Chy1G016550 (gene) Cucumber (hystrix) v1

Overview
NameChy1G016550
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionPeptide chain release factor 1
LocationchrH01: 22477309 .. 22483808 (+)
RNA-Seq ExpressionChy1G016550
SyntenyChy1G016550
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGTATTTTCAATCCGACCACCGCAGCTCCGACGATTCCGAGCACTTTTCTTCTTCCGCCGCGACACAGTGGCTTATTCAGAAGATGCTCTCTGGAACTCAAACGCTTTGCACCTCCCTTTAGAGCTTCAACTCTTATTTGCATGGCGGTTTGTACAATTTTCGATTGATACATGCTCTTAGATTTACCGAATGTTTTGCTTTCGAGATTGGCTTTGAATGAACTTGAGTTTCGTTATGCTTTGAGTTCACTGAGTTTTCCTTCAAGTTATTAGAAATGAATTCGTGGTAAAACTAGTCTGGATGAGATTTGTGGCAAATGATGATGATGATTGCTTTATTTTAGGAACCTTATTTGATCGTGAAGCTGGAATCTGCTGAGAAGACGTGGAAAGAATTATCGGTAATTTGTGCTTTGCTGTTGAATGACATTCTTATTTCTTCACTGATTGGAGTCCATCAATTGTTTATATCCGGTGTTTATGCTTGGAATGGAACTGTTATTTTGCATTGAATTTGAGAGCCCGCGCCTGTGTTAACTACTGATGTATTCATCAAGTCGTTGGAAATGTAAGGGCATAAAGTGCAGAACATTGTTAACTATTGGATCAATGTTTCGGTTGTTGAAGTACAGTGGCTATCGAGCTGGAATAGGATAAGTAGCTAAGATATGTCTTGGAAAAGTCGAGCTCACAGAATCCTGCGGTGAAAGTTTAAGTTTTAAAATTAATAGATTAGGTGCTTGACGATTCCATTTAGCTACTTCTAGAGCTATAGCAAATAAGAAAAGAACGAAGAACTTCCTCACTAGAGACCTTGTCTATGCACTTGATATTGAAACTGGAAGACAAATACTGAATGCAATTTGGATTTGGTTATAGTGATCACCTTATAATTCCTTGCTTTCCTATTGCAGTTGGGATTTGGATATGACATTCTTTAGCTTTCACAATTAATTTTTAACCATTGCTTTCCTCCTGGAAGAAGACGAGGAACCGTGGTGTATCTGGAATCTCTATAGTAGTCGTATAACTAATGAAGTGATTATGCTTAAAATCTAGATAATGTTAACTGTATCTGAGTTTCCTCCCCTTTCATTCTCTAGATCCACTTTTGAAATTTGCATTTACATCTTTGATATATTTCCAATTATGCTTTTCATTTGGTTATAGCTTTAATATTTTCCTACACCAGTGTCATTATCGATATAATTATTTAGGTCAAGCTGGCCGATCCAGATATTGTCTCAAATCCCACAGAGTATCAAAAACTAGCCCAATCAATGTCAGAACTTGATGAGGTATGGCAATGCTGTCTGCCTCCAATACCATTATGTGTAACAGTTTGAATGACCTCTTTTATACATTCTTCCTTAAATAAGTGTCTTTCACACTAACTTACTCTTTGTTGGTTGGCGTGGGTTGCCTGGACAGGTGGTATCTACTTTCAGAAAATTCAAGGAATGTGAAAAGCAGTTAGAGGAGGCCAAAGGTTGGCTTATAAGTAGTTGTTCTTAAAAAGTTTCCCAACATAAAATGTGTTAAATTGATCTTAGTTTCTTCTTCTTTGTTGTGATTATCATGCCAGCCTTATCGAAAGAGAGTGGGAACGATGAGGATATGGTAGAGATGATTGCTTTAGAAATCAGTTCTTTATCCAGTGAGCTCAAAGATATCGAAGAGAAGCTTAAGGTGCTTTTGGTTCATGAACATGATTTTTTCATGTGTGCGTTTATTTTGGATCCAAACGAACTTTTATTAATGTTAACATGTATTTAAATCTTTTTCTTGTCATTCTGTTTCCATGTTTTGGGTGGGGTGGAGGTCCTTGATTTTCAATCCTCACAGTTCATGGTTTAAAATTTTTATTTTCTGCTTTTTAATGAGTTTCTTAATGACATACAAGTCTAAATGCGTTATTTGTTTGATTTTTTTTAGCTATATTCAGTAATGCTTATTATGTTATCTTCTTGTTAAAGTGAATCAATTAATTACTGGAACGAATTTGTAATGACATTATAGCTTGGTTCTTTATTGGTGTTTGGATCTTTACAAGCAAATATTGTGCTTGTCTTGTCATTCTTACAATGCTAATTTTCTTTTGTTGGGGACTTTATTTATTTCCTGGTATTCATCATTTGAATAAAAGGAAGTTATTTCCTGGTATCTCCTCACAAGTACATCTTATATTTTTCTGAGTTGAATCTGAAGTGCTGTGATTTTCTCTGTCAGGTGATGTTGCTTCCATCTGATCCTCTTGATGCAAGAAATATTATGCTTGAAGGTCAACTTCCTCATTTTCCTCTGAAATTTTATGTCATCTGAATAACAACAAACTTTAACGTACATCTGCTGAAGGTTGCATAGCTTATTTGACAATTAGAATCCTGTAAATGTAAATGGGTTATGTTTATGCCTCTTGTTTATTCTGATTAAAAGCTTTCCTTCTGCCATGCAGTCCGGGCAGGTACAGGTGGTGATGAAGCTGGAATCTGGGCTGGTGATCTTGTAGGTACATTTTCTTTTATCCTTAAAGAACTGTGGACACGTTTTTAATACATTATTATTGTTAGCATCCTTTTTAAAAAAAATTATTGGTAGTGTACCATACTGTTGGTGAACAATGGAAATATTGGTTTTAAAAGAAAATCTCTTGATACTGCTATGCTTAATTAATGCATTAGATGTAACCTATGCTCCAATCTTTTCTTACCAACAGTTTAGGCCTTTTAAGTGTATAGAAAACTGAATAGGGAATAGGATAGATAGGCTTTGTAAAACTTACAAGTTGCATAGTCTGAACTCTGATGTTTCTTCCGTTCTTGTCCTGCTTTGATTTTACCCCTTCTTTTCTCAAATGCATTTTTCTCTATTGTCGTTTCTTCTTCTCTAACTCGTTTTTTCCAATTATATAACAGGTCCGTATGTACCAGAAATACAGTGAGCGAAATTCTTGGAAGTTTTCCCCTATTTCAAGTTCTGAGGTACGTGTCTCCAGACATCTTAGAAGTCAATATCCTAGTGCTTTCTGTATATGTTGAAGAATTTCCGCTGTTTACTTTTCAGGCTGAAAAAGGTGGATACAAAACTTATGTGTTGGAAATCAAAGGACAATGTGTCTACAGTAAATTGAAATATGAGTCTGGTGTTCACCGGGTTCAACGTGTTCCTCAAACAGAAACACAGGGCCGTGTGCATACTTCTACTGCTACAGTCGCTATCATGCCAGAGGTAAACTATTCCATAAGTTCACTATTGAATTGTGAAATAGAATTGTCTGATACTTGTCAAATAAGAATTGCATGCCTTAATCAGACTAATGTGTGGTAGAATATTACCTGAATTTGCTAAGTTTGTCCTGGACAGTTGTCTAATGTTAATTCTTTTCTCCCTTTCATATATAGGTCTCTGATCTGCTATTGTGCTCCATTTTTGCCATTAACAATTATAATCCAAGTGCATAACTATATAGTTATCCTTATATATAGTTGATATTGATAGTGTTCATACTGTTTTTTTGTAGGAGTATTAACAATTTTGGATTTGATAATTTCTCTAATTTGACTTTGTTGTCCTCTGCATTCACATCTTGATCAGCTATATTTATATTGATTTGTTGAGCTTATGTTATTATTGCATCTAGGCAGATGAAGTTGAAGTAGTGATTGACCCGAAGGACATTGAACTAACTACTGCAAGATCTGGGGGTGCAGGAGGTATAACTTTACATAATTGATTCAAATATTGCTTTCAATTAGCTCTCTGAATCTGTTCTCATAAAACCAAGAAAAATGGTAGTATAACCTTTCTATTTATTTCTTGTTGTCATTAATTATGCAGGGCAGAATGTCAACAAGGTGGAGACAGCGATTGATCTTTTCCACAAGCCGACAGGAATCCGCATTTTTTGCACTGAAGAAAGGACTCAACTTCAGAATAAACTTCGAGCTTTTCAATTGCTGCGAGCCAAACTGTGAGCATCCATTACAATCATAACATATCTCACATCATCTTATAGATTATGTGAATGTGCACCTCATTCATATAATTTCTGTACTTTTCTTTTTCTGAGTACAAATTGGGGATGGGGGGATTTGAACCCGAGACCTCTTGGTTCTTAGGTACAGACATACAAGTGTTAGTTGAGCTTTGTTGGCAATTTCTATACTTTTTTGAGTAACAAATAATTGGAGAAAATTTGTGGCTGTATCTATGAGTTTTCTCAAAGAACTGAAGGATCTTTATTCCCATTTCTATATATCGTGGGAGTTTTTGCAGTCATTGATATGGGTGAAATAAAGAAAGTAACCGAACAAACTGATATTGGACAGTTGGAGTTCAGCCCTGCACGGTCCTTTGACAAAGCCCTTCCCTCACTCCTAGCCACCATCGGCCATTGTTCACACTGAACACAACCTAGTGGAACCCCAACCACCCTCTGCTTGGGTTAGCATGTCACTTCCTCGCCAGCCACCTCCACCCCAGCCTGTAGGTCAGTCAGTTCTTTTGTGTTATAAAAGAAAAAAGTCAGTTCTTTTGTTCTCCCCCAACTGACAGTCAGCTTCACGGCGACATCTATCCTAATCTACACTGACTTTCACCCTACCTTCTACCACAACCAGACTTTCATAGTTGACAGGTGGTAATTTTGCGGGACTCGACATTATGTAAATGCATGAATTTAATCACCCCTCCCTCCTCTTTTCCTTTTCAGTTATGAGATCAAAGTGAGGGAACAGCAAGAGAAGATCAGGAACCAACGAAAATTACAGGCAATTCTGTTTTTCTTTTCTTTCTAATTTTTTTAGCCTTTGGAATTTAGCAAAGAACTCTTGTATGATCTGCATTTAGACTGTCCTTGAGTTGCTTTCATCAGACAAGTTTATGAAGTTCTTGCTGTTGGTTTGTCATGATTTTGTTTTAATTTTCCTGGGAAACAAAGAGCTGTTACTCTTGATTTTTTTCGAGTATTTCCCAGTCTTAAGAGTAATTTATCACTAAGCACATGATTCCGTTGGTAACATGCAAGATACAATCTGAATGGAATATCAAATACCAGAAATCTTTTAGAATCCACGATCTTCTACCCAGTGGTCTTAGGTACACAAATACACTGTTCTACAGCATAATATTGATATTTTAGGAATATTTTCTCTCCTTTTTTAAGAATCTAGTTCATGATATCACTTAAAAGTCATAATGTATTTTTTTGTTTAATTTACAATGGGCTGCCTGTATAACTCTCAATCTTAAAGCATGTATTTGTCAGATCATGCAAGGCAATTATACAAGCAGATAATTATTCTTATAGAAGCAATCGACAATAAAATTATTTTTAAAATTTATTTCAGTGTTCTAATTACCTTGTTATCCTAATTCCTATCTTCTGTCGCCTTGCGTAGGTGTTGAAATTTGTTGTCGGAATCTGTTTGAATGATGAACTTCATGCATACAACTGCTTGTGGTTGTCACAACTTGAATCTGTTAAAAAGCAAATACCTGATAATAACTTCTCTCAATGTAATTTAATTGGTACTCAAATTCTTTTCATTAGTGTATTCCTAACTCTAAAAGCTCTGTTTCAGGTAGGAACAGGTGCTCGTGCTGAGAAAATTCGAACTTACAATTACAAGGTATGAGATTAGTTTGCTTTCTTACTTAAAAAGAGGAAAAGAAATGCAGCTCCTGTCATAAAACAAAGATGTTGAAGATTAGAAAATTTTCAATTTTCAAAAAGATAAAGTGCATGTAGACACAGTAAGCAATGGCCTTGAATACCATTTCGCTTTAGAACTAACTAAAAGATCAGATACTTCGTTACAAGTAGATTGTATCAGGAGTGCTTTTTAACGGTATTTTCGAATAATTAAAATCTAGGAAAACTTTTCATTCTTAGTAACTTTTTTATTCCAAGTAAAAATAATTTCTGAAGTAATAATTGATCATTAAAGAAAAAAGAACACCACACAAACAATGAAGAACAAACTTATGAGGATTATACAAAAGAGTTGTCATAATGTCACTAATGAAATTTTAGTCTGTTTGGATTGACTTCATTTTTTTTTTCAGTTAAGAACATCATTTTTTAAATAGTTAGAAAGTCTATCCAAATTTAACTTGTGAAACTACCCTTTCCCATCTTTATCTCAAGCCGGAAATGAAACAGCTAATCATAAGTAATAGAATTTGACCTCAATAGGCTGATGGTTTCACTAGAAGTCATTAACTTTTAGATTTTACTTATGTTAGGACAATAGGGTTACCGACCATCGGTTGAAGACAAACTTCGAGCTCACATCCTTTCTTGAAGGTGATATAGAGGATGCAGTTCAGGTAATAATATTCTCCGTGTTTTACCACTTTCTCATTTTAATCATCATCTTAGTGGAAACCAAAAAGAATCACATTCTCCCATTTTCCTAATCAAGGCATGTGTTACCATGGAACAAAAAGAACTCCTAGAAGAACTGGCTCAATCGGCTGTGGCTTCAGCCACATGA

mRNA sequence

ATGAGTATTTTCAATCCGACCACCGCAGCTCCGACGATTCCGAGCACTTTTCTTCTTCCGCCGCGACACAGTGGCTTATTCAGAAGATGCTCTCTGGAACTCAAACGCTTTGCACCTCCCTTTAGAGCTTCAACTCTTATTTGCATGGCGGAACCTTATTTGATCGTGAAGCTGGAATCTGCTGAGAAGACGTGGAAAGAATTATCGGTCAAGCTGGCCGATCCAGATATTGTCTCAAATCCCACAGAGTATCAAAAACTAGCCCAATCAATGTCAGAACTTGATGAGGTGGTATCTACTTTCAGAAAATTCAAGGAATGTGAAAAGCAGTTAGAGGAGGCCAAAGCCTTATCGAAAGAGAGTGGGAACGATGAGGATATGGTAGAGATGATTGCTTTAGAAATCAGTTCTTTATCCAGTGAGCTCAAAGATATCGAAGAGAAGCTTAAGGTGATGTTGCTTCCATCTGATCCTCTTGATGCAAGAAATATTATGCTTGAAGTCCGGGCAGGTACAGGTGGTGATGAAGCTGGAATCTGGGCTGGTGATCTTGTCCGTATGTACCAGAAATACAGTGAGCGAAATTCTTGGAAGTTTTCCCCTATTTCAAGTTCTGAGGCTGAAAAAGGTGGATACAAAACTTATGTGTTGGAAATCAAAGGACAATGTGTCTACAGTAAATTGAAATATGAGTCTGGTGTTCACCGGGTTCAACGTGTTCCTCAAACAGAAACACAGGGCCGTGTGCATACTTCTACTGCTACAGTCGCTATCATGCCAGAGGCAGATGAAGTTGAAGTAGTGATTGACCCGAAGGACATTGAACTAACTACTGCAAGATCTGGGGGTGCAGGAGGGCAGAATGTCAACAAGGTGGAGACAGCGATTGATCTTTTCCACAAGCCGACAGGAATCCGCATTTTTTGCACTGAAGAAAGGACTCAACTTCAGAATAAACTTCGAGCTTTTCAATTGCTGCGAGCCAAACTTTATGAGATCAAAGTGAGGGAACAGCAAGAGAAGATCAGGAACCAACGAAAATTACAGGTAGGAACAGGTGCTCGTGCTGAGAAAATTCGAACTTACAATTACAAGGACAATAGGGTTACCGACCATCGGTTGAAGACAAACTTCGAGCTCACATCCTTTCTTGAAGGTGATATAGAGGATGCAGTTCAGGCATGTGTTACCATGGAACAAAAAGAACTCCTAGAAGAACTGGCTCAATCGGCTGTGGCTTCAGCCACATGA

Coding sequence (CDS)

ATGAGTATTTTCAATCCGACCACCGCAGCTCCGACGATTCCGAGCACTTTTCTTCTTCCGCCGCGACACAGTGGCTTATTCAGAAGATGCTCTCTGGAACTCAAACGCTTTGCACCTCCCTTTAGAGCTTCAACTCTTATTTGCATGGCGGAACCTTATTTGATCGTGAAGCTGGAATCTGCTGAGAAGACGTGGAAAGAATTATCGGTCAAGCTGGCCGATCCAGATATTGTCTCAAATCCCACAGAGTATCAAAAACTAGCCCAATCAATGTCAGAACTTGATGAGGTGGTATCTACTTTCAGAAAATTCAAGGAATGTGAAAAGCAGTTAGAGGAGGCCAAAGCCTTATCGAAAGAGAGTGGGAACGATGAGGATATGGTAGAGATGATTGCTTTAGAAATCAGTTCTTTATCCAGTGAGCTCAAAGATATCGAAGAGAAGCTTAAGGTGATGTTGCTTCCATCTGATCCTCTTGATGCAAGAAATATTATGCTTGAAGTCCGGGCAGGTACAGGTGGTGATGAAGCTGGAATCTGGGCTGGTGATCTTGTCCGTATGTACCAGAAATACAGTGAGCGAAATTCTTGGAAGTTTTCCCCTATTTCAAGTTCTGAGGCTGAAAAAGGTGGATACAAAACTTATGTGTTGGAAATCAAAGGACAATGTGTCTACAGTAAATTGAAATATGAGTCTGGTGTTCACCGGGTTCAACGTGTTCCTCAAACAGAAACACAGGGCCGTGTGCATACTTCTACTGCTACAGTCGCTATCATGCCAGAGGCAGATGAAGTTGAAGTAGTGATTGACCCGAAGGACATTGAACTAACTACTGCAAGATCTGGGGGTGCAGGAGGGCAGAATGTCAACAAGGTGGAGACAGCGATTGATCTTTTCCACAAGCCGACAGGAATCCGCATTTTTTGCACTGAAGAAAGGACTCAACTTCAGAATAAACTTCGAGCTTTTCAATTGCTGCGAGCCAAACTTTATGAGATCAAAGTGAGGGAACAGCAAGAGAAGATCAGGAACCAACGAAAATTACAGGTAGGAACAGGTGCTCGTGCTGAGAAAATTCGAACTTACAATTACAAGGACAATAGGGTTACCGACCATCGGTTGAAGACAAACTTCGAGCTCACATCCTTTCTTGAAGGTGATATAGAGGATGCAGTTCAGGCATGTGTTACCATGGAACAAAAAGAACTCCTAGAAGAACTGGCTCAATCGGCTGTGGCTTCAGCCACATGA

Protein sequence

MSIFNPTTAAPTIPSTFLLPPRHSGLFRRCSLELKRFAPPFRASTLICMAEPYLIVKLESAEKTWKELSVKLADPDIVSNPTEYQKLAQSMSELDEVVSTFRKFKECEKQLEEAKALSKESGNDEDMVEMIALEISSLSSELKDIEEKLKVMLLPSDPLDARNIMLEVRAGTGGDEAGIWAGDLVRMYQKYSERNSWKFSPISSSEAEKGGYKTYVLEIKGQCVYSKLKYESGVHRVQRVPQTETQGRVHTSTATVAIMPEADEVEVVIDPKDIELTTARSGGAGGQNVNKVETAIDLFHKPTGIRIFCTEERTQLQNKLRAFQLLRAKLYEIKVREQQEKIRNQRKLQVGTGARAEKIRTYNYKDNRVTDHRLKTNFELTSFLEGDIEDAVQACVTMEQKELLEELAQSAVASAT*
Homology
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BLAST of Chy1G016550 vs. ExPASy Swiss-Prot
Match: Q8RX79 (Peptide chain release factor APG3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APG3 PE=2 SV=1)

HSP 1 Score: 599.0 bits (1543), Expect = 4.2e-170
Identity = 304/374 (81.28%), Postives = 344/374 (91.98%), Query Frame = 0 

Query: 42  RASTLICMAEPYLIVKLESAEKTWKELSVKLADPDIVSNPTEYQKLAQSMSELDEVVSTF 101
           R   L+CMAEPYLI K+ES EKTWKELSVKLADPD+VSN +EYQKLAQSMSELDEVV+ F
Sbjct: 45  RTRRLVCMAEPYLIRKMESVEKTWKELSVKLADPDVVSNQSEYQKLAQSMSELDEVVTVF 104

Query: 102 RKFKECEKQLEEAKALSKESGNDEDMVEMIALEISSLSSELKDIEEKLKVMLLPSDPLDA 161
           R+FK+CEKQL E+K L+KE+G+DEDM EMI  EI+SL+ E++++E++LKV+LLPSDPLDA
Sbjct: 105 RRFKDCEKQLLESKVLAKEAGDDEDMAEMIGSEINSLTKEIEELEKQLKVLLLPSDPLDA 164

Query: 162 RNIMLEVRAGTGGDEAGIWAGDLVRMYQKYSERNSWKFSPISSSEAEKGGYKTYVLEIKG 221
           RNI+LEVRAGTGGDEA IW GDLVRMYQ+YSER+SWKFS +S SEAE GGYKT V+EIKG
Sbjct: 165 RNILLEVRAGTGGDEAAIWTGDLVRMYQRYSERSSWKFSMVSCSEAEHGGYKTCVMEIKG 224

Query: 222 QCVYSKLKYESGVHRVQRVPQTETQGRVHTSTATVAIMPEADEVEVVIDPKDIELTTARS 281
             VYSKLKYESGVHRVQRVPQTETQGRVHTSTATVAIMPEADEVEVVIDPKDIELT+ARS
Sbjct: 225 NRVYSKLKYESGVHRVQRVPQTETQGRVHTSTATVAIMPEADEVEVVIDPKDIELTSARS 284

Query: 282 GGAGGQNVNKVETAIDLFHKPTGIRIFCTEERTQLQNKLRAFQLLRAKLYEIKVREQQEK 341
           GGAGGQNVNKVETAIDLFHKP+GIRIFCTEERTQ++NK RAFQLLRAKLYEIKVREQQEK
Sbjct: 285 GGAGGQNVNKVETAIDLFHKPSGIRIFCTEERTQIRNKARAFQLLRAKLYEIKVREQQEK 344

Query: 342 IRNQRKLQVGTGARAEKIRTYNYKDNRVTDHRLKTNFELTSFLEGDIEDAVQACVTMEQK 401
           IRN+RK QVGTGAR+EKIRTYNYKD+RVTDHRLK NF LT+FL+G +EDAVQAC  +EQK
Sbjct: 345 IRNERKSQVGTGARSEKIRTYNYKDSRVTDHRLKMNFALTTFLDGALEDAVQACAALEQK 404

Query: 402 ELLEELAQSAVASA 416
           EL+EEL++S  AS+
Sbjct: 405 ELMEELSESVAASS 418

[Close]
BLAST of Chy1G016550 vs. ExPASy Swiss-Prot
Match: Q110D7 (Peptide chain release factor 1 OS=Trichodesmium erythraeum (strain IMS101) OX=203124 GN=prfA PE=3 SV=1)

HSP 1 Score: 463.4 bits (1191), Expect = 2.7e-129
Identity = 233/363 (64.19%), Postives = 298/363 (82.09%), Query Frame = 0 

Query: 49  MAEPYLIVKLESAEKTWKELSVKLADPDIVSNPTEYQKLAQSMSELDEVVSTFRKFKECE 108
           MAE YL+ KL+S E+T+ EL+++LADPD+  +P+E+QKLA++ S L+EVV+ + ++K  +
Sbjct: 1   MAETYLLDKLKSVEQTYNELTLRLADPDVAKDPSEFQKLAKARSSLEEVVNCYVEWKNAQ 60

Query: 109 KQLEEAKALSKESGNDEDMVEMIALEISSLSSELKDIEEKLKVMLLPSDPLDARNIMLEV 168
           ++L +AK + KE+  D +M EM  +E+  L ++L+ +E ++K+ LLP DP D +NIMLE+
Sbjct: 61  EELADAKEILKEAVGDLEMQEMAKVEVEDLEAKLESLENQMKIALLPRDPNDDKNIMLEI 120

Query: 169 RAGTGGDEAGIWAGDLVRMYQKYSERNSWKFSPISSSEAEKGGYKTYVLEIKGQCVYSKL 228
           RAGTGGDEA IWAGDLVRMY +YSE  SWK S +S S A+ GG+K  +LEIKG  VYSKL
Sbjct: 121 RAGTGGDEASIWAGDLVRMYSRYSENQSWKVSLLSESLADMGGFKEAILEIKGDHVYSKL 180

Query: 229 KYESGVHRVQRVPQTETQGRVHTSTATVAIMPEADEVEVVIDPKDIELTTARSGGAGGQN 288
           K+E+GVHRVQRVP TE  GRVHTSTATVAIMPE D+VEV ID KDIEL+TARSGGAGGQN
Sbjct: 181 KFEAGVHRVQRVPVTEAGGRVHTSTATVAIMPEVDDVEVEIDQKDIELSTARSGGAGGQN 240

Query: 289 VNKVETAIDLFHKPTGIRIFCTEERTQLQNKLRAFQLLRAKLYEIKVREQQEKIRNQRKL 348
           VNKVETA+DLFHKPTGIRIFCT+ER+QLQN+ RA Q+LRAKLYEIK++EQQ ++ + R+ 
Sbjct: 241 VNKVETAVDLFHKPTGIRIFCTQERSQLQNRERAMQILRAKLYEIKLQEQQAEVSSIRRS 300

Query: 349 QVGTGARAEKIRTYNYKDNRVTDHRLKTNFELTSFLEGDIEDAVQACVTMEQKELLEELA 408
           QVGTG+R+EKIRTYNYKDNRVTDHRL  NF L   LEGDIE+ +QAC+T +Q+E L+ELA
Sbjct: 301 QVGTGSRSEKIRTYNYKDNRVTDHRLNQNFSLVPLLEGDIENVIQACITQDQQERLQELA 360

Query: 409 QSA 412
            S+
Sbjct: 361 ASS 363

[Close]
BLAST of Chy1G016550 vs. ExPASy Swiss-Prot
Match: B1XJH8 (Peptide chain release factor 1 OS=Synechococcus sp. (strain ATCC 27264 / PCC 7002 / PR-6) OX=32049 GN=prfA PE=3 SV=1)

HSP 1 Score: 463.0 bits (1190), Expect = 3.6e-129
Identity = 229/360 (63.61%), Postives = 294/360 (81.67%), Query Frame = 0 

Query: 49  MAEPYLIVKLESAEKTWKELSVKLADPDIVSNPTEYQKLAQSMSELDEVVSTFRKFKECE 108
           MAE YL+ KL+S E+T++E++ +LADPDI +NP E Q++A+  S L+E V+TF ++KE +
Sbjct: 1   MAEAYLLDKLKSVEQTFQEMTRRLADPDIATNPDELQRVAKIRSSLEETVNTFNQWKEAQ 60

Query: 109 KQLEEAKALSKESGNDEDMVEMIALEISSLSSELKDIEEKLKVMLLPSDPLDARNIMLEV 168
           ++L  AK + KES  D +M EM ALE+  L + +  +E +LK++LLP DP D +NIMLE+
Sbjct: 61  EELRGAKEILKESAGDPEMKEMAALEVEELENTIDTLENRLKILLLPKDPNDEKNIMLEI 120

Query: 169 RAGTGGDEAGIWAGDLVRMYQKYSERNSWKFSPISSSEAEKGGYKTYVLEIKGQCVYSKL 228
           RAGTGGDEA IWAGDL+RMY +Y+E  +W+   +S S A+ GG+K  +LEI+G+ VYSKL
Sbjct: 121 RAGTGGDEASIWAGDLLRMYSRYAEGQNWRVKLLSESAADMGGFKAVILEIQGEQVYSKL 180

Query: 229 KYESGVHRVQRVPQTETQGRVHTSTATVAIMPEADEVEVVIDPKDIELTTARSGGAGGQN 288
           KYE+GVHRVQRVP TE  GRVHTSTAT+A+MPE D+VEV IDPKDIE+TTARSGGAGGQN
Sbjct: 181 KYEAGVHRVQRVPVTEAGGRVHTSTATIAVMPEVDDVEVEIDPKDIEMTTARSGGAGGQN 240

Query: 289 VNKVETAIDLFHKPTGIRIFCTEERTQLQNKLRAFQLLRAKLYEIKVREQQEKIRNQRKL 348
           VNKVETA+DLFHKPTGIR+FCTEER+QLQN+ RA Q+LRAKLYE++++ QQE++ + RK 
Sbjct: 241 VNKVETAVDLFHKPTGIRVFCTEERSQLQNRERAMQILRAKLYEMELQRQQEEVSSMRKS 300

Query: 349 QVGTGARAEKIRTYNYKDNRVTDHRLKTNFELTSFLEGDIEDAVQACVTMEQKELLEELA 408
           QVGTGAR+EKIRTYNYKDNRVTDHRL  NF L   LEGDIED +QAC++ +Q+  LE+LA
Sbjct: 301 QVGTGARSEKIRTYNYKDNRVTDHRLGQNFSLAPALEGDIEDMLQACISQDQQAQLEQLA 360

[Close]
BLAST of Chy1G016550 vs. ExPASy Swiss-Prot
Match: Q8DMG9 (Peptide chain release factor 1 OS=Thermosynechococcus elongatus (strain BP-1) OX=197221 GN=prfA PE=3 SV=1)

HSP 1 Score: 462.2 bits (1188), Expect = 6.1e-129
Identity = 232/360 (64.44%), Postives = 293/360 (81.39%), Query Frame = 0 

Query: 49  MAEPYLIVKLESAEKTWKELSVKLADPDIVSNPTEYQKLAQSMSELDEVVSTFRKFKECE 108
           MAE YL+ KL + E+T+ EL+ +LADPD+  NPTE+QK+A+S + L+E V+ + +++   
Sbjct: 1   MAEAYLLEKLRTVEQTFTELTRRLADPDVAVNPTEFQKVARSRAALEETVNAYHEWQSLN 60

Query: 109 KQLEEAKALSKESGNDEDMVEMIALEISSLSSELKDIEEKLKVMLLPSDPLDARNIMLEV 168
           +QL +A+ L KE+ N+ ++ +M   EI+ LS  +  +EE+LK++LLP DP D +NIMLE+
Sbjct: 61  QQLVDARQLHKEAANEPELRQMAEEEIAHLSHRIAQLEERLKILLLPRDPNDEKNIMLEI 120

Query: 169 RAGTGGDEAGIWAGDLVRMYQKYSERNSWKFSPISSSEAEKGGYKTYVLEIKGQCVYSKL 228
           RAG GGDEAGIWAGDLVRMY +Y++   W+ + +S S AE GG+K  +LEIKG  VYSKL
Sbjct: 121 RAGAGGDEAGIWAGDLVRMYSRYAKTQGWQVTLVSESLAEMGGFKEAILEIKGDRVYSKL 180

Query: 229 KYESGVHRVQRVPQTETQGRVHTSTATVAIMPEADEVEVVIDPKDIELTTARSGGAGGQN 288
           KYESGVHRVQRVP TE  GR+HTSTATVA+MPE DEVEV IDPKDIELTTARSGGAGGQN
Sbjct: 181 KYESGVHRVQRVPATEASGRIHTSTATVAVMPEVDEVEVEIDPKDIELTTARSGGAGGQN 240

Query: 289 VNKVETAIDLFHKPTGIRIFCTEERTQLQNKLRAFQLLRAKLYEIKVREQQEKIRNQRKL 348
           VNKVETA+DL+HKPTGIRIFCTEER+QL+NK RAFQ+LRAKLYEIK+REQQE I + R+ 
Sbjct: 241 VNKVETAVDLYHKPTGIRIFCTEERSQLKNKERAFQILRAKLYEIKLREQQEAITSMRRS 300

Query: 349 QVGTGARAEKIRTYNYKDNRVTDHRLKTNFELTSFLEGDIEDAVQACVTMEQKELLEELA 408
           QVGTG R+EKIRTYNYKD+RVTDHRL  NF L+S LEGD+E  +QAC++ +Q+E L +LA
Sbjct: 301 QVGTGDRSEKIRTYNYKDDRVTDHRLGQNFSLSSVLEGDLEPIIQACISRDQQEQLAQLA 360

[Close]
BLAST of Chy1G016550 vs. ExPASy Swiss-Prot
Match: B7KI17 (Peptide chain release factor 1 OS=Gloeothece citriformis (strain PCC 7424) OX=65393 GN=prfA PE=3 SV=1)

HSP 1 Score: 461.8 bits (1187), Expect = 8.0e-129
Identity = 234/362 (64.64%), Postives = 293/362 (80.94%), Query Frame = 0 

Query: 49  MAEPYLIVKLESAEKTWKELSVKLADPDIVSNPTEYQKLAQSMSELDEVVSTFRKFKECE 108
           MAE YL+ KL+S E+T+KEL+ +LADPDI +NP E Q++A++ S L+E V+T+  +++ +
Sbjct: 1   MAEAYLLEKLKSVEQTYKELTRRLADPDIATNPDELQRVAKARSSLEETVNTYETWQKTK 60

Query: 109 KQLEEAKALSKESGNDEDMVEMIALEISSLSSELKDIEEKLKVMLLPSDPLDARNIMLEV 168
           +++  A+ + KE+G D +M EM ALE+  L  +L+ +E KLKV+LLP DP D +NIMLE+
Sbjct: 61  EEITGARQILKEAGGDSEMREMAALEVEELEEQLEQLENKLKVLLLPRDPNDDKNIMLEI 120

Query: 169 RAGTGGDEAGIWAGDLVRMYQKYSERNSWKFSPISSSEAEKGGYKTYVLEIKGQCVYSKL 228
           RAGTGGDEA IWAGDLVRMY +Y+E  +W    +S S A+ GG+K  +LEIKG  VYSKL
Sbjct: 121 RAGTGGDEASIWAGDLVRMYSRYAESQNWVVKLVSESLADMGGFKEAILEIKGDQVYSKL 180

Query: 229 KYESGVHRVQRVPQTETQGRVHTSTATVAIMPEADEVEVVIDPKDIELTTARSGGAGGQN 288
           K+E+GVHRVQRVP TE  GRVHTSTATVAIMPE DEVEV IDPKDIE++TARSGGAGGQN
Sbjct: 181 KFEAGVHRVQRVPVTEAGGRVHTSTATVAIMPEVDEVEVQIDPKDIEVSTARSGGAGGQN 240

Query: 289 VNKVETAIDLFHKPTGIRIFCTEERTQLQNKLRAFQLLRAKLYEIKVREQQEKIRNQRKL 348
           VNKVETA+DLFHKPTGIRIFCTEER+QLQN+ RA Q+LRAKLYE+K+REQQE + + R+ 
Sbjct: 241 VNKVETAVDLFHKPTGIRIFCTEERSQLQNRERAMQILRAKLYEMKLREQQEAVSSMRRS 300

Query: 349 QVGTGARAEKIRTYNYKDNRVTDHRLKTNFELTSFLEGDIEDAVQACVTMEQKELLEELA 408
           QVGTG+R+EKIRTYNYKDNRVTDHRL  NF L   LEGDIE  +Q+C+  +Q+E L ELA
Sbjct: 301 QVGTGSRSEKIRTYNYKDNRVTDHRLGQNFSLVGALEGDIEVIIQSCIGKDQQERLAELA 360

Query: 409 QS 411
            S
Sbjct: 361 NS 362

[Close]
BLAST of Chy1G016550 vs. ExPASy TrEMBL
Match: A0A0A0K944 (RF_PROK_I domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G394660 PE=3 SV=1)

HSP 1 Score: 772.7 bits (1994), Expect = 7.8e-220
Identity = 405/416 (97.36%), Postives = 410/416 (98.56%), Query Frame = 0 

Query: 1   MSIFNPTTAAPTIPSTFLLPPRHSGLFRRCSLELKRFAPPFRASTLICMAEPYLIVKLES 60
           MSIFNPTTAAPTIPSTFLLPPRHSGLFRRC+L+LKRFAPPFRASTLICMAEPYLIVKLES
Sbjct: 1   MSIFNPTTAAPTIPSTFLLPPRHSGLFRRCALQLKRFAPPFRASTLICMAEPYLIVKLES 60

Query: 61  AEKTWKELSVKLADPDIVSNPTEYQKLAQSMSELDEVVSTFRKFKECEKQLEEAKALSKE 120
           AEKTWKELSVKLADPDIVSNPTEYQKLAQS+SELDEVVSTFRKFKECEKQLEEA    K+
Sbjct: 61  AEKTWKELSVKLADPDIVSNPTEYQKLAQSISELDEVVSTFRKFKECEKQLEEA----KD 120

Query: 121 SGNDEDMVEMIALEISSLSSELKDIEEKLKVMLLPSDPLDARNIMLEVRAGTGGDEAGIW 180
           SGNDEDMVEMIALEISSLSSELKDIEEKLKVMLLPSDPLDARNIMLEVRAGTGGDEAGIW
Sbjct: 121 SGNDEDMVEMIALEISSLSSELKDIEEKLKVMLLPSDPLDARNIMLEVRAGTGGDEAGIW 180

Query: 181 AGDLVRMYQKYSERNSWKFSPISSSEAEKGGYKTYVLEIKGQCVYSKLKYESGVHRVQRV 240
           AGDLVRMYQKYSERNSWKFSP+SSSEAEKGGYKTYVLEIKGQ VYSKLKYESGVHRVQRV
Sbjct: 181 AGDLVRMYQKYSERNSWKFSPVSSSEAEKGGYKTYVLEIKGQRVYSKLKYESGVHRVQRV 240

Query: 241 PQTETQGRVHTSTATVAIMPEADEVEVVIDPKDIELTTARSGGAGGQNVNKVETAIDLFH 300
           PQTETQGRVHTSTATVAIMPEADEVEVVIDPKDIELT ARSGGAGGQNVNKVETAIDLFH
Sbjct: 241 PQTETQGRVHTSTATVAIMPEADEVEVVIDPKDIELTIARSGGAGGQNVNKVETAIDLFH 300

Query: 301 KPTGIRIFCTEERTQLQNKLRAFQLLRAKLYEIKVREQQEKIRNQRKLQVGTGARAEKIR 360
           KPTGIRIFCTEERTQLQNKLRAFQLLRAKLYEIKVREQQEKIRNQRKLQVGTGARAEKIR
Sbjct: 301 KPTGIRIFCTEERTQLQNKLRAFQLLRAKLYEIKVREQQEKIRNQRKLQVGTGARAEKIR 360

Query: 361 TYNYKDNRVTDHRLKTNFELTSFLEGDIEDAVQACVTMEQKELLEELAQSAVASAT 417
           TYNYKDNRVTDHRLKTNFELTSFLEGDIEDAVQACVTMEQKELLEELAQSAVASAT
Sbjct: 361 TYNYKDNRVTDHRLKTNFELTSFLEGDIEDAVQACVTMEQKELLEELAQSAVASAT 412

[Close]
BLAST of Chy1G016550 vs. ExPASy TrEMBL
Match: A0A5A7VLD6 (Peptide chain release factor APG3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G00120 PE=3 SV=1)

HSP 1 Score: 769.6 bits (1986), Expect = 6.6e-219
Identity = 400/416 (96.15%), Postives = 408/416 (98.08%), Query Frame = 0 

Query: 1   MSIFNPTTAAPTIPSTFLLPPRHSGLFRRCSLELKRFAPPFRASTLICMAEPYLIVKLES 60
           M+IF PTTAAPTIPS+FLLPP HSG F+RCSLELKRFAPP RASTLICMAEPYLIVKLES
Sbjct: 1   MNIFTPTTAAPTIPSSFLLPPPHSGFFKRCSLELKRFAPPSRASTLICMAEPYLIVKLES 60

Query: 61  AEKTWKELSVKLADPDIVSNPTEYQKLAQSMSELDEVVSTFRKFKECEKQLEEAKALSKE 120
           AEKTWKELSVKLADPD+VSNPTEYQKLAQSMSELDEVVSTFRKFKECE+QLEEAKALSKE
Sbjct: 61  AEKTWKELSVKLADPDVVSNPTEYQKLAQSMSELDEVVSTFRKFKECERQLEEAKALSKE 120

Query: 121 SGNDEDMVEMIALEISSLSSELKDIEEKLKVMLLPSDPLDARNIMLEVRAGTGGDEAGIW 180
           SGNDEDMVEMIALEISSLSSELKDIEEKLKVMLLPSDPLDARNIMLEVRAGTGGDEAGIW
Sbjct: 121 SGNDEDMVEMIALEISSLSSELKDIEEKLKVMLLPSDPLDARNIMLEVRAGTGGDEAGIW 180

Query: 181 AGDLVRMYQKYSERNSWKFSPISSSEAEKGGYKTYVLEIKGQCVYSKLKYESGVHRVQRV 240
           AGDLVRMYQKYSERNSWKFSP+SSSEAEKGGYKTYVLEIKGQ VYSKLKYESGVHRVQRV
Sbjct: 181 AGDLVRMYQKYSERNSWKFSPVSSSEAEKGGYKTYVLEIKGQRVYSKLKYESGVHRVQRV 240

Query: 241 PQTETQGRVHTSTATVAIMPEADEVEVVIDPKDIELTTARSGGAGGQNVNKVETAIDLFH 300
           PQTETQGRVHTSTATVAIMPEADEVE+VIDPKDIELTTARSGGAGGQNVNKVETAIDLFH
Sbjct: 241 PQTETQGRVHTSTATVAIMPEADEVEIVIDPKDIELTTARSGGAGGQNVNKVETAIDLFH 300

Query: 301 KPTGIRIFCTEERTQLQNKLRAFQLLRAKLYEIKVREQQEKIRNQRKLQVGTGARAEKIR 360
           KPTGIRIFCTEERTQLQNK RAFQLLRAKLYEIKVREQQEKIRNQRKLQVGTGARAEKIR
Sbjct: 301 KPTGIRIFCTEERTQLQNKHRAFQLLRAKLYEIKVREQQEKIRNQRKLQVGTGARAEKIR 360

Query: 361 TYNYKDNRVTDHRLKTNFELTSFLEGDIEDAVQACVTMEQKELLEELAQSAVASAT 417
           TYNYKDNRVTDHRLKTNFELTSFL+GDIEDAVQAC  MEQKELLEELAQSAVASAT
Sbjct: 361 TYNYKDNRVTDHRLKTNFELTSFLDGDIEDAVQACTAMEQKELLEELAQSAVASAT 416

[Close]
BLAST of Chy1G016550 vs. ExPASy TrEMBL
Match: A0A1S3BRZ7 (peptide chain release factor APG3, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103492855 PE=3 SV=1)

HSP 1 Score: 769.6 bits (1986), Expect = 6.6e-219
Identity = 400/416 (96.15%), Postives = 408/416 (98.08%), Query Frame = 0 

Query: 1   MSIFNPTTAAPTIPSTFLLPPRHSGLFRRCSLELKRFAPPFRASTLICMAEPYLIVKLES 60
           M+IF PTTAAPTIPS+FLLPP HSG F+RCSLELKRFAPP RASTLICMAEPYLIVKLES
Sbjct: 1   MNIFTPTTAAPTIPSSFLLPPPHSGFFKRCSLELKRFAPPSRASTLICMAEPYLIVKLES 60

Query: 61  AEKTWKELSVKLADPDIVSNPTEYQKLAQSMSELDEVVSTFRKFKECEKQLEEAKALSKE 120
           AEKTWKELSVKLADPD+VSNPTEYQKLAQSMSELDEVVSTFRKFKECE+QLEEAKALSKE
Sbjct: 61  AEKTWKELSVKLADPDVVSNPTEYQKLAQSMSELDEVVSTFRKFKECERQLEEAKALSKE 120

Query: 121 SGNDEDMVEMIALEISSLSSELKDIEEKLKVMLLPSDPLDARNIMLEVRAGTGGDEAGIW 180
           SGNDEDMVEMIALEISSLSSELKDIEEKLKVMLLPSDPLDARNIMLEVRAGTGGDEAGIW
Sbjct: 121 SGNDEDMVEMIALEISSLSSELKDIEEKLKVMLLPSDPLDARNIMLEVRAGTGGDEAGIW 180

Query: 181 AGDLVRMYQKYSERNSWKFSPISSSEAEKGGYKTYVLEIKGQCVYSKLKYESGVHRVQRV 240
           AGDLVRMYQKYSERNSWKFSP+SSSEAEKGGYKTYVLEIKGQ VYSKLKYESGVHRVQRV
Sbjct: 181 AGDLVRMYQKYSERNSWKFSPVSSSEAEKGGYKTYVLEIKGQRVYSKLKYESGVHRVQRV 240

Query: 241 PQTETQGRVHTSTATVAIMPEADEVEVVIDPKDIELTTARSGGAGGQNVNKVETAIDLFH 300
           PQTETQGRVHTSTATVAIMPEADEVE+VIDPKDIELTTARSGGAGGQNVNKVETAIDLFH
Sbjct: 241 PQTETQGRVHTSTATVAIMPEADEVEIVIDPKDIELTTARSGGAGGQNVNKVETAIDLFH 300

Query: 301 KPTGIRIFCTEERTQLQNKLRAFQLLRAKLYEIKVREQQEKIRNQRKLQVGTGARAEKIR 360
           KPTGIRIFCTEERTQLQNK RAFQLLRAKLYEIKVREQQEKIRNQRKLQVGTGARAEKIR
Sbjct: 301 KPTGIRIFCTEERTQLQNKHRAFQLLRAKLYEIKVREQQEKIRNQRKLQVGTGARAEKIR 360

Query: 361 TYNYKDNRVTDHRLKTNFELTSFLEGDIEDAVQACVTMEQKELLEELAQSAVASAT 417
           TYNYKDNRVTDHRLKTNFELTSFL+GDIEDAVQAC  MEQKELLEELAQSAVASAT
Sbjct: 361 TYNYKDNRVTDHRLKTNFELTSFLDGDIEDAVQACTAMEQKELLEELAQSAVASAT 416

[Close]
BLAST of Chy1G016550 vs. ExPASy TrEMBL
Match: A0A6J1DC40 (peptide chain release factor APG3, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111019348 PE=3 SV=1)

HSP 1 Score: 735.7 bits (1898), Expect = 1.1e-208
Identity = 384/416 (92.31%), Postives = 395/416 (94.95%), Query Frame = 0 

Query: 1   MSIFNPTTAAPTIPSTFLLPPRHSGLFRRCSLELKRFAPPFRASTLICMAEPYLIVKLES 60
           M+IF+PT+AA T P  FLLPPRHS   RRCSLE KR AP +RAST+ICMAEPYLIVKLES
Sbjct: 1   MNIFSPTSAARTCPGCFLLPPRHSDFSRRCSLEFKRSAPLYRASTVICMAEPYLIVKLES 60

Query: 61  AEKTWKELSVKLADPDIVSNPTEYQKLAQSMSELDEVVSTFRKFKECEKQLEEAKALSKE 120
           AEKTWKELSVKLADPDIVSNPTEYQK AQSMSELDEVVSTFR FKECEKQLEE KAL+KE
Sbjct: 61  AEKTWKELSVKLADPDIVSNPTEYQKFAQSMSELDEVVSTFRNFKECEKQLEETKALAKE 120

Query: 121 SGNDEDMVEMIALEISSLSSELKDIEEKLKVMLLPSDPLDARNIMLEVRAGTGGDEAGIW 180
           SGNDEDM EMIALEI+SLSS+LKD+EEKLKVMLLPSDPLDARNIMLEVRAGTGGDEAGIW
Sbjct: 121 SGNDEDMAEMIALEINSLSSQLKDLEEKLKVMLLPSDPLDARNIMLEVRAGTGGDEAGIW 180

Query: 181 AGDLVRMYQKYSERNSWKFSPISSSEAEKGGYKTYVLEIKGQCVYSKLKYESGVHRVQRV 240
           AGDLVRMYQKYSERNSWKFSPISSSEAEKGG+KTYVLEIKG CVYSKLKYESGVHRVQRV
Sbjct: 181 AGDLVRMYQKYSERNSWKFSPISSSEAEKGGFKTYVLEIKGNCVYSKLKYESGVHRVQRV 240

Query: 241 PQTETQGRVHTSTATVAIMPEADEVEVVIDPKDIELTTARSGGAGGQNVNKVETAIDLFH 300
           PQTETQGRVHTSTATVAIMPEADEVEVVIDPKDIEL TARSGGAGGQNVNKVETAIDLFH
Sbjct: 241 PQTETQGRVHTSTATVAIMPEADEVEVVIDPKDIELQTARSGGAGGQNVNKVETAIDLFH 300

Query: 301 KPTGIRIFCTEERTQLQNKLRAFQLLRAKLYEIKVREQQEKIRNQRKLQVGTGARAEKIR 360
           KPTGIRIFCTEERTQLQNK RAFQLLRAKLYEIKVREQQE IRNQRKLQVGTGARAEKIR
Sbjct: 301 KPTGIRIFCTEERTQLQNKHRAFQLLRAKLYEIKVREQQELIRNQRKLQVGTGARAEKIR 360

Query: 361 TYNYKDNRVTDHRLKTNFELTSFLEGDIEDAVQACVTMEQKELLEELAQSAVASAT 417
           TYNYKDNRVTDHRLK NFELTSFLEGDIE+AVQAC  MEQKELLEELAQSAVASAT
Sbjct: 361 TYNYKDNRVTDHRLKMNFELTSFLEGDIENAVQACAAMEQKELLEELAQSAVASAT 416

[Close]
BLAST of Chy1G016550 vs. ExPASy TrEMBL
Match: A0A6J1KZW8 (peptide chain release factor APG3, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111497823 PE=3 SV=1)

HSP 1 Score: 726.5 bits (1874), Expect = 6.4e-206
Identity = 377/416 (90.62%), Postives = 391/416 (93.99%), Query Frame = 0 

Query: 1   MSIFNPTTAAPTIPSTFLLPPRHSGLFRRCSLELKRFAPPFRASTLICMAEPYLIVKLES 60
           M+ F+PTTA  TIP  FL PPR+    RRCSLELKR APPFRASTL+CMAEPYLI KLES
Sbjct: 1   MNFFSPTTATRTIPGCFLFPPRYGDFSRRCSLELKRSAPPFRASTLVCMAEPYLITKLES 60

Query: 61  AEKTWKELSVKLADPDIVSNPTEYQKLAQSMSELDEVVSTFRKFKECEKQLEEAKALSKE 120
           AE TWKELSVKLADPDIVSNPTEYQKLAQSMSELDEVVSTFR+FKECEKQL+EAKAL+KE
Sbjct: 61  AENTWKELSVKLADPDIVSNPTEYQKLAQSMSELDEVVSTFRRFKECEKQLQEAKALAKE 120

Query: 121 SGNDEDMVEMIALEISSLSSELKDIEEKLKVMLLPSDPLDARNIMLEVRAGTGGDEAGIW 180
           SGNDEDM EMIALEISSLSS+LKDIEEKLKVMLLPSDPLDARNIMLEVRAGTGGDEAGIW
Sbjct: 121 SGNDEDMAEMIALEISSLSSQLKDIEEKLKVMLLPSDPLDARNIMLEVRAGTGGDEAGIW 180

Query: 181 AGDLVRMYQKYSERNSWKFSPISSSEAEKGGYKTYVLEIKGQCVYSKLKYESGVHRVQRV 240
           AGDLVRMYQKYSERNSWKFSPISSSEAEKGG+KTYVLEIKG  VYSKLKYESGVHRVQRV
Sbjct: 181 AGDLVRMYQKYSERNSWKFSPISSSEAEKGGFKTYVLEIKGNRVYSKLKYESGVHRVQRV 240

Query: 241 PQTETQGRVHTSTATVAIMPEADEVEVVIDPKDIELTTARSGGAGGQNVNKVETAIDLFH 300
           PQTETQGRVHTSTATVAIMPEADEVEVVIDPKDIELTT RSGGAGGQNVNKVETAIDLFH
Sbjct: 241 PQTETQGRVHTSTATVAIMPEADEVEVVIDPKDIELTTTRSGGAGGQNVNKVETAIDLFH 300

Query: 301 KPTGIRIFCTEERTQLQNKLRAFQLLRAKLYEIKVREQQEKIRNQRKLQVGTGARAEKIR 360
           KPTGIRIFCTEERTQLQNK RAFQLLRAKLYEIKVREQQE+IRNQRKLQVGTGARAEKIR
Sbjct: 301 KPTGIRIFCTEERTQLQNKHRAFQLLRAKLYEIKVREQQERIRNQRKLQVGTGARAEKIR 360

Query: 361 TYNYKDNRVTDHRLKTNFELTSFLEGDIEDAVQACVTMEQKELLEELAQSAVASAT 417
           TYNYKDNRVTDHRLK NFELTSF++GDIE+AVQACV MEQKEL+EEL QS  A  T
Sbjct: 361 TYNYKDNRVTDHRLKMNFELTSFIDGDIENAVQACVAMEQKELMEELVQSTGAPTT 416

[Close]
BLAST of Chy1G016550 vs. NCBI nr
Match: XP_031745234.1 (peptide chain release factor APG3, chloroplastic [Cucumis sativus] >KGN45452.2 hypothetical protein Csa_016721 [Cucumis sativus])

HSP 1 Score: 790 bits (2041), Expect = 9.55e-288
Identity = 412/416 (99.04%), Postives = 415/416 (99.76%), Query Frame = 0 

Query: 1   MSIFNPTTAAPTIPSTFLLPPRHSGLFRRCSLELKRFAPPFRASTLICMAEPYLIVKLES 60
           MSIFNPTTAAPTIPSTFLLPPRHSGLFRRC+L+LKRFAPPFRASTLICMAEPYLIVKLES
Sbjct: 1   MSIFNPTTAAPTIPSTFLLPPRHSGLFRRCALQLKRFAPPFRASTLICMAEPYLIVKLES 60

Query: 61  AEKTWKELSVKLADPDIVSNPTEYQKLAQSMSELDEVVSTFRKFKECEKQLEEAKALSKE 120
           AEKTWKELSVKLADPDIVSNPTEYQKLAQSMSELDEVVSTFRKFKECEKQLEEAKALSKE
Sbjct: 61  AEKTWKELSVKLADPDIVSNPTEYQKLAQSMSELDEVVSTFRKFKECEKQLEEAKALSKE 120

Query: 121 SGNDEDMVEMIALEISSLSSELKDIEEKLKVMLLPSDPLDARNIMLEVRAGTGGDEAGIW 180
           SGNDEDMVEMIALEISSLSSELKDIEEKLKVMLLPSDPLDARNIMLEVRAGTGGDEAGIW
Sbjct: 121 SGNDEDMVEMIALEISSLSSELKDIEEKLKVMLLPSDPLDARNIMLEVRAGTGGDEAGIW 180

Query: 181 AGDLVRMYQKYSERNSWKFSPISSSEAEKGGYKTYVLEIKGQCVYSKLKYESGVHRVQRV 240
           AGDLVRMYQKYSERNSWKFSP+SSSEAEKGGYKTYVLEIKGQ VYSKLKYESGVHRVQRV
Sbjct: 181 AGDLVRMYQKYSERNSWKFSPVSSSEAEKGGYKTYVLEIKGQRVYSKLKYESGVHRVQRV 240

Query: 241 PQTETQGRVHTSTATVAIMPEADEVEVVIDPKDIELTTARSGGAGGQNVNKVETAIDLFH 300
           PQTETQGRVHTSTATVAIMPEADEVEVVIDPKDIELTTARSGGAGGQNVNKVETAIDLFH
Sbjct: 241 PQTETQGRVHTSTATVAIMPEADEVEVVIDPKDIELTTARSGGAGGQNVNKVETAIDLFH 300

Query: 301 KPTGIRIFCTEERTQLQNKLRAFQLLRAKLYEIKVREQQEKIRNQRKLQVGTGARAEKIR 360
           KPTGIRIFCTEERTQLQNKLRAFQLLRAKLYEIKVREQQEKIRNQRKLQVGTGARAEKIR
Sbjct: 301 KPTGIRIFCTEERTQLQNKLRAFQLLRAKLYEIKVREQQEKIRNQRKLQVGTGARAEKIR 360

Query: 361 TYNYKDNRVTDHRLKTNFELTSFLEGDIEDAVQACVTMEQKELLEELAQSAVASAT 416
           TYNYKDNRVTDHRLKTNFELTSFLEGDIEDAVQACVTMEQKELLEELAQSAVASAT
Sbjct: 361 TYNYKDNRVTDHRLKTNFELTSFLEGDIEDAVQACVTMEQKELLEELAQSAVASAT 416

[Close]
BLAST of Chy1G016550 vs. NCBI nr
Match: XP_008451621.1 (PREDICTED: peptide chain release factor APG3, chloroplastic [Cucumis melo] >KAA0068037.1 peptide chain release factor APG3 [Cucumis melo var. makuwa] >TYK18091.1 peptide chain release factor APG3 [Cucumis melo var. makuwa])

HSP 1 Score: 770 bits (1988), Expect = 1.14e-279
Identity = 400/416 (96.15%), Postives = 408/416 (98.08%), Query Frame = 0 

Query: 1   MSIFNPTTAAPTIPSTFLLPPRHSGLFRRCSLELKRFAPPFRASTLICMAEPYLIVKLES 60
           M+IF PTTAAPTIPS+FLLPP HSG F+RCSLELKRFAPP RASTLICMAEPYLIVKLES
Sbjct: 1   MNIFTPTTAAPTIPSSFLLPPPHSGFFKRCSLELKRFAPPSRASTLICMAEPYLIVKLES 60

Query: 61  AEKTWKELSVKLADPDIVSNPTEYQKLAQSMSELDEVVSTFRKFKECEKQLEEAKALSKE 120
           AEKTWKELSVKLADPD+VSNPTEYQKLAQSMSELDEVVSTFRKFKECE+QLEEAKALSKE
Sbjct: 61  AEKTWKELSVKLADPDVVSNPTEYQKLAQSMSELDEVVSTFRKFKECERQLEEAKALSKE 120

Query: 121 SGNDEDMVEMIALEISSLSSELKDIEEKLKVMLLPSDPLDARNIMLEVRAGTGGDEAGIW 180
           SGNDEDMVEMIALEISSLSSELKDIEEKLKVMLLPSDPLDARNIMLEVRAGTGGDEAGIW
Sbjct: 121 SGNDEDMVEMIALEISSLSSELKDIEEKLKVMLLPSDPLDARNIMLEVRAGTGGDEAGIW 180

Query: 181 AGDLVRMYQKYSERNSWKFSPISSSEAEKGGYKTYVLEIKGQCVYSKLKYESGVHRVQRV 240
           AGDLVRMYQKYSERNSWKFSP+SSSEAEKGGYKTYVLEIKGQ VYSKLKYESGVHRVQRV
Sbjct: 181 AGDLVRMYQKYSERNSWKFSPVSSSEAEKGGYKTYVLEIKGQRVYSKLKYESGVHRVQRV 240

Query: 241 PQTETQGRVHTSTATVAIMPEADEVEVVIDPKDIELTTARSGGAGGQNVNKVETAIDLFH 300
           PQTETQGRVHTSTATVAIMPEADEVE+VIDPKDIELTTARSGGAGGQNVNKVETAIDLFH
Sbjct: 241 PQTETQGRVHTSTATVAIMPEADEVEIVIDPKDIELTTARSGGAGGQNVNKVETAIDLFH 300

Query: 301 KPTGIRIFCTEERTQLQNKLRAFQLLRAKLYEIKVREQQEKIRNQRKLQVGTGARAEKIR 360
           KPTGIRIFCTEERTQLQNK RAFQLLRAKLYEIKVREQQEKIRNQRKLQVGTGARAEKIR
Sbjct: 301 KPTGIRIFCTEERTQLQNKHRAFQLLRAKLYEIKVREQQEKIRNQRKLQVGTGARAEKIR 360

Query: 361 TYNYKDNRVTDHRLKTNFELTSFLEGDIEDAVQACVTMEQKELLEELAQSAVASAT 416
           TYNYKDNRVTDHRLKTNFELTSFL+GDIEDAVQAC  MEQKELLEELAQSAVASAT
Sbjct: 361 TYNYKDNRVTDHRLKTNFELTSFLDGDIEDAVQACTAMEQKELLEELAQSAVASAT 416

[Close]
BLAST of Chy1G016550 vs. NCBI nr
Match: XP_038876731.1 (peptide chain release factor APG3, chloroplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 751 bits (1940), Expect = 2.35e-272
Identity = 393/416 (94.47%), Postives = 399/416 (95.91%), Query Frame = 0 

Query: 1   MSIFNPTTAAPTIPSTFLLPPRHSGLFRRCSLELKRFAPPFRASTLICMAEPYLIVKLES 60
           M+IF PT+AAP IPS F LPPRHS    RCSLE KRFAPPFRASTLICMAEPYLIVKLES
Sbjct: 1   MNIFTPTSAAPAIPSCFFLPPRHSDFSGRCSLEFKRFAPPFRASTLICMAEPYLIVKLES 60

Query: 61  AEKTWKELSVKLADPDIVSNPTEYQKLAQSMSELDEVVSTFRKFKECEKQLEEAKALSKE 120
           AEKTWKELSVKLADPDIVSNPTEYQKLAQSMSELDEVV TFRKFKECEKQLEEAKALSKE
Sbjct: 61  AEKTWKELSVKLADPDIVSNPTEYQKLAQSMSELDEVVLTFRKFKECEKQLEEAKALSKE 120

Query: 121 SGNDEDMVEMIALEISSLSSELKDIEEKLKVMLLPSDPLDARNIMLEVRAGTGGDEAGIW 180
           SGNDEDMVEMIALEISSLSS+LKDIEE LKVMLLPSDPLDARNIMLEVRAGTGGDEAGIW
Sbjct: 121 SGNDEDMVEMIALEISSLSSQLKDIEENLKVMLLPSDPLDARNIMLEVRAGTGGDEAGIW 180

Query: 181 AGDLVRMYQKYSERNSWKFSPISSSEAEKGGYKTYVLEIKGQCVYSKLKYESGVHRVQRV 240
           AGDLVRMYQKYSERNSWKFSP+SSSEAEKGG+KTYVLEIKGQ VYSKLKYESGVHRVQRV
Sbjct: 181 AGDLVRMYQKYSERNSWKFSPVSSSEAEKGGFKTYVLEIKGQRVYSKLKYESGVHRVQRV 240

Query: 241 PQTETQGRVHTSTATVAIMPEADEVEVVIDPKDIELTTARSGGAGGQNVNKVETAIDLFH 300
           PQTETQGRVHTSTATVAIMPEADEVEVVIDPKDIELTTARSGGAGGQNVNKVETAIDLFH
Sbjct: 241 PQTETQGRVHTSTATVAIMPEADEVEVVIDPKDIELTTARSGGAGGQNVNKVETAIDLFH 300

Query: 301 KPTGIRIFCTEERTQLQNKLRAFQLLRAKLYEIKVREQQEKIRNQRKLQVGTGARAEKIR 360
           KPTGIRIFCTEERTQLQNK RAFQLLRAKLYEIKVREQQEKIRNQRKLQVGTGARAEKIR
Sbjct: 301 KPTGIRIFCTEERTQLQNKHRAFQLLRAKLYEIKVREQQEKIRNQRKLQVGTGARAEKIR 360

Query: 361 TYNYKDNRVTDHRLKTNFELTSFLEGDIEDAVQACVTMEQKELLEELAQSAVASAT 416
           TYNYKDNRVTDHRLK NFELTSFLEGDIE+AVQAC  MEQKELLEELAQSAVAS T
Sbjct: 361 TYNYKDNRVTDHRLKMNFELTSFLEGDIENAVQACAAMEQKELLEELAQSAVASTT 416

[Close]
BLAST of Chy1G016550 vs. NCBI nr
Match: XP_022151413.1 (peptide chain release factor APG3, chloroplastic [Momordica charantia])

HSP 1 Score: 736 bits (1900), Expect = 2.92e-266
Identity = 384/416 (92.31%), Postives = 395/416 (94.95%), Query Frame = 0 

Query: 1   MSIFNPTTAAPTIPSTFLLPPRHSGLFRRCSLELKRFAPPFRASTLICMAEPYLIVKLES 60
           M+IF+PT+AA T P  FLLPPRHS   RRCSLE KR AP +RAST+ICMAEPYLIVKLES
Sbjct: 1   MNIFSPTSAARTCPGCFLLPPRHSDFSRRCSLEFKRSAPLYRASTVICMAEPYLIVKLES 60

Query: 61  AEKTWKELSVKLADPDIVSNPTEYQKLAQSMSELDEVVSTFRKFKECEKQLEEAKALSKE 120
           AEKTWKELSVKLADPDIVSNPTEYQK AQSMSELDEVVSTFR FKECEKQLEE KAL+KE
Sbjct: 61  AEKTWKELSVKLADPDIVSNPTEYQKFAQSMSELDEVVSTFRNFKECEKQLEETKALAKE 120

Query: 121 SGNDEDMVEMIALEISSLSSELKDIEEKLKVMLLPSDPLDARNIMLEVRAGTGGDEAGIW 180
           SGNDEDM EMIALEI+SLSS+LKD+EEKLKVMLLPSDPLDARNIMLEVRAGTGGDEAGIW
Sbjct: 121 SGNDEDMAEMIALEINSLSSQLKDLEEKLKVMLLPSDPLDARNIMLEVRAGTGGDEAGIW 180

Query: 181 AGDLVRMYQKYSERNSWKFSPISSSEAEKGGYKTYVLEIKGQCVYSKLKYESGVHRVQRV 240
           AGDLVRMYQKYSERNSWKFSPISSSEAEKGG+KTYVLEIKG CVYSKLKYESGVHRVQRV
Sbjct: 181 AGDLVRMYQKYSERNSWKFSPISSSEAEKGGFKTYVLEIKGNCVYSKLKYESGVHRVQRV 240

Query: 241 PQTETQGRVHTSTATVAIMPEADEVEVVIDPKDIELTTARSGGAGGQNVNKVETAIDLFH 300
           PQTETQGRVHTSTATVAIMPEADEVEVVIDPKDIEL TARSGGAGGQNVNKVETAIDLFH
Sbjct: 241 PQTETQGRVHTSTATVAIMPEADEVEVVIDPKDIELQTARSGGAGGQNVNKVETAIDLFH 300

Query: 301 KPTGIRIFCTEERTQLQNKLRAFQLLRAKLYEIKVREQQEKIRNQRKLQVGTGARAEKIR 360
           KPTGIRIFCTEERTQLQNK RAFQLLRAKLYEIKVREQQE IRNQRKLQVGTGARAEKIR
Sbjct: 301 KPTGIRIFCTEERTQLQNKHRAFQLLRAKLYEIKVREQQELIRNQRKLQVGTGARAEKIR 360

Query: 361 TYNYKDNRVTDHRLKTNFELTSFLEGDIEDAVQACVTMEQKELLEELAQSAVASAT 416
           TYNYKDNRVTDHRLK NFELTSFLEGDIE+AVQAC  MEQKELLEELAQSAVASAT
Sbjct: 361 TYNYKDNRVTDHRLKMNFELTSFLEGDIENAVQACAAMEQKELLEELAQSAVASAT 416

[Close]
BLAST of Chy1G016550 vs. NCBI nr
Match: KAG6593177.1 (Peptide chain release factor APG3, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 727 bits (1877), Expect = 9.30e-263
Identity = 377/416 (90.62%), Postives = 392/416 (94.23%), Query Frame = 0 

Query: 1   MSIFNPTTAAPTIPSTFLLPPRHSGLFRRCSLELKRFAPPFRASTLICMAEPYLIVKLES 60
           M+ F+PTTA+ TIP  FL PPR+    RRCSLELKR APPFRASTL+CMAEPYLI KLES
Sbjct: 1   MNFFSPTTASRTIPGCFLFPPRYGDFSRRCSLELKRSAPPFRASTLVCMAEPYLITKLES 60

Query: 61  AEKTWKELSVKLADPDIVSNPTEYQKLAQSMSELDEVVSTFRKFKECEKQLEEAKALSKE 120
           AE TWKELSVKLADPDIVSNPTEYQKLAQSMSELDEVVSTFR+FKECEKQL+EAKAL+KE
Sbjct: 61  AENTWKELSVKLADPDIVSNPTEYQKLAQSMSELDEVVSTFRRFKECEKQLQEAKALAKE 120

Query: 121 SGNDEDMVEMIALEISSLSSELKDIEEKLKVMLLPSDPLDARNIMLEVRAGTGGDEAGIW 180
           SGNDEDM EMIALEISSLSS+LKDIEEKLKVMLLPSDPLDARNIMLEVRAGTGGDEAGIW
Sbjct: 121 SGNDEDMAEMIALEISSLSSQLKDIEEKLKVMLLPSDPLDARNIMLEVRAGTGGDEAGIW 180

Query: 181 AGDLVRMYQKYSERNSWKFSPISSSEAEKGGYKTYVLEIKGQCVYSKLKYESGVHRVQRV 240
           AGDLVRMYQKYSERNSWKFSPISSSEAEKGG+KTYVLEIKG  VYSKLKYESGVHRVQRV
Sbjct: 181 AGDLVRMYQKYSERNSWKFSPISSSEAEKGGFKTYVLEIKGNRVYSKLKYESGVHRVQRV 240

Query: 241 PQTETQGRVHTSTATVAIMPEADEVEVVIDPKDIELTTARSGGAGGQNVNKVETAIDLFH 300
           PQTETQGRVHTSTATVAIMPEADEVEVVIDPKDIELTT RSGGAGGQNVNKVETAIDLFH
Sbjct: 241 PQTETQGRVHTSTATVAIMPEADEVEVVIDPKDIELTTTRSGGAGGQNVNKVETAIDLFH 300

Query: 301 KPTGIRIFCTEERTQLQNKLRAFQLLRAKLYEIKVREQQEKIRNQRKLQVGTGARAEKIR 360
           KPTGIRIFCTEERTQLQNK RAFQLLRAKLYEIKVREQQE+IRNQRKLQVGTGARAEKIR
Sbjct: 301 KPTGIRIFCTEERTQLQNKHRAFQLLRAKLYEIKVREQQERIRNQRKLQVGTGARAEKIR 360

Query: 361 TYNYKDNRVTDHRLKTNFELTSFLEGDIEDAVQACVTMEQKELLEELAQSAVASAT 416
           TYNYKDNRVTDHRLK NFELTSF++GDIE+AVQACV MEQKEL+EEL QS  A  T
Sbjct: 361 TYNYKDNRVTDHRLKMNFELTSFIDGDIENAVQACVAMEQKELMEELVQSTGAPTT 416

[Close]
BLAST of Chy1G016550 vs. TAIR 10
Match: AT3G62910.1 (Peptide chain release factor 1 )

HSP 1 Score: 599.0 bits (1543), Expect = 3.0e-171
Identity = 304/374 (81.28%), Postives = 344/374 (91.98%), Query Frame = 0 

Query: 42  RASTLICMAEPYLIVKLESAEKTWKELSVKLADPDIVSNPTEYQKLAQSMSELDEVVSTF 101
           R   L+CMAEPYLI K+ES EKTWKELSVKLADPD+VSN +EYQKLAQSMSELDEVV+ F
Sbjct: 45  RTRRLVCMAEPYLIRKMESVEKTWKELSVKLADPDVVSNQSEYQKLAQSMSELDEVVTVF 104

Query: 102 RKFKECEKQLEEAKALSKESGNDEDMVEMIALEISSLSSELKDIEEKLKVMLLPSDPLDA 161
           R+FK+CEKQL E+K L+KE+G+DEDM EMI  EI+SL+ E++++E++LKV+LLPSDPLDA
Sbjct: 105 RRFKDCEKQLLESKVLAKEAGDDEDMAEMIGSEINSLTKEIEELEKQLKVLLLPSDPLDA 164

Query: 162 RNIMLEVRAGTGGDEAGIWAGDLVRMYQKYSERNSWKFSPISSSEAEKGGYKTYVLEIKG 221
           RNI+LEVRAGTGGDEA IW GDLVRMYQ+YSER+SWKFS +S SEAE GGYKT V+EIKG
Sbjct: 165 RNILLEVRAGTGGDEAAIWTGDLVRMYQRYSERSSWKFSMVSCSEAEHGGYKTCVMEIKG 224

Query: 222 QCVYSKLKYESGVHRVQRVPQTETQGRVHTSTATVAIMPEADEVEVVIDPKDIELTTARS 281
             VYSKLKYESGVHRVQRVPQTETQGRVHTSTATVAIMPEADEVEVVIDPKDIELT+ARS
Sbjct: 225 NRVYSKLKYESGVHRVQRVPQTETQGRVHTSTATVAIMPEADEVEVVIDPKDIELTSARS 284

Query: 282 GGAGGQNVNKVETAIDLFHKPTGIRIFCTEERTQLQNKLRAFQLLRAKLYEIKVREQQEK 341
           GGAGGQNVNKVETAIDLFHKP+GIRIFCTEERTQ++NK RAFQLLRAKLYEIKVREQQEK
Sbjct: 285 GGAGGQNVNKVETAIDLFHKPSGIRIFCTEERTQIRNKARAFQLLRAKLYEIKVREQQEK 344

Query: 342 IRNQRKLQVGTGARAEKIRTYNYKDNRVTDHRLKTNFELTSFLEGDIEDAVQACVTMEQK 401
           IRN+RK QVGTGAR+EKIRTYNYKD+RVTDHRLK NF LT+FL+G +EDAVQAC  +EQK
Sbjct: 345 IRNERKSQVGTGARSEKIRTYNYKDSRVTDHRLKMNFALTTFLDGALEDAVQACAALEQK 404

Query: 402 ELLEELAQSAVASA 416
           EL+EEL++S  AS+
Sbjct: 405 ELMEELSESVAASS 418

[Close]
BLAST of Chy1G016550 vs. TAIR 10
Match: AT2G47020.1 (Peptide chain release factor 1 )

HSP 1 Score: 236.1 bits (601), Expect = 5.1e-62
Identity = 125/323 (38.70%), Postives = 202/323 (62.54%), Query Frame = 0 

Query: 80  NPTEYQKLAQSMSELDEVVSTFRKFKECEKQLEEAKALSKESGNDEDMVEMIALEISSLS 139
           +P E+ +  + + +L + +      +  +K+++  K+L  ES +D+DM+++   E+    
Sbjct: 80  SPEEFSRANKELRKLRDSMLLINDLRAKQKEIDGLKSLVSESSDDKDMLDLAVGELDEAV 139

Query: 140 SELKDIEEKLKVMLLPSDPLDARNIMLEVRAGTGGDEAGIWAGDLVRMYQKYSERNSWKF 199
            E K ++  L   LLP D  D R+ +LEVRAGTGG+EA ++A D+ RMY++YS++  WKF
Sbjct: 140 EEEKRLQTLLLKSLLPKDEADERDCILEVRAGTGGEEASLFAMDIFRMYERYSQKKGWKF 199

Query: 200 SPISSSEAEKGGYKTYVLEIKGQCVYSKLKYESGVHRVQRVPQTETQGRVHTSTATVAIM 259
             +  +E++  GYK     I G  VY KLK+ESG+HRVQR+P TE  GR+HTS  +VAI+
Sbjct: 200 DIVDITESDMKGYKEASAAICGASVYGKLKFESGIHRVQRIPITEKSGRIHTSAISVAIL 259

Query: 260 PEADEVEVVIDPKDIELTTARSGGAGGQNVNKVETAIDLFHKPTGIRIFCTEERTQLQNK 319
           P+ADEV+V +  +D+ + T RSGG+GGQ+ N   +A+ + H PTG+ +   +ER+Q  N+
Sbjct: 260 PQADEVDVQLRNEDLRIDTYRSGGSGGQHANTTNSAVRIIHLPTGMMVSIQDERSQHMNR 319

Query: 320 LRAFQLLRAKLYEIKVREQQEKIRNQRKLQVGTGARAEKIRTYNYKDNRVTDHRLK-TNF 379
            +A ++L A+LYEI+    Q      R  Q+G+G R+ +IRTYN+   RVTDHR+  T+ 
Sbjct: 320 AKALKVLCARLYEIERLRIQSSRSKLRSDQIGSGDRSGRIRTYNFPQGRVTDHRVGITHH 379

Query: 380 ELTSFLEGDIEDAVQACVTMEQK 402
            +   ++G+  D     + + Q+
Sbjct: 380 AIEDMMQGENLDMFIDALLLRQE 402

[Close]
BLAST of Chy1G016550 vs. TAIR 10
Match: AT2G47020.2 (Peptide chain release factor 1 )

HSP 1 Score: 194.5 bits (493), Expect = 1.7e-49
Identity = 96/236 (40.68%), Postives = 153/236 (64.83%), Query Frame = 0 

Query: 80  NPTEYQKLAQSMSELDEVVSTFRKFKECEKQLEEAKALSKESGNDEDMVEMIALEISSLS 139
           +P E+ +  + + +L + +      +  +K+++  K+L  ES +D+DM+++   E+    
Sbjct: 15  SPEEFSRANKELRKLRDSMLLINDLRAKQKEIDGLKSLVSESSDDKDMLDLAVGELDEAV 74

Query: 140 SELKDIEEKLKVMLLPSDPLDARNIMLEVRAGTGGDEAGIWAGDLVRMYQKYSERNSWKF 199
            E K ++  L   LLP D  D R+ +LEVRAGTGG+EA ++A D+ RMY++YS++  WKF
Sbjct: 75  EEEKRLQTLLLKSLLPKDEADERDCILEVRAGTGGEEASLFAMDIFRMYERYSQKKGWKF 134

Query: 200 SPISSSEAEKGGYKTYVLEIKGQCVYSKLKYESGVHRVQRVPQTETQGRVHTSTATVAIM 259
             +  +E++  GYK     I G  VY KLK+ESG+HRVQR+P TE  GR+HTS  +VAI+
Sbjct: 135 DIVDITESDMKGYKEASAAICGASVYGKLKFESGIHRVQRIPITEKSGRIHTSAISVAIL 194

Query: 260 PEADEVEVVIDPKDIELTTARSGGAGGQNVNKVETAIDLFHKPTGIRIFCTEERTQ 316
           P+ADEV+V +  +D+ + T RSGG+GGQ+ N   +A+ + H PTG+ +   +ER+Q
Sbjct: 195 PQADEVDVQLRNEDLRIDTYRSGGSGGQHANTTNSAVRIIHLPTGMMVSIQDERSQ 250

[Close]
BLAST of Chy1G016550 vs. TAIR 10
Match: AT5G36170.1 (high chlorophyll fluorescent 109 )

HSP 1 Score: 155.2 bits (391), Expect = 1.1e-37
Identity = 113/349 (32.38%), Postives = 181/349 (51.86%), Query Frame = 0 

Query: 58  LESAEKTWKELSVKLADPDIVSNPTEYQKLAQSMSELDEVVSTFRKFKECEKQLEEAKAL 117
           L+  E+    L  K  D     + T+ Q+   S+++L + +    +FK   +  E    L
Sbjct: 104 LQQLEQEITNLESKATDTSFWDDRTKAQETLSSLNDLKDRMRLLSEFKTMVEDAETIVKL 163

Query: 118 SKE-SGNDEDMVEMIALEISSLSSELKDIEEKLKVMLLPSDPLDARNIMLEVRAGTGGDE 177
           ++E    D  ++E    E   +  EL    +K ++  L S P D    ++ + AG GG +
Sbjct: 164 TEEMDSTDVSLLE----EAMGIIKELNKSLDKFELTQLLSGPYDKEGAVVYITAGAGGTD 223

Query: 178 AGIWAGDLVRMYQKYSERNSWKFSPISSSEAEKGGYKTYVLEIKGQCVYSKLKYESGVHR 237
           A  WA  L+RMY ++ E+  +K   +  S  E+ G K+  LEI+G+  Y  +  E G HR
Sbjct: 224 AQDWADMLLRMYMRWGEKQRYKTKVVEMSNGEEAGIKSATLEIEGRYAYGYISGEKGTHR 283

Query: 238 VQRVPQTETQGRVHTSTATVAIMPEADE--VEVVIDPKDIELTTARSGGAGGQNVNKVET 297
           + R     ++G   TS + V +MP   E  V + I  +D++++  R+GG GGQNVNKVET
Sbjct: 284 IVRQSPFNSKGLRQTSFSGVEVMPLLPEEAVGIEIPEEDLDISFTRAGGKGGQNVNKVET 343

Query: 298 AIDLFHKPTGIRIFCTEERTQLQNKLRAFQLLRAKLYEIKVREQQEKIRNQRKLQVGTGA 357
           A+ + H PTG+ + CTEER+QL NK RA   L+AKL  I   ++  +I+  R    G   
Sbjct: 344 AVRITHIPTGVAVRCTEERSQLANKTRALIRLKAKLMVIAEEQRATEIKEIR----GDAV 403

Query: 358 RAE---KIRTYNY------KDNRVTDHRLKTNFELTSFLEGDIEDAVQA 395
           +AE   +IR Y +      KD R T H      ++TS ++GD++  ++A
Sbjct: 404 KAEWGQQIRNYVFHPYKLVKDVR-TGHETS---DITSVMDGDLDPFIKA 440

[Close]
BLAST of Chy1G016550 vs. TAIR 10
Match: AT5G36170.2 (high chlorophyll fluorescent 109 )

HSP 1 Score: 155.2 bits (391), Expect = 1.1e-37
Identity = 113/349 (32.38%), Postives = 181/349 (51.86%), Query Frame = 0 

Query: 58  LESAEKTWKELSVKLADPDIVSNPTEYQKLAQSMSELDEVVSTFRKFKECEKQLEEAKAL 117
           L+  E+    L  K  D     + T+ Q+   S+++L + +    +FK   +  E    L
Sbjct: 103 LQQLEQEITNLESKATDTSFWDDRTKAQETLSSLNDLKDRMRLLSEFKTMVEDAETIVKL 162

Query: 118 SKE-SGNDEDMVEMIALEISSLSSELKDIEEKLKVMLLPSDPLDARNIMLEVRAGTGGDE 177
           ++E    D  ++E    E   +  EL    +K ++  L S P D    ++ + AG GG +
Sbjct: 163 TEEMDSTDVSLLE----EAMGIIKELNKSLDKFELTQLLSGPYDKEGAVVYITAGAGGTD 222

Query: 178 AGIWAGDLVRMYQKYSERNSWKFSPISSSEAEKGGYKTYVLEIKGQCVYSKLKYESGVHR 237
           A  WA  L+RMY ++ E+  +K   +  S  E+ G K+  LEI+G+  Y  +  E G HR
Sbjct: 223 AQDWADMLLRMYMRWGEKQRYKTKVVEMSNGEEAGIKSATLEIEGRYAYGYISGEKGTHR 282

Query: 238 VQRVPQTETQGRVHTSTATVAIMPEADE--VEVVIDPKDIELTTARSGGAGGQNVNKVET 297
           + R     ++G   TS + V +MP   E  V + I  +D++++  R+GG GGQNVNKVET
Sbjct: 283 IVRQSPFNSKGLRQTSFSGVEVMPLLPEEAVGIEIPEEDLDISFTRAGGKGGQNVNKVET 342

Query: 298 AIDLFHKPTGIRIFCTEERTQLQNKLRAFQLLRAKLYEIKVREQQEKIRNQRKLQVGTGA 357
           A+ + H PTG+ + CTEER+QL NK RA   L+AKL  I   ++  +I+  R    G   
Sbjct: 343 AVRITHIPTGVAVRCTEERSQLANKTRALIRLKAKLMVIAEEQRATEIKEIR----GDAV 402

Query: 358 RAE---KIRTYNY------KDNRVTDHRLKTNFELTSFLEGDIEDAVQA 395
           +AE   +IR Y +      KD R T H      ++TS ++GD++  ++A
Sbjct: 403 KAEWGQQIRNYVFHPYKLVKDVR-TGHETS---DITSVMDGDLDPFIKA 439

The following BLAST results are available for this feature:
BLAST of Chy1G016550 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q8RX794.2e-17081.28Peptide chain release factor APG3, chloroplastic OS=Arabidopsis thaliana OX=3702... [more]
Q110D72.7e-12964.19Peptide chain release factor 1 OS=Trichodesmium erythraeum (strain IMS101) OX=20... [more]
B1XJH83.6e-12963.61Peptide chain release factor 1 OS=Synechococcus sp. (strain ATCC 27264 / PCC 700... [more]
Q8DMG96.1e-12964.44Peptide chain release factor 1 OS=Thermosynechococcus elongatus (strain BP-1) OX... [more]
B7KI178.0e-12964.64Peptide chain release factor 1 OS=Gloeothece citriformis (strain PCC 7424) OX=65... [more]
BLAST of Chy1G016550 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0K9447.8e-22097.36RF_PROK_I domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G394660 P... [more]
A0A5A7VLD66.6e-21996.15Peptide chain release factor APG3 OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A1S3BRZ76.6e-21996.15peptide chain release factor APG3, chloroplastic OS=Cucumis melo OX=3656 GN=LOC1... [more]
A0A6J1DC401.1e-20892.31peptide chain release factor APG3, chloroplastic OS=Momordica charantia OX=3673 ... [more]
A0A6J1KZW86.4e-20690.63peptide chain release factor APG3, chloroplastic OS=Cucurbita maxima OX=3661 GN=... [more]
BLAST of Chy1G016550 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_031745234.19.55e-28899.04peptide chain release factor APG3, chloroplastic [Cucumis sativus] >KGN45452.2 h... [more]
XP_008451621.11.14e-27996.15PREDICTED: peptide chain release factor APG3, chloroplastic [Cucumis melo] >KAA0... [more]
XP_038876731.12.35e-27294.47peptide chain release factor APG3, chloroplastic isoform X1 [Benincasa hispida][more]
XP_022151413.12.92e-26692.31peptide chain release factor APG3, chloroplastic [Momordica charantia][more]
KAG6593177.19.30e-26390.63Peptide chain release factor APG3, chloroplastic, partial [Cucurbita argyrosperm... [more]
BLAST of Chy1G016550 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G62910.13.0e-17181.28Peptide chain release factor 1 [more]
AT2G47020.15.1e-6238.70Peptide chain release factor 1 [more]
AT2G47020.21.7e-4940.68Peptide chain release factor 1 [more]
AT5G36170.11.1e-3732.38high chlorophyll fluorescent 109 [more]
AT5G36170.21.1e-3732.38high chlorophyll fluorescent 109 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 328..348
NoneNo IPR availableCOILSCoilCoilcoord: 128..148
NoneNo IPR availableGENE3D6.10.140.1950coord: 49..158
e-value: 5.1E-27
score: 96.3
NoneNo IPR availableGENE3D3.30.70.1660coord: 160..269
e-value: 1.5E-46
score: 160.2
NoneNo IPR availableGENE3D3.30.70.1660coord: 330..416
e-value: 7.9E-13
score: 50.4
NoneNo IPR availableGENE3D3.30.160.20coord: 270..329
e-value: 1.1E-23
score: 84.8
NoneNo IPR availablePANTHERPTHR43804LD18447Pcoord: 41..414
NoneNo IPR availablePANTHERPTHR43804:SF4PEPTIDE CHAIN RELEASE FACTOR APG3, CHLOROPLASTICcoord: 41..414
NoneNo IPR availableSUPERFAMILY75620Release factorcoord: 63..404
IPR005139Peptide chain release factorSMARTSM00937PCRF_a_2coord: 116..231
e-value: 7.9E-53
score: 191.5
IPR005139Peptide chain release factorPFAMPF03462PCRFcoord: 62..256
e-value: 1.2E-68
score: 230.7
IPR004373Peptide chain release factor 1TIGRFAMTIGR00019TIGR00019coord: 50..408
e-value: 1.5E-142
score: 472.7
IPR004373Peptide chain release factor 1HAMAPMF_00093Rel_fac_1coord: 54..408
score: 34.395844
IPR000352Peptide chain release factor class IPFAMPF00472RF-1coord: 263..373
e-value: 4.6E-35
score: 120.0
IPR000352Peptide chain release factor class IPROSITEPS00745RF_PROK_Icoord: 280..296

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy1G016550.1Chy1G016550.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009658 chloroplast organization
biological_process GO:0032544 plastid translation
biological_process GO:0010027 thylakoid membrane organization
biological_process GO:0006415 translational termination
cellular_component GO:0009507 chloroplast
molecular_function GO:0016149 translation release factor activity, codon specific
molecular_function GO:0003747 translation release factor activity