Chy1G003220 (gene) Cucumber (hystrix) v1

Overview


Name	Chy1G003220
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	E3 ubiquitin-protein ligase EL5
Location	chrH01: 2322658 .. 2324343 (+)
RNA-Seq Expression	Chy1G003220
Synteny	Chy1G003220

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGCAGGCGTTCTACTAATTTTCCTCCAGGAATTTGGCAGGCCAGAAGGGAACACGATCGCCAGAGATATAACAGAAATGGATCAAACCGAACGAACTCCACACCTTCACACACTCAATCATTTCAGGTCAGCGGGTTAACGATTCTTTTGTTTTGCAACGAACTGTATTCCAATCCCACCATCAAAGCTTATGCTTTTATCCTACGACATGATTTGCAGGCCATAAAACAGAACTCGTAGATCTAGATCACATTTAAATTCCCTGTTGATAGTTCTCCTAGTTTTCATATATTAGTACTTGAGAGGTTAAAGAGATGATAACAATTGTATAGCTCATGCTCATGCTCGTTCTCGTTCATGTGTTGTCAGAATTCTTACAGAGAGATAGATCCAGTTCCATGGAGACCTGCTGGGGAGACAGTGATAACAAGAATATTGGACATCGGCAGGTAAATAACACAAGAGCTTAATTAGTAGTTATATTGGAACTTTTGGTGTTTTGGATTGAATTATATGCAAACCAAAGAAGTTGGTATTGCAGATTCACTTCGCAAAATGATTTGAAATTATGAAATCTTAGAGGCCAGGCAAAGTTTGCTGTATATTGCCTGGAAATAACATGGCCTAACAGCTAACTCAGGAAGATAACTTGGCAGAGAAGCAAGAAGTCCATGACTTTCACAAGTGGAAGAATTTGGGGGAAACAAATCACAAAGTGTCAGAACTTGGAGACTGATTTGTTGGAGATTCTCTGACTTAAGGGAGTTCAACAACATTCAACATCACACAATGCATTAAGCACAAATTTGTTCAACAATAATTCATATCACAATTTGCAGGGAAATAGAAATATGGCATTCTTGATTGAATTGCTTATTATAAATAGCTATAACTTTTGAGAAGAACAGATAGCAATAACTTTCAATTTTTTTCATGAGCAGCATCCAAATTTGCAATAACAAACTAAATCCCCTTTGACAATATCATTTGGTTTCAGTTTCAGAGGGAATCTACTTACATTGCCTTACATCCTGCAAGTAAATTCCATATCCTCCATACTTCGTTGATAACTCATTGACATTTTGAGAAATTTCCAGCCGTCCAGAAGAACCGCCTGCAATAGTCAGAATCATCACACACTCAAGACCAAGACAGCTTGAGGCCAGTCCAATACCTGCGGAGCTATACTCGCCAACTCCAAGATCCGAAGGGAACTCGACGCTAACATCTGAAGAGCAAAATATGGCACTGAATAAGCTGAAGAAAAAAGTGTATGATCCTCCGAGTAACACAAGTAGGAGAATAAGTTTCTATTACAGAGAGAATAACAGAGTAAACTCCAATGAGAGGCACAACAGCAATAGTGATGAAGAAGGCAAGGGATGCGCCGTTTGCCTTGAAGATTTCCAGCCAAAAGAGGAGGTGATGACGACGCCGTGCAACCATATGTTTCACGAACAATGCATAGTTCCGTGGGTGAAGAGCCACGGCCAATGTCCGGTTTGCAGATTTACCTTGTTCGAACGAACAGAGCGAAGAGGATCAGCTTCTTCCAATTTCAACAACAACAGTGTATCAATTGCGCCGCCTAGTGGCCTAGTGGACGACGAGCTCGTGTACTTGATACGAGCCATGGAAGAGGCCCTGATGCTACGAAATGTGGCGGAATTTTGGGGAGCATGA

mRNA sequence

ATGAGCAGGCGTTCTACTAATTTTCCTCCAGGAATTTGGCAGGCCAGAAGGGAACACGATCGCCAGAGATATAACAGAAATGGATCAAACCGAACGAACTCCACACCTTCACACACTCAATCATTTCAGAATTCTTACAGAGAGATAGATCCAGTTCCATGGAGACCTGCTGGGGAGACAGTGATAACAAGAATATTGGACATCGGCAGCCGTCCAGAAGAACCGCCTGCAATAGTCAGAATCATCACACACTCAAGACCAAGACAGCTTGAGGCCAGTCCAATACCTGCGGAGCTATACTCGCCAACTCCAAGATCCGAAGGGAACTCGACGCTAACATCTGAAGAGCAAAATATGGCACTGAATAAGCTGAAGAAAAAAGTGTATGATCCTCCGAGTAACACAAGTAGGAGAATAAGTTTCTATTACAGAGAGAATAACAGAGTAAACTCCAATGAGAGGCACAACAGCAATAGTGATGAAGAAGGCAAGGGATGCGCCGTTTGCCTTGAAGATTTCCAGCCAAAAGAGGAGGTGATGACGACGCCGTGCAACCATATGTTTCACGAACAATGCATAGTTCCGTGGGTGAAGAGCCACGGCCAATGTCCGGTTTGCAGATTTACCTTGTTCGAACGAACAGAGCGAAGAGGATCAGCTTCTTCCAATTTCAACAACAACAGTGTATCAATTGCGCCGCCTAGTGGCCTAGTGGACGACGAGCTCGTGTACTTGATACGAGCCATGGAAGAGGCCCTGATGCTACGAAATGTGGCGGAATTTTGGGGAGCATGA

Coding sequence (CDS)

ATGAGCAGGCGTTCTACTAATTTTCCTCCAGGAATTTGGCAGGCCAGAAGGGAACACGATCGCCAGAGATATAACAGAAATGGATCAAACCGAACGAACTCCACACCTTCACACACTCAATCATTTCAGAATTCTTACAGAGAGATAGATCCAGTTCCATGGAGACCTGCTGGGGAGACAGTGATAACAAGAATATTGGACATCGGCAGCCGTCCAGAAGAACCGCCTGCAATAGTCAGAATCATCACACACTCAAGACCAAGACAGCTTGAGGCCAGTCCAATACCTGCGGAGCTATACTCGCCAACTCCAAGATCCGAAGGGAACTCGACGCTAACATCTGAAGAGCAAAATATGGCACTGAATAAGCTGAAGAAAAAAGTGTATGATCCTCCGAGTAACACAAGTAGGAGAATAAGTTTCTATTACAGAGAGAATAACAGAGTAAACTCCAATGAGAGGCACAACAGCAATAGTGATGAAGAAGGCAAGGGATGCGCCGTTTGCCTTGAAGATTTCCAGCCAAAAGAGGAGGTGATGACGACGCCGTGCAACCATATGTTTCACGAACAATGCATAGTTCCGTGGGTGAAGAGCCACGGCCAATGTCCGGTTTGCAGATTTACCTTGTTCGAACGAACAGAGCGAAGAGGATCAGCTTCTTCCAATTTCAACAACAACAGTGTATCAATTGCGCCGCCTAGTGGCCTAGTGGACGACGAGCTCGTGTACTTGATACGAGCCATGGAAGAGGCCCTGATGCTACGAAATGTGGCGGAATTTTGGGGAGCATGA

Protein sequence

MSRRSTNFPPGIWQARREHDRQRYNRNGSNRTNSTPSHTQSFQNSYREIDPVPWRPAGETVITRILDIGSRPEEPPAIVRIITHSRPRQLEASPIPAELYSPTPRSEGNSTLTSEEQNMALNKLKKKVYDPPSNTSRRISFYYRENNRVNSNERHNSNSDEEGKGCAVCLEDFQPKEEVMTTPCNHMFHEQCIVPWVKSHGQCPVCRFTLFERTERRGSASSNFNNNSVSIAPPSGLVDDELVYLIRAMEEALMLRNVAEFWGA*

Homology

[Close]

BLAST of Chy1G003220 vs. ExPASy Swiss-Prot
Match: Q9D0C1 (E3 ubiquitin-protein ligase RNF115 OS=Mus musculus OX=10090 GN=Rnf115 PE=1 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 8.6e-12
Identity = 33/72 (45.83%), Postives = 42/72 (58.33%), Query Frame = 0


Query: 163 GKGCAVCLEDFQPKEEVMTTPCNHMFHEQCIVPWVKSHGQCPVCRFTLFERTERR----- 222
           G  C VC ED+  +E+V   PCNH FH  CIVPW++ H  CPVCR +L      R     
Sbjct: 226 GLECPVCKEDYTVEEKVRQLPCNHFFHSSCIVPWLELHDTCPVCRKSLNGEDSTRQTQSS 285

Query: 223 -GSASSNFNNNS 229
             SAS+ F+N+S
Sbjct: 286 EASASNRFSNDS 297

[Close]

BLAST of Chy1G003220 vs. ExPASy Swiss-Prot
Match: P0CH30 (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 3.3e-11
Identity = 31/80 (38.75%), Postives = 47/80 (58.75%), Query Frame = 0


Query: 157 SNSDEEGKGCAVCLEDFQPKEEVMTTPCNHMFHEQCIVPWVKSHGQCPVCRFTL------ 216
           SN + E   CAVC++DF+   E    PC H++H+ C++PW++ H  CPVCR  L      
Sbjct: 215 SNLNSEFNQCAVCMDDFEEGTEAKQMPCKHLYHKDCLLPWLELHNSCPVCRHELPTDDPD 274

Query: 217 FERTER--RGSASSNFNNNS 229
           +ER  R  +G++  N  +NS
Sbjct: 275 YERRVRGAQGTSGGNDGDNS 294

[Close]

BLAST of Chy1G003220 vs. ExPASy Swiss-Prot
Match: Q6IRP0 (E3 ubiquitin-protein ligase RNF126-B OS=Xenopus laevis OX=8355 GN=rnf126-b PE=2 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 4.3e-11
Identity = 31/81 (38.27%), Postives = 44/81 (54.32%), Query Frame = 0


Query: 163 GKGCAVCLEDFQPKEEVMTTPCNHMFHEQCIVPWVKSHGQCPVCRFTLFER---TERRGS 222
           G  C VC ED+   E V   PCNH+FH  CI+PW++ H  CPVCR +L  +   T   G 
Sbjct: 225 GLECPVCKEDYTVGESVRQLPCNHLFHNDCIIPWLEQHDTCPVCRKSLSGQNTATNPPGL 284

Query: 223 ASSNFNNNSVSIAPPSGLVDD 241
               F+++S S +  +   D+
Sbjct: 285 TDMTFSSSSTSSSSSTSPTDE 305

[Close]

BLAST of Chy1G003220 vs. ExPASy Swiss-Prot
Match: Q940T5 (E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 4.3e-11
Identity = 29/66 (43.94%), Postives = 40/66 (60.61%), Query Frame = 0


Query: 166 CAVCLEDFQPKEEVMTTPCNHMFHEQCIVPWVKSHGQCPVCRFTLFERTERRGSASSNFN 225
           CAVC E F+ + E    PC H+FH+ CIVPW+     CPVCRF L     RR  +++N  
Sbjct: 199 CAVCTEIFETETEAREMPCKHLFHDDCIVPWLSIRNSCPVCRFELPSEPNRR--SNNNEE 258

Query: 226 NNSVSI 232
           +N+V +
Sbjct: 259 DNAVGM 262

[Close]

BLAST of Chy1G003220 vs. ExPASy Swiss-Prot
Match: Q0II22 (E3 ubiquitin-protein ligase RNF126 OS=Bos taurus OX=9913 GN=RNF126 PE=2 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 4.3e-11
Identity = 32/77 (41.56%), Postives = 46/77 (59.74%), Query Frame = 0


Query: 163 GKGCAVCLEDFQPKEEVMTTPCNHMFHEQCIVPWVKSHGQCPVCRFTLFER---TERRGS 222
           G  C VC +D+   E V   PCNH+FH+ CIVPW++ H  CPVCR +L  +   T+  G 
Sbjct: 229 GLECPVCKDDYGLGEHVRQLPCNHLFHDGCIVPWLEQHDSCPVCRKSLTGQNTATDPPGL 288

Query: 223 ASSNFNNNSVSIAPPSG 237
           A  +F+++S S +   G
Sbjct: 289 AGVSFSSSSSSSSSSPG 305

[Close]

BLAST of Chy1G003220 vs. ExPASy TrEMBL
Match: A0A0A0K1V1 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G049220 PE=4 SV=1)

HSP 1 Score: 519.6 bits (1337), Expect = 7.6e-144
Identity = 257/264 (97.35%), Postives = 259/264 (98.11%), Query Frame = 0


Query: 1   MSRRSTNFPPGIWQARREHDRQRYNRNGSNRTNSTPSHTQSFQNSYREIDPVPWRPAGET 60
           MSRRSTNFPPGIWQARREHDRQRYNRN SNRTNSTPSHTQSFQNSYR+IDPVPWRPAGET
Sbjct: 1   MSRRSTNFPPGIWQARREHDRQRYNRNESNRTNSTPSHTQSFQNSYRQIDPVPWRPAGET 60

Query: 61  VITRILDIGSRPEEPPAIVRIITHSRPRQLEASPIPAELYSPTPRSEGNSTLTSEEQNMA 120
           VITRILDIGSRPEEPPAIVRIITHSRPRQLEASPIPAELYSPTPRSEGNSTLTSEEQNMA
Sbjct: 61  VITRILDIGSRPEEPPAIVRIITHSRPRQLEASPIPAELYSPTPRSEGNSTLTSEEQNMA 120

Query: 121 LNKLKKKVYDPPSNTSRRISFYYRENNRVNSNERHNSNSDEEGKGCAVCLEDFQPKEEVM 180
           LNKLKKKVYDPPSNTSRRISFYYRENNRVNSNERHNSN DEEGK CAVCLEDFQPKEEVM
Sbjct: 121 LNKLKKKVYDPPSNTSRRISFYYRENNRVNSNERHNSNGDEEGKRCAVCLEDFQPKEEVM 180

Query: 181 TTPCNHMFHEQCIVPWVKSHGQCPVCRFTLFERTERRGSASSNFNNNSVSIAPPSGLVDD 240
           TTPCNHMFHEQCIVPWVKSHGQCPVCRFTLFERTERRGSASSNF NNSVSIAPPS LVD+
Sbjct: 181 TTPCNHMFHEQCIVPWVKSHGQCPVCRFTLFERTERRGSASSNF-NNSVSIAPPSSLVDE 240

Query: 241 ELVYLIRAMEEALMLRNVAEFWGA 265
           ELVYLIRAMEEALMLRNVAEFWGA
Sbjct: 241 ELVYLIRAMEEALMLRNVAEFWGA 263

[Close]

BLAST of Chy1G003220 vs. ExPASy TrEMBL
Match: A0A1S3C054 (E3 ubiquitin-protein ligase RNF12 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103495488 PE=4 SV=1)

HSP 1 Score: 499.6 bits (1285), Expect = 8.1e-138
Identity = 250/265 (94.34%), Postives = 257/265 (96.98%), Query Frame = 0


Query: 1   MSRRSTNFPPGIWQARREHDRQRYNRNGSNRTNSTPSHTQSFQNSYREIDPVPWRPAGET 60
           MSRRSTNFPPGIWQARRE+DRQRYNRN SNR NSTPSHTQSFQNSYREIDPVPWRPAGET
Sbjct: 1   MSRRSTNFPPGIWQARREYDRQRYNRNESNRVNSTPSHTQSFQNSYREIDPVPWRPAGET 60

Query: 61  VITRILDIGSRPEEPPAIVRIITHSRPRQLEASPIPAELYSPTPRSEGNSTLTSEEQNMA 120
           +ITRILDIGS PEEPPAIVRIITHSRPRQ+EASPIPAELYSPTPRSEGNSTLTSEEQNMA
Sbjct: 61  MITRILDIGSLPEEPPAIVRIITHSRPRQIEASPIPAELYSPTPRSEGNSTLTSEEQNMA 120

Query: 121 LNKLKKKVYDPPSNTSRRISFYYRENNRVNSNERHNSNSDEEGKGCAVCLEDFQPKEEVM 180
           LNKLKKKVYDPPSNTSRRI+FYYRENNRVN NERHN+NSDEEGKGCAVCLEDFQPKEEVM
Sbjct: 121 LNKLKKKVYDPPSNTSRRINFYYRENNRVNYNERHNNNSDEEGKGCAVCLEDFQPKEEVM 180

Query: 181 TTPCNHMFHEQCIVPWVKSHGQCPVCRFTLFE-RTERRGSASSNFNNNSVSIAPPSGLVD 240
           TTPCNHMFHEQCIVPWVKS+GQCPVCRFTLFE RTERRG ASSNFNNN VSIAPPS LVD
Sbjct: 181 TTPCNHMFHEQCIVPWVKSNGQCPVCRFTLFERRTERRG-ASSNFNNN-VSIAPPSSLVD 240

Query: 241 DELVYLIRAMEEALMLRNVAEFWGA 265
           DEL+YLIRAMEEALMLRNVAEFWGA
Sbjct: 241 DELMYLIRAMEEALMLRNVAEFWGA 263

[Close]

BLAST of Chy1G003220 vs. ExPASy TrEMBL
Match: A0A5D3C526 (E3 ubiquitin-protein ligase RNF12 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G003170 PE=4 SV=1)

HSP 1 Score: 494.6 bits (1272), Expect = 2.6e-136
Identity = 248/263 (94.30%), Postives = 255/263 (96.96%), Query Frame = 0


Query: 1   MSRRSTNFPPGIWQARREHDRQRYNRNGSNRTNSTPSHTQSFQNSYREIDPVPWRPAGET 60
           MSRRSTNFPPGIWQARRE+DRQRYNRN SNR NSTPSHTQSFQNSYREIDPVPWRPAGET
Sbjct: 1   MSRRSTNFPPGIWQARREYDRQRYNRNESNRVNSTPSHTQSFQNSYREIDPVPWRPAGET 60

Query: 61  VITRILDIGSRPEEPPAIVRIITHSRPRQLEASPIPAELYSPTPRSEGNSTLTSEEQNMA 120
           +ITRILDIGS PEEPPAIVRIITHSRPRQ+EASPIPAELYSPTPRSEGNSTLTSEEQNMA
Sbjct: 61  MITRILDIGSLPEEPPAIVRIITHSRPRQIEASPIPAELYSPTPRSEGNSTLTSEEQNMA 120

Query: 121 LNKLKKKVYDPPSNTSRRISFYYRENNRVNSNERHNSNSDEEGKGCAVCLEDFQPKEEVM 180
           LNKLKKKVYDPPSNTSRRI+FYYRENNRVN NERHN+NSDEEGKGCAVCLEDFQPKEEVM
Sbjct: 121 LNKLKKKVYDPPSNTSRRINFYYRENNRVNYNERHNNNSDEEGKGCAVCLEDFQPKEEVM 180

Query: 181 TTPCNHMFHEQCIVPWVKSHGQCPVCRFTLFE-RTERRGSASSNFNNNSVSIAPPSGLVD 240
           TTPCNHMFHEQCIVPWVKS+GQCPVCRFTLFE RTERRG ASSNF NNSVSIAPPS LVD
Sbjct: 181 TTPCNHMFHEQCIVPWVKSNGQCPVCRFTLFERRTERRG-ASSNF-NNSVSIAPPSSLVD 240

Query: 241 DELVYLIRAMEEALMLRNVAEFW 263
           DEL+YLIRAMEEALMLRNVAEFW
Sbjct: 241 DELMYLIRAMEEALMLRNVAEFW 261

[Close]

BLAST of Chy1G003220 vs. ExPASy TrEMBL
Match: A0A5A7SLQ9 (E3 ubiquitin-protein ligase RNF12 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold139G003130 PE=4 SV=1)

HSP 1 Score: 491.5 bits (1264), Expect = 2.2e-135
Identity = 247/263 (93.92%), Postives = 254/263 (96.58%), Query Frame = 0


Query: 1   MSRRSTNFPPGIWQARREHDRQRYNRNGSNRTNSTPSHTQSFQNSYREIDPVPWRPAGET 60
           MSRRSTNFPPGIWQARRE+DRQRYNRN SNR NSTPSHTQSFQNSYREIDPVPWRPAGET
Sbjct: 1   MSRRSTNFPPGIWQARREYDRQRYNRNESNRVNSTPSHTQSFQNSYREIDPVPWRPAGET 60

Query: 61  VITRILDIGSRPEEPPAIVRIITHSRPRQLEASPIPAELYSPTPRSEGNSTLTSEEQNMA 120
           +ITRILDIGS PEEPPAIVRIITHSRPRQ+EASPIPAELYSPTPRSEGNSTLTSEEQNMA
Sbjct: 61  MITRILDIGSLPEEPPAIVRIITHSRPRQIEASPIPAELYSPTPRSEGNSTLTSEEQNMA 120

Query: 121 LNKLKKKVYDPPSNTSRRISFYYRENNRVNSNERHNSNSDEEGKGCAVCLEDFQPKEEVM 180
           LNKLKKKVYDPPSNTSRRI+FYYRENNRVN NERHN+NSDEEGKGCAVCLEDFQ KEEVM
Sbjct: 121 LNKLKKKVYDPPSNTSRRINFYYRENNRVNYNERHNNNSDEEGKGCAVCLEDFQSKEEVM 180

Query: 181 TTPCNHMFHEQCIVPWVKSHGQCPVCRFTLFE-RTERRGSASSNFNNNSVSIAPPSGLVD 240
           TTPCNHMFHEQCIVPWVKS+GQCPVCRFTLFE RTERRG ASSNF NNSVSIAPPS LVD
Sbjct: 181 TTPCNHMFHEQCIVPWVKSNGQCPVCRFTLFERRTERRG-ASSNF-NNSVSIAPPSSLVD 240

Query: 241 DELVYLIRAMEEALMLRNVAEFW 263
           DEL+YLIRAMEEALMLRNVAEFW
Sbjct: 241 DELMYLIRAMEEALMLRNVAEFW 261

[Close]

BLAST of Chy1G003220 vs. ExPASy TrEMBL
Match: A0A6J1JPC8 (E3 ubiquitin-protein ligase EL5 OS=Cucurbita maxima OX=3661 GN=LOC111486293 PE=4 SV=1)

HSP 1 Score: 397.5 bits (1020), Expect = 4.3e-107
Identity = 202/285 (70.88%), Postives = 224/285 (78.60%), Query Frame = 0


Query: 1   MSRRSTNFPPGIWQARREHDRQRYNRNGSNRTNSTPSHTQSFQNS--------------- 60
           MSRRSTNFPPG+W+ARRE+DRQRYN N SNR +ST  H Q FQNS               
Sbjct: 1   MSRRSTNFPPGLWRARREYDRQRYNTNDSNRMSSTSPHPQPFQNSSRDTEPIPSRPTRGI 60

Query: 61  -------YREIDPVPWRPAGETVITRILDIGSRPEEPPAIVRIITHSRPRQLEASPIPAE 120
                   RE+DP+PWRP G  +I R+L+I  + E+PPAIVRIITHSRPRQ+EAS  PAE
Sbjct: 61  DPVPSRPTRELDPIPWRPTGGAMIARLLEIVGQLEDPPAIVRIITHSRPRQIEASSRPAE 120

Query: 121 LYSPTPRSEGNSTLTSEEQNMALNKLKKKVYDPPSNTSRRISFYYRENNRVNSNERHNSN 180
           LY PTPRSEGNSTLTSEEQNMALNKLKKKVYDPP+N SRRISFYYRENNR N NER N  
Sbjct: 121 LYRPTPRSEGNSTLTSEEQNMALNKLKKKVYDPPNNASRRISFYYRENNRRNYNERRNGE 180

Query: 181 SDEEGKGCAVCLEDFQPKEEVMTTPCNHMFHEQCIVPWVKSHGQCPVCRFTLFERTERRG 240
           +DE+GK CAVCLEDFQPKEEVMTTPCNHMFHE+CIVPWVKSHGQCP CRFTLFERT RR 
Sbjct: 181 NDEDGKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKSHGQCPNCRFTLFERTVRRE 240

Query: 241 SASSNFNNNSVSIAPPSGLVDDELVYLIRAMEEALMLRNVAEFWG 264
           ++S    NN+ SIA  S LVDDELVYLIRAME++LMLRN+   WG
Sbjct: 241 ASS----NNTASIASGSNLVDDELVYLIRAMEDSLMLRNLTGLWG 281

[Close]

BLAST of Chy1G003220 vs. NCBI nr
Match: XP_004136838.1 (E3 ubiquitin-protein ligase AIRP1 [Cucumis sativus] >KGN43640.1 hypothetical protein Csa_017086 [Cucumis sativus])

HSP 1 Score: 520 bits (1339), Expect = 6.84e-186
Identity = 257/264 (97.35%), Postives = 259/264 (98.11%), Query Frame = 0


Query: 1   MSRRSTNFPPGIWQARREHDRQRYNRNGSNRTNSTPSHTQSFQNSYREIDPVPWRPAGET 60
           MSRRSTNFPPGIWQARREHDRQRYNRN SNRTNSTPSHTQSFQNSYR+IDPVPWRPAGET
Sbjct: 1   MSRRSTNFPPGIWQARREHDRQRYNRNESNRTNSTPSHTQSFQNSYRQIDPVPWRPAGET 60

Query: 61  VITRILDIGSRPEEPPAIVRIITHSRPRQLEASPIPAELYSPTPRSEGNSTLTSEEQNMA 120
           VITRILDIGSRPEEPPAIVRIITHSRPRQLEASPIPAELYSPTPRSEGNSTLTSEEQNMA
Sbjct: 61  VITRILDIGSRPEEPPAIVRIITHSRPRQLEASPIPAELYSPTPRSEGNSTLTSEEQNMA 120

Query: 121 LNKLKKKVYDPPSNTSRRISFYYRENNRVNSNERHNSNSDEEGKGCAVCLEDFQPKEEVM 180
           LNKLKKKVYDPPSNTSRRISFYYRENNRVNSNERHNSN DEEGK CAVCLEDFQPKEEVM
Sbjct: 121 LNKLKKKVYDPPSNTSRRISFYYRENNRVNSNERHNSNGDEEGKRCAVCLEDFQPKEEVM 180

Query: 181 TTPCNHMFHEQCIVPWVKSHGQCPVCRFTLFERTERRGSASSNFNNNSVSIAPPSGLVDD 240
           TTPCNHMFHEQCIVPWVKSHGQCPVCRFTLFERTERRGSASSNFNN SVSIAPPS LVD+
Sbjct: 181 TTPCNHMFHEQCIVPWVKSHGQCPVCRFTLFERTERRGSASSNFNN-SVSIAPPSSLVDE 240

Query: 241 ELVYLIRAMEEALMLRNVAEFWGA 264
           ELVYLIRAMEEALMLRNVAEFWGA
Sbjct: 241 ELVYLIRAMEEALMLRNVAEFWGA 263

[Close]

BLAST of Chy1G003220 vs. NCBI nr
Match: XP_008455285.1 (PREDICTED: E3 ubiquitin-protein ligase RNF12 isoform X1 [Cucumis melo])

HSP 1 Score: 500 bits (1287), Expect = 5.78e-178
Identity = 250/265 (94.34%), Postives = 257/265 (96.98%), Query Frame = 0


Query: 1   MSRRSTNFPPGIWQARREHDRQRYNRNGSNRTNSTPSHTQSFQNSYREIDPVPWRPAGET 60
           MSRRSTNFPPGIWQARRE+DRQRYNRN SNR NSTPSHTQSFQNSYREIDPVPWRPAGET
Sbjct: 1   MSRRSTNFPPGIWQARREYDRQRYNRNESNRVNSTPSHTQSFQNSYREIDPVPWRPAGET 60

Query: 61  VITRILDIGSRPEEPPAIVRIITHSRPRQLEASPIPAELYSPTPRSEGNSTLTSEEQNMA 120
           +ITRILDIGS PEEPPAIVRIITHSRPRQ+EASPIPAELYSPTPRSEGNSTLTSEEQNMA
Sbjct: 61  MITRILDIGSLPEEPPAIVRIITHSRPRQIEASPIPAELYSPTPRSEGNSTLTSEEQNMA 120

Query: 121 LNKLKKKVYDPPSNTSRRISFYYRENNRVNSNERHNSNSDEEGKGCAVCLEDFQPKEEVM 180
           LNKLKKKVYDPPSNTSRRI+FYYRENNRVN NERHN+NSDEEGKGCAVCLEDFQPKEEVM
Sbjct: 121 LNKLKKKVYDPPSNTSRRINFYYRENNRVNYNERHNNNSDEEGKGCAVCLEDFQPKEEVM 180

Query: 181 TTPCNHMFHEQCIVPWVKSHGQCPVCRFTLFER-TERRGSASSNFNNNSVSIAPPSGLVD 240
           TTPCNHMFHEQCIVPWVKS+GQCPVCRFTLFER TERRG ASSNFNNN VSIAPPS LVD
Sbjct: 181 TTPCNHMFHEQCIVPWVKSNGQCPVCRFTLFERRTERRG-ASSNFNNN-VSIAPPSSLVD 240

Query: 241 DELVYLIRAMEEALMLRNVAEFWGA 264
           DEL+YLIRAMEEALMLRNVAEFWGA
Sbjct: 241 DELMYLIRAMEEALMLRNVAEFWGA 263

[Close]

BLAST of Chy1G003220 vs. NCBI nr
Match: TYK07001.1 (E3 ubiquitin-protein ligase RNF12 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 495 bits (1275), Expect = 3.90e-176
Identity = 249/265 (93.96%), Postives = 256/265 (96.60%), Query Frame = 0


Query: 1   MSRRSTNFPPGIWQARREHDRQRYNRNGSNRTNSTPSHTQSFQNSYREIDPVPWRPAGET 60
           MSRRSTNFPPGIWQARRE+DRQRYNRN SNR NSTPSHTQSFQNSYREIDPVPWRPAGET
Sbjct: 1   MSRRSTNFPPGIWQARREYDRQRYNRNESNRVNSTPSHTQSFQNSYREIDPVPWRPAGET 60

Query: 61  VITRILDIGSRPEEPPAIVRIITHSRPRQLEASPIPAELYSPTPRSEGNSTLTSEEQNMA 120
           +ITRILDIGS PEEPPAIVRIITHSRPRQ+EASPIPAELYSPTPRSEGNSTLTSEEQNMA
Sbjct: 61  MITRILDIGSLPEEPPAIVRIITHSRPRQIEASPIPAELYSPTPRSEGNSTLTSEEQNMA 120

Query: 121 LNKLKKKVYDPPSNTSRRISFYYRENNRVNSNERHNSNSDEEGKGCAVCLEDFQPKEEVM 180
           LNKLKKKVYDPPSNTSRRI+FYYRENNRVN NERHN+NSDEEGKGCAVCLEDFQPKEEVM
Sbjct: 121 LNKLKKKVYDPPSNTSRRINFYYRENNRVNYNERHNNNSDEEGKGCAVCLEDFQPKEEVM 180

Query: 181 TTPCNHMFHEQCIVPWVKSHGQCPVCRFTLFER-TERRGSASSNFNNNSVSIAPPSGLVD 240
           TTPCNHMFHEQCIVPWVKS+GQCPVCRFTLFER TERRG ASSNFNN SVSIAPPS LVD
Sbjct: 181 TTPCNHMFHEQCIVPWVKSNGQCPVCRFTLFERRTERRG-ASSNFNN-SVSIAPPSSLVD 240

Query: 241 DELVYLIRAMEEALMLRNVAEFWGA 264
           DEL+YLIRAMEEALMLRNVAEFW A
Sbjct: 241 DELMYLIRAMEEALMLRNVAEFWIA 263

[Close]

BLAST of Chy1G003220 vs. NCBI nr
Match: KAA0031548.1 (E3 ubiquitin-protein ligase RNF12 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 492 bits (1267), Expect = 6.47e-175
Identity = 248/265 (93.58%), Postives = 255/265 (96.23%), Query Frame = 0


Query: 1   MSRRSTNFPPGIWQARREHDRQRYNRNGSNRTNSTPSHTQSFQNSYREIDPVPWRPAGET 60
           MSRRSTNFPPGIWQARRE+DRQRYNRN SNR NSTPSHTQSFQNSYREIDPVPWRPAGET
Sbjct: 1   MSRRSTNFPPGIWQARREYDRQRYNRNESNRVNSTPSHTQSFQNSYREIDPVPWRPAGET 60

Query: 61  VITRILDIGSRPEEPPAIVRIITHSRPRQLEASPIPAELYSPTPRSEGNSTLTSEEQNMA 120
           +ITRILDIGS PEEPPAIVRIITHSRPRQ+EASPIPAELYSPTPRSEGNSTLTSEEQNMA
Sbjct: 61  MITRILDIGSLPEEPPAIVRIITHSRPRQIEASPIPAELYSPTPRSEGNSTLTSEEQNMA 120

Query: 121 LNKLKKKVYDPPSNTSRRISFYYRENNRVNSNERHNSNSDEEGKGCAVCLEDFQPKEEVM 180
           LNKLKKKVYDPPSNTSRRI+FYYRENNRVN NERHN+NSDEEGKGCAVCLEDFQ KEEVM
Sbjct: 121 LNKLKKKVYDPPSNTSRRINFYYRENNRVNYNERHNNNSDEEGKGCAVCLEDFQSKEEVM 180

Query: 181 TTPCNHMFHEQCIVPWVKSHGQCPVCRFTLFER-TERRGSASSNFNNNSVSIAPPSGLVD 240
           TTPCNHMFHEQCIVPWVKS+GQCPVCRFTLFER TERRG ASSNFNN SVSIAPPS LVD
Sbjct: 181 TTPCNHMFHEQCIVPWVKSNGQCPVCRFTLFERRTERRG-ASSNFNN-SVSIAPPSSLVD 240

Query: 241 DELVYLIRAMEEALMLRNVAEFWGA 264
           DEL+YLIRAMEEALMLRNVAEFW A
Sbjct: 241 DELMYLIRAMEEALMLRNVAEFWIA 263

[Close]

BLAST of Chy1G003220 vs. NCBI nr
Match: XP_038888228.1 (uncharacterized protein LOC120078088 isoform X1 [Benincasa hispida])

HSP 1 Score: 468 bits (1205), Expect = 1.75e-165
Identity = 230/264 (87.12%), Postives = 246/264 (93.18%), Query Frame = 0


Query: 1   MSRRSTNFPPGIWQARREHDRQRYNRNGSNRTNSTPSHTQSFQNSYREIDPVPWRPAGET 60
           MSRRSTNFPPGIWQARRE+DRQRYNRN SNR NSTPSHT SFQNSYRE+DPVPWRPAGET
Sbjct: 1   MSRRSTNFPPGIWQARREYDRQRYNRNDSNRINSTPSHTNSFQNSYREVDPVPWRPAGET 60

Query: 61  VITRILDIGSRPEEPPAIVRIITHSRPRQLEASPIPAELYSPTPRSEGNSTLTSEEQNMA 120
           +I RILDIG   EEPPAIVRIIT SRPRQLEASPIPAELYSPTPRSEGNSTLTSEEQNMA
Sbjct: 61  MIARILDIGRPREEPPAIVRIITSSRPRQLEASPIPAELYSPTPRSEGNSTLTSEEQNMA 120

Query: 121 LNKLKKKVYDPPSNTSRRISFYYRENNRVNSNERHNSNSDEEGKGCAVCLEDFQPKEEVM 180
           L KLKK VYDPPSNTSRRISFYYRENNR N NER+NSNSDEEGK CAVCLEDFQPKEEVM
Sbjct: 121 LTKLKKNVYDPPSNTSRRISFYYRENNRGNYNERNNSNSDEEGKRCAVCLEDFQPKEEVM 180

Query: 181 TTPCNHMFHEQCIVPWVKSHGQCPVCRFTLFERTERRGSASSNFNNNSVSIAPPSGLVDD 240
           TTPCNHMFHE+CIVPWVKS+GQCPVCRFTLFERTERRG++S++  NN+VS APPS L+DD
Sbjct: 181 TTPCNHMFHEECIVPWVKSNGQCPVCRFTLFERTERRGASSNS--NNTVSTAPPSSLIDD 240

Query: 241 ELVYLIRAMEEALMLRNVAEFWGA 264
           +L+YLIRAME+ALMLRN+A FWGA
Sbjct: 241 DLMYLIRAMEDALMLRNLAGFWGA 262

[Close]

BLAST of Chy1G003220 vs. TAIR 10
Match: AT3G58720.1 (RING/U-box superfamily protein )

HSP 1 Score: 130.6 bits (327), Expect = 1.9e-30
Identity = 73/183 (39.89%), Postives = 104/183 (56.83%), Query Frame = 0


Query: 79  VRIITHSRPRQLEASPIPAELYSPTPRSEGNSTLTSEEQNMALNKLKKKVYD--PPSNTS 138
           +R   + R + LEA        S  P S   S+  + E++  L  L K+ Y+  P S   
Sbjct: 53  IRNSNNLRRQHLEALRQAVHNQSTRPESSSRSSSQTNEEDEILKHLTKETYNPVPKSTLL 112

Query: 139 RRISFYYRENNRVNSNERHNSN--SDEEGKGCAVCLEDFQPKEEVMTTPCNHMFHEQCIV 198
           R +S YYR  N  + + R+  N   +E+ K C+VCLEDF+PKE VM TPC HMFHE+CIV
Sbjct: 113 RSLSLYYRNKNPGSESSRNTQNLSGEEDDKRCSVCLEDFEPKETVMLTPCKHMFHEECIV 172

Query: 199 PWVKSHGQCPVCRFTLFERTERRGSASSNFNNNSVSIAPPSGLVDDELVYLIRAMEEALM 258
           PW+K+ GQCPVCRF + + T++  S  +N  +N       + L   EL+ ++RAMEE  +
Sbjct: 173 PWLKTKGQCPVCRFVILKPTKQDSSPPAN-GSNLTGDMTTNDLFTLELLSVVRAMEETFL 232

[Close]

BLAST of Chy1G003220 vs. TAIR 10
Match: AT3G58720.2 (RING/U-box superfamily protein )

HSP 1 Score: 130.6 bits (327), Expect = 1.9e-30
Identity = 73/183 (39.89%), Postives = 104/183 (56.83%), Query Frame = 0


Query: 79  VRIITHSRPRQLEASPIPAELYSPTPRSEGNSTLTSEEQNMALNKLKKKVYD--PPSNTS 138
           +R   + R + LEA        S  P S   S+  + E++  L  L K+ Y+  P S   
Sbjct: 81  IRNSNNLRRQHLEALRQAVHNQSTRPESSSRSSSQTNEEDEILKHLTKETYNPVPKSTLL 140

Query: 139 RRISFYYRENNRVNSNERHNSN--SDEEGKGCAVCLEDFQPKEEVMTTPCNHMFHEQCIV 198
           R +S YYR  N  + + R+  N   +E+ K C+VCLEDF+PKE VM TPC HMFHE+CIV
Sbjct: 141 RSLSLYYRNKNPGSESSRNTQNLSGEEDDKRCSVCLEDFEPKETVMLTPCKHMFHEECIV 200

Query: 199 PWVKSHGQCPVCRFTLFERTERRGSASSNFNNNSVSIAPPSGLVDDELVYLIRAMEEALM 258
           PW+K+ GQCPVCRF + + T++  S  +N  +N       + L   EL+ ++RAMEE  +
Sbjct: 201 PWLKTKGQCPVCRFVILKPTKQDSSPPAN-GSNLTGDMTTNDLFTLELLSVVRAMEETFL 260

[Close]

BLAST of Chy1G003220 vs. TAIR 10
Match: AT2G44330.1 (RING/U-box superfamily protein )

HSP 1 Score: 70.9 bits (172), Expect = 1.8e-12
Identity = 26/63 (41.27%), Postives = 40/63 (63.49%), Query Frame = 0


Query: 148 RVNSNERHNSNSDEEGKGCAVCLEDFQPKEEVMTTPCNHMFHEQCIVPWVKSHGQCPVCR 207
           +++S+   +++SD+    CA+C EDF   E     PCNH++H  CI+PW+ SH  CP+CR
Sbjct: 78  KISSSMLSSASSDDSALPCAICREDFVVGESARRLPCNHLYHNDCIIPWLTSHNSCPLCR 137

Query: 208 FTL 211
             L
Sbjct: 138 VEL 140

[Close]

BLAST of Chy1G003220 vs. TAIR 10
Match: AT1G55530.1 (RING/U-box superfamily protein )

HSP 1 Score: 70.5 bits (171), Expect = 2.3e-12
Identity = 29/72 (40.28%), Postives = 41/72 (56.94%), Query Frame = 0


Query: 161 EEGKGCAVCLEDFQPKEEVMTTPCNHMFHEQCIVPWVKSHGQCPVCRFTLFERTERRGSA 220
           EE   C+VCL+DF+   E    PC H FH  C++PW++ H  CPVCR+ L     +  S 
Sbjct: 218 EETLQCSVCLDDFEIGTEAKLMPCTHKFHSDCLLPWLELHSSCPVCRYQLPADEAKTDSV 277

Query: 221 SSNFNNNSVSIA 233
           ++  +NN  S A
Sbjct: 278 TTTSDNNGSSSA 289

[Close]

BLAST of Chy1G003220 vs. TAIR 10
Match: AT5G59550.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 70.1 bits (170), Expect = 3.0e-12
Identity = 29/66 (43.94%), Postives = 40/66 (60.61%), Query Frame = 0


Query: 166 CAVCLEDFQPKEEVMTTPCNHMFHEQCIVPWVKSHGQCPVCRFTLFERTERRGSASSNFN 225
           CAVC E F+ + E    PC H+FH+ CIVPW+     CPVCRF L     RR  +++N  
Sbjct: 199 CAVCTEIFETETEAREMPCKHLFHDDCIVPWLSIRNSCPVCRFELPSEPNRR--SNNNEE 258

Query: 226 NNSVSI 232
           +N+V +
Sbjct: 259 DNAVGM 262

The following BLAST results are available for this feature:

BLAST of Chy1G003220 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9D0C1	8.6e-12	45.83	E3 ubiquitin-protein ligase RNF115 OS=Mus musculus OX=10090 GN=Rnf115 PE=1 SV=1	[more]
P0CH30	3.3e-11	38.75	E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV...	[more]
Q6IRP0	4.3e-11	38.27	E3 ubiquitin-protein ligase RNF126-B OS=Xenopus laevis OX=8355 GN=rnf126-b PE=2 ...	[more]
Q940T5	4.3e-11	43.94	E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 ...	[more]
Q0II22	4.3e-11	41.56	E3 ubiquitin-protein ligase RNF126 OS=Bos taurus OX=9913 GN=RNF126 PE=2 SV=1	[more]

BLAST of Chy1G003220 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K1V1	7.6e-144	97.35	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G049220 P...	[more]
A0A1S3C054	8.1e-138	94.34	E3 ubiquitin-protein ligase RNF12 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10349...	[more]
A0A5D3C526	2.6e-136	94.30	E3 ubiquitin-protein ligase RNF12 isoform X1 OS=Cucumis melo var. makuwa OX=1194...	[more]
A0A5A7SLQ9	2.2e-135	93.92	E3 ubiquitin-protein ligase RNF12 isoform X1 OS=Cucumis melo var. makuwa OX=1194...	[more]
A0A6J1JPC8	4.3e-107	70.88	E3 ubiquitin-protein ligase EL5 OS=Cucurbita maxima OX=3661 GN=LOC111486293 PE=4...	[more]

BLAST of Chy1G003220 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004136838.1	6.84e-186	97.35	E3 ubiquitin-protein ligase AIRP1 [Cucumis sativus] >KGN43640.1 hypothetical pro...	[more]
XP_008455285.1	5.78e-178	94.34	PREDICTED: E3 ubiquitin-protein ligase RNF12 isoform X1 [Cucumis melo]	[more]
TYK07001.1	3.90e-176	93.96	E3 ubiquitin-protein ligase RNF12 isoform X1 [Cucumis melo var. makuwa]	[more]
KAA0031548.1	6.47e-175	93.58	E3 ubiquitin-protein ligase RNF12 isoform X1 [Cucumis melo var. makuwa]	[more]
XP_038888228.1	1.75e-165	87.12	uncharacterized protein LOC120078088 isoform X1 [Benincasa hispida]	[more]

BLAST of Chy1G003220 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G58720.1	1.9e-30	39.89	RING/U-box superfamily protein	[more]
AT3G58720.2	1.9e-30	39.89	RING/U-box superfamily protein	[more]
AT2G44330.1	1.8e-12	41.27	RING/U-box superfamily protein	[more]
AT1G55530.1	2.3e-12	40.28	RING/U-box superfamily protein	[more]
AT5G59550.1	3.0e-12	43.94	zinc finger (C3HC4-type RING finger) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 166..206 e-value: 5.2E-9 score: 45.9
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 166..207 e-value: 7.4E-14 score: 51.8
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 166..207 score: 13.374458
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 114..211 e-value: 4.7E-21 score: 76.3
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..51
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 27..47
None	No IPR available	PANTHER	PTHR22765:SF328	ANAPHASE-PROMOTING COMPLEX SUBUNIT 11 RING-H2 FINGER PROTEIN	coord: 117..258
None	No IPR available	PANTHER	PTHR22765	RING FINGER AND PROTEASE ASSOCIATED DOMAIN-CONTAINING	coord: 117..258
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 160..215

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy1G003220.1	Chy1G003220.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
biological_process	GO:0006511	ubiquitin-dependent protein catabolic process
molecular_function	GO:0061630	ubiquitin protein ligase activity