Chy1G008030 (gene) Cucumber (hystrix) v1

BLAST of Chy1G008030 vs. ExPASy Swiss-Prot
Match: Q9SXA1 (Phosphatidylinositol 4-kinase alpha 1 OS=Arabidopsis thaliana OX=3702 GN=PI4KA1 PE=1 SV=2)

HSP 1 Score: 2989.5 bits (7749), Expect = 0.0e+00
Identity = 1521/2050 (74.20%), Postives = 1742/2050 (84.98%), Query Frame = 0

Query: 1    MDALIELCDLIAQNPALFSEKLSWICSRCPPSEAILAGSPAISRSQLNAVLAVARLLSKC 60
            M+AL ELCD+IA+NP  FSEKL+WIC RCP +E +LA SP +SRS LNAVLAVAR++SK 
Sbjct: 1    MEALTELCDIIAKNPKQFSEKLAWICGRCPQTEWLLAESPRVSRSHLNAVLAVARIISKN 60

Query: 61   PDSVGLRPKSVVLEFLRSIPLSFSLSFWPQSYGNDAIASFFNEFLNYTSKACALSTDFAT 120
            P+S+  R KSVV EFL +IP SF  SFWP S+ +  I+SF+ +FL+Y S A  LS +F T
Sbjct: 61   PESIDNRAKSVVNEFLSAIPASFRRSFWPHSFPSQLISSFYCDFLSYLSCAADLSPEFGT 120

Query: 121  EVSGFSSEVVLSAI----NDCSEGSAISRAFLVALSKSFLPIIPSDADKLVSCILDRFLI 180
            EV+ F+ EVV++AI     D     AIS+AFLVALS+ F  I+ SD DKL++ +LD+F++
Sbjct: 121  EVARFTGEVVIAAIAPSSGDSDGDPAISKAFLVALSQHFPSILQSDGDKLITMLLDQFVL 180

Query: 181  SEAAPGTPREHNQANS-----EPSSSQSSPLSVSHQPSNGG--LSPGNENGQVSGSLSSG 240
            +  AP +P+E  Q NS     + SSSQ SP+S +  PS      SPG+E      +LSS 
Sbjct: 181  NR-APASPKEQRQQNSANSETDTSSSQGSPISTNRYPSGKTEMASPGDEVASHGSNLSS- 240

Query: 241  ASRITDDATSASSRGSGMMNGNSILWKSGLEQLGMNLGFSEGGGVA--FVRQQVALFVDE 300
                        S  S +MNG SI+WKSG++QL  + GFSEG G A    RQQVA F DE
Sbjct: 241  -----------KSSSSVVMNGGSIVWKSGVDQL--SFGFSEGSGGANPVFRQQVASFEDE 300

Query: 301  SIENLEKQEIAFKLMTHILDNSSFDARLWEQMRALAKKQLQTLPTFLKIKKRDWSEQGSV 360
            SIE+LEKQEIAF+L+THILD    D++L +Q+R +AK+QLQ++  FLK +KRDW+EQG V
Sbjct: 301  SIESLEKQEIAFRLITHILDKVKIDSKLQDQVRFIAKRQLQSMSAFLKSRKRDWNEQGQV 360

Query: 361  LKARINTKLLVYQAAARLKMKTVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLR 420
            LK R+N KL VYQAAA++K+K++ SL+ DGK +K+L+ ET ALL+DAA+ACL SVWRK++
Sbjct: 361  LKTRVNAKLSVYQAAAKMKIKSLVSLETDGKTSKRLVLETLALLLDAADACLTSVWRKMK 420

Query: 421  ICEELFSSLLTGLAQIAVARGGQPLRVLLIRLKPLVLTVCVQADTWGTNQGAMFESVLTT 480
             CEELF SLL+G+A+IAVARGGQPLRVLLIRLKPLVL VC   D     QGAM ES+  T
Sbjct: 421  ACEELFDSLLSGIAKIAVARGGQPLRVLLIRLKPLVLAVCALPD-----QGAMLESIFKT 480

Query: 481  CCEIIESCWTKDRAPVDTFIMGLATSIRDRNDSEEQDDKEKQGVPMQLNVIRLLAKMTVA 540
             C IIES W KDRAPVD FIMGLA+SIR+RND EEQ D+EKQ   +QLNVIRLLA + VA
Sbjct: 481  SCVIIESAWAKDRAPVDNFIMGLASSIRERNDYEEQVDREKQVPAVQLNVIRLLADLNVA 540

Query: 541  VNKSEIVDMILPLFIESLEEGDASTPGLLRLQLLDAVSRMATLGFEKSYRETIVLMTRSY 600
            V K E+ DMILPLFIESLEEGDASTP  LRLQLLDAVSR+ATLGF+KSYRET+VLMTRSY
Sbjct: 541  VKKPEVADMILPLFIESLEEGDASTPSFLRLQLLDAVSRIATLGFDKSYRETVVLMTRSY 600

Query: 601  LSKLSSIGSSESRTVAPEATTERVEILPAGFLHIASGLKSAKLRLEYRHRLLSLCSDVGL 660
            LSKLSS+GS ES+T APEATTERVE LPAGFL IASGL   KLR +YRHRLLSLCSDVGL
Sbjct: 601  LSKLSSVGSVESKTSAPEATTERVETLPAGFLTIASGLMDTKLRSDYRHRLLSLCSDVGL 660

Query: 661  AAESKSGRSGANFLGPLLPAVAEICSDFDPTMDIEPSLLKLFRNLWFYIALFGLAPPIQN 720
            AAESKSG SG +FLGPLLPAVAEICSDFDPTMD+EPSLLKLFRNLWFYIALFGLAPPI  
Sbjct: 661  AAESKSGGSGVDFLGPLLPAVAEICSDFDPTMDVEPSLLKLFRNLWFYIALFGLAPPIVK 720

Query: 721  SHL-QTKSVSTMLNSVGS-TAIALQAVSGPYLWNTQWSSAVQLIARGTPPLVVSSVKWLE 780
            +     KS S  +NSVGS +A ALQAV GPY+W+ QW+ AVQ IA+GTPPLVVSSVKWLE
Sbjct: 721  TPTPPLKSTSNSVNSVGSMSATALQAVGGPYMWDNQWALAVQRIAQGTPPLVVSSVKWLE 780

Query: 781  DELELNALHNPGSRRGSGNEKAALAQRAALSSALGGRVDVAAMSTISGVKATYLLAVSFL 840
            DELELNALHNPGSRRG+GNEK A  QR ALS+ALGGRVDVAAM+TISGVKATYLLAV+FL
Sbjct: 781  DELELNALHNPGSRRGNGNEKVASTQRLALSTALGGRVDVAAMNTISGVKATYLLAVAFL 840

Query: 841  EIIRFSSNGGILNGGSNVNASRSAFCCVFEYLKTPNLLPAVSQCLTAIVHRAFETAVLWL 900
            EIIRF SNGGILNG S+V+ASRSAF CVFEYLKTPNL PAVSQCLTAIVHRAFETAV WL
Sbjct: 841  EIIRFISNGGILNGESSVSASRSAFSCVFEYLKTPNLTPAVSQCLTAIVHRAFETAVSWL 900

Query: 901  EDRISDTGNEAEVRDSTLFAHTCYLIKSMSQRDEHVRDIAVNLLTQLRDKFPQVMWNSSC 960
            EDRIS TG +A  R+ T +AH C+LIKSMSQRDEHVRDI+VNLLTQLRDKFPQV+W+SSC
Sbjct: 901  EDRISLTGKDARNRELTTYAHACFLIKSMSQRDEHVRDISVNLLTQLRDKFPQVLWHSSC 960

Query: 961  LDSLLFSMHNDAPSTVVTDPAWVVTVRSLYQRVVREWIVKSLSYAPCTCQGLLQEKLCKA 1020
            LDSLLFS+H++ PSTVV DPAW   VRSLYQ+VVREWI+ SLSYAPCT QGLLQ+KLCKA
Sbjct: 961  LDSLLFSVHDNTPSTVVNDPAWTAAVRSLYQKVVREWIIISLSYAPCTSQGLLQDKLCKA 1020

Query: 1021 NTWQRAQHTPDVICLLSEIRIGTSKNEHWTGIQTANIPAVIAAAAAASGADLKLTEAFNL 1080
            NTWQRAQ T DV+ LLSEI+IGT KNE W+GI+TANIPAV+AAAAAASGA+LK++EAFNL
Sbjct: 1021 NTWQRAQTTTDVVSLLSEIKIGTGKNELWSGIRTANIPAVMAAAAAASGANLKVSEAFNL 1080

Query: 1081 EVLSTGMVSATVKCNHAGEIAGMRRLYNSIGGFQTGVAGLGFGQGLQRLITGALPQQPQN 1140
            EVL TG+VSATVKCNHAGEIAGMRRLYNSIGGFQ+G    GFG GLQRLI+GA  Q PQ 
Sbjct: 1081 EVLGTGVVSATVKCNHAGEIAGMRRLYNSIGGFQSGSTPSGFGGGLQRLISGAFSQAPQP 1140

Query: 1141 EDDSFNGILIMKFVQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLASESKTNIEG 1200
            EDDSFN +LI +FV+ LQQFV+ AEKG  ++K +FRETCSQATALLLSNL  ESKTN+EG
Sbjct: 1141 EDDSFNEMLIARFVRLLQQFVNTAEKGGEVEKSQFRETCSQATALLLSNLGGESKTNVEG 1200

Query: 1201 FAQLIRLLCWCPAYISTPDAIETGVFVWTWLVSAAPELGSFVLAELVDAWLWTVDTKRGL 1260
            F+QL+RLLCWCPAYISTPDA+ETG+F+WTWLVSAAP+L S VLAELVDAW+WT+DTKRGL
Sbjct: 1201 FSQLLRLLCWCPAYISTPDAMETGIFIWTWLVSAAPQLVSLVLAELVDAWIWTIDTKRGL 1260

Query: 1261 FASDVKYSGPAAMLRPHLSPGEPEMQPEIDPVEQIIAHKIWLGFFIDRFEVVRHNSVEQL 1320
            FASDV+YSGPAA LRPHLSPGEPE  PE DPV+QI+AH++WLGF IDRFEVVRHNS EQL
Sbjct: 1261 FASDVRYSGPAAKLRPHLSPGEPEDPPESDPVDQIVAHRLWLGFLIDRFEVVRHNSAEQL 1320

Query: 1321 LLFGRLLQGSTKPPWNFSRHPAATGSFFTLMLLGLKFCSCQAQGNLQNFKTGLELLEDRI 1380
            LL GR+LQ ST   W F+RHPAA G+FF+LMLLGLKFCSCQ QGN+Q F++GL+LLEDRI
Sbjct: 1321 LLLGRMLQRSTDLEWCFTRHPAAAGTFFSLMLLGLKFCSCQTQGNMQKFRSGLQLLEDRI 1380

Query: 1381 YRASLGWFAHEPEWYDVKHVNFAQSEAQSVSIFLHYLSSERGNSLHSDAKMRGRENGISL 1440
            YR SLGWFAH+PEWYDV   NF  SEA SVS+F+H+LS+E   S  SD+K + RE+G +L
Sbjct: 1381 YRTSLGWFAHQPEWYDVNIPNFCHSEALSVSVFVHFLSNELSESSQSDSKGKPRESG-NL 1440

Query: 1441 IDLNDHYHPVWGHLENYAVGREKRRQLLLMLCQHEADRLEVWAQP-NIKESTPSRPKLTA 1500
            ID+ D YHPVWG ++NY +G+EKR+QLLLMLCQHEADRL+VWAQP + K+S  SR K+++
Sbjct: 1441 IDVTDQYHPVWGEMDNYTLGKEKRKQLLLMLCQHEADRLDVWAQPISSKDSPYSRLKISS 1500

Query: 1501 EKWIEHARTAFSVDPRIAFSMVSRFPTNAFLRVEMNQLVQLHILDIRSIPEALPYFVTPK 1560
            EKW E+A+TAFSVDPRIA S+ SRFP NA ++ E+ QLVQ +I+D+R+IPEALPYFVTPK
Sbjct: 1501 EKWTEYAKTAFSVDPRIALSVASRFPANASVKSEVTQLVQTNIVDLRTIPEALPYFVTPK 1560

Query: 1561 AVDENSELLRQLPHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQL 1620
             V+ENS LL+QLPHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPE+VTFFMPQL
Sbjct: 1561 NVEENSVLLQQLPHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPERVTFFMPQL 1620

Query: 1621 VQALRYDEGRLVEGYLLRAAKRSDIFAHILIWHLQGETSLPDSGKDVNSGKNGSFLALLP 1680
            VQ+LRYD+GRLVEGYLLRA +RSDIFAHILIWHLQGE  + ++ KD +  KN +F  +LP
Sbjct: 1621 VQSLRYDDGRLVEGYLLRATQRSDIFAHILIWHLQGE-DVQETPKDGSIDKNAAFQEILP 1680

Query: 1681 VVRQRIIDGFTPKALDLFKREFDFFDKVTSISGVLFPLPKDERRAGIRSELEKIEMEGED 1740
             VRQ IIDGF+P ALD+F REFDFFDKVTSISGVLFPLPK+ERRAGIR ELEKIEM+G+D
Sbjct: 1681 QVRQHIIDGFSPNALDMFTREFDFFDKVTSISGVLFPLPKEERRAGIRRELEKIEMQGDD 1740

Query: 1741 LYLPTATNKLVCGIQVDSGIPLQSAAKVPIMVTFNVVDRDGDPNNIKPQACIFKVGDDCR 1800
            LYLPTA NKLV GI+VDSGIPLQSAAKVPIM+TFNV+DRDGD +++KPQACIFKVGDDCR
Sbjct: 1741 LYLPTAPNKLVRGIRVDSGIPLQSAAKVPIMITFNVIDRDGDHSDVKPQACIFKVGDDCR 1800

Query: 1801 QDVLALQVISLLRDIFQAVGLNLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGL 1860
            QDVLALQVISLLRDIFQA GLNLYLFPYGVLPTG  RGIIEVVPNTRSRSQMGETTDGGL
Sbjct: 1801 QDVLALQVISLLRDIFQAAGLNLYLFPYGVLPTGAERGIIEVVPNTRSRSQMGETTDGGL 1860

Query: 1861 YEIFQQDYGPVGSPNFEAARENFIVSSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDF 1920
            YEIFQQDYGPVGS  FE ARENF++SSAGYAVASLLLQPKDRHNGNLLFD+VGRLVHIDF
Sbjct: 1861 YEIFQQDYGPVGSTTFETARENFLISSAGYAVASLLLQPKDRHNGNLLFDDVGRLVHIDF 1920

Query: 1921 GFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSETWNLFVRLCVKGYLAARRHMHG 1980
            GFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKS+TW+ FV LCVKGYLAARR M G
Sbjct: 1921 GFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSKTWHQFVSLCVKGYLAARRQMDG 1980

Query: 1981 IINTVLLMLDSGLPCFSRGDPIGNLRKRFHPEMNDREAANFMIRVCTDAYNKWTTAGYDL 2035
            II+TV +ML+SGLPCFSRGDPIGNLRKRFHPEM++REAA+FMI VCTDAYNKWTTAGYDL
Sbjct: 1981 IISTVQMMLESGLPCFSRGDPIGNLRKRFHPEMSEREAAHFMIHVCTDAYNKWTTAGYDL 2028

BLAST of Chy1G008030 vs. ExPASy Swiss-Prot
Match: Q9C680 (Phosphatidylinositol 4-kinase alpha 2 OS=Arabidopsis thaliana OX=3702 GN=PI4KA2 PE=3 SV=1)

HSP 1 Score: 696.8 bits (1797), Expect = 7.2e-199
Identity = 345/420 (82.14%), Postives = 373/420 (88.81%), Query Frame = 0

Query: 1615 LVEGYLLRAAKRSDIFAHILIWHLQGETSLPDSGKDVNSGKNGSFLALLPVVRQRIIDGF 1674
            +VEGYLLRAA+RSDIFAH LIWHLQGE S+ +  KD    KN SF  +L  VRQ I+DGF
Sbjct: 115  MVEGYLLRAAQRSDIFAHTLIWHLQGE-SVEEIVKDGAFDKNASFQEILSDVRQHIVDGF 174

Query: 1675 TPKALDLFKREFDFFDKVTSISGVLFPLPKDERRAGIRSELEKIEMEGEDLYLPTATNKL 1734
            TPKAL+ F REFDFF+KVTSISG L PLPK+ER AGIR ELEKI+M+GEDLYLPTA NKL
Sbjct: 175  TPKALNFFSREFDFFEKVTSISGALLPLPKEERVAGIRRELEKIKMQGEDLYLPTAPNKL 234

Query: 1735 VCGIQVDSGIPLQSAAKVPIMVTFNVVDRDGDPNNIKPQACIFKVGDDCRQDVLALQVIS 1794
            V  IQVDSGIPLQSAAKVPIM+TFNVVD DGD N++KPQACIFKVGDDCRQDVLALQVIS
Sbjct: 235  VRVIQVDSGIPLQSAAKVPIMITFNVVDLDGDHNDVKPQACIFKVGDDCRQDVLALQVIS 294

Query: 1795 LLRDIFQAVGLNLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGP 1854
            LL DIFQAVGLNLYLFPYGVLPT        VVPNTRSRSQMGETTDGGLYEIFQQ+YG 
Sbjct: 295  LLGDIFQAVGLNLYLFPYGVLPT--------VVPNTRSRSQMGETTDGGLYEIFQQNYGL 354

Query: 1855 VGSPNFEAARENFIVSSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFILETSPGG 1914
            VGS  FE AR NF++SSAGYAVASLLLQPKDRHNGNLLFD+VGRLVHIDFGFILETSPGG
Sbjct: 355  VGSTTFETARANFLISSAGYAVASLLLQPKDRHNGNLLFDDVGRLVHIDFGFILETSPGG 414

Query: 1915 NMRFESAHFKLSHEMTQLLDPSGVMKSETWNLFVRLCVKGYLAARRHMHGIINTVLLMLD 1974
            NMRFE+AHFKLSHEMTQLLDPSGVMKS+TW+ FV LCVKGYLAARR+M  II+TV +ML+
Sbjct: 415  NMRFENAHFKLSHEMTQLLDPSGVMKSKTWHQFVSLCVKGYLAARRYMDEIISTVQMMLE 474

Query: 1975 SGLPCFSRGDPIGNLRKRFHPEMNDREAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK 2034
            SGLPCFSRGDPIGNLRKRFHPEM++REAA FMI VCTDAYNKWTTAGYDLIQYLQQG+EK
Sbjct: 475  SGLPCFSRGDPIGNLRKRFHPEMSEREAALFMINVCTDAYNKWTTAGYDLIQYLQQGVEK 525

HSP 2 Score: 112.5 bits (280), Expect = 5.8e-23
Identity = 55/109 (50.46%), Postives = 74/109 (67.89%), Query Frame = 0

Query: 1   MDALIELCDLIAQNPALFSEKLSWICSRCPPSEAILAGSPAISRSQLNAVLAVARLLSKC 60
           M+ L ELCD+I QNP  F +KL WIC +CP S+ + A SP  S+S LNA+LAV R+LSK 
Sbjct: 1   MERLNELCDIIEQNPKQFLDKLPWICQQCPQSKLLRAESPRFSQSHLNAILAVTRILSKI 60

Query: 61  PDSVGLRPKSVVLEFLRSIPLSFSLSFWPQSYGNDAIASFFNEFLNYTS 110
            D+     K VVL+FL+++P SF  SFWP S   ++I++F+  FL Y S
Sbjct: 61  VDTTDENAKFVVLDFLQTVPKSFHRSFWPYSLSLESISAFYCSFLGYVS 109

BLAST of Chy1G008030 vs. ExPASy Swiss-Prot
Match: O02811 (Phosphatidylinositol 4-kinase alpha OS=Bos taurus OX=9913 GN=PI4KA PE=2 SV=2)

HSP 1 Score: 596.3 bits (1536), Expect = 1.3e-168
Identity = 471/1694 (27.80%), Postives = 756/1694 (44.63%), Query Frame = 0

Query: 456  NQGAMFESV----LTTCCEIIESCWTKDRAPVDTFIMGLATSIRDRNDSEEQDDKEKQGV 515
            +Q +M+E +    +   C  +++  T D   V+ F+     S+ +R    ++ DK+   +
Sbjct: 557  SQPSMYEQLRDIAIDNVCRCLKAGLTVDPVIVEAFL----ASLSNRLYISQESDKDAHLI 616

Query: 516  PMQLNVIRLLAKMTVAVNKSEIVDMILPLFIESLEEGDASTPGLLRLQLLDAVSRMATLG 575
            P   + IR L  + VA+  +     ++   ++ L++     P  L + ++D +  +   G
Sbjct: 617  PD--HTIRALGHIAVALRDT---PKVMEPILQILQQKFCQPPSPLDVLIIDQLGCLVITG 676

Query: 576  FEKSYRETIVLMTRSYLSKLSSIGSSESRTVAPEATTERVEILPAGFLHIASGLKSAKLR 635
             +  Y+E   L  +  + K SS+  S ++             +     +IA+ ++   L 
Sbjct: 677  NQYIYQEVWNLFQQISV-KASSVVYSATKDYKDHGYRHCSLAVINALANIAANIQDEHLV 736

Query: 636  LEYRHRLLSLCSDVGLAAESKSGR-----------SGANFLGPLLPAVAEICSDFDPTMD 695
             E    LL L   +GL  +  S R           S A  LG L+P +A +     P  +
Sbjct: 737  DELLMNLLELFVQLGLEGKRASERASEKGPALKASSSAGNLGVLIPVIAVLTRRLPPIKE 796

Query: 696  IEPSLLKLFRNLWFYIALFGLAPPIQNSHLQTKSVSTMLNSVGSTAIALQAVSGPYLWNT 755
             +P L KLFR+ W Y  L G                              AV G  LW  
Sbjct: 797  PKPRLQKLFRDFWLYSVLMGF-----------------------------AVEGSGLWPE 856

Query: 756  QWSSAVQLIARGTPPLVVSSVKWLEDELELNALHNPGSRRGSGNEKAALAQRAALSSALG 815
            +W   V  IA  +P L   S + L   L+ N+           N+    A+ + L S + 
Sbjct: 857  EWYEGVCEIATKSPLLTFPSKEPLRSVLQYNS--------AMKNDTVTPAELSELRSTIV 916

Query: 816  GRVDVA-AMSTISG----VKATYLLAVSFLEIIRFSSNGGILNGGSNVNASRSAFCCVFE 875
              +D A  +S + G      +TYLL+V  LE +R      +L   S+  A      C FE
Sbjct: 917  NLLDPAPEVSALIGKLDFAMSTYLLSVYRLEYMR------VLR--SSDPARFQVMFCYFE 976

Query: 876  YLKTPNLLPAVSQCLTAIVHRAFETAVLWLEDRISDTGNEAEVRDSTLFAHTCYLIKSMS 935
                      + QC+ A+  + F+  +  + ++     NE E     L  H  +L+ S +
Sbjct: 977  DKAIQKDKSGMMQCVIAVADKVFDAFLNMMAEKAKTKENEEE-----LERHAQFLLVSFN 1036

Query: 936  QRDEHVRDIAVNLLTQLRDKFPQVMWNSSCLDSLL-----------FSMHNDAPSTVVTD 995
               + +R +A   L+ L DKFP ++W+ + L ++L             +H D P   + D
Sbjct: 1037 HVHKRIRRVADKYLSGLVDKFPHLLWSGTVLKTMLDILQTLSLSLSADIHKDQPYYDIPD 1096

Query: 996  PAWVVTVRSLYQRVVREWIVK------------SLSYAPCTCQGLLQEKLCKANTWQR-- 1055
              + +TV   Y+   RE IVK            ++ +AP   +  LQE L K   W    
Sbjct: 1097 VPYRITVPDTYE--ARESIVKDFAARCGMILQEAMKWAPTVTKSHLQEYLSKHQNWVSGL 1156

Query: 1056 AQHTPDVICLLSEIRIGTSKNEHWTGIQTANIPAVIAAAAAASGADLKLTEAFNLEVLST 1115
            +QHT         + + T    H+ G            + +     L    A   +  S 
Sbjct: 1157 SQHT--------GLAMATESVLHYAGYN--------KQSTSLGATQLTERPACVKKDYSN 1216

Query: 1116 GMVSATVKCNHAGEIAGMRRLYNSIGGFQTGVAGLGFGQGLQRLITGALPQQPQNEDDSF 1175
             M S  ++  +AGE+ GM R  ++ G                                  
Sbjct: 1217 FMASLNLRNRYAGEVYGMIRFSDATG-----------------------------HTSDL 1276

Query: 1176 NGILIMKFVQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLASESKTNIEGFAQLI 1235
            N +++ +   +L         G G D  ++ +   + TA+L+S+   +         QL+
Sbjct: 1277 NKMMVQELKAAL---------GAG-DAQQYTQAMFKLTAMLISSRDCD--------PQLL 1336

Query: 1236 RLLCWCPAYISTPDAIETGVFVWTWLVSAAPELGSFVLAELVDAWLWTVDTKRGLFASDV 1295
              LCW P  +     +ET +  W WL++    +    + E+  AW  TV+ K GLF++++
Sbjct: 1337 HHLCWGPLQMFNEHGMETALACWEWLLAGKNGVEVPFMREMAGAWQMTVEQKFGLFSAEM 1396

Query: 1296 KYSGP-AAMLRPHLSPGEPEMQPEIDPVEQIIAHKIWLGFFIDRFEVVRHNSVEQLLLFG 1355
            K + P AA       P  PE+ P          H IW+ F + RFE+ ++ S +Q+ +F 
Sbjct: 1397 KEADPLAASEASQPKPCAPEVTP----------HYIWIDFLVQRFEIAKYCSSDQVEIFC 1456

Query: 1356 RLLQGSTK-----PPWNFSRHPAATGSFFTLMLLGLKFCSCQAQGNLQNFKTGLELLEDR 1415
             LLQ S          + +RH AA G  F L+ LGL         N     T   +L ++
Sbjct: 1457 SLLQRSLSLSIGGTAGSMNRHVAAIGPRFKLLTLGLSLLHADVLPN----ATIRNVLREK 1516

Query: 1416 IYRASLGWFAHEPE-----------------------WYDVKHVNFAQ---SEAQSVSIF 1475
            IY  +  +F+  P                        + D K++  +Q    + Q     
Sbjct: 1517 IYSTAFDYFSCPPRFPTQGEKRLREDISVMIKFWTAMFSDKKYLTASQLVPPDNQDTRSN 1576

Query: 1476 LHYLSSERGNSLHSDAKMRGRENGISLIDLNDHYHPVWGHLENYAVGREKRRQLLLMLCQ 1535
            L      R  +           +G+S I                     KRR LLL L  
Sbjct: 1577 LDIAVGSRQQATQGWINTYPLSSGMSTISKKSGMSKKTNRGSQLHKYYMKRRTLLLSLLA 1636

Query: 1536 HEADRLEVWAQP--------------NIKESTPSRPKLTAEKWIEHARTAFSVDPRIAFS 1595
             E +RL  W  P              ++         L+ ++W ++   A+S+ P +A  
Sbjct: 1637 TEIERLITWYNPLSAPELELDQAGESSVANWRSKYISLSEKQWKDNVNLAWSISPHLAVQ 1696

Query: 1596 MVSRFPTNAFLRVEMNQLVQLHILDIRSIPEALPYFVTPKAVDENSELLRQLPHWAACSI 1655
            + +RF     +  E+ +LV+L    +  +PEA+ + VT   +D ++  L  +  WA    
Sbjct: 1697 LPARFKNTEAIGNEVTRLVRLDPGAVSDVPEAIKFLVTWHTIDADAPELSHVLCWAPADP 1756

Query: 1656 TQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQALRYDEGRLVEGYLLRAA 1715
               L + +  Y  HP    Y ++VL S+PP+ + F++PQ+VQALRYD+   V  Y+L AA
Sbjct: 1757 PTGLSYFSSMYPPHPLTAQYGVKVLRSFPPDAILFYIPQIVQALRYDKMGYVREYILWAA 1816

Query: 1716 KRSDIFAHILIWHLQGETSLPDSG--KDVNSGKNGSFLALLPVVRQRIIDGFTPKALDLF 1775
             +S + AH  IW+++    + + G  KD + G       LL  + + I    +  A D +
Sbjct: 1817 SQSQLLAHQFIWNMKTNIYVDEEGHQKDPDIGD------LLEQLVEEITGSLSGPAKDFY 1876

Query: 1776 KREFDFFDKVTSISGVLFPLPK-DERRAGIRSELEKIEMEGEDLYLPTATNKLVCGIQVD 1835
            +REFDFF+K+T++S ++ P PK DER+    S L +++++    YLP+    +V  I   
Sbjct: 1877 QREFDFFNKITNVSAIIKPYPKGDERKKACLSALSEVKVQ-PGCYLPSNPEAIVLDIDYK 1936

Query: 1836 SGIPLQSAAKVPIMVTFNV-------VDRDG----------------DPNNIKPQACIFK 1895
            SG P+QSAAK P +  F V       ++++G                D   I  QA IFK
Sbjct: 1937 SGTPMQSAAKAPYLAKFKVKRCGVSELEKEGLRCRSDPEEEGSMQEADGQKISWQAAIFK 1996

Query: 1896 VGDDCRQDVLALQVISLLRDIFQAVGLNLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGE 1955
            VGDDCRQD+LALQ+I L ++IFQ VGL+L++FPY V+ T PG G+IE +P+  SR Q+G 
Sbjct: 1997 VGDDCRQDMLALQIIDLFKNIFQLVGLDLFVFPYRVVATAPGCGVIECIPDCTSRDQLGR 2056

Query: 1956 TTDGGLYEIFQQDYGPVGSPNFEAARENFIVSSAGYAVASLLLQPKDRHNGNLLFDNVGR 2015
             TD G+Y+ F + YG   +  F+ AR NFI S A Y++   LLQ KDRHNGN++ D  G 
Sbjct: 2057 QTDFGMYDYFTRQYGDESTLAFQQARYNFIRSMAAYSLLLFLLQIKDRHNGNIMLDKKGH 2100

Query: 2016 LVHIDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSETWNLFVRLCVKGYLAA 2033
            L+HIDFGF+ E+SPGGN+ +E    KL+ EM  ++   G M++  +  F+ +CV+GYLA 
Sbjct: 2117 LIHIDFGFMFESSPGGNLGWE-PDIKLTDEMVMIM--GGKMEATPFKWFMEMCVRGYLAV 2100

BLAST of Chy1G008030 vs. ExPASy Swiss-Prot
Match: P42356 (Phosphatidylinositol 4-kinase alpha OS=Homo sapiens OX=9606 GN=PI4KA PE=1 SV=4)

HSP 1 Score: 591.7 bits (1524), Expect = 3.3e-167
Identity = 468/1694 (27.63%), Postives = 750/1694 (44.27%), Query Frame = 0

Query: 456  NQGAMFESV----LTTCCEIIESCWTKDRAPVDTFIMGLATSIRDRNDSEEQDDKEKQGV 515
            +Q +M+E +    +   C  +++  T D   V+ F+     S+ +R    ++ DK+   +
Sbjct: 557  SQPSMYEQLRDIAIDNICRCLKAGLTVDPVIVEAFL----ASLSNRLYISQESDKDAHLI 616

Query: 516  PMQLNVIRLLAKMTVAVNKSEIVDMILPLFIESLEEGDASTPGLLRLQLLDAVSRMATLG 575
            P   + IR L  + VA+  +     ++   ++ L++     P  L + ++D +  +   G
Sbjct: 617  PD--HTIRALGHIAVALRDT---PKVMEPILQILQQKFCQPPSPLDVLIIDQLGCLVITG 676

Query: 576  FEKSYRETIVLMTRSYLSKLSSIGSSESRTVAPEATTERVEILPAGFLHIASGLKSAKLR 635
             +  Y+E   L  +  + K SS+  S ++             +     +IA+ ++   L 
Sbjct: 677  NQYIYQEVWNLFQQISV-KASSVVYSATKDYKDHGYRHCSLAVINALANIAANIQDEHLV 736

Query: 636  LEYRHRLLSLCSDVGLAAESKSGR-----------SGANFLGPLLPAVAEICSDFDPTMD 695
             E    LL L   +GL  +  S R           S A  LG L+P +A +     P  +
Sbjct: 737  DELLMNLLELFVQLGLEGKRASERASEKGPALKASSSAGNLGVLIPVIAVLTRRLPPIKE 796

Query: 696  IEPSLLKLFRNLWFYIALFGLAPPIQNSHLQTKSVSTMLNSVGSTAIALQAVSGPYLWNT 755
             +P L KLFR+ W Y  L G                              AV G  LW  
Sbjct: 797  AKPRLQKLFRDFWLYSVLMGF-----------------------------AVEGSGLWPE 856

Query: 756  QWSSAVQLIARGTPPLVVSSVKWLEDELELNALHNPGSRRGSGNEKAALAQRAALSSALG 815
            +W   V  IA  +P L   S + L   L+ N+           N+    A+ + L S + 
Sbjct: 857  EWYEGVCEIATKSPLLTFPSKEPLRSVLQYNS--------AMKNDTVTPAELSELRSTII 916

Query: 816  GRVD-----VAAMSTISGVKATYLLAVSFLEIIRFSSNGGILNGGSNVNASRSAFCCVFE 875
              +D      A ++ +    +TYLL+V  LE +R   +       ++ +  +  F C FE
Sbjct: 917  NLLDPPPEVSALINKLDFAMSTYLLSVYRLEYMRVLRS-------TDPDRFQVMF-CYFE 976

Query: 876  YLKTPNLLPAVSQCLTAIVHRAFETAVLWLEDRISDTGNEAEVRDSTLFAHTCYLIKSMS 935
                      + QC+ A+  + F+  +  + D+     NE E     L  H  +L+ + +
Sbjct: 977  DKAIQKDKSGMMQCVIAVADKVFDAFLNMMADKAKTKENEEE-----LERHAQFLLVNFN 1036

Query: 936  QRDEHVRDIAVNLLTQLRDKFPQVMWNSSCLDSLL-----------FSMHNDAPSTVVTD 995
               + +R +A   L+ L DKFP ++W+ + L ++L             +H D P   + D
Sbjct: 1037 HIHKRIRRVADKYLSGLVDKFPHLLWSGTVLKTMLDILQTLSLSLSADIHKDQPYYDIPD 1096

Query: 996  PAWVVTVRSLYQRVVREWIVK------------SLSYAPCTCQGLLQEKLCKANTWQR-- 1055
              + +TV   Y+   RE IVK            ++ +AP   +  LQE L K   W    
Sbjct: 1097 APYRITVPDTYE--ARESIVKDFAARCGMILQEAMKWAPTVTKSHLQEYLNKHQNWVSGL 1156

Query: 1056 AQHTPDVICLLSEIRIGTSKNEHWTGIQTANIPAVIAAAAAASGADLKLTEAFNLEVLST 1115
            +QHT         + + T    H+ G    N               L    A   +  S 
Sbjct: 1157 SQHT--------GLAMATESILHFAGYNKQN--------TTLGATQLSERPACVKKDYSN 1216

Query: 1116 GMVSATVKCNHAGEIAGMRRLYNSIGGFQTGVAGLGFGQGLQRLITGALPQQPQNEDDSF 1175
             M S  ++  +AGE+ GM R   + G  Q         Q L   +  + PQ         
Sbjct: 1217 FMASLNLRNRYAGEVYGMIRFSGTTG--QMSDLNKMMVQDLHSALDRSHPQH-------- 1276

Query: 1176 NGILIMKFVQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLASESKTNIEGFAQLI 1235
                                         + +   + TA+L+S+   +         QL+
Sbjct: 1277 -----------------------------YTQAMFKLTAMLISSKDCD--------PQLL 1336

Query: 1236 RLLCWCPAYISTPDAIETGVFVWTWLVSAAPELGSFVLAELVDAWLWTVDTKRGLFASDV 1295
              LCW P  +     +ET +  W WL++    +    + E+  AW  TV+ K GLF++++
Sbjct: 1337 HHLCWGPLRMFNEHGMETALACWEWLLAGKDGVEVPFMREMAGAWHMTVEQKFGLFSAEI 1396

Query: 1296 KYSGP-AAMLRPHLSPGEPEMQPEIDPVEQIIAHKIWLGFFIDRFEVVRHNSVEQLLLFG 1355
            K + P AA       P  PE+ P          H IW+ F + RFE+ ++ S +Q+ +F 
Sbjct: 1397 KEADPLAASEASQPKPCPPEVTP----------HYIWIDFLVQRFEIAKYCSSDQVEIFS 1456

Query: 1356 RLLQGSTK-----PPWNFSRHPAATGSFFTLMLLGLKFCSCQAQGNLQNFKTGLELLEDR 1415
             LLQ S          + +RH AA G  F L+ LGL         N     T   +L ++
Sbjct: 1457 SLLQRSMSLNIGGAKGSMNRHVAAIGPRFKLLTLGLSLLHADVVPN----ATIRNVLREK 1516

Query: 1416 IYRASLGWFAHEPE-----------------------WYDVKHVNFAQ---SEAQSVSIF 1475
            IY  +  +F+  P+                       + D K++  +Q    + Q     
Sbjct: 1517 IYSTAFDYFSCPPKFPTQGEKRLREDISIMIKFWTAMFSDKKYLTASQLVPPDNQDTRSN 1576

Query: 1476 LHYLSSERGNSLHSDAKMRGRENGISLIDLNDHYHPVWGHLENYAVGREKRRQLLLMLCQ 1535
            L      R  +           +G+S I                     KRR LLL L  
Sbjct: 1577 LDITVGSRQQATQGWINTYPLSSGMSTISKKSGMSKKTNRGSQLHKYYMKRRTLLLSLLA 1636

Query: 1536 HEADRLEVWAQP--------------NIKESTPSRPKLTAEKWIEHARTAFSVDPRIAFS 1595
             E +RL  W  P              ++         L+ ++W ++   A+S+ P +A  
Sbjct: 1637 TEIERLITWYNPLSAPELELDQAGENSVANWRSKYISLSEKQWKDNVNLAWSISPYLAVQ 1696

Query: 1596 MVSRFPTNAFLRVEMNQLVQLHILDIRSIPEALPYFVTPKAVDENSELLRQLPHWAACSI 1655
            + +RF     +  E+ +LV+L    +  +PEA+ + VT   +D ++  L  +  WA    
Sbjct: 1697 LPARFKNTEAIGNEVTRLVRLDPGAVSDVPEAIKFLVTWHTIDADAPELSHVLCWAPTDP 1756

Query: 1656 TQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQALRYDEGRLVEGYLLRAA 1715
               L + +  Y  HP    Y ++VL S+PP+ + F++PQ+VQALRYD+   V  Y+L AA
Sbjct: 1757 PTGLSYFSSMYPPHPLTAQYGVKVLRSFPPDAILFYIPQIVQALRYDKMGYVREYILWAA 1816

Query: 1716 KRSDIFAHILIWHLQGETSLPDSG--KDVNSGKNGSFLALLPVVRQRIIDGFTPKALDLF 1775
             +S + AH  IW+++    L + G  KD + G       LL  + + I    +  A D +
Sbjct: 1817 SKSQLLAHQFIWNMKTNIYLDEEGHQKDPDIGD------LLDQLVEEITGSLSGPAKDFY 1876

Query: 1776 KREFDFFDKVTSISGVLFPLPK-DERRAGIRSELEKIEMEGEDLYLPTATNKLVCGIQVD 1835
            +REFDFF+K+T++S ++ P PK DER+    S L +++++    YLP+    +V  I   
Sbjct: 1877 QREFDFFNKITNVSAIIKPYPKGDERKKACLSALSEVKVQ-PGCYLPSNPEAIVLDIDYK 1936

Query: 1836 SGIPLQSAAKVPIMVTFNV-------VDRDG----------------DPNNIKPQACIFK 1895
            SG P+QSAAK P +  F V       ++++G                D   I  QA IFK
Sbjct: 1937 SGTPMQSAAKAPYLAKFKVKRCGVSELEKEGLRCRSDSEDECSTQEADGQKISWQAAIFK 1996

Query: 1896 VGDDCRQDVLALQVISLLRDIFQAVGLNLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGE 1955
            VGDDCRQD+LALQ+I L ++IFQ VGL+L++FPY V+ T PG G+IE +P+  SR Q+G 
Sbjct: 1997 VGDDCRQDMLALQIIDLFKNIFQLVGLDLFVFPYRVVATAPGCGVIECIPDCTSRDQLGR 2056

Query: 1956 TTDGGLYEIFQQDYGPVGSPNFEAARENFIVSSAGYAVASLLLQPKDRHNGNLLFDNVGR 2015
             TD G+Y+ F + YG   +  F+ AR NFI S A Y++   LLQ KDRHNGN++ D  G 
Sbjct: 2057 QTDFGMYDYFTRQYGDESTLAFQQARYNFIRSMAAYSLLLFLLQIKDRHNGNIMLDKKGH 2100

Query: 2016 LVHIDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSETWNLFVRLCVKGYLAA 2033
            ++HIDFGF+ E+SPGGN+ +E    KL+ EM  ++   G M++  +  F+ +CV+GYLA 
Sbjct: 2117 IIHIDFGFMFESSPGGNLGWE-PDIKLTDEMVMIM--GGKMEATPFKWFMEMCVRGYLAV 2100

BLAST of Chy1G008030 vs. ExPASy Swiss-Prot
Match: O08662 (Phosphatidylinositol 4-kinase alpha OS=Rattus norvegicus OX=10116 GN=Pi4ka PE=1 SV=2)

HSP 1 Score: 590.5 bits (1521), Expect = 7.3e-167
Identity = 471/1693 (27.82%), Postives = 758/1693 (44.77%), Query Frame = 0

Query: 456  NQGAMFESV----LTTCCEIIESCWTKDRAPVDTFIMGLATSIRDRNDSEEQDDKEKQGV 515
            NQ +M+E +    +   C  +++  T D   V+ F+     S+ +R    ++ DK+   +
Sbjct: 551  NQPSMYEQLRDIAIDNICRCLKAGLTVDPVIVEAFL----ASLSNRLYISQESDKDAHLI 610

Query: 516  PMQLNVIRLLAKMTVAVNKSEIVDMILPLFIESLEEGDASTPGLLRLQLLDAVSRMATLG 575
            P   + IR L  + VA+  +     ++   ++ L++     P  L + ++D +  +   G
Sbjct: 611  PD--HTIRALGHIAVALRDT---PKVMEPILQILQQKFCQPPSPLDVLIIDQLGCLVITG 670

Query: 576  FEKSYRETIVLMTRSYLSKLSSIGSSESRTVAPEATTERVEILPAGFLHIASGLKSAKLR 635
             +  Y+E   L  +  + K SS+  S ++             +     +IA+ ++   L 
Sbjct: 671  NQYIYQEVWNLFQQISV-KASSVVYSATKDYKDHGYRHCSLAVINALANIAANIQEEHLV 730

Query: 636  LEYRHRLLSLCSDVGLAAESKSGR-----------SGANFLGPLLPAVAEICSDFDPTMD 695
             E    LL L   +GL  +  S R           S A  LG L+P +A +     P  +
Sbjct: 731  DELLMNLLELFVQLGLEGKRASERASEKGPALKASSSAGNLGVLIPVIAVLTRRLPPIKE 790

Query: 696  IEPSLLKLFRNLWFYIALFGLAPPIQNSHLQTKSVSTMLNSVGSTAIALQAVSGPYLWNT 755
             +P L KLFR+ W Y  L G                              AV G  LW  
Sbjct: 791  AKPRLQKLFRDFWLYSVLMGF-----------------------------AVEGSGLWPE 850

Query: 756  QWSSAVQLIARGTPPLVVSSVKWLEDELELN-ALHNPGSRRGSGNEKAALAQRAALSSAL 815
            +W   V  IA  +P L   S + L   L+ N A+ N        NE      R+ + + L
Sbjct: 851  EWYEGVCEIATKSPLLTFPSKEPLRSVLQYNSAMKNDTVTPAELNE-----LRSTIINLL 910

Query: 816  GGRVDVAAM-STISGVKATYLLAVSFLEIIRFSSNGGILNGGSNVNASRSAFCCVFEYLK 875
                +V+A+ + +    +TYLL+V  LE +R   +       ++ +  +  F C FE   
Sbjct: 911  DPPPEVSALINKLDFAMSTYLLSVYRLEYMRVLRS-------TDPDRFQVMF-CYFEDKA 970

Query: 876  TPNLLPAVSQCLTAIVHRAFETAVLWLEDRISDTGNEAEVRDSTLFAHTCYLIKSMSQRD 935
                   + QC+ A+  + F+  +  + ++     NE E     L  H  +L+ + +   
Sbjct: 971  IQKDKSGMMQCVIAVADKVFDAFLNMMAEKAKTKENEEE-----LERHAQFLLVNFNHIH 1030

Query: 936  EHVRDIAVNLLTQLRDKFPQVMWNSSCLDSLL-----------FSMHNDAPSTVVTDPAW 995
            + +R +A   L+ L DKFP ++W+ + L ++L             +H D P   + D  +
Sbjct: 1031 KRIRRVADKYLSGLVDKFPHLLWSGTVLKTMLDILQTLSLSLSADIHKDQPYYDIPDAPY 1090

Query: 996  VVTVRSLYQRVVREWIVK------------SLSYAPCTCQGLLQEKLCKANTWQR--AQH 1055
             +TV   Y+   RE IVK            ++ +AP   +  LQE L K   W    +QH
Sbjct: 1091 RITVPDTYE--ARESIVKDFAARCGMILQEAMKWAPTVTKSHLQEYLNKHQNWVSGLSQH 1150

Query: 1056 TPDVICLLSEIRIGTSKNEHWTGIQTANIPAVIAAAAAASGADLKLTE--AFNLEVLSTG 1115
            T         + + T    H+ G    N    +           +LTE  A   +  S  
Sbjct: 1151 T--------GLAMATESILHFAGYNKQNTTLGVT----------QLTERPACVKKDYSNF 1210

Query: 1116 MVSATVKCNHAGEIAGMRRLYNSIGGFQTGVAGLGFGQGLQRLITGALPQQPQNEDDSFN 1175
            M S  ++  +AGE+ GM R   + G        +     +Q LIT      PQ+      
Sbjct: 1211 MASLNLRNRYAGEVHGMIRFSGATGQMSDLNKMM-----VQDLITALDHSHPQH------ 1270

Query: 1176 GILIMKFVQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLASESKTNIEGFAQLIR 1235
                                        + +   + TA+L+S+   +         QL+ 
Sbjct: 1271 ----------------------------YTQAMFKLTAMLISSKDCD--------PQLLH 1330

Query: 1236 LLCWCPAYISTPDAIETGVFVWTWLVSAAPELGSFVLAELVDAWLWTVDTKRGLFASDVK 1295
             LCW P  +     +ET +  W WL++    +    + E+  AW  TV+ K GLF+++ K
Sbjct: 1331 HLCWGPLRMFNEHGMETALACWEWLLAGKNGVEVPFMREMAGAWHMTVEQKFGLFSAETK 1390

Query: 1296 YSGP-AAMLRPHLSPGEPEMQPEIDPVEQIIAHKIWLGFFIDRFEVVRHNSVEQLLLFGR 1355
             + P AA       P  PE+ P          H IW+ F + RFE+ ++ S +Q+ +F  
Sbjct: 1391 EADPLAASEASQPRPCPPEVTP----------HYIWIDFLVQRFEIAKYCSSDQVEIFSS 1450

Query: 1356 LLQGSTK-----PPWNFSRHPAATGSFFTLMLLGLKFCSCQAQGNLQNFKTGLELLEDRI 1415
            LLQ S          + +RH AA G  F L+ LGL         N     T   +L ++I
Sbjct: 1451 LLQRSMSLHIGGARGSMNRHVAAIGPRFKLLTLGLSLLHADVVPN----ATIRNVLREKI 1510

Query: 1416 YRASLGWFAHEPE-----------------------WYDVKHVNFAQ---SEAQSVSIFL 1475
            Y  +  +F+  P+                       + D K++  +Q    + Q     L
Sbjct: 1511 YSTAFDYFSCPPKFPTQGEKRLREDISIMIKFWTAMFSDKKYLTASQLVPPDNQDTRSNL 1570

Query: 1476 HYLSSERGNSLHSDAKMRGRENGISLIDLNDHYHPVWGHLENYAVGREKRRQLLLMLCQH 1535
                  R  +           +G+S I                     KRR LLL L   
Sbjct: 1571 DITVGSRQQATQGWINTYPLSSGMSTISKKSGMSKKTNRGSQLHKYYMKRRTLLLSLLAT 1630

Query: 1536 EADRLEVWAQP--------------NIKESTPSRPKLTAEKWIEHARTAFSVDPRIAFSM 1595
            E +RL  W  P              ++         L+ ++W ++   A+++ P +A  +
Sbjct: 1631 EIERLITWYNPLSAPELELDQAGENSVANWRSKYISLSEKQWKDNVNLAWTISPYLAVQL 1690

Query: 1596 VSRFPTNAFLRVEMNQLVQLHILDIRSIPEALPYFVTPKAVDENSELLRQLPHWAACSIT 1655
             +RF     +  E+ +LV+L    +  +PEA+ + VT   +D ++  L  +  WA     
Sbjct: 1691 PARFKNTEAIGNEVTRLVRLDPGAVSDVPEAIKFLVTWHTIDADAPELSHVLCWAPTDPP 1750

Query: 1656 QALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQALRYDEGRLVEGYLLRAAK 1715
              L + +  Y  HP    Y ++VL S+PP+ + F++PQ+VQALRYD+   V  Y+L AA 
Sbjct: 1751 TGLSYFSSMYPPHPLTAQYGVKVLRSFPPDAILFYIPQIVQALRYDKMGYVREYILWAAA 1810

Query: 1716 RSDIFAHILIWHLQGETSLPDSG--KDVNSGKNGSFLALLPVVRQRIIDGFTPKALDLFK 1775
            +S + AH  IW+++    L + G  KD + G       LL  + + I    +  A D ++
Sbjct: 1811 KSQLLAHQFIWNMKTNIYLDEEGHQKDPDIGD------LLEQLVEEITGSLSGPAKDFYQ 1870

Query: 1776 REFDFFDKVTSISGVLFPLPK-DERRAGIRSELEKIEMEGEDLYLPTATNKLVCGIQVDS 1835
            REFDFF+K+T++S ++ P PK DER+    S L +++++    YLP+    +V  I   S
Sbjct: 1871 REFDFFNKITNVSAIIKPYPKGDERKKACLSALSEVKVQ-PGCYLPSNPEAIVLDIDYKS 1930

Query: 1836 GIPLQSAAKVPIMVTFNV-------VDRDG----------------DPNNIKPQACIFKV 1895
            G P+QSAAK P +  F V       ++++G                D   I  QA IFKV
Sbjct: 1931 GTPMQSAAKAPYLAKFKVKRCGVSELEKEGLQCRSDTEDECRRQEADGKKICWQAAIFKV 1990

Query: 1896 GDDCRQDVLALQVISLLRDIFQAVGLNLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGET 1955
            GDDCRQD+LALQ+I L ++IFQ VGL+L++FPY V+ T PG G+IE +P+  SR Q+G  
Sbjct: 1991 GDDCRQDMLALQIIDLFKNIFQLVGLDLFVFPYRVVATAPGCGVIECIPDCTSRDQLGRQ 2050

Query: 1956 TDGGLYEIFQQDYGPVGSPNFEAARENFIVSSAGYAVASLLLQPKDRHNGNLLFDNVGRL 2015
            TD G+Y+ F + YG   +  F+ AR NFI S A Y++   LLQ KDRHNGN++ D  G +
Sbjct: 2051 TDFGMYDYFTRQYGDESTLAFQQARYNFIRSMAAYSLLLFLLQIKDRHNGNIMLDKKGHI 2094

Query: 2016 VHIDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSETWNLFVRLCVKGYLAAR 2033
            +HIDFGF+ E+SPGGN+ +E    KL+ EM  ++   G M++  +  F+ +CV+GYLA R
Sbjct: 2111 IHIDFGFMFESSPGGNLGWE-PDIKLTDEMVMIM--GGKMEATPFKWFMEMCVRGYLAVR 2094

BLAST of Chy1G008030 vs. ExPASy TrEMBL
Match: A0A1S3BGG8 (phosphatidylinositol 4-kinase alpha 1-like OS=Cucumis melo OX=3656 GN=LOC103489597 PE=4 SV=1)

HSP 1 Score: 3942.9 bits (10224), Expect = 0.0e+00
Identity = 1993/2037 (97.84%), Postives = 2009/2037 (98.63%), Query Frame = 0

Query: 1    MDALIELCDLIAQNPALFSEKLSWICSRCPPSEAILAGSPAISRSQLNAVLAVARLLSKC 60
            MDALIELCDLIAQNPALFSEKLSWICSRCPPSEAILAGSP ISRSQLNAVLAVARLLSKC
Sbjct: 1    MDALIELCDLIAQNPALFSEKLSWICSRCPPSEAILAGSPTISRSQLNAVLAVARLLSKC 60

Query: 61   PDSVGLRPKSVVLEFLRSIPLSFSLSFWPQSYGNDAIASFFNEFLNYTSKACALSTDFAT 120
            PDSVGLRPKSVVLEFLRSIPLSFSLSFWPQSYG+DAIASFFNEFLNYTSKAC LSTDFAT
Sbjct: 61   PDSVGLRPKSVVLEFLRSIPLSFSLSFWPQSYGHDAIASFFNEFLNYTSKACELSTDFAT 120

Query: 121  EVSGFSSEVVLSAINDCSEGSAISRAFLVALSKSFLPIIPSDADKLVSCILDRFLISEAA 180
            EVSGFSSEVVLSAINDCSEGSAISRAFLVAL+KSFLPIIPSDADKLVSCILDRFLISEA 
Sbjct: 121  EVSGFSSEVVLSAINDCSEGSAISRAFLVALTKSFLPIIPSDADKLVSCILDRFLISEAI 180

Query: 181  PGTPREHNQANSEPSSSQSSPLSVSHQPSNGGLSPGNENGQVSGSLSSGASRITDDATSA 240
            P TPREHNQANSEPSSSQSSPLSVSHQPSNGGLSPGNENGQVSGSLSSGASRITDDATSA
Sbjct: 181  PETPREHNQANSEPSSSQSSPLSVSHQPSNGGLSPGNENGQVSGSLSSGASRITDDATSA 240

Query: 241  SSRGSGMMNGNSILWKSGLEQLGMNLGFSEGGGVAFVRQQVALFVDESIENLEKQEIAFK 300
            SSRGSGMMNGNSILWKSGLEQLGMNLGFSEGGGVAFVRQQVALF DESIENLEKQEIAFK
Sbjct: 241  SSRGSGMMNGNSILWKSGLEQLGMNLGFSEGGGVAFVRQQVALFEDESIENLEKQEIAFK 300

Query: 301  LMTHILDNSSFDARLWEQMRALAKKQLQTLPTFLKIKKRDWSEQGSVLKARINTKLLVYQ 360
            LMTHILDNSSFDARLWEQM+ LAKKQLQTLPTFLKIKKRDWSEQGSVLKARINTKLLVYQ
Sbjct: 301  LMTHILDNSSFDARLWEQMKTLAKKQLQTLPTFLKIKKRDWSEQGSVLKARINTKLLVYQ 360

Query: 361  AAARLKMKTVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFSSLLTGL 420
            AAARLKMKTVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELF SLLTGL
Sbjct: 361  AAARLKMKTVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFGSLLTGL 420

Query: 421  AQIAVARGGQPLRVLLIRLKPLVLTVCVQADTWGTNQGAMFESVLTTCCEIIESCWTKDR 480
            AQIAVARGGQPLRVLLIRLKPLVLTVCVQADTWGTNQGAMFESVLTTCCEIIESCWTKDR
Sbjct: 421  AQIAVARGGQPLRVLLIRLKPLVLTVCVQADTWGTNQGAMFESVLTTCCEIIESCWTKDR 480

Query: 481  APVDTFIMGLATSIRDRNDSEEQDDKEKQGVPMQLNVIRLLAKMTVAVNKSEIVDMILPL 540
            APVDTFIMGLATSIRDRNDSEEQDDKEKQGVPMQLNVIRLLAKMTVAVNKSEIVDMILPL
Sbjct: 481  APVDTFIMGLATSIRDRNDSEEQDDKEKQGVPMQLNVIRLLAKMTVAVNKSEIVDMILPL 540

Query: 541  FIESLEEGDASTPGLLRLQLLDAVSRMATLGFEKSYRETIVLMTRSYLSKLSSIGSSESR 600
            FIESLEEGDASTPGLLRLQLLDAVSRMATLGFEKSYRETIVLMTRSYLSKLSSIGSSESR
Sbjct: 541  FIESLEEGDASTPGLLRLQLLDAVSRMATLGFEKSYRETIVLMTRSYLSKLSSIGSSESR 600

Query: 601  TVAPEATTERVEILPAGFLHIASGLKSAKLRLEYRHRLLSLCSDVGLAAESKSGRSGANF 660
            TVAPEATTERVEILPAGFLHIA+GLKSAKLRLEYRHRLLSLCSDVGLAAESKSGRSGA+F
Sbjct: 601  TVAPEATTERVEILPAGFLHIANGLKSAKLRLEYRHRLLSLCSDVGLAAESKSGRSGADF 660

Query: 661  LGPLLPAVAEICSDFDPTMDIEPSLLKLFRNLWFYIALFGLAPPIQNSHLQTKSVSTMLN 720
            LGPLLPAVAEICSDFDPTM+IEPSLLKLFRNLWFYIALFGLAPPIQ S LQTKSVSTMLN
Sbjct: 661  LGPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQKSQLQTKSVSTMLN 720

Query: 721  SVGSTAIALQAVSGPYLWNTQWSSAVQLIARGTPPLVVSSVKWLEDELELNALHNPGSRR 780
            SVGSTAIALQAVSGPYLWNTQWSSAVQ IARGTPPLVVSSVKWLEDELELNALHNPGSRR
Sbjct: 721  SVGSTAIALQAVSGPYLWNTQWSSAVQFIARGTPPLVVSSVKWLEDELELNALHNPGSRR 780

Query: 781  GSGNEKAALAQRAALSSALGGRVDVAAMSTISGVKATYLLAVSFLEIIRFSSNGGILNGG 840
            GSGNEKAALAQRAALS+ALGGRVDVAAMSTISGVKATYLLAVSFLEIIRFSSNGGILNGG
Sbjct: 781  GSGNEKAALAQRAALSAALGGRVDVAAMSTISGVKATYLLAVSFLEIIRFSSNGGILNGG 840

Query: 841  SNVNASRSAFCCVFEYLKTPNLLPAVSQCLTAIVHRAFETAVLWLEDRISDTGNEAEVRD 900
            SNVNASRSAFCCVFEYLKTPNLLPAVSQCLTAIVHRAFETAV WLEDRISDTGNEAE RD
Sbjct: 841  SNVNASRSAFCCVFEYLKTPNLLPAVSQCLTAIVHRAFETAVSWLEDRISDTGNEAEARD 900

Query: 901  STLFAHTCYLIKSMSQRDEHVRDIAVNLLTQLRDKFPQVMWNSSCLDSLLFSMHNDAPST 960
            STLFAHTCYLIKSMSQRDEHVRDIAVNLLTQLR+KFPQVMWNSSCLDSLLFSMHNDAPST
Sbjct: 901  STLFAHTCYLIKSMSQRDEHVRDIAVNLLTQLREKFPQVMWNSSCLDSLLFSMHNDAPST 960

Query: 961  VVTDPAWVVTVRSLYQRVVREWIVKSLSYAPCTCQGLLQEKLCKANTWQRAQHTPDVICL 1020
            VV+DPAWVVTVRSLYQRVVREWIVKSLSYAPCTCQGLLQEKLCKANTWQRAQHTPDVI L
Sbjct: 961  VVSDPAWVVTVRSLYQRVVREWIVKSLSYAPCTCQGLLQEKLCKANTWQRAQHTPDVISL 1020

Query: 1021 LSEIRIGTSKNEHWTGIQTANIPAVIAAAAAASGADLKLTEAFNLEVLSTGMVSATVKCN 1080
            LSEIRIGTSKNEHWTG QTANIPAVI AAAAASGADLKLTEAFNLEVLSTGMVSATVKCN
Sbjct: 1021 LSEIRIGTSKNEHWTGTQTANIPAVITAAAAASGADLKLTEAFNLEVLSTGMVSATVKCN 1080

Query: 1081 HAGEIAGMRRLYNSIGGFQTGVA---GLGFGQGLQRLITGALPQQPQNEDDSFNGILIMK 1140
            HAGEIAGMRRLYNSIGGFQTGVA   GLGFGQGLQRLITGALPQQPQNEDDSFNGILIMK
Sbjct: 1081 HAGEIAGMRRLYNSIGGFQTGVAGSTGLGFGQGLQRLITGALPQQPQNEDDSFNGILIMK 1140

Query: 1141 FVQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLASESKTNIEGFAQLIRLLCWCP 1200
            FVQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNL SESKTNIEGFAQLIRLLCWCP
Sbjct: 1141 FVQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLDSESKTNIEGFAQLIRLLCWCP 1200

Query: 1201 AYISTPDAIETGVFVWTWLVSAAPELGSFVLAELVDAWLWTVDTKRGLFASDVKYSGPAA 1260
            AYISTPDAIETGVF+WTWLVSAAPELGSFVLAELVDAWLWT+DTKRGLFASD+KYSGPAA
Sbjct: 1201 AYISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRGLFASDIKYSGPAA 1260

Query: 1261 MLRPHLSPGEPEMQPEIDPVEQIIAHKIWLGFFIDRFEVVRHNSVEQLLLFGRLLQGSTK 1320
            MLRPHLSPGEPEMQPEIDPVEQIIAH+IWLGFFIDRFEVVRHNSVEQLLLFGRLLQGSTK
Sbjct: 1261 MLRPHLSPGEPEMQPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQLLLFGRLLQGSTK 1320

Query: 1321 PPWNFSRHPAATGSFFTLMLLGLKFCSCQAQGNLQNFKTGLELLEDRIYRASLGWFAHEP 1380
            PPWNFSRHPAATGSFFTLMLLGLKFCSCQAQGNLQNFKTGLELLEDRIYRASLGWFAHEP
Sbjct: 1321 PPWNFSRHPAATGSFFTLMLLGLKFCSCQAQGNLQNFKTGLELLEDRIYRASLGWFAHEP 1380

Query: 1381 EWYDVKHVNFAQSEAQSVSIFLHYLSSERGNSLHSDAKMRGRENGISLIDLNDHYHPVWG 1440
            EWYDVKHVNFAQSEAQSVSIFLHYLSSERGNSL SDAK+RGRENGISLID NDHYHPVWG
Sbjct: 1381 EWYDVKHVNFAQSEAQSVSIFLHYLSSERGNSLQSDAKIRGRENGISLIDSNDHYHPVWG 1440

Query: 1441 HLENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTPSRPKLTAEKWIEHARTAFSV 1500
            HLENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTP RPKLTAEKWIEHARTAFSV
Sbjct: 1441 HLENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTPYRPKLTAEKWIEHARTAFSV 1500

Query: 1501 DPRIAFSMVSRFPTNAFLRVEMNQLVQLHILDIRSIPEALPYFVTPKAVDENSELLRQLP 1560
            DPRIAFSMVSRFPTNAFLRVEMNQLVQLHILDIRSIPEALPYFVTPKAVDENSELLRQLP
Sbjct: 1501 DPRIAFSMVSRFPTNAFLRVEMNQLVQLHILDIRSIPEALPYFVTPKAVDENSELLRQLP 1560

Query: 1561 HWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQALRYDEGRLVE 1620
            HWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQALRYDEGRLVE
Sbjct: 1561 HWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQALRYDEGRLVE 1620

Query: 1621 GYLLRAAKRSDIFAHILIWHLQGETSLPDSGKDVNSGKNGSFLALLPVVRQRIIDGFTPK 1680
            GYLLRAAKRSDIFAHILIWHLQGETS PDSGKDVNSGKNGSFLALLPVVRQ IIDGFTPK
Sbjct: 1621 GYLLRAAKRSDIFAHILIWHLQGETSSPDSGKDVNSGKNGSFLALLPVVRQHIIDGFTPK 1680

Query: 1681 ALDLFKREFDFFDKVTSISGVLFPLPKDERRAGIRSELEKIEMEGEDLYLPTATNKLVCG 1740
            ALDLF+REFDFFDKVTSISGVLFPLPKDERRAGIR ELEKIEM+GEDLYLPTATNKLV G
Sbjct: 1681 ALDLFRREFDFFDKVTSISGVLFPLPKDERRAGIRRELEKIEMDGEDLYLPTATNKLVRG 1740

Query: 1741 IQVDSGIPLQSAAKVPIMVTFNVVDRDGDPNNIKPQACIFKVGDDCRQDVLALQVISLLR 1800
            IQVDSGIPLQSAAKVPIMVTFNVVDRDGDPNNIKPQACIFKVGDDCRQDVLALQVISLLR
Sbjct: 1741 IQVDSGIPLQSAAKVPIMVTFNVVDRDGDPNNIKPQACIFKVGDDCRQDVLALQVISLLR 1800

Query: 1801 DIFQAVGLNLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGPVGS 1860
            DIFQAVGLNLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGPVGS
Sbjct: 1801 DIFQAVGLNLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGPVGS 1860

Query: 1861 PNFEAARENFIVSSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFILETSPGGNMR 1920
            PNFEAARENFIVSSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFILETSPGGNMR
Sbjct: 1861 PNFEAARENFIVSSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFILETSPGGNMR 1920

Query: 1921 FESAHFKLSHEMTQLLDPSGVMKSETWNLFVRLCVKGYLAARRHMHGIINTVLLMLDSGL 1980
            FESAHFKLSHEMTQLLDPSGVMKSETWNLFV LCVKGYLAARRHM GIINTVLLMLDSGL
Sbjct: 1921 FESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMDGIINTVLLMLDSGL 1980

Query: 1981 PCFSRGDPIGNLRKRFHPEMNDREAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK 2035
            PCFSRGDPIGNLRKRFHPEMNDREAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK
Sbjct: 1981 PCFSRGDPIGNLRKRFHPEMNDREAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK 2037

BLAST of Chy1G008030 vs. ExPASy TrEMBL
Match: A0A6J1I6A0 (phosphatidylinositol 4-kinase alpha 1-like OS=Cucurbita maxima OX=3661 GN=LOC111471091 PE=4 SV=1)

HSP 1 Score: 3830.8 bits (9933), Expect = 0.0e+00
Identity = 1933/2037 (94.89%), Postives = 1980/2037 (97.20%), Query Frame = 0

Query: 1    MDALIELCDLIAQNPALFSEKLSWICSRCPPSEAILAGSPAISRSQLNAVLAVARLLSKC 60
            MDALIELCDLIAQNPALFSEKLSWICSRCPPSEAILAGSPAISRSQLNAVLAVARLLSKC
Sbjct: 1    MDALIELCDLIAQNPALFSEKLSWICSRCPPSEAILAGSPAISRSQLNAVLAVARLLSKC 60

Query: 61   PDSVGLRPKSVVLEFLRSIPLSFSLSFWPQSYGNDAIASFFNEFLNYTSKACALSTDFAT 120
            P+SVGLRPKSVVLEFLRSIPLSFSLSFWPQS+GNDAIASFF EFLNYTSKAC LSTDFA 
Sbjct: 61   PESVGLRPKSVVLEFLRSIPLSFSLSFWPQSFGNDAIASFFTEFLNYTSKACELSTDFAM 120

Query: 121  EVSGFSSEVVLSAINDCSEGSAISRAFLVALSKSFLPIIPSDADKLVSCILDRFLISEAA 180
            EVSGF+SEVVLSAINDCSEGSAISRAFLVALSKSFLPIIPSDADKLVSCILD FLISE  
Sbjct: 121  EVSGFTSEVVLSAINDCSEGSAISRAFLVALSKSFLPIIPSDADKLVSCILDLFLISEVV 180

Query: 181  PGTPREHNQANSEPSSSQSSPLSVSHQPSNGGLSPGNENGQVSGSLSSGASRITDDATSA 240
            PGTPRE  QANSEPSSSQSSPLSVSHQPSNGGLSPGNENGQVSGSLSSGASRITDDATSA
Sbjct: 181  PGTPREQIQANSEPSSSQSSPLSVSHQPSNGGLSPGNENGQVSGSLSSGASRITDDATSA 240

Query: 241  SSRGSGMMNGNSILWKSGLEQLGMNLGFSEGGGVAFVRQQVALFVDESIENLEKQEIAFK 300
            SSRGSGMMNGNSILWKSGL+QLGMNLG SE GGVAFVRQQVA F DESIENLEKQEIAFK
Sbjct: 241  SSRGSGMMNGNSILWKSGLDQLGMNLGTSE-GGVAFVRQQVAFFEDESIENLEKQEIAFK 300

Query: 301  LMTHILDNSSFDARLWEQMRALAKKQLQTLPTFLKIKKRDWSEQGSVLKARINTKLLVYQ 360
            LMTHILDNSSFDARLWEQMRALAKKQLQTLPTFLKIKKRDWSEQGSVLKARINTKLLVYQ
Sbjct: 301  LMTHILDNSSFDARLWEQMRALAKKQLQTLPTFLKIKKRDWSEQGSVLKARINTKLLVYQ 360

Query: 361  AAARLKMKTVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFSSLLTGL 420
            AAARLKM TVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELF SLLTGL
Sbjct: 361  AAARLKMTTVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFGSLLTGL 420

Query: 421  AQIAVARGGQPLRVLLIRLKPLVLTVCVQADTWGTNQGAMFESVLTTCCEIIESCWTKDR 480
            AQIAV RGGQPLRVLLIRLKPL LTVC QADTWG+NQGAMFESVLTTCCEIIESCWTKDR
Sbjct: 421  AQIAVTRGGQPLRVLLIRLKPLALTVCAQADTWGSNQGAMFESVLTTCCEIIESCWTKDR 480

Query: 481  APVDTFIMGLATSIRDRNDSEEQDDKEKQGVPMQLNVIRLLAKMTVAVNKSEIVDMILPL 540
            APVDTFIMGLATSIRDRNDSEEQDDKEKQ VP QLNVIRLLAKM+ AVNKSEIV+MILPL
Sbjct: 481  APVDTFIMGLATSIRDRNDSEEQDDKEKQAVPTQLNVIRLLAKMSAAVNKSEIVEMILPL 540

Query: 541  FIESLEEGDASTPGLLRLQLLDAVSRMATLGFEKSYRETIVLMTRSYLSKLSSIGSSESR 600
            FIESLEEGDASTPGLLRLQLLDAVSRMA+LGFEKSYRETIVL+TRSYLSKLSSIGSSESR
Sbjct: 541  FIESLEEGDASTPGLLRLQLLDAVSRMASLGFEKSYRETIVLLTRSYLSKLSSIGSSESR 600

Query: 601  TVAPEATTERVEILPAGFLHIASGLKSAKLRLEYRHRLLSLCSDVGLAAESKSGRSGANF 660
            TVAPEATTERVEILPAGFLHIASGLKSAKLRLEYRHRLL LCSDVGLAAESKSGRSGA+F
Sbjct: 601  TVAPEATTERVEILPAGFLHIASGLKSAKLRLEYRHRLLCLCSDVGLAAESKSGRSGADF 660

Query: 661  LGPLLPAVAEICSDFDPTMDIEPSLLKLFRNLWFYIALFGLAPPIQNSHLQTKSVSTMLN 720
            LGPLLPAVAEICSDFDPTM+IEPSLLKLFRNLWFYIALFGLAPPIQ S LQTKSVS+MLN
Sbjct: 661  LGPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQKSQLQTKSVSSMLN 720

Query: 721  SVGSTAIALQAVSGPYLWNTQWSSAVQLIARGTPPLVVSSVKWLEDELELNALHNPGSRR 780
            SVGSTAIALQAVSGPYLWNTQWSSAVQ IA+GTPPLVVSSVKWLEDELELNALHNPGSRR
Sbjct: 721  SVGSTAIALQAVSGPYLWNTQWSSAVQFIAQGTPPLVVSSVKWLEDELELNALHNPGSRR 780

Query: 781  GSGNEKAALAQRAALSSALGGRVDVAAMSTISGVKATYLLAVSFLEIIRFSSNGGILNGG 840
            GSGNEKAA+AQRAALS+ALGGRVDVAAMSTISGVKATYLLAVSFLEIIRFSSNGGILNGG
Sbjct: 781  GSGNEKAAVAQRAALSAALGGRVDVAAMSTISGVKATYLLAVSFLEIIRFSSNGGILNGG 840

Query: 841  SNVNASRSAFCCVFEYLKTPNLLPAVSQCLTAIVHRAFETAVLWLEDRISDTGNEAEVRD 900
            SNVNA+RSAF CVFEYLKTPNLLPAV+QCLTAIVHRAFETAV WLEDRIS+TGNEAEVRD
Sbjct: 841  SNVNAARSAFSCVFEYLKTPNLLPAVAQCLTAIVHRAFETAVSWLEDRISETGNEAEVRD 900

Query: 901  STLFAHTCYLIKSMSQRDEHVRDIAVNLLTQLRDKFPQVMWNSSCLDSLLFSMHNDAPST 960
            STL+AHTCYLIK MSQRDEHVRDI+VNLLTQLRDKFPQVMWNS+CLDSLLFSMHNDA ST
Sbjct: 901  STLYAHTCYLIKCMSQRDEHVRDISVNLLTQLRDKFPQVMWNSACLDSLLFSMHNDASST 960

Query: 961  VVTDPAWVVTVRSLYQRVVREWIVKSLSYAPCTCQGLLQEKLCKANTWQRAQHTPDVICL 1020
            VV+DP+WVVTVRSLYQRVVREWIVKSLSYAPCTCQGLLQEKLCKANTWQRAQHTPDVI L
Sbjct: 961  VVSDPSWVVTVRSLYQRVVREWIVKSLSYAPCTCQGLLQEKLCKANTWQRAQHTPDVISL 1020

Query: 1021 LSEIRIGTSKNEHWTGIQTANIPAVIAAAAAASGADLKLTEAFNLEVLSTGMVSATVKCN 1080
            LSEIRIGT KN++WTGIQTANIPAVI AAAAASGADLKLTEAFNLEVLSTGMVSATVKCN
Sbjct: 1021 LSEIRIGTGKNDYWTGIQTANIPAVITAAAAASGADLKLTEAFNLEVLSTGMVSATVKCN 1080

Query: 1081 HAGEIAGMRRLYNSIGGFQTGV---AGLGFGQGLQRLITGALPQQPQNEDDSFNGILIMK 1140
            HAGEIAGMRRLYNSIGGFQTGV    GLGFGQGLQRLITGALPQQ QNEDDSFNGILIMK
Sbjct: 1081 HAGEIAGMRRLYNSIGGFQTGVTGPTGLGFGQGLQRLITGALPQQSQNEDDSFNGILIMK 1140

Query: 1141 FVQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLASESKTNIEGFAQLIRLLCWCP 1200
            FVQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLASE+KTNIEGFAQLIRLLCWCP
Sbjct: 1141 FVQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLASEAKTNIEGFAQLIRLLCWCP 1200

Query: 1201 AYISTPDAIETGVFVWTWLVSAAPELGSFVLAELVDAWLWTVDTKRGLFASDVKYSGPAA 1260
            AYISTPDAIETGVF+WTWLVSAAPELGSFVLAELVDAWLWT+DTKRGLFAS+VKYSGPAA
Sbjct: 1201 AYISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRGLFASEVKYSGPAA 1260

Query: 1261 MLRPHLSPGEPEMQPEIDPVEQIIAHKIWLGFFIDRFEVVRHNSVEQLLLFGRLLQGSTK 1320
             LRPHLSPGEPE QPEIDPVEQIIAH+IWLGFFIDRFEVVRHNSVEQLLLFGRLLQGSTK
Sbjct: 1261 KLRPHLSPGEPESQPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQLLLFGRLLQGSTK 1320

Query: 1321 PPWNFSRHPAATGSFFTLMLLGLKFCSCQAQGNLQNFKTGLELLEDRIYRASLGWFAHEP 1380
            PPWNFS HPAATGSFFTLMLLGLKF SCQ+QGNLQNFKTGLELLEDRIYRASLGWFAHEP
Sbjct: 1321 PPWNFSHHPAATGSFFTLMLLGLKFYSCQSQGNLQNFKTGLELLEDRIYRASLGWFAHEP 1380

Query: 1381 EWYDVKHVNFAQSEAQSVSIFLHYLSSERGNSLHSDAKMRGRENGISLIDLNDHYHPVWG 1440
            EWYDVKHVNFAQSEAQSVSIFLHYLSSERGNSL SDAK RGRENGISL+D ND YHPVWG
Sbjct: 1381 EWYDVKHVNFAQSEAQSVSIFLHYLSSERGNSLQSDAKTRGRENGISLVDSNDQYHPVWG 1440

Query: 1441 HLENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTPSRPKLTAEKWIEHARTAFSV 1500
            HLENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTP+RPKL+AEKWIEHARTAFSV
Sbjct: 1441 HLENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTPARPKLSAEKWIEHARTAFSV 1500

Query: 1501 DPRIAFSMVSRFPTNAFLRVEMNQLVQLHILDIRSIPEALPYFVTPKAVDENSELLRQLP 1560
            DPRIAFSMVSRFPTN FLR E+ QLVQLHILDIRSIPEALPYF+TPKAVDENSELLRQLP
Sbjct: 1501 DPRIAFSMVSRFPTNVFLRAEVKQLVQLHILDIRSIPEALPYFITPKAVDENSELLRQLP 1560

Query: 1561 HWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQALRYDEGRLVE 1620
            HWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPE VTFFMPQLVQALRYDEGRLVE
Sbjct: 1561 HWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPENVTFFMPQLVQALRYDEGRLVE 1620

Query: 1621 GYLLRAAKRSDIFAHILIWHLQGETSLPDSGKDVNSGKNGSFLALLPVVRQRIIDGFTPK 1680
            GYLLRAA+RSDIFAHILIWHLQGETS+PDSGKD NSGKN SFLALLP+VR  II+GFTPK
Sbjct: 1621 GYLLRAAQRSDIFAHILIWHLQGETSVPDSGKDANSGKNASFLALLPIVRHHIINGFTPK 1680

Query: 1681 ALDLFKREFDFFDKVTSISGVLFPLPKDERRAGIRSELEKIEMEGEDLYLPTATNKLVCG 1740
            A DLF+REFDFFDKVTSISGVLFPLPKDERRAGIR ELEKIEMEGEDLYLPTATNKLV G
Sbjct: 1681 ARDLFEREFDFFDKVTSISGVLFPLPKDERRAGIRRELEKIEMEGEDLYLPTATNKLVRG 1740

Query: 1741 IQVDSGIPLQSAAKVPIMVTFNVVDRDGDPNNIKPQACIFKVGDDCRQDVLALQVISLLR 1800
            IQVDSGIPLQSAAKVPIM+TFNVVDRDGDPNNIKPQACIFKVGDDCRQDVLALQVI+LLR
Sbjct: 1741 IQVDSGIPLQSAAKVPIMITFNVVDRDGDPNNIKPQACIFKVGDDCRQDVLALQVIALLR 1800

Query: 1801 DIFQAVGLNLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGPVGS 1860
            DIFQAVGLNLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGPVGS
Sbjct: 1801 DIFQAVGLNLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGPVGS 1860

Query: 1861 PNFEAARENFIVSSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFILETSPGGNMR 1920
            P+FEAARENFI+SSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFILETSPGGNMR
Sbjct: 1861 PSFEAARENFIISSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFILETSPGGNMR 1920

Query: 1921 FESAHFKLSHEMTQLLDPSGVMKSETWNLFVRLCVKGYLAARRHMHGIINTVLLMLDSGL 1980
            FESAHFKLSHEMTQLLDPSGVMKSETWNLFV LCVKGYLAARRHM GIINTVLLMLDSGL
Sbjct: 1921 FESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMDGIINTVLLMLDSGL 1980

Query: 1981 PCFSRGDPIGNLRKRFHPEMNDREAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK 2035
            PCFSRGDPIGNLRKRFHPEM++REAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK
Sbjct: 1981 PCFSRGDPIGNLRKRFHPEMSEREAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK 2036

BLAST of Chy1G008030 vs. ExPASy TrEMBL
Match: A0A6J1GKT2 (phosphatidylinositol 4-kinase alpha 1-like OS=Cucurbita moschata OX=3662 GN=LOC111454915 PE=4 SV=1)

HSP 1 Score: 3830.4 bits (9932), Expect = 0.0e+00
Identity = 1932/2037 (94.85%), Postives = 1980/2037 (97.20%), Query Frame = 0

Query: 1    MDALIELCDLIAQNPALFSEKLSWICSRCPPSEAILAGSPAISRSQLNAVLAVARLLSKC 60
            MDALIELCDLIAQNPALFSEKLSWICSRCPPSEAILAGSPAISRSQLNAVLAVARLLSKC
Sbjct: 1    MDALIELCDLIAQNPALFSEKLSWICSRCPPSEAILAGSPAISRSQLNAVLAVARLLSKC 60

Query: 61   PDSVGLRPKSVVLEFLRSIPLSFSLSFWPQSYGNDAIASFFNEFLNYTSKACALSTDFAT 120
            PDSVGLRPKSVVLEFLRSIPLSFSLSFWPQS+GNDAIASFF EFLNYTS+AC LSTDFA 
Sbjct: 61   PDSVGLRPKSVVLEFLRSIPLSFSLSFWPQSFGNDAIASFFTEFLNYTSQACELSTDFAM 120

Query: 121  EVSGFSSEVVLSAINDCSEGSAISRAFLVALSKSFLPIIPSDADKLVSCILDRFLISEAA 180
            EVSGF+SEVVLSAI+DCSEGSAIS AFLVALSKSFLPIIPSDADKLVSCILD FLISE  
Sbjct: 121  EVSGFTSEVVLSAISDCSEGSAISSAFLVALSKSFLPIIPSDADKLVSCILDLFLISEVV 180

Query: 181  PGTPREHNQANSEPSSSQSSPLSVSHQPSNGGLSPGNENGQVSGSLSSGASRITDDATSA 240
            PGTPRE  QANSEPSSSQSSPLSVSHQP NGGLSPGNENGQVSGSLSSGASRITDDATSA
Sbjct: 181  PGTPREQIQANSEPSSSQSSPLSVSHQPLNGGLSPGNENGQVSGSLSSGASRITDDATSA 240

Query: 241  SSRGSGMMNGNSILWKSGLEQLGMNLGFSEGGGVAFVRQQVALFVDESIENLEKQEIAFK 300
            SSRGSGMMNGNSILWKSGL+QLGMNLG SE GG+AFVRQQVA F DESIENLEKQEIAFK
Sbjct: 241  SSRGSGMMNGNSILWKSGLDQLGMNLGTSE-GGLAFVRQQVAFFEDESIENLEKQEIAFK 300

Query: 301  LMTHILDNSSFDARLWEQMRALAKKQLQTLPTFLKIKKRDWSEQGSVLKARINTKLLVYQ 360
            LMTHILDNSSFDARLWEQMRALAKKQLQTLPTFLKIKKRDWSEQGSVLKARINTKLLVYQ
Sbjct: 301  LMTHILDNSSFDARLWEQMRALAKKQLQTLPTFLKIKKRDWSEQGSVLKARINTKLLVYQ 360

Query: 361  AAARLKMKTVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFSSLLTGL 420
            AAARLKM TVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELF SLLTGL
Sbjct: 361  AAARLKMTTVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFGSLLTGL 420

Query: 421  AQIAVARGGQPLRVLLIRLKPLVLTVCVQADTWGTNQGAMFESVLTTCCEIIESCWTKDR 480
            AQIAV RGGQPLRVLLIRLKPL LTVC QADTWG+NQGAMFESVLTTCCEIIESCWTKDR
Sbjct: 421  AQIAVTRGGQPLRVLLIRLKPLALTVCAQADTWGSNQGAMFESVLTTCCEIIESCWTKDR 480

Query: 481  APVDTFIMGLATSIRDRNDSEEQDDKEKQGVPMQLNVIRLLAKMTVAVNKSEIVDMILPL 540
            APVDTFIMGLATSIRDRNDSEEQDDKEKQ VPMQLNVIRLLAKM+ AVNKSEIV+MILPL
Sbjct: 481  APVDTFIMGLATSIRDRNDSEEQDDKEKQAVPMQLNVIRLLAKMSAAVNKSEIVEMILPL 540

Query: 541  FIESLEEGDASTPGLLRLQLLDAVSRMATLGFEKSYRETIVLMTRSYLSKLSSIGSSESR 600
            FIESLEEGDASTPGLLRLQLLDAVSRMA+LGFEKSYRETIVL+TRSYLSKLSSIGSSESR
Sbjct: 541  FIESLEEGDASTPGLLRLQLLDAVSRMASLGFEKSYRETIVLLTRSYLSKLSSIGSSESR 600

Query: 601  TVAPEATTERVEILPAGFLHIASGLKSAKLRLEYRHRLLSLCSDVGLAAESKSGRSGANF 660
            TVAPEATTERVEILPAGFLHIASGLKSAKLRLEYRHRLL LCSDVGLAAESKSGRSGA+F
Sbjct: 601  TVAPEATTERVEILPAGFLHIASGLKSAKLRLEYRHRLLCLCSDVGLAAESKSGRSGADF 660

Query: 661  LGPLLPAVAEICSDFDPTMDIEPSLLKLFRNLWFYIALFGLAPPIQNSHLQTKSVSTMLN 720
            LGPLLPAVAEICSDFDPTM+IEPSLLKLFRNLWFYIALFGLAPPIQ S LQTKSVS+MLN
Sbjct: 661  LGPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQKSQLQTKSVSSMLN 720

Query: 721  SVGSTAIALQAVSGPYLWNTQWSSAVQLIARGTPPLVVSSVKWLEDELELNALHNPGSRR 780
            SVGSTAIALQAVSGPYLWNTQWSSAVQ IA+GTPPLVVSSVKWLEDELELNALHNPGSRR
Sbjct: 721  SVGSTAIALQAVSGPYLWNTQWSSAVQFIAQGTPPLVVSSVKWLEDELELNALHNPGSRR 780

Query: 781  GSGNEKAALAQRAALSSALGGRVDVAAMSTISGVKATYLLAVSFLEIIRFSSNGGILNGG 840
            GSGNEKAA+AQRAALS+ALGGRVDVAAMSTISGVKATYLLAVSFLEIIRFSSNGGILNGG
Sbjct: 781  GSGNEKAAVAQRAALSAALGGRVDVAAMSTISGVKATYLLAVSFLEIIRFSSNGGILNGG 840

Query: 841  SNVNASRSAFCCVFEYLKTPNLLPAVSQCLTAIVHRAFETAVLWLEDRISDTGNEAEVRD 900
            SNVNA+RSAF CVFEYLKTPNLLPAV+QCLTAIVHRAFETAV WLEDRIS+TGNEAEVRD
Sbjct: 841  SNVNAARSAFSCVFEYLKTPNLLPAVAQCLTAIVHRAFETAVSWLEDRISETGNEAEVRD 900

Query: 901  STLFAHTCYLIKSMSQRDEHVRDIAVNLLTQLRDKFPQVMWNSSCLDSLLFSMHNDAPST 960
            STL+AHTCYLIK MSQRDEHVRDI+VNLLTQLRDKFPQVMWNS+CLDSLLFSMHNDA ST
Sbjct: 901  STLYAHTCYLIKCMSQRDEHVRDISVNLLTQLRDKFPQVMWNSACLDSLLFSMHNDASST 960

Query: 961  VVTDPAWVVTVRSLYQRVVREWIVKSLSYAPCTCQGLLQEKLCKANTWQRAQHTPDVICL 1020
            VV+DPAWVVTVRSLYQRVVREWIVKSLSYAPCTCQGLLQEKLCKANTWQRAQHTPDVI L
Sbjct: 961  VVSDPAWVVTVRSLYQRVVREWIVKSLSYAPCTCQGLLQEKLCKANTWQRAQHTPDVISL 1020

Query: 1021 LSEIRIGTSKNEHWTGIQTANIPAVIAAAAAASGADLKLTEAFNLEVLSTGMVSATVKCN 1080
            LSEIRIGT KN++WTGIQTANIPAVI AAAAASGADLKLTEAFNLEVLSTGMVSATVKCN
Sbjct: 1021 LSEIRIGTGKNDYWTGIQTANIPAVITAAAAASGADLKLTEAFNLEVLSTGMVSATVKCN 1080

Query: 1081 HAGEIAGMRRLYNSIGGFQTGVA---GLGFGQGLQRLITGALPQQPQNEDDSFNGILIMK 1140
            HAGEIAGMRRLYNSIGGFQTGVA   GLGFGQGLQRLITG LPQQPQNEDDSFNGILIMK
Sbjct: 1081 HAGEIAGMRRLYNSIGGFQTGVAGPTGLGFGQGLQRLITGTLPQQPQNEDDSFNGILIMK 1140

Query: 1141 FVQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLASESKTNIEGFAQLIRLLCWCP 1200
            FVQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLASESKTNIEGFAQLIRLLCWCP
Sbjct: 1141 FVQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLASESKTNIEGFAQLIRLLCWCP 1200

Query: 1201 AYISTPDAIETGVFVWTWLVSAAPELGSFVLAELVDAWLWTVDTKRGLFASDVKYSGPAA 1260
            AYISTPDAIETGVF+WTWLVSAAPELGSFVLAELVDAWLWT+DTKRGLFAS+VKYSGPAA
Sbjct: 1201 AYISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRGLFASEVKYSGPAA 1260

Query: 1261 MLRPHLSPGEPEMQPEIDPVEQIIAHKIWLGFFIDRFEVVRHNSVEQLLLFGRLLQGSTK 1320
             LRPHLSPGEPE QPEIDPVEQIIAH+IWLGFFIDRFEVVRHNSVEQLLLFGRLLQGSTK
Sbjct: 1261 KLRPHLSPGEPESQPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQLLLFGRLLQGSTK 1320

Query: 1321 PPWNFSRHPAATGSFFTLMLLGLKFCSCQAQGNLQNFKTGLELLEDRIYRASLGWFAHEP 1380
            PPWNFSRHPAATGSFFTLMLLGLKF SCQ+QGNLQNFKTGLELLEDRIYRASLGWFAHEP
Sbjct: 1321 PPWNFSRHPAATGSFFTLMLLGLKFYSCQSQGNLQNFKTGLELLEDRIYRASLGWFAHEP 1380

Query: 1381 EWYDVKHVNFAQSEAQSVSIFLHYLSSERGNSLHSDAKMRGRENGISLIDLNDHYHPVWG 1440
            EWYDVKHVNFAQSEAQSVSIFLHYLSSERGNSL SD K RGRENGISL+D ND YHPVWG
Sbjct: 1381 EWYDVKHVNFAQSEAQSVSIFLHYLSSERGNSLQSDTKTRGRENGISLVDSNDQYHPVWG 1440

Query: 1441 HLENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTPSRPKLTAEKWIEHARTAFSV 1500
            HLENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTP+RPKL+AEKWIEHARTAFSV
Sbjct: 1441 HLENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTPARPKLSAEKWIEHARTAFSV 1500

Query: 1501 DPRIAFSMVSRFPTNAFLRVEMNQLVQLHILDIRSIPEALPYFVTPKAVDENSELLRQLP 1560
            DPRIAFSMVSRFPTN FLR E+ QLVQLHILDIRSIPEALPYF+TPKAV+ENSELLRQLP
Sbjct: 1501 DPRIAFSMVSRFPTNGFLRAEVKQLVQLHILDIRSIPEALPYFITPKAVEENSELLRQLP 1560

Query: 1561 HWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQALRYDEGRLVE 1620
            HWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPE VTFFMPQLVQALRYDEGRLVE
Sbjct: 1561 HWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPENVTFFMPQLVQALRYDEGRLVE 1620

Query: 1621 GYLLRAAKRSDIFAHILIWHLQGETSLPDSGKDVNSGKNGSFLALLPVVRQRIIDGFTPK 1680
            GYLLRAA+RSDIFAHILIWHLQGETS+PDSGKD NSGKN SFLALLP+VR  IIDGFTPK
Sbjct: 1621 GYLLRAAQRSDIFAHILIWHLQGETSVPDSGKDANSGKNASFLALLPIVRHHIIDGFTPK 1680

Query: 1681 ALDLFKREFDFFDKVTSISGVLFPLPKDERRAGIRSELEKIEMEGEDLYLPTATNKLVCG 1740
            A DLF+REFDFFDKVTSISGVLFPLPKDERRAGIR ELEKIEMEGEDLYLPTATNKLV G
Sbjct: 1681 ARDLFEREFDFFDKVTSISGVLFPLPKDERRAGIRRELEKIEMEGEDLYLPTATNKLVRG 1740

Query: 1741 IQVDSGIPLQSAAKVPIMVTFNVVDRDGDPNNIKPQACIFKVGDDCRQDVLALQVISLLR 1800
            IQVDSGIPLQSAAKVPIM+TFNVVDRDGDPNNIKPQACIFKVGDDCRQDVLALQVI+LLR
Sbjct: 1741 IQVDSGIPLQSAAKVPIMITFNVVDRDGDPNNIKPQACIFKVGDDCRQDVLALQVIALLR 1800

Query: 1801 DIFQAVGLNLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGPVGS 1860
            DIFQAVGLNLYLFPYGVLPTGPG+GIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGPVGS
Sbjct: 1801 DIFQAVGLNLYLFPYGVLPTGPGKGIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGPVGS 1860

Query: 1861 PNFEAARENFIVSSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFILETSPGGNMR 1920
            P+FEAARENFI+SSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFILETSPGGNMR
Sbjct: 1861 PSFEAARENFIISSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFILETSPGGNMR 1920

Query: 1921 FESAHFKLSHEMTQLLDPSGVMKSETWNLFVRLCVKGYLAARRHMHGIINTVLLMLDSGL 1980
            FESAHFKLSHEMTQLLDPSGVMKSETWNLFV LCVKGYLAARRHM GIINTVLLMLDSGL
Sbjct: 1921 FESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMDGIINTVLLMLDSGL 1980

Query: 1981 PCFSRGDPIGNLRKRFHPEMNDREAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK 2035
            PCFSRGDPIGNLRKRFHPEM++REAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK
Sbjct: 1981 PCFSRGDPIGNLRKRFHPEMSEREAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK 2036

BLAST of Chy1G008030 vs. ExPASy TrEMBL
Match: A0A6J1C3T4 (phosphatidylinositol 4-kinase alpha 1-like OS=Momordica charantia OX=3673 GN=LOC111007670 PE=4 SV=1)

HSP 1 Score: 3730.6 bits (9673), Expect = 0.0e+00
Identity = 1883/2047 (91.99%), Postives = 1954/2047 (95.46%), Query Frame = 0

Query: 1    MDALIELCDLIAQNPALFSEKLSWICSRCPPSEAILAGSPAISRSQLNAVLAVARLLSKC 60
            M+ALIELCDLIAQNPALFSEKLSWICSRCPPSE  LAGSPAISRSQLNAVLAVARLLSKC
Sbjct: 1    MEALIELCDLIAQNPALFSEKLSWICSRCPPSEVFLAGSPAISRSQLNAVLAVARLLSKC 60

Query: 61   PDSVGLRPKSVVLEFLRSIPLSFSLSFWPQSYGNDAIASFFNEFLNYTSKACALSTDFAT 120
            PDSVGLRPKSVV+EF+RSIPLSF+LSFWPQSYGNDAIASF+ EF+NYTSKAC LST+FAT
Sbjct: 61   PDSVGLRPKSVVIEFIRSIPLSFNLSFWPQSYGNDAIASFYAEFMNYTSKACELSTEFAT 120

Query: 121  EVSGFSSEVVLSAINDCSEGSAISRAFLVALSKSFLPIIPSDADKLVSCILDRFLISEAA 180
            E+SGF+SEVVLSAI+DCSEGS ISRAFLVA+SKSF+PI+PSDADKLVSCILDRFLISEAA
Sbjct: 121  EISGFTSEVVLSAIDDCSEGSPISRAFLVAMSKSFIPILPSDADKLVSCILDRFLISEAA 180

Query: 181  PGTPREHNQANSEPSSSQSSPLSVSHQPSNGGLSPGNENGQVSGSLSSGASRITDDATSA 240
            PGTPRE  +ANSE SSSQ SPLSVSH P NGGLSPGNENGQVSGSLSSG SRITDDATSA
Sbjct: 181  PGTPREQIRANSEASSSQGSPLSVSHHPLNGGLSPGNENGQVSGSLSSGGSRITDDATSA 240

Query: 241  SSRGSGMMNGNSILWKSGLEQLGMNLGFSEGGGVAFVRQQVALFVDESIENLEKQEIAFK 300
            SSRGSGMMNG+S+LWKSG++QLGMNLG SEGGGVA VRQQV +F DESIENLEKQEIAFK
Sbjct: 241  SSRGSGMMNGSSLLWKSGVDQLGMNLGVSEGGGVASVRQQVTVFEDESIENLEKQEIAFK 300

Query: 301  LMTHILDNSSFDARLWEQMRALAKKQLQTLPTFLKIKKRDWSEQGSVLKARINTKLLVYQ 360
            L+THILDNSSFDARLWEQMRAL KKQLQTLPTFLKIKKRDWSEQGSVLKARINTKLLVYQ
Sbjct: 301  LVTHILDNSSFDARLWEQMRALLKKQLQTLPTFLKIKKRDWSEQGSVLKARINTKLLVYQ 360

Query: 361  AAARLKMKTVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFSSLLTGL 420
            AAARLKMK VASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELF SLLTGL
Sbjct: 361  AAARLKMKAVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFGSLLTGL 420

Query: 421  AQIAVARGGQPLRVLLIRLKPLVLTVCVQADTWGTNQGAMFESVLTTCCEIIESCWTKDR 480
            AQIAVARGGQPLRVLLIRLKPLVLTVC QADTWG++QGAMFESVL TCCEIIESCWTKDR
Sbjct: 421  AQIAVARGGQPLRVLLIRLKPLVLTVCAQADTWGSSQGAMFESVLKTCCEIIESCWTKDR 480

Query: 481  APVDTFIMGLATSIRDRNDSEEQDDKEKQGVPMQLNVIRLLAKMTVAVNKSEIVDMILPL 540
            APVDTFIMGLATSIRDRNDSEEQDDKEKQ VPMQLNVIRLLAKMTVAVNKSE+VDMILPL
Sbjct: 481  APVDTFIMGLATSIRDRNDSEEQDDKEKQAVPMQLNVIRLLAKMTVAVNKSEVVDMILPL 540

Query: 541  FIESLEEGDASTPGLLRLQLLDAVSRMATLGFEKSYRETIVLMTRSYLSKLSSIGSSESR 600
            FIESLEEGDASTPGLLRLQLLDAVSRMA+LGFEKSYRETIVLMTRSYLSKLSSIGSSESR
Sbjct: 541  FIESLEEGDASTPGLLRLQLLDAVSRMASLGFEKSYRETIVLMTRSYLSKLSSIGSSESR 600

Query: 601  TVAPEATTERVEILPAGFLHIASGLKSAKLRLEYRHRLLSLCSDVGLAAESKSGRSGANF 660
            T+APEA TERVE LPAGFL IASGLK AKLR EYRHRLLSLCSDVGL+AESKSGRSGA+F
Sbjct: 601  TLAPEANTERVETLPAGFLLIASGLKGAKLRSEYRHRLLSLCSDVGLSAESKSGRSGADF 660

Query: 661  LGPLLPAVAEICSDFDPTMDIEPSLLKLFRNLWFYIALFGLAPPIQNSHLQTKSVSTMLN 720
            LGPLLPAVAEICSDFDPTM+IEPSLLKLFRNLWFYIALFGLAPPIQ S LQTKSVS+MLN
Sbjct: 661  LGPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQKSQLQTKSVSSMLN 720

Query: 721  SVGST-AIALQAVSGPYLWNTQWSSAVQLIARGTPPLVVSSVKWLEDELELNALHNPGSR 780
            SVGS  AIALQAVSGPY+WNTQWSSAVQ IA+GTPPLVVSSVKWLEDELELNALHNPGSR
Sbjct: 721  SVGSMGAIALQAVSGPYMWNTQWSSAVQFIAQGTPPLVVSSVKWLEDELELNALHNPGSR 780

Query: 781  RGSGNEKAALAQR----AALSSALGGRVDVAAMSTIS-----GVKATYLLAVSFLEIIRF 840
            +GSGNEKAAL+        L       V     S IS     GVKATYLLAVSFLEIIRF
Sbjct: 781  QGSGNEKAALSVAYYIVYCLDVFFSRMVFSFLPSQISLFHFVGVKATYLLAVSFLEIIRF 840

Query: 841  SSNGGILNGGSNVNASRSAFCCVFEYLKTPNLLPAVSQCLTAIVHRAFETAVLWLEDRIS 900
            SSNGG+LNGGSN+NASRSAF CVFEYLKTPNLLPAVSQCLTA+VH AFETAV WL+DRIS
Sbjct: 841  SSNGGVLNGGSNLNASRSAFSCVFEYLKTPNLLPAVSQCLTAMVHGAFETAVSWLDDRIS 900

Query: 901  DTGNEAEVRDSTLFAHTCYLIKSMSQRDEHVRDIAVNLLTQLRDKFPQVMWNSSCLDSLL 960
            +TGNEAEVRDSTLFAHTC+LI+SMSQRDEHVRDIAVNLLTQLRDKFPQVMWNSSCLDSLL
Sbjct: 901  ETGNEAEVRDSTLFAHTCFLIRSMSQRDEHVRDIAVNLLTQLRDKFPQVMWNSSCLDSLL 960

Query: 961  FSMHNDAPSTVVTDPAWVVTVRSLYQRVVREWIVKSLSYAPCTCQGLLQEKLCKANTWQR 1020
            FSMHNDAPS VVTDPAWVVTVRSLYQ VVREWI+KSLSYAPCT QGLLQEKLCK NTWQR
Sbjct: 961  FSMHNDAPSAVVTDPAWVVTVRSLYQGVVREWIIKSLSYAPCTSQGLLQEKLCKVNTWQR 1020

Query: 1021 AQHTPDVICLLSEIRIGTSKNEHWTGIQTANIPAVIAAAAAASGADLKLTEAFNLEVLST 1080
            AQHTPDVI LLSEIRIGT KN+ WTGIQTANIPAV+ AAAAASGADLKLTEAFNLEVL T
Sbjct: 1021 AQHTPDVISLLSEIRIGTGKNDCWTGIQTANIPAVMTAAAAASGADLKLTEAFNLEVLGT 1080

Query: 1081 GMVSATVKCNHAGEIAGMRRLYNSIGGFQTGVA---GLGFGQGLQRLITGALPQQPQNED 1140
            GMVSATVKCNHAGEIAGMRRLYNSIGGFQTGVA   GLGFGQG QRLI+GA PQ+PQNED
Sbjct: 1081 GMVSATVKCNHAGEIAGMRRLYNSIGGFQTGVAAPTGLGFGQGFQRLISGAFPQKPQNED 1140

Query: 1141 DSFNGILIMKFVQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLASESKTNIEGFA 1200
            DSFNGILIMKFVQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLASESKTNIEGFA
Sbjct: 1141 DSFNGILIMKFVQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLASESKTNIEGFA 1200

Query: 1201 QLIRLLCWCPAYISTPDAIETGVFVWTWLVSAAPELGSFVLAELVDAWLWTVDTKRGLFA 1260
            +LIRLLCWCPAYISTPDAIETGVF+WTWLVSAAPELGSFVLAELVDAWLWT+DTKRGLFA
Sbjct: 1201 RLIRLLCWCPAYISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRGLFA 1260

Query: 1261 SDVKYSGPAAMLRPHLSPGEPEMQPEIDPVEQIIAHKIWLGFFIDRFEVVRHNSVEQLLL 1320
            S+VKYSGPAA LRPHLSPGEPE+QPEIDPVEQIIAH+IWLGFFIDRFEVVRHNSVEQ+LL
Sbjct: 1261 SEVKYSGPAAKLRPHLSPGEPEVQPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQVLL 1320

Query: 1321 FGRLLQGSTKPPWNFSRHPAATGSFFTLMLLGLKFCSCQAQGNLQNFKTGLELLEDRIYR 1380
            FGRLLQGSTKPPWNFSRHPAATGSFFTLMLLGLKFCSCQ+QGNLQNFKTGLELLEDRIYR
Sbjct: 1321 FGRLLQGSTKPPWNFSRHPAATGSFFTLMLLGLKFCSCQSQGNLQNFKTGLELLEDRIYR 1380

Query: 1381 ASLGWFAHEPEWYDVKHVNFAQSEAQSVSIFLHYLSSERGNSLHSDAKMRGRENGISLID 1440
            ASLGWFAHEPEWYDVKHVNFAQSEAQSVSIFLHYLSS+RGN L  DAK RGRENGIS+++
Sbjct: 1381 ASLGWFAHEPEWYDVKHVNFAQSEAQSVSIFLHYLSSDRGNGLQFDAKTRGRENGISVVN 1440

Query: 1441 LNDHYHPVWGHLENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTPSRPKLTAEKW 1500
              D YHPVWGHLENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTPSRPKL++EKW
Sbjct: 1441 SKDQYHPVWGHLENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTPSRPKLSSEKW 1500

Query: 1501 IEHARTAFSVDPRIAFSMVSRFPTNAFLRVEMNQLVQLHILDIRSIPEALPYFVTPKAVD 1560
            IEH RTAFSVDPRIAFSMVSRFPTNA LRVEMNQLVQLHILDIRSIPEALPYFVTPKAVD
Sbjct: 1501 IEHVRTAFSVDPRIAFSMVSRFPTNASLRVEMNQLVQLHILDIRSIPEALPYFVTPKAVD 1560

Query: 1561 ENSELLRQLPHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQA 1620
            ENSELLRQLPHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQA
Sbjct: 1561 ENSELLRQLPHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQA 1620

Query: 1621 LRYDEGRLVEGYLLRAAKRSDIFAHILIWHLQGETSLPDSGKDVNSGKNGSFLALLPVVR 1680
            LRYDEGRLVEGYLLRAA+RSDIFAHILIWHLQGETS+PDSGKD NSGKNGSFLALLPVVR
Sbjct: 1621 LRYDEGRLVEGYLLRAAQRSDIFAHILIWHLQGETSVPDSGKDANSGKNGSFLALLPVVR 1680

Query: 1681 QRIIDGFTPKALDLFKREFDFFDKVTSISGVLFPLPKDERRAGIRSELEKIEMEGEDLYL 1740
            Q IIDGFTPKALDLF+REF+FFDKVTSISGVLFPLPKDERRAGIR ELEKIEMEGEDLYL
Sbjct: 1681 QHIIDGFTPKALDLFEREFNFFDKVTSISGVLFPLPKDERRAGIRRELEKIEMEGEDLYL 1740

Query: 1741 PTATNKLVCGIQVDSGIPLQSAAKVPIMVTFNVVDRDGDPNNIKPQACIFKVGDDCRQDV 1800
            PTATNKLV GIQVDSGIPLQSAAKVPIM+TFNVV+RDGDPN+IKPQACIFKVGDDCRQDV
Sbjct: 1741 PTATNKLVRGIQVDSGIPLQSAAKVPIMITFNVVNRDGDPNDIKPQACIFKVGDDCRQDV 1800

Query: 1801 LALQVISLLRDIFQAVGLNLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLYEI 1860
            LALQVI+LLRDIFQAVG+NLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLYEI
Sbjct: 1801 LALQVIALLRDIFQAVGINLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLYEI 1860

Query: 1861 FQQDYGPVGSPNFEAARENFIVSSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFI 1920
            FQQDYGPVGSPNFEAARENFI+SSAGYAVASLLLQPKDRHNGNLL DN GRLVHIDFGFI
Sbjct: 1861 FQQDYGPVGSPNFEAARENFIISSAGYAVASLLLQPKDRHNGNLLIDNTGRLVHIDFGFI 1920

Query: 1921 LETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSETWNLFVRLCVKGYLAARRHMHGIIN 1980
            LETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSETWNLFV LCVKGYLAARRHM GIIN
Sbjct: 1921 LETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMDGIIN 1980

Query: 1981 TVLLMLDSGLPCFSRGDPIGNLRKRFHPEMNDREAANFMIRVCTDAYNKWTTAGYDLIQY 2035
            TVLLMLDSGLPCFSRGDPIGNLRKRFHPEM++REAANFMIRVCTDAYNKWTTAGYDLIQY
Sbjct: 1981 TVLLMLDSGLPCFSRGDPIGNLRKRFHPEMSEREAANFMIRVCTDAYNKWTTAGYDLIQY 2040

BLAST of Chy1G008030 vs. ExPASy TrEMBL
Match: A0A6P3YUH8 (phosphatidylinositol 4-kinase alpha 1 OS=Ziziphus jujuba OX=326968 GN=LOC107404942 PE=4 SV=1)

HSP 1 Score: 3286.1 bits (8519), Expect = 0.0e+00
Identity = 1646/2044 (80.53%), Postives = 1834/2044 (89.73%), Query Frame = 0

Query: 1    MDALIELCDLIAQNPALFSEKLSWICSRCPPSEAILAGSPAISRSQLNAVLAVARLLSKC 60
            M+ALIELCDLIAQNP  FSEKLSWIC RCPP E++L GSP +SRSQLNAVLAVAR LSKC
Sbjct: 1    MEALIELCDLIAQNPVQFSEKLSWICGRCPPPESLLEGSPRVSRSQLNAVLAVARFLSKC 60

Query: 61   PDSVGLRPKSVVLEFLRSIPLSFSLSFWPQSYGNDAIASFFNEFLNYTSKACALSTDFAT 120
             DS  LRPKSVVLEFLR IP SF+ SFWPQS+GNDAIASFF +FL Y SKA  LS DFAT
Sbjct: 61   QDSADLRPKSVVLEFLRFIPASFNQSFWPQSFGNDAIASFFADFLGYVSKAPELSPDFAT 120

Query: 121  EVSGFSSEVVLSAINDCSEGSAISRAFLVALSKSFLPIIPSDADKLVSCILDRFLIS--- 180
            E++GF+ +VV+SAI++ S    ISR FL ALS+SF PI  SDA++LV+ +LD+   S   
Sbjct: 121  EIAGFTGDVVVSAISNASADLGISRVFLTALSQSFPPISASDAERLVNLLLDQLAASGTI 180

Query: 181  -EAAPGTPREHNQANSEPSSSQSSPLSVSH-QPSNGGLSPGNENGQVSGSLSSGASRITD 240
               +P TPRE   ANSE SSSQSSPLS++H QP  G  SPGNE   VSGS  S ASRI D
Sbjct: 181  VAQSPATPREQITANSETSSSQSSPLSLNHSQPHGGSSSPGNEASNVSGSSGSAASRIAD 240

Query: 241  DATSASSRGSGMMNGNSILWKSGLEQLGMNLGFSEGGGVAFVRQQVALFVDESIENLEKQ 300
            DATSASSRGS MMNG SILWKSG++QLG+N G+++GGG   +RQQV+ F +ES+E+LEKQ
Sbjct: 241  DATSASSRGSMMMNGGSILWKSGVDQLGVNFGYNDGGGAMLLRQQVSSFEEESVESLEKQ 300

Query: 301  EIAFKLMTHILDNSSFDARLWEQMRALAKKQLQTLPTFLKIKKRDWSEQGSVLKARINTK 360
            EIAFKL+ HIL+    D  L EQ+R +AK+QLQ+L  FLKI+KRDW+E G++LKARINTK
Sbjct: 301  EIAFKLIAHILEKCCIDMGLLEQVRFIAKRQLQSLTVFLKIRKRDWNEHGTLLKARINTK 360

Query: 361  LLVYQAAARLKMKTVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFSS 420
            LLVY+AAA+L +KT+     DGK AKKL  ET AL MDAAE+CLLSVWRKLRICEELF S
Sbjct: 361  LLVYKAAAKLTLKTLDCFHSDGKLAKKLAHETLALFMDAAESCLLSVWRKLRICEELFGS 420

Query: 421  LLTGLAQIAVARGGQPLRVLLIRLKPLVLTVCVQADTWGTNQGAMFESVLTTCCEIIESC 480
            LL GL+QIAV RGGQ LR+LLIRLKP++LTVC QADTW T+QGAMFESV+ T C+IIESC
Sbjct: 421  LLAGLSQIAVHRGGQSLRILLIRLKPVILTVCTQADTWATSQGAMFESVMKTTCQIIESC 480

Query: 481  WTKDRAPVDTFIMGLATSIRDRNDSEEQDDKEKQGVP-MQLNVIRLLAKMTVAVNKSEIV 540
            W K+RAPVDTFIMGLATSIR+RND EEQ DK+K+ +P MQLNVIRLLA + VAVNKSE+V
Sbjct: 481  WAKERAPVDTFIMGLATSIRERNDYEEQVDKDKETIPVMQLNVIRLLADLNVAVNKSEVV 540

Query: 541  DMILPLFIESLEEGDASTPGLLRLQLLDAVSRMATLGFEKSYRETIVLMTRSYLSKLSSI 600
            DMILPLFIESLEEGDASTP LLRL+LLDAVSRMA+LGFEKSYRET+VLMTRSYL+KLSS+
Sbjct: 541  DMILPLFIESLEEGDASTPSLLRLRLLDAVSRMASLGFEKSYRETVVLMTRSYLNKLSSV 600

Query: 601  GSSESRTVAPEATTERVEILPAGFLHIASGLKSAKLRLEYRHRLLSLCSDVGLAAESKSG 660
            GS+ES+TVA EATTERVE LP+GF  IASGL + KLR +YRHRLLSLCSDVGLAAESKSG
Sbjct: 601  GSAESKTVAAEATTERVETLPSGFHLIASGLTNTKLRADYRHRLLSLCSDVGLAAESKSG 660

Query: 661  RSGANFLGPLLPAVAEICSDFDPTMDIEPSLLKLFRNLWFYIALFGLAPPIQNSHLQTKS 720
            RSGA+FLGPLLPAVAEICSDFDP++D+EPSLLKLFRNLWFYIALFGLAPPIQ     TK 
Sbjct: 661  RSGADFLGPLLPAVAEICSDFDPSVDVEPSLLKLFRNLWFYIALFGLAPPIQKIQHPTKP 720

Query: 721  VSTMLNSVGST-AIALQAVSGPYLWNTQWSSAVQLIARGTPPLVVSSVKWLEDELELNAL 780
            VST LNSVGS   IALQAV GPY+WNTQWSSAVQ IA+GTP LVVSSVKWLEDELELNAL
Sbjct: 721  VSTTLNSVGSMGTIALQAVGGPYMWNTQWSSAVQQIAQGTPSLVVSSVKWLEDELELNAL 780

Query: 781  HNPGSRRGSGNEKAALAQRAALSSALGGRVDVAAMSTISGVKATYLLAVSFLEIIRFSSN 840
            HNPGSRRGSGNEKAA++QRAALS+ALGGRVDV +M+TISGVKATYLLAV+FLEIIRFSSN
Sbjct: 781  HNPGSRRGSGNEKAAVSQRAALSAALGGRVDVGSMNTISGVKATYLLAVAFLEIIRFSSN 840

Query: 841  GGILNGGSNVNASRSAFCCVFEYLKTPNLLPAVSQCLTAIVHRAFETAVLWLEDRISDTG 900
            GG++NGG+++NASRSAF CVFEYLK+PNL+PAV QCL AIVHRAFETAVLWLEDRIS+TG
Sbjct: 841  GGVINGGTSLNASRSAFSCVFEYLKSPNLMPAVFQCLMAIVHRAFETAVLWLEDRISETG 900

Query: 901  NEAEVRDSTLFAHTCYLIKSMSQRDEHVRDIAVNLLTQLRDKFPQVMWNSSCLDSLLFSM 960
             +AE R+STL AH C+LIKSMSQR+EH+RD+AVNLL QLRDKFPQV+WNSSC DSLLFSM
Sbjct: 901  MKAEYRESTLSAHACFLIKSMSQREEHIRDVAVNLLAQLRDKFPQVLWNSSCFDSLLFSM 960

Query: 961  HNDAPSTVVTDPAWVVTVRSLYQRVVREWIVKSLSYAPCTCQGLLQEKLCKANTWQRAQH 1020
            HND+PS VV DP+W+VTVRSLYQ++VREWI+KSLS+APCT QGLLQ+KLCKANTWQRAQ 
Sbjct: 961  HNDSPSVVVNDPSWMVTVRSLYQKIVREWIIKSLSHAPCTTQGLLQDKLCKANTWQRAQP 1020

Query: 1021 TPDVICLLSEIRIGTSKNEHWTGIQTANIPAVIAAAAAASGADLKLTEAFNLEVLSTGMV 1080
            T DVI LLSEIRIGT KNE WTGIQTANIPAV+AAAAAASGA+ KLTEAFNLEVLSTG+V
Sbjct: 1021 TTDVISLLSEIRIGTMKNECWTGIQTANIPAVMAAAAAASGANFKLTEAFNLEVLSTGIV 1080

Query: 1081 SATVKCNHAGEIAGMRRLYNSIGGFQTG--VAGLGFGQGLQRLITGALPQQPQNEDDSFN 1140
            SATVKCNHAGEIAGMRRLYNSIGGFQ+G    G G G GLQRLI+GA PQQP  EDDSFN
Sbjct: 1081 SATVKCNHAGEIAGMRRLYNSIGGFQSGTTTGGFGIGIGLQRLISGAFPQQPLAEDDSFN 1140

Query: 1141 GILIMKFVQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLASESKTNIEGFAQLIR 1200
            G+L+ KFV+ LQQFV+ +EKG  +DKL FRETCSQATALLLSNL S+SK+NIEGF+QL+R
Sbjct: 1141 GMLLAKFVRMLQQFVNISEKGGDVDKLHFRETCSQATALLLSNLGSDSKSNIEGFSQLLR 1200

Query: 1201 LLCWCPAYISTPDAIETGVFVWTWLVSAAPELGSFVLAELVDAWLWTVDTKRGLFASDVK 1260
            LLCWCPAYISTPDA+ETGVF+WTWLVSAAPELGS VLAELVDAWLWT+DTKRGLFASDVK
Sbjct: 1201 LLCWCPAYISTPDAMETGVFIWTWLVSAAPELGSLVLAELVDAWLWTIDTKRGLFASDVK 1260

Query: 1261 YSGPAAMLRPHLSPGEPEMQPEIDPVEQIIAHKIWLGFFIDRFEVVRHNSVEQLLLFGRL 1320
            YSGPAA LRPHLSPGEPE QPEIDPVEQI+AH+IWLGFFIDRFEVVRHNSVEQLLL GR+
Sbjct: 1261 YSGPAAKLRPHLSPGEPEGQPEIDPVEQIVAHRIWLGFFIDRFEVVRHNSVEQLLLLGRM 1320

Query: 1321 LQGSTKPPWNFSRHPAATGSFFTLMLLGLKFCSCQAQGNLQNFKTGLELLEDRIYRASLG 1380
            LQG+TK PWNFSRHPAA G+FFT+MLLGLKFCSCQ+QGNL NFKTGL+LLEDRIYRASLG
Sbjct: 1321 LQGTTKLPWNFSRHPAAAGTFFTVMLLGLKFCSCQSQGNLHNFKTGLQLLEDRIYRASLG 1380

Query: 1381 WFAHEPEWYDVKHVNFAQSEAQSVSIFLHYLSSERGNSLHSDAKMRGRENGISLIDLNDH 1440
            WFA+E EWYD+ ++NF+QSEAQSVS+F+HYLS+ER +   SD+K+RGRENG +L+D N  
Sbjct: 1381 WFAYEAEWYDINNINFSQSEAQSVSVFVHYLSNERVDGGQSDSKVRGRENGSTLVDANHQ 1440

Query: 1441 YHPVWGHLENYAVGREKRRQLLLMLCQHEADRLEVWAQP-NIKESTPSRPKLTAEKWIEH 1500
            YHPVWG +ENYAVGREKR+QLLLMLCQHEADRLEVWAQP N K+ST SR K+++EKWIE+
Sbjct: 1441 YHPVWGQMENYAVGREKRKQLLLMLCQHEADRLEVWAQPTNSKDSTSSRSKISSEKWIEY 1500

Query: 1501 ARTAFSVDPRIAFSMVSRFPTNAFLRVEMNQLVQLHILDIRSIPEALPYFVTPKAVDENS 1560
            ARTAFSVDPRIA S+ SRFPTN FL+ E+  LVQ +ILDIR IPEALPYFVTPKAVDENS
Sbjct: 1501 ARTAFSVDPRIALSLASRFPTNMFLKAEVTHLVQSNILDIRGIPEALPYFVTPKAVDENS 1560

Query: 1561 ELLRQLPHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQALRY 1620
             LL+QLPHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQ+LRY
Sbjct: 1561 ALLQQLPHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQSLRY 1620

Query: 1621 DEGRLVEGYLLRAAKRSDIFAHILIWHLQGETSLPDSGKDVNSGKNGSFLALLPVVRQRI 1680
            D+ RLVEGYLLRAA+RSD+FAHILIWHLQGET +P+SGK+  SGKN SF  LLP+VR RI
Sbjct: 1621 DDARLVEGYLLRAAQRSDLFAHILIWHLQGETCVPESGKEAISGKNTSFYELLPLVRDRI 1680

Query: 1681 IDGFTPKALDLFKREFDFFDKVTSISGVLFPLPKDERRAGIRSELEKIEMEGEDLYLPTA 1740
            IDGF+PKAL+LFKREFDFFDKVTSISGVLFPLPKDERRAGIR ELEKIE++GEDLYLPTA
Sbjct: 1681 IDGFSPKALELFKREFDFFDKVTSISGVLFPLPKDERRAGIRRELEKIEVDGEDLYLPTA 1740

Query: 1741 TNKLVCGIQVDSGIPLQSAAKVPIMVTFNVVDRDGDPNNIKPQACIFKVGDDCRQDVLAL 1800
             +KLV GIQVDSGIPLQSAAKVPIM+TFNVVDR GD N++KPQACIFKVGDDCRQDVLAL
Sbjct: 1741 PSKLVRGIQVDSGIPLQSAAKVPIMITFNVVDRYGDRNDVKPQACIFKVGDDCRQDVLAL 1800

Query: 1801 QVISLLRDIFQAVGLNLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLYEIFQQ 1860
            QVI+LL DIF+AVG+NLYL+PYGVLPTGP RGIIEVVPNTRSRSQMGETTDGGLYEIFQQ
Sbjct: 1801 QVIALLSDIFKAVGINLYLYPYGVLPTGPERGIIEVVPNTRSRSQMGETTDGGLYEIFQQ 1860

Query: 1861 DYGPVGSPNFEAARENFIVSSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFILET 1920
            D+GPVG+P+FEAARENFI+SSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFILET
Sbjct: 1861 DHGPVGTPSFEAARENFIISSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFILET 1920

Query: 1921 SPGGNMRFESAHFKLSHEMTQLLDPSGVMKSETWNLFVRLCVKGYLAARRHMHGIINTVL 1980
            SPGGNMRFESAHFKLSHEMTQLLDPSGVMKS+TW+ FV LCVKGYLAARR+M GIINTVL
Sbjct: 1921 SPGGNMRFESAHFKLSHEMTQLLDPSGVMKSDTWHQFVSLCVKGYLAARRYMDGIINTVL 1980

Query: 1981 LMLDSGLPCFSRGDPIGNLRKRFHPEMNDREAANFMIRVCTDAYNKWTTAGYDLIQYLQQ 2035
            LMLDSGLPCFSRGDPIGNLRKRFHPEM++REAANFMI VCTDAYNKWTTAGYDLIQYLQQ
Sbjct: 1981 LMLDSGLPCFSRGDPIGNLRKRFHPEMSEREAANFMIHVCTDAYNKWTTAGYDLIQYLQQ 2040

BLAST of Chy1G008030 vs. NCBI nr
Match: XP_011659072.1 (phosphatidylinositol 4-kinase alpha 1 [Cucumis sativus] >KGN44615.2 hypothetical protein Csa_016276 [Cucumis sativus])

HSP 1 Score: 3949 bits (10242), Expect = 0.0
Identity = 2005/2034 (98.57%), Postives = 2016/2034 (99.12%), Query Frame = 0

Query: 1    MDALIELCDLIAQNPALFSEKLSWICSRCPPSEAILAGSPAISRSQLNAVLAVARLLSKC 60
            MDALIELCDLIAQ+PALFSEKLSWICSRCPPSEAILAGSPAISRSQLNAVLAVARLLSKC
Sbjct: 1    MDALIELCDLIAQSPALFSEKLSWICSRCPPSEAILAGSPAISRSQLNAVLAVARLLSKC 60

Query: 61   PDSVGLRPKSVVLEFLRSIPLSFSLSFWPQSYGNDAIASFFNEFLNYTSKACALSTDFAT 120
            PDSVGLRPKSVVLEFLRSIPLSFSLSFWPQSYGNDAIASFFNEFLNYTSKAC LSTDFAT
Sbjct: 61   PDSVGLRPKSVVLEFLRSIPLSFSLSFWPQSYGNDAIASFFNEFLNYTSKACELSTDFAT 120

Query: 121  EVSGFSSEVVLSAINDCSEGSAISRAFLVALSKSFLPIIPSDADKLVSCILDRFLISEAA 180
            EVSGFSSEVVLSAINDCSEGSAISRAFLVALSKSFLPIIPSDADKLVSCILDRFLISEAA
Sbjct: 121  EVSGFSSEVVLSAINDCSEGSAISRAFLVALSKSFLPIIPSDADKLVSCILDRFLISEAA 180

Query: 181  PGTPREHNQANSEPSSSQSSPLSVSHQPSNGGLSPGNENGQVSGSLSSGASRITDDATSA 240
            PGTPREHNQANSEPSSSQSSPLSVSHQPSNGGLSPGNENGQVSGSLSSGASRITDDATSA
Sbjct: 181  PGTPREHNQANSEPSSSQSSPLSVSHQPSNGGLSPGNENGQVSGSLSSGASRITDDATSA 240

Query: 241  SSRGSGMMNGNSILWKSGLEQLGMNLGFSEGGGVAFVRQQVALFVDESIENLEKQEIAFK 300
            SSRGSGMMNGNSILW+SGLEQ      FSEGGGVAFVRQQVALF DESIENLEKQEIAFK
Sbjct: 241  SSRGSGMMNGNSILWRSGLEQ------FSEGGGVAFVRQQVALFEDESIENLEKQEIAFK 300

Query: 301  LMTHILDNSSFDARLWEQMRALAKKQLQTLPTFLKIKKRDWSEQGSVLKARINTKLLVYQ 360
            LMTHILDNSSFD RLWEQMRALAKKQLQTLPTFLKIKKRDWSEQGSVLKARINTKLLVYQ
Sbjct: 301  LMTHILDNSSFDGRLWEQMRALAKKQLQTLPTFLKIKKRDWSEQGSVLKARINTKLLVYQ 360

Query: 361  AAARLKMKTVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFSSLLTGL 420
            AAARLKMKTVASLDFDGKPAKKLIFETFALLMDAA+ACLLSVWRKLRICEELF SLLTGL
Sbjct: 361  AAARLKMKTVASLDFDGKPAKKLIFETFALLMDAADACLLSVWRKLRICEELFGSLLTGL 420

Query: 421  AQIAVARGGQPLRVLLIRLKPLVLTVCVQADTWGTNQGAMFESVLTTCCEIIESCWTKDR 480
            AQIAVARGGQPLRVLLIRLKPLVLTVCVQADTWGTNQGAMFESVLTTCCEIIESCWTKDR
Sbjct: 421  AQIAVARGGQPLRVLLIRLKPLVLTVCVQADTWGTNQGAMFESVLTTCCEIIESCWTKDR 480

Query: 481  APVDTFIMGLATSIRDRNDSEEQDDKEKQGVPMQLNVIRLLAKMTVAVNKSEIVDMILPL 540
            APVDTFIMGLATSIRDRNDSEEQDDKEKQGVPMQLNVIRLLAKMTVAVNKSEIVDMILPL
Sbjct: 481  APVDTFIMGLATSIRDRNDSEEQDDKEKQGVPMQLNVIRLLAKMTVAVNKSEIVDMILPL 540

Query: 541  FIESLEEGDASTPGLLRLQLLDAVSRMATLGFEKSYRETIVLMTRSYLSKLSSIGSSESR 600
            FIESLEEGDASTPGLLRLQLLDAVSRMATLGFEKSYRETIVLMTRSYLSKLSSIGSSESR
Sbjct: 541  FIESLEEGDASTPGLLRLQLLDAVSRMATLGFEKSYRETIVLMTRSYLSKLSSIGSSESR 600

Query: 601  TVAPEATTERVEILPAGFLHIASGLKSAKLRLEYRHRLLSLCSDVGLAAESKSGRSGANF 660
            TVAPEATTERVEILPAGFLHIA+GLKSAKLRLEYRHRLLSLCSDVGLAAESKSGRSGA+F
Sbjct: 601  TVAPEATTERVEILPAGFLHIANGLKSAKLRLEYRHRLLSLCSDVGLAAESKSGRSGADF 660

Query: 661  LGPLLPAVAEICSDFDPTMDIEPSLLKLFRNLWFYIALFGLAPPIQNSHLQTKSVSTMLN 720
            LGPLLPAVAEICSDFDPTM+IEPSLLKLFRNLWFYIALFGLAPPIQ SHLQTKSVSTMLN
Sbjct: 661  LGPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQKSHLQTKSVSTMLN 720

Query: 721  SVGSTAIALQAVSGPYLWNTQWSSAVQLIARGTPPLVVSSVKWLEDELELNALHNPGSRR 780
            SVGSTAIALQAVSGPYLWNTQWSSAVQLIARGTPPLVVSSVKWLEDELELNALHNPGSRR
Sbjct: 721  SVGSTAIALQAVSGPYLWNTQWSSAVQLIARGTPPLVVSSVKWLEDELELNALHNPGSRR 780

Query: 781  GSGNEKAALAQRAALSSALGGRVDVAAMSTISGVKATYLLAVSFLEIIRFSSNGGILNGG 840
            GSGNEKAALAQRAALS+ALGGRVDVAAMSTISGVKATYLLAVSFLEIIRFSSNGGILNGG
Sbjct: 781  GSGNEKAALAQRAALSAALGGRVDVAAMSTISGVKATYLLAVSFLEIIRFSSNGGILNGG 840

Query: 841  SNVNASRSAFCCVFEYLKTPNLLPAVSQCLTAIVHRAFETAVLWLEDRISDTGNEAEVRD 900
            SNVNASRSAFCCVFEYLKTPNLLPAVSQCLTAIVHRAFETAVLWLEDRISDTGNEAEVRD
Sbjct: 841  SNVNASRSAFCCVFEYLKTPNLLPAVSQCLTAIVHRAFETAVLWLEDRISDTGNEAEVRD 900

Query: 901  STLFAHTCYLIKSMSQRDEHVRDIAVNLLTQLRDKFPQVMWNSSCLDSLLFSMHNDAPST 960
            STLFAHTCYLIKSMSQRDEHVRDIAVNLLTQLRDKFPQVMWNSSCLDSLLFSMHNDAPST
Sbjct: 901  STLFAHTCYLIKSMSQRDEHVRDIAVNLLTQLRDKFPQVMWNSSCLDSLLFSMHNDAPST 960

Query: 961  VVTDPAWVVTVRSLYQRVVREWIVKSLSYAPCTCQGLLQEKLCKANTWQRAQHTPDVICL 1020
            VVTDPAWVVTVRSLYQRVVREWIVKSLSYAPCTCQGLLQEKLCKANTWQRAQHTPDVI L
Sbjct: 961  VVTDPAWVVTVRSLYQRVVREWIVKSLSYAPCTCQGLLQEKLCKANTWQRAQHTPDVISL 1020

Query: 1021 LSEIRIGTSKNEHWTGIQTANIPAVIAAAAAASGADLKLTEAFNLEVLSTGMVSATVKCN 1080
            LSEIRIGTSKNEHWTGIQTANIPAVI AAAAASGADLKLTEAFNLEVLSTGMVSATVKCN
Sbjct: 1021 LSEIRIGTSKNEHWTGIQTANIPAVITAAAAASGADLKLTEAFNLEVLSTGMVSATVKCN 1080

Query: 1081 HAGEIAGMRRLYNSIGGFQTGVAGLGFGQGLQRLITGALPQQPQNEDDSFNGILIMKFVQ 1140
            HAGEIAGMRRLYNSIGGFQTGVAGLGFGQGLQRLITGALPQQPQNEDDSFNGILIMKFVQ
Sbjct: 1081 HAGEIAGMRRLYNSIGGFQTGVAGLGFGQGLQRLITGALPQQPQNEDDSFNGILIMKFVQ 1140

Query: 1141 SLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLASESKTNIEGFAQLIRLLCWCPAYI 1200
            SLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLASESKTNIEGFAQLIRLLCWCPAYI
Sbjct: 1141 SLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLASESKTNIEGFAQLIRLLCWCPAYI 1200

Query: 1201 STPDAIETGVFVWTWLVSAAPELGSFVLAELVDAWLWTVDTKRGLFASDVKYSGPAAMLR 1260
            STPDAIETGVF+WTWLVSAAPELGSFVLAELVDAWLWT+DTKRGLFASDVKYSGPAAMLR
Sbjct: 1201 STPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRGLFASDVKYSGPAAMLR 1260

Query: 1261 PHLSPGEPEMQPEIDPVEQIIAHKIWLGFFIDRFEVVRHNSVEQLLLFGRLLQGSTKPPW 1320
            PHLSPGEPEMQPEIDPVEQIIAH+IWLGFFIDRFEVVRHNSVEQLLLFGRLLQGSTKPPW
Sbjct: 1261 PHLSPGEPEMQPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQLLLFGRLLQGSTKPPW 1320

Query: 1321 NFSRHPAATGSFFTLMLLGLKFCSCQAQGNLQNFKTGLELLEDRIYRASLGWFAHEPEWY 1380
            NFSRHPAATGSFFTLMLLGLKFCSCQAQGNLQNFKTGLELLEDRIYRASLGWFAHEPEWY
Sbjct: 1321 NFSRHPAATGSFFTLMLLGLKFCSCQAQGNLQNFKTGLELLEDRIYRASLGWFAHEPEWY 1380

Query: 1381 DVKHVNFAQSEAQSVSIFLHYLSSERGNSLHSDAKMRGRENGISLIDLNDHYHPVWGHLE 1440
            DVKHVNFAQSEAQSVSIFLHYLSSERGNSLHSDAKMRGRENGISLIDLNDHYHPVWGHLE
Sbjct: 1381 DVKHVNFAQSEAQSVSIFLHYLSSERGNSLHSDAKMRGRENGISLIDLNDHYHPVWGHLE 1440

Query: 1441 NYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTPSRPKLTAEKWIEHARTAFSVDPR 1500
            NYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTPSRPKLTAEKWIEHARTAFSVDPR
Sbjct: 1441 NYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTPSRPKLTAEKWIEHARTAFSVDPR 1500

Query: 1501 IAFSMVSRFPTNAFLRVEMNQLVQLHILDIRSIPEALPYFVTPKAVDENSELLRQLPHWA 1560
            IAFSMVSRFPTNAFLRVEMNQLVQLHILDIRSIPEALPYFVTPKAVDENSELLRQLPHWA
Sbjct: 1501 IAFSMVSRFPTNAFLRVEMNQLVQLHILDIRSIPEALPYFVTPKAVDENSELLRQLPHWA 1560

Query: 1561 ACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQALRYDEGRLVEGYL 1620
            ACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQALRYDEGRLVEGYL
Sbjct: 1561 ACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQALRYDEGRLVEGYL 1620

Query: 1621 LRAAKRSDIFAHILIWHLQGETSLPDSGKDVNSGKNGSFLALLPVVRQRIIDGFTPKALD 1680
            LRAAKRSDIFAHILIWHLQGETSLPDSGKDVNSGKNGSFLALLPVVRQ IIDGFTPKALD
Sbjct: 1621 LRAAKRSDIFAHILIWHLQGETSLPDSGKDVNSGKNGSFLALLPVVRQHIIDGFTPKALD 1680

Query: 1681 LFKREFDFFDKVTSISGVLFPLPKDERRAGIRSELEKIEMEGEDLYLPTATNKLVCGIQV 1740
            LFKREFDFFDKVTSISGVLFPLPKDERRAGIRSELEKIEMEGEDLYLPTATNKLV GIQV
Sbjct: 1681 LFKREFDFFDKVTSISGVLFPLPKDERRAGIRSELEKIEMEGEDLYLPTATNKLVRGIQV 1740

Query: 1741 DSGIPLQSAAKVPIMVTFNVVDRDGDPNNIKPQACIFKVGDDCRQDVLALQVISLLRDIF 1800
            DSGIPLQSAAKVPIMVTFNVVDRDGDPNNIKPQACIFKVGDDCRQDVLALQVISLLRDIF
Sbjct: 1741 DSGIPLQSAAKVPIMVTFNVVDRDGDPNNIKPQACIFKVGDDCRQDVLALQVISLLRDIF 1800

Query: 1801 QAVGLNLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGPVGSPNF 1860
            QAVGLNLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGPVGSP+F
Sbjct: 1801 QAVGLNLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGPVGSPSF 1860

Query: 1861 EAARENFIVSSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFILETSPGGNMRFES 1920
            EAARENFIVSSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFILETSPGGNMRFES
Sbjct: 1861 EAARENFIVSSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFILETSPGGNMRFES 1920

Query: 1921 AHFKLSHEMTQLLDPSGVMKSETWNLFVRLCVKGYLAARRHMHGIINTVLLMLDSGLPCF 1980
            AHFKLSHEMTQLLDPSGVMKSETWNLFV LCVKGYL ARRHM GIINTVLLMLDSGLPCF
Sbjct: 1921 AHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLTARRHMDGIINTVLLMLDSGLPCF 1980

Query: 1981 SRGDPIGNLRKRFHPEMNDREAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK 2034
            SRGDPIGNLRKRFHPEMNDREAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK
Sbjct: 1981 SRGDPIGNLRKRFHPEMNDREAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK 2028

BLAST of Chy1G008030 vs. NCBI nr
Match: XP_008447043.1 (PREDICTED: phosphatidylinositol 4-kinase alpha 1-like [Cucumis melo])

HSP 1 Score: 3927 bits (10183), Expect = 0.0
Identity = 1993/2037 (97.84%), Postives = 2009/2037 (98.63%), Query Frame = 0

Query: 1    MDALIELCDLIAQNPALFSEKLSWICSRCPPSEAILAGSPAISRSQLNAVLAVARLLSKC 60
            MDALIELCDLIAQNPALFSEKLSWICSRCPPSEAILAGSP ISRSQLNAVLAVARLLSKC
Sbjct: 1    MDALIELCDLIAQNPALFSEKLSWICSRCPPSEAILAGSPTISRSQLNAVLAVARLLSKC 60

Query: 61   PDSVGLRPKSVVLEFLRSIPLSFSLSFWPQSYGNDAIASFFNEFLNYTSKACALSTDFAT 120
            PDSVGLRPKSVVLEFLRSIPLSFSLSFWPQSYG+DAIASFFNEFLNYTSKAC LSTDFAT
Sbjct: 61   PDSVGLRPKSVVLEFLRSIPLSFSLSFWPQSYGHDAIASFFNEFLNYTSKACELSTDFAT 120

Query: 121  EVSGFSSEVVLSAINDCSEGSAISRAFLVALSKSFLPIIPSDADKLVSCILDRFLISEAA 180
            EVSGFSSEVVLSAINDCSEGSAISRAFLVAL+KSFLPIIPSDADKLVSCILDRFLISEA 
Sbjct: 121  EVSGFSSEVVLSAINDCSEGSAISRAFLVALTKSFLPIIPSDADKLVSCILDRFLISEAI 180

Query: 181  PGTPREHNQANSEPSSSQSSPLSVSHQPSNGGLSPGNENGQVSGSLSSGASRITDDATSA 240
            P TPREHNQANSEPSSSQSSPLSVSHQPSNGGLSPGNENGQVSGSLSSGASRITDDATSA
Sbjct: 181  PETPREHNQANSEPSSSQSSPLSVSHQPSNGGLSPGNENGQVSGSLSSGASRITDDATSA 240

Query: 241  SSRGSGMMNGNSILWKSGLEQLGMNLGFSEGGGVAFVRQQVALFVDESIENLEKQEIAFK 300
            SSRGSGMMNGNSILWKSGLEQLGMNLGFSEGGGVAFVRQQVALF DESIENLEKQEIAFK
Sbjct: 241  SSRGSGMMNGNSILWKSGLEQLGMNLGFSEGGGVAFVRQQVALFEDESIENLEKQEIAFK 300

Query: 301  LMTHILDNSSFDARLWEQMRALAKKQLQTLPTFLKIKKRDWSEQGSVLKARINTKLLVYQ 360
            LMTHILDNSSFDARLWEQM+ LAKKQLQTLPTFLKIKKRDWSEQGSVLKARINTKLLVYQ
Sbjct: 301  LMTHILDNSSFDARLWEQMKTLAKKQLQTLPTFLKIKKRDWSEQGSVLKARINTKLLVYQ 360

Query: 361  AAARLKMKTVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFSSLLTGL 420
            AAARLKMKTVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELF SLLTGL
Sbjct: 361  AAARLKMKTVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFGSLLTGL 420

Query: 421  AQIAVARGGQPLRVLLIRLKPLVLTVCVQADTWGTNQGAMFESVLTTCCEIIESCWTKDR 480
            AQIAVARGGQPLRVLLIRLKPLVLTVCVQADTWGTNQGAMFESVLTTCCEIIESCWTKDR
Sbjct: 421  AQIAVARGGQPLRVLLIRLKPLVLTVCVQADTWGTNQGAMFESVLTTCCEIIESCWTKDR 480

Query: 481  APVDTFIMGLATSIRDRNDSEEQDDKEKQGVPMQLNVIRLLAKMTVAVNKSEIVDMILPL 540
            APVDTFIMGLATSIRDRNDSEEQDDKEKQGVPMQLNVIRLLAKMTVAVNKSEIVDMILPL
Sbjct: 481  APVDTFIMGLATSIRDRNDSEEQDDKEKQGVPMQLNVIRLLAKMTVAVNKSEIVDMILPL 540

Query: 541  FIESLEEGDASTPGLLRLQLLDAVSRMATLGFEKSYRETIVLMTRSYLSKLSSIGSSESR 600
            FIESLEEGDASTPGLLRLQLLDAVSRMATLGFEKSYRETIVLMTRSYLSKLSSIGSSESR
Sbjct: 541  FIESLEEGDASTPGLLRLQLLDAVSRMATLGFEKSYRETIVLMTRSYLSKLSSIGSSESR 600

Query: 601  TVAPEATTERVEILPAGFLHIASGLKSAKLRLEYRHRLLSLCSDVGLAAESKSGRSGANF 660
            TVAPEATTERVEILPAGFLHIA+GLKSAKLRLEYRHRLLSLCSDVGLAAESKSGRSGA+F
Sbjct: 601  TVAPEATTERVEILPAGFLHIANGLKSAKLRLEYRHRLLSLCSDVGLAAESKSGRSGADF 660

Query: 661  LGPLLPAVAEICSDFDPTMDIEPSLLKLFRNLWFYIALFGLAPPIQNSHLQTKSVSTMLN 720
            LGPLLPAVAEICSDFDPTM+IEPSLLKLFRNLWFYIALFGLAPPIQ S LQTKSVSTMLN
Sbjct: 661  LGPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQKSQLQTKSVSTMLN 720

Query: 721  SVGSTAIALQAVSGPYLWNTQWSSAVQLIARGTPPLVVSSVKWLEDELELNALHNPGSRR 780
            SVGSTAIALQAVSGPYLWNTQWSSAVQ IARGTPPLVVSSVKWLEDELELNALHNPGSRR
Sbjct: 721  SVGSTAIALQAVSGPYLWNTQWSSAVQFIARGTPPLVVSSVKWLEDELELNALHNPGSRR 780

Query: 781  GSGNEKAALAQRAALSSALGGRVDVAAMSTISGVKATYLLAVSFLEIIRFSSNGGILNGG 840
            GSGNEKAALAQRAALS+ALGGRVDVAAMSTISGVKATYLLAVSFLEIIRFSSNGGILNGG
Sbjct: 781  GSGNEKAALAQRAALSAALGGRVDVAAMSTISGVKATYLLAVSFLEIIRFSSNGGILNGG 840

Query: 841  SNVNASRSAFCCVFEYLKTPNLLPAVSQCLTAIVHRAFETAVLWLEDRISDTGNEAEVRD 900
            SNVNASRSAFCCVFEYLKTPNLLPAVSQCLTAIVHRAFETAV WLEDRISDTGNEAE RD
Sbjct: 841  SNVNASRSAFCCVFEYLKTPNLLPAVSQCLTAIVHRAFETAVSWLEDRISDTGNEAEARD 900

Query: 901  STLFAHTCYLIKSMSQRDEHVRDIAVNLLTQLRDKFPQVMWNSSCLDSLLFSMHNDAPST 960
            STLFAHTCYLIKSMSQRDEHVRDIAVNLLTQLR+KFPQVMWNSSCLDSLLFSMHNDAPST
Sbjct: 901  STLFAHTCYLIKSMSQRDEHVRDIAVNLLTQLREKFPQVMWNSSCLDSLLFSMHNDAPST 960

Query: 961  VVTDPAWVVTVRSLYQRVVREWIVKSLSYAPCTCQGLLQEKLCKANTWQRAQHTPDVICL 1020
            VV+DPAWVVTVRSLYQRVVREWIVKSLSYAPCTCQGLLQEKLCKANTWQRAQHTPDVI L
Sbjct: 961  VVSDPAWVVTVRSLYQRVVREWIVKSLSYAPCTCQGLLQEKLCKANTWQRAQHTPDVISL 1020

Query: 1021 LSEIRIGTSKNEHWTGIQTANIPAVIAAAAAASGADLKLTEAFNLEVLSTGMVSATVKCN 1080
            LSEIRIGTSKNEHWTG QTANIPAVI AAAAASGADLKLTEAFNLEVLSTGMVSATVKCN
Sbjct: 1021 LSEIRIGTSKNEHWTGTQTANIPAVITAAAAASGADLKLTEAFNLEVLSTGMVSATVKCN 1080

Query: 1081 HAGEIAGMRRLYNSIGGFQTGVAG---LGFGQGLQRLITGALPQQPQNEDDSFNGILIMK 1140
            HAGEIAGMRRLYNSIGGFQTGVAG   LGFGQGLQRLITGALPQQPQNEDDSFNGILIMK
Sbjct: 1081 HAGEIAGMRRLYNSIGGFQTGVAGSTGLGFGQGLQRLITGALPQQPQNEDDSFNGILIMK 1140

Query: 1141 FVQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLASESKTNIEGFAQLIRLLCWCP 1200
            FVQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNL SESKTNIEGFAQLIRLLCWCP
Sbjct: 1141 FVQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLDSESKTNIEGFAQLIRLLCWCP 1200

Query: 1201 AYISTPDAIETGVFVWTWLVSAAPELGSFVLAELVDAWLWTVDTKRGLFASDVKYSGPAA 1260
            AYISTPDAIETGVF+WTWLVSAAPELGSFVLAELVDAWLWT+DTKRGLFASD+KYSGPAA
Sbjct: 1201 AYISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRGLFASDIKYSGPAA 1260

Query: 1261 MLRPHLSPGEPEMQPEIDPVEQIIAHKIWLGFFIDRFEVVRHNSVEQLLLFGRLLQGSTK 1320
            MLRPHLSPGEPEMQPEIDPVEQIIAH+IWLGFFIDRFEVVRHNSVEQLLLFGRLLQGSTK
Sbjct: 1261 MLRPHLSPGEPEMQPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQLLLFGRLLQGSTK 1320

Query: 1321 PPWNFSRHPAATGSFFTLMLLGLKFCSCQAQGNLQNFKTGLELLEDRIYRASLGWFAHEP 1380
            PPWNFSRHPAATGSFFTLMLLGLKFCSCQAQGNLQNFKTGLELLEDRIYRASLGWFAHEP
Sbjct: 1321 PPWNFSRHPAATGSFFTLMLLGLKFCSCQAQGNLQNFKTGLELLEDRIYRASLGWFAHEP 1380

Query: 1381 EWYDVKHVNFAQSEAQSVSIFLHYLSSERGNSLHSDAKMRGRENGISLIDLNDHYHPVWG 1440
            EWYDVKHVNFAQSEAQSVSIFLHYLSSERGNSL SDAK+RGRENGISLID NDHYHPVWG
Sbjct: 1381 EWYDVKHVNFAQSEAQSVSIFLHYLSSERGNSLQSDAKIRGRENGISLIDSNDHYHPVWG 1440

Query: 1441 HLENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTPSRPKLTAEKWIEHARTAFSV 1500
            HLENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTP RPKLTAEKWIEHARTAFSV
Sbjct: 1441 HLENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTPYRPKLTAEKWIEHARTAFSV 1500

Query: 1501 DPRIAFSMVSRFPTNAFLRVEMNQLVQLHILDIRSIPEALPYFVTPKAVDENSELLRQLP 1560
            DPRIAFSMVSRFPTNAFLRVEMNQLVQLHILDIRSIPEALPYFVTPKAVDENSELLRQLP
Sbjct: 1501 DPRIAFSMVSRFPTNAFLRVEMNQLVQLHILDIRSIPEALPYFVTPKAVDENSELLRQLP 1560

Query: 1561 HWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQALRYDEGRLVE 1620
            HWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQALRYDEGRLVE
Sbjct: 1561 HWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQALRYDEGRLVE 1620

Query: 1621 GYLLRAAKRSDIFAHILIWHLQGETSLPDSGKDVNSGKNGSFLALLPVVRQRIIDGFTPK 1680
            GYLLRAAKRSDIFAHILIWHLQGETS PDSGKDVNSGKNGSFLALLPVVRQ IIDGFTPK
Sbjct: 1621 GYLLRAAKRSDIFAHILIWHLQGETSSPDSGKDVNSGKNGSFLALLPVVRQHIIDGFTPK 1680

Query: 1681 ALDLFKREFDFFDKVTSISGVLFPLPKDERRAGIRSELEKIEMEGEDLYLPTATNKLVCG 1740
            ALDLF+REFDFFDKVTSISGVLFPLPKDERRAGIR ELEKIEM+GEDLYLPTATNKLV G
Sbjct: 1681 ALDLFRREFDFFDKVTSISGVLFPLPKDERRAGIRRELEKIEMDGEDLYLPTATNKLVRG 1740

Query: 1741 IQVDSGIPLQSAAKVPIMVTFNVVDRDGDPNNIKPQACIFKVGDDCRQDVLALQVISLLR 1800
            IQVDSGIPLQSAAKVPIMVTFNVVDRDGDPNNIKPQACIFKVGDDCRQDVLALQVISLLR
Sbjct: 1741 IQVDSGIPLQSAAKVPIMVTFNVVDRDGDPNNIKPQACIFKVGDDCRQDVLALQVISLLR 1800

Query: 1801 DIFQAVGLNLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGPVGS 1860
            DIFQAVGLNLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGPVGS
Sbjct: 1801 DIFQAVGLNLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGPVGS 1860

Query: 1861 PNFEAARENFIVSSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFILETSPGGNMR 1920
            PNFEAARENFIVSSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFILETSPGGNMR
Sbjct: 1861 PNFEAARENFIVSSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFILETSPGGNMR 1920

Query: 1921 FESAHFKLSHEMTQLLDPSGVMKSETWNLFVRLCVKGYLAARRHMHGIINTVLLMLDSGL 1980
            FESAHFKLSHEMTQLLDPSGVMKSETWNLFV LCVKGYLAARRHM GIINTVLLMLDSGL
Sbjct: 1921 FESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMDGIINTVLLMLDSGL 1980

Query: 1981 PCFSRGDPIGNLRKRFHPEMNDREAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK 2034
            PCFSRGDPIGNLRKRFHPEMNDREAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK
Sbjct: 1981 PCFSRGDPIGNLRKRFHPEMNDREAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK 2037

BLAST of Chy1G008030 vs. NCBI nr
Match: XP_038887703.1 (phosphatidylinositol 4-kinase alpha 1-like [Benincasa hispida])

HSP 1 Score: 3888 bits (10083), Expect = 0.0
Identity = 1973/2037 (96.86%), Postives = 2003/2037 (98.33%), Query Frame = 0

Query: 1    MDALIELCDLIAQNPALFSEKLSWICSRCPPSEAILAGSPAISRSQLNAVLAVARLLSKC 60
            MDALIELCDLIAQNPALFSEKLSWICSRCPPSEAILAGSPAISRSQLNAVLAVARLLSKC
Sbjct: 1    MDALIELCDLIAQNPALFSEKLSWICSRCPPSEAILAGSPAISRSQLNAVLAVARLLSKC 60

Query: 61   PDSVGLRPKSVVLEFLRSIPLSFSLSFWPQSYGNDAIASFFNEFLNYTSKACALSTDFAT 120
            PDSVGLRPKSVVLEFLRSIPLSFSLSFWPQSYGNDAIASFFNEFLNYTSKAC LSTDFA 
Sbjct: 61   PDSVGLRPKSVVLEFLRSIPLSFSLSFWPQSYGNDAIASFFNEFLNYTSKACELSTDFAM 120

Query: 121  EVSGFSSEVVLSAINDCSEGSAISRAFLVALSKSFLPIIPSDADKLVSCILDRFLISEAA 180
            E+SGFSSEVVLSAINDC EGSAISRAFLVALSKSFLPIIPSDADKLVSCILDRFLISEAA
Sbjct: 121  EISGFSSEVVLSAINDCCEGSAISRAFLVALSKSFLPIIPSDADKLVSCILDRFLISEAA 180

Query: 181  PGTPREHNQANSEPSSSQSSPLSVSHQPSNGGLSPGNENGQVSGSLSSGASRITDDATSA 240
            PGTPREHNQANSE SSSQSSPLSVSHQPSNGGLSPGNENGQVSGSLSSGASRITDDATSA
Sbjct: 181  PGTPREHNQANSEASSSQSSPLSVSHQPSNGGLSPGNENGQVSGSLSSGASRITDDATSA 240

Query: 241  SSRGSGMMNGNSILWKSGLEQLGMNLGFSEGGGVAFVRQQVALFVDESIENLEKQEIAFK 300
            SSRGSGMMNGNSILWKSGLEQLGMN GFSEGGGVAFVRQQVA F DESIENLEKQEIAFK
Sbjct: 241  SSRGSGMMNGNSILWKSGLEQLGMNQGFSEGGGVAFVRQQVASFEDESIENLEKQEIAFK 300

Query: 301  LMTHILDNSSFDARLWEQMRALAKKQLQTLPTFLKIKKRDWSEQGSVLKARINTKLLVYQ 360
            LMTHILDNSSFDARLWEQMRALAKKQLQTLPTFLKIKKRDWSEQGSVLKARINTKLLVYQ
Sbjct: 301  LMTHILDNSSFDARLWEQMRALAKKQLQTLPTFLKIKKRDWSEQGSVLKARINTKLLVYQ 360

Query: 361  AAARLKMKTVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFSSLLTGL 420
            AAARLKMKTV SLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELF SLLTGL
Sbjct: 361  AAARLKMKTVVSLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFGSLLTGL 420

Query: 421  AQIAVARGGQPLRVLLIRLKPLVLTVCVQADTWGTNQGAMFESVLTTCCEIIESCWTKDR 480
            +QIAV+RGGQPLRVLLIRLKPLVLTVC QADTWG NQGAMFESVLTTCCEIIESCWTKDR
Sbjct: 421  SQIAVSRGGQPLRVLLIRLKPLVLTVCAQADTWG-NQGAMFESVLTTCCEIIESCWTKDR 480

Query: 481  APVDTFIMGLATSIRDRNDSEEQDDKEKQGVPMQLNVIRLLAKMTVAVNKSEIVDMILPL 540
            APVDTFIMGLATSIRDRNDSEEQDDKEKQ VPMQLNVIRLLAKMTVAVNKSEIVDMILPL
Sbjct: 481  APVDTFIMGLATSIRDRNDSEEQDDKEKQAVPMQLNVIRLLAKMTVAVNKSEIVDMILPL 540

Query: 541  FIESLEEGDASTPGLLRLQLLDAVSRMATLGFEKSYRETIVLMTRSYLSKLSSIGSSESR 600
            FIESLEEGDASTPGLLRLQLLDAVSRMA+LGFEKSYRETIVLMTRSYLSKLSSIGSSESR
Sbjct: 541  FIESLEEGDASTPGLLRLQLLDAVSRMASLGFEKSYRETIVLMTRSYLSKLSSIGSSESR 600

Query: 601  TVAPEATTERVEILPAGFLHIASGLKSAKLRLEYRHRLLSLCSDVGLAAESKSGRSGANF 660
            TVAPEATTERVEILPAGFL IA+ LKSAKLRLEYRHRLLSLCSDVGLAAESKSGRSGA+F
Sbjct: 601  TVAPEATTERVEILPAGFLLIANSLKSAKLRLEYRHRLLSLCSDVGLAAESKSGRSGADF 660

Query: 661  LGPLLPAVAEICSDFDPTMDIEPSLLKLFRNLWFYIALFGLAPPIQNSHLQTKSVSTMLN 720
            LGPLLPAVAEICSDFDPTM+IEPSLLKLFRNLWFYIALFGLAPPIQ S LQTKSVSTMLN
Sbjct: 661  LGPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQKSQLQTKSVSTMLN 720

Query: 721  SVGSTAIALQAVSGPYLWNTQWSSAVQLIARGTPPLVVSSVKWLEDELELNALHNPGSRR 780
            SVGSTAIALQAVSGPYLWNTQWSSAVQ IARGTPPLVVSSVKWLEDELELNALHNPGSRR
Sbjct: 721  SVGSTAIALQAVSGPYLWNTQWSSAVQFIARGTPPLVVSSVKWLEDELELNALHNPGSRR 780

Query: 781  GSGNEKAALAQRAALSSALGGRVDVAAMSTISGVKATYLLAVSFLEIIRFSSNGGILNGG 840
            GSGNEKAA+AQRAALS+ALGGRVDVAAMSTISGVKATYLLAVSFLEIIRFSSNGGILNGG
Sbjct: 781  GSGNEKAAVAQRAALSAALGGRVDVAAMSTISGVKATYLLAVSFLEIIRFSSNGGILNGG 840

Query: 841  SNVNASRSAFCCVFEYLKTPNLLPAVSQCLTAIVHRAFETAVLWLEDRISDTGNEAEVRD 900
            SNVNASRSAFCCVFEYLKTPNLLPAVSQCLTAIVHRAFETAVLWLEDRIS+TGNEAEVRD
Sbjct: 841  SNVNASRSAFCCVFEYLKTPNLLPAVSQCLTAIVHRAFETAVLWLEDRISETGNEAEVRD 900

Query: 901  STLFAHTCYLIKSMSQRDEHVRDIAVNLLTQLRDKFPQVMWNSSCLDSLLFSMHNDAPST 960
            STLFAHTCYLIKSMSQRDEHVRDIAVNLLTQLRDKFPQVMWNSSCLDSLLFSMHNDAPST
Sbjct: 901  STLFAHTCYLIKSMSQRDEHVRDIAVNLLTQLRDKFPQVMWNSSCLDSLLFSMHNDAPST 960

Query: 961  VVTDPAWVVTVRSLYQRVVREWIVKSLSYAPCTCQGLLQEKLCKANTWQRAQHTPDVICL 1020
            V++DPAWVVTVRSLYQRVVREWIVKSLSYAPCTCQGLLQEKLCKANTWQR QHTPDVI L
Sbjct: 961  VISDPAWVVTVRSLYQRVVREWIVKSLSYAPCTCQGLLQEKLCKANTWQRGQHTPDVISL 1020

Query: 1021 LSEIRIGTSKNEHWTGIQTANIPAVIAAAAAASGADLKLTEAFNLEVLSTGMVSATVKCN 1080
            LSEIRIGTSKN++WTGIQTANIPAVI AAAAASGADLKLTEAFNLEVLSTGMVSATVKCN
Sbjct: 1021 LSEIRIGTSKNDYWTGIQTANIPAVITAAAAASGADLKLTEAFNLEVLSTGMVSATVKCN 1080

Query: 1081 HAGEIAGMRRLYNSIGGFQTGVAG---LGFGQGLQRLITGALPQQPQNEDDSFNGILIMK 1140
            HAGEIAGMRRLYNSIGGFQTGVAG   LGFGQGLQRLITGA+PQQPQNEDDSFNGILIMK
Sbjct: 1081 HAGEIAGMRRLYNSIGGFQTGVAGPTGLGFGQGLQRLITGAVPQQPQNEDDSFNGILIMK 1140

Query: 1141 FVQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLASESKTNIEGFAQLIRLLCWCP 1200
            FVQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLASESKTNIEGFAQLIRLLCWCP
Sbjct: 1141 FVQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLASESKTNIEGFAQLIRLLCWCP 1200

Query: 1201 AYISTPDAIETGVFVWTWLVSAAPELGSFVLAELVDAWLWTVDTKRGLFASDVKYSGPAA 1260
            AYISTPDAIETGVF+WTWLVSAAPELGSFVLAELVDAWLWT+DTKRGLFAS+VKYSGPAA
Sbjct: 1201 AYISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRGLFASEVKYSGPAA 1260

Query: 1261 MLRPHLSPGEPEMQPEIDPVEQIIAHKIWLGFFIDRFEVVRHNSVEQLLLFGRLLQGSTK 1320
             LRPHLSPGEPE+QPEIDPVEQIIAH+IWLGFFIDRFEVVRHNSVEQLLLFGRLLQGSTK
Sbjct: 1261 KLRPHLSPGEPEVQPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQLLLFGRLLQGSTK 1320

Query: 1321 PPWNFSRHPAATGSFFTLMLLGLKFCSCQAQGNLQNFKTGLELLEDRIYRASLGWFAHEP 1380
            PPWNFSRHPAATGSFFTLMLLGLKFCSCQ+QGNLQNFKTGLELLEDR+YRASLGWFAHEP
Sbjct: 1321 PPWNFSRHPAATGSFFTLMLLGLKFCSCQSQGNLQNFKTGLELLEDRLYRASLGWFAHEP 1380

Query: 1381 EWYDVKHVNFAQSEAQSVSIFLHYLSSERGNSLHSDAKMRGRENGISLIDLNDHYHPVWG 1440
            EWYDVKHVNFAQS+AQSVSIFLHYLSSERGNSL SDAK+RGREN ISL+D ND YHPVWG
Sbjct: 1381 EWYDVKHVNFAQSQAQSVSIFLHYLSSERGNSLQSDAKIRGRENSISLVDSNDQYHPVWG 1440

Query: 1441 HLENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTPSRPKLTAEKWIEHARTAFSV 1500
            HLENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTPSRPKL+AEKWIEHARTAFSV
Sbjct: 1441 HLENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTPSRPKLSAEKWIEHARTAFSV 1500

Query: 1501 DPRIAFSMVSRFPTNAFLRVEMNQLVQLHILDIRSIPEALPYFVTPKAVDENSELLRQLP 1560
            DPRIAFSMVSRFPTNA LRVEMNQLVQLHILDIR+IPEALPYFVTPKAVDENSELLRQLP
Sbjct: 1501 DPRIAFSMVSRFPTNASLRVEMNQLVQLHILDIRTIPEALPYFVTPKAVDENSELLRQLP 1560

Query: 1561 HWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQALRYDEGRLVE 1620
            HWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQALRYDEGRLVE
Sbjct: 1561 HWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQALRYDEGRLVE 1620

Query: 1621 GYLLRAAKRSDIFAHILIWHLQGETSLPDSGKDVNSGKNGSFLALLPVVRQRIIDGFTPK 1680
            GYLLRAA+RSDIFAHILIWHLQGETSLPDSGKDVNSGKNGSFLALLPVVRQ IIDGFTPK
Sbjct: 1621 GYLLRAAQRSDIFAHILIWHLQGETSLPDSGKDVNSGKNGSFLALLPVVRQHIIDGFTPK 1680

Query: 1681 ALDLFKREFDFFDKVTSISGVLFPLPKDERRAGIRSELEKIEMEGEDLYLPTATNKLVCG 1740
            ALDLFKREFDFFDKVTSISGVLFPLPKDERRAGIR ELEKIEMEGEDLYLPTATNKLV G
Sbjct: 1681 ALDLFKREFDFFDKVTSISGVLFPLPKDERRAGIRRELEKIEMEGEDLYLPTATNKLVRG 1740

Query: 1741 IQVDSGIPLQSAAKVPIMVTFNVVDRDGDPNNIKPQACIFKVGDDCRQDVLALQVISLLR 1800
            IQVDSGIPLQSAAKVPIMVTFNVVDRDGDPNNIKPQACIFKVGDDCRQDVLALQVISLLR
Sbjct: 1741 IQVDSGIPLQSAAKVPIMVTFNVVDRDGDPNNIKPQACIFKVGDDCRQDVLALQVISLLR 1800

Query: 1801 DIFQAVGLNLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGPVGS 1860
            DIFQAVGLNLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGPVGS
Sbjct: 1801 DIFQAVGLNLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGPVGS 1860

Query: 1861 PNFEAARENFIVSSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFILETSPGGNMR 1920
            P+FEAARENFI+SSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFILETSPGGNMR
Sbjct: 1861 PSFEAARENFIISSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFILETSPGGNMR 1920

Query: 1921 FESAHFKLSHEMTQLLDPSGVMKSETWNLFVRLCVKGYLAARRHMHGIINTVLLMLDSGL 1980
            FESAHFKLSHEMTQLLDPSGVMKSETWNLFV LCVKGYLAARRHM GIINTVLLMLDSGL
Sbjct: 1921 FESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMDGIINTVLLMLDSGL 1980

Query: 1981 PCFSRGDPIGNLRKRFHPEMNDREAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK 2034
            PCFSRGDPIGNLRKRFHPEMNDREAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK
Sbjct: 1981 PCFSRGDPIGNLRKRFHPEMNDREAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK 2036

BLAST of Chy1G008030 vs. NCBI nr
Match: KAG7011695.1 (Phosphatidylinositol 4-kinase alpha 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 3821 bits (9909), Expect = 0.0
Identity = 1936/2037 (95.04%), Postives = 1982/2037 (97.30%), Query Frame = 0

Query: 1    MDALIELCDLIAQNPALFSEKLSWICSRCPPSEAILAGSPAISRSQLNAVLAVARLLSKC 60
            MDALIELCDLIAQNPALFSEKLSWICSRCPPSEAILAGSPAISRSQLNAVLAVARLLSKC
Sbjct: 1    MDALIELCDLIAQNPALFSEKLSWICSRCPPSEAILAGSPAISRSQLNAVLAVARLLSKC 60

Query: 61   PDSVGLRPKSVVLEFLRSIPLSFSLSFWPQSYGNDAIASFFNEFLNYTSKACALSTDFAT 120
            PDSVGLRPKSVVLEFLRSIPLSFSL+FWPQS+GNDAIASFF EFLNYTSKAC LSTDFA 
Sbjct: 61   PDSVGLRPKSVVLEFLRSIPLSFSLAFWPQSFGNDAIASFFTEFLNYTSKACELSTDFAM 120

Query: 121  EVSGFSSEVVLSAINDCSEGSAISRAFLVALSKSFLPIIPSDADKLVSCILDRFLISEAA 180
            EVSGF+SEVVLSAI+DCSEGSAISRAFLVALSKSFLPIIPSDADKLVSCILD FLISE  
Sbjct: 121  EVSGFTSEVVLSAISDCSEGSAISRAFLVALSKSFLPIIPSDADKLVSCILDLFLISEVV 180

Query: 181  PGTPREHNQANSEPSSSQSSPLSVSHQPSNGGLSPGNENGQVSGSLSSGASRITDDATSA 240
            PGTPRE  QANSEPSSSQSSPLSVSHQPSNGGLSPGNENGQVSGSLSSGASRITDDATSA
Sbjct: 181  PGTPREQIQANSEPSSSQSSPLSVSHQPSNGGLSPGNENGQVSGSLSSGASRITDDATSA 240

Query: 241  SSRGSGMMNGNSILWKSGLEQLGMNLGFSEGGGVAFVRQQVALFVDESIENLEKQEIAFK 300
            SSRGSGMMNGNSILWKSGL+QLGMNLG SEGG VAFVRQQVA F DESIENLEKQEIAFK
Sbjct: 241  SSRGSGMMNGNSILWKSGLDQLGMNLGTSEGG-VAFVRQQVAFFEDESIENLEKQEIAFK 300

Query: 301  LMTHILDNSSFDARLWEQMRALAKKQLQTLPTFLKIKKRDWSEQGSVLKARINTKLLVYQ 360
            LMTHILDNSSFDARLWEQMRALAKKQLQTLPTFLKIKKRDWSEQGSVLKARINTKLLVYQ
Sbjct: 301  LMTHILDNSSFDARLWEQMRALAKKQLQTLPTFLKIKKRDWSEQGSVLKARINTKLLVYQ 360

Query: 361  AAARLKMKTVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFSSLLTGL 420
            AAARLKM TVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELF SLLTGL
Sbjct: 361  AAARLKMTTVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFGSLLTGL 420

Query: 421  AQIAVARGGQPLRVLLIRLKPLVLTVCVQADTWGTNQGAMFESVLTTCCEIIESCWTKDR 480
            AQIAV+RGGQPLRVLLIRLKPL LTVC QADTWG+NQGAMFESVLTTCCEIIESCWTKDR
Sbjct: 421  AQIAVSRGGQPLRVLLIRLKPLALTVCAQADTWGSNQGAMFESVLTTCCEIIESCWTKDR 480

Query: 481  APVDTFIMGLATSIRDRNDSEEQDDKEKQGVPMQLNVIRLLAKMTVAVNKSEIVDMILPL 540
            APVDTFIMGLATSIRDRNDSEEQDDKEKQ VPMQLNVIRLLAKM+ AVNKSEIV+MILPL
Sbjct: 481  APVDTFIMGLATSIRDRNDSEEQDDKEKQAVPMQLNVIRLLAKMSAAVNKSEIVEMILPL 540

Query: 541  FIESLEEGDASTPGLLRLQLLDAVSRMATLGFEKSYRETIVLMTRSYLSKLSSIGSSESR 600
            FIESLEEGDASTPGLLRLQLLDAVSRMA+LGFEKSYRETIVL+TRSYLSKLSSIGSSESR
Sbjct: 541  FIESLEEGDASTPGLLRLQLLDAVSRMASLGFEKSYRETIVLLTRSYLSKLSSIGSSESR 600

Query: 601  TVAPEATTERVEILPAGFLHIASGLKSAKLRLEYRHRLLSLCSDVGLAAESKSGRSGANF 660
            TVAPEATTERVEILPAGFLHIASGLKSAKLRLEYRHRLL LCSDVGLAAESKSGRSGA+F
Sbjct: 601  TVAPEATTERVEILPAGFLHIASGLKSAKLRLEYRHRLLCLCSDVGLAAESKSGRSGADF 660

Query: 661  LGPLLPAVAEICSDFDPTMDIEPSLLKLFRNLWFYIALFGLAPPIQNSHLQTKSVSTMLN 720
            LGPLLPAVAEICSDFDPTM+IEPSLLKLFRNLWFYIALFGLAPPIQ S LQTKSVS+MLN
Sbjct: 661  LGPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQKSQLQTKSVSSMLN 720

Query: 721  SVGSTAIALQAVSGPYLWNTQWSSAVQLIARGTPPLVVSSVKWLEDELELNALHNPGSRR 780
            SVGSTAIALQAVSGPYLWNTQWSSAVQ IA+GTPPLVVSSVKWLEDELELNALHNPGSRR
Sbjct: 721  SVGSTAIALQAVSGPYLWNTQWSSAVQFIAQGTPPLVVSSVKWLEDELELNALHNPGSRR 780

Query: 781  GSGNEKAALAQRAALSSALGGRVDVAAMSTISGVKATYLLAVSFLEIIRFSSNGGILNGG 840
            GSGNEKAA+AQRAALS+ALGGRVDVAAMSTISGVKATYLLAVSFLEIIRFSSNGGILNGG
Sbjct: 781  GSGNEKAAVAQRAALSAALGGRVDVAAMSTISGVKATYLLAVSFLEIIRFSSNGGILNGG 840

Query: 841  SNVNASRSAFCCVFEYLKTPNLLPAVSQCLTAIVHRAFETAVLWLEDRISDTGNEAEVRD 900
            SNVNA+RSAF CVFEYLKTPNLLPAV+QCLTAIVHRAFETAV WLEDRIS+TGNEAEVRD
Sbjct: 841  SNVNAARSAFSCVFEYLKTPNLLPAVAQCLTAIVHRAFETAVSWLEDRISETGNEAEVRD 900

Query: 901  STLFAHTCYLIKSMSQRDEHVRDIAVNLLTQLRDKFPQVMWNSSCLDSLLFSMHNDAPST 960
            STL+AHTCYLIK MSQRDEHVRDI+VNLLTQLRDKFPQVMWNS+CLDSLLFSMHNDA ST
Sbjct: 901  STLYAHTCYLIKCMSQRDEHVRDISVNLLTQLRDKFPQVMWNSACLDSLLFSMHNDASST 960

Query: 961  VVTDPAWVVTVRSLYQRVVREWIVKSLSYAPCTCQGLLQEKLCKANTWQRAQHTPDVICL 1020
            VV+DPAWVVTVRSLYQRVVREWIVKSLSYAPCTCQGLLQEKLCKANTWQRAQHTPDVI L
Sbjct: 961  VVSDPAWVVTVRSLYQRVVREWIVKSLSYAPCTCQGLLQEKLCKANTWQRAQHTPDVISL 1020

Query: 1021 LSEIRIGTSKNEHWTGIQTANIPAVIAAAAAASGADLKLTEAFNLEVLSTGMVSATVKCN 1080
            LSEIRIGT KN++WTGIQTANIPAVI AAAAASGADLKLTEAFNLEVLSTGMVSATVKCN
Sbjct: 1021 LSEIRIGTGKNDYWTGIQTANIPAVITAAAAASGADLKLTEAFNLEVLSTGMVSATVKCN 1080

Query: 1081 HAGEIAGMRRLYNSIGGFQTGVAG---LGFGQGLQRLITGALPQQPQNEDDSFNGILIMK 1140
            HAGEIAGMRRLYNSIGGFQTGVAG   LGFGQGLQRLITG LPQQPQNEDDSFNGILIMK
Sbjct: 1081 HAGEIAGMRRLYNSIGGFQTGVAGPTGLGFGQGLQRLITGTLPQQPQNEDDSFNGILIMK 1140

Query: 1141 FVQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLASESKTNIEGFAQLIRLLCWCP 1200
            FVQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLASESKTNIEGFAQLIRLLCWCP
Sbjct: 1141 FVQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLASESKTNIEGFAQLIRLLCWCP 1200

Query: 1201 AYISTPDAIETGVFVWTWLVSAAPELGSFVLAELVDAWLWTVDTKRGLFASDVKYSGPAA 1260
            AYISTPDAIETGVF+WTWLVSAAPELGSFVLAELVDAWLWT+DTKRGLFAS+VKYSGPAA
Sbjct: 1201 AYISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRGLFASEVKYSGPAA 1260

Query: 1261 MLRPHLSPGEPEMQPEIDPVEQIIAHKIWLGFFIDRFEVVRHNSVEQLLLFGRLLQGSTK 1320
             LRPHLSPGEPE QPEIDPVEQIIAH+IWLGFFIDRFEVVRHNSVEQLLLFGRLLQGSTK
Sbjct: 1261 KLRPHLSPGEPESQPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQLLLFGRLLQGSTK 1320

Query: 1321 PPWNFSRHPAATGSFFTLMLLGLKFCSCQAQGNLQNFKTGLELLEDRIYRASLGWFAHEP 1380
            PPWNFSRHPAATGSFFTLMLLGLKF SCQ+QGNLQNFKTGLELLEDRIYRASLGWFAHEP
Sbjct: 1321 PPWNFSRHPAATGSFFTLMLLGLKFYSCQSQGNLQNFKTGLELLEDRIYRASLGWFAHEP 1380

Query: 1381 EWYDVKHVNFAQSEAQSVSIFLHYLSSERGNSLHSDAKMRGRENGISLIDLNDHYHPVWG 1440
            EWYDVKHVNFAQSEAQSVSIFLHYLSSERGNSL SD K RGRENGISL+D ND YHPVWG
Sbjct: 1381 EWYDVKHVNFAQSEAQSVSIFLHYLSSERGNSLQSDTKTRGRENGISLVDSNDQYHPVWG 1440

Query: 1441 HLENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTPSRPKLTAEKWIEHARTAFSV 1500
            HLENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTP RPKL+AEKWIEHARTAFSV
Sbjct: 1441 HLENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTPVRPKLSAEKWIEHARTAFSV 1500

Query: 1501 DPRIAFSMVSRFPTNAFLRVEMNQLVQLHILDIRSIPEALPYFVTPKAVDENSELLRQLP 1560
            DPRIAFSMVSRFPTN FLR E+ QLVQLHILDIRSIPEALPYF+TPKAVDENSELLRQLP
Sbjct: 1501 DPRIAFSMVSRFPTNTFLRAEVKQLVQLHILDIRSIPEALPYFITPKAVDENSELLRQLP 1560

Query: 1561 HWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQALRYDEGRLVE 1620
            HWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPE VTFFMPQLVQALRYDEGRLVE
Sbjct: 1561 HWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPENVTFFMPQLVQALRYDEGRLVE 1620

Query: 1621 GYLLRAAKRSDIFAHILIWHLQGETSLPDSGKDVNSGKNGSFLALLPVVRQRIIDGFTPK 1680
            GYLLRAA+RSDIFAHILIWHLQGETS+PDSGKD NSGKN SFLALLP+VR  IIDGFTPK
Sbjct: 1621 GYLLRAAQRSDIFAHILIWHLQGETSVPDSGKDANSGKNASFLALLPIVRHHIIDGFTPK 1680

Query: 1681 ALDLFKREFDFFDKVTSISGVLFPLPKDERRAGIRSELEKIEMEGEDLYLPTATNKLVCG 1740
            A DLF+REFDFFDKVTSISGVLFPLPKDERRAGIR ELEKIEMEGEDLYLPTATNKLV G
Sbjct: 1681 ARDLFEREFDFFDKVTSISGVLFPLPKDERRAGIRRELEKIEMEGEDLYLPTATNKLVRG 1740

Query: 1741 IQVDSGIPLQSAAKVPIMVTFNVVDRDGDPNNIKPQACIFKVGDDCRQDVLALQVISLLR 1800
            IQVDSGIPLQSAAKVPIM+TFNVVDRDGDPNNIKPQACIFKVGDDCRQDVLALQVI+LLR
Sbjct: 1741 IQVDSGIPLQSAAKVPIMITFNVVDRDGDPNNIKPQACIFKVGDDCRQDVLALQVIALLR 1800

Query: 1801 DIFQAVGLNLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGPVGS 1860
            DIFQAVGLNLYLFPYGVLPTGPG+GIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGPVGS
Sbjct: 1801 DIFQAVGLNLYLFPYGVLPTGPGKGIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGPVGS 1860

Query: 1861 PNFEAARENFIVSSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFILETSPGGNMR 1920
            P+FEAARENFI+SSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFILETSPGGNMR
Sbjct: 1861 PSFEAARENFIISSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFILETSPGGNMR 1920

Query: 1921 FESAHFKLSHEMTQLLDPSGVMKSETWNLFVRLCVKGYLAARRHMHGIINTVLLMLDSGL 1980
            FESAHFKLSHEMTQLLDPSGVMKSETWNLFV LCVKGYLAARRHM GIINTVLLMLDSGL
Sbjct: 1921 FESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMDGIINTVLLMLDSGL 1980

Query: 1981 PCFSRGDPIGNLRKRFHPEMNDREAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK 2034
            PCFSRGDPIGNLRKRFHPEM++REAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK
Sbjct: 1981 PCFSRGDPIGNLRKRFHPEMSEREAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK 2036

BLAST of Chy1G008030 vs. NCBI nr
Match: KAG6572023.1 (Phosphatidylinositol 4-kinase alpha 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 3821 bits (9908), Expect = 0.0
Identity = 1936/2037 (95.04%), Postives = 1981/2037 (97.25%), Query Frame = 0

Query: 1    MDALIELCDLIAQNPALFSEKLSWICSRCPPSEAILAGSPAISRSQLNAVLAVARLLSKC 60
            MDALIELCDLIAQNPALFSEKLSWICSRCPPSEAILAGSPAISRSQLNAVLAVARLLSKC
Sbjct: 1    MDALIELCDLIAQNPALFSEKLSWICSRCPPSEAILAGSPAISRSQLNAVLAVARLLSKC 60

Query: 61   PDSVGLRPKSVVLEFLRSIPLSFSLSFWPQSYGNDAIASFFNEFLNYTSKACALSTDFAT 120
            PDSVGLRPKSVVLEFLRSIPLSFSL+FWPQS+GNDAIASFF EFLNYTSKAC LSTDFA 
Sbjct: 61   PDSVGLRPKSVVLEFLRSIPLSFSLAFWPQSFGNDAIASFFTEFLNYTSKACELSTDFAM 120

Query: 121  EVSGFSSEVVLSAINDCSEGSAISRAFLVALSKSFLPIIPSDADKLVSCILDRFLISEAA 180
            EVSGF+SEVVLSAI+DCSEGSAISRAFLVALSKSFLPIIPSDADKLVSCILD FLISE  
Sbjct: 121  EVSGFTSEVVLSAISDCSEGSAISRAFLVALSKSFLPIIPSDADKLVSCILDLFLISEVV 180

Query: 181  PGTPREHNQANSEPSSSQSSPLSVSHQPSNGGLSPGNENGQVSGSLSSGASRITDDATSA 240
            PGTPRE  QANSEPSSSQSSPLSVSHQPSNGGLSPGNENGQVSGSLSSGASRITDDATSA
Sbjct: 181  PGTPREQIQANSEPSSSQSSPLSVSHQPSNGGLSPGNENGQVSGSLSSGASRITDDATSA 240

Query: 241  SSRGSGMMNGNSILWKSGLEQLGMNLGFSEGGGVAFVRQQVALFVDESIENLEKQEIAFK 300
            SSRGSGMMNGNSILWKSGL+QLGMNLG SEGG VAFVRQQVA F DESIENLEKQEIAFK
Sbjct: 241  SSRGSGMMNGNSILWKSGLDQLGMNLGTSEGG-VAFVRQQVAFFEDESIENLEKQEIAFK 300

Query: 301  LMTHILDNSSFDARLWEQMRALAKKQLQTLPTFLKIKKRDWSEQGSVLKARINTKLLVYQ 360
            LMTHILDNSSFDARLWEQMRALAKKQLQTLPTFLKIKKRDWSEQGSVLKARINTKLLVYQ
Sbjct: 301  LMTHILDNSSFDARLWEQMRALAKKQLQTLPTFLKIKKRDWSEQGSVLKARINTKLLVYQ 360

Query: 361  AAARLKMKTVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFSSLLTGL 420
            AAARLKM TVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELF SLLTGL
Sbjct: 361  AAARLKMTTVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFGSLLTGL 420

Query: 421  AQIAVARGGQPLRVLLIRLKPLVLTVCVQADTWGTNQGAMFESVLTTCCEIIESCWTKDR 480
            AQIAV RGGQPLRVLLIRLKPL LTVC QADTWG+NQGAMFESVLTTCCEIIESCWTKDR
Sbjct: 421  AQIAVTRGGQPLRVLLIRLKPLALTVCAQADTWGSNQGAMFESVLTTCCEIIESCWTKDR 480

Query: 481  APVDTFIMGLATSIRDRNDSEEQDDKEKQGVPMQLNVIRLLAKMTVAVNKSEIVDMILPL 540
            APVDTFIMGLATSIRDRNDSEEQDDKEKQ VPMQLNVIRLLAKM+ AVNKSEIV+MILPL
Sbjct: 481  APVDTFIMGLATSIRDRNDSEEQDDKEKQAVPMQLNVIRLLAKMSAAVNKSEIVEMILPL 540

Query: 541  FIESLEEGDASTPGLLRLQLLDAVSRMATLGFEKSYRETIVLMTRSYLSKLSSIGSSESR 600
            FIESLEEGDASTPGLLRLQLLDAVSRMA+LGFEKSYRETIVL+TRSYLSKLSSIGSSESR
Sbjct: 541  FIESLEEGDASTPGLLRLQLLDAVSRMASLGFEKSYRETIVLLTRSYLSKLSSIGSSESR 600

Query: 601  TVAPEATTERVEILPAGFLHIASGLKSAKLRLEYRHRLLSLCSDVGLAAESKSGRSGANF 660
            TVAPEATTERVEILPAGFLHIASGLKSAKLRLEYRHRLL LCSDVGLAAESKSGRSGA+F
Sbjct: 601  TVAPEATTERVEILPAGFLHIASGLKSAKLRLEYRHRLLCLCSDVGLAAESKSGRSGADF 660

Query: 661  LGPLLPAVAEICSDFDPTMDIEPSLLKLFRNLWFYIALFGLAPPIQNSHLQTKSVSTMLN 720
            LGPLLPAVAEICSDFDPTM+IEPSLLKLFRNLWFYIALFGLAPPIQ S LQTKSVS+MLN
Sbjct: 661  LGPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQKSQLQTKSVSSMLN 720

Query: 721  SVGSTAIALQAVSGPYLWNTQWSSAVQLIARGTPPLVVSSVKWLEDELELNALHNPGSRR 780
            SVGSTAIALQAVSGPYLWNTQWSSAVQ IA+GTPPLVVSSVKWLEDELELNALHNPGSRR
Sbjct: 721  SVGSTAIALQAVSGPYLWNTQWSSAVQFIAQGTPPLVVSSVKWLEDELELNALHNPGSRR 780

Query: 781  GSGNEKAALAQRAALSSALGGRVDVAAMSTISGVKATYLLAVSFLEIIRFSSNGGILNGG 840
            GSGNEKAA+AQRAALS+ALGGRVDVAAMSTISGVKATYLLAVSFLEIIRFSSNGGILNGG
Sbjct: 781  GSGNEKAAVAQRAALSAALGGRVDVAAMSTISGVKATYLLAVSFLEIIRFSSNGGILNGG 840

Query: 841  SNVNASRSAFCCVFEYLKTPNLLPAVSQCLTAIVHRAFETAVLWLEDRISDTGNEAEVRD 900
            SNVNA+RSAF CVFEYLKTPNLLPAV+QCLTAIVHRAFETAV WLEDRIS+TGNEAEVRD
Sbjct: 841  SNVNAARSAFSCVFEYLKTPNLLPAVAQCLTAIVHRAFETAVSWLEDRISETGNEAEVRD 900

Query: 901  STLFAHTCYLIKSMSQRDEHVRDIAVNLLTQLRDKFPQVMWNSSCLDSLLFSMHNDAPST 960
            STL+AHTCYLIK MSQRDEHVRDI+VNLLTQLRDKFPQVMWNS+CLDSLLFSMHNDA ST
Sbjct: 901  STLYAHTCYLIKCMSQRDEHVRDISVNLLTQLRDKFPQVMWNSACLDSLLFSMHNDASST 960

Query: 961  VVTDPAWVVTVRSLYQRVVREWIVKSLSYAPCTCQGLLQEKLCKANTWQRAQHTPDVICL 1020
            VV+DPAWVVTVRSLYQRVVREWIVKSLSYAPCTCQGLLQEKLCKANTWQRAQHTPDVI L
Sbjct: 961  VVSDPAWVVTVRSLYQRVVREWIVKSLSYAPCTCQGLLQEKLCKANTWQRAQHTPDVISL 1020

Query: 1021 LSEIRIGTSKNEHWTGIQTANIPAVIAAAAAASGADLKLTEAFNLEVLSTGMVSATVKCN 1080
            LSEIRIGT KN++WTGIQTANIPAVI AAAAASGADLKLTEAFNLEVLSTGMVSATVKCN
Sbjct: 1021 LSEIRIGTGKNDYWTGIQTANIPAVITAAAAASGADLKLTEAFNLEVLSTGMVSATVKCN 1080

Query: 1081 HAGEIAGMRRLYNSIGGFQTGVAG---LGFGQGLQRLITGALPQQPQNEDDSFNGILIMK 1140
            HAGEIAGMRRLYNSIGGFQTGVAG   LGFGQGLQRLITG LPQQPQNEDDSFNGILIMK
Sbjct: 1081 HAGEIAGMRRLYNSIGGFQTGVAGPTGLGFGQGLQRLITGTLPQQPQNEDDSFNGILIMK 1140

Query: 1141 FVQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLASESKTNIEGFAQLIRLLCWCP 1200
            FVQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLASESKTNIEGFAQLIRLLCWCP
Sbjct: 1141 FVQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLASESKTNIEGFAQLIRLLCWCP 1200

Query: 1201 AYISTPDAIETGVFVWTWLVSAAPELGSFVLAELVDAWLWTVDTKRGLFASDVKYSGPAA 1260
            AYISTPDAIETGVF+WTWLVSAAPELGSFVLAELVDAWLWT+DTKRGLFAS+VKYSGPAA
Sbjct: 1201 AYISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRGLFASEVKYSGPAA 1260

Query: 1261 MLRPHLSPGEPEMQPEIDPVEQIIAHKIWLGFFIDRFEVVRHNSVEQLLLFGRLLQGSTK 1320
             LRPHLSPGEPE QPEIDPVEQIIAH+IWLGFFIDRFEVVRHNSVEQLLLFGRLLQGSTK
Sbjct: 1261 KLRPHLSPGEPESQPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQLLLFGRLLQGSTK 1320

Query: 1321 PPWNFSRHPAATGSFFTLMLLGLKFCSCQAQGNLQNFKTGLELLEDRIYRASLGWFAHEP 1380
            PPWNFSRHPAATGSFFTLMLLGLKF SCQ+QGNLQNFKTGLELLEDRIYRASLGWFAHEP
Sbjct: 1321 PPWNFSRHPAATGSFFTLMLLGLKFYSCQSQGNLQNFKTGLELLEDRIYRASLGWFAHEP 1380

Query: 1381 EWYDVKHVNFAQSEAQSVSIFLHYLSSERGNSLHSDAKMRGRENGISLIDLNDHYHPVWG 1440
            EWYDVKHVNFAQSEAQSVSIFLHYLSSERGNSL SD K RGRENGISL+D ND YHPVWG
Sbjct: 1381 EWYDVKHVNFAQSEAQSVSIFLHYLSSERGNSLQSDTKTRGRENGISLVDSNDQYHPVWG 1440

Query: 1441 HLENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTPSRPKLTAEKWIEHARTAFSV 1500
            HLENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTP RPKL+AEKWIEHARTAFSV
Sbjct: 1441 HLENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTPVRPKLSAEKWIEHARTAFSV 1500

Query: 1501 DPRIAFSMVSRFPTNAFLRVEMNQLVQLHILDIRSIPEALPYFVTPKAVDENSELLRQLP 1560
            DPRIAFSMVSRFPTN FLR E+ QLVQLHILDIRSIPEALPYF+TPKAVDENSELLRQLP
Sbjct: 1501 DPRIAFSMVSRFPTNTFLRAEVKQLVQLHILDIRSIPEALPYFITPKAVDENSELLRQLP 1560

Query: 1561 HWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQALRYDEGRLVE 1620
            HWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPE VTFFMPQLVQALRYDEGRLVE
Sbjct: 1561 HWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPENVTFFMPQLVQALRYDEGRLVE 1620

Query: 1621 GYLLRAAKRSDIFAHILIWHLQGETSLPDSGKDVNSGKNGSFLALLPVVRQRIIDGFTPK 1680
            GYLLRAA+RSDIFAHILIWHLQGETS+PDSGKD NSGKN SFLALLP+VR  IIDGFTPK
Sbjct: 1621 GYLLRAAQRSDIFAHILIWHLQGETSVPDSGKDANSGKNASFLALLPIVRHHIIDGFTPK 1680

Query: 1681 ALDLFKREFDFFDKVTSISGVLFPLPKDERRAGIRSELEKIEMEGEDLYLPTATNKLVCG 1740
            A DLF+REFDFFDKVTSISGVLFPLPKDERRAGIR ELEKIEMEGEDLYLPTATNKLV G
Sbjct: 1681 ARDLFEREFDFFDKVTSISGVLFPLPKDERRAGIRRELEKIEMEGEDLYLPTATNKLVRG 1740

Query: 1741 IQVDSGIPLQSAAKVPIMVTFNVVDRDGDPNNIKPQACIFKVGDDCRQDVLALQVISLLR 1800
            IQVDSGIPLQSAAKVPIM+TFNVVDRDGDPNNIKPQACIFKVGDDCRQDVLALQVI+LLR
Sbjct: 1741 IQVDSGIPLQSAAKVPIMITFNVVDRDGDPNNIKPQACIFKVGDDCRQDVLALQVIALLR 1800

Query: 1801 DIFQAVGLNLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGPVGS 1860
            DIFQAVGLNLYLFPYGVLPTGPG+GIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGPVGS
Sbjct: 1801 DIFQAVGLNLYLFPYGVLPTGPGKGIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGPVGS 1860

Query: 1861 PNFEAARENFIVSSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFILETSPGGNMR 1920
            P+FEAARENFI+SSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFILETSPGGNMR
Sbjct: 1861 PSFEAARENFIISSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFILETSPGGNMR 1920

Query: 1921 FESAHFKLSHEMTQLLDPSGVMKSETWNLFVRLCVKGYLAARRHMHGIINTVLLMLDSGL 1980
            FESAHFKLSHEMTQLLDPSGVMKSETWNLFV LCVKGYLAARRHM GIINTVLLMLDSGL
Sbjct: 1921 FESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMDGIINTVLLMLDSGL 1980

Query: 1981 PCFSRGDPIGNLRKRFHPEMNDREAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK 2034
            PCFSRGDPIGNLRKRFHPEM++REAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK
Sbjct: 1981 PCFSRGDPIGNLRKRFHPEMSEREAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK 2036

BLAST of Chy1G008030 vs. TAIR 10
Match: AT1G49340.2 (Phosphatidylinositol 3- and 4-kinase family protein )

HSP 1 Score: 2989.5 bits (7749), Expect = 0.0e+00
Identity = 1521/2050 (74.20%), Postives = 1742/2050 (84.98%), Query Frame = 0

Query: 1    MDALIELCDLIAQNPALFSEKLSWICSRCPPSEAILAGSPAISRSQLNAVLAVARLLSKC 60
            M+AL ELCD+IA+NP  FSEKL+WIC RCP +E +LA SP +SRS LNAVLAVAR++SK 
Sbjct: 1    MEALTELCDIIAKNPKQFSEKLAWICGRCPQTEWLLAESPRVSRSHLNAVLAVARIISKN 60

Query: 61   PDSVGLRPKSVVLEFLRSIPLSFSLSFWPQSYGNDAIASFFNEFLNYTSKACALSTDFAT 120
            P+S+  R KSVV EFL +IP SF  SFWP S+ +  I+SF+ +FL+Y S A  LS +F T
Sbjct: 61   PESIDNRAKSVVNEFLSAIPASFRRSFWPHSFPSQLISSFYCDFLSYLSCAADLSPEFGT 120

Query: 121  EVSGFSSEVVLSAI----NDCSEGSAISRAFLVALSKSFLPIIPSDADKLVSCILDRFLI 180
            EV+ F+ EVV++AI     D     AIS+AFLVALS+ F  I+ SD DKL++ +LD+F++
Sbjct: 121  EVARFTGEVVIAAIAPSSGDSDGDPAISKAFLVALSQHFPSILQSDGDKLITMLLDQFVL 180

Query: 181  SEAAPGTPREHNQANS-----EPSSSQSSPLSVSHQPSNGG--LSPGNENGQVSGSLSSG 240
            +  AP +P+E  Q NS     + SSSQ SP+S +  PS      SPG+E      +LSS 
Sbjct: 181  NR-APASPKEQRQQNSANSETDTSSSQGSPISTNRYPSGKTEMASPGDEVASHGSNLSS- 240

Query: 241  ASRITDDATSASSRGSGMMNGNSILWKSGLEQLGMNLGFSEGGGVA--FVRQQVALFVDE 300
                        S  S +MNG SI+WKSG++QL  + GFSEG G A    RQQVA F DE
Sbjct: 241  -----------KSSSSVVMNGGSIVWKSGVDQL--SFGFSEGSGGANPVFRQQVASFEDE 300

Query: 301  SIENLEKQEIAFKLMTHILDNSSFDARLWEQMRALAKKQLQTLPTFLKIKKRDWSEQGSV 360
            SIE+LEKQEIAF+L+THILD    D++L +Q+R +AK+QLQ++  FLK +KRDW+EQG V
Sbjct: 301  SIESLEKQEIAFRLITHILDKVKIDSKLQDQVRFIAKRQLQSMSAFLKSRKRDWNEQGQV 360

Query: 361  LKARINTKLLVYQAAARLKMKTVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLR 420
            LK R+N KL VYQAAA++K+K++ SL+ DGK +K+L+ ET ALL+DAA+ACL SVWRK++
Sbjct: 361  LKTRVNAKLSVYQAAAKMKIKSLVSLETDGKTSKRLVLETLALLLDAADACLTSVWRKMK 420

Query: 421  ICEELFSSLLTGLAQIAVARGGQPLRVLLIRLKPLVLTVCVQADTWGTNQGAMFESVLTT 480
             CEELF SLL+G+A+IAVARGGQPLRVLLIRLKPLVL VC   D     QGAM ES+  T
Sbjct: 421  ACEELFDSLLSGIAKIAVARGGQPLRVLLIRLKPLVLAVCALPD-----QGAMLESIFKT 480

Query: 481  CCEIIESCWTKDRAPVDTFIMGLATSIRDRNDSEEQDDKEKQGVPMQLNVIRLLAKMTVA 540
             C IIES W KDRAPVD FIMGLA+SIR+RND EEQ D+EKQ   +QLNVIRLLA + VA
Sbjct: 481  SCVIIESAWAKDRAPVDNFIMGLASSIRERNDYEEQVDREKQVPAVQLNVIRLLADLNVA 540

Query: 541  VNKSEIVDMILPLFIESLEEGDASTPGLLRLQLLDAVSRMATLGFEKSYRETIVLMTRSY 600
            V K E+ DMILPLFIESLEEGDASTP  LRLQLLDAVSR+ATLGF+KSYRET+VLMTRSY
Sbjct: 541  VKKPEVADMILPLFIESLEEGDASTPSFLRLQLLDAVSRIATLGFDKSYRETVVLMTRSY 600

Query: 601  LSKLSSIGSSESRTVAPEATTERVEILPAGFLHIASGLKSAKLRLEYRHRLLSLCSDVGL 660
            LSKLSS+GS ES+T APEATTERVE LPAGFL IASGL   KLR +YRHRLLSLCSDVGL
Sbjct: 601  LSKLSSVGSVESKTSAPEATTERVETLPAGFLTIASGLMDTKLRSDYRHRLLSLCSDVGL 660

Query: 661  AAESKSGRSGANFLGPLLPAVAEICSDFDPTMDIEPSLLKLFRNLWFYIALFGLAPPIQN 720
            AAESKSG SG +FLGPLLPAVAEICSDFDPTMD+EPSLLKLFRNLWFYIALFGLAPPI  
Sbjct: 661  AAESKSGGSGVDFLGPLLPAVAEICSDFDPTMDVEPSLLKLFRNLWFYIALFGLAPPIVK 720

Query: 721  SHL-QTKSVSTMLNSVGS-TAIALQAVSGPYLWNTQWSSAVQLIARGTPPLVVSSVKWLE 780
            +     KS S  +NSVGS +A ALQAV GPY+W+ QW+ AVQ IA+GTPPLVVSSVKWLE
Sbjct: 721  TPTPPLKSTSNSVNSVGSMSATALQAVGGPYMWDNQWALAVQRIAQGTPPLVVSSVKWLE 780

Query: 781  DELELNALHNPGSRRGSGNEKAALAQRAALSSALGGRVDVAAMSTISGVKATYLLAVSFL 840
            DELELNALHNPGSRRG+GNEK A  QR ALS+ALGGRVDVAAM+TISGVKATYLLAV+FL
Sbjct: 781  DELELNALHNPGSRRGNGNEKVASTQRLALSTALGGRVDVAAMNTISGVKATYLLAVAFL 840

Query: 841  EIIRFSSNGGILNGGSNVNASRSAFCCVFEYLKTPNLLPAVSQCLTAIVHRAFETAVLWL 900
            EIIRF SNGGILNG S+V+ASRSAF CVFEYLKTPNL PAVSQCLTAIVHRAFETAV WL
Sbjct: 841  EIIRFISNGGILNGESSVSASRSAFSCVFEYLKTPNLTPAVSQCLTAIVHRAFETAVSWL 900

Query: 901  EDRISDTGNEAEVRDSTLFAHTCYLIKSMSQRDEHVRDIAVNLLTQLRDKFPQVMWNSSC 960
            EDRIS TG +A  R+ T +AH C+LIKSMSQRDEHVRDI+VNLLTQLRDKFPQV+W+SSC
Sbjct: 901  EDRISLTGKDARNRELTTYAHACFLIKSMSQRDEHVRDISVNLLTQLRDKFPQVLWHSSC 960

Query: 961  LDSLLFSMHNDAPSTVVTDPAWVVTVRSLYQRVVREWIVKSLSYAPCTCQGLLQEKLCKA 1020
            LDSLLFS+H++ PSTVV DPAW   VRSLYQ+VVREWI+ SLSYAPCT QGLLQ+KLCKA
Sbjct: 961  LDSLLFSVHDNTPSTVVNDPAWTAAVRSLYQKVVREWIIISLSYAPCTSQGLLQDKLCKA 1020

Query: 1021 NTWQRAQHTPDVICLLSEIRIGTSKNEHWTGIQTANIPAVIAAAAAASGADLKLTEAFNL 1080
            NTWQRAQ T DV+ LLSEI+IGT KNE W+GI+TANIPAV+AAAAAASGA+LK++EAFNL
Sbjct: 1021 NTWQRAQTTTDVVSLLSEIKIGTGKNELWSGIRTANIPAVMAAAAAASGANLKVSEAFNL 1080

Query: 1081 EVLSTGMVSATVKCNHAGEIAGMRRLYNSIGGFQTGVAGLGFGQGLQRLITGALPQQPQN 1140
            EVL TG+VSATVKCNHAGEIAGMRRLYNSIGGFQ+G    GFG GLQRLI+GA  Q PQ 
Sbjct: 1081 EVLGTGVVSATVKCNHAGEIAGMRRLYNSIGGFQSGSTPSGFGGGLQRLISGAFSQAPQP 1140

Query: 1141 EDDSFNGILIMKFVQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLASESKTNIEG 1200
            EDDSFN +LI +FV+ LQQFV+ AEKG  ++K +FRETCSQATALLLSNL  ESKTN+EG
Sbjct: 1141 EDDSFNEMLIARFVRLLQQFVNTAEKGGEVEKSQFRETCSQATALLLSNLGGESKTNVEG 1200

Query: 1201 FAQLIRLLCWCPAYISTPDAIETGVFVWTWLVSAAPELGSFVLAELVDAWLWTVDTKRGL 1260
            F+QL+RLLCWCPAYISTPDA+ETG+F+WTWLVSAAP+L S VLAELVDAW+WT+DTKRGL
Sbjct: 1201 FSQLLRLLCWCPAYISTPDAMETGIFIWTWLVSAAPQLVSLVLAELVDAWIWTIDTKRGL 1260

Query: 1261 FASDVKYSGPAAMLRPHLSPGEPEMQPEIDPVEQIIAHKIWLGFFIDRFEVVRHNSVEQL 1320
            FASDV+YSGPAA LRPHLSPGEPE  PE DPV+QI+AH++WLGF IDRFEVVRHNS EQL
Sbjct: 1261 FASDVRYSGPAAKLRPHLSPGEPEDPPESDPVDQIVAHRLWLGFLIDRFEVVRHNSAEQL 1320

Query: 1321 LLFGRLLQGSTKPPWNFSRHPAATGSFFTLMLLGLKFCSCQAQGNLQNFKTGLELLEDRI 1380
            LL GR+LQ ST   W F+RHPAA G+FF+LMLLGLKFCSCQ QGN+Q F++GL+LLEDRI
Sbjct: 1321 LLLGRMLQRSTDLEWCFTRHPAAAGTFFSLMLLGLKFCSCQTQGNMQKFRSGLQLLEDRI 1380

Query: 1381 YRASLGWFAHEPEWYDVKHVNFAQSEAQSVSIFLHYLSSERGNSLHSDAKMRGRENGISL 1440
            YR SLGWFAH+PEWYDV   NF  SEA SVS+F+H+LS+E   S  SD+K + RE+G +L
Sbjct: 1381 YRTSLGWFAHQPEWYDVNIPNFCHSEALSVSVFVHFLSNELSESSQSDSKGKPRESG-NL 1440

Query: 1441 IDLNDHYHPVWGHLENYAVGREKRRQLLLMLCQHEADRLEVWAQP-NIKESTPSRPKLTA 1500
            ID+ D YHPVWG ++NY +G+EKR+QLLLMLCQHEADRL+VWAQP + K+S  SR K+++
Sbjct: 1441 IDVTDQYHPVWGEMDNYTLGKEKRKQLLLMLCQHEADRLDVWAQPISSKDSPYSRLKISS 1500

Query: 1501 EKWIEHARTAFSVDPRIAFSMVSRFPTNAFLRVEMNQLVQLHILDIRSIPEALPYFVTPK 1560
            EKW E+A+TAFSVDPRIA S+ SRFP NA ++ E+ QLVQ +I+D+R+IPEALPYFVTPK
Sbjct: 1501 EKWTEYAKTAFSVDPRIALSVASRFPANASVKSEVTQLVQTNIVDLRTIPEALPYFVTPK 1560

Query: 1561 AVDENSELLRQLPHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQL 1620
             V+ENS LL+QLPHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPE+VTFFMPQL
Sbjct: 1561 NVEENSVLLQQLPHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPERVTFFMPQL 1620

Query: 1621 VQALRYDEGRLVEGYLLRAAKRSDIFAHILIWHLQGETSLPDSGKDVNSGKNGSFLALLP 1680
            VQ+LRYD+GRLVEGYLLRA +RSDIFAHILIWHLQGE  + ++ KD +  KN +F  +LP
Sbjct: 1621 VQSLRYDDGRLVEGYLLRATQRSDIFAHILIWHLQGE-DVQETPKDGSIDKNAAFQEILP 1680

Query: 1681 VVRQRIIDGFTPKALDLFKREFDFFDKVTSISGVLFPLPKDERRAGIRSELEKIEMEGED 1740
             VRQ IIDGF+P ALD+F REFDFFDKVTSISGVLFPLPK+ERRAGIR ELEKIEM+G+D
Sbjct: 1681 QVRQHIIDGFSPNALDMFTREFDFFDKVTSISGVLFPLPKEERRAGIRRELEKIEMQGDD 1740

Query: 1741 LYLPTATNKLVCGIQVDSGIPLQSAAKVPIMVTFNVVDRDGDPNNIKPQACIFKVGDDCR 1800
            LYLPTA NKLV GI+VDSGIPLQSAAKVPIM+TFNV+DRDGD +++KPQACIFKVGDDCR
Sbjct: 1741 LYLPTAPNKLVRGIRVDSGIPLQSAAKVPIMITFNVIDRDGDHSDVKPQACIFKVGDDCR 1800

Query: 1801 QDVLALQVISLLRDIFQAVGLNLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGL 1860
            QDVLALQVISLLRDIFQA GLNLYLFPYGVLPTG  RGIIEVVPNTRSRSQMGETTDGGL
Sbjct: 1801 QDVLALQVISLLRDIFQAAGLNLYLFPYGVLPTGAERGIIEVVPNTRSRSQMGETTDGGL 1860

Query: 1861 YEIFQQDYGPVGSPNFEAARENFIVSSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDF 1920
            YEIFQQDYGPVGS  FE ARENF++SSAGYAVASLLLQPKDRHNGNLLFD+VGRLVHIDF
Sbjct: 1861 YEIFQQDYGPVGSTTFETARENFLISSAGYAVASLLLQPKDRHNGNLLFDDVGRLVHIDF 1920

Query: 1921 GFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSETWNLFVRLCVKGYLAARRHMHG 1980
            GFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKS+TW+ FV LCVKGYLAARR M G
Sbjct: 1921 GFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSKTWHQFVSLCVKGYLAARRQMDG 1980

Query: 1981 IINTVLLMLDSGLPCFSRGDPIGNLRKRFHPEMNDREAANFMIRVCTDAYNKWTTAGYDL 2035
            II+TV +ML+SGLPCFSRGDPIGNLRKRFHPEM++REAA+FMI VCTDAYNKWTTAGYDL
Sbjct: 1981 IISTVQMMLESGLPCFSRGDPIGNLRKRFHPEMSEREAAHFMIHVCTDAYNKWTTAGYDL 2028

BLAST of Chy1G008030 vs. TAIR 10
Match: AT1G49340.1 (Phosphatidylinositol 3- and 4-kinase family protein )

HSP 1 Score: 2989.5 bits (7749), Expect = 0.0e+00
Identity = 1521/2050 (74.20%), Postives = 1742/2050 (84.98%), Query Frame = 0

Query: 1    MDALIELCDLIAQNPALFSEKLSWICSRCPPSEAILAGSPAISRSQLNAVLAVARLLSKC 60
            M+AL ELCD+IA+NP  FSEKL+WIC RCP +E +LA SP +SRS LNAVLAVAR++SK 
Sbjct: 1    MEALTELCDIIAKNPKQFSEKLAWICGRCPQTEWLLAESPRVSRSHLNAVLAVARIISKN 60

Query: 61   PDSVGLRPKSVVLEFLRSIPLSFSLSFWPQSYGNDAIASFFNEFLNYTSKACALSTDFAT 120
            P+S+  R KSVV EFL +IP SF  SFWP S+ +  I+SF+ +FL+Y S A  LS +F T
Sbjct: 61   PESIDNRAKSVVNEFLSAIPASFRRSFWPHSFPSQLISSFYCDFLSYLSCAADLSPEFGT 120

Query: 121  EVSGFSSEVVLSAI----NDCSEGSAISRAFLVALSKSFLPIIPSDADKLVSCILDRFLI 180
            EV+ F+ EVV++AI     D     AIS+AFLVALS+ F  I+ SD DKL++ +LD+F++
Sbjct: 121  EVARFTGEVVIAAIAPSSGDSDGDPAISKAFLVALSQHFPSILQSDGDKLITMLLDQFVL 180

Query: 181  SEAAPGTPREHNQANS-----EPSSSQSSPLSVSHQPSNGG--LSPGNENGQVSGSLSSG 240
            +  AP +P+E  Q NS     + SSSQ SP+S +  PS      SPG+E      +LSS 
Sbjct: 181  NR-APASPKEQRQQNSANSETDTSSSQGSPISTNRYPSGKTEMASPGDEVASHGSNLSS- 240

Query: 241  ASRITDDATSASSRGSGMMNGNSILWKSGLEQLGMNLGFSEGGGVA--FVRQQVALFVDE 300
                        S  S +MNG SI+WKSG++QL  + GFSEG G A    RQQVA F DE
Sbjct: 241  -----------KSSSSVVMNGGSIVWKSGVDQL--SFGFSEGSGGANPVFRQQVASFEDE 300

Query: 301  SIENLEKQEIAFKLMTHILDNSSFDARLWEQMRALAKKQLQTLPTFLKIKKRDWSEQGSV 360
            SIE+LEKQEIAF+L+THILD    D++L +Q+R +AK+QLQ++  FLK +KRDW+EQG V
Sbjct: 301  SIESLEKQEIAFRLITHILDKVKIDSKLQDQVRFIAKRQLQSMSAFLKSRKRDWNEQGQV 360

Query: 361  LKARINTKLLVYQAAARLKMKTVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLR 420
            LK R+N KL VYQAAA++K+K++ SL+ DGK +K+L+ ET ALL+DAA+ACL SVWRK++
Sbjct: 361  LKTRVNAKLSVYQAAAKMKIKSLVSLETDGKTSKRLVLETLALLLDAADACLTSVWRKMK 420

Query: 421  ICEELFSSLLTGLAQIAVARGGQPLRVLLIRLKPLVLTVCVQADTWGTNQGAMFESVLTT 480
             CEELF SLL+G+A+IAVARGGQPLRVLLIRLKPLVL VC   D     QGAM ES+  T
Sbjct: 421  ACEELFDSLLSGIAKIAVARGGQPLRVLLIRLKPLVLAVCALPD-----QGAMLESIFKT 480

Query: 481  CCEIIESCWTKDRAPVDTFIMGLATSIRDRNDSEEQDDKEKQGVPMQLNVIRLLAKMTVA 540
             C IIES W KDRAPVD FIMGLA+SIR+RND EEQ D+EKQ   +QLNVIRLLA + VA
Sbjct: 481  SCVIIESAWAKDRAPVDNFIMGLASSIRERNDYEEQVDREKQVPAVQLNVIRLLADLNVA 540

Query: 541  VNKSEIVDMILPLFIESLEEGDASTPGLLRLQLLDAVSRMATLGFEKSYRETIVLMTRSY 600
            V K E+ DMILPLFIESLEEGDASTP  LRLQLLDAVSR+ATLGF+KSYRET+VLMTRSY
Sbjct: 541  VKKPEVADMILPLFIESLEEGDASTPSFLRLQLLDAVSRIATLGFDKSYRETVVLMTRSY 600

Query: 601  LSKLSSIGSSESRTVAPEATTERVEILPAGFLHIASGLKSAKLRLEYRHRLLSLCSDVGL 660
            LSKLSS+GS ES+T APEATTERVE LPAGFL IASGL   KLR +YRHRLLSLCSDVGL
Sbjct: 601  LSKLSSVGSVESKTSAPEATTERVETLPAGFLTIASGLMDTKLRSDYRHRLLSLCSDVGL 660

Query: 661  AAESKSGRSGANFLGPLLPAVAEICSDFDPTMDIEPSLLKLFRNLWFYIALFGLAPPIQN 720
            AAESKSG SG +FLGPLLPAVAEICSDFDPTMD+EPSLLKLFRNLWFYIALFGLAPPI  
Sbjct: 661  AAESKSGGSGVDFLGPLLPAVAEICSDFDPTMDVEPSLLKLFRNLWFYIALFGLAPPIVK 720

Query: 721  SHL-QTKSVSTMLNSVGS-TAIALQAVSGPYLWNTQWSSAVQLIARGTPPLVVSSVKWLE 780
            +     KS S  +NSVGS +A ALQAV GPY+W+ QW+ AVQ IA+GTPPLVVSSVKWLE
Sbjct: 721  TPTPPLKSTSNSVNSVGSMSATALQAVGGPYMWDNQWALAVQRIAQGTPPLVVSSVKWLE 780

Query: 781  DELELNALHNPGSRRGSGNEKAALAQRAALSSALGGRVDVAAMSTISGVKATYLLAVSFL 840
            DELELNALHNPGSRRG+GNEK A  QR ALS+ALGGRVDVAAM+TISGVKATYLLAV+FL
Sbjct: 781  DELELNALHNPGSRRGNGNEKVASTQRLALSTALGGRVDVAAMNTISGVKATYLLAVAFL 840

Query: 841  EIIRFSSNGGILNGGSNVNASRSAFCCVFEYLKTPNLLPAVSQCLTAIVHRAFETAVLWL 900
            EIIRF SNGGILNG S+V+ASRSAF CVFEYLKTPNL PAVSQCLTAIVHRAFETAV WL
Sbjct: 841  EIIRFISNGGILNGESSVSASRSAFSCVFEYLKTPNLTPAVSQCLTAIVHRAFETAVSWL 900

Query: 901  EDRISDTGNEAEVRDSTLFAHTCYLIKSMSQRDEHVRDIAVNLLTQLRDKFPQVMWNSSC 960
            EDRIS TG +A  R+ T +AH C+LIKSMSQRDEHVRDI+VNLLTQLRDKFPQV+W+SSC
Sbjct: 901  EDRISLTGKDARNRELTTYAHACFLIKSMSQRDEHVRDISVNLLTQLRDKFPQVLWHSSC 960

Query: 961  LDSLLFSMHNDAPSTVVTDPAWVVTVRSLYQRVVREWIVKSLSYAPCTCQGLLQEKLCKA 1020
            LDSLLFS+H++ PSTVV DPAW   VRSLYQ+VVREWI+ SLSYAPCT QGLLQ+KLCKA
Sbjct: 961  LDSLLFSVHDNTPSTVVNDPAWTAAVRSLYQKVVREWIIISLSYAPCTSQGLLQDKLCKA 1020

Query: 1021 NTWQRAQHTPDVICLLSEIRIGTSKNEHWTGIQTANIPAVIAAAAAASGADLKLTEAFNL 1080
            NTWQRAQ T DV+ LLSEI+IGT KNE W+GI+TANIPAV+AAAAAASGA+LK++EAFNL
Sbjct: 1021 NTWQRAQTTTDVVSLLSEIKIGTGKNELWSGIRTANIPAVMAAAAAASGANLKVSEAFNL 1080

Query: 1081 EVLSTGMVSATVKCNHAGEIAGMRRLYNSIGGFQTGVAGLGFGQGLQRLITGALPQQPQN 1140
            EVL TG+VSATVKCNHAGEIAGMRRLYNSIGGFQ+G    GFG GLQRLI+GA  Q PQ 
Sbjct: 1081 EVLGTGVVSATVKCNHAGEIAGMRRLYNSIGGFQSGSTPSGFGGGLQRLISGAFSQAPQP 1140

Query: 1141 EDDSFNGILIMKFVQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLASESKTNIEG 1200
            EDDSFN +LI +FV+ LQQFV+ AEKG  ++K +FRETCSQATALLLSNL  ESKTN+EG
Sbjct: 1141 EDDSFNEMLIARFVRLLQQFVNTAEKGGEVEKSQFRETCSQATALLLSNLGGESKTNVEG 1200

Query: 1201 FAQLIRLLCWCPAYISTPDAIETGVFVWTWLVSAAPELGSFVLAELVDAWLWTVDTKRGL 1260
            F+QL+RLLCWCPAYISTPDA+ETG+F+WTWLVSAAP+L S VLAELVDAW+WT+DTKRGL
Sbjct: 1201 FSQLLRLLCWCPAYISTPDAMETGIFIWTWLVSAAPQLVSLVLAELVDAWIWTIDTKRGL 1260

Query: 1261 FASDVKYSGPAAMLRPHLSPGEPEMQPEIDPVEQIIAHKIWLGFFIDRFEVVRHNSVEQL 1320
            FASDV+YSGPAA LRPHLSPGEPE  PE DPV+QI+AH++WLGF IDRFEVVRHNS EQL
Sbjct: 1261 FASDVRYSGPAAKLRPHLSPGEPEDPPESDPVDQIVAHRLWLGFLIDRFEVVRHNSAEQL 1320

Query: 1321 LLFGRLLQGSTKPPWNFSRHPAATGSFFTLMLLGLKFCSCQAQGNLQNFKTGLELLEDRI 1380
            LL GR+LQ ST   W F+RHPAA G+FF+LMLLGLKFCSCQ QGN+Q F++GL+LLEDRI
Sbjct: 1321 LLLGRMLQRSTDLEWCFTRHPAAAGTFFSLMLLGLKFCSCQTQGNMQKFRSGLQLLEDRI 1380

Query: 1381 YRASLGWFAHEPEWYDVKHVNFAQSEAQSVSIFLHYLSSERGNSLHSDAKMRGRENGISL 1440
            YR SLGWFAH+PEWYDV   NF  SEA SVS+F+H+LS+E   S  SD+K + RE+G +L
Sbjct: 1381 YRTSLGWFAHQPEWYDVNIPNFCHSEALSVSVFVHFLSNELSESSQSDSKGKPRESG-NL 1440

Query: 1441 IDLNDHYHPVWGHLENYAVGREKRRQLLLMLCQHEADRLEVWAQP-NIKESTPSRPKLTA 1500
            ID+ D YHPVWG ++NY +G+EKR+QLLLMLCQHEADRL+VWAQP + K+S  SR K+++
Sbjct: 1441 IDVTDQYHPVWGEMDNYTLGKEKRKQLLLMLCQHEADRLDVWAQPISSKDSPYSRLKISS 1500

Query: 1501 EKWIEHARTAFSVDPRIAFSMVSRFPTNAFLRVEMNQLVQLHILDIRSIPEALPYFVTPK 1560
            EKW E+A+TAFSVDPRIA S+ SRFP NA ++ E+ QLVQ +I+D+R+IPEALPYFVTPK
Sbjct: 1501 EKWTEYAKTAFSVDPRIALSVASRFPANASVKSEVTQLVQTNIVDLRTIPEALPYFVTPK 1560

Query: 1561 AVDENSELLRQLPHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQL 1620
             V+ENS LL+QLPHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPE+VTFFMPQL
Sbjct: 1561 NVEENSVLLQQLPHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPERVTFFMPQL 1620

Query: 1621 VQALRYDEGRLVEGYLLRAAKRSDIFAHILIWHLQGETSLPDSGKDVNSGKNGSFLALLP 1680
            VQ+LRYD+GRLVEGYLLRA +RSDIFAHILIWHLQGE  + ++ KD +  KN +F  +LP
Sbjct: 1621 VQSLRYDDGRLVEGYLLRATQRSDIFAHILIWHLQGE-DVQETPKDGSIDKNAAFQEILP 1680

Query: 1681 VVRQRIIDGFTPKALDLFKREFDFFDKVTSISGVLFPLPKDERRAGIRSELEKIEMEGED 1740
             VRQ IIDGF+P ALD+F REFDFFDKVTSISGVLFPLPK+ERRAGIR ELEKIEM+G+D
Sbjct: 1681 QVRQHIIDGFSPNALDMFTREFDFFDKVTSISGVLFPLPKEERRAGIRRELEKIEMQGDD 1740

Query: 1741 LYLPTATNKLVCGIQVDSGIPLQSAAKVPIMVTFNVVDRDGDPNNIKPQACIFKVGDDCR 1800
            LYLPTA NKLV GI+VDSGIPLQSAAKVPIM+TFNV+DRDGD +++KPQACIFKVGDDCR
Sbjct: 1741 LYLPTAPNKLVRGIRVDSGIPLQSAAKVPIMITFNVIDRDGDHSDVKPQACIFKVGDDCR 1800

Query: 1801 QDVLALQVISLLRDIFQAVGLNLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGL 1860
            QDVLALQVISLLRDIFQA GLNLYLFPYGVLPTG  RGIIEVVPNTRSRSQMGETTDGGL
Sbjct: 1801 QDVLALQVISLLRDIFQAAGLNLYLFPYGVLPTGAERGIIEVVPNTRSRSQMGETTDGGL 1860

Query: 1861 YEIFQQDYGPVGSPNFEAARENFIVSSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDF 1920
            YEIFQQDYGPVGS  FE ARENF++SSAGYAVASLLLQPKDRHNGNLLFD+VGRLVHIDF
Sbjct: 1861 YEIFQQDYGPVGSTTFETARENFLISSAGYAVASLLLQPKDRHNGNLLFDDVGRLVHIDF 1920

Query: 1921 GFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSETWNLFVRLCVKGYLAARRHMHG 1980
            GFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKS+TW+ FV LCVKGYLAARR M G
Sbjct: 1921 GFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSKTWHQFVSLCVKGYLAARRQMDG 1980

Query: 1981 IINTVLLMLDSGLPCFSRGDPIGNLRKRFHPEMNDREAANFMIRVCTDAYNKWTTAGYDL 2035
            II+TV +ML+SGLPCFSRGDPIGNLRKRFHPEM++REAA+FMI VCTDAYNKWTTAGYDL
Sbjct: 1981 IISTVQMMLESGLPCFSRGDPIGNLRKRFHPEMSEREAAHFMIHVCTDAYNKWTTAGYDL 2028

BLAST of Chy1G008030 vs. TAIR 10
Match: AT1G51040.1 (Protein kinase superfamily protein )

HSP 1 Score: 696.8 bits (1797), Expect = 5.1e-200
Identity = 345/420 (82.14%), Postives = 373/420 (88.81%), Query Frame = 0

Query: 1615 LVEGYLLRAAKRSDIFAHILIWHLQGETSLPDSGKDVNSGKNGSFLALLPVVRQRIIDGF 1674
            +VEGYLLRAA+RSDIFAH LIWHLQGE S+ +  KD    KN SF  +L  VRQ I+DGF
Sbjct: 115  MVEGYLLRAAQRSDIFAHTLIWHLQGE-SVEEIVKDGAFDKNASFQEILSDVRQHIVDGF 174

Query: 1675 TPKALDLFKREFDFFDKVTSISGVLFPLPKDERRAGIRSELEKIEMEGEDLYLPTATNKL 1734
            TPKAL+ F REFDFF+KVTSISG L PLPK+ER AGIR ELEKI+M+GEDLYLPTA NKL
Sbjct: 175  TPKALNFFSREFDFFEKVTSISGALLPLPKEERVAGIRRELEKIKMQGEDLYLPTAPNKL 234

Query: 1735 VCGIQVDSGIPLQSAAKVPIMVTFNVVDRDGDPNNIKPQACIFKVGDDCRQDVLALQVIS 1794
            V  IQVDSGIPLQSAAKVPIM+TFNVVD DGD N++KPQACIFKVGDDCRQDVLALQVIS
Sbjct: 235  VRVIQVDSGIPLQSAAKVPIMITFNVVDLDGDHNDVKPQACIFKVGDDCRQDVLALQVIS 294

Query: 1795 LLRDIFQAVGLNLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGP 1854
            LL DIFQAVGLNLYLFPYGVLPT        VVPNTRSRSQMGETTDGGLYEIFQQ+YG 
Sbjct: 295  LLGDIFQAVGLNLYLFPYGVLPT--------VVPNTRSRSQMGETTDGGLYEIFQQNYGL 354

Query: 1855 VGSPNFEAARENFIVSSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFILETSPGG 1914
            VGS  FE AR NF++SSAGYAVASLLLQPKDRHNGNLLFD+VGRLVHIDFGFILETSPGG
Sbjct: 355  VGSTTFETARANFLISSAGYAVASLLLQPKDRHNGNLLFDDVGRLVHIDFGFILETSPGG 414

Query: 1915 NMRFESAHFKLSHEMTQLLDPSGVMKSETWNLFVRLCVKGYLAARRHMHGIINTVLLMLD 1974
            NMRFE+AHFKLSHEMTQLLDPSGVMKS+TW+ FV LCVKGYLAARR+M  II+TV +ML+
Sbjct: 415  NMRFENAHFKLSHEMTQLLDPSGVMKSKTWHQFVSLCVKGYLAARRYMDEIISTVQMMLE 474

Query: 1975 SGLPCFSRGDPIGNLRKRFHPEMNDREAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK 2034
            SGLPCFSRGDPIGNLRKRFHPEM++REAA FMI VCTDAYNKWTTAGYDLIQYLQQG+EK
Sbjct: 475  SGLPCFSRGDPIGNLRKRFHPEMSEREAALFMINVCTDAYNKWTTAGYDLIQYLQQGVEK 525

HSP 2 Score: 112.5 bits (280), Expect = 4.1e-24
Identity = 55/109 (50.46%), Postives = 74/109 (67.89%), Query Frame = 0

Query: 1   MDALIELCDLIAQNPALFSEKLSWICSRCPPSEAILAGSPAISRSQLNAVLAVARLLSKC 60
           M+ L ELCD+I QNP  F +KL WIC +CP S+ + A SP  S+S LNA+LAV R+LSK 
Sbjct: 1   MERLNELCDIIEQNPKQFLDKLPWICQQCPQSKLLRAESPRFSQSHLNAILAVTRILSKI 60

Query: 61  PDSVGLRPKSVVLEFLRSIPLSFSLSFWPQSYGNDAIASFFNEFLNYTS 110
            D+     K VVL+FL+++P SF  SFWP S   ++I++F+  FL Y S
Sbjct: 61  VDTTDENAKFVVLDFLQTVPKSFHRSFWPYSLSLESISAFYCSFLGYVS 109

BLAST of Chy1G008030 vs. TAIR 10
Match: AT5G64070.1 (phosphatidylinositol 4-OH kinase beta1 )

HSP 1 Score: 210.7 bits (535), Expect = 1.1e-53
Identity = 114/266 (42.86%), Postives = 162/266 (60.90%), Query Frame = 0

Query: 1773 QACIFKVGDDCRQDVLALQVISLLRDIFQAVGLNLYLFPYGVLPTGPGRGIIEVVPNTRS 1832
            ++ I K GDDCRQ+ LA+Q+IS   DIFQ  GL L+L PY VL T     +IE +P+T S
Sbjct: 859  RSIIVKSGDDCRQEHLAVQLISHFYDIFQEAGLPLWLRPYEVLVTSSYTALIETIPDTAS 918

Query: 1833 ----RSQMGETTDGGLYEIFQQDYGPVGSPNFEAARENFIVSSAGYAVASLLLQPKDRHN 1892
                +S+    T   L + F   Y    SP+F+ A+ NF+ S AGY++   LLQ KDRHN
Sbjct: 919  IHSIKSRYPNIT--SLRDFFVAKY-KENSPSFKLAQRNFVESMAGYSLVCYLLQVKDRHN 978

Query: 1893 GNLLFDNVGRLVHIDFGFILETSPGGNMRFESAHFKLSHEMTQLLD-PSGVMKSETWNLF 1952
            GNLL D  G ++HIDFGF+L  SPGG + FESA FKL+ E+ +++D  +  + SE ++ F
Sbjct: 979  GNLLLDEEGHIIHIDFGFMLSNSPGG-VNFESAPFKLTRELLEVMDSDADGVPSEFFDYF 1038

Query: 1953 VRLCVKGYLAARRHMHGIINTVLLMLDSGLPCFSRGD-PIGNLRKRFHPEMNDREAANFM 2012
              LC++G+L  R+H   II  V ++ DSG PCF  G   I NLRKRFH  + + +  + +
Sbjct: 1039 KVLCIQGFLTCRKHAERIILLVEMLQDSGFPCFKGGPRTIQNLRKRFHLSLTEEQCVSLV 1098

Query: 2013 IRVCTDAYNKWTTAGYDLIQYLQQGI 2033
            + + + + + W T  YD  Q +  GI
Sbjct: 1099 LSLISSSLDAWRTRQYDYYQRVLNGI 1120

BLAST of Chy1G008030 vs. TAIR 10
Match: AT5G09350.1 (phosphatidylinositol 4-OH kinase beta2 )

HSP 1 Score: 209.9 bits (533), Expect = 1.9e-53
Identity = 113/266 (42.48%), Postives = 161/266 (60.53%), Query Frame = 0

Query: 1773 QACIFKVGDDCRQDVLALQVISLLRDIFQAVGLNLYLFPYGVLPTGPGRGIIEVVPNTRS 1832
            ++ I K GDDCRQ+ LA+Q+IS   DIFQ  GL L+L PY VL T     +IE +P+T S
Sbjct: 854  RSIIVKSGDDCRQEHLAVQLISHFFDIFQEAGLPLWLRPYEVLVTSSYTALIETIPDTAS 913

Query: 1833 ----RSQMGETTDGGLYEIFQQDYGPVGSPNFEAARENFIVSSAGYAVASLLLQPKDRHN 1892
                +S+    T   L + F   +    SP+F+ A+ NF+ S AGY++   LLQ KDRHN
Sbjct: 914  IHSIKSRYPNIT--SLRDFFDAKF-KENSPSFKLAQRNFVESMAGYSLVCYLLQIKDRHN 973

Query: 1893 GNLLFDNVGRLVHIDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGV-MKSETWNLF 1952
            GNLL D  G ++HIDFGF+L  SPGG + FESA FKL+ E+ +++D     + SE ++ F
Sbjct: 974  GNLLMDEEGHIIHIDFGFMLSNSPGG-VNFESAPFKLTRELLEVMDSDAEGLPSEFFDYF 1033

Query: 1953 VRLCVKGYLAARRHMHGIINTVLLMLDSGLPCFSRGD-PIGNLRKRFHPEMNDREAANFM 2012
              LC++G+L  R+H   II  V ++ DSG PCF  G   I NLRKRFH  + + +  + +
Sbjct: 1034 KVLCIQGFLTCRKHAERIILLVEMLQDSGFPCFKGGPRTIQNLRKRFHLSLTEEQCVSLV 1093

Query: 2013 IRVCTDAYNKWTTAGYDLIQYLQQGI 2033
            + + + + + W T  YD  Q +  GI
Sbjct: 1094 LSLISSSLDAWRTRQYDYYQRVLNGI 1115