Chy1G015200 (gene) Cucumber (hystrix) v1

BLAST of Chy1G015200 vs. ExPASy Swiss-Prot
Match: P24397 (Hyoscyamine 6-dioxygenase OS=Hyoscyamus niger OX=4079 GN=H6H PE=1 SV=1)

HSP 1 Score: 268.1 bits (684), Expect = 1.4e-70
Identity = 141/329 (42.86%), Postives = 205/329 (62.31%), Query Frame = 0

Query: 6   AKFVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILDASKEF 65
           A FVSNW T +SV ES++ P +KR     VP+   +P+IDL  +   LLV++I  A ++F
Sbjct: 2   ATFVSNWST-KSVSESFIAPLQKR-AEKDVPVGNDVPIIDLQ-QHHHLLVQQITKACQDF 61

Query: 66  GFFQIINHGVSKKVSEETMRIFKEFHSMSGPEKAK-----ECSK---DPNRSCRVYTSSE 125
           G FQ+INHG  +++  ETM + KEF ++   EK K     E +K      +  ++Y   E
Sbjct: 62  GLFQVINHGFPEELMLETMEVCKEFFALPAEEKEKFKPKGEAAKFELPLEQKAKLYVEGE 121

Query: 126 NYTKEQIHCWRDALIFNCHPLEK-YVHFWPQKPPKYREVVGAYCIAMRKLVLEILELMSE 185
             + E+   W+D L   CHPL++  V+ WP+KP KYREVV  Y + +RKL + +L+ + E
Sbjct: 122 QLSNEEFLYWKDTLAHGCHPLDQDLVNSWPEKPAKYREVVAKYSVEVRKLTMRMLDYICE 181

Query: 186 GLGLGKGYFGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQ--VL 245
           GLGL  GYF  E+S+  ++L N+YPPCP+PS TLG   H D +LIT+L QD+ GLQ  ++
Sbjct: 182 GLGLKLGYFDNELSQIQMMLTNYYPPCPDPSSTLGSGGHYDGNLITLLQQDLPGLQQLIV 241

Query: 246 KDGQWIGVQPIDNAFVINIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAM 305
           KD  WI VQPI  AFV+N+G  L++ITN K + + HR VT+    R S+  L+ P     
Sbjct: 242 KDATWIAVQPIPTAFVVNLGLTLKVITNEKFEGSIHRVVTDPTRDRVSIATLIGPDYSCT 301

Query: 306 MEPAKCMINEANPPRYRSLNFKDFQRNYL 324
           +EPAK ++N+ NPP Y+  ++ +F   YL
Sbjct: 302 IEPAKELLNQDNPPLYKPYSYSEFADIYL 327

BLAST of Chy1G015200 vs. ExPASy Swiss-Prot
Match: Q9FLV0 (Protein DOWNY MILDEW RESISTANCE 6 OS=Arabidopsis thaliana OX=3702 GN=DMR6 PE=1 SV=1)

HSP 1 Score: 261.2 bits (666), Expect = 1.7e-68
Identity = 140/340 (41.18%), Postives = 205/340 (60.29%), Query Frame = 0

Query: 6   AKFVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILDASKEF 65
           AK +S      ++PE+YV P   RP    V   +  P+IDLS  DR+ L+++I  A   F
Sbjct: 3   AKLISTGFRHTTLPENYVRPISDRPRLSEVSQLEDFPLIDLSSTDRSFLIQQIHQACARF 62

Query: 66  GFFQIINHGVSKKVSEETMRIFKEFHSMSGPEKAKECSKDPNRSCRVYTSSENYTKEQIH 125
           GFFQ+INHGV+K++ +E + + +EF SMS  EK K  S DP ++ R+ TS  N  KE+++
Sbjct: 63  GFFQVINHGVNKQIIDEMVSVAREFFSMSMEEKMKLYSDDPTKTTRLSTSF-NVKKEEVN 122

Query: 126 CWRDALIFNCHPLEKYVHFWPQKPPKYREVVGAYCIAMRKLVLEILELMSEGLGLGKGYF 185
            WRD L  +C+P+ KYV+ WP  PP ++E+V  Y   +R++  +I EL+SE LGL K Y 
Sbjct: 123 NWRDYLRLHCYPIHKYVNEWPSNPPSFKEIVSKYSREVREVGFKIEELISESLGLEKDYM 182

Query: 186 GGEMSE-NPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQD--VNGLQVLKDGQWIGV 245
              + E    + VN+YPPCP P LT GL  H DP+ +TIL QD  V GLQ+L DGQW  V
Sbjct: 183 KKVLGEQGQHMAVNYYPPCPEPELTYGLPAHTDPNALTILLQDTTVCGLQILIDGQWFAV 242

Query: 246 QPIDNAFVINIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKCM- 305
            P  +AFVINIG  LQ ++NG  ++  HRAVTN++  R S+   + P D A+M PAK + 
Sbjct: 243 NPHPDAFVINIGDQLQALSNGVYKSVWHRAVTNTENPRLSVASFLCPADCAVMSPAKPLW 302

Query: 306 --INEANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETN 340
              ++   P Y+   + ++ + +  R +D +  ++    N
Sbjct: 303 EAEDDETKPVYKDFTYAEYYKKFWSRNLDQEHCLENFLNN 341

BLAST of Chy1G015200 vs. ExPASy Swiss-Prot
Match: Q8W2X5 (Flavanone 3-dioxygenase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=F3H-2 PE=1 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 5.1e-65
Identity = 128/326 (39.26%), Postives = 187/326 (57.36%), Query Frame = 0

Query: 16  ESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILDASKEFGFFQIINHGV 75
           +++P  YV P  +RP   +V     IPV+DL+  DR  +V  + DA +  GFFQ++NHG+
Sbjct: 18  DTMPGKYVRPESQRPRLDLVVSDARIPVVDLASPDRAAVVSAVGDACRTHGFFQVVNHGI 77

Query: 76  SKKVSEETMRIFKEFHSMSGPEKAKECSKDPNRSCRVYTSSENYTKEQIHCWRDALIFNC 135
              +    M + +EF  +   EKAK  S DP +  R+ TS  N  KE +H WRD L  +C
Sbjct: 78  DAALIASVMEVGREFFRLPAEEKAKLYSDDPAKKIRLSTSF-NVRKETVHNWRDYLRLHC 137

Query: 136 HPLEKYVHFWPQKPPKYREVVGAYCIAMRKLVLEILELMSEGLGLGKGYFGGEMSENPL- 195
           +PL ++V  WP  PP ++E++G YC  +R+L   + E +SE LGL  GY    + E    
Sbjct: 138 YPLHQFVPDWPSNPPSFKEIIGTYCTEVRELGFRLYEAISESLGLEGGYMRETLGEQEQH 197

Query: 196 LLVNHYPPCPNPSLTLGLSQHCDPSLITILFQD--VNGLQVLKDGQWIGVQPIDNAFVIN 255
           + VN+YP CP P LT GL  H DP+ +TIL  D  V GLQVL DG+WI V P   A VIN
Sbjct: 198 MAVNYYPQCPEPELTYGLPAHTDPNALTILLMDDQVAGLQVLNDGKWIAVNPQPGALVIN 257

Query: 256 IGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKCMINEANPPRYRS 315
           IG  LQ ++NGK ++  HRAV NS   R S+   + P +   + PAK +I + +P  YR+
Sbjct: 258 IGDQLQALSNGKYRSVWHRAVVNSDRERMSVASFLCPCNSVELGPAKKLITDDSPAVYRN 317

Query: 316 LNFKDFQRNYLPRAVDTKAVMQYIET 339
             + ++ + +  R +D +  ++   T
Sbjct: 318 YTYDEYYKKFWSRNLDQEHCLELFRT 342

BLAST of Chy1G015200 vs. ExPASy Swiss-Prot
Match: Q40062 (2'-deoxymugineic-acid 2'-dioxygenase OS=Hordeum vulgare OX=4513 GN=IDS3 PE=1 SV=3)

HSP 1 Score: 227.6 bits (579), Expect = 2.1e-58
Identity = 126/312 (40.38%), Postives = 185/312 (59.29%), Query Frame = 0

Query: 17  SVPESYVYPPEKRPGNIVVPMAKAI---PVIDLSIRDRTLLVRKILDASKEFGFFQIINH 76
           S+PES+V+  +K P     P  KA+   P+IDLS   R  + R IL+A KE GFFQ++NH
Sbjct: 13  SLPESFVFASDKVP-----PATKAVVSLPIIDLSC-GRDEVRRSILEAGKELGFFQVVNH 72

Query: 77  GVSKKVSEETMRIFKEFHSMSGPEKAKECSKDPNRSCRVYTSSENYTKEQIHCWRDALIF 136
           GVSK+V  +   + ++F  +   +KA   S++ ++  R++ S   Y       WRD L  
Sbjct: 73  GVSKQVMRDMEGMCEQFFHLPAADKASLYSEERHKPNRLF-SGATYDTGGEKYWRDCLRL 132

Query: 137 NC-HPLEKYVHFWPQKPPKYREVVGAYCIAMRKLVLEILELMSEGLGLGKGYFGGEMS-E 196
            C  P++  ++ WP  P   R+V+  +    R +  E+L L+ EG+G+   YF G++S  
Sbjct: 133 ACPFPVDDSINEWPDTPKGLRDVIEKFTSQTRDVGKELLRLLCEGMGIQADYFEGDLSGG 192

Query: 197 NPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQD-VNGLQVLKDGQWIGVQPIDNAFV 256
           N +L +NHYP CPNP   LG   HCD +LIT+L    VNGL+V   G WI V P  NAFV
Sbjct: 193 NVILNINHYPSCPNPDKALGQPPHCDRNLITLLLPGAVNGLEVSYKGDWIKVDPAPNAFV 252

Query: 257 INIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKCMINEANPPRY 316
           +N G  L+++TNG L++ EHRA+TNS  +R S+   + P  E ++ PAK  +++ NPP Y
Sbjct: 253 VNFGQQLEVVTNGLLKSIEHRAMTNSALARTSVATFIMPTQECLIGPAKEFLSKENPPCY 312

Query: 317 RSLNFKDFQRNY 323
           R+  F+DF R Y
Sbjct: 313 RTTMFRDFMRIY 317

BLAST of Chy1G015200 vs. ExPASy Swiss-Prot
Match: Q9ZSA7 (Protein DMR6-LIKE OXYGENASE 2 OS=Arabidopsis thaliana OX=3702 GN=DLO2 PE=2 SV=1)

HSP 1 Score: 226.1 bits (575), Expect = 6.0e-58
Identity = 124/325 (38.15%), Postives = 190/325 (58.46%), Query Frame = 0

Query: 15  VESVPESYVYPPEKRPG-NIVVPMAKAIPVIDLSI---RDRTLLVRKILDASKEFGFFQI 74
           V+ VP +YV P   RP  + V     +IP+IDL      +R  ++ +   A    GFFQI
Sbjct: 15  VDHVPSNYVRPVSDRPKMSEVQTSGDSIPLIDLHDLHGPNRADIINQFAHACSSCGFFQI 74

Query: 75  INHGVSKKVSEETMRIFKEFHSMSGPEKAKECSKDPNRSCRVYTSSENYTKEQIHCWRDA 134
            NHGV ++  ++ M   +EF   S  E+ K  S D  ++ R+ TS  N +KE++  WRD 
Sbjct: 75  KNHGVPEETIKKMMNAAREFFRQSESERVKHYSADTKKTTRLSTSF-NVSKEKVSNWRDF 134

Query: 135 LIFNCHPLEKYVHFWPQKPPKYREVVGAYCIAMRKLVLEILELMSEGLGLGKGYFGGEMS 194
           L  +C+P+E +++ WP  P  +REV   Y  ++R LVL +LE +SE LGL K      + 
Sbjct: 135 LRLHCYPIEDFINEWPSTPISFREVTAEYATSVRALVLTLLEAISESLGLAKDRVSNTIG 194

Query: 195 EN-PLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQD-VNGLQVLKDGQWIGVQPIDNA 254
           ++   + +N+YP CP P LT GL  H D +LIT+L QD V+GLQV KDG+WI V P+ N 
Sbjct: 195 KHGQHMAINYYPRCPQPELTYGLPGHKDANLITVLLQDEVSGLQVFKDGKWIAVNPVPNT 254

Query: 255 FVINIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKCMINEA--N 314
           F++N+G  +Q+I+N K ++  HRAV NS   R S+     P ++A++ PA+ +INE   +
Sbjct: 255 FIVNLGDQMQVISNEKYKSVLHRAVVNSDMERISIPTFYCPSEDAVISPAQELINEEEDS 314

Query: 315 PPRYRSLNFKDFQRNYLPRAVDTKA 332
           P  YR+  + ++   +   A DT++
Sbjct: 315 PAIYRNFTYAEYFEKFWDTAFDTES 338

BLAST of Chy1G015200 vs. ExPASy TrEMBL
Match: A0A0A0K5H3 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G378370 PE=3 SV=1)

HSP 1 Score: 700.7 bits (1807), Expect = 3.1e-198
Identity = 337/341 (98.83%), Postives = 341/341 (100.00%), Query Frame = 0

Query: 1   MLQGGAKFVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILD 60
           MLQGGAKFVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILD
Sbjct: 1   MLQGGAKFVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILD 60

Query: 61  ASKEFGFFQIINHGVSKKVSEETMRIFKEFHSMSGPEKAKECSKDPNRSCRVYTSSENYT 120
           ASKEFGFFQIINHGVSKKVSEETMRIFKEFH+MSGPEKAKECSKDPNRSCRVYTSSENYT
Sbjct: 61  ASKEFGFFQIINHGVSKKVSEETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYT 120

Query: 121 KEQIHCWRDALIFNCHPLEKYVHFWPQKPPKYREVVGAYCIAMRKLVLEILELMSEGLGL 180
           KEQ+HCWRDALIFNCHPLEKYVHFWPQKPPKYREVVGAYC+AMRKLVLEILELMSEGLGL
Sbjct: 121 KEQVHCWRDALIFNCHPLEKYVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMSEGLGL 180

Query: 181 GKGYFGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWI 240
           GKGYFGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWI
Sbjct: 181 GKGYFGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWI 240

Query: 241 GVQPIDNAFVINIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKC 300
           GVQPIDNAFV+NIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKC
Sbjct: 241 GVQPIDNAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKC 300

Query: 301 MINEANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQS 342
           MINEANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQS
Sbjct: 301 MINEANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQS 341

BLAST of Chy1G015200 vs. ExPASy TrEMBL
Match: A0A1S3BR20 (hyoscyamine 6-dioxygenase-like OS=Cucumis melo OX=3656 GN=LOC103492610 PE=3 SV=1)

HSP 1 Score: 669.5 bits (1726), Expect = 7.6e-189
Identity = 323/341 (94.72%), Postives = 328/341 (96.19%), Query Frame = 0

Query: 1   MLQGGAKFVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILD 60
           M QG AKFVSNWET ESVPESYVYPPEKRPGN+VVPMAKAIPVIDLSI DR LLVRKIL 
Sbjct: 1   MQQGDAKFVSNWETAESVPESYVYPPEKRPGNVVVPMAKAIPVIDLSICDRALLVRKILG 60

Query: 61  ASKEFGFFQIINHGVSKKVSEETMRIFKEFHSMSGPEKAKECSKDPNRSCRVYTSSENYT 120
           ASKE GFFQIINHGVSK+VSE TMRIFKEFH+MSGPEKAKECSKDPNRSCRVYTSSENY 
Sbjct: 61  ASKESGFFQIINHGVSKRVSEATMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYK 120

Query: 121 KEQIHCWRDALIFNCHPLEKYVHFWPQKPPKYREVVGAYCIAMRKLVLEILELMSEGLGL 180
           KEQIHCWRDALIFNCHPLEKYVHFWPQ PPKYREVVGAYC+AMRKLVLEILELMSEGLGL
Sbjct: 121 KEQIHCWRDALIFNCHPLEKYVHFWPQNPPKYREVVGAYCVAMRKLVLEILELMSEGLGL 180

Query: 181 GKGYFGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWI 240
           GKGY GGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWI
Sbjct: 181 GKGYLGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWI 240

Query: 241 GVQPIDNAFVINIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKC 300
           GVQPID AFV+NIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEA MEPAKC
Sbjct: 241 GVQPIDKAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEATMEPAKC 300

Query: 301 MINEANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQS 342
           MINEANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQS
Sbjct: 301 MINEANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQS 341

BLAST of Chy1G015200 vs. ExPASy TrEMBL
Match: A0A6J1KW36 (hyoscyamine 6-dioxygenase-like OS=Cucurbita maxima OX=3661 GN=LOC111497716 PE=3 SV=1)

HSP 1 Score: 605.5 bits (1560), Expect = 1.4e-169
Identity = 284/338 (84.02%), Postives = 310/338 (91.72%), Query Frame = 0

Query: 4   GGAKFVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILDASK 63
           G  KFVSNWE V+SVP  Y+YPPEKRPGN+VVPMA AIPV+DLS RDR LLVRKILDAS+
Sbjct: 2   GEVKFVSNWEGVQSVPPDYIYPPEKRPGNVVVPMANAIPVVDLSTRDRPLLVRKILDASQ 61

Query: 64  EFGFFQIINHGVSKKVSEETMRIFKEFHSMSGPEKAKECSKDPNRSCRVYTSSENYTKEQ 123
           EFGFFQ+INHGVS++V EET RIFK+FH+MSG EKAKECS+DP +SCRVYTSSENY KEQ
Sbjct: 62  EFGFFQVINHGVSRRVLEETTRIFKKFHAMSGAEKAKECSRDPYKSCRVYTSSENYRKEQ 121

Query: 124 IHCWRDALIFNCHPLEKYVHFWPQKPPKYREVVGAYCIAMRKLVLEILELMSEGLGLGKG 183
            HCWRDALIFNCHPLEKYVHFWPQ PPKYREVVGAYC+AMR LVLEILEL+SEGLGLG+G
Sbjct: 122 THCWRDALIFNCHPLEKYVHFWPQNPPKYREVVGAYCVAMRTLVLEILELVSEGLGLGRG 181

Query: 184 YFGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWIGVQ 243
           Y   EMSENPL+LVNHYPPCPNPSLTLGLSQHCDPSLITILFQ+VNGLQVLKDGQWIGV 
Sbjct: 182 YLASEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGVH 241

Query: 244 PIDNAFVINIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKCMIN 303
           PIDNAFV+NIGFVLQ+I+NGKL+AAEHRAVTN+K SRQSLTYLVYPKDE  MEPAKC+IN
Sbjct: 242 PIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLIN 301

Query: 304 EANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQS 342
           EANPPRYR+L FKDFQRNYLPRA DTKAVM+ I +N S
Sbjct: 302 EANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSNCS 339

BLAST of Chy1G015200 vs. ExPASy TrEMBL
Match: A0A6J1H5H6 (hyoscyamine 6-dioxygenase-like OS=Cucurbita moschata OX=3662 GN=LOC111460677 PE=3 SV=1)

HSP 1 Score: 602.4 bits (1552), Expect = 1.1e-168
Identity = 282/337 (83.68%), Postives = 309/337 (91.69%), Query Frame = 0

Query: 4   GGAKFVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILDASK 63
           G  KFVSNWE V+SVP  YVYPPEKRPGN+VVPMA AIPV+DLS  DR LLVRKILDAS+
Sbjct: 2   GEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANAIPVLDLSTHDRPLLVRKILDASQ 61

Query: 64  EFGFFQIINHGVSKKVSEETMRIFKEFHSMSGPEKAKECSKDPNRSCRVYTSSENYTKEQ 123
           EFGFFQ+INHGVS++V EET RIFK+FH+MSG EK KECS+DPN+SCRVYTSSENY KEQ
Sbjct: 62  EFGFFQVINHGVSRRVLEETTRIFKKFHAMSGAEKVKECSRDPNKSCRVYTSSENYRKEQ 121

Query: 124 IHCWRDALIFNCHPLEKYVHFWPQKPPKYREVVGAYCIAMRKLVLEILELMSEGLGLGKG 183
            HCWRDALIFNCHPL+KYVHFWPQ PPKYREVVGAYCIAMR LVLEILEL+SEGLGLG+G
Sbjct: 122 THCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGRG 181

Query: 184 YFGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWIGVQ 243
           Y GGEMSENPL+LVNHYP CPNPSLTLGLSQHCDPSLITILFQ+VNGLQVLKDGQWIGV 
Sbjct: 182 YLGGEMSENPLMLVNHYPSCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGVH 241

Query: 244 PIDNAFVINIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKCMIN 303
           PIDNAFV+NIGFVLQ+I+NGKL+AAEHR VTN+K SRQSLTYLVYPKDE  MEPAKC+IN
Sbjct: 242 PIDNAFVVNIGFVLQVISNGKLKAAEHRVVTNAKISRQSLTYLVYPKDEVTMEPAKCLIN 301

Query: 304 EANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQ 341
           EANPPRYR+L FKDFQRNYLPRA DTKAVM+ I +++
Sbjct: 302 EANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSDR 338

BLAST of Chy1G015200 vs. ExPASy TrEMBL
Match: A0A6J1CP90 (hyoscyamine 6-dioxygenase-like OS=Momordica charantia OX=3673 GN=LOC111013466 PE=3 SV=1)

HSP 1 Score: 585.9 bits (1509), Expect = 1.1e-163
Identity = 271/334 (81.14%), Postives = 300/334 (89.82%), Query Frame = 0

Query: 8   FVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILDASKEFGF 67
           FVSNW  VESVPESYVYPPEKRPGN+ VP+  AIPV+DL+  DR  LV KILDAS+EFGF
Sbjct: 7   FVSNWVGVESVPESYVYPPEKRPGNVAVPVENAIPVVDLTDHDRAFLVHKILDASQEFGF 66

Query: 68  FQIINHGVSKKVSEETMRIFKEFHSMSGPEKAKECSKDPNRSCRVYTSSENYTKEQIHCW 127
           FQ+INHGVSK + EETMR+F+EFH+MSGPEK +ECSKDPNRSCRVYTSSEN+ KE+ HCW
Sbjct: 67  FQVINHGVSKTLIEETMRVFEEFHAMSGPEKKRECSKDPNRSCRVYTSSENFVKEKTHCW 126

Query: 128 RDALIFNCHPLEKYVHFWPQKPPKYREVVGAYCIAMRKLVLEILELMSEGLGLGKGYFGG 187
           RDALIFNCHPL+KYVHFWPQ P  YREVVGAYCI MRKLV+EILEL+SEGLGLGKGYFG 
Sbjct: 127 RDALIFNCHPLDKYVHFWPQNPSNYREVVGAYCIVMRKLVVEILELISEGLGLGKGYFGD 186

Query: 188 EMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWIGVQPIDN 247
           EMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQ +NGLQVLKDGQWIGV P+DN
Sbjct: 187 EMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQQINGLQVLKDGQWIGVHPVDN 246

Query: 248 AFVINIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKCMINEANP 307
           AFV+NIGFVLQ+++NGKL+A EHRAV N+K SRQS+TYLVYPKD+A MEPAKCMINEANP
Sbjct: 247 AFVVNIGFVLQVVSNGKLKAGEHRAVINAKMSRQSITYLVYPKDDATMEPAKCMINEANP 306

Query: 308 PRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQS 342
             Y SL FKDFQRNYLP A DTKAV+QYI ++ S
Sbjct: 307 SLYHSLQFKDFQRNYLPMAADTKAVIQYISSDHS 340

BLAST of Chy1G015200 vs. NCBI nr
Match: XP_004146985.1 (hyoscyamine 6-dioxygenase [Cucumis sativus] >KGN44748.1 hypothetical protein Csa_016058 [Cucumis sativus])

HSP 1 Score: 696 bits (1795), Expect = 8.57e-253
Identity = 337/341 (98.83%), Postives = 341/341 (100.00%), Query Frame = 0

Query: 1   MLQGGAKFVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILD 60
           MLQGGAKFVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILD
Sbjct: 1   MLQGGAKFVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILD 60

Query: 61  ASKEFGFFQIINHGVSKKVSEETMRIFKEFHSMSGPEKAKECSKDPNRSCRVYTSSENYT 120
           ASKEFGFFQIINHGVSKKVSEETMRIFKEFH+MSGPEKAKECSKDPNRSCRVYTSSENYT
Sbjct: 61  ASKEFGFFQIINHGVSKKVSEETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYT 120

Query: 121 KEQIHCWRDALIFNCHPLEKYVHFWPQKPPKYREVVGAYCIAMRKLVLEILELMSEGLGL 180
           KEQ+HCWRDALIFNCHPLEKYVHFWPQKPPKYREVVGAYC+AMRKLVLEILELMSEGLGL
Sbjct: 121 KEQVHCWRDALIFNCHPLEKYVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMSEGLGL 180

Query: 181 GKGYFGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWI 240
           GKGYFGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWI
Sbjct: 181 GKGYFGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWI 240

Query: 241 GVQPIDNAFVINIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKC 300
           GVQPIDNAFV+NIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKC
Sbjct: 241 GVQPIDNAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKC 300

Query: 301 MINEANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQS 341
           MINEANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQS
Sbjct: 301 MINEANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQS 341

BLAST of Chy1G015200 vs. NCBI nr
Match: XP_008451261.1 (PREDICTED: hyoscyamine 6-dioxygenase-like [Cucumis melo])

HSP 1 Score: 664 bits (1714), Expect = 1.91e-240
Identity = 323/341 (94.72%), Postives = 328/341 (96.19%), Query Frame = 0

Query: 1   MLQGGAKFVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILD 60
           M QG AKFVSNWET ESVPESYVYPPEKRPGN+VVPMAKAIPVIDLSI DR LLVRKIL 
Sbjct: 1   MQQGDAKFVSNWETAESVPESYVYPPEKRPGNVVVPMAKAIPVIDLSICDRALLVRKILG 60

Query: 61  ASKEFGFFQIINHGVSKKVSEETMRIFKEFHSMSGPEKAKECSKDPNRSCRVYTSSENYT 120
           ASKE GFFQIINHGVSK+VSE TMRIFKEFH+MSGPEKAKECSKDPNRSCRVYTSSENY 
Sbjct: 61  ASKESGFFQIINHGVSKRVSEATMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYK 120

Query: 121 KEQIHCWRDALIFNCHPLEKYVHFWPQKPPKYREVVGAYCIAMRKLVLEILELMSEGLGL 180
           KEQIHCWRDALIFNCHPLEKYVHFWPQ PPKYREVVGAYC+AMRKLVLEILELMSEGLGL
Sbjct: 121 KEQIHCWRDALIFNCHPLEKYVHFWPQNPPKYREVVGAYCVAMRKLVLEILELMSEGLGL 180

Query: 181 GKGYFGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWI 240
           GKGY GGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWI
Sbjct: 181 GKGYLGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWI 240

Query: 241 GVQPIDNAFVINIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKC 300
           GVQPID AFV+NIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEA MEPAKC
Sbjct: 241 GVQPIDKAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEATMEPAKC 300

Query: 301 MINEANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQS 341
           MINEANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQS
Sbjct: 301 MINEANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQS 341

BLAST of Chy1G015200 vs. NCBI nr
Match: XP_038897058.1 (hyoscyamine 6-dioxygenase-like [Benincasa hispida])

HSP 1 Score: 642 bits (1657), Expect = 8.97e-232
Identity = 307/335 (91.64%), Postives = 323/335 (96.42%), Query Frame = 0

Query: 7   KFVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILDASKEFG 66
           KFV+NWE V+SVPESYVYPP KRPGN+VVPM  AIPVIDLS++DR LL+RKILDASKEFG
Sbjct: 6   KFVANWEGVKSVPESYVYPPGKRPGNVVVPMENAIPVIDLSMQDRNLLIRKILDASKEFG 65

Query: 67  FFQIINHGVSKKVSEETMRIFKEFHSMSGPEKAKECSKDPNRSCRVYTSSENYTKEQIHC 126
           FFQ+INHGVSK VS ETMRIFKEFH+MSGPEKAKECSKDPNRSCRVYTSSENY KEQIHC
Sbjct: 66  FFQVINHGVSKTVSGETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYMKEQIHC 125

Query: 127 WRDALIFNCHPLEKYVHFWPQKPPKYREVVGAYCIAMRKLVLEILELMSEGLGLGKGYFG 186
           WRDALIFNCHPLEKYVHFWPQ PPKYREVVGAYC+AMRKLVLEILELMSEGLGLGKGYFG
Sbjct: 126 WRDALIFNCHPLEKYVHFWPQNPPKYREVVGAYCVAMRKLVLEILELMSEGLGLGKGYFG 185

Query: 187 GEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWIGVQPID 246
           GEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDG+WIGVQPID
Sbjct: 186 GEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGRWIGVQPID 245

Query: 247 NAFVINIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKCMINEAN 306
           NAFV+NIGFVLQ+ITNGKL+AAEHRAVTN+KTSRQSLTYLVYPKDEA MEPAKCMINEAN
Sbjct: 246 NAFVVNIGFVLQVITNGKLKAAEHRAVTNAKTSRQSLTYLVYPKDEATMEPAKCMINEAN 305

Query: 307 PPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQS 341
           P RYRSLNFKDFQRNYLPRAVDTKAVM+YI +NQS
Sbjct: 306 PRRYRSLNFKDFQRNYLPRAVDTKAVMEYIGSNQS 340

BLAST of Chy1G015200 vs. NCBI nr
Match: XP_023514953.1 (hyoscyamine 6-dioxygenase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 605 bits (1559), Expect = 7.42e-217
Identity = 285/338 (84.32%), Postives = 312/338 (92.31%), Query Frame = 0

Query: 4   GGAKFVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILDASK 63
           G  KFVSNWE V+SVP  YVYPPEKRPGN+VVPMA  IPV+DLS RDR LLVRKILDAS+
Sbjct: 2   GEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANTIPVLDLSTRDRPLLVRKILDASQ 61

Query: 64  EFGFFQIINHGVSKKVSEETMRIFKEFHSMSGPEKAKECSKDPNRSCRVYTSSENYTKEQ 123
           EFGFFQ+INHGVSK+V EET RIFK+FH+MSG EKAKECS+DPN+SCRVYTSSENY KEQ
Sbjct: 62  EFGFFQVINHGVSKRVLEETTRIFKKFHAMSGAEKAKECSRDPNKSCRVYTSSENYRKEQ 121

Query: 124 IHCWRDALIFNCHPLEKYVHFWPQKPPKYREVVGAYCIAMRKLVLEILELMSEGLGLGKG 183
            HCWRDALIFNCHPL+KYVHFWPQ PPKYREVVGAYC+ MR LVLEILEL+SEGLGLG+G
Sbjct: 122 THCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCVEMRALVLEILELVSEGLGLGRG 181

Query: 184 YFGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWIGVQ 243
           Y GGEMSENPL+LVNHYPPCPNPSLTLGLSQHCDPSLITILFQ+VNGLQVLKDGQWIGV 
Sbjct: 182 YLGGEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGVH 241

Query: 244 PIDNAFVINIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKCMIN 303
           PIDNAFV+NIGFVLQ+I+NGKL+AAEHRAVTN+K SRQSLTYLVYPKDE  MEPAKC+IN
Sbjct: 242 PIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLIN 301

Query: 304 EANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQS 341
           EANPPRYR+L FKDFQRNYLPRA DTKAVM+ I +++S
Sbjct: 302 EANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSDRS 339

BLAST of Chy1G015200 vs. NCBI nr
Match: KAG6593078.1 (Hyoscyamine 6-dioxygenase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 603 bits (1554), Expect = 4.45e-216
Identity = 284/337 (84.27%), Postives = 311/337 (92.28%), Query Frame = 0

Query: 4   GGAKFVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILDASK 63
           G  KFVSNWE V+SVP  YVYPPEKRPGN+VVPMA AIPV+DLS RDR LLVRKILDAS+
Sbjct: 2   GEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANAIPVLDLSTRDRPLLVRKILDASQ 61

Query: 64  EFGFFQIINHGVSKKVSEETMRIFKEFHSMSGPEKAKECSKDPNRSCRVYTSSENYTKEQ 123
           EFGFFQ+INHGVS++V EET RIFK+FH+MSG EK KECS+DPN+SCRVYTSSENY KEQ
Sbjct: 62  EFGFFQVINHGVSRRVLEETTRIFKKFHAMSGAEKVKECSRDPNKSCRVYTSSENYRKEQ 121

Query: 124 IHCWRDALIFNCHPLEKYVHFWPQKPPKYREVVGAYCIAMRKLVLEILELMSEGLGLGKG 183
            HCWRDALIFNCHPL+KYVHFWPQ PPKYREVVGAYCIAMR LVLEILEL+SEGLGLG+G
Sbjct: 122 THCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGRG 181

Query: 184 YFGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWIGVQ 243
           Y GGEMSENPL+LVNHYPPCPNPSLTLGLSQHCDPSLITILFQ+VNGLQVLKDGQWIGV 
Sbjct: 182 YLGGEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGVH 241

Query: 244 PIDNAFVINIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKCMIN 303
           PIDNAFV+NIGFVLQ+I+NGKL+AAEHR VTN+K SRQSLTYLVYPKDE  MEPAKC+IN
Sbjct: 242 PIDNAFVVNIGFVLQVISNGKLKAAEHRVVTNAKISRQSLTYLVYPKDEVTMEPAKCLIN 301

Query: 304 EANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQ 340
           EANPPRYR+L FKDFQRNYLPRA DTKAVM+ I +++
Sbjct: 302 EANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSDR 338

BLAST of Chy1G015200 vs. TAIR 10
Match: AT5G24530.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 261.2 bits (666), Expect = 1.2e-69
Identity = 140/340 (41.18%), Postives = 205/340 (60.29%), Query Frame = 0

Query: 6   AKFVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILDASKEF 65
           AK +S      ++PE+YV P   RP    V   +  P+IDLS  DR+ L+++I  A   F
Sbjct: 3   AKLISTGFRHTTLPENYVRPISDRPRLSEVSQLEDFPLIDLSSTDRSFLIQQIHQACARF 62

Query: 66  GFFQIINHGVSKKVSEETMRIFKEFHSMSGPEKAKECSKDPNRSCRVYTSSENYTKEQIH 125
           GFFQ+INHGV+K++ +E + + +EF SMS  EK K  S DP ++ R+ TS  N  KE+++
Sbjct: 63  GFFQVINHGVNKQIIDEMVSVAREFFSMSMEEKMKLYSDDPTKTTRLSTSF-NVKKEEVN 122

Query: 126 CWRDALIFNCHPLEKYVHFWPQKPPKYREVVGAYCIAMRKLVLEILELMSEGLGLGKGYF 185
            WRD L  +C+P+ KYV+ WP  PP ++E+V  Y   +R++  +I EL+SE LGL K Y 
Sbjct: 123 NWRDYLRLHCYPIHKYVNEWPSNPPSFKEIVSKYSREVREVGFKIEELISESLGLEKDYM 182

Query: 186 GGEMSE-NPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQD--VNGLQVLKDGQWIGV 245
              + E    + VN+YPPCP P LT GL  H DP+ +TIL QD  V GLQ+L DGQW  V
Sbjct: 183 KKVLGEQGQHMAVNYYPPCPEPELTYGLPAHTDPNALTILLQDTTVCGLQILIDGQWFAV 242

Query: 246 QPIDNAFVINIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKCM- 305
            P  +AFVINIG  LQ ++NG  ++  HRAVTN++  R S+   + P D A+M PAK + 
Sbjct: 243 NPHPDAFVINIGDQLQALSNGVYKSVWHRAVTNTENPRLSVASFLCPADCAVMSPAKPLW 302

Query: 306 --INEANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETN 340
              ++   P Y+   + ++ + +  R +D +  ++    N
Sbjct: 303 EAEDDETKPVYKDFTYAEYYKKFWSRNLDQEHCLENFLNN 341

BLAST of Chy1G015200 vs. TAIR 10
Match: AT4G10490.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 226.1 bits (575), Expect = 4.3e-59
Identity = 124/325 (38.15%), Postives = 190/325 (58.46%), Query Frame = 0

Query: 15  VESVPESYVYPPEKRPG-NIVVPMAKAIPVIDLSI---RDRTLLVRKILDASKEFGFFQI 74
           V+ VP +YV P   RP  + V     +IP+IDL      +R  ++ +   A    GFFQI
Sbjct: 15  VDHVPSNYVRPVSDRPKMSEVQTSGDSIPLIDLHDLHGPNRADIINQFAHACSSCGFFQI 74

Query: 75  INHGVSKKVSEETMRIFKEFHSMSGPEKAKECSKDPNRSCRVYTSSENYTKEQIHCWRDA 134
            NHGV ++  ++ M   +EF   S  E+ K  S D  ++ R+ TS  N +KE++  WRD 
Sbjct: 75  KNHGVPEETIKKMMNAAREFFRQSESERVKHYSADTKKTTRLSTSF-NVSKEKVSNWRDF 134

Query: 135 LIFNCHPLEKYVHFWPQKPPKYREVVGAYCIAMRKLVLEILELMSEGLGLGKGYFGGEMS 194
           L  +C+P+E +++ WP  P  +REV   Y  ++R LVL +LE +SE LGL K      + 
Sbjct: 135 LRLHCYPIEDFINEWPSTPISFREVTAEYATSVRALVLTLLEAISESLGLAKDRVSNTIG 194

Query: 195 EN-PLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQD-VNGLQVLKDGQWIGVQPIDNA 254
           ++   + +N+YP CP P LT GL  H D +LIT+L QD V+GLQV KDG+WI V P+ N 
Sbjct: 195 KHGQHMAINYYPRCPQPELTYGLPGHKDANLITVLLQDEVSGLQVFKDGKWIAVNPVPNT 254

Query: 255 FVINIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKCMINEA--N 314
           F++N+G  +Q+I+N K ++  HRAV NS   R S+     P ++A++ PA+ +INE   +
Sbjct: 255 FIVNLGDQMQVISNEKYKSVLHRAVVNSDMERISIPTFYCPSEDAVISPAQELINEEEDS 314

Query: 315 PPRYRSLNFKDFQRNYLPRAVDTKA 332
           P  YR+  + ++   +   A DT++
Sbjct: 315 PAIYRNFTYAEYFEKFWDTAFDTES 338

BLAST of Chy1G015200 vs. TAIR 10
Match: AT4G10500.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 219.9 bits (559), Expect = 3.1e-57
Identity = 121/334 (36.23%), Postives = 191/334 (57.19%), Query Frame = 0

Query: 9   VSNWETVESVPESYVYPPEKRPG-NIVVPMAKAIPVIDLSIRD-----RTLLVRKILDAS 68
           VS++ +   +P +YV P   RP  + V     +IP+IDL  RD     R ++V+++  A 
Sbjct: 11  VSDFASSVHIPSNYVRPISDRPNLSEVESSGDSIPLIDL--RDLHGPNRAVIVQQLASAC 70

Query: 69  KEFGFFQIINHGVSKKVSEETMRIFKEFHSMSGPEKAKECSKDPNRSCRVYTSSENYTKE 128
             +GFFQI NHGV      +   + +EF      E+ K  S DP ++ R+ TS  N   +
Sbjct: 71  STYGFFQIKNHGVPDTTVNKMQTVAREFFHQPESERVKHYSADPTKTTRLSTSF-NVGAD 130

Query: 129 QIHCWRDALIFNCHPLEKYVHFWPQKPPKYREVVGAYCIAMRKLVLEILELMSEGLGLGK 188
           ++  WRD L  +C P+E ++  WP  P  +REV   Y  ++R LVL +LE +SE LGL  
Sbjct: 131 KVLNWRDFLRLHCFPIEDFIEEWPSSPISFREVTAEYATSVRALVLRLLEAISESLGLES 190

Query: 189 GYFGGEMSENPL-LLVNHYPPCPNPSLTLGLSQHCDPSLITILFQD-VNGLQVLKDGQWI 248
            +    + ++   +  N+YPPCP P LT GL  H DP++IT+L QD V+GLQV KD +W+
Sbjct: 191 DHISNILGKHAQHMAFNYYPPCPEPELTYGLPGHKDPTVITVLLQDQVSGLQVFKDDKWV 250

Query: 249 GVQPIDNAFVINIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKC 308
            V PI N F++NIG  +Q+I+N K ++  HRAV N++  R S+    +P  +A++ PA  
Sbjct: 251 AVSPIPNTFIVNIGDQMQVISNDKYKSVLHRAVVNTENERLSIPTFYFPSTDAVIGPAHE 310

Query: 309 MINEANP-PRYRSLNFKDFQRNYLPRAVDTKAVM 334
           ++NE +    YR+  F ++   +  R++ T + +
Sbjct: 311 LVNEQDSLAIYRTYPFVEYWDKFWNRSLATASCL 341

BLAST of Chy1G015200 vs. TAIR 10
Match: AT2G44800.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 197.2 bits (500), Expect = 2.1e-50
Identity = 111/317 (35.02%), Postives = 171/317 (53.94%), Query Frame = 0

Query: 15  VESVPESYVYPPEKRP--GNIVVPMAKAIPVIDLSIRD----RTLLVRKILDASKEFGFF 74
           V  VP+ YV PP +RP  G+ +      +PVIDLS+      R+L + +I  A KEFGFF
Sbjct: 24  VPQVPDRYVLPPSQRPALGSSLGTSETTLPVIDLSLLHQPFLRSLAIHEISMACKEFGFF 83

Query: 75  QIINHGVSKKVSEETMRIFKEFHSMSGPEKAKECSKDPNRSCRVYTSSENYTKEQIHCWR 134
           Q+INHG+   V  + +    +F  +   EK    S + +   R Y +S N++ +++H WR
Sbjct: 84  QVINHGIPSSVVNDALDAATQFFDLPVEEKMLLVSANVHEPVR-YGTSLNHSTDRVHYWR 143

Query: 135 DALIFNCHPLEKYVHFWPQKPPKYREVVGAYCIAMRKLVLEILELMSEGLGLGKGYFGGE 194
           D +    HPL K++  WP  PP Y++ VG Y  A   L  +++E +SE LGL K Y   E
Sbjct: 144 DFIKHYSHPLSKWIDMWPSNPPCYKDKVGKYAEATHLLHKQLIEAISESLGLEKNYLQEE 203

Query: 195 MSE-NPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLK-DGQWIGVQPID 254
           + E + ++ VN YP CP P + LG+  H D S +TIL Q   GLQ++  +  W+ V  I+
Sbjct: 204 IEEGSQVMAVNCYPACPEPEMALGMPPHSDFSSLTILLQSSKGLQIMDCNKNWVCVPYIE 263

Query: 255 NAFVINIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKCMINEAN 314
            A ++ +G  +++++NG  ++  HR   N +  R S   L        + PA  ++N  N
Sbjct: 264 GALIVQLGDQVEVMSNGIYKSVIHRVTVNKEVKRLSFASLHSLPLHKKISPAPKLVNPNN 323

Query: 315 PPRYRSLNFKDFQRNYL 324
            P Y   +F DF  NY+
Sbjct: 324 APAYGEFSFNDF-LNYI 338

BLAST of Chy1G015200 vs. TAIR 10
Match: AT3G19000.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 189.9 bits (481), Expect = 3.4e-48
Identity = 118/327 (36.09%), Postives = 171/327 (52.29%), Query Frame = 0

Query: 20  ESYVYPPEKRPGNIVVP-----MAKAIPVIDLSI-----RDRTLLVRKILDASKEFGFFQ 79
           E+++  PE RP   +        +  IP IDLS       D+T + ++I +A K +GFFQ
Sbjct: 6   EAFIQAPEHRPNTHLTNSGDFIFSDEIPTIDLSSLEDTHHDKTAIAKEIAEACKRWGFFQ 65

Query: 80  IINHGVSKKVSEETMRIFKEFHSMSGPEKAKECSKDPNRSCRVYTSSENYTKEQIHCWR- 139
           +INHG+   +     +   EF +++  EK K    + N    +    E +TK  +  W+ 
Sbjct: 66  VINHGLPSALRHRVEKTAAEFFNLTTEEKRKVKRDEVN---PMGYHDEEHTK-NVRDWKE 125

Query: 140 -------DALIFNCHP------LEKYVHFWPQKPPKYREVVGAYCIAMRKLVLEILELMS 199
                  D+ I    P      L K  + WPQ P  +REV   Y   + KL   +LEL+S
Sbjct: 126 IFDFFLQDSTIVPASPEPEDTELRKLTNQWPQNPSHFREVCQEYAREVEKLAFRLLELVS 185

Query: 200 EGLGL-GKGYFGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQD-VNGLQV 259
             LGL G    G    +   L  NHYPPCPNP L LG+ +H D   +T+L QD V GLQV
Sbjct: 186 ISLGLPGDRLTGFFNEQTSFLRFNHYPPCPNPELALGVGRHKDGGALTVLAQDSVGGLQV 245

Query: 260 LK--DGQWIGVQPIDNAFVINIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKD 319
            +  DGQWI V+PI +A +IN+G  +Q+ TN +  +AEHR V N+   R S+ +  +P  
Sbjct: 246 SRRSDGQWIPVKPISDALIINMGNCIQVWTNDEYWSAEHRVVVNTSKERFSIPFFFFPSH 305