Chy1G010120 (gene) Cucumber (hystrix) v1

Overview
NameChy1G010120
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionNitric oxide synthase-interacting protein-like
LocationchrH01: 10675987 .. 10676225 (-)
RNA-Seq ExpressionChy1G010120
SyntenyChy1G010120
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCTCAGAGACACTCAAAAAATAATAATGATCTAGCCTTCTTCACCTATGATGAGAAGCGGAAGCTCGGCTATGGAACACAAAAAGAGAGACTTGGAAAGGACTCGATCAAGCCCTTCGATGTCTGTTGCCTATGCTTAAAACCCTTCATCGACCCCATGTGTTGTCAAAAGGGTCACACGTTTTGTAAAGAATGCATTCTTGAGTGCCTTTTGTCTCATAAGAAAGATAATCAAAG

mRNA sequence

ATGCCTCAGAGACACTCAAAAAATAATAATGATCTAGCCTTCTTCACCTATGATGAGAAGCGGAAGCTCGGCTATGGAACACAAAAAGAGAGACTTGGAAAGGACTCGATCAAGCCCTTCGATGTCTGTTGCCTATGCTTAAAACCCTTCATCGACCCCATGTGTTGTCAAAAGGGTCACACGTTTTGTAAAGAATGCATTCTTGAGTGCCTTTTGTCTCATAAGAAAGATAATCAAAG

Coding sequence (CDS)

ATGCCTCAGAGACACTCAAAAAATAATAATGATCTAGCCTTCTTCACCTATGATGAGAAGCGGAAGCTCGGCTATGGAACACAAAAAGAGAGACTTGGAAAGGACTCGATCAAGCCCTTCGATGTCTGTTGCCTATGCTTAAAACCCTTCATCGACCCCATGTGTTGTCAAAAGGGTCACACGTTTTGTAAAGAATGCATTCTTGAGTGCCTTTTGTCTCATAAGAAAGATAATCAAAG

Protein sequence

MPQRHSKNNNDLAFFTYDEKRKLGYGTQKERLGKDSIKPFDVCCLCLKPFIDPMCCQKGHTFCKECILECLLSHKKDNQX
Homology
BLAST of Chy1G010120 vs. ExPASy Swiss-Prot
Match: Q9SY88 (E3 ubiquitin-protein ligase CSU1 OS=Arabidopsis thaliana OX=3702 GN=CSU1 PE=1 SV=1)

HSP 1 Score: 154.1 bits (388), Expect = 6.8e-37
Identity = 66/79 (83.54%), Postives = 72/79 (91.14%), Query Frame = 0

Query: 1  MPQRHSKNNNDLAFFTYDEKRKLGYGTQKERLGKDSIKPFDVCCLCLKPFIDPMCCQKGH 60
          MPQRHSKNNNDLA+FTYDEK+KLGYGTQ+ERLG+DSIKPFD C LCLKPFIDPMCC KGH
Sbjct: 1  MPQRHSKNNNDLAYFTYDEKKKLGYGTQRERLGRDSIKPFDACSLCLKPFIDPMCCHKGH 60

Query: 61 TFCKECILECLLSHKKDNQ 80
           FC+ECILEC L+ KKD Q
Sbjct: 61 VFCRECILECFLAQKKDIQ 79

BLAST of Chy1G010120 vs. ExPASy Swiss-Prot
Match: Q55DU4 (Nitric oxide synthase-interacting protein homolog OS=Dictyostelium discoideum OX=44689 GN=nosip PE=2 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 9.6e-15
Identity = 40/74 (54.05%), Postives = 50/74 (67.57%), Query Frame = 0

Query: 4  RHSKNN-NDLAFFTYDEKRKLGYGTQKERLGKDSIKPFDVCCLCLKPFIDPMCCQKGHTF 63
          RHSK++ ++   F+  E++ L YGT  ERLGKDSIK FD C LCL   I P+ C KGH +
Sbjct: 3  RHSKHSCSNSGKFSNYERQLLKYGTTTERLGKDSIKNFDSCSLCLNDVIMPVICNKGHLY 62

Query: 64 CKECILECLLSHKK 77
          CKECIL  L+  KK
Sbjct: 63 CKECILTSLIDQKK 76

BLAST of Chy1G010120 vs. ExPASy Swiss-Prot
Match: Q6P829 (Nitric oxide synthase-interacting protein OS=Xenopus tropicalis OX=8364 GN=nosip PE=2 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 2.0e-12
Identity = 38/78 (48.72%), Postives = 48/78 (61.54%), Query Frame = 0

Query: 4  RHSKNNNDLAFFTYDEKRK----LGYGTQKERLGKDSIKPFDVCCLCLKPFIDPMCCQKG 63
          RH KN    A +TY EK+K     GYGTQ  RL KD++K FD CCL L+P  DP+    G
Sbjct: 3  RHGKNCTAGAVYTYYEKKKDTAASGYGTQTVRLSKDAVKDFDCCCLSLQPCKDPVVTSDG 62

Query: 64 HTFCKECILECLLSHKKD 78
          + + KE ILE +L  KK+
Sbjct: 63 YIYEKEAILEYILHQKKE 80

BLAST of Chy1G010120 vs. ExPASy Swiss-Prot
Match: Q6NUH3 (Nitric oxide synthase-interacting protein OS=Xenopus laevis OX=8355 GN=nosip PE=2 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 2.6e-12
Identity = 38/78 (48.72%), Postives = 48/78 (61.54%), Query Frame = 0

Query: 4  RHSKNNNDLAFFTYDEKRK----LGYGTQKERLGKDSIKPFDVCCLCLKPFIDPMCCQKG 63
          RH KN    A +TY EK+K     GYGTQ  RL KD++K FD CCL L+P  DP+    G
Sbjct: 3  RHGKNCTAGAVYTYYEKKKDTVASGYGTQTVRLSKDAVKDFDCCCLSLQPCKDPVVTSDG 62

Query: 64 HTFCKECILECLLSHKKD 78
          + + KE ILE +L  KK+
Sbjct: 63 YIYEKESILEYILHQKKE 80

BLAST of Chy1G010120 vs. ExPASy Swiss-Prot
Match: Q5U3S7 (Nitric oxide synthase-interacting protein OS=Danio rerio OX=7955 GN=nosip PE=2 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 5.8e-12
Identity = 38/78 (48.72%), Postives = 48/78 (61.54%), Query Frame = 0

Query: 4  RHSKNNNDLAFFTYDEKRK----LGYGTQKERLGKDSIKPFDVCCLCLKPFIDPMCCQKG 63
          RH KN    A +TY EKRK     GYGTQ  RLGKD+IK FD C L L+P  DP+  + G
Sbjct: 3  RHGKNCTAGAVYTYHEKRKDTAASGYGTQSVRLGKDAIKDFDCCSLSLQPCRDPVLTEDG 62

Query: 64 HTFCKECILECLLSHKKD 78
          + + KE IL+ +L  K +
Sbjct: 63 YLYEKEAILQYILHQKTE 80

BLAST of Chy1G010120 vs. ExPASy TrEMBL
Match: A0A1S3BI71 (nitric oxide synthase-interacting protein-like OS=Cucumis melo OX=3656 GN=LOC103490169 PE=3 SV=1)

HSP 1 Score: 175.3 bits (443), Expect = 1.1e-40
Identity = 78/79 (98.73%), Postives = 78/79 (98.73%), Query Frame = 0

Query: 1  MPQRHSKNNNDLAFFTYDEKRKLGYGTQKERLGKDSIKPFDVCCLCLKPFIDPMCCQKGH 60
          MPQRHSKNNNDLAFFTYDEKRKLGYGTQKERLGKDSIKPFDVCCLCLKPFIDPMCCQKGH
Sbjct: 1  MPQRHSKNNNDLAFFTYDEKRKLGYGTQKERLGKDSIKPFDVCCLCLKPFIDPMCCQKGH 60

Query: 61 TFCKECILECLLSHKKDNQ 80
          TFCKECILECLLS KKDNQ
Sbjct: 61 TFCKECILECLLSQKKDNQ 79

BLAST of Chy1G010120 vs. ExPASy TrEMBL
Match: A0A5D3DI86 (Nitric oxide synthase-interacting protein-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold142G002050 PE=3 SV=1)

HSP 1 Score: 175.3 bits (443), Expect = 1.1e-40
Identity = 78/79 (98.73%), Postives = 78/79 (98.73%), Query Frame = 0

Query: 1  MPQRHSKNNNDLAFFTYDEKRKLGYGTQKERLGKDSIKPFDVCCLCLKPFIDPMCCQKGH 60
          MPQRHSKNNNDLAFFTYDEKRKLGYGTQKERLGKDSIKPFDVCCLCLKPFIDPMCCQKGH
Sbjct: 1  MPQRHSKNNNDLAFFTYDEKRKLGYGTQKERLGKDSIKPFDVCCLCLKPFIDPMCCQKGH 60

Query: 61 TFCKECILECLLSHKKDNQ 80
          TFCKECILECLLS KKDNQ
Sbjct: 61 TFCKECILECLLSQKKDNQ 79

BLAST of Chy1G010120 vs. ExPASy TrEMBL
Match: A0A0A0K5A3 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G195280 PE=3 SV=1)

HSP 1 Score: 175.3 bits (443), Expect = 1.1e-40
Identity = 78/79 (98.73%), Postives = 78/79 (98.73%), Query Frame = 0

Query: 1  MPQRHSKNNNDLAFFTYDEKRKLGYGTQKERLGKDSIKPFDVCCLCLKPFIDPMCCQKGH 60
          MPQRHSKNNNDLAFFTYDEKRKLGYGTQKERLGKDSIKPFDVCCLCLKPFIDPMCCQKGH
Sbjct: 1  MPQRHSKNNNDLAFFTYDEKRKLGYGTQKERLGKDSIKPFDVCCLCLKPFIDPMCCQKGH 60

Query: 61 TFCKECILECLLSHKKDNQ 80
          TFCKECILECLLS KKDNQ
Sbjct: 61 TFCKECILECLLSQKKDNQ 79

BLAST of Chy1G010120 vs. ExPASy TrEMBL
Match: A0A5A7TEE0 (Nitric oxide synthase-interacting protein-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G001520 PE=3 SV=1)

HSP 1 Score: 172.9 bits (437), Expect = 5.2e-40
Identity = 76/79 (96.20%), Postives = 78/79 (98.73%), Query Frame = 0

Query: 1  MPQRHSKNNNDLAFFTYDEKRKLGYGTQKERLGKDSIKPFDVCCLCLKPFIDPMCCQKGH 60
          MPQRHSKNNNDLAFFTYDEKRKLGYGTQKERLGKDSIKPFDVCCLCLKPFIDPMCCQKGH
Sbjct: 1  MPQRHSKNNNDLAFFTYDEKRKLGYGTQKERLGKDSIKPFDVCCLCLKPFIDPMCCQKGH 60

Query: 61 TFCKECILECLLSHKKDNQ 80
          TFCK+CILECLL+ KKDNQ
Sbjct: 61 TFCKQCILECLLAQKKDNQ 79

BLAST of Chy1G010120 vs. ExPASy TrEMBL
Match: A0A0A0KF19 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G087940 PE=3 SV=1)

HSP 1 Score: 172.9 bits (437), Expect = 5.2e-40
Identity = 76/79 (96.20%), Postives = 78/79 (98.73%), Query Frame = 0

Query: 1  MPQRHSKNNNDLAFFTYDEKRKLGYGTQKERLGKDSIKPFDVCCLCLKPFIDPMCCQKGH 60
          MPQRHSKNNNDLAFFTYDEKRKLGYGTQKERLGKDSIKPFDVCCLCLKPFIDPMCCQKGH
Sbjct: 1  MPQRHSKNNNDLAFFTYDEKRKLGYGTQKERLGKDSIKPFDVCCLCLKPFIDPMCCQKGH 60

Query: 61 TFCKECILECLLSHKKDNQ 80
          TFCK+CILECLL+ KKDNQ
Sbjct: 61 TFCKQCILECLLAQKKDNQ 79

BLAST of Chy1G010120 vs. NCBI nr
Match: XP_008447780.1 (PREDICTED: nitric oxide synthase-interacting protein-like [Cucumis melo] >XP_008447781.1 PREDICTED: nitric oxide synthase-interacting protein-like [Cucumis melo] >TYK23178.1 nitric oxide synthase-interacting protein-like [Cucumis melo var. makuwa])

HSP 1 Score: 172 bits (435), Expect = 3.95e-51
Identity = 78/79 (98.73%), Postives = 78/79 (98.73%), Query Frame = 0

Query: 1  MPQRHSKNNNDLAFFTYDEKRKLGYGTQKERLGKDSIKPFDVCCLCLKPFIDPMCCQKGH 60
          MPQRHSKNNNDLAFFTYDEKRKLGYGTQKERLGKDSIKPFDVCCLCLKPFIDPMCCQKGH
Sbjct: 1  MPQRHSKNNNDLAFFTYDEKRKLGYGTQKERLGKDSIKPFDVCCLCLKPFIDPMCCQKGH 60

Query: 61 TFCKECILECLLSHKKDNQ 79
          TFCKECILECLLS KKDNQ
Sbjct: 61 TFCKECILECLLSQKKDNQ 79

BLAST of Chy1G010120 vs. NCBI nr
Match: XP_004139773.1 (E3 ubiquitin-protein ligase CSU1 [Cucumis sativus] >KGN44119.1 hypothetical protein Csa_016343 [Cucumis sativus])

HSP 1 Score: 172 bits (435), Expect = 3.95e-51
Identity = 78/79 (98.73%), Postives = 78/79 (98.73%), Query Frame = 0

Query: 1  MPQRHSKNNNDLAFFTYDEKRKLGYGTQKERLGKDSIKPFDVCCLCLKPFIDPMCCQKGH 60
          MPQRHSKNNNDLAFFTYDEKRKLGYGTQKERLGKDSIKPFDVCCLCLKPFIDPMCCQKGH
Sbjct: 1  MPQRHSKNNNDLAFFTYDEKRKLGYGTQKERLGKDSIKPFDVCCLCLKPFIDPMCCQKGH 60

Query: 61 TFCKECILECLLSHKKDNQ 79
          TFCKECILECLLS KKDNQ
Sbjct: 61 TFCKECILECLLSQKKDNQ 79

BLAST of Chy1G010120 vs. NCBI nr
Match: XP_038897586.1 (E3 ubiquitin-protein ligase CSU1-like [Benincasa hispida] >XP_038897587.1 E3 ubiquitin-protein ligase CSU1-like [Benincasa hispida])

HSP 1 Score: 171 bits (432), Expect = 1.12e-50
Identity = 77/79 (97.47%), Postives = 78/79 (98.73%), Query Frame = 0

Query: 1  MPQRHSKNNNDLAFFTYDEKRKLGYGTQKERLGKDSIKPFDVCCLCLKPFIDPMCCQKGH 60
          MPQRHSKNNNDLAFFTYDEKRKLGYGTQKERLGKDSIKPFDVCCLCLKPFIDPMCCQKGH
Sbjct: 1  MPQRHSKNNNDLAFFTYDEKRKLGYGTQKERLGKDSIKPFDVCCLCLKPFIDPMCCQKGH 60

Query: 61 TFCKECILECLLSHKKDNQ 79
          TFCKECILECLL+ KKDNQ
Sbjct: 61 TFCKECILECLLAQKKDNQ 79

BLAST of Chy1G010120 vs. NCBI nr
Match: PKI35844.1 (hypothetical protein CRG98_043753 [Punica granatum])

HSP 1 Score: 162 bits (411), Expect = 2.81e-50
Identity = 73/79 (92.41%), Postives = 75/79 (94.94%), Query Frame = 0

Query: 1  MPQRHSKNNNDLAFFTYDEKRKLGYGTQKERLGKDSIKPFDVCCLCLKPFIDPMCCQKGH 60
          MPQRHSKNNNDLAFFTYDEKRKLGYGTQ+ERLGKDSIKPFD CCLCLKPFIDPMCCQKGH
Sbjct: 1  MPQRHSKNNNDLAFFTYDEKRKLGYGTQRERLGKDSIKPFDACCLCLKPFIDPMCCQKGH 60

Query: 61 TFCKECILECLLSHKKDNQ 79
           FCKECILECLL+ KKD Q
Sbjct: 61 VFCKECILECLLAQKKDIQ 79

BLAST of Chy1G010120 vs. NCBI nr
Match: KDO75673.1 (hypothetical protein CISIN_1g0220292mg, partial [Citrus sinensis])

HSP 1 Score: 162 bits (410), Expect = 2.96e-50
Identity = 73/79 (92.41%), Postives = 75/79 (94.94%), Query Frame = 0

Query: 1  MPQRHSKNNNDLAFFTYDEKRKLGYGTQKERLGKDSIKPFDVCCLCLKPFIDPMCCQKGH 60
          MPQRHSKNNNDLAFFTYDEKRKLGYGTQKERLGKDSIKPFD CCLCLKPFIDP+CCQKGH
Sbjct: 1  MPQRHSKNNNDLAFFTYDEKRKLGYGTQKERLGKDSIKPFDACCLCLKPFIDPLCCQKGH 60

Query: 61 TFCKECILECLLSHKKDNQ 79
           +CKECILECLLS KKD Q
Sbjct: 61 IYCKECILECLLSQKKDIQ 79

BLAST of Chy1G010120 vs. TAIR 10
Match: AT1G61620.1 (phosphoinositide binding )

HSP 1 Score: 154.1 bits (388), Expect = 4.8e-38
Identity = 66/79 (83.54%), Postives = 72/79 (91.14%), Query Frame = 0

Query: 1  MPQRHSKNNNDLAFFTYDEKRKLGYGTQKERLGKDSIKPFDVCCLCLKPFIDPMCCQKGH 60
          MPQRHSKNNNDLA+FTYDEK+KLGYGTQ+ERLG+DSIKPFD C LCLKPFIDPMCC KGH
Sbjct: 1  MPQRHSKNNNDLAYFTYDEKKKLGYGTQRERLGRDSIKPFDACSLCLKPFIDPMCCHKGH 60

Query: 61 TFCKECILECLLSHKKDNQ 80
           FC+ECILEC L+ KKD Q
Sbjct: 61 VFCRECILECFLAQKKDIQ 79

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SY886.8e-3783.54E3 ubiquitin-protein ligase CSU1 OS=Arabidopsis thaliana OX=3702 GN=CSU1 PE=1 SV... [more]
Q55DU49.6e-1554.05Nitric oxide synthase-interacting protein homolog OS=Dictyostelium discoideum OX... [more]
Q6P8292.0e-1248.72Nitric oxide synthase-interacting protein OS=Xenopus tropicalis OX=8364 GN=nosip... [more]
Q6NUH32.6e-1248.72Nitric oxide synthase-interacting protein OS=Xenopus laevis OX=8355 GN=nosip PE=... [more]
Q5U3S75.8e-1248.72Nitric oxide synthase-interacting protein OS=Danio rerio OX=7955 GN=nosip PE=2 S... [more]
Match NameE-valueIdentityDescription
A0A1S3BI711.1e-4098.73nitric oxide synthase-interacting protein-like OS=Cucumis melo OX=3656 GN=LOC103... [more]
A0A5D3DI861.1e-4098.73Nitric oxide synthase-interacting protein-like OS=Cucumis melo var. makuwa OX=11... [more]
A0A0A0K5A31.1e-4098.73RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G195280 P... [more]
A0A5A7TEE05.2e-4096.20Nitric oxide synthase-interacting protein-like OS=Cucumis melo var. makuwa OX=11... [more]
A0A0A0KF195.2e-4096.20RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G087940 P... [more]
Match NameE-valueIdentityDescription
XP_008447780.13.95e-5198.73PREDICTED: nitric oxide synthase-interacting protein-like [Cucumis melo] >XP_008... [more]
XP_004139773.13.95e-5198.73E3 ubiquitin-protein ligase CSU1 [Cucumis sativus] >KGN44119.1 hypothetical prot... [more]
XP_038897586.11.12e-5097.47E3 ubiquitin-protein ligase CSU1-like [Benincasa hispida] >XP_038897587.1 E3 ubi... [more]
PKI35844.12.81e-5092.41hypothetical protein CRG98_043753 [Punica granatum][more]
KDO75673.12.96e-5092.41hypothetical protein CISIN_1g0220292mg, partial [Citrus sinensis][more]
Match NameE-valueIdentityDescription
AT1G61620.14.8e-3883.54phosphoinositide binding [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR031790Nitric oxide synthase-interacting protein, zinc-fingerPFAMPF15906zf-NOSIPcoord: 5..75
e-value: 2.1E-16
score: 59.8
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 19..79
e-value: 1.6E-9
score: 39.5
IPR016818Nitric oxide synthase-interacting proteinPANTHERPTHR13063ENOS INTERACTING PROTEINcoord: 1..78
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 24..77

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy1G010120.1Chy1G010120.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0005634 nucleus
molecular_function GO:0046872 metal ion binding
molecular_function GO:0061630 ubiquitin protein ligase activity