192 GWVPKKKKRAGFAWAQPRSGSSTHTKPFRLFRNGALPLKMTEKTKAVYESLSRRKLADGD 251

Query: 251 ---DGDDDDQIKVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSEGVYEEY 310
              DGDD   + + DIV VSQDG+GNFT I  A+AAAPNN+  + G+FLI+V+ G+YEEY
Sbjct: 252 SNGDGDDGSMVLISDIVTVSQDGTGNFTNITAAVAAAPNNTDGSAGFFLIYVTAGIYEEY 311

Query: 311 VLVAKNKRYLMMIGDGINQTIVTGNRSVVDGWTTFNSATF 329
           + +AKNKRY+MMIGDGINQT+VTGNRSVVDGWTTFNSATF
Sbjct: 312 ISIAKNKRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATF 348

[Close]

BLAST of Chy1G017200 vs. ExPASy Swiss-Prot
Match: O22256 (Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana OX=3702 GN=PME20 PE=2 SV=2)

HSP 1 Score: 317.4 bits (812), Expect = 2.0e-85
Identity = 167/308 (54.22%), Postives = 227/308 (73.70%), Query Frame = 0


Query: 26  SASPTGSVCSSTPDPSYCKSALPNQTGNVYSYGRSSFRKSLSSSQKFLRLVEKHLRSRSS 85
           +A+   ++C   PDPSYC+S LPNQ G++YSYGR S R+SLS +++F+ +++  L  +  
Sbjct: 31  NATSPSNICRFAPDPSYCRSVLPNQPGDIYSYGRLSLRRSLSRARRFISMIDAELDRKGK 90

Query: 86  LTVPA-ARALEDCLLLAGLNIDYLKTSFQTVNATSRALTEMKADDVQSLLSAILTNQQTC 145
           +   +   ALEDC  LA L +DYL +S QT ++T + L+  +A+DV + LSA +TN+QTC
Sbjct: 91  VAAKSTVGALEDCKFLASLTMDYLLSSSQTADST-KTLSLSRAEDVHTFLSAAITNEQTC 150

Query: 146 LDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAFFTKGWVPKKKK-RPTWKVAGRQG--- 205
           L+G+K+TA     +NGLS  L +DTKLY +SLA F+KGWVP++++ RP W+   R     
Sbjct: 151 LEGLKSTAS----ENGLSGDLFNDTKLYGVSLALFSKGWVPRRQRSRPIWQPQARFKKFF 210

Query: 206 GFRNGRMSLKMSSRTQAIYEKATRRNLLQTDDGDDDDQIKVRDIVVVSQDGSGNFTTINE 265
           GFRNG++ LKM+ R +A+Y   TRR LLQ+    D D ++V DIV V Q+G+GNFTTIN 
Sbjct: 211 GFRNGKLPLKMTERARAVYNTVTRRKLLQS----DADAVQVSDIVTVIQNGTGNFTTINA 270

Query: 266 AIAAAPNNSAPTDGYFLIFVSEGVYEEYVLVAKNKRYLMMIGDGINQTIVTGNRSVVDGW 325
           AIAAAPN +  ++GYFLI+V+ G+YEEYV V KNKRY+MMIGDGINQT++TGNRSVVDGW
Sbjct: 271 AIAAAPNKTDGSNGYFLIYVTAGLYEEYVEVPKNKRYVMMIGDGINQTVITGNRSVVDGW 329

Query: 326 TTFNSATF 329
           TTFNSATF
Sbjct: 331 TTFNSATF 329

[Close]

BLAST of Chy1G017200 vs. ExPASy Swiss-Prot
Match: Q8RXK7 (Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3702 GN=PME41 PE=2 SV=2)

HSP 1 Score: 315.5 bits (807), Expect = 7.6e-85
Identity = 176/334 (52.69%), Postives = 239/334 (71.56%), Query Frame = 0


Query: 14  VLLILISLYITTS----ASPTGSVCSSTPDPSYCKSALPNQT-GNVYSYGRSSFRKSLSS 73
           + L L +L+I +     ++ + ++C +TPDP +CKS  P  + G+V  YGR S RKSL+ 
Sbjct: 11  LFLSLQTLFIASQTLLPSNSSSTICKTTPDPKFCKSVFPQTSQGDVREYGRFSLRKSLTQ 70

Query: 74  SQKFLRLVEKHL-RSRSSLTVPAARALEDCLLLAGLNIDYLKTSFQTVNAT--SRALTEM 133
           S+KF R ++++L R+ + L+  A  AL+DC  LA L  DYL TSF+TVN T  S+ L+  
Sbjct: 71  SRKFTRTIDRYLKRNNALLSQSAVGALQDCRYLASLTTDYLITSFETVNITTSSKTLSFS 130

Query: 134 KADDVQSLLSAILTNQQTCLDGIK-ATAGSWSLKNGLSQPLASDTKLYSLSLAFFTKGWV 193
           KAD++Q+LLSA LTN+QTCLDGI  A + SW+++NG++ PL +DTKL+S+SLA FTKGWV
Sbjct: 131 KADEIQTLLSAALTNEQTCLDGINTAASSSWTIRNGVALPLINDTKLFSVSLALFTKGWV 190

Query: 194 PKKKKRP---TWKVAGRQGG-------FRNGRMSLKMSSRTQAIYEKATRRNLLQTDDGD 253
           PKKKK+    +W               FRNG + LKM+  T+A+YE  +RR L   DD +
Sbjct: 191 PKKKKQVASYSWAHPKNTHSHTKPFRHFRNGALPLKMTEHTRAVYESLSRRKL--ADDDN 250

Query: 254 DDDQIKVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSEGVYEEYVLVAKN 313
           D + + V DIV V+Q+G+GNFTTI EA+ +APN +  T GYF+I+V+ GVYEE V++AKN
Sbjct: 251 DVNTVLVSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIYVTSGVYEENVVIAKN 310

Query: 314 KRYLMMIGDGINQTIVTGNRSVVDGWTTFNSATF 329
           KRYLMMIGDGIN+T+VTGNR+VVDGWTTFNSATF
Sbjct: 311 KRYLMMIGDGINRTVVTGNRNVVDGWTTFNSATF 342

[Close]

BLAST of Chy1G017200 vs. ExPASy Swiss-Prot
Match: Q94CB1 (Probable pectinesterase/pectinesterase inhibitor 25 OS=Arabidopsis thaliana OX=3702 GN=PME25 PE=2 SV=1)

HSP 1 Score: 171.8 bits (434), Expect = 1.4e-41
Identity = 117/322 (36.34%), Postives = 178/322 (55.28%), Query Frame = 0


Query: 20  SLYITTSASPTGSVCSSTPDPSYCK---SALPNQTGNVYSYGRSSFRKSLSSSQKFLRLV 79
           SL  + S SP+   C STP P  C+   +A+ +   + Y YG+ + ++ L  + +  +++
Sbjct: 70  SLPPSQSQSPS-LACKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQASRLSKVI 129

Query: 80  EKHLR----SRSSLTVPAARALEDCLLLAGLNIDYLKTSFQTVNATSRALTEMKADDVQS 139
             + R       S T     A+ DC  L+ L+++YL+T   T   T++ +T    + V S
Sbjct: 130 TSYARRVESKPGSATAEEIGAVADCGELSELSVNYLET-VTTELKTAQVMTAALVEHVNS 189

Query: 140 LLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAFFTKGWVPKKKKRPT 199
           LLS ++TNQQTCLDG+      ++    +  P+ + T+LYS+SL   +       K+   
Sbjct: 190 LLSGVVTNQQTCLDGLVEAKSGFAA--AIGSPMGNLTRLYSISLGLVSHALNRNLKRFKA 249

Query: 200 WK---VAGRQGGFRNGRMSLKMSSRTQAIYEK---ATRRNLLQTDDGDDDDQIKVRDIVV 259
            K   + G    +R    +L    R     +K    T RNL +  +      I V   V+
Sbjct: 250 SKGKILGGGNSTYREPLETLIKGLRKTCDNDKDCRKTSRNLGELGE-TSGGSILVSKAVI 309

Query: 260 VSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSEGVYEEYVLVAKNKRYLMMIGDGIN 319
           V    S NFTTI +AIAAAPNN+ P DGYF+I+  EGVYEEY++V  NK+ LM++GDGIN
Sbjct: 310 VGPFKSDNFTTITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMGDGIN 369

Query: 320 QTIVTGNRSVVDGWTTFNSATF 329
           +TI+TGN +VVDGWTT+N ++F
Sbjct: 370 KTIITGNHNVVDGWTTYNCSSF 386

[Close]

BLAST of Chy1G017200 vs. ExPASy Swiss-Prot
Match: Q9FF77 (Probable pectinesterase/pectinesterase inhibitor 47 OS=Arabidopsis thaliana OX=3702 GN=PME47 PE=2 SV=1)

HSP 1 Score: 164.9 bits (416), Expect = 1.7e-39
Identity = 111/321 (34.58%), Postives = 172/321 (53.58%), Query Frame = 0


Query: 26  SASPTGSVCSSTPDPSYCK---SALPNQTGNVYSYGRSSFRKSLSSSQKFLRLVEKHLR- 85
           S  P    C STP P  C+   SA+ +   + Y YG+ + ++ L  +++  +++ +  + 
Sbjct: 80  SPLPPNIACKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQARRLSKVINRFAQR 139

Query: 86  ---SRSSLTVPAARALEDCLLLAGLNIDYLKTSFQTVNATSRALTEMKADDVQSLLSAIL 145
                 + TV    A+ DC  LA L+++YL+T  + + A +  +T    D V SLL  ++
Sbjct: 140 VEADPGTSTVEEVSAVADCGELAELSVEYLETVTEELKA-AELMTAALVDRVTSLLGGVV 199

Query: 146 TNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAFFTKGWVPKKKKRPTWKVAGR 205
           TNQQTCLDG+      ++    +  PL + T+LYS+SL     G V     R   +  G 
Sbjct: 200 TNQQTCLDGLVDAKSGFA--TAIGTPLGNLTRLYSVSL-----GLVSHALNRNLKRYKGS 259

Query: 206 QGG-FRNGRMSLKMSSRTQAIYEKAT----------RRNLLQTDDGDDDDQIKVRDIVVV 265
           +G  F  G   ++    T     + T           RNL +  +      I VR+ V V
Sbjct: 260 KGKIFGGGNKPVREPLETLIKVLRKTCDKGKDCRKANRNLGELGE-TSGGSILVREAVTV 319

Query: 266 SQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSEGVYEEYVLVAKNKRYLMMIGDGINQ 325
               + NF TI EA+AAAPN++ P  GYF+I+   G+YEEYV+++  KR +M+IGDGIN+
Sbjct: 320 GPYETDNFPTITEAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKKRNIMLIGDGINK 379

Query: 326 TIVTGNRSVVDGWTTFNSATF 329
           TI++GN S +DGWTT+NS+TF
Sbjct: 380 TIISGNHSFIDGWTTYNSSTF 391

[Close]

BLAST of Chy1G017200 vs. ExPASy TrEMBL
Match: A0A0A0KB12 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_7G405820 PE=3 SV=1)

HSP 1 Score: 612.5 bits (1578), Expect = 1.1e-171
Identity = 321/328 (97.87%), Postives = 321/328 (97.87%), Query Frame = 0


Query: 1   MAAGTTATAAAFYVLLILISLYITTSASPTGSVCSSTPDPSYCKSALPNQTGNVYSYGRS 60
           MAAGTTATAAAFYVLLILISLYITTSASPTGSVCSSTPDPSYCKSALPNQTGNVYSYGRS
Sbjct: 1   MAAGTTATAAAFYVLLILISLYITTSASPTGSVCSSTPDPSYCKSALPNQTGNVYSYGRS 60

Query: 61  SFRKSLSSSQKFLRLVEKHLRSRSSLTVPAARALEDCLLLAGLNIDYLKTSFQTVNATSR 120
           SFRKSLSSSQKFLRLVEKHLRSRSSLTVPA RALEDCLLLAGLNIDYLKTSFQTVN TSR
Sbjct: 61  SFRKSLSSSQKFLRLVEKHLRSRSSLTVPAVRALEDCLLLAGLNIDYLKTSFQTVNTTSR 120

Query: 121 ALTEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAFFT 180
            LTEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAFFT
Sbjct: 121 VLTEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAFFT 180

Query: 181 KGWVPKKKKRPTWKVAGRQGGFRNGRMSLKMSSRTQAIYEKATRRNLLQTDDGDDDDQIK 240
           KGWVPKKKKRPTWK AGRQGGFRNGRMSLKMSSRTQAIYEKATRRNLLQTDDG DDDQIK
Sbjct: 181 KGWVPKKKKRPTWKAAGRQGGFRNGRMSLKMSSRTQAIYEKATRRNLLQTDDGGDDDQIK 240

Query: 241 VRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSEGVYEEYVLVAKNKRYLMM 300
           VRDIVVVSQDGSGNFTTINEAIAAA NNSAPTDGYFLIFVS GVYEEYVLVAKNKRYLMM
Sbjct: 241 VRDIVVVSQDGSGNFTTINEAIAAATNNSAPTDGYFLIFVSAGVYEEYVLVAKNKRYLMM 300

Query: 301 IGDGINQTIVTGNRSVVDGWTTFNSATF 329
           IGDGINQTIVTGNRSVVDGWTTFNSATF
Sbjct: 301 IGDGINQTIVTGNRSVVDGWTTFNSATF 328

[Close]

BLAST of Chy1G017200 vs. ExPASy TrEMBL
Match: A0A5D3D3H4 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G001320 PE=3 SV=1)

HSP 1 Score: 582.0 bits (1499), Expect = 1.6e-162
Identity = 311/329 (94.53%), Postives = 315/329 (95.74%), Query Frame = 0


Query: 1   MAAGTTATAAAFYVLLILISLYITTSASPTGSVCSSTPDPSYCKSALPNQTGNVYSYGRS 60
           MAAGT  TAAAFYV LILISLYITTSASPTGSVCSSTPDPSYCKSALPNQTGNVYSYGRS
Sbjct: 1   MAAGT--TAAAFYV-LILISLYITTSASPTGSVCSSTPDPSYCKSALPNQTGNVYSYGRS 60

Query: 61  SFRKSLSSSQKFLRLVEKHLRSRSSLTVPAARALEDCLLLAGLNIDYLKTSFQTVNATSR 120
           SFRKSLSSSQKFLRLVEKHLRSRSSLTV A RALEDC LLAGLNIDYL+TSFQ VNATSR
Sbjct: 61  SFRKSLSSSQKFLRLVEKHLRSRSSLTVAAVRALEDCQLLAGLNIDYLRTSFQAVNATSR 120

Query: 121 ALTEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAFFT 180
           ALTEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAFFT
Sbjct: 121 ALTEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAFFT 180

Query: 181 KGWVPKKKKRPTWKVAGRQGGFRNGRMSLKMSSRTQAIYEKATRRNLLQT-DDGDDDDQI 240
           KGWVPKKKK+PTWK AGRQGGFRNGRMSLKMSSRTQAIYE ATRRNLLQT DDG DDDQI
Sbjct: 181 KGWVPKKKKKPTWKAAGRQGGFRNGRMSLKMSSRTQAIYETATRRNLLQTDDDGGDDDQI 240

Query: 241 KVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSEGVYEEYVLVAKNKRYLM 300
            VRDIVVVSQDG+GNFTTINEAIAAAPNNSAPTDGYFLIFVS GVYEEYV +AKNKRYLM
Sbjct: 241 AVRDIVVVSQDGTGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVSIAKNKRYLM 300

Query: 301 MIGDGINQTIVTGNRSVVDGWTTFNSATF 329
           MIGDGINQTIVTGNRSVVDGWTTFNSATF
Sbjct: 301 MIGDGINQTIVTGNRSVVDGWTTFNSATF 326

[Close]

BLAST of Chy1G017200 vs. ExPASy TrEMBL
Match: A0A1S3BSI2 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103492791 PE=3 SV=1)

HSP 1 Score: 582.0 bits (1499), Expect = 1.6e-162
Identity = 311/329 (94.53%), Postives = 315/329 (95.74%), Query Frame = 0


Query: 1   MAAGTTATAAAFYVLLILISLYITTSASPTGSVCSSTPDPSYCKSALPNQTGNVYSYGRS 60
           MAAGT  TAAAFYV LILISLYITTSASPTGSVCSSTPDPSYCKSALPNQTGNVYSYGRS
Sbjct: 1   MAAGT--TAAAFYV-LILISLYITTSASPTGSVCSSTPDPSYCKSALPNQTGNVYSYGRS 60

Query: 61  SFRKSLSSSQKFLRLVEKHLRSRSSLTVPAARALEDCLLLAGLNIDYLKTSFQTVNATSR 120
           SFRKSLSSSQKFLRLVEKHLRSRSSLTV A RALEDC LLAGLNIDYL+TSFQ VNATSR
Sbjct: 61  SFRKSLSSSQKFLRLVEKHLRSRSSLTVAAVRALEDCQLLAGLNIDYLRTSFQAVNATSR 120

Query: 121 ALTEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAFFT 180
           ALTEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAFFT
Sbjct: 121 ALTEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAFFT 180

Query: 181 KGWVPKKKKRPTWKVAGRQGGFRNGRMSLKMSSRTQAIYEKATRRNLLQT-DDGDDDDQI 240
           KGWVPKKKK+PTWK AGRQGGFRNGRMSLKMSSRTQAIYE ATRRNLLQT DDG DDDQI
Sbjct: 181 KGWVPKKKKKPTWKAAGRQGGFRNGRMSLKMSSRTQAIYETATRRNLLQTDDDGGDDDQI 240

Query: 241 KVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSEGVYEEYVLVAKNKRYLM 300
            VRDIVVVSQDG+GNFTTINEAIAAAPNNSAPTDGYFLIFVS GVYEEYV +AKNKRYLM
Sbjct: 241 AVRDIVVVSQDGTGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVSIAKNKRYLM 300

Query: 301 MIGDGINQTIVTGNRSVVDGWTTFNSATF 329
           MIGDGINQTIVTGNRSVVDGWTTFNSATF
Sbjct: 301 MIGDGINQTIVTGNRSVVDGWTTFNSATF 326

[Close]

BLAST of Chy1G017200 vs. ExPASy TrEMBL
Match: A0A6J1GQE6 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111456155 PE=3 SV=1)

HSP 1 Score: 533.5 bits (1373), Expect = 6.6e-148
Identity = 285/331 (86.10%), Postives = 299/331 (90.33%), Query Frame = 0


Query: 3   AGTTATAAAFYVLLILISLYIT-----TSASPTGSVCSSTPDPSYCKSALPNQTGNVYSY 62
           A    T AAFY LL LISLYIT     TSA+PTGSVCSS PDPSYCKSALPNQTGNVYSY
Sbjct: 2   AAAPPTTAAFYSLL-LISLYITAAGISTSAAPTGSVCSSAPDPSYCKSALPNQTGNVYSY 61

Query: 63  GRSSFRKSLSSSQKFLRLVEKHLRSRSSLTVPAARALEDCLLLAGLNIDYLKTSFQTVNA 122
           GRSS RKSLSSSQKFLRLVEK+LRSRSSLTV A RALEDC LLAGLNIDYL TSF+TVN+
Sbjct: 62  GRSSVRKSLSSSQKFLRLVEKYLRSRSSLTVAAVRALEDCQLLAGLNIDYLTTSFRTVNS 121

Query: 123 TSRALTEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLA 182
           TS  L EMKA+DVQSLLSAILTNQQTCLDG+KATAGSWSLKNGLS PLA+DTKLYS+SLA
Sbjct: 122 TSWTLPEMKAEDVQSLLSAILTNQQTCLDGLKATAGSWSLKNGLSLPLAADTKLYSMSLA 181

Query: 183 FFTKGWVPKKKKRPTWKVAGRQGGFRNGRMSLKMSSRTQAIYEKATRRNLLQTDDGDDDD 242
           FFTKGWVPKKKK+PTW  AGRQGGF NGRMSLKMSSRT+AIYE ATRRNLLQT+DG  DD
Sbjct: 182 FFTKGWVPKKKKKPTWTSAGRQGGFNNGRMSLKMSSRTKAIYETATRRNLLQTEDG-GDD 241

Query: 243 QIKVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSEGVYEEYVLVAKNKRY 302
           QI VRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFV  GVYEEYV +AKNKRY
Sbjct: 242 QISVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVDAGVYEEYVTIAKNKRY 301

Query: 303 LMMIGDGINQTIVTGNRSVVDGWTTFNSATF 329
           LMMIGDGINQT+VTGNR+VVDGWTTFNSATF
Sbjct: 302 LMMIGDGINQTVVTGNRNVVDGWTTFNSATF 330

[Close]

BLAST of Chy1G017200 vs. ExPASy TrEMBL
Match: A0A6J1JVJ5 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111487941 PE=3 SV=1)

HSP 1 Score: 525.4 bits (1352), Expect = 1.8e-145
Identity = 281/333 (84.38%), Postives = 297/333 (89.19%), Query Frame = 0


Query: 1   MAAGTTATAAAFYVLLILISLYIT-----TSASPTGSVCSSTPDPSYCKSALPNQTGNVY 60
           MAA    T A +Y  L+LISLYIT     TSA+PTGSVCSS PDPSYCKSALPNQTGNVY
Sbjct: 1   MAAAPPTTPAFYY--LLLISLYITAAGISTSAAPTGSVCSSAPDPSYCKSALPNQTGNVY 60

Query: 61  SYGRSSFRKSLSSSQKFLRLVEKHLRSRSSLTVPAARALEDCLLLAGLNIDYLKTSFQTV 120
           SYGRSS RKSLSSSQKFLRLVEK+LRSRSSLTV A R+LEDC LLAGLNIDYL TSF+TV
Sbjct: 61  SYGRSSVRKSLSSSQKFLRLVEKYLRSRSSLTVAAVRSLEDCQLLAGLNIDYLTTSFRTV 120

Query: 121 NATSRALTEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLS 180
           N+TS  L EMKA+DVQSLLSAILTNQQTCLDG+KATAGSWSLKNGLS PL +DTKLYS+S
Sbjct: 121 NSTSWTLPEMKAEDVQSLLSAILTNQQTCLDGLKATAGSWSLKNGLSLPLDADTKLYSMS 180

Query: 181 LAFFTKGWVPKKKKRPTWKVAGRQGGFRNGRMSLKMSSRTQAIYEKATRRNLLQTDDGDD 240
           LAFFTKGWVPKKKK+ TW  AGRQGGF NGRMS KMSSRT+AIYE ATRRNLLQTDDG  
Sbjct: 181 LAFFTKGWVPKKKKKQTWTSAGRQGGFSNGRMSFKMSSRTKAIYEMATRRNLLQTDDG-G 240

Query: 241 DDQIKVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSEGVYEEYVLVAKNK 300
           DDQI VRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFV  GVYEEYV +AKNK
Sbjct: 241 DDQISVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVDAGVYEEYVTIAKNK 300

Query: 301 RYLMMIGDGINQTIVTGNRSVVDGWTTFNSATF 329
           RYLMMIGDGINQT+VTGNR+VVDGWTTFNSATF
Sbjct: 301 RYLMMIGDGINQTVVTGNRNVVDGWTTFNSATF 330

[Close]

BLAST of Chy1G017200 vs. NCBI nr
Match: XP_004136013.1 (probable pectinesterase/pectinesterase inhibitor 7 [Cucumis sativus] >KGN44976.1 hypothetical protein Csa_016133 [Cucumis sativus])

HSP 1 Score: 615 bits (1585), Expect = 2.60e-217
Identity = 321/328 (97.87%), Postives = 321/328 (97.87%), Query Frame = 0


Query: 1   MAAGTTATAAAFYVLLILISLYITTSASPTGSVCSSTPDPSYCKSALPNQTGNVYSYGRS 60
           MAAGTTATAAAFYVLLILISLYITTSASPTGSVCSSTPDPSYCKSALPNQTGNVYSYGRS
Sbjct: 1   MAAGTTATAAAFYVLLILISLYITTSASPTGSVCSSTPDPSYCKSALPNQTGNVYSYGRS 60

Query: 61  SFRKSLSSSQKFLRLVEKHLRSRSSLTVPAARALEDCLLLAGLNIDYLKTSFQTVNATSR 120
           SFRKSLSSSQKFLRLVEKHLRSRSSLTVPA RALEDCLLLAGLNIDYLKTSFQTVN TSR
Sbjct: 61  SFRKSLSSSQKFLRLVEKHLRSRSSLTVPAVRALEDCLLLAGLNIDYLKTSFQTVNTTSR 120

Query: 121 ALTEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAFFT 180
            LTEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAFFT
Sbjct: 121 VLTEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAFFT 180

Query: 181 KGWVPKKKKRPTWKVAGRQGGFRNGRMSLKMSSRTQAIYEKATRRNLLQTDDGDDDDQIK 240
           KGWVPKKKKRPTWK AGRQGGFRNGRMSLKMSSRTQAIYEKATRRNLLQTDDG DDDQIK
Sbjct: 181 KGWVPKKKKRPTWKAAGRQGGFRNGRMSLKMSSRTQAIYEKATRRNLLQTDDGGDDDQIK 240

Query: 241 VRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSEGVYEEYVLVAKNKRYLMM 300
           VRDIVVVSQDGSGNFTTINEAIAAA NNSAPTDGYFLIFVS GVYEEYVLVAKNKRYLMM
Sbjct: 241 VRDIVVVSQDGSGNFTTINEAIAAATNNSAPTDGYFLIFVSAGVYEEYVLVAKNKRYLMM 300

Query: 301 IGDGINQTIVTGNRSVVDGWTTFNSATF 328
           IGDGINQTIVTGNRSVVDGWTTFNSATF
Sbjct: 301 IGDGINQTIVTGNRSVVDGWTTFNSATF 328

[Close]

BLAST of Chy1G017200 vs. NCBI nr
Match: XP_008451544.1 (PREDICTED: probable pectinesterase/pectinesterase inhibitor 7 [Cucumis melo] >KAA0046659.1 putative pectinesterase/pectinesterase inhibitor 7 [Cucumis melo var. makuwa] >TYK18194.1 putative pectinesterase/pectinesterase inhibitor 7 [Cucumis melo var. makuwa])

HSP 1 Score: 585 bits (1507), Expect = 1.73e-205
Identity = 311/329 (94.53%), Postives = 315/329 (95.74%), Query Frame = 0


Query: 1   MAAGTTATAAAFYVLLILISLYITTSASPTGSVCSSTPDPSYCKSALPNQTGNVYSYGRS 60
           MAAGTTA  AAFYVL ILISLYITTSASPTGSVCSSTPDPSYCKSALPNQTGNVYSYGRS
Sbjct: 1   MAAGTTA--AAFYVL-ILISLYITTSASPTGSVCSSTPDPSYCKSALPNQTGNVYSYGRS 60

Query: 61  SFRKSLSSSQKFLRLVEKHLRSRSSLTVPAARALEDCLLLAGLNIDYLKTSFQTVNATSR 120
           SFRKSLSSSQKFLRLVEKHLRSRSSLTV A RALEDC LLAGLNIDYL+TSFQ VNATSR
Sbjct: 61  SFRKSLSSSQKFLRLVEKHLRSRSSLTVAAVRALEDCQLLAGLNIDYLRTSFQAVNATSR 120

Query: 121 ALTEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAFFT 180
           ALTEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAFFT
Sbjct: 121 ALTEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAFFT 180

Query: 181 KGWVPKKKKRPTWKVAGRQGGFRNGRMSLKMSSRTQAIYEKATRRNLLQTDD-GDDDDQI 240
           KGWVPKKKK+PTWK AGRQGGFRNGRMSLKMSSRTQAIYE ATRRNLLQTDD G DDDQI
Sbjct: 181 KGWVPKKKKKPTWKAAGRQGGFRNGRMSLKMSSRTQAIYETATRRNLLQTDDDGGDDDQI 240

Query: 241 KVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSEGVYEEYVLVAKNKRYLM 300
            VRDIVVVSQDG+GNFTTINEAIAAAPNNSAPTDGYFLIFVS GVYEEYV +AKNKRYLM
Sbjct: 241 AVRDIVVVSQDGTGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVSIAKNKRYLM 300

Query: 301 MIGDGINQTIVTGNRSVVDGWTTFNSATF 328
           MIGDGINQTIVTGNRSVVDGWTTFNSATF
Sbjct: 301 MIGDGINQTIVTGNRSVVDGWTTFNSATF 326

[Close]

BLAST of Chy1G017200 vs. NCBI nr
Match: XP_038896831.1 (probable pectinesterase/pectinesterase inhibitor 7 [Benincasa hispida])

HSP 1 Score: 556 bits (1433), Expect = 3.33e-194
Identity = 293/330 (88.79%), Postives = 304/330 (92.12%), Query Frame = 0


Query: 1   MAAGT--TATAAAFYVLLILISLYITTSASPTGSVCSSTPDPSYCKSALPNQTGNVYSYG 60
           MAAGT   ATAAA +  LILISLYITTSA+PTGS CSST DPSYCKS LPNQTGNVYSYG
Sbjct: 1   MAAGTIPAATAAAAFYFLILISLYITTSAAPTGSECSSTRDPSYCKSVLPNQTGNVYSYG 60

Query: 61  RSSFRKSLSSSQKFLRLVEKHLRSRSSLTVPAARALEDCLLLAGLNIDYLKTSFQTVNAT 120
           RSSFRKSLS+SQKFLRLVEKHLRSRSSLTVPA RALEDC LLAGLNIDYL TSF+ VN+T
Sbjct: 61  RSSFRKSLSASQKFLRLVEKHLRSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRAVNST 120

Query: 121 SRALTEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAF 180
           SR L EMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLS PLA+DTKLYSLSLAF
Sbjct: 121 SRTLPEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSLPLAADTKLYSLSLAF 180

Query: 181 FTKGWVPKKKKRPTWKVAGRQGGFRNGRMSLKMSSRTQAIYEKATRRNLLQTDDGDDDDQ 240
           FTKGWVPKKKK+PTWK AGR GGFRNGRMSLKMSSR +AIYE ATRRNLLQ DDG DD Q
Sbjct: 181 FTKGWVPKKKKKPTWKAAGRHGGFRNGRMSLKMSSRAKAIYETATRRNLLQADDGGDD-Q 240

Query: 241 IKVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSEGVYEEYVLVAKNKRYL 300
           I VRDIVVVSQDGSGNFTTINEA+AAAPNNSAPTDGYFLIFVS GVYEEYV +AKNKRYL
Sbjct: 241 IAVRDIVVVSQDGSGNFTTINEAVAAAPNNSAPTDGYFLIFVSAGVYEEYVSIAKNKRYL 300

Query: 301 MMIGDGINQTIVTGNRSVVDGWTTFNSATF 328
           MMIGDGINQT+VTGNRSVVDGWTTFNSATF
Sbjct: 301 MMIGDGINQTVVTGNRSVVDGWTTFNSATF 329

[Close]

BLAST of Chy1G017200 vs. NCBI nr
Match: KAG7014346.1 (putative pectinesterase/pectinesterase inhibitor 41, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 538 bits (1385), Expect = 6.66e-187
Identity = 285/326 (87.42%), Postives = 298/326 (91.41%), Query Frame = 0


Query: 8   TAAAFYVLLILISLYIT-----TSASPTGSVCSSTPDPSYCKSALPNQTGNVYSYGRSSF 67
           T AAFY LL LISLYIT     TSA+PTGSVCSS PDPSYCKSALPNQTGNVYSYGRSS 
Sbjct: 7   TTAAFYSLL-LISLYITAAGISTSAAPTGSVCSSAPDPSYCKSALPNQTGNVYSYGRSSV 66

Query: 68  RKSLSSSQKFLRLVEKHLRSRSSLTVPAARALEDCLLLAGLNIDYLKTSFQTVNATSRAL 127
           RKSLSSSQKFLRLVEK+LRSRSSLTV A RALEDC LLAGLNIDYL TSF+TVN+TS  L
Sbjct: 67  RKSLSSSQKFLRLVEKYLRSRSSLTVAAVRALEDCQLLAGLNIDYLTTSFRTVNSTSWTL 126

Query: 128 TEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAFFTKG 187
            EMKA+DVQSLLSAILTNQQTCLDG+KATAGSWSLKNGLS PLA+DTKLYS+SLAFFTKG
Sbjct: 127 PEMKAEDVQSLLSAILTNQQTCLDGLKATAGSWSLKNGLSLPLAADTKLYSMSLAFFTKG 186

Query: 188 WVPKKKKRPTWKVAGRQGGFRNGRMSLKMSSRTQAIYEKATRRNLLQTDDGDDDDQIKVR 247
           WVPKKKK+PTW  AGRQGGF NGRMSLKMSSRT+AIYE ATRRNLLQTDDG DD QI VR
Sbjct: 187 WVPKKKKKPTWTSAGRQGGFSNGRMSLKMSSRTKAIYETATRRNLLQTDDGGDD-QISVR 246

Query: 248 DIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSEGVYEEYVLVAKNKRYLMMIG 307
           DIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFV  GVYEEYV +AKNKRYLMMIG
Sbjct: 247 DIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVDAGVYEEYVTIAKNKRYLMMIG 306

Query: 308 DGINQTIVTGNRSVVDGWTTFNSATF 328
           DGINQT+VTGNR+VVDGWTTFNSATF
Sbjct: 307 DGINQTVVTGNRNVVDGWTTFNSATF 330

[Close]

BLAST of Chy1G017200 vs. NCBI nr
Match: XP_022953705.1 (probable pectinesterase/pectinesterase inhibitor 7 [Cucurbita moschata])

HSP 1 Score: 536 bits (1382), Expect = 1.90e-186
Identity = 284/326 (87.12%), Postives = 298/326 (91.41%), Query Frame = 0


Query: 8   TAAAFYVLLILISLYIT-----TSASPTGSVCSSTPDPSYCKSALPNQTGNVYSYGRSSF 67
           T AAFY LL LISLYIT     TSA+PTGSVCSS PDPSYCKSALPNQTGNVYSYGRSS 
Sbjct: 7   TTAAFYSLL-LISLYITAAGISTSAAPTGSVCSSAPDPSYCKSALPNQTGNVYSYGRSSV 66

Query: 68  RKSLSSSQKFLRLVEKHLRSRSSLTVPAARALEDCLLLAGLNIDYLKTSFQTVNATSRAL 127
           RKSLSSSQKFLRLVEK+LRSRSSLTV A RALEDC LLAGLNIDYL TSF+TVN+TS  L
Sbjct: 67  RKSLSSSQKFLRLVEKYLRSRSSLTVAAVRALEDCQLLAGLNIDYLTTSFRTVNSTSWTL 126

Query: 128 TEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAFFTKG 187
            EMKA+DVQSLLSAILTNQQTCLDG+KATAGSWSLKNGLS PLA+DTKLYS+SLAFFTKG
Sbjct: 127 PEMKAEDVQSLLSAILTNQQTCLDGLKATAGSWSLKNGLSLPLAADTKLYSMSLAFFTKG 186

Query: 188 WVPKKKKRPTWKVAGRQGGFRNGRMSLKMSSRTQAIYEKATRRNLLQTDDGDDDDQIKVR 247
           WVPKKKK+PTW  AGRQGGF NGRMSLKMSSRT+AIYE ATRRNLLQT+DG DD QI VR
Sbjct: 187 WVPKKKKKPTWTSAGRQGGFNNGRMSLKMSSRTKAIYETATRRNLLQTEDGGDD-QISVR 246

Query: 248 DIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSEGVYEEYVLVAKNKRYLMMIG 307
           DIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFV  GVYEEYV +AKNKRYLMMIG
Sbjct: 247 DIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVDAGVYEEYVTIAKNKRYLMMIG 306

Query: 308 DGINQTIVTGNRSVVDGWTTFNSATF 328
           DGINQT+VTGNR+VVDGWTTFNSATF
Sbjct: 307 DGINQTVVTGNRNVVDGWTTFNSATF 330

[Close]

BLAST of Chy1G017200 vs. TAIR 10
Match: AT1G02810.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 336.7 bits (862), Expect = 2.3e-92
Identity = 181/340 (53.24%), Postives = 241/340 (70.88%), Query Frame = 0


Query: 11  AFYVLLILISLYITTSASPTGSVCSSTPDPSYCKSALPNQTGNVYSYGRSSFRKSLSSSQ 70
           +F++  +L S   + + S + ++C +TPDP YCKS  P+  GNV  YG  S RKSLS S+
Sbjct: 12  SFFLQSVLAS---SQTLSNSSTICKTTPDPKYCKSVFPHSQGNVQQYGCFSIRKSLSQSR 71

Query: 71  KFLRLVEKHLRSRSSLTVPAA-RALEDCLLLAGLNIDYLKTSFQTVNATS-----RALTE 130
           KF+R V+++++  + L+ PA  RAL+DC  LAGL +DYL TSF+TVN TS     + L+ 
Sbjct: 72  KFIRTVDRYIKRNAHLSQPAVIRALQDCRFLAGLTMDYLLTSFETVNDTSAKTSFKPLSF 131

Query: 131 MKADDVQSLLSAILTNQQTCLDGIKATA---GSWSLKNGLSQPLASDTKLYSLSLAFFTK 190
            KADD+Q+LLSA LTN+QTCL+G+   A    +W+++ G++ PL +DTKL  +SLA FTK
Sbjct: 132 PKADDIQTLLSAALTNEQTCLEGLTTAASYSATWTVRTGVALPLVNDTKLLGVSLALFTK 191

Query: 191 GWVPKKKKRPTWKVAGRQGG----------FRNGRMSLKMSSRTQAIYEKATRRNLLQTD 250
           GWVPKKKKR  +  A  + G          FRNG + LKM+ +T+A+YE  +RR L   D
Sbjct: 192 GWVPKKKKRAGFAWAQPRSGSSTHTKPFRLFRNGALPLKMTEKTKAVYESLSRRKLADGD 251

Query: 251 ---DGDDDDQIKVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSEGVYEEY 310
              DGDD   + + DIV VSQDG+GNFT I  A+AAAPNN+  + G+FLI+V+ G+YEEY
Sbjct: 252 SNGDGDDGSMVLISDIVTVSQDGTGNFTNITAAVAAAPNNTDGSAGFFLIYVTAGIYEEY 311

Query: 311 VLVAKNKRYLMMIGDGINQTIVTGNRSVVDGWTTFNSATF 329
           + +AKNKRY+MMIGDGINQT+VTGNRSVVDGWTTFNSATF
Sbjct: 312 ISIAKNKRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATF 348

[Close]

BLAST of Chy1G017200 vs. TAIR 10
Match: AT2G47550.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 317.4 bits (812), Expect = 1.4e-86
Identity = 167/308 (54.22%), Postives = 227/308 (73.70%), Query Frame = 0


Query: 26  SASPTGSVCSSTPDPSYCKSALPNQTGNVYSYGRSSFRKSLSSSQKFLRLVEKHLRSRSS 85
           +A+   ++C   PDPSYC+S LPNQ G++YSYGR S R+SLS +++F+ +++  L  +  
Sbjct: 31  NATSPSNICRFAPDPSYCRSVLPNQPGDIYSYGRLSLRRSLSRARRFISMIDAELDRKGK 90

Query: 86  LTVPA-ARALEDCLLLAGLNIDYLKTSFQTVNATSRALTEMKADDVQSLLSAILTNQQTC 145
           +   +   ALEDC  LA L +DYL +S QT ++T + L+  +A+DV + LSA +TN+QTC
Sbjct: 91  VAAKSTVGALEDCKFLASLTMDYLLSSSQTADST-KTLSLSRAEDVHTFLSAAITNEQTC 150

Query: 146 LDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAFFTKGWVPKKKK-RPTWKVAGRQG--- 205
           L+G+K+TA     +NGLS  L +DTKLY +SLA F+KGWVP++++ RP W+   R     
Sbjct: 151 LEGLKSTAS----ENGLSGDLFNDTKLYGVSLALFSKGWVPRRQRSRPIWQPQARFKKFF 210

Query: 206 GFRNGRMSLKMSSRTQAIYEKATRRNLLQTDDGDDDDQIKVRDIVVVSQDGSGNFTTINE 265
           GFRNG++ LKM+ R +A+Y   TRR LLQ+    D D ++V DIV V Q+G+GNFTTIN 
Sbjct: 211 GFRNGKLPLKMTERARAVYNTVTRRKLLQS----DADAVQVSDIVTVIQNGTGNFTTINA 270

Query: 266 AIAAAPNNSAPTDGYFLIFVSEGVYEEYVLVAKNKRYLMMIGDGINQTIVTGNRSVVDGW 325
           AIAAAPN +  ++GYFLI+V+ G+YEEYV V KNKRY+MMIGDGINQT++TGNRSVVDGW
Sbjct: 271 AIAAAPNKTDGSNGYFLIYVTAGLYEEYVEVPKNKRYVMMIGDGINQTVITGNRSVVDGW 329

Query: 326 TTFNSATF 329
           TTFNSATF
Sbjct: 331 TTFNSATF 329

[Close]

BLAST of Chy1G017200 vs. TAIR 10
Match: AT4G02330.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 315.5 bits (807), Expect = 5.4e-86
Identity = 176/334 (52.69%), Postives = 239/334 (71.56%), Query Frame = 0


Query: 14  VLLILISLYITTS----ASPTGSVCSSTPDPSYCKSALPNQT-GNVYSYGRSSFRKSLSS 73
           + L L +L+I +     ++ + ++C +TPDP +CKS  P  + G+V  YGR S RKSL+ 
Sbjct: 11  LFLSLQTLFIASQTLLPSNSSSTICKTTPDPKFCKSVFPQTSQGDVREYGRFSLRKSLTQ 70

Query: 74  SQKFLRLVEKHL-RSRSSLTVPAARALEDCLLLAGLNIDYLKTSFQTVNAT--SRALTEM 133
           S+KF R ++++L R+ + L+  A  AL+DC  LA L  DYL TSF+TVN T  S+ L+  
Sbjct: 71  SRKFTRTIDRYLKRNNALLSQSAVGALQDCRYLASLTTDYLITSFETVNITTSSKTLSFS 130

Query: 134 KADDVQSLLSAILTNQQTCLDGIK-ATAGSWSLKNGLSQPLASDTKLYSLSLAFFTKGWV 193
           KAD++Q+LLSA LTN+QTCLDGI  A + SW+++NG++ PL +DTKL+S+SLA FTKGWV
Sbjct: 131 KADEIQTLLSAALTNEQTCLDGINTAASSSWTIRNGVALPLINDTKLFSVSLALFTKGWV 190

Query: 194 PKKKKRP---TWKVAGRQGG-------FRNGRMSLKMSSRTQAIYEKATRRNLLQTDDGD 253
           PKKKK+    +W               FRNG + LKM+  T+A+YE  +RR L   DD +
Sbjct: 191 PKKKKQVASYSWAHPKNTHSHTKPFRHFRNGALPLKMTEHTRAVYESLSRRKL--ADDDN 250

Query: 254 DDDQIKVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSEGVYEEYVLVAKN 313
           D + + V DIV V+Q+G+GNFTTI EA+ +APN +  T GYF+I+V+ GVYEE V++AKN
Sbjct: 251 DVNTVLVSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIYVTSGVYEENVVIAKN 310

Query: 314 KRYLMMIGDGINQTIVTGNRSVVDGWTTFNSATF 329
           KRYLMMIGDGIN+T+VTGNR+VVDGWTTFNSATF
Sbjct: 311 KRYLMMIGDGINRTVVTGNRNVVDGWTTFNSATF 342

[Close]

BLAST of Chy1G017200 vs. TAIR 10
Match: AT3G10720.2 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 171.8 bits (434), Expect = 9.7e-43
Identity = 117/322 (36.34%), Postives = 178/322 (55.28%), Query Frame = 0


Query: 20  SLYITTSASPTGSVCSSTPDPSYCK---SALPNQTGNVYSYGRSSFRKSLSSSQKFLRLV 79
           SL  + S SP+   C STP P  C+   +A+ +   + Y YG+ + ++ L  + +  +++
Sbjct: 70  SLPPSQSQSPS-LACKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQASRLSKVI 129

Query: 80  EKHLR----SRSSLTVPAARALEDCLLLAGLNIDYLKTSFQTVNATSRALTEMKADDVQS 139
             + R       S T     A+ DC  L+ L+++YL+T   T   T++ +T    + V S
Sbjct: 130 TSYARRVESKPGSATAEEIGAVADCGELSELSVNYLET-VTTELKTAQVMTAALVEHVNS 189

Query: 140 LLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAFFTKGWVPKKKKRPT 199
           LLS ++TNQQTCLDG+      ++    +  P+ + T+LYS+SL   +       K+   
Sbjct: 190 LLSGVVTNQQTCLDGLVEAKSGFAA--AIGSPMGNLTRLYSISLGLVSHALNRNLKRFKA 249

Query: 200 WK---VAGRQGGFRNGRMSLKMSSRTQAIYEK---ATRRNLLQTDDGDDDDQIKVRDIVV 259
            K   + G    +R    +L    R     +K    T RNL +  +      I V   V+
Sbjct: 250 SKGKILGGGNSTYREPLETLIKGLRKTCDNDKDCRKTSRNLGELGE-TSGGSILVSKAVI 309

Query: 260 VSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSEGVYEEYVLVAKNKRYLMMIGDGIN 319
           V    S NFTTI +AIAAAPNN+ P DGYF+I+  EGVYEEY++V  NK+ LM++GDGIN
Sbjct: 310 VGPFKSDNFTTITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMGDGIN 369

Query: 320 QTIVTGNRSVVDGWTTFNSATF 329
           +TI+TGN +VVDGWTT+N ++F
Sbjct: 370 KTIITGNHNVVDGWTTYNCSSF 386

[Close]

BLAST of Chy1G017200 vs. TAIR 10
Match: AT5G04970.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 164.9 bits (416), Expect = 1.2e-40
Identity = 111/321 (34.58%), Postives = 172/321 (53.58%), Query Frame = 0


Query: 26  SASPTGSVCSSTPDPSYCK---SALPNQTGNVYSYGRSSFRKSLSSSQKFLRLVEKHLR- 85
           S  P    C STP P  C+   SA+ +   + Y YG+ + ++ L  +++  +++ +  + 
Sbjct: 80  SPLPPNIACKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQARRLSKVINRFAQR 139

Query: 86  ---SRSSLTVPAARALEDCLLLAGLNIDYLKTSFQTVNATSRALTEMKADDVQSLLSAIL 145
                 + TV    A+ DC  LA L+++YL+T  + + A +  +T    D V SLL  ++
Sbjct: 140 VEADPGTSTVEEVSAVADCGELAELSVEYLETVTEELKA-AELMTAALVDRVTSLLGGVV 199

Query: 146 TNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAFFTKGWVPKKKKRPTWKVAGR 205
           TNQQTCLDG+      ++    +  PL + T+LYS+SL     G V     R   +  G 
Sbjct: 200 TNQQTCLDGLVDAKSGFA--TAIGTPLGNLTRLYSVSL-----GLVSHALNRNLKRYKGS 259

Query: 206 QGG-FRNGRMSLKMSSRTQAIYEKAT----------RRNLLQTDDGDDDDQIKVRDIVVV 265
           +G  F  G   ++    T     + T           RNL +  +      I VR+ V V
Sbjct: 260 KGKIFGGGNKPVREPLETLIKVLRKTCDKGKDCRKANRNLGELGE-TSGGSILVREAVTV 319

Query: 266 SQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSEGVYEEYVLVAKNKRYLMMIGDGINQ 325
               + NF TI EA+AAAPN++ P  GYF+I+   G+YEEYV+++  KR +M+IGDGIN+
Sbjct: 320 GPYETDNFPTITEAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKKRNIMLIGDGINK 379

Query: 326 TIVTGNRSVVDGWTTFNSATF 329
           TI++GN S +DGWTT+NS+TF
Sbjct: 380 TIISGNHSFIDGWTTYNSSTF 391

The following BLAST results are available for this feature:

BLAST of Chy1G017200 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SRX4	3.2e-91	53.24	Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=37...	[more]
O22256	2.0e-85	54.22	Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana OX=3...	[more]
Q8RXK7	7.6e-85	52.69	Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3...	[more]
Q94CB1	1.4e-41	36.34	Probable pectinesterase/pectinesterase inhibitor 25 OS=Arabidopsis thaliana OX=3...	[more]
Q9FF77	1.7e-39	34.58	Probable pectinesterase/pectinesterase inhibitor 47 OS=Arabidopsis thaliana OX=3...	[more]

BLAST of Chy1G017200 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KB12	1.1e-171	97.87	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_7G405820 PE=3 SV=1	[more]
A0A5D3D3H4	1.6e-162	94.53	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G00132...	[more]
A0A1S3BSI2	1.6e-162	94.53	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103492791 PE=3 SV=1	[more]
A0A6J1GQE6	6.6e-148	86.10	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111456155 PE=3 SV=1	[more]
A0A6J1JVJ5	1.8e-145	84.38	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111487941 PE=3 SV=1	[more]

BLAST of Chy1G017200 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004136013.1	2.60e-217	97.87	probable pectinesterase/pectinesterase inhibitor 7 [Cucumis sativus] >KGN44976.1...	[more]
XP_008451544.1	1.73e-205	94.53	PREDICTED: probable pectinesterase/pectinesterase inhibitor 7 [Cucumis melo] >KA...	[more]
XP_038896831.1	3.33e-194	88.79	probable pectinesterase/pectinesterase inhibitor 7 [Benincasa hispida]	[more]
KAG7014346.1	6.66e-187	87.42	putative pectinesterase/pectinesterase inhibitor 41, partial [Cucurbita argyrosp...	[more]
XP_022953705.1	1.90e-186	87.12	probable pectinesterase/pectinesterase inhibitor 7 [Cucurbita moschata]	[more]

BLAST of Chy1G017200 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G02810.1	2.3e-92	53.24	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT2G47550.1	1.4e-86	54.22	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT4G02330.1	5.4e-86	52.69	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT3G10720.2	9.7e-43	36.34	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT5G04970.1	1.2e-40	34.58	Plant invertase/pectin methylesterase inhibitor superfamily	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 24..178 e-value: 4.8E-22 score: 89.2
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 32..177 e-value: 2.3E-17 score: 63.7
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 238..333 e-value: 5.8E-25 score: 90.3
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 245..329 e-value: 5.0E-25 score: 87.8
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 30..181 e-value: 1.9E-21 score: 78.7
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 32..181
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 25..329
None	No IPR available	PANTHER	PTHR31707:SF332	PECTINESTERASE	coord: 25..329
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 32..183 e-value: 1.70713E-29 score: 109.065
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 243..328

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy1G017200.1	Chy1G017200.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0045490	pectin catabolic process
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0030599	pectinesterase activity
molecular_function	GO:0046910	pectinesterase inhibitor activity
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity
molecular_function	GO:0004857	enzyme inhibitor activity