Chy1G016040 (gene) Cucumber (hystrix) v1

Overview
NameChy1G016040
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionMitochondrial substrate carrier family protein
LocationchrH01: 21979150 .. 21984327 (+)
RNA-Seq ExpressionChy1G016040
SyntenyChy1G016040
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTTAACAAATCTTCTTCTCCCCAAGAATCCTCCATTTCAGGTTCTCTCTCTATTCCTTCATTTATTATTGTTGTTATTATTATTATTAATCTTCCAATTCCATCTTTCTTCTTCTGTTTGCTTCAATTTCGATCAATTCTATTCAGGAGTTTTGCCGCGCAATGAGAGACGGAATTGGGCAGCTTCTCCCTCTGATGTTCTTAATCATTTTGGAACCAGCGGCCTCTCCGTCGCCATCGCCACCGCCATTACTCACCCTTTAGGTACTGTCTATCTCTTTCTCTCTCTCTCTCTCTCTCTCTATATATATATATATTTATATTTTCCTTTATAATCTTGAAAAATGCCTTTCCTGTTCTTGTTTTGTTGGTTTAGAAATTTAAAATTCAGGTGTCGTTGATGGGTATTTTGTGGTTCATTTGGATTTTAAGTGTATGTCATCCTTGTAAGTTGTTAGGTTGTAGTGGACTTAGAATTGAATCTGATGCAACCGTTTTGATTTCTTAGAGTTACGCTCATGGTGAGAATTTTGTGATCTGTGGAACTTTATAACGAATTTTTTCCTGGTAATTGTTGTTTGATGTAGAAAAAAATAGGTTAACTCAACTGGGATCAGTATGTGTTGAGGATCAAGATGTTCGTGGTTCAAATCTCCTATCTGTTTGTAATGAAAAAATATTTGATATTTGATAATAGTAATGCTTAGTACATGCTATGGTCGATATATGAATGACGAATGGAAAGCTGGAAGGAGTTAATAAGAGAATTTCGGAACTAGAACAAATTGGTTGTTTTTTGGCAAGCTGTTGTTGATGTAACCCTTTCTTATTCTGAACCACGTAGAAATGACATGAACGATTCTTAATTTTCAGAAGTGAGAGAGAGAGAGAGAGAAGAAATTGATCCTATAGCCAACAAACAGTGAGCTTAATTTGTCTCCAGCTTGAATGCAGATGTACTTAAAGTTAGGTTGCAAATGCAACTTGTGGGGCAGAGGGGGCCTCTGATTGGAATGGTATTCACGTTTTTCTTATAAGGGTTTGGTTTTTTTCCTTGAAGTTTCTTGTTCTATTGTACATTGATTGTCGAACATGCATAGGGACAAGTATTTGTGCAACTGTTGAATAATGAAGGGCCAAGGTCTTTGTATTTGGGGTTATCACCAGCTCTGACCAGATCAGTTCTCTATGGAGGTCTCCGTCTAGGTTTATATGAACCATCAAAGCATGCTTCTGATCTGCTTTTTGGGTCTACTAATATATTTGTTAAGATTGGAGCTGGAGCACTTGCTGGTGCAGTTGCAACTGCTCTAACCAATCCAATTGAAGTTCTCAAGGTGATATTTAGATTTCCACTCATTACTTAACTTTCCAATTATTGTTTGGTCTTAAAAGTTATTTGTTATTGCCAGGTGCGATTACAGATGAATCCAAACTCAACCAAAGGACCAATAAAAGAAATGTCCAGAATTGTTTCTGAGGAGGGACTAAAAGCTCTTTGGAAGGGTGTTGGTCCTGCTATGGCCAGAGCAGGTGCTTTGACAGCATCTCAGCTGGCGACATATGATGAATCAAAGCAGGTCTATTTCTAAGCATGTTCTTTTCATTTTAAACTTGCATGAAGTAATTTCCACAGTTTCTGATTTTTGAATCTGTATATATATGTTATAGCTCTGCAAGCTTATTTATTCTTATGTTTTTTTTTATCCTTAAAGTTTTTCCTATTTTGTATTTTGCAGCTTTTAGTTAAGTGGACACCCTTACAAGAAGGATTTTCTTTGCATCTCATGTATGAAACTCTGACCTATCTGTACAAATGTTTTGTTATCTATATTGTTTCCCCTTTCATTAATGTTTTGTTATCTATATTGTTTCCCCTTTCATTTTAGCCTTAAAACTCATTTATTTTATGATGCCATGCTTCCTATTTGAATATGCAGGCTTTATGTAATATATTATCACGTTAACAGAAAAAATTATTAAATGGTTAGCTGGCCAAGAATCGGCCCTTAAACAAGAATTTGGAGTTTTTAGGAAAAGTAATGATACTACACTCTAATCAGCTCGAACCAAAAACAGAAGGAAATTATATGCTTCTGTTGGTAGAAGCCCTGAGGGAGACCATAAAATGGATATGATCCCAAACCCCTTGATTCTTTTACTTATTAAAATTTAAAATTAAAATAGACATTCTATTTCCCCAAAAGATGTCTAAAACTGAATTCTTGTGGTCGGAAATTTGGTATATCTGTAAAAGTGGCAAGGAAGTTCCCTCTTGAAGCTTAGGGGGCGTTTGGGCTCCCAAAGCCCAACTTCAAGTGGGTGGAGTGGCTATGGCTTCAATTATAGTAGTTAGTGTTCCAACTATTAATACCTACAATTAACTATGGTGTTTACTATTTCCTACTTATCATCTCTCTCTTTTTGTTACAGTATTTACTATTTCATACTCGGACTAAAATAGTATGCACCCCAAACACAAACTTTTATAACTCGGACTAAAATAGTTTGCAATCCAAATGCAAATGATTATAACCTACGGGCTATTATAGCCCACATACTATAATAACCAACTTAGCATCCCAAAACACTCATTGTTGTCCTTTTATCCAACAATAACGGGTTGAAGAAGGATTTTCTAGCAACAAAAATAAAATAAAAGGCATTTCAGAAACTATTTAGTAGATTTTTCAATATTTACATACTAGCTTGTTTGGTTTTCAATTGTAACCGAAAGCTTTGTTTTGGTTTTTAGGTTGTGGTTTCAACCCAATTCTATCAATTGGCAAATGTTCTATTTTTCTTTTCATTTCTACACACAGATATTCATCATTTGAACTTTATTTATTTATTTTTTATTATTATTTTTGTTAGGAACCGAAGTACCGACTTTCGTTTGGAGAAATGAAAGATTGTACAAGGGCATAAAAAAATGAAAAAACAACCCAGCAAAAAAGGGAGACCTAAAGAAATTAGAAAATGGAACTCCAATACAACAAATTAAGATCATAGATACAAAAAGAGAGACCATTTGAACATTATTTACATAATATTTTTAAATTCAACTGTTTAAATCTCTCGTAGTTTACATCAATGACTAACCATTTGTTGCACCCTTGTGAAGCTCAAGTACAGTAGCTGGAGTGGTGAGTACTCTCATGACAACGCCTATTGACATGATTAAAACACGTCTTATGTTACAACGTGAGTCTAAAAGAGTTGGAAATTACAAGAACGGACTACATTGTGCATATCAGGTATATAACCTACCCACATTTCCTCTTTTCCTTTATCTTGCATCTAAATGTATTCTCTATATGCAAAATAGCATCGTACATATTGGATTTTCACACAGTGTGATTGCTTATCCTTCTCCTTTTAAGTCCATGCTTTTTTCCGCATTAATTAATGTTAGCTTCGATGTGGGAGGTGGAAAAGTTTTAGTGTCAAGCAAGCAAAAACTCCCCATTTTATTAATTCTTTTAAGGGGCATTTTTAAAAATAGCAAAATAAATTAAAAAAATTGCAAGTTATAGCAAAATTTTGGATTCTATCACTGATAGTCTTCTATCACTATATCATATCAATGACTCTCAGTGATAGAATTTGCTATATGTTGTAAATATTTTGTAAAATCTACTATTTTTAAAAAATTTCCTTCTTTTAATCGTAAATGTAACTATCTTGCAGCATGAAGGTTGTCTATTTCCTTAAAATTAATTATCTTAACCATGTATTTCTGTAAGAAGTTCGTCTTTAGATGACAGAAATTGTGAGATTGTATCCTTGTACAATCCACTTATCTTATCAAACTTTTGTTTTATTGAATATGACCAAAGTCTTGGTTGGACAAGGGAGAAGATTCATGATATATAAATGAAGACAATTATCTCTAATAGTATGGAGCTTTTTAGGTGAAACCAAAAGTAAGGCCACGAGGGTTTATGTCTAACGTGGGTGATATCATAATCAATACCATCTTGGAGATATGTGGAGGGTCATTGTCCCCTACATATTTCACCTAAGTCCATGGCATATTTAGAGTGTTACAACAATGATGGATAAAGATTTATATTATCTTAACTTTTCTACCATCTAACACCCTATCTGTTTACTTCAAAGCTCGGTTTCATTCAATTGGTTATTTTCATTTGTCTTTAGTGTCTTGCACACTTGTAGGCGTTAATCAACTATGGTTGAAGTTTCAACCTTGATTACACCTATATAGCTTGATCTTGCATAAAAAAGAATTGTAATATGTACGCAGTTGGAAAGTAAATTTACTTACATCATATATAATTCTTTTTAAATTTTTAATTATTTGCTCAACAATGGTGGCAACAAGATACCCATGCCAGAAAAGACATACCATTTCCTCCATCTCCTTTCCAACTTTGATTTGTATTCATTTGGTGTCATTGATTACTTGAATCTATATCTAACAATGGGGTGAGCAACTGATCTTTATAGGCTAATTTTTTTAATGGGGAACCTTCATTACCGGGAGTGTTTGATCCTCCTTGCCCTGCTTTCATTATTCTTAGTTATCACGTTCTTGCTAATTACTAAGATGGTCCATAATTTATTGTCCATGTCTCACAACATCAGTAGGCCTCAGAAAGCATGCTCCTTCTATCTATTTTGAGGGTCCTTTTTAAAGTGGTTGGGGATTCACTCGTGATATCCACTGTGACTCTCAGTTGTCTTAGGGAGCTGAGTTTTCTCTTCCATTTTCATATTATTTTTACAACTCATCCTCATTTCGCTTAGCAATAATGTGTAGATTGTCTCATATGGATTTGTGGAATTAATTTCAGGTTGTGCTAACTGAAGGGCCTTTGGCACTTTACAAGGGGTGAGTATGTTCTTTTCCAATCCCTCATTTTCAATAGCTCAAAAAAGTGTGTAAATGTATATATGCTTTTCTTTTTAGAAACAGTACATACATATAATAAAGTTCTTTAAGTACTTTTTTATAGCCTACATGACTGTTTCCAACTATGCAAAGAAACATGCATTTTTATGAGCTTTAAAAGTGTTTTGTGTAAGAACAGGTTTGCTGACTCAGAATATTTGCAGGGGCCTAGCCATTTTTGCAAGATTGGGTCCACAAACCACAATCACATTCATAGTCTGTGAGAAGTTACGCCAGCTTGCAGGATTAAATGCAATCTAG

mRNA sequence

ATGGTTAACAAATCTTCTTCTCCCCAAGAATCCTCCATTTCAGGAGTTTTGCCGCGCAATGAGAGACGGAATTGGGCAGCTTCTCCCTCTGATGTTCTTAATCATTTTGGAACCAGCGGCCTCTCCGTCGCCATCGCCACCGCCATTACTCACCCTTTAGATGTACTTAAAGTTAGGTTGCAAATGCAACTTGTGGGGCAGAGGGGGCCTCTGATTGGAATGGGACAAGTATTTGTGCAACTGTTGAATAATGAAGGGCCAAGGTCTTTGTATTTGGGGTTATCACCAGCTCTGACCAGATCAGTTCTCTATGGAGGTCTCCGTCTAGGTTTATATGAACCATCAAAGCATGCTTCTGATCTGCTTTTTGGGTCTACTAATATATTTGTTAAGATTGGAGCTGGAGCACTTGCTGGTGCAGTTGCAACTGCTCTAACCAATCCAATTGAAGTTCTCAAGGTGCGATTACAGATGAATCCAAACTCAACCAAAGGACCAATAAAAGAAATGTCCAGAATTGTTTCTGAGGAGGGACTAAAAGCTCTTTGGAAGGGTGTTGGTCCTGCTATGGCCAGAGCAGGTGCTTTGACAGCATCTCAGCTGGCGACATATGATGAATCAAAGCAGCTTTTAGTTAAGTGGACACCCTTACAAGAAGGATTTTCTTTGCATCTCATCTCAAGTACAGTAGCTGGAGTGGTGAGTACTCTCATGACAACGCCTATTGACATGATTAAAACACGTCTTATGTTACAACGTGAGTCTAAAAGAGTTGGAAATTACAAGAACGGACTACATTGTGCATATCAGGTTGTGCTAACTGAAGGGCCTTTGGCACTTTACAAGGGGGGCCTAGCCATTTTTGCAAGATTGGGTCCACAAACCACAATCACATTCATAGTCTGTGAGAAGTTACGCCAGCTTGCAGGATTAAATGCAATCTAG

Coding sequence (CDS)

ATGGTTAACAAATCTTCTTCTCCCCAAGAATCCTCCATTTCAGGAGTTTTGCCGCGCAATGAGAGACGGAATTGGGCAGCTTCTCCCTCTGATGTTCTTAATCATTTTGGAACCAGCGGCCTCTCCGTCGCCATCGCCACCGCCATTACTCACCCTTTAGATGTACTTAAAGTTAGGTTGCAAATGCAACTTGTGGGGCAGAGGGGGCCTCTGATTGGAATGGGACAAGTATTTGTGCAACTGTTGAATAATGAAGGGCCAAGGTCTTTGTATTTGGGGTTATCACCAGCTCTGACCAGATCAGTTCTCTATGGAGGTCTCCGTCTAGGTTTATATGAACCATCAAAGCATGCTTCTGATCTGCTTTTTGGGTCTACTAATATATTTGTTAAGATTGGAGCTGGAGCACTTGCTGGTGCAGTTGCAACTGCTCTAACCAATCCAATTGAAGTTCTCAAGGTGCGATTACAGATGAATCCAAACTCAACCAAAGGACCAATAAAAGAAATGTCCAGAATTGTTTCTGAGGAGGGACTAAAAGCTCTTTGGAAGGGTGTTGGTCCTGCTATGGCCAGAGCAGGTGCTTTGACAGCATCTCAGCTGGCGACATATGATGAATCAAAGCAGCTTTTAGTTAAGTGGACACCCTTACAAGAAGGATTTTCTTTGCATCTCATCTCAAGTACAGTAGCTGGAGTGGTGAGTACTCTCATGACAACGCCTATTGACATGATTAAAACACGTCTTATGTTACAACGTGAGTCTAAAAGAGTTGGAAATTACAAGAACGGACTACATTGTGCATATCAGGTTGTGCTAACTGAAGGGCCTTTGGCACTTTACAAGGGGGGCCTAGCCATTTTTGCAAGATTGGGTCCACAAACCACAATCACATTCATAGTCTGTGAGAAGTTACGCCAGCTTGCAGGATTAAATGCAATCTAG

Protein sequence

MVNKSSSPQESSISGVLPRNERRNWAASPSDVLNHFGTSGLSVAIATAITHPLDVLKVRLQMQLVGQRGPLIGMGQVFVQLLNNEGPRSLYLGLSPALTRSVLYGGLRLGLYEPSKHASDLLFGSTNIFVKIGAGALAGAVATALTNPIEVLKVRLQMNPNSTKGPIKEMSRIVSEEGLKALWKGVGPAMARAGALTASQLATYDESKQLLVKWTPLQEGFSLHLISSTVAGVVSTLMTTPIDMIKTRLMLQRESKRVGNYKNGLHCAYQVVLTEGPLALYKGGLAIFARLGPQTTITFIVCEKLRQLAGLNAI*
Homology
BLAST of Chy1G016040 vs. ExPASy Swiss-Prot
Match: B0G143 (Mitochondrial substrate carrier family protein ucpB OS=Dictyostelium discoideum OX=44689 GN=ucpB PE=3 SV=1)

HSP 1 Score: 189.9 bits (481), Expect = 4.4e-47
Identity = 105/280 (37.50%), Postives = 171/280 (61.07%), Query Frame = 0

Query: 40  GLSVAIATAITHPLDVLKVRLQMQLVGQRGPLIGMGQVFVQLLNNEGPRSLYLGLSPALT 99
           GLS   A  +++P+DVLK R Q+   G     +G+    ++++ NEG  ++Y GL+P+L 
Sbjct: 15  GLSCMGAAVVSNPVDVLKTRFQIHGEGIDSKSLGLVNGTIKIIKNEGISAMYKGLTPSLL 74

Query: 100 RSVLYGGLRLGLYEPSKHASDLLFGSTNIFVKIGAGALAGAVATALTNPIEVLKVRLQMN 159
           R   Y  LR+G Y+  K+      G TN+  K+ +GAL+GA+   +T+P +++KVR+Q +
Sbjct: 75  REATYSTLRMGGYDVIKNYFIDSNGKTNLLSKVTSGALSGALGACITSPTDLIKVRMQAS 134

Query: 160 PNSTK--GPIKEMSRIVSEEGLKALWKGVGPAMARAGALTASQLATYDESKQLLVKWTPL 219
               K          I+++EG+K LWKGVGP   RA  LTASQ+ +YD  K +++    +
Sbjct: 135 SKGVKYDSISSAFKEIIAKEGIKGLWKGVGPTTQRAALLTASQIPSYDHIKHMILDHGII 194

Query: 220 Q-EGFSLHLISSTVAGVVSTLMTTPIDMIKTRLMLQR-ESKRVG-NYKNGLHCAYQVVLT 279
           Q +G  +H++SS  AG+++++ T+P+D++KTR+M Q  +S  VG  YK+   C  +   +
Sbjct: 195 QVDGLQVHIVSSIFAGLIASITTSPVDLVKTRIMNQPFDSNGVGLIYKSSYDCFKKTFQS 254

Query: 280 EGPLALYKGGLAIFARLGPQTTITFIVCEKLRQLAGLNAI 315
           EG   LYKG L  + R+GP T +TFI+ E LR+++G+  I
Sbjct: 255 EGISGLYKGFLPNWFRIGPHTIVTFILYEYLRKVSGIKPI 294

BLAST of Chy1G016040 vs. ExPASy Swiss-Prot
Match: O81845 (Mitochondrial uncoupling protein 1 OS=Arabidopsis thaliana OX=3702 GN=PUMP1 PE=1 SV=1)

HSP 1 Score: 159.5 bits (402), Expect = 6.4e-38
Identity = 99/298 (33.22%), Postives = 154/298 (51.68%), Query Frame = 0

Query: 26  AASPSDVL--NHFGTSGLSVAIATAITHPLDVLKVRLQMQLVGQRGPLI-----GMGQVF 85
           AA  SD+     F  S  +  +    T PLD  KVRLQ+Q     G +      G+    
Sbjct: 3   AAGKSDLSLPKTFACSAFAACVGEVCTIPLDTAKVRLQLQKSALAGDVTLPKYRGLLGTV 62

Query: 86  VQLLNNEGPRSLYLGLSPALTRSVLYGGLRLGLYEPSK--HASDLLFGSTNIFVKIGAGA 145
             +   EG RSL+ G+ P L R  L+GGLR+G+YEP K  +      G   +  KI AG 
Sbjct: 63  GTIAREEGLRSLWKGVVPGLHRQCLFGGLRIGMYEPVKNLYVGKDFVGDVPLSKKILAGL 122

Query: 146 LAGAVATALTNPIEVLKVRLQMN-------PNSTKGPIKEMSRIVSEEGLKALWKGVGPA 205
             GA+   + NP +++KVRLQ         P    G +   S IV +EG++ALW G+GP 
Sbjct: 123 TTGALGIMVANPTDLVKVRLQAEGKLAAGAPRRYSGALNAYSTIVRQEGVRALWTGLGPN 182

Query: 206 MARAGALTASQLATYDESKQLLVKWTPLQEGFSLHLISSTVAGVVSTLMTTPIDMIKTRL 265
           +AR   + A++LA+YD+ K+ ++K     +    H++S   AG  +  + +P+D++K+R+
Sbjct: 183 VARNAIINAAELASYDQVKETILKIPGFTDNVVTHILSGLGAGFFAVCIGSPVDVVKSRM 242

Query: 266 MLQRESKRVGNYKNGLHCAYQVVLTEGPLALYKGGLAIFARLGPQTTITFIVCEKLRQ 308
           M        G YK  + C  + + ++GP+A YKG +  F RLG    I F+  E+ ++
Sbjct: 243 MGDS-----GAYKGTIDCFVKTLKSDGPMAFYKGFIPNFGRLGSWNVIMFLTLEQAKK 295

BLAST of Chy1G016040 vs. ExPASy Swiss-Prot
Match: O97562 (Mitochondrial uncoupling protein 2 OS=Sus scrofa OX=9823 GN=UCP2 PE=2 SV=1)

HSP 1 Score: 159.5 bits (402), Expect = 6.4e-38
Identity = 99/282 (35.11%), Postives = 147/282 (52.13%), Query Frame = 0

Query: 39  SGLSVAIATAITHPLDVLKVRLQMQLVGQRGPL--------IGMGQVFVQLLNNEGPRSL 98
           +G +  IA  IT PLD  KVRLQ+Q   +RGP+         G+    + ++ NEGPRSL
Sbjct: 20  AGTAACIADLITFPLDTAKVRLQIQ-GERRGPVQAAASAQYRGVLGTILTMVRNEGPRSL 79

Query: 99  YLGLSPALTRSVLYGGLRLGLYEPSKHASDLLFGSTNIFVKIGAGALAGAVATALTNPIE 158
           Y GL   L R + +  +R+GLY+  KH          I  ++ AG+  GA+A A+  P +
Sbjct: 80  YNGLVAGLQRQMSFASVRIGLYDSVKHFYTKGSEHAGIGSRLLAGSTTGALAVAVAQPTD 139

Query: 159 VLKVRLQMNPNSTKG-----PIKEMSRIVSEEGLKALWKGVGPAMARAGALTASQLATYD 218
           V+KVR Q    +  G      +     I  EEGL+ LWKG  P +AR   +  ++L TYD
Sbjct: 140 VVKVRFQAQARAGGGRRYRSTVDAYKTIAREEGLRGLWKGTSPNVARNAIVNCAELVTYD 199

Query: 219 ESKQLLVKWTPLQEGFSLHLISSTVAGVVSTLMTTPIDMIKTRLMLQRESKRVGNYKNGL 278
             K  L+K   + +    H  S+  AG  +T++ +P+D++KTR M    +   G Y +  
Sbjct: 200 LIKDTLLKADLMTDDLPCHFTSAFGAGFCTTVIASPVDVVKTRYM----NSAPGQYSSAG 259

Query: 279 HCAYQVVLTEGPLALYKGGLAIFARLGPQTTITFIVCEKLRQ 308
           HCA  ++  EGP A YKG    F RLG    + F+  E+L++
Sbjct: 260 HCALTMLQKEGPRAFYKGFTPSFLRLGSWNVVMFVTYEQLKR 296

BLAST of Chy1G016040 vs. ExPASy Swiss-Prot
Match: Q9SJY5 (Mitochondrial uncoupling protein 5 OS=Arabidopsis thaliana OX=3702 GN=PUMP5 PE=2 SV=1)

HSP 1 Score: 157.1 bits (396), Expect = 3.2e-37
Identity = 98/307 (31.92%), Postives = 168/307 (54.72%), Query Frame = 0

Query: 33  LNHFGTSGLSVAIATAITHPLDVLKVRLQMQLVGQRGPL--------------------- 92
           L  F   G++  +A   THPLD++KVR+Q+Q  G+  P+                     
Sbjct: 3   LKGFAEGGIASIVAGCSTHPLDLIKVRMQLQ--GESAPIQTNLRPALAFQTSTTVNAPPL 62

Query: 93  -IGMGQVFVQLLNNEGPRSLYLGLSPALTRSVLYGGLRLGLYEPSK-HASDLLFGSTNIF 152
            +G+  V  +L+  EG R+L+ G+S  + R  LY   R+GLY+  K   +D    +  + 
Sbjct: 63  RVGVIGVGSRLIREEGMRALFSGVSATVLRQTLYSTTRMGLYDIIKGEWTDPETKTMPLM 122

Query: 153 VKIGAGALAGAVATALTNPIEVLKVRLQ-------MNPNSTKGPIKEMSRIVSEEGLKAL 212
            KIGAGA+AGA+  A+ NP +V  VR+Q        +  + K  +  +++++  EG+ +L
Sbjct: 123 KKIGAGAIAGAIGAAVGNPADVAMVRMQADGRLPLTDRRNYKSVLDAITQMIRGEGVTSL 182

Query: 213 WKGVGPAMARAGALTASQLATYDESKQLLVKWTPLQEGFSLHLISSTVAGVVSTLMTTPI 272
           W+G    + RA  +T+SQLA+YD  K+ +++   L++G   H+ +S  AG V+++ + P+
Sbjct: 183 WRGSSLTINRAMLVTSSQLASYDSVKETILEKGLLKDGLGTHVSASFAAGFVASVASNPV 242

Query: 273 DMIKTRLMLQRESKRVG-NYKNGLHCAYQVVLTEGPLALYKGGLAIFARLGPQTTITFIV 309
           D+IKTR+M  +    V   YK  + CA + V  EG ++LYKG +   +R  P T + F+ 
Sbjct: 243 DVIKTRVMNMKVVAGVAPPYKGAVDCALKTVKAEGIMSLYKGFIPTVSRQAPFTVVLFVT 302

BLAST of Chy1G016040 vs. ExPASy Swiss-Prot
Match: Q54PY7 (Probable mitochondrial 2-oxoglutarate/malate carrier protein OS=Dictyostelium discoideum OX=44689 GN=ucpC PE=3 SV=1)

HSP 1 Score: 156.8 bits (395), Expect = 4.1e-37
Identity = 107/294 (36.39%), Postives = 152/294 (51.70%), Query Frame = 0

Query: 26  AASPSDVLNHFGTSGLSVAIATAITHPLDVLKVRLQMQLVGQR-GPLIGMGQVFVQLLNN 85
           A +P   L  F   GL+  +++A THP+D LKVR+Q+Q  G   GP  G  ++ V +   
Sbjct: 18  APTPQSQLKQFVIGGLAGMLSSAFTHPIDSLKVRMQLQGEGTGVGPKRGALKMLVHINQT 77

Query: 86  EGPRSLYLGLSPALTRSVLYGGLRLGLYEPSKHASDLLFGSTNIFVKIGAGALAGAVATA 145
           EG  +LY GLS +L R   Y   R GLY+  K              KI  G L+GA    
Sbjct: 78  EGFFTLYKGLSASLLRQATYTTTRFGLYDLIKDIVAKDDKPLPFTQKIMVGMLSGAGGAI 137

Query: 146 LTNPIEVLKVRLQMN-------PNSTKGPIKEMSRIVSEEGLKALWKGVGPAMARAGALT 205
           +  P ++  VR+Q +         + K     + RI  EEG+ +LWKG  P + RA  +T
Sbjct: 138 VGTPADLTMVRMQADGKLPFNLRRNYKNVFDGIFRISKEEGIISLWKGCSPNLIRAMFMT 197

Query: 206 ASQLATYDESKQLLVKWTPLQEGFSLHLISSTVAGVVSTLMTTPIDMIKTRLMLQRESKR 265
           A Q+++YD++KQL++      +    HLI+ST A  V+ + T+P+D+IKTR+M     K 
Sbjct: 198 AGQVSSYDQTKQLMLASGYFHDDIKTHLIASTTAAFVAAVATSPLDVIKTRIM--NSPKT 257

Query: 266 VG---NYKNGLHCAYQVVLTEGPLALYKGGLAIFARLGPQTTITFIVCEKLRQL 309
           V     YK    C  + +  EG  A YKG    F RLGPQT +TFI  E+L  L
Sbjct: 258 VTGELQYKGTFDCLSKTLRAEGFKAFYKGFNPYFMRLGPQTILTFIFVEQLNIL 309

BLAST of Chy1G016040 vs. ExPASy TrEMBL
Match: A0A1S3BSK0 (mitochondrial substrate carrier family protein ucpB isoform X1 OS=Cucumis melo OX=3656 GN=LOC103492725 PE=3 SV=1)

HSP 1 Score: 591.3 bits (1523), Expect = 2.4e-165
Identity = 303/314 (96.50%), Postives = 310/314 (98.73%), Query Frame = 0

Query: 1   MVNKSSSPQESSISGVLPRNERRNWAASPSDVLNHFGTSGLSVAIATAITHPLDVLKVRL 60
           MVNKSSSPQESSISGVLPRNERRNWAASPSDVL+HFGTSGLSVAIATA+THPLDVLKVRL
Sbjct: 1   MVNKSSSPQESSISGVLPRNERRNWAASPSDVLHHFGTSGLSVAIATAVTHPLDVLKVRL 60

Query: 61  QMQLVGQRGPLIGMGQVFVQLLNNEGPRSLYLGLSPALTRSVLYGGLRLGLYEPSKHASD 120
           QMQLVGQRGPLIGMGQVFVQLLNNEGPRSLYLGLSPALTRSVLYGGLRLGLYEPSKHASD
Sbjct: 61  QMQLVGQRGPLIGMGQVFVQLLNNEGPRSLYLGLSPALTRSVLYGGLRLGLYEPSKHASD 120

Query: 121 LLFGSTNIFVKIGAGALAGAVATALTNPIEVLKVRLQMNPNSTKGPIKEMSRIVSEEGLK 180
           LLFGSTNIFVKIGAGA+AGAVATALTNP+EVLKVRLQMNPNST G +KEMSRIVSEEGLK
Sbjct: 121 LLFGSTNIFVKIGAGAIAGAVATALTNPVEVLKVRLQMNPNSTNGSMKEMSRIVSEEGLK 180

Query: 181 ALWKGVGPAMARAGALTASQLATYDESKQLLVKWTPLQEGFSLHLISSTVAGVVSTLMTT 240
           ALWKGVGPAMARAGALTASQLATYDESK++LVKWTPLQEGF LHLISSTVAGVVSTLMTT
Sbjct: 181 ALWKGVGPAMARAGALTASQLATYDESKRVLVKWTPLQEGFPLHLISSTVAGVVSTLMTT 240

Query: 241 PIDMIKTRLMLQRESKRVGNYKNGLHCAYQVVLTEGPLALYKGGLAIFARLGPQTTITFI 300
           PIDMIKTRLMLQRESKR GNYKNGLHCAYQVVLTEGPLALYKGGLAIFARLGPQTTITFI
Sbjct: 241 PIDMIKTRLMLQRESKRAGNYKNGLHCAYQVVLTEGPLALYKGGLAIFARLGPQTTITFI 300

Query: 301 VCEKLRQLAGLNAI 315
           VCEKLRQLAGLNAI
Sbjct: 301 VCEKLRQLAGLNAI 314

BLAST of Chy1G016040 vs. ExPASy TrEMBL
Match: A0A6J1KWR8 (mitochondrial substrate carrier family protein ucpB isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111497854 PE=3 SV=1)

HSP 1 Score: 556.6 bits (1433), Expect = 6.6e-155
Identity = 283/314 (90.13%), Postives = 305/314 (97.13%), Query Frame = 0

Query: 1   MVNKSSSPQESSISGVLPRNERRNWAASPSDVLNHFGTSGLSVAIATAITHPLDVLKVRL 60
           MV++SSS QES+ISGVL RNE+RNWA SPSDV  HFGTSG SVA+ATA+THPLDVLKVRL
Sbjct: 1   MVDESSS-QESAISGVLARNEKRNWAVSPSDVGYHFGTSGFSVAVATAVTHPLDVLKVRL 60

Query: 61  QMQLVGQRGPLIGMGQVFVQLLNNEGPRSLYLGLSPALTRSVLYGGLRLGLYEPSKHASD 120
           QMQLVGQRGPLIGMGQ+FVQLLNNEGPRSLYLGLSPALTRSVLYGGLRLGLYEPSK+ASD
Sbjct: 61  QMQLVGQRGPLIGMGQLFVQLLNNEGPRSLYLGLSPALTRSVLYGGLRLGLYEPSKYASD 120

Query: 121 LLFGSTNIFVKIGAGALAGAVATALTNPIEVLKVRLQMNPNSTKGPIKEMSRIVSEEGLK 180
           L+FGSTNIFVKIG+GA++GAVATALTNP+EVLKVRLQMNPNST+GPIKEMS+IVSEEGLK
Sbjct: 121 LVFGSTNIFVKIGSGAISGAVATALTNPVEVLKVRLQMNPNSTRGPIKEMSKIVSEEGLK 180

Query: 181 ALWKGVGPAMARAGALTASQLATYDESKQLLVKWTPLQEGFSLHLISSTVAGVVSTLMTT 240
           ALWKGVGPAMARAGALTASQLATYDE+KQ+LVKWTPL+EGF LHLISSTVAGVVSTLMTT
Sbjct: 181 ALWKGVGPAMARAGALTASQLATYDEAKQVLVKWTPLEEGFPLHLISSTVAGVVSTLMTT 240

Query: 241 PIDMIKTRLMLQRESKRVGNYKNGLHCAYQVVLTEGPLALYKGGLAIFARLGPQTTITFI 300
           PIDM+KTRLMLQRESKR G+YK+GLHCAYQV+ TEGPLALYKGGLAIFARLGPQTTITFI
Sbjct: 241 PIDMVKTRLMLQRESKRAGSYKSGLHCAYQVIRTEGPLALYKGGLAIFARLGPQTTITFI 300

Query: 301 VCEKLRQLAGLNAI 315
           VCEKLRQLAGLNAI
Sbjct: 301 VCEKLRQLAGLNAI 313

BLAST of Chy1G016040 vs. ExPASy TrEMBL
Match: A0A6J1H690 (mitochondrial substrate carrier family protein ucpB isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111460838 PE=3 SV=1)

HSP 1 Score: 556.6 bits (1433), Expect = 6.6e-155
Identity = 283/314 (90.13%), Postives = 305/314 (97.13%), Query Frame = 0

Query: 1   MVNKSSSPQESSISGVLPRNERRNWAASPSDVLNHFGTSGLSVAIATAITHPLDVLKVRL 60
           MV++SSS QES+ISGVL RNE+RNWA SPSDV  HFGTSG SVAIATA+THPLDVLKVRL
Sbjct: 1   MVDESSS-QESAISGVLARNEKRNWAVSPSDVGYHFGTSGFSVAIATAVTHPLDVLKVRL 60

Query: 61  QMQLVGQRGPLIGMGQVFVQLLNNEGPRSLYLGLSPALTRSVLYGGLRLGLYEPSKHASD 120
           QMQLVGQRGPLIGMGQ+FVQLLNNEGPRSLYLGLSPALTRSVLYGGLRLGLYEPSK+ASD
Sbjct: 61  QMQLVGQRGPLIGMGQLFVQLLNNEGPRSLYLGLSPALTRSVLYGGLRLGLYEPSKYASD 120

Query: 121 LLFGSTNIFVKIGAGALAGAVATALTNPIEVLKVRLQMNPNSTKGPIKEMSRIVSEEGLK 180
           L+FGSTNIFVKIG+GA++GAVATALTNP+EVLKVRLQMNPNST+GPIKEMS+I+SEEGLK
Sbjct: 121 LVFGSTNIFVKIGSGAISGAVATALTNPVEVLKVRLQMNPNSTRGPIKEMSKIISEEGLK 180

Query: 181 ALWKGVGPAMARAGALTASQLATYDESKQLLVKWTPLQEGFSLHLISSTVAGVVSTLMTT 240
           ALWKGVGPAMARAGALTASQLATYDE+KQ+LVKWTPL+EGF LHLISSTVAGVVSTLMTT
Sbjct: 181 ALWKGVGPAMARAGALTASQLATYDEAKQVLVKWTPLEEGFPLHLISSTVAGVVSTLMTT 240

Query: 241 PIDMIKTRLMLQRESKRVGNYKNGLHCAYQVVLTEGPLALYKGGLAIFARLGPQTTITFI 300
           PIDM+KTRLMLQRESKR G+YK+GLHCAYQV+ TEGPLALYKGGLAIFARLGPQTTITFI
Sbjct: 241 PIDMVKTRLMLQRESKRAGSYKSGLHCAYQVIRTEGPLALYKGGLAIFARLGPQTTITFI 300

Query: 301 VCEKLRQLAGLNAI 315
           VCEKLRQLAGLNAI
Sbjct: 301 VCEKLRQLAGLNAI 313

BLAST of Chy1G016040 vs. ExPASy TrEMBL
Match: A0A6J1DA08 (mitochondrial substrate carrier family protein ucpB isoform X1 OS=Momordica charantia OX=3673 GN=LOC111018417 PE=3 SV=1)

HSP 1 Score: 552.7 bits (1423), Expect = 9.6e-154
Identity = 284/314 (90.45%), Postives = 300/314 (95.54%), Query Frame = 0

Query: 1   MVNKSSSPQESSISGVLPRNERRNWAASPSDVLNHFGTSGLSVAIATAITHPLDVLKVRL 60
           MVN+SS   ESS SGVL RNE+RNWA SPSDV+ HFGTSGLSVA+ATA+THPLDVLKVRL
Sbjct: 1   MVNESSY-HESSNSGVLVRNEKRNWAVSPSDVVYHFGTSGLSVAVATAVTHPLDVLKVRL 60

Query: 61  QMQLVGQRGPLIGMGQVFVQLLNNEGPRSLYLGLSPALTRSVLYGGLRLGLYEPSKHASD 120
           QMQLVGQRGPL GMG++FVQLLNNEGPRSLYLGLSPALTRSVLYGGLRLGLYEPSK+A D
Sbjct: 61  QMQLVGQRGPLTGMGRLFVQLLNNEGPRSLYLGLSPALTRSVLYGGLRLGLYEPSKYAYD 120

Query: 121 LLFGSTNIFVKIGAGALAGAVATALTNPIEVLKVRLQMNPNSTKGPIKEMSRIVSEEGLK 180
           LLFGSTNIFVKIG+GA++G VATALTNP+EVLKVRLQMNPNST GPIKEMSRIV+EEGLK
Sbjct: 121 LLFGSTNIFVKIGSGAISGXVATALTNPVEVLKVRLQMNPNSTGGPIKEMSRIVTEEGLK 180

Query: 181 ALWKGVGPAMARAGALTASQLATYDESKQLLVKWTPLQEGFSLHLISSTVAGVVSTLMTT 240
           ALWKGVGPAMARAGALTASQLATYDESKQ+LVKWTPLQEGF LHLISS VAGVVSTLMTT
Sbjct: 181 ALWKGVGPAMARAGALTASQLATYDESKQVLVKWTPLQEGFPLHLISSAVAGVVSTLMTT 240

Query: 241 PIDMIKTRLMLQRESKRVGNYKNGLHCAYQVVLTEGPLALYKGGLAIFARLGPQTTITFI 300
           PIDMIKTRLMLQRESK+ GNYKNGLHCAYQV+LTEGPLALYKGGLAIFARLGPQTTITFI
Sbjct: 241 PIDMIKTRLMLQRESKKAGNYKNGLHCAYQVMLTEGPLALYKGGLAIFARLGPQTTITFI 300

Query: 301 VCEKLRQLAGLNAI 315
           VCEKLRQLAGLNAI
Sbjct: 301 VCEKLRQLAGLNAI 313

BLAST of Chy1G016040 vs. ExPASy TrEMBL
Match: A0A0A0K8Y5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G390160 PE=3 SV=1)

HSP 1 Score: 501.9 bits (1291), Expect = 1.9e-138
Identity = 256/261 (98.08%), Postives = 261/261 (100.00%), Query Frame = 0

Query: 54  DVLKVRLQMQLVGQRGPLIGMGQVFVQLLNNEGPRSLYLGLSPALTRSVLYGGLRLGLYE 113
           DVLKVRLQMQLVGQRGPLIGMGQVFVQLLNNEGP+SLYLGLSPALTRSVLYGGLRLGLYE
Sbjct: 40  DVLKVRLQMQLVGQRGPLIGMGQVFVQLLNNEGPKSLYLGLSPALTRSVLYGGLRLGLYE 99

Query: 114 PSKHASDLLFGSTNIFVKIGAGALAGAVATALTNPIEVLKVRLQMNPNSTKGPIKEMSRI 173
           PSKHASDLLFGSTNIFVKIGAGA+AGAVATALTNP+EVLKVRLQMNPNSTKGP+KEMSRI
Sbjct: 100 PSKHASDLLFGSTNIFVKIGAGAIAGAVATALTNPVEVLKVRLQMNPNSTKGPMKEMSRI 159

Query: 174 VSEEGLKALWKGVGPAMARAGALTASQLATYDESKQLLVKWTPLQEGFSLHLISSTVAGV 233
           VSEEGLKALWKGVGPAMARAGALTASQLATYDESKQLLVKWTPLQEGFSLHLISSTVAGV
Sbjct: 160 VSEEGLKALWKGVGPAMARAGALTASQLATYDESKQLLVKWTPLQEGFSLHLISSTVAGV 219

Query: 234 VSTLMTTPIDMIKTRLMLQRESKRVGNYKNGLHCAYQVVLTEGPLALYKGGLAIFARLGP 293
           VSTLMTTPIDMIKTRLMLQRESKRVGNYKNGLHCAYQ+VLTEGPLALYKGGLAIFARLGP
Sbjct: 220 VSTLMTTPIDMIKTRLMLQRESKRVGNYKNGLHCAYQIVLTEGPLALYKGGLAIFARLGP 279

Query: 294 QTTITFIVCEKLRQLAGLNAI 315
           QTTITFIVCEKLRQLAGLNAI
Sbjct: 280 QTTITFIVCEKLRQLAGLNAI 300

BLAST of Chy1G016040 vs. NCBI nr
Match: XP_004148885.2 (mitochondrial substrate carrier family protein ucpB isoform X1 [Cucumis sativus] >KAE8646319.1 hypothetical protein Csa_015750 [Cucumis sativus])

HSP 1 Score: 605 bits (1560), Expect = 6.98e-218
Identity = 308/314 (98.09%), Postives = 314/314 (100.00%), Query Frame = 0

Query: 1   MVNKSSSPQESSISGVLPRNERRNWAASPSDVLNHFGTSGLSVAIATAITHPLDVLKVRL 60
           MVNKSSSPQESSISGVLPRNERRNWAASPSDVLNHFGTSGLSVAIATA+THPLDVLKVRL
Sbjct: 1   MVNKSSSPQESSISGVLPRNERRNWAASPSDVLNHFGTSGLSVAIATAVTHPLDVLKVRL 60

Query: 61  QMQLVGQRGPLIGMGQVFVQLLNNEGPRSLYLGLSPALTRSVLYGGLRLGLYEPSKHASD 120
           QMQLVGQRGPLIGMGQVFVQLLNNEGP+SLYLGLSPALTRSVLYGGLRLGLYEPSKHASD
Sbjct: 61  QMQLVGQRGPLIGMGQVFVQLLNNEGPKSLYLGLSPALTRSVLYGGLRLGLYEPSKHASD 120

Query: 121 LLFGSTNIFVKIGAGALAGAVATALTNPIEVLKVRLQMNPNSTKGPIKEMSRIVSEEGLK 180
           LLFGSTNIFVKIGAGA+AGAVATALTNP+EVLKVRLQMNPNSTKGP+KEMSRIVSEEGLK
Sbjct: 121 LLFGSTNIFVKIGAGAIAGAVATALTNPVEVLKVRLQMNPNSTKGPMKEMSRIVSEEGLK 180

Query: 181 ALWKGVGPAMARAGALTASQLATYDESKQLLVKWTPLQEGFSLHLISSTVAGVVSTLMTT 240
           ALWKGVGPAMARAGALTASQLATYDESKQLLVKWTPLQEGFSLHLISSTVAGVVSTLMTT
Sbjct: 181 ALWKGVGPAMARAGALTASQLATYDESKQLLVKWTPLQEGFSLHLISSTVAGVVSTLMTT 240

Query: 241 PIDMIKTRLMLQRESKRVGNYKNGLHCAYQVVLTEGPLALYKGGLAIFARLGPQTTITFI 300
           PIDMIKTRLMLQRESKRVGNYKNGLHCAYQ+VLTEGPLALYKGGLAIFARLGPQTTITFI
Sbjct: 241 PIDMIKTRLMLQRESKRVGNYKNGLHCAYQIVLTEGPLALYKGGLAIFARLGPQTTITFI 300

Query: 301 VCEKLRQLAGLNAI 314
           VCEKLRQLAGLNAI
Sbjct: 301 VCEKLRQLAGLNAI 314

BLAST of Chy1G016040 vs. NCBI nr
Match: XP_008451426.1 (PREDICTED: mitochondrial substrate carrier family protein ucpB isoform X1 [Cucumis melo])

HSP 1 Score: 593 bits (1530), Expect = 2.61e-213
Identity = 303/314 (96.50%), Postives = 310/314 (98.73%), Query Frame = 0

Query: 1   MVNKSSSPQESSISGVLPRNERRNWAASPSDVLNHFGTSGLSVAIATAITHPLDVLKVRL 60
           MVNKSSSPQESSISGVLPRNERRNWAASPSDVL+HFGTSGLSVAIATA+THPLDVLKVRL
Sbjct: 1   MVNKSSSPQESSISGVLPRNERRNWAASPSDVLHHFGTSGLSVAIATAVTHPLDVLKVRL 60

Query: 61  QMQLVGQRGPLIGMGQVFVQLLNNEGPRSLYLGLSPALTRSVLYGGLRLGLYEPSKHASD 120
           QMQLVGQRGPLIGMGQVFVQLLNNEGPRSLYLGLSPALTRSVLYGGLRLGLYEPSKHASD
Sbjct: 61  QMQLVGQRGPLIGMGQVFVQLLNNEGPRSLYLGLSPALTRSVLYGGLRLGLYEPSKHASD 120

Query: 121 LLFGSTNIFVKIGAGALAGAVATALTNPIEVLKVRLQMNPNSTKGPIKEMSRIVSEEGLK 180
           LLFGSTNIFVKIGAGA+AGAVATALTNP+EVLKVRLQMNPNST G +KEMSRIVSEEGLK
Sbjct: 121 LLFGSTNIFVKIGAGAIAGAVATALTNPVEVLKVRLQMNPNSTNGSMKEMSRIVSEEGLK 180

Query: 181 ALWKGVGPAMARAGALTASQLATYDESKQLLVKWTPLQEGFSLHLISSTVAGVVSTLMTT 240
           ALWKGVGPAMARAGALTASQLATYDESK++LVKWTPLQEGF LHLISSTVAGVVSTLMTT
Sbjct: 181 ALWKGVGPAMARAGALTASQLATYDESKRVLVKWTPLQEGFPLHLISSTVAGVVSTLMTT 240

Query: 241 PIDMIKTRLMLQRESKRVGNYKNGLHCAYQVVLTEGPLALYKGGLAIFARLGPQTTITFI 300
           PIDMIKTRLMLQRESKR GNYKNGLHCAYQVVLTEGPLALYKGGLAIFARLGPQTTITFI
Sbjct: 241 PIDMIKTRLMLQRESKRAGNYKNGLHCAYQVVLTEGPLALYKGGLAIFARLGPQTTITFI 300

Query: 301 VCEKLRQLAGLNAI 314
           VCEKLRQLAGLNAI
Sbjct: 301 VCEKLRQLAGLNAI 314

BLAST of Chy1G016040 vs. NCBI nr
Match: XP_031744482.1 (mitochondrial substrate carrier family protein ucpB isoform X2 [Cucumis sativus])

HSP 1 Score: 592 bits (1527), Expect = 6.44e-213
Identity = 304/314 (96.82%), Postives = 310/314 (98.73%), Query Frame = 0

Query: 1   MVNKSSSPQESSISGVLPRNERRNWAASPSDVLNHFGTSGLSVAIATAITHPLDVLKVRL 60
           MVNKSSSPQESSISGVLPRNERRNWAASPSDVLNHFGTSGLSVAIATA+THPLDVLKVRL
Sbjct: 1   MVNKSSSPQESSISGVLPRNERRNWAASPSDVLNHFGTSGLSVAIATAVTHPLDVLKVRL 60

Query: 61  QMQLVGQRGPLIGMGQVFVQLLNNEGPRSLYLGLSPALTRSVLYGGLRLGLYEPSKHASD 120
           QMQLVGQRGPLIGMGQVFVQLLNNEGP+SLYLGLSPALTRSVLYGGLRLGLYEPSKHASD
Sbjct: 61  QMQLVGQRGPLIGMGQVFVQLLNNEGPKSLYLGLSPALTRSVLYGGLRLGLYEPSKHASD 120

Query: 121 LLFGSTNIFVKIGAGALAGAVATALTNPIEVLKVRLQMNPNSTKGPIKEMSRIVSEEGLK 180
           LLFGSTNIFVKIGAGA+AGAVATALTNP+EVLK    MNPNSTKGP+KEMSRIVSEEGLK
Sbjct: 121 LLFGSTNIFVKIGAGAIAGAVATALTNPVEVLK----MNPNSTKGPMKEMSRIVSEEGLK 180

Query: 181 ALWKGVGPAMARAGALTASQLATYDESKQLLVKWTPLQEGFSLHLISSTVAGVVSTLMTT 240
           ALWKGVGPAMARAGALTASQLATYDESKQLLVKWTPLQEGFSLHLISSTVAGVVSTLMTT
Sbjct: 181 ALWKGVGPAMARAGALTASQLATYDESKQLLVKWTPLQEGFSLHLISSTVAGVVSTLMTT 240

Query: 241 PIDMIKTRLMLQRESKRVGNYKNGLHCAYQVVLTEGPLALYKGGLAIFARLGPQTTITFI 300
           PIDMIKTRLMLQRESKRVGNYKNGLHCAYQ+VLTEGPLALYKGGLAIFARLGPQTTITFI
Sbjct: 241 PIDMIKTRLMLQRESKRVGNYKNGLHCAYQIVLTEGPLALYKGGLAIFARLGPQTTITFI 300

Query: 301 VCEKLRQLAGLNAI 314
           VCEKLRQLAGLNAI
Sbjct: 301 VCEKLRQLAGLNAI 310

BLAST of Chy1G016040 vs. NCBI nr
Match: XP_038896316.1 (mitochondrial substrate carrier family protein ucpB isoform X1 [Benincasa hispida] >XP_038896317.1 mitochondrial substrate carrier family protein ucpB isoform X1 [Benincasa hispida] >XP_038896318.1 mitochondrial substrate carrier family protein ucpB isoform X1 [Benincasa hispida])

HSP 1 Score: 572 bits (1475), Expect = 6.06e-205
Identity = 295/314 (93.95%), Postives = 307/314 (97.77%), Query Frame = 0

Query: 1   MVNKSSSPQESSISGVLPRNERRNWAASPSDVLNHFGTSGLSVAIATAITHPLDVLKVRL 60
           MVN SSS QESSISGVL RNERR WAASPSDVL HFGTSGLSVA+ATA+THPLDVLKVRL
Sbjct: 1   MVNGSSS-QESSISGVLARNERRIWAASPSDVLYHFGTSGLSVAVATAVTHPLDVLKVRL 60

Query: 61  QMQLVGQRGPLIGMGQVFVQLLNNEGPRSLYLGLSPALTRSVLYGGLRLGLYEPSKHASD 120
           QMQLVG+RGPLIGMGQVF+QLLNNEGPRSLYLGLSPALTRSVLYGGLRLGLYEPSK+ASD
Sbjct: 61  QMQLVGRRGPLIGMGQVFLQLLNNEGPRSLYLGLSPALTRSVLYGGLRLGLYEPSKYASD 120

Query: 121 LLFGSTNIFVKIGAGALAGAVATALTNPIEVLKVRLQMNPNSTKGPIKEMSRIVSEEGLK 180
           LLFGSTNIFVKIGAGA++GAVATALTNP+EVLKVRLQMNPNSTKGPIKEMSRIVSEEGLK
Sbjct: 121 LLFGSTNIFVKIGAGAISGAVATALTNPVEVLKVRLQMNPNSTKGPIKEMSRIVSEEGLK 180

Query: 181 ALWKGVGPAMARAGALTASQLATYDESKQLLVKWTPLQEGFSLHLISSTVAGVVSTLMTT 240
           ALWKGVGPAMARAGALTASQLATYDESK++LVKWTPLQEGF LHLISSTVAGVVSTLMTT
Sbjct: 181 ALWKGVGPAMARAGALTASQLATYDESKRVLVKWTPLQEGFPLHLISSTVAGVVSTLMTT 240

Query: 241 PIDMIKTRLMLQRESKRVGNYKNGLHCAYQVVLTEGPLALYKGGLAIFARLGPQTTITFI 300
           PIDMIKTRLMLQRESKR G+YKNGLHCAYQV+LTEGPLALYKGGLAIFARLGPQTTITFI
Sbjct: 241 PIDMIKTRLMLQRESKRAGSYKNGLHCAYQVMLTEGPLALYKGGLAIFARLGPQTTITFI 300

Query: 301 VCEKLRQLAGLNAI 314
           VCEKLRQLAGLNAI
Sbjct: 301 VCEKLRQLAGLNAI 313

BLAST of Chy1G016040 vs. NCBI nr
Match: XP_023004604.1 (mitochondrial substrate carrier family protein ucpB isoform X1 [Cucurbita maxima])

HSP 1 Score: 559 bits (1440), Expect = 1.31e-199
Identity = 283/314 (90.13%), Postives = 305/314 (97.13%), Query Frame = 0

Query: 1   MVNKSSSPQESSISGVLPRNERRNWAASPSDVLNHFGTSGLSVAIATAITHPLDVLKVRL 60
           MV++SSS QES+ISGVL RNE+RNWA SPSDV  HFGTSG SVA+ATA+THPLDVLKVRL
Sbjct: 1   MVDESSS-QESAISGVLARNEKRNWAVSPSDVGYHFGTSGFSVAVATAVTHPLDVLKVRL 60

Query: 61  QMQLVGQRGPLIGMGQVFVQLLNNEGPRSLYLGLSPALTRSVLYGGLRLGLYEPSKHASD 120
           QMQLVGQRGPLIGMGQ+FVQLLNNEGPRSLYLGLSPALTRSVLYGGLRLGLYEPSK+ASD
Sbjct: 61  QMQLVGQRGPLIGMGQLFVQLLNNEGPRSLYLGLSPALTRSVLYGGLRLGLYEPSKYASD 120

Query: 121 LLFGSTNIFVKIGAGALAGAVATALTNPIEVLKVRLQMNPNSTKGPIKEMSRIVSEEGLK 180
           L+FGSTNIFVKIG+GA++GAVATALTNP+EVLKVRLQMNPNST+GPIKEMS+IVSEEGLK
Sbjct: 121 LVFGSTNIFVKIGSGAISGAVATALTNPVEVLKVRLQMNPNSTRGPIKEMSKIVSEEGLK 180

Query: 181 ALWKGVGPAMARAGALTASQLATYDESKQLLVKWTPLQEGFSLHLISSTVAGVVSTLMTT 240
           ALWKGVGPAMARAGALTASQLATYDE+KQ+LVKWTPL+EGF LHLISSTVAGVVSTLMTT
Sbjct: 181 ALWKGVGPAMARAGALTASQLATYDEAKQVLVKWTPLEEGFPLHLISSTVAGVVSTLMTT 240

Query: 241 PIDMIKTRLMLQRESKRVGNYKNGLHCAYQVVLTEGPLALYKGGLAIFARLGPQTTITFI 300
           PIDM+KTRLMLQRESKR G+YK+GLHCAYQV+ TEGPLALYKGGLAIFARLGPQTTITFI
Sbjct: 241 PIDMVKTRLMLQRESKRAGSYKSGLHCAYQVIRTEGPLALYKGGLAIFARLGPQTTITFI 300

Query: 301 VCEKLRQLAGLNAI 314
           VCEKLRQLAGLNAI
Sbjct: 301 VCEKLRQLAGLNAI 313

BLAST of Chy1G016040 vs. TAIR 10
Match: AT4G03115.1 (Mitochondrial substrate carrier family protein )

HSP 1 Score: 405.2 bits (1040), Expect = 4.8e-113
Identity = 214/311 (68.81%), Postives = 250/311 (80.39%), Query Frame = 0

Query: 7   SPQESSISGVLPRNERR---NWAASPSDVLNHFGTSGLSVAIATAITHPLDVLKVRLQMQ 66
           SP  +SI  V    E R   N     S V++HFG SG+SVA+AT +THPLDV+KVRLQMQ
Sbjct: 6   SPPVTSIPVVEGNEELRKPQNLIPPFSKVVSHFGISGISVALATGVTHPLDVVKVRLQMQ 65

Query: 67  LVGQRGPLIGMGQVFVQLLNNEGPRSLYLGLSPALTRSVLYGGLRLGLYEPSKHASDLLF 126
            VGQRGPLIGM  +F+QL+ NEG RSLYLGL+PALTRSVLYGGLRLGLYEP+K + D  F
Sbjct: 66  HVGQRGPLIGMTGIFLQLMKNEGRRSLYLGLTPALTRSVLYGGLRLGLYEPTKVSFDWAF 125

Query: 127 GSTNIFVKIGAGALAGAVATALTNPIEVLKVRLQMNPNSTKGPIKEMSRIVSEEGLKALW 186
           GSTN+ VKI +GA AGA +TALTNP+EV+KVRLQMNPN+   PI E+  IVS+EG+ ALW
Sbjct: 126 GSTNVLVKIASGAFAGAFSTALTNPVEVVKVRLQMNPNAV--PIAEVREIVSKEGIGALW 185

Query: 187 KGVGPAMARAGALTASQLATYDESKQLLVKWTPLQEGFSLHLISSTVAGVVSTLMTTPID 246
           KGVGPAM RA ALTASQLATYDE+K++LVK T L+EGF LHL SS VAG+VSTL+T P+D
Sbjct: 186 KGVGPAMVRAAALTASQLATYDEAKRILVKRTSLEEGFHLHLCSSVVAGLVSTLITAPMD 245

Query: 247 MIKTRLMLQRESKRVGNYKNGLHCAYQVVLTEGPLALYKGGLAIFARLGPQTTITFIVCE 306
           MIKTRLMLQ+ S+    Y+NG HC Y+VV  EGPLALYKGG AIFARLGPQT ITFI+CE
Sbjct: 246 MIKTRLMLQQGSESTKTYRNGFHCGYKVVRKEGPLALYKGGFAIFARLGPQTMITFILCE 305

Query: 307 KLRQLAGLNAI 315
           KLR LAGL+ +
Sbjct: 306 KLRSLAGLHTM 314

BLAST of Chy1G016040 vs. TAIR 10
Match: AT3G54110.1 (plant uncoupling mitochondrial protein 1 )

HSP 1 Score: 159.5 bits (402), Expect = 4.5e-39
Identity = 99/298 (33.22%), Postives = 154/298 (51.68%), Query Frame = 0

Query: 26  AASPSDVL--NHFGTSGLSVAIATAITHPLDVLKVRLQMQLVGQRGPLI-----GMGQVF 85
           AA  SD+     F  S  +  +    T PLD  KVRLQ+Q     G +      G+    
Sbjct: 3   AAGKSDLSLPKTFACSAFAACVGEVCTIPLDTAKVRLQLQKSALAGDVTLPKYRGLLGTV 62

Query: 86  VQLLNNEGPRSLYLGLSPALTRSVLYGGLRLGLYEPSK--HASDLLFGSTNIFVKIGAGA 145
             +   EG RSL+ G+ P L R  L+GGLR+G+YEP K  +      G   +  KI AG 
Sbjct: 63  GTIAREEGLRSLWKGVVPGLHRQCLFGGLRIGMYEPVKNLYVGKDFVGDVPLSKKILAGL 122

Query: 146 LAGAVATALTNPIEVLKVRLQMN-------PNSTKGPIKEMSRIVSEEGLKALWKGVGPA 205
             GA+   + NP +++KVRLQ         P    G +   S IV +EG++ALW G+GP 
Sbjct: 123 TTGALGIMVANPTDLVKVRLQAEGKLAAGAPRRYSGALNAYSTIVRQEGVRALWTGLGPN 182

Query: 206 MARAGALTASQLATYDESKQLLVKWTPLQEGFSLHLISSTVAGVVSTLMTTPIDMIKTRL 265
           +AR   + A++LA+YD+ K+ ++K     +    H++S   AG  +  + +P+D++K+R+
Sbjct: 183 VARNAIINAAELASYDQVKETILKIPGFTDNVVTHILSGLGAGFFAVCIGSPVDVVKSRM 242

Query: 266 MLQRESKRVGNYKNGLHCAYQVVLTEGPLALYKGGLAIFARLGPQTTITFIVCEKLRQ 308
           M        G YK  + C  + + ++GP+A YKG +  F RLG    I F+  E+ ++
Sbjct: 243 MGDS-----GAYKGTIDCFVKTLKSDGPMAFYKGFIPNFGRLGSWNVIMFLTLEQAKK 295

BLAST of Chy1G016040 vs. TAIR 10
Match: AT2G22500.1 (uncoupling protein 5 )

HSP 1 Score: 157.1 bits (396), Expect = 2.3e-38
Identity = 98/307 (31.92%), Postives = 168/307 (54.72%), Query Frame = 0

Query: 33  LNHFGTSGLSVAIATAITHPLDVLKVRLQMQLVGQRGPL--------------------- 92
           L  F   G++  +A   THPLD++KVR+Q+Q  G+  P+                     
Sbjct: 3   LKGFAEGGIASIVAGCSTHPLDLIKVRMQLQ--GESAPIQTNLRPALAFQTSTTVNAPPL 62

Query: 93  -IGMGQVFVQLLNNEGPRSLYLGLSPALTRSVLYGGLRLGLYEPSK-HASDLLFGSTNIF 152
            +G+  V  +L+  EG R+L+ G+S  + R  LY   R+GLY+  K   +D    +  + 
Sbjct: 63  RVGVIGVGSRLIREEGMRALFSGVSATVLRQTLYSTTRMGLYDIIKGEWTDPETKTMPLM 122

Query: 153 VKIGAGALAGAVATALTNPIEVLKVRLQ-------MNPNSTKGPIKEMSRIVSEEGLKAL 212
            KIGAGA+AGA+  A+ NP +V  VR+Q        +  + K  +  +++++  EG+ +L
Sbjct: 123 KKIGAGAIAGAIGAAVGNPADVAMVRMQADGRLPLTDRRNYKSVLDAITQMIRGEGVTSL 182

Query: 213 WKGVGPAMARAGALTASQLATYDESKQLLVKWTPLQEGFSLHLISSTVAGVVSTLMTTPI 272
           W+G    + RA  +T+SQLA+YD  K+ +++   L++G   H+ +S  AG V+++ + P+
Sbjct: 183 WRGSSLTINRAMLVTSSQLASYDSVKETILEKGLLKDGLGTHVSASFAAGFVASVASNPV 242

Query: 273 DMIKTRLMLQRESKRVG-NYKNGLHCAYQVVLTEGPLALYKGGLAIFARLGPQTTITFIV 309
           D+IKTR+M  +    V   YK  + CA + V  EG ++LYKG +   +R  P T + F+ 
Sbjct: 243 DVIKTRVMNMKVVAGVAPPYKGAVDCALKTVKAEGIMSLYKGFIPTVSRQAPFTVVLFVT 302

BLAST of Chy1G016040 vs. TAIR 10
Match: AT4G24570.1 (dicarboxylate carrier 2 )

HSP 1 Score: 155.2 bits (391), Expect = 8.6e-38
Identity = 99/308 (32.14%), Postives = 160/308 (51.95%), Query Frame = 0

Query: 36  FGTSGLSVAIATAITHPLDVLKVRLQMQ---------------------------LVGQR 95
           F   G++  IA   THPLD++KVRLQ+                                 
Sbjct: 6   FVEGGIASVIAGCSTHPLDLIKVRLQLHGEAPSTTTVTLLRPALAFPNSSPAAFLETTSS 65

Query: 96  GPLIGMGQVFVQLLNNEGPRSLYLGLSPALTRSVLYGGLRLGLYEPSKHA-SDLLFGSTN 155
            P +G   + + ++ +EG  +L+ G+S  L R  LY   R+GLYE  K+  +D   G  N
Sbjct: 66  VPKVGPISLGINIVKSEGAAALFSGVSATLLRQTLYSTTRMGLYEVLKNKWTDPESGKLN 125

Query: 156 IFVKIGAGALAGAVATALTNPIEVLKVRLQMN-------PNSTKGPIKEMSRIVSEEGLK 215
           +  KIGAG +AG +  A+ NP +V  VR+Q +         +  G    +  +V  EG+ 
Sbjct: 126 LSRKIGAGLVAGGIGAAVGNPADVAMVRMQADGRLPLAQRRNYAGVGDAIRSMVKGEGVT 185

Query: 216 ALWKGVGPAMARAGALTASQLATYDESKQLLVKWTPLQEGFSLHLISSTVAGVVSTLMTT 275
           +LW+G    + RA  +TA+QLA+YD+ K+ +++   + +G   H+++S  AG V+++ + 
Sbjct: 186 SLWRGSALTINRAMIVTAAQLASYDQFKEGILENGVMNDGLGTHVVASFAAGFVASVASN 245

Query: 276 PIDMIKTRLMLQRESKRVGNYKNGLHCAYQVVLTEGPLALYKGGLAIFARLGPQTTITFI 309
           P+D+IKTR+M    + +VG Y     CA + V  EG +ALYKG +    R GP T + F+
Sbjct: 246 PVDVIKTRVM----NMKVGAYDGAWDCAVKTVKAEGAMALYKGFVPTVCRQGPFTVVLFV 305

BLAST of Chy1G016040 vs. TAIR 10
Match: AT5G09470.1 (dicarboxylate carrier 3 )

HSP 1 Score: 152.9 bits (385), Expect = 4.3e-37
Identity = 109/332 (32.83%), Postives = 166/332 (50.00%), Query Frame = 0

Query: 36  FGTSGLSVAIATAITHPLDVLKVRLQMQ-------------------------------- 95
           F   G++  IA A+THPLD++KVR+Q+Q                                
Sbjct: 6   FLEGGIAAIIAGALTHPLDLIKVRMQLQGEHSFSLDQNPNPNLSLDHNLPVKPYRPVFAL 65

Query: 96  --LVGQ--------RGPLIGMGQVF------VQLLNNEGPRSLYLGLSPALTRSVLYGGL 155
             L+G           P      V         ++  EGP +L+ G+S  + R +LY   
Sbjct: 66  DSLIGSISLLPLHIHAPSSSTRSVMTPFAVGAHIVKTEGPAALFSGVSATILRQMLYSAT 125

Query: 156 RLGLYEPSKHA-SDLLFGSTNIFVKIGAGALAGAVATALTNPIEVLKVRLQMNPN----- 215
           R+G+Y+  K   +D L G+  +  KI AG +AGAV + + NP +V  VR+Q + +     
Sbjct: 126 RMGIYDFLKRRWTDQLTGNFPLVTKITAGLIAGAVGSVVGNPADVAMVRMQADGSLPLNR 185

Query: 216 --STKGPIKEMSRIVSEEGLKALWKGVGPAMARAGALTASQLATYDESKQLLV---KWTP 275
             + K  +  + RI  +EG+ +LW+G    + RA  +TASQLATYD  K++LV   + TP
Sbjct: 186 RRNYKSVVDAIDRIARQEGVSSLWRGSWLTVNRAMIVTASQLATYDHVKEILVAGGRGTP 245

Query: 276 LQEGFSLHLISSTVAGVVSTLMTTPIDMIKTRLMLQRESKRVGNYKNGLHCAYQVVLTEG 309
              G   H+ +S  AG+V+ + + PID++KTR+M     K +  Y   L CA ++V  EG
Sbjct: 246 --GGIGTHVAASFAAGIVAAVASNPIDVVKTRMM--NADKEI--YGGPLDCAVKMVAEEG 305

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
B0G1434.4e-4737.50Mitochondrial substrate carrier family protein ucpB OS=Dictyostelium discoideum ... [more]
O818456.4e-3833.22Mitochondrial uncoupling protein 1 OS=Arabidopsis thaliana OX=3702 GN=PUMP1 PE=1... [more]
O975626.4e-3835.11Mitochondrial uncoupling protein 2 OS=Sus scrofa OX=9823 GN=UCP2 PE=2 SV=1[more]
Q9SJY53.2e-3731.92Mitochondrial uncoupling protein 5 OS=Arabidopsis thaliana OX=3702 GN=PUMP5 PE=2... [more]
Q54PY74.1e-3736.39Probable mitochondrial 2-oxoglutarate/malate carrier protein OS=Dictyostelium di... [more]
Match NameE-valueIdentityDescription
A0A1S3BSK02.4e-16596.50mitochondrial substrate carrier family protein ucpB isoform X1 OS=Cucumis melo O... [more]
A0A6J1KWR86.6e-15590.13mitochondrial substrate carrier family protein ucpB isoform X1 OS=Cucurbita maxi... [more]
A0A6J1H6906.6e-15590.13mitochondrial substrate carrier family protein ucpB isoform X1 OS=Cucurbita mosc... [more]
A0A6J1DA089.6e-15490.45mitochondrial substrate carrier family protein ucpB isoform X1 OS=Momordica char... [more]
A0A0A0K8Y51.9e-13898.08Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G390160 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_004148885.26.98e-21898.09mitochondrial substrate carrier family protein ucpB isoform X1 [Cucumis sativus]... [more]
XP_008451426.12.61e-21396.50PREDICTED: mitochondrial substrate carrier family protein ucpB isoform X1 [Cucum... [more]
XP_031744482.16.44e-21396.82mitochondrial substrate carrier family protein ucpB isoform X2 [Cucumis sativus][more]
XP_038896316.16.06e-20593.95mitochondrial substrate carrier family protein ucpB isoform X1 [Benincasa hispid... [more]
XP_023004604.11.31e-19990.13mitochondrial substrate carrier family protein ucpB isoform X1 [Cucurbita maxima... [more]
Match NameE-valueIdentityDescription
AT4G03115.14.8e-11368.81Mitochondrial substrate carrier family protein [more]
AT3G54110.14.5e-3933.22plant uncoupling mitochondrial protein 1 [more]
AT2G22500.12.3e-3831.92uncoupling protein 5 [more]
AT4G24570.18.6e-3832.14dicarboxylate carrier 2 [more]
AT5G09470.14.3e-3732.83dicarboxylate carrier 3 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002067Mitochondrial carrier proteinPRINTSPR00926MITOCARRIERcoord: 141..159
score: 17.34
coord: 228..250
score: 25.02
coord: 35..48
score: 24.59
coord: 48..62
score: 26.67
IPR023395Mitochondrial carrier domain superfamilyGENE3D1.50.40.10Mitochondrial carrier domaincoord: 21..311
e-value: 4.6E-75
score: 254.1
IPR023395Mitochondrial carrier domain superfamilySUPERFAMILY103506Mitochondrial carriercoord: 30..305
IPR018108Mitochondrial substrate/solute carrierPFAMPF00153Mito_carrcoord: 126..213
e-value: 5.5E-20
score: 71.1
coord: 31..117
e-value: 7.0E-17
score: 61.1
coord: 220..308
e-value: 3.7E-19
score: 68.5
IPR018108Mitochondrial substrate/solute carrierPROSITEPS50920SOLCARcoord: 126..210
score: 25.259007
IPR018108Mitochondrial substrate/solute carrierPROSITEPS50920SOLCARcoord: 30..118
score: 20.603685
IPR018108Mitochondrial substrate/solute carrierPROSITEPS50920SOLCARcoord: 219..308
score: 23.371134
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..18
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..20
NoneNo IPR availablePANTHERPTHR45618MITOCHONDRIAL DICARBOXYLATE CARRIER-RELATEDcoord: 31..314
NoneNo IPR availablePANTHERPTHR45618:SF21MITOCHONDRIAL SUBSTRATE CARRIER FAMILY PROTEIN UCPBcoord: 31..314

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy1G016040.1Chy1G016040.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane