Chy1G016910 (gene) Cucumber (hystrix) v1

Overview


Name	Chy1G016910
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	Plant invertase/pectin methylesterase inhibitor
Location	chrH01: 22795480 .. 22796488 (+)
RNA-Seq Expression	Chy1G016910
Synteny	Chy1G016910

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTACAGAAATTTCATGATCTACAAGTCAAAGACGTATAGTAAATTCTGGTAAAACAGATACCAAATTTTCCAAGATTTCCTTTATTGTAAAGTTCATGAGAACTCACCTGCCATTCACATGTTCTTCACCTTCTTAACTTATTTCACTTCATCACTACTTCCCCTCCCCTTCTTCAATGCATTCACCATCTCCACGCCTAATTTATAGCCAATACCTGCAAAAAGTTATCCAAAATGACTTTTCAAGTCACCCCCATACCCTTTTCCCCACTATATAATCCACCCATAATCTCATTAATCCCCACATTAGTCTCAATCTCATTCACAAATCACCCCACTATATACTGATTTCATAGAAATTTATATTCTATCCCCACATAACTTTTTCACTTCTCTCTGACATTTCATCAAACATGTTGCGTGCACCCAGTAGAAGAGCATCAATCGCAGCCCTTTTGGCCCTCATCTCCATTCTCCCATGGCTGACCCACTCCGCCAAAACGAGCTATGTCCAAGAAGCCTGCAGGGTAACACGACACCAAGATCTCTGCATTCAATCCCTGTCGCCATTTTCAAGCGCCGCCAAGCGCAGCCCAACAAAGTGGGCTCGAGCGGGGGTGTCGGTGACGATAACAGAGGCAAAAAAAGTAGCAGGACTCTTGGAGAGGTTGAAGAACAACAAGCGGATGAAGGGGAGAAACAGGGCAGCAGTTTTGGACTGTGTGGAGGTGTTTGAAGCCGCCATTGATGAGCTTCACAAGTCGCTTGGGGTTCTTAGAAGATTGAGTAGGAGGAATTTTGACGCACAGATGGGGGATCTAACAACGTGGGTCAGCGCTGCACTAACAGATGAAGATACATGCTTAGAAGGATTTGAAGGTGAAAGGGGAAAAGCAGTGACTTTGCTAAGGAATCGAGTGGTGAAAGTTGGTTATATTACGAGTAATGCACTTGCTTTGGTTAACAAATTGGCTGCCTCTAGTTTCGAAACTACAATAAATATGTAA

mRNA sequence

ATGTACAGAAATTTCATGATCTACAAGTCAAAGACGTATAGTAAATTCTGTAGAAGAGCATCAATCGCAGCCCTTTTGGCCCTCATCTCCATTCTCCCATGGCTGACCCACTCCGCCAAAACGAGCTATGTCCAAGAAGCCTGCAGGGTAACACGACACCAAGATCTCTGCATTCAATCCCTGTCGCCATTTTCAAGCGCCGCCAAGCGCAGCCCAACAAAGTGGGCTCGAGCGGGGGTGTCGGTGACGATAACAGAGGCAAAAAAAGTAGCAGGACTCTTGGAGAGGTTGAAGAACAACAAGCGGATGAAGGGGAGAAACAGGGCAGCAGTTTTGGACTGTGTGGAGGTGTTTGAAGCCGCCATTGATGAGCTTCACAAGTCGCTTGGGGTTCTTAGAAGATTGAGTAGGAGGAATTTTGACGCACAGATGGGGGATCTAACAACGTGGGTCAGCGCTGCACTAACAGATGAAGATACATGCTTAGAAGGATTTGAAGGTGAAAGGGGAAAAGCAGTGACTTTGCTAAGGAATCGAGTGGTGAAAGTTGGTTATATTACGAGTAATGCACTTGCTTTGGTTAACAAATTGGCTGCCTCTAGTTTCGAAACTACAATAAATATGTAA

Coding sequence (CDS)

ATGTACAGAAATTTCATGATCTACAAGTCAAAGACGTATAGTAAATTCTGTAGAAGAGCATCAATCGCAGCCCTTTTGGCCCTCATCTCCATTCTCCCATGGCTGACCCACTCCGCCAAAACGAGCTATGTCCAAGAAGCCTGCAGGGTAACACGACACCAAGATCTCTGCATTCAATCCCTGTCGCCATTTTCAAGCGCCGCCAAGCGCAGCCCAACAAAGTGGGCTCGAGCGGGGGTGTCGGTGACGATAACAGAGGCAAAAAAAGTAGCAGGACTCTTGGAGAGGTTGAAGAACAACAAGCGGATGAAGGGGAGAAACAGGGCAGCAGTTTTGGACTGTGTGGAGGTGTTTGAAGCCGCCATTGATGAGCTTCACAAGTCGCTTGGGGTTCTTAGAAGATTGAGTAGGAGGAATTTTGACGCACAGATGGGGGATCTAACAACGTGGGTCAGCGCTGCACTAACAGATGAAGATACATGCTTAGAAGGATTTGAAGGTGAAAGGGGAAAAGCAGTGACTTTGCTAAGGAATCGAGTGGTGAAAGTTGGTTATATTACGAGTAATGCACTTGCTTTGGTTAACAAATTGGCTGCCTCTAGTTTCGAAACTACAATAAATATGTAA

Protein sequence

MYRNFMIYKSKTYSKFCRRASIAALLALISILPWLTHSAKTSYVQEACRVTRHQDLCIQSLSPFSSAAKRSPTKWARAGVSVTITEAKKVAGLLERLKNNKRMKGRNRAAVLDCVEVFEAAIDELHKSLGVLRRLSRRNFDAQMGDLTTWVSAALTDEDTCLEGFEGERGKAVTLLRNRVVKVGYITSNALALVNKLAASSFETTINM*

Homology

[Close]

BLAST of Chy1G016910 vs. ExPASy Swiss-Prot
Match: O22244 (Pectinesterase inhibitor 6 OS=Arabidopsis thaliana OX=3702 GN=PMEI6 PE=2 SV=1)

HSP 1 Score: 142.5 bits (358), Expect = 5.4e-33
Identity = 77/161 (47.83%), Postives = 109/161 (67.70%), Query Frame = 0


Query: 41  TSYVQEACRVTRHQDLCIQSLSPFSSAAKRSPTKWARAGVSVTITEAKKVAGLLERLKNN 100
           ++YV+ AC VTR+  LC+++L PF+  A+ + +KWARA V+VTIT+ K+V  LL  LK  
Sbjct: 46  STYVRNACNVTRYNRLCVRTLWPFAIVARNNTSKWARASVAVTITDTKRVLRLL--LKTQ 105

Query: 101 KRMKGRN-RAAVLDCVEVFEAAIDELHKSLGVLRRLSRRNFDAQMGDLTTWVSAALTDED 160
           +   G + R A+ DC E+F  ++D L+KSL VLR L+   F  Q+ DL TW+SAALTD+D
Sbjct: 106 RSAVGESERIALSDCRELFVDSLDNLYKSLAVLRTLNADEFQRQISDLATWLSAALTDDD 165

Query: 161 TCLEGFE--GERGKAVTLLRNRVVKVGYITSNALALVNKLA 199
           TCL+GFE    R + V ++R +  K   + SNALAL+ KLA
Sbjct: 166 TCLDGFEETSSRTRTVRMVRRKATKCMRLCSNALALLKKLA 204

[Close]

BLAST of Chy1G016910 vs. ExPASy Swiss-Prot
Match: P17407 (21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1)

HSP 1 Score: 114.0 bits (284), Expect = 2.0e-24
Identity = 64/178 (35.96%), Postives = 105/178 (58.99%), Query Frame = 0


Query: 27  ALISILPWLTHSAKTSYVQEACRVTRHQDLCIQSLSPFSSAAKRSPTKWARAGVSVTITE 86
           AL+ IL   + +    +++ +C +T +  +C QSLS ++   + +P + A   + V++T 
Sbjct: 12  ALLVILAAASAAPANQFIKTSCTLTTYPAVCEQSLSAYAKTIQNNPQELASTALQVSLTR 71

Query: 87  AKKVAGLLERLKNNKRMKGRNRAAVLDCVEVFEAAIDELHKSLGVLRRLSR---RNFDAQ 146
            ++    ++RL   K +K R  AA+ DC+E  E ++D + +S   ++ LS     +F  +
Sbjct: 72  TQQAQTFMKRLNKFKGLKARQYAAIHDCLEEVEDSLDRVSRSCDEMKNLSHAKGNDFTFR 131

Query: 147 MGDLTTWVSAALTDEDTCLEGFEGE--RGKAVTLLRNRVVKVGYITSNALALVNKLAA 200
           M ++ TWVSAALTDE TC++GF G+   GK    +R +VV V  +TSNALALVN  AA
Sbjct: 132 MSNVETWVSAALTDETTCMDGFAGKGMDGKIKESVRAQVVAVARVTSNALALVNNFAA 189

[Close]

BLAST of Chy1G016910 vs. ExPASy Swiss-Prot
Match: Q84WE4 (Pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PMEI3 PE=2 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 3.0e-23
Identity = 59/167 (35.33%), Postives = 101/167 (60.48%), Query Frame = 0


Query: 37  HSAKTSYVQEACRVTRHQDLCIQSLSPFSSAAKRSPTKWARAGVSVTITEAKKVAGLLER 96
           H+     V  +C    +  LC+++LS +S     +    A+A + ++++ A+  A  L  
Sbjct: 27  HNGAEDIVHSSCEHASYPSLCVRTLSSYSGPTITNRRDLAQAAIKISLSHAQSAAKKLAV 86

Query: 97  LKNNKRMKGRNRAAVLDCVEVFEAAIDELHKSLGVLRRL-----SRRNFDAQMGDLTTWV 156
           ++++   K + +AA++DCVE+   ++DEL ++LGVL+ L     S + F  QM +  TW 
Sbjct: 87  VRDSVGKKKQEKAALVDCVEMIGDSVDELSRTLGVLKHLRVSGGSAKEFRWQMSNAQTWA 146

Query: 157 SAALTDEDTCLEGFEG-ERGKAVTLLRNRVVKVGYITSNALALVNKL 198
           SAALTD+DTCL+GF+G + G+  T ++  + KV  +TSNAL +VN+L
Sbjct: 147 SAALTDDDTCLDGFQGMDDGEIKTEVKQWMTKVARVTSNALYMVNQL 193

[Close]

BLAST of Chy1G016910 vs. ExPASy Swiss-Prot
Match: Q9SB37 (Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 2.5e-22
Identity = 61/168 (36.31%), Postives = 92/168 (54.76%), Query Frame = 0


Query: 38  SAKTSYVQEACRVTRHQDLCIQSLSPFSSAAKRSPTKWARAGVSVTITEAKKVAGLLERL 97
           S  T +++ +C  T + D C QSLS ++S  K+ P K A   ++V+I  AK     +  +
Sbjct: 32  SGDTEFIKASCETTSYPDRCFQSLSSYASEIKKQPRKLAETALAVSIARAKSAKTYVSEM 91

Query: 98  KNNKRMKGRNRAAVLDCVEVFEAAIDELHKSLGVLRRL----SRRNFDAQMGDLTTWVSA 157
            + K +  R   AV DC+E     +D L  SL  L+ L    S  +F   + ++ TW SA
Sbjct: 92  TDYKGITKRQHEAVADCLEEMGDTVDRLSNSLKELKHLEEGDSGEDFWFCLSNVRTWTSA 151

Query: 158 ALTDEDTCLEGFEGE--RGKAVTLLRNRVVKVGYITSNALALVNKLAA 200
           ALTDE  C++GF G+   G+  +L+R  +V V   TSNALAL+N  A+
Sbjct: 152 ALTDETACMDGFGGKAMAGELKSLIRTHIVSVAEETSNALALINDFAS 199

[Close]

BLAST of Chy1G016910 vs. ExPASy Swiss-Prot
Match: Q9SI74 (Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 3.3e-22
Identity = 57/170 (33.53%), Postives = 102/170 (60.00%), Query Frame = 0


Query: 36  THSAKTS--YVQEACRVTRHQDLCIQSLSPFSSAAKRSPTKWARAGVSVTITEAKKVAGL 95
           T+S +T+  Y++ +C +T ++ +C  SLSP++S  + +P K A   +++T++ AK  +  
Sbjct: 138 TYSNQTNLDYIKTSCNITLYKTICYNSLSPYASTIRSNPQKLAVIALNLTLSSAKSASKF 197

Query: 96  LERLKNNKRMKGRNRAAVLDCVEVFEAAIDELHKSLGVLRRLSRRN---FDAQMGDLTTW 155
           ++ + +   +      AV DCVE    ++  L  S+  L  ++ ++   F+  M D+ TW
Sbjct: 198 VKNISHGGGLTRLEVVAVADCVEEIGDSVTSLQDSIRELDSINYKDSAKFEMVMSDVETW 257

Query: 156 VSAALTDEDTCLEGFEGERGKAVTLLRNRVVKVGYITSNALALVNKLAAS 201
           VSAALT++DTC++GF   +     L+R  VV+V  +TSNALAL+N  A++
Sbjct: 258 VSAALTNDDTCMDGFSLVKTAVKDLVRRHVVEVARLTSNALALINMYAST 307

[Close]

BLAST of Chy1G016910 vs. ExPASy TrEMBL
Match: A0A0A0KAX0 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G397050 PE=4 SV=1)

HSP 1 Score: 357.5 bits (916), Expect = 3.9e-95
Identity = 185/191 (96.86%), Postives = 187/191 (97.91%), Query Frame = 0


Query: 18  RRASIAALLALISILPWLTHSAKTSYVQEACRVTRHQDLCIQSLSPFSSAAKRSPTKWAR 77
           RRASI ALLALISILPWLTHSAKTSYVQEACRVTRHQDLCIQSLSPFSSAAKRSPTKWAR
Sbjct: 7   RRASIIALLALISILPWLTHSAKTSYVQEACRVTRHQDLCIQSLSPFSSAAKRSPTKWAR 66

Query: 78  AGVSVTITEAKKVAGLLERLKNNKRMKGRNRAAVLDCVEVFEAAIDELHKSLGVLRRLSR 137
           AGVSVTITEAKKVAGLL RLKNNKRMKGRNRAAVLDCVEVFEAAIDELH+SLGVLRRLSR
Sbjct: 67  AGVSVTITEAKKVAGLLGRLKNNKRMKGRNRAAVLDCVEVFEAAIDELHRSLGVLRRLSR 126

Query: 138 RNFDAQMGDLTTWVSAALTDEDTCLEGFEGERGKAVTLLRNRVVKVGYITSNALALVNKL 197
           RNFDAQMGDLTTWVSAALTDEDTC+EGFEGE GK VTLLRNRVVKVGYITSNALALVNKL
Sbjct: 127 RNFDAQMGDLTTWVSAALTDEDTCVEGFEGEEGKVVTLLRNRVVKVGYITSNALALVNKL 186

Query: 198 AASSFETTINM 209
           AASSFETTINM
Sbjct: 187 AASSFETTINM 197

[Close]

BLAST of Chy1G016910 vs. ExPASy TrEMBL
Match: A0A5D3D1R1 (21 kDa protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G00630 PE=4 SV=1)

HSP 1 Score: 343.2 bits (879), Expect = 7.7e-91
Identity = 176/190 (92.63%), Postives = 182/190 (95.79%), Query Frame = 0


Query: 19  RASIAALLALISILPWLTHSAKTSYVQEACRVTRHQDLCIQSLSPFSSAAKRSPTKWARA 78
           RASIAALLALISILPWLTHSAKTSYVQEACRVT HQDLCIQSLSPFSS AKRSPTKWARA
Sbjct: 8   RASIAALLALISILPWLTHSAKTSYVQEACRVTLHQDLCIQSLSPFSSTAKRSPTKWARA 67

Query: 79  GVSVTITEAKKVAGLLERLKNNKRMKGRNRAAVLDCVEVFEAAIDELHKSLGVLRRLSRR 138
           GVSVTITEAKKVA LL RLKNNKRMKGRNRAAVLDCVEVF+AAIDELH+SLGVLRRLSRR
Sbjct: 68  GVSVTITEAKKVAALLGRLKNNKRMKGRNRAAVLDCVEVFDAAIDELHRSLGVLRRLSRR 127

Query: 139 NFDAQMGDLTTWVSAALTDEDTCLEGFEGERGKAVTLLRNRVVKVGYITSNALALVNKLA 198
           NFD+QMGDLTTWVSAALTDEDTC+EGFEGERGK V+LLRNRVVKVGYITSNALALVNKLA
Sbjct: 128 NFDSQMGDLTTWVSAALTDEDTCVEGFEGERGKVVSLLRNRVVKVGYITSNALALVNKLA 187

Query: 199 ASSFETTINM 209
            S FET +NM
Sbjct: 188 TSGFETALNM 197

[Close]

BLAST of Chy1G016910 vs. ExPASy TrEMBL
Match: A0A1S3BSW5 (21 kDa protein OS=Cucumis melo OX=3656 GN=LOC103492810 PE=4 SV=1)

HSP 1 Score: 343.2 bits (879), Expect = 7.7e-91
Identity = 176/190 (92.63%), Postives = 182/190 (95.79%), Query Frame = 0


Query: 19  RASIAALLALISILPWLTHSAKTSYVQEACRVTRHQDLCIQSLSPFSSAAKRSPTKWARA 78
           RASIAALLALISILPWLTHSAKTSYVQEACRVT HQDLCIQSLSPFSS AKRSPTKWARA
Sbjct: 8   RASIAALLALISILPWLTHSAKTSYVQEACRVTLHQDLCIQSLSPFSSTAKRSPTKWARA 67

Query: 79  GVSVTITEAKKVAGLLERLKNNKRMKGRNRAAVLDCVEVFEAAIDELHKSLGVLRRLSRR 138
           GVSVTITEAKKVA LL RLKNNKRMKGRNRAAVLDCVEVF+AAIDELH+SLGVLRRLSRR
Sbjct: 68  GVSVTITEAKKVAALLGRLKNNKRMKGRNRAAVLDCVEVFDAAIDELHRSLGVLRRLSRR 127

Query: 139 NFDAQMGDLTTWVSAALTDEDTCLEGFEGERGKAVTLLRNRVVKVGYITSNALALVNKLA 198
           NFD+QMGDLTTWVSAALTDEDTC+EGFEGERGK V+LLRNRVVKVGYITSNALALVNKLA
Sbjct: 128 NFDSQMGDLTTWVSAALTDEDTCVEGFEGERGKVVSLLRNRVVKVGYITSNALALVNKLA 187

Query: 199 ASSFETTINM 209
            S FET +NM
Sbjct: 188 TSGFETALNM 197

[Close]

BLAST of Chy1G016910 vs. ExPASy TrEMBL
Match: A0A6J1L076 (pectinesterase inhibitor 6 OS=Cucurbita maxima OX=3661 GN=LOC111497889 PE=4 SV=1)

HSP 1 Score: 303.1 bits (775), Expect = 8.8e-79
Identity = 156/190 (82.11%), Postives = 166/190 (87.37%), Query Frame = 0


Query: 19  RASIAALLALISILPWLTHSAKTSYVQEACRVTRHQDLCIQSLSPFSSAAKRSPTKWARA 78
           R SI  LLA I+ILPWLTHSA+TSYVQEAC VT H DLCI+SLSPFSS AKRSPTKWARA
Sbjct: 8   RTSIVTLLAFIAILPWLTHSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKRSPTKWARA 67

Query: 79  GVSVTITEAKKVAGLLERLKNNKRMKGRNRAAVLDCVEVFEAAIDELHKSLGVLRRLSRR 138
            VSVTITEAK VA  L RLKNN+RMKGRNRAAV DCVEVFE AIDELH+SLGVLRRLSR 
Sbjct: 68  SVSVTITEAKAVARFLVRLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGVLRRLSRS 127

Query: 139 NFDAQMGDLTTWVSAALTDEDTCLEGFEGERGKAVTLLRNRVVKVGYITSNALALVNKLA 198
            FDAQMGDLTTWVSAALTDEDTC++GFEGERG+ V L+RNRVVK  YITSNALALVNKLA
Sbjct: 128 KFDAQMGDLTTWVSAALTDEDTCVDGFEGERGRLVNLIRNRVVKATYITSNALALVNKLA 187

Query: 199 ASSFETTINM 209
           AS FET  N+
Sbjct: 188 ASGFETAPNL 197

[Close]

BLAST of Chy1G016910 vs. ExPASy TrEMBL
Match: A0A6J1H9V5 (pectinesterase inhibitor 6-like OS=Cucurbita moschata OX=3662 GN=LOC111460929 PE=4 SV=1)

HSP 1 Score: 302.0 bits (772), Expect = 2.0e-78
Identity = 156/190 (82.11%), Postives = 165/190 (86.84%), Query Frame = 0


Query: 19  RASIAALLALISILPWLTHSAKTSYVQEACRVTRHQDLCIQSLSPFSSAAKRSPTKWARA 78
           R SI  LLA ISILPWLTHSA+TSYVQEAC VT H DLCI+SLSPFSS AKRSPTKWARA
Sbjct: 70  RTSIVTLLAFISILPWLTHSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKRSPTKWARA 129

Query: 79  GVSVTITEAKKVAGLLERLKNNKRMKGRNRAAVLDCVEVFEAAIDELHKSLGVLRRLSRR 138
            VSVTITEAK VA  L RLKNN+RMKGRNRAAV DCVEVFE AIDELH+SLGVLRRLSR 
Sbjct: 130 SVSVTITEAKAVARFLVRLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGVLRRLSRS 189

Query: 139 NFDAQMGDLTTWVSAALTDEDTCLEGFEGERGKAVTLLRNRVVKVGYITSNALALVNKLA 198
            FDAQMGDLTTWVSAALTDEDTC++GFEGERG+ V L+RNR VK  YITSNALALVNKLA
Sbjct: 190 KFDAQMGDLTTWVSAALTDEDTCVDGFEGERGRLVNLIRNRAVKATYITSNALALVNKLA 249

Query: 199 ASSFETTINM 209
           AS FET  N+
Sbjct: 250 ASGFETGPNL 259

[Close]

BLAST of Chy1G016910 vs. NCBI nr
Match: XP_011659361.1 (pectinesterase inhibitor 6 [Cucumis sativus])

HSP 1 Score: 358 bits (919), Expect = 6.94e-124
Identity = 185/191 (96.86%), Postives = 187/191 (97.91%), Query Frame = 0


Query: 18  RRASIAALLALISILPWLTHSAKTSYVQEACRVTRHQDLCIQSLSPFSSAAKRSPTKWAR 77
           RRASI ALLALISILPWLTHSAKTSYVQEACRVTRHQDLCIQSLSPFSSAAKRSPTKWAR
Sbjct: 7   RRASIIALLALISILPWLTHSAKTSYVQEACRVTRHQDLCIQSLSPFSSAAKRSPTKWAR 66

Query: 78  AGVSVTITEAKKVAGLLERLKNNKRMKGRNRAAVLDCVEVFEAAIDELHKSLGVLRRLSR 137
           AGVSVTITEAKKVAGLL RLKNNKRMKGRNRAAVLDCVEVFEAAIDELH+SLGVLRRLSR
Sbjct: 67  AGVSVTITEAKKVAGLLGRLKNNKRMKGRNRAAVLDCVEVFEAAIDELHRSLGVLRRLSR 126

Query: 138 RNFDAQMGDLTTWVSAALTDEDTCLEGFEGERGKAVTLLRNRVVKVGYITSNALALVNKL 197
           RNFDAQMGDLTTWVSAALTDEDTC+EGFEGE GK VTLLRNRVVKVGYITSNALALVNKL
Sbjct: 127 RNFDAQMGDLTTWVSAALTDEDTCVEGFEGEEGKVVTLLRNRVVKVGYITSNALALVNKL 186

Query: 198 AASSFETTINM 208
           AASSFETTINM
Sbjct: 187 AASSFETTINM 197

[Close]

BLAST of Chy1G016910 vs. NCBI nr
Match: XP_008451567.1 (PREDICTED: 21 kDa protein [Cucumis melo] >KAA0067994.1 21 kDa protein [Cucumis melo var. makuwa] >TYK18133.1 21 kDa protein [Cucumis melo var. makuwa])

HSP 1 Score: 344 bits (882), Expect = 3.02e-118
Identity = 176/190 (92.63%), Postives = 182/190 (95.79%), Query Frame = 0


Query: 19  RASIAALLALISILPWLTHSAKTSYVQEACRVTRHQDLCIQSLSPFSSAAKRSPTKWARA 78
           RASIAALLALISILPWLTHSAKTSYVQEACRVT HQDLCIQSLSPFSS AKRSPTKWARA
Sbjct: 8   RASIAALLALISILPWLTHSAKTSYVQEACRVTLHQDLCIQSLSPFSSTAKRSPTKWARA 67

Query: 79  GVSVTITEAKKVAGLLERLKNNKRMKGRNRAAVLDCVEVFEAAIDELHKSLGVLRRLSRR 138
           GVSVTITEAKKVA LL RLKNNKRMKGRNRAAVLDCVEVF+AAIDELH+SLGVLRRLSRR
Sbjct: 68  GVSVTITEAKKVAALLGRLKNNKRMKGRNRAAVLDCVEVFDAAIDELHRSLGVLRRLSRR 127

Query: 139 NFDAQMGDLTTWVSAALTDEDTCLEGFEGERGKAVTLLRNRVVKVGYITSNALALVNKLA 198
           NFD+QMGDLTTWVSAALTDEDTC+EGFEGERGK V+LLRNRVVKVGYITSNALALVNKLA
Sbjct: 128 NFDSQMGDLTTWVSAALTDEDTCVEGFEGERGKVVSLLRNRVVKVGYITSNALALVNKLA 187

Query: 199 ASSFETTINM 208
            S FET +NM
Sbjct: 188 TSGFETALNM 197

[Close]

BLAST of Chy1G016910 vs. NCBI nr
Match: XP_038896715.1 (pectinesterase inhibitor 6-like [Benincasa hispida])

HSP 1 Score: 318 bits (816), Expect = 3.43e-108
Identity = 165/189 (87.30%), Postives = 172/189 (91.01%), Query Frame = 0


Query: 19  RASIAALLALISILPWLTHSAKTSYVQEACRVTRHQDLCIQSLSPFSSAAKRSPTKWARA 78
           RASIA  LALISILP LTHS KTSYV+EAC VTRHQDLCIQSL+PFSS AKRSP KWARA
Sbjct: 8   RASIATFLALISILPRLTHSTKTSYVREACSVTRHQDLCIQSLTPFSSTAKRSPAKWARA 67

Query: 79  GVSVTITEAKKVAGLLERLKNNKRMKGRNRAAVLDCVEVFEAAIDELHKSLGVLRRLSRR 138
           GVSVTITEAK VAGLL RLKNNKRMKGRNRAAV DCVEVFEAAIDELH+SLGVLRRLSR 
Sbjct: 68  GVSVTITEAKTVAGLLGRLKNNKRMKGRNRAAVSDCVEVFEAAIDELHRSLGVLRRLSRS 127

Query: 139 NFDAQMGDLTTWVSAALTDEDTCLEGFEGERGKAVTLLRNRVVKVGYITSNALALVNKLA 198
           NFDAQ+GDLTTWVSAALTDEDTC+EGFEGERGK V L+RNRV+KVGYITSNALALVNKLA
Sbjct: 128 NFDAQIGDLTTWVSAALTDEDTCVEGFEGERGKLVNLIRNRVLKVGYITSNALALVNKLA 187

Query: 199 ASSFETTIN 207
           AS  ET  N
Sbjct: 188 ASGLETAPN 196

[Close]

BLAST of Chy1G016910 vs. NCBI nr
Match: KAE8646342.1 (hypothetical protein Csa_016554 [Cucumis sativus])

HSP 1 Score: 311 bits (798), Expect = 1.10e-104
Identity = 160/171 (93.57%), Postives = 163/171 (95.32%), Query Frame = 0


Query: 18  RRASIAALLALISILPWLTHSAKTSYVQEACRVTRHQDLCIQSLSPFSSAAKRSPTKWAR 77
           RRASI ALLALISILPWLTHSAKTSYVQEACRVTRHQDLCIQSLSPFSSAAKRSPTKWAR
Sbjct: 7   RRASIIALLALISILPWLTHSAKTSYVQEACRVTRHQDLCIQSLSPFSSAAKRSPTKWAR 66

Query: 78  AGVSVTITEAKKVAGLLERLKNNKRMKGRNRAAVLDCVEVFEAAIDELHKSLGVLRRLSR 137
           AGVSVTITEAKKVAGLL RLKNNKRMKGRNRAAVLDCVEVFEAAIDELH+SLGVLRRLSR
Sbjct: 67  AGVSVTITEAKKVAGLLGRLKNNKRMKGRNRAAVLDCVEVFEAAIDELHRSLGVLRRLSR 126

Query: 138 RNFDAQMGDLTTWVSAALTDEDTCLEGFEGERGKAVTLLRNRVVKVGYITS 188
           RNFDAQMGDLTTWVSAALTDEDTC+EGFEGE GK VTLLRNRVVK   I +
Sbjct: 127 RNFDAQMGDLTTWVSAALTDEDTCVEGFEGEEGKVVTLLRNRVVKAEKIAA 177

[Close]

BLAST of Chy1G016910 vs. NCBI nr
Match: XP_023514751.1 (pectinesterase inhibitor 6 [Cucurbita pepo subsp. pepo] >KAG7030218.1 Pectinesterase inhibitor 6, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 305 bits (781), Expect = 7.34e-103
Identity = 157/190 (82.63%), Postives = 166/190 (87.37%), Query Frame = 0


Query: 19  RASIAALLALISILPWLTHSAKTSYVQEACRVTRHQDLCIQSLSPFSSAAKRSPTKWARA 78
           R SI  LLA ISILPWLTHSA+TSYVQEAC VT H DLCI+SLSPFSS AKRSPTKWARA
Sbjct: 8   RTSIVTLLAFISILPWLTHSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKRSPTKWARA 67

Query: 79  GVSVTITEAKKVAGLLERLKNNKRMKGRNRAAVLDCVEVFEAAIDELHKSLGVLRRLSRR 138
            VSVTITEAK VA  L RLKNN+RMKGRNRAAV DCVEVFE AIDELH+SLGVLRRLSR 
Sbjct: 68  SVSVTITEAKAVARFLVRLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGVLRRLSRS 127

Query: 139 NFDAQMGDLTTWVSAALTDEDTCLEGFEGERGKAVTLLRNRVVKVGYITSNALALVNKLA 198
            FDAQMGDLTTWVSAALTDEDTC++GFEGERG+ V L+RNRVVK  YITSNALALVNKLA
Sbjct: 128 KFDAQMGDLTTWVSAALTDEDTCVDGFEGERGRLVNLIRNRVVKATYITSNALALVNKLA 187

Query: 199 ASSFETTINM 208
           AS FET  N+
Sbjct: 188 ASGFETAPNL 197

[Close]

BLAST of Chy1G016910 vs. TAIR 10
Match: AT3G62820.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 159.1 bits (401), Expect = 3.9e-39
Identity = 92/185 (49.73%), Postives = 122/185 (65.95%), Query Frame = 0


Query: 21  SIAALLALISILPWLT-HSAKTSYVQEACRVTRHQDLCIQSLSPFSSAAKRSPTKWARAG 80
           + A +  L+S+ P  +  S + SYVQ AC VTR+QDLC ++L PF+S AK SP+KWARAG
Sbjct: 10  TFALVFFLLSLNPTSSLPSKRESYVQNACSVTRYQDLCAKTLLPFASVAKNSPSKWARAG 69

Query: 81  VSVTITEAKKVAGLLERLKNNKRMKG-RNRAAVLDCVEVFEAAIDELHKSLGVLRRLSRR 140
           VSV IT+ K V  L   LK      G R+R A+ DC E+ + ++D LHKSL VLR L   
Sbjct: 70  VSVAITDNKDV--LRHLLKTRLSTIGKRDRIALSDCRELLQDSLDSLHKSLAVLRTLRAS 129

Query: 141 NFDAQMGDLTTWVSAALTDEDTCLEGFE---GERGKAVTLLRNRVVKVGYITSNALALVN 200
            F  QM DL TW+S++LTD+DTCL+GFE         V ++R RV    Y++SN+LAL+N
Sbjct: 130 EFQQQMSDLATWLSSSLTDKDTCLDGFEKTSTRSSSTVRMIRKRVTTSMYLSSNSLALLN 189

[Close]

BLAST of Chy1G016910 vs. TAIR 10
Match: AT2G47670.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 142.5 bits (358), Expect = 3.8e-34
Identity = 77/161 (47.83%), Postives = 109/161 (67.70%), Query Frame = 0


Query: 41  TSYVQEACRVTRHQDLCIQSLSPFSSAAKRSPTKWARAGVSVTITEAKKVAGLLERLKNN 100
           ++YV+ AC VTR+  LC+++L PF+  A+ + +KWARA V+VTIT+ K+V  LL  LK  
Sbjct: 46  STYVRNACNVTRYNRLCVRTLWPFAIVARNNTSKWARASVAVTITDTKRVLRLL--LKTQ 105

Query: 101 KRMKGRN-RAAVLDCVEVFEAAIDELHKSLGVLRRLSRRNFDAQMGDLTTWVSAALTDED 160
           +   G + R A+ DC E+F  ++D L+KSL VLR L+   F  Q+ DL TW+SAALTD+D
Sbjct: 106 RSAVGESERIALSDCRELFVDSLDNLYKSLAVLRTLNADEFQRQISDLATWLSAALTDDD 165

Query: 161 TCLEGFE--GERGKAVTLLRNRVVKVGYITSNALALVNKLA 199
           TCL+GFE    R + V ++R +  K   + SNALAL+ KLA
Sbjct: 166 TCLDGFEETSSRTRTVRMVRRKATKCMRLCSNALALLKKLA 204

[Close]

BLAST of Chy1G016910 vs. TAIR 10
Match: AT5G62360.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 128.6 bits (322), Expect = 5.7e-30
Identity = 71/182 (39.01%), Postives = 112/182 (61.54%), Query Frame = 0


Query: 22  IAALLALI--SILPWLTHSAKTSYVQEACRVTRHQDLCIQSLSPFSSAAKRSPTKWARAG 81
           I A+L L+  +     T +  T +V+ +C  T +  LC  SLS  +S  + SP   A A 
Sbjct: 20  IIAMLKLVHTTTTTTTTTTTNTEFVKSSCTFTTYPRLCFSSLSTHASLIQTSPKLMAHAA 79

Query: 82  VSVTITEAKKVAGLLERLKNNKRMKGRNRAAVLDCVEVFEAAIDELHKSLGVLRRLSRRN 141
           +++T+  AK  + ++ RL +N R+K +  +A+ DCVE     ++EL KS+G + +LS  N
Sbjct: 80  LNITLASAKVTSAMMVRL-SNSRLKPKEVSAMRDCVEELGDTLEELRKSIGEMCQLSGSN 139

Query: 142 FDAQMGDLTTWVSAALTDEDTCLEGFEGE--RGKAVTLLRNRVVKVGYITSNALALVNKL 200
           ++  + D+ TWVSAALTD +TC +GFEGE   GK   L+R R++ + ++TSNALAL+N  
Sbjct: 140 YEVYISDIQTWVSAALTDVNTCTDGFEGEDMDGKVKVLVRGRILVIAHLTSNALALINHF 199

[Close]

BLAST of Chy1G016910 vs. TAIR 10
Match: AT4G12390.1 (pectin methylesterase inhibitor 1 )

HSP 1 Score: 114.4 bits (285), Expect = 1.1e-25
Identity = 63/169 (37.28%), Postives = 103/169 (60.95%), Query Frame = 0


Query: 38  SAKTSYVQEACRVTRHQDLCIQSLSPFSSAAKRSPTKWARAGVSVTITEAKKVAGLLERL 97
           S+  +++  +CRVTR+Q LC++ L+ F+   +R+  + A+  ++VT+   +     + +L
Sbjct: 34  SSSINFIVSSCRVTRYQTLCVKCLAAFADKIRRNENQLAQTALAVTLVRVQSTTIYVGKL 93

Query: 98  KNNKRMKGRNRAAVLDCVEVFEAAIDELHKSLGVLRRLSRRNFDA-----QMGDLTTWVS 157
              +R+K R   AV DCVE     ++ L +S+  L+++ R   D      ++ ++ TWVS
Sbjct: 94  TKARRIKRREYLAVKDCVENLGDGLEMLAQSMRELKQVGRSGRDRDEFLWRLSNVETWVS 153

Query: 158 AALTDEDTCLEGFEGE--RGKAVTLLRNRVVKVGYITSNALALVNKLAA 200
           AALTDE TCL+GF+G+   G   + +R RVV V  +TSNALALVN+ AA
Sbjct: 154 AALTDETTCLDGFDGKVMDGVVKSAIRRRVVHVARVTSNALALVNRFAA 202

[Close]

BLAST of Chy1G016910 vs. TAIR 10
Match: AT5G20740.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 110.2 bits (274), Expect = 2.1e-24
Identity = 59/167 (35.33%), Postives = 101/167 (60.48%), Query Frame = 0


Query: 37  HSAKTSYVQEACRVTRHQDLCIQSLSPFSSAAKRSPTKWARAGVSVTITEAKKVAGLLER 96
           H+     V  +C    +  LC+++LS +S     +    A+A + ++++ A+  A  L  
Sbjct: 27  HNGAEDIVHSSCEHASYPSLCVRTLSSYSGPTITNRRDLAQAAIKISLSHAQSAAKKLAV 86

Query: 97  LKNNKRMKGRNRAAVLDCVEVFEAAIDELHKSLGVLRRL-----SRRNFDAQMGDLTTWV 156
           ++++   K + +AA++DCVE+   ++DEL ++LGVL+ L     S + F  QM +  TW 
Sbjct: 87  VRDSVGKKKQEKAALVDCVEMIGDSVDELSRTLGVLKHLRVSGGSAKEFRWQMSNAQTWA 146

Query: 157 SAALTDEDTCLEGFEG-ERGKAVTLLRNRVVKVGYITSNALALVNKL 198
           SAALTD+DTCL+GF+G + G+  T ++  + KV  +TSNAL +VN+L
Sbjct: 147 SAALTDDDTCLDGFQGMDDGEIKTEVKQWMTKVARVTSNALYMVNQL 193

The following BLAST results are available for this feature:

BLAST of Chy1G016910 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O22244	5.4e-33	47.83	Pectinesterase inhibitor 6 OS=Arabidopsis thaliana OX=3702 GN=PMEI6 PE=2 SV=1	[more]
P17407	2.0e-24	35.96	21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1	[more]
Q84WE4	3.0e-23	35.33	Pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PMEI3 PE=2 SV=1	[more]
Q9SB37	2.5e-22	36.31	Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1	[more]
Q9SI74	3.3e-22	33.53	Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1	[more]

BLAST of Chy1G016910 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KAX0	3.9e-95	96.86	PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G397050 PE=4 S...	[more]
A0A5D3D1R1	7.7e-91	92.63	21 kDa protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G00630...	[more]
A0A1S3BSW5	7.7e-91	92.63	21 kDa protein OS=Cucumis melo OX=3656 GN=LOC103492810 PE=4 SV=1	[more]
A0A6J1L076	8.8e-79	82.11	pectinesterase inhibitor 6 OS=Cucurbita maxima OX=3661 GN=LOC111497889 PE=4 SV=1	[more]
A0A6J1H9V5	2.0e-78	82.11	pectinesterase inhibitor 6-like OS=Cucurbita moschata OX=3662 GN=LOC111460929 PE...	[more]

BLAST of Chy1G016910 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011659361.1	6.94e-124	96.86	pectinesterase inhibitor 6 [Cucumis sativus]	[more]
XP_008451567.1	3.02e-118	92.63	PREDICTED: 21 kDa protein [Cucumis melo] >KAA0067994.1 21 kDa protein [Cucumis m...	[more]
XP_038896715.1	3.43e-108	87.30	pectinesterase inhibitor 6-like [Benincasa hispida]	[more]
KAE8646342.1	1.10e-104	93.57	hypothetical protein Csa_016554 [Cucumis sativus]	[more]
XP_023514751.1	7.34e-103	82.63	pectinesterase inhibitor 6 [Cucurbita pepo subsp. pepo] >KAG7030218.1 Pectineste...	[more]

BLAST of Chy1G016910 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G62820.1	3.9e-39	49.73	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT2G47670.1	3.8e-34	47.83	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G62360.1	5.7e-30	39.01	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT4G12390.1	1.1e-25	37.28	pectin methylesterase inhibitor 1	[more]
AT5G20740.1	2.1e-24	35.33	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 39..193 e-value: 5.0E-46 score: 168.9
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 43..193 e-value: 6.6E-33 score: 114.2
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 22..197 e-value: 1.5E-35 score: 120.1
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 38..199 e-value: 6.9E-36 score: 125.5
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 42..197
None	No IPR available	PANTHER	PTHR31080	PECTINESTERASE INHIBITOR-LIKE	coord: 31..192
None	No IPR available	PANTHER	PTHR31080:SF158	PLANT INVERTASE/PECTIN METHYLESTERASE INHIBITOR SUPERFAMILY PROTEIN	coord: 31..192
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 47..199 e-value: 4.57424E-46 score: 147.585

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy1G016910.1	Chy1G016910.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
molecular_function	GO:0004857	enzyme inhibitor activity