Chy1G002190 (gene) Cucumber (hystrix) v1

BLAST of Chy1G002190 vs. ExPASy Swiss-Prot
Match: Q0WKV8 (Probable ubiquitin-like-specific protease 2A OS=Arabidopsis thaliana OX=3702 GN=ULP2A PE=2 SV=2)

HSP 1 Score: 464.9 bits (1195), Expect = 1.8e-129
Identity = 243/549 (44.26%), Postives = 342/549 (62.30%), Query Frame = 0

Query: 26  KRFSVFDFSEEDARVEKVSRRLLGKFSA----RRSSPVTNHQFLHCFGKGAKSVSRNLSD 85
           K   VFD+S+ED RVE+ S++LL KF +    +    +  ++FL CF K  +S S+ L  
Sbjct: 13  KPIDVFDYSDEDDRVEEESKKLLRKFDSPVTKKHHCAIDKYEFLRCFAKDTQSESKVLQH 72

Query: 86  ELIDIDAKVGKSANIDSFSEDVSYELIHIDSEGHQISPGPTDLNAEDTCADTPLEGGGSM 145
            +ID++  V +  +    S D + +LI + S G     G   L +     +  +  G + 
Sbjct: 73  IVIDVEVPVKEEPSRCELSGDGNSDLIDVISNGSHRRIGIDSLTSSSLSENDEVSTGEA- 132

Query: 146 KQEILETNDLLLSRSSTNEDDVTVIFPDFVIYEGNWCTTSKLIFSCSCIKFRGSALSGLQ 205
                 TN          E+   +I PD +IY   +CT SKL FS +C+    S+++  +
Sbjct: 133 ------TNPASDPHEVDPENAQVLIIPDVIIYGDIYCTNSKLTFSRNCMNVESSSVNATK 192

Query: 206 RTFDSEWAISDIIGIESEWCSRVETAIVNLRLKGKHFTRAENSKDISGIELLKFSVCDPL 265
            TF  +W I DII IES+WC  VETA VN+ LK +     + +KDISGI+LLKFSV DP 
Sbjct: 193 GTFSCQWTIEDIIKIESQWCLEVETAFVNVLLKSRKPEGVDIAKDISGIDLLKFSVYDPK 252

Query: 266 WSESEKAIRTLNLRYNDLWNADYDDNDKVNGEGIVSWRHSDVFSPNNCFSEFVDTFEEVI 325
           WS+  + IR+L+ RY ++W     +++++   G                +   D+FE+++
Sbjct: 253 WSKEVETIRSLDSRYKNIWFDTITESEEIAFSG---------HDLGTSLTNLADSFEDLV 312

Query: 326 YPMGDPDAVTISKRDLELLKPGMFINDTIIDFYVKYLKNKFLLEKNNRFYFFNSFFFRKL 385
           YP G+PDAV + K+D+ELLKP  FINDTIIDFY+KYLKN+   ++  RF+FFN FFFRKL
Sbjct: 313 YPQGEPDAVVVRKQDIELLKPRRFINDTIIDFYIKYLKNRISPKERGRFHFFNCFFFRKL 372

Query: 386 VDLDKDLSSACGGRDAFQRVHKWTKKVNLFQKDYLFIPVNYSLHWSLVVICHPGEVINLK 445
            +LDK   S CGGR+A+QRV KWTK V+LF+KDY+FIP+N S HWSLV+ICHPGE++   
Sbjct: 373 ANLDKGTPSTCGGREAYQRVQKWTKNVDLFEKDYIFIPINCSFHWSLVIICHPGELV--- 432

Query: 446 DKKHDNLSKVPCILHMDSIKGSHR-GLKSLFQSYLCEEWKERYGDGDDKDISAVFLTLPF 505
               +N  +VPCILH+DSIKGSH+ GL ++F SYL EEWK R+ +  +    A    +  
Sbjct: 433 PSHVENPQRVPCILHLDSIKGSHKGGLINIFPSYLREEWKARHENTTNDSSRAP--NMQS 492

Query: 506 IPLELPQQENSFDCGLFLLHYVELFLEGAPVNFSSLKILKFSNFLSQDWFHPAEASLKRA 565
           I LELPQQENSFDCGLFLLHY++LF+  AP  F+   I + +NFL+++WF   EASLKR 
Sbjct: 493 ISLELPQQENSFDCGLFLLHYLDLFVAQAPAKFNPSLISRSANFLTRNWFPAKEASLKRR 540

Query: 566 HILKLIYEI 570
           +IL+L+Y +
Sbjct: 553 NILELLYNL 540

BLAST of Chy1G002190 vs. ExPASy Swiss-Prot
Match: Q8L7S0 (Probable ubiquitin-like-specific protease 2B OS=Arabidopsis thaliana OX=3702 GN=ULP2B PE=1 SV=3)

HSP 1 Score: 428.3 bits (1100), Expect = 1.9e-118
Identity = 261/659 (39.61%), Postives = 362/659 (54.93%), Query Frame = 0

Query: 26  KRFSVFDFSEEDARVEKVSRRLLGKFS--ARRSSPVTNHQFLHCF--------------- 85
           K F VFDF EED   E  + +LL KF+  +  +SPV   Q +  F               
Sbjct: 3   KNFEVFDFKEEDELAESAAGKLLEKFTNPSPCNSPVLQRQRIQSFCNEKRVEEEEMEGPS 62

Query: 86  ------------------------GKGAKSV---SRNLSDELIDIDAK-VGKSANIDSFS 145
                                    K ++++       +D L + DA+ V +   +   +
Sbjct: 63  CAEPATAVESDDHQCEDDSTLVTEAKESRTILTFGLETTDHLEETDAEHVNQGLMLGLKT 122

Query: 146 EDVSYELIHIDSEGHQISPGPTDLNAEDTCADTPLE----------GGGSMKQEIL---- 205
           ED++ E   ID + H +  G   LN+ED   +T ++          GG S   E      
Sbjct: 123 EDLAKE-TDIDHDNHGLMFG---LNSEDDIEETDVDHRVESFSCQLGGNSFYAETSSYSQ 182

Query: 206 --------------ETNDLL------------LSRSSTNEDD------------------ 265
                         E  D++            LS +S +EDD                  
Sbjct: 183 RQLNSPFSDSSSSEEQIDMMSAIDESLSDRSALSEASDSEDDEEDWMTEHCFNDEEKIDL 242

Query: 266 --VTVIFPDFVIYEGNWCTTSKLIFSCSCIKFRGSALSGLQRTFDSEWAISDIIGIESEW 325
               ++  ++VI +   C  S +IFSC+ IK +    +  +  F  E+ + DI+ I+  W
Sbjct: 243 STAVIMTSEYVILKDMHCAASLVIFSCNGIKIKSFLANNEEVPFSCEFGVEDIVSIQYNW 302

Query: 326 CSRVETAIVNLRLKGKHFTRAENSKDISGIELLKFSVCDPLWSESEKAIRTLNLRYNDLW 385
              V   I+ +R+      + EN  +   +E LK +V +  W   ++ I +L+++Y  +W
Sbjct: 303 YQNVGLIILRIRV----LLKDENCHE--DMEELKIAVKEHNWPNKQQKINSLHVKYPAVW 362

Query: 386 NADYDDNDKVNGEGIVSWRHSDVFSPNNCFSEFVDTFEEVIYPMGDPDAVTISKRDLELL 445
           N D +D+ +V+G  +   +          F  F + FE+V+YP GDPDAV+I KRD+ELL
Sbjct: 363 NTDLEDDVEVSGYNLNQQK--------RYFPSFDEPFEDVVYPKGDPDAVSICKRDVELL 422

Query: 446 KPGMFINDTIIDFYVKYLKNKFLLEKNNRFYFFNSFFFRKLVDLDKDLSSACGGRDAFQR 505
           +P  F+NDTIIDFY+ YLKN+   E+ +RF+FFNSFFFRKL DLDKD SS   G+ AF R
Sbjct: 423 QPETFVNDTIIDFYINYLKNQIQTEEKHRFHFFNSFFFRKLADLDKDPSSIADGKAAFLR 482

Query: 506 VHKWTKKVNLFQKDYLFIPVNYSLHWSLVVICHPGEVINLKDKKHDNLSKVPCILHMDSI 565
           V KWT+KV++F KDY+F+PVNY+LHWSL+VICHPGEV N  D   D+  KVPCILHMDSI
Sbjct: 483 VRKWTRKVDMFGKDYIFVPVNYNLHWSLIVICHPGEVANRTDLDLDDSKKVPCILHMDSI 542

Query: 566 KGSHRGLKSLFQSYLCEEWKERYGDGDDKDISAVFLTLPFIPLELPQQENSFDCGLFLLH 580
           KGSH GLK+L Q+YLCEEWKER+ +  D DIS+ F+ L F+ LELPQQENSFDCGLFLLH
Sbjct: 543 KGSHAGLKNLVQTYLCEEWKERHKETSD-DISSRFMNLRFVSLELPQQENSFDCGLFLLH 602

BLAST of Chy1G002190 vs. ExPASy Swiss-Prot
Match: Q8RWN0 (Ubiquitin-like-specific protease 1C OS=Arabidopsis thaliana OX=3702 GN=ULP1C PE=1 SV=1)

HSP 1 Score: 140.2 bits (352), Expect = 1.0e-31
Identity = 93/266 (34.96%), Postives = 147/266 (55.26%), Query Frame = 0

Query: 318 EEVIYPMGDP----DAVTISKRDLELLKPGMFINDTIIDFYVKYLKNK-FLLEKN-NRFY 377
           E++ YP  D     D V +S +DL+ L PG ++   +I+FY++Y+++  F  +K     +
Sbjct: 315 EDIYYPSSDQSDGRDLVQVSLKDLKCLSPGEYLTSPVINFYIRYVQHHVFSADKTAANCH 374

Query: 378 FFNSFFFRKLVDLDKDLSSACGGRDA-FQRVHKWTKKVNLFQKDYLFIPVNYSLHWSLVV 437
           FFN+FF++KL +    +S     RDA F +  +W K  +LF K Y+FIP++  LHWSLV+
Sbjct: 375 FFNTFFYKKLTEA---VSYKGNDRDAYFVKFRRWWKGFDLFCKSYIFIPIHEDLHWSLVI 434

Query: 438 ICHPGEVINLKDKKHDNLSKVPCILHMDSIKGSHRGL-KSLFQSYLCEEWKERYGDGD-D 497
           IC P       DK+ ++      I+H+DS+    R L  +  + +L EEW     D   D
Sbjct: 435 ICIP-------DKEDES---GLTIIHLDSLGLHPRNLIFNNVKRFLREEWNYLNQDAPLD 494

Query: 498 KDISA-VFLTLPFI----PLELPQQENSFDCGLFLLHYVELFLEGAPVNFSSLKILKFSN 557
             ISA V+  LP +     +++PQQ+N FDCGLFLL ++  F+E AP   +    L+   
Sbjct: 495 LPISAKVWRDLPNMINEAEVQVPQQKNDFDCGLFLLFFIRRFIEEAPQRLT----LQDLK 554

Query: 558 FLSQDWFHPAEASLKRAHILKLIYEI 570
            + + WF P EAS  R  I  ++ ++
Sbjct: 555 MIHKKWFKPEEASALRIKIWNILVDL 563

BLAST of Chy1G002190 vs. ExPASy Swiss-Prot
Match: O13769 (Ubiquitin-like-specific protease 2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=ulp2 PE=1 SV=2)

HSP 1 Score: 138.3 bits (347), Expect = 3.9e-31
Identity = 97/310 (31.29%), Postives = 146/310 (47.10%), Query Frame = 0

Query: 320 VIYPMGDPDAVTISKRDLELLKPGMFINDTIIDFYVKYLKNKFLLEK---NNRFYFFNSF 379
           ++YP    +++ I+  DL  L  G F+NDTI+DFY++YL  K   +     N  + FN+F
Sbjct: 337 LVYPFSGTNSIAITNTDLTRLNEGEFLNDTIVDFYLRYLYCKLQTQNPSLANDTHIFNTF 396

Query: 380 FFRKLVDLDKDLSSACGGRDAFQRVHKWTKKVNLFQKDYLFIPVNYSLHWSLVVICHPGE 439
           F+ +L   DKD     G R   + V KWT+KV+LF K Y+ +P+N + HW L +IC+   
Sbjct: 397 FYNRLTSKDKD-----GKRLGHRGVRKWTQKVDLFHKKYIIVPINETFHWYLAIICNIDR 456

Query: 440 VINL---------------------KDKKHDNLSKVPCILHMDSIKGSHRGLKSLFQSYL 499
           ++ +                     K ++ +  S  P IL  DS+   H+G  +  + YL
Sbjct: 457 LMPVDTKLEEQDEIVMSSVEQPSASKTRQAELTSNSPAILIFDSLANLHKGALNYLREYL 516

Query: 500 CEEWKERYGDGDDKDISAVFLTLPFIPLELPQQENSFDCGLFLLHYVELFLEG-----AP 559
            EE  ER      K++      +     ++PQQ N  DCG++ LH+VELFLE      A 
Sbjct: 517 LEEAFER------KNVHLKSTDIRGFHAKVPQQSNFSDCGIYALHFVELFLETPEQVIAN 576

Query: 560 VNFSSLKILKFSNFLSQDW----FHPAEASLKRAHILKLIYEIMVCNQAKELSGSIGKYP 597
               SL+     NF  Q W     +     LK   I +L  E    N+ + LS       
Sbjct: 577 TLDKSLRRTDAKNF-DQQWNLQKINTMRCDLK-GLIRRLSTEWSSNNERQSLSSG----- 628

BLAST of Chy1G002190 vs. ExPASy Swiss-Prot
Match: Q2PS26 (Ubiquitin-like-specific protease 1D OS=Arabidopsis thaliana OX=3702 GN=ULP1D PE=1 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 2.2e-29
Identity = 84/266 (31.58%), Postives = 141/266 (53.01%), Query Frame = 0

Query: 318 EEVIYP-MGDPDAVTISKRDLELLKPGMFINDTIIDFYVKYLKNKFLLEKNNRF----YF 377
           E++ YP   DP  V +  +DLE L P  ++   +++FY+++L+ +  +  +N+     +F
Sbjct: 330 EDICYPTRDDPHFVQVCLKDLECLAPREYLTSPVMNFYMRFLQQQ--ISSSNQISADCHF 389

Query: 378 FNSFFFRKLVDLDKDLSSACGGRDA-FQRVHKWTKKVNLFQKDYLFIPVNYSLHWSLVVI 437
           FN++F++KL D    ++     +DA F R  +W K ++LF+K Y+FIP++  LHWSLV++
Sbjct: 390 FNTYFYKKLSDA---VTYKGNDKDAFFVRFRRWWKGIDLFRKAYIFIPIHEDLHWSLVIV 449

Query: 438 CHPGEVINLKDKKHDNLSKVPCILHMDSI-KGSHRGLKSLFQSYLCEEWKERYGDGDDKD 497
           C P       DKK ++      ILH+DS+   S + +    + +L +EW     D    D
Sbjct: 450 CIP-------DKKDES---GLTILHLDSLGLHSRKSIVENVKRFLKDEWNYLNQDDYSLD 509

Query: 498 I---SAVFLTLP----FIPLELPQQENSFDCGLFLLHYVELFLEGAPVNFSSLKILKFSN 557
           +     V+  LP       +++PQQ+N FDCG F+L +++ F+E AP         K   
Sbjct: 510 LPISEKVWKNLPRRISEAVVQVPQQKNDFDCGPFVLFFIKRFIEEAPQRLKR----KDLG 569

Query: 558 FLSQDWFHPAEASLKRAHILKLIYEI 570
              + WF P EAS  R  I   + E+
Sbjct: 570 MFDKKWFRPDEASALRIKIRNTLIEL 576

BLAST of Chy1G002190 vs. ExPASy TrEMBL
Match: A0A0A0K633 (ULP_PROTEASE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G065160 PE=3 SV=1)

HSP 1 Score: 1493.4 bits (3865), Expect = 0.0e+00
Identity = 756/814 (92.87%), Postives = 766/814 (94.10%), Query Frame = 0

Query: 1   MTRTSSSKPFPSTRRKGRGRGDGGGKRFSVFDFSEEDARVEKVSRRLLGKFSARRSSPVT 60
           MTRTSSSKPFPSTRRK R  GDGGGKRFSVFDFSEEDARVEKVSRRLLGKFSARRSSPVT
Sbjct: 1   MTRTSSSKPFPSTRRKERDIGDGGGKRFSVFDFSEEDARVEKVSRRLLGKFSARRSSPVT 60

Query: 61  NHQFLHCFGKGAKSVSRNLSDELIDIDAKVGKSANIDSFSEDVSYELIHIDSEGHQISPG 120
            HQFLHCFGKGAKSVSRNLSDELIDIDA+VGKSANIDSFSEDVS ELIHIDSEGHQISPG
Sbjct: 61  KHQFLHCFGKGAKSVSRNLSDELIDIDAEVGKSANIDSFSEDVSDELIHIDSEGHQISPG 120

Query: 121 PTDLNAEDTCADTPLEGGGSMKQEILETNDLLLSRSSTNEDDVTVIFPDFVIYEGNWCTT 180
           PTDLNAEDTCADTPLEGGGSMKQEILETND+LLSRSSTNEDDVTVIFPDFVIYEGNWCTT
Sbjct: 121 PTDLNAEDTCADTPLEGGGSMKQEILETNDILLSRSSTNEDDVTVIFPDFVIYEGNWCTT 180

Query: 181 SKLIFSCSCIKFRGSALSGLQRTFDSEWAISDIIGIESEWCSRVETAIVNLRLKGKHFTR 240
           SKLIFSCSCIKFRGSALSGLQRTFDSEWAISDIIGIESEWCSRVETAIVNL LKGKHFTR
Sbjct: 181 SKLIFSCSCIKFRGSALSGLQRTFDSEWAISDIIGIESEWCSRVETAIVNLCLKGKHFTR 240

Query: 241 AENSKDISGIELLKFSVCDPLWSESEKAIRTLNLRYNDLWNADYDDNDKVNGEGIVSWRH 300
           AENSKDISGIELLKFSVCDPLWSESEKAIRTLNLRYNDLWNAD+DDNDKVNGE IVSWRH
Sbjct: 241 AENSKDISGIELLKFSVCDPLWSESEKAIRTLNLRYNDLWNADHDDNDKVNGEEIVSWRH 300

Query: 301 SDVFSPNNCFSEFVDTFEEVIYPMGDPDAVTISKRDLELLKPGMFINDTIIDFYVKYLKN 360
           SDVFSP NCFSEFVDTFEEVIYPMGDPDAVTISKRDLELLKPGMFINDTIIDFYVKYLKN
Sbjct: 301 SDVFSPKNCFSEFVDTFEEVIYPMGDPDAVTISKRDLELLKPGMFINDTIIDFYVKYLKN 360

Query: 361 KFLLEKNNRFYFFNSFFFRKLVDLDKDLSSACGGRDAFQRVHKWTKKVNLFQKDYLFIPV 420
           KFL EKNNRFYFFNSFFFRKLVDLDKDLSSA GGRDAFQRVHKWTKKVNLFQKDYLFIPV
Sbjct: 361 KFLSEKNNRFYFFNSFFFRKLVDLDKDLSSARGGRDAFQRVHKWTKKVNLFQKDYLFIPV 420

Query: 421 NYSLHWSLVVICHPGEVINLKDKKHDNLSKVPCILHMDSIKGSHRGLKSLFQSYLCEEWK 480
           NYSLHWSLVVICHPGEV+NLKDKKHDNLSKVPCILHMDSIKGSHRGLKSLFQSYLCEEWK
Sbjct: 421 NYSLHWSLVVICHPGEVVNLKDKKHDNLSKVPCILHMDSIKGSHRGLKSLFQSYLCEEWK 480

Query: 481 ERYGDGDDKDISAVFLTLPFIPLELPQQENSFDCGLFLLHYVELFLEGAPVNFSSLKILK 540
           ERYGDGD KDISAVFLTLPFIPLELPQQENSFDCGLFLLHYVELFLEGAPVNFSSLKILK
Sbjct: 481 ERYGDGDYKDISAVFLTLPFIPLELPQQENSFDCGLFLLHYVELFLEGAPVNFSSLKILK 540

Query: 541 FSNFLSQDWFHPAEASLKRAHILKLIYEIMVCNQAKELSGSIGKYPSSDANDSDNDLSKH 600
           FSNFLSQDWFHPAEASLKRAHILKLIYEIM CNQAKELSGSIGKYPSSDANDSDNDLSKH
Sbjct: 541 FSNFLSQDWFHPAEASLKRAHILKLIYEIMACNQAKELSGSIGKYPSSDANDSDNDLSKH 600

Query: 601 VSGEAHIFTMTHSDNFINVGKEFGSVSKVSSDTNYQRIGRWESVMPPIEEDENGEIADSP 660
           VSG+AHIFTMTHSDNF +VGKE GSVSKVSSDTNYQ IGRWESVMPPIEEDENGE ADSP
Sbjct: 601 VSGQAHIFTMTHSDNFSSVGKEVGSVSKVSSDTNYQPIGRWESVMPPIEEDENGERADSP 660

Query: 661 QSLEDRSQASAVSECSSAFSFGQQFTELEISWEGRYSKNVKDMCRKPSPRPSLHELQTPL 720
           Q LEDR QAS VSECSSAFSFGQQFTELEI WEGRYSKNVK+MCRKPSPR SLHELQTPL
Sbjct: 661 QCLEDRPQASTVSECSSAFSFGQQFTELEICWEGRYSKNVKEMCRKPSPRLSLHELQTPL 720

Query: 721 ELGQDCTPEATKNHNHHHPSEPDNQPQILTSSSDELINCVVEDSEEEGNERNDRIEIQV- 780
           ELG                     QP+ILTSSSDELINCVVEDSEEEGNERN+RIEIQV 
Sbjct: 721 ELG---------------------QPEILTSSSDELINCVVEDSEEEGNERNERIEIQVS 780

Query: 781 -SSSSSSRNNLFLSRQVVESPANFSDNNRQHEHK 813
            SSSSSSRNNLFLSRQVVESPA FS   RQH+HK
Sbjct: 781 SSSSSSSRNNLFLSRQVVESPAKFS---RQHQHK 790

BLAST of Chy1G002190 vs. ExPASy TrEMBL
Match: A0A5A7SPN7 (Putative ubiquitin-like-specific protease 2A isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold139G002040 PE=3 SV=1)

HSP 1 Score: 1478.4 bits (3826), Expect = 0.0e+00
Identity = 749/821 (91.23%), Postives = 767/821 (93.42%), Query Frame = 0

Query: 1   MTRTSSSKPFPSTRRKGRGRGDGGGKRFSVFDFSEEDARVEKVSRRLLGKFSARRSSPVT 60
           MTRTSSSKPF STRR GRGRG+GGGKRFSVFDFSEED RVEKVSR LLGKFSARRSSPVT
Sbjct: 1   MTRTSSSKPFSSTRRNGRGRGEGGGKRFSVFDFSEEDVRVEKVSRSLLGKFSARRSSPVT 60

Query: 61  NHQFLHCFGKGAKSVSRNLSDELIDIDAKVGKSANIDSFSEDVSYELIHIDSE------- 120
            HQFLHCFGKGAKSVSRNLSDELIDIDA+VGK AN DSFSED+SYELIHIDSE       
Sbjct: 61  KHQFLHCFGKGAKSVSRNLSDELIDIDAEVGKGANTDSFSEDISYELIHIDSEVSISTLN 120

Query: 121 -GHQISPGPTDLNAEDTCADTPLEGGGSMKQEILETNDLLLSRSSTNEDDVTVIFPDFVI 180
             HQISPGP DLNAEDTCAD  LEGGGSMKQEILETNDLL SRSSTNEDD TVIFPDFVI
Sbjct: 121 TSHQISPGPIDLNAEDTCADGSLEGGGSMKQEILETNDLLRSRSSTNEDDFTVIFPDFVI 180

Query: 181 YEGNWCTTSKLIFSCSCIKFRGSALSGLQRTFDSEWAISDIIGIESEWCSRVETAIVNLR 240
           YEGNWCTTSKLIFSCSCIKF+GSALSGLQRTFDSEWA+SDIIGIESEWCSRVETAIVNLR
Sbjct: 181 YEGNWCTTSKLIFSCSCIKFQGSALSGLQRTFDSEWAVSDIIGIESEWCSRVETAIVNLR 240

Query: 241 LKGKHFTRAENSKDISGIELLKFSVCDPLWSESEKAIRTLNLRYNDLWNADYDDNDKVNG 300
           LKGKHFT AENS DISGIELLKFSVCDPLWSESEKAIRTLN+RYNDLWNADYDDNDKV  
Sbjct: 241 LKGKHFTGAENSNDISGIELLKFSVCDPLWSESEKAIRTLNVRYNDLWNADYDDNDKVKW 300

Query: 301 EGIVSWRHSDVFSPNNCFSEFVDTFEEVIYPMGDPDAVTISKRDLELLKPGMFINDTIID 360
           E IVSWRHSDVF P NCFSEFVDTFEEVIYP GDPDAVTISKRDLELLKPGMFINDTIID
Sbjct: 301 EEIVSWRHSDVFFPKNCFSEFVDTFEEVIYPKGDPDAVTISKRDLELLKPGMFINDTIID 360

Query: 361 FYVKYLKNKFLLEKNNRFYFFNSFFFRKLVDLDKDLSSACGGRDAFQRVHKWTKKVNLFQ 420
           FYVKYLKNKFL EKN+RFYFFNSFFFRKL DLDKDLSSACGGRDAFQRVHKWTKKVNLFQ
Sbjct: 361 FYVKYLKNKFLSEKNDRFYFFNSFFFRKLADLDKDLSSACGGRDAFQRVHKWTKKVNLFQ 420

Query: 421 KDYLFIPVNYSLHWSLVVICHPGEVINLKDKKHDNLSKVPCILHMDSIKGSHRGLKSLFQ 480
           KDYLFIPVNYSLHWSLVVICHPGEV+NLKDKKHDNLSKVPCILHMDSIKGSHRGLKSLFQ
Sbjct: 421 KDYLFIPVNYSLHWSLVVICHPGEVVNLKDKKHDNLSKVPCILHMDSIKGSHRGLKSLFQ 480

Query: 481 SYLCEEWKERYGDG-DDKDISAVFLTLPFIPLELPQQENSFDCGLFLLHYVELFLEGAPV 540
           SYLCEEWKERYGDG DD+DISAVFLTLPFIPLELPQQENSFDCGLFLLHYVELFLEGAPV
Sbjct: 481 SYLCEEWKERYGDGDDDEDISAVFLTLPFIPLELPQQENSFDCGLFLLHYVELFLEGAPV 540

Query: 541 NFSSLKILKFSNFLSQDWFHPAEASLKRAHILKLIYEIMVCNQAKELSGSIGKYPSSDAN 600
           NFS LKILK SNFLSQDWFHPAEASLKRAHILKLIYEIMVCNQAKELSGS+GKYPSSDAN
Sbjct: 541 NFSPLKILKLSNFLSQDWFHPAEASLKRAHILKLIYEIMVCNQAKELSGSVGKYPSSDAN 600

Query: 601 DSDNDLSKHVSGEAHIFTMTHSDNFINVGKEFGSVSKVSSDTNYQRIGRWESVMPPIEED 660
           DSDNDLSKHVSGEA IFTMTHSDNF +VGKEFGSVSKVSSDTNYQRIG  ESVMPPIEED
Sbjct: 601 DSDNDLSKHVSGEADIFTMTHSDNFSSVGKEFGSVSKVSSDTNYQRIGGRESVMPPIEED 660

Query: 661 ENGEIADSPQSLEDRSQASAVSECSSAFSFGQQFTELEISWEGRYSKNVK-DMCRKPSPR 720
           ENGE ADSPQ L+DR QASAV E SSAFSFGQQFTELEISWEGRYS+NVK DMCRKPSPR
Sbjct: 661 ENGETADSPQCLDDRLQASAVFEFSSAFSFGQQFTELEISWEGRYSQNVKEDMCRKPSPR 720

Query: 721 PSLHELQTPLELGQDCTPEATKNHNHHHPSEPDNQPQILTSSSDELINCVVEDSEEEGNE 780
           PSLHELQT L+LGQD TP+ATKN N  HP+E DNQ +ILTSSSDELINCVVEDSEEEGNE
Sbjct: 721 PSLHELQTTLKLGQDSTPQATKNPN--HPTEADNQLEILTSSSDELINCVVEDSEEEGNE 780

Query: 781 RNDRIEIQVSSSSSSRNNLFLSRQVVESPANFSDNNRQHEH 812
           RNDRIEI+V  SSSSRNNLFLSRQVVES ANFSDNNRQHEH
Sbjct: 781 RNDRIEIEV--SSSSRNNLFLSRQVVESTANFSDNNRQHEH 817

BLAST of Chy1G002190 vs. ExPASy TrEMBL
Match: A0A1S3C1G7 (probable ubiquitin-like-specific protease 2A isoform X2 OS=Cucumis melo OX=3656 GN=LOC103495387 PE=3 SV=1)

HSP 1 Score: 1478.4 bits (3826), Expect = 0.0e+00
Identity = 749/821 (91.23%), Postives = 767/821 (93.42%), Query Frame = 0

Query: 1   MTRTSSSKPFPSTRRKGRGRGDGGGKRFSVFDFSEEDARVEKVSRRLLGKFSARRSSPVT 60
           MTRTSSSKPF STRR GRGRG+GGGKRFSVFDFSEED RVEKVSR LLGKFSARRSSPVT
Sbjct: 1   MTRTSSSKPFSSTRRNGRGRGEGGGKRFSVFDFSEEDVRVEKVSRSLLGKFSARRSSPVT 60

Query: 61  NHQFLHCFGKGAKSVSRNLSDELIDIDAKVGKSANIDSFSEDVSYELIHIDSE------- 120
            HQFLHCFGKGAKSVSRNLSDELIDIDA+VGK AN DSFSED+SYELIHIDSE       
Sbjct: 61  KHQFLHCFGKGAKSVSRNLSDELIDIDAEVGKGANTDSFSEDISYELIHIDSEVSISTLN 120

Query: 121 -GHQISPGPTDLNAEDTCADTPLEGGGSMKQEILETNDLLLSRSSTNEDDVTVIFPDFVI 180
             HQISPGP DLNAEDTCAD  LEGGGSMKQEILETNDLL SRSSTNEDD TVIFPDFVI
Sbjct: 121 TSHQISPGPIDLNAEDTCADGSLEGGGSMKQEILETNDLLRSRSSTNEDDFTVIFPDFVI 180

Query: 181 YEGNWCTTSKLIFSCSCIKFRGSALSGLQRTFDSEWAISDIIGIESEWCSRVETAIVNLR 240
           YEGNWCTTSKLIFSCSCIKF+GSALSGLQRTFDSEWA+SDIIGIESEWCSRVETAIVNLR
Sbjct: 181 YEGNWCTTSKLIFSCSCIKFQGSALSGLQRTFDSEWAVSDIIGIESEWCSRVETAIVNLR 240

Query: 241 LKGKHFTRAENSKDISGIELLKFSVCDPLWSESEKAIRTLNLRYNDLWNADYDDNDKVNG 300
           LKGKHFT AENS DISGIELLKFSVCDPLWSESEKAIRTLN+RYNDLWNADYDDNDKV  
Sbjct: 241 LKGKHFTGAENSNDISGIELLKFSVCDPLWSESEKAIRTLNVRYNDLWNADYDDNDKVKW 300

Query: 301 EGIVSWRHSDVFSPNNCFSEFVDTFEEVIYPMGDPDAVTISKRDLELLKPGMFINDTIID 360
           E IVSWRHSDVF P NCFSEFVDTFEEVIYP GDPDAVTISKRDLELLKPGMFINDTIID
Sbjct: 301 EEIVSWRHSDVFFPKNCFSEFVDTFEEVIYPKGDPDAVTISKRDLELLKPGMFINDTIID 360

Query: 361 FYVKYLKNKFLLEKNNRFYFFNSFFFRKLVDLDKDLSSACGGRDAFQRVHKWTKKVNLFQ 420
           FYVKYLKNKFL EKN+RFYFFNSFFFRKL DLDKDLSSACGGRDAFQRVHKWTKKVNLFQ
Sbjct: 361 FYVKYLKNKFLSEKNDRFYFFNSFFFRKLADLDKDLSSACGGRDAFQRVHKWTKKVNLFQ 420

Query: 421 KDYLFIPVNYSLHWSLVVICHPGEVINLKDKKHDNLSKVPCILHMDSIKGSHRGLKSLFQ 480
           KDYLFIPVNYSLHWSLVVICHPGEV+NLKDKKHDNLSKVPCILHMDSIKGSHRGLKSLFQ
Sbjct: 421 KDYLFIPVNYSLHWSLVVICHPGEVVNLKDKKHDNLSKVPCILHMDSIKGSHRGLKSLFQ 480

Query: 481 SYLCEEWKERYGDG-DDKDISAVFLTLPFIPLELPQQENSFDCGLFLLHYVELFLEGAPV 540
           SYLCEEWKERYGDG DD+DISAVFLTLPFIPLELPQQENSFDCGLFLLHYVELFLEGAPV
Sbjct: 481 SYLCEEWKERYGDGDDDEDISAVFLTLPFIPLELPQQENSFDCGLFLLHYVELFLEGAPV 540

Query: 541 NFSSLKILKFSNFLSQDWFHPAEASLKRAHILKLIYEIMVCNQAKELSGSIGKYPSSDAN 600
           NFS LKILK SNFLSQDWFHPAEASLKRAHILKLIYEIMVCNQAKELSGS+GKYPSSDAN
Sbjct: 541 NFSPLKILKLSNFLSQDWFHPAEASLKRAHILKLIYEIMVCNQAKELSGSVGKYPSSDAN 600

Query: 601 DSDNDLSKHVSGEAHIFTMTHSDNFINVGKEFGSVSKVSSDTNYQRIGRWESVMPPIEED 660
           DSDNDLSKHVSGEA IFTMTHSDNF +VGKEFGSVSKVSSDTNYQRIG  ESVMPPIEED
Sbjct: 601 DSDNDLSKHVSGEADIFTMTHSDNFSSVGKEFGSVSKVSSDTNYQRIGGRESVMPPIEED 660

Query: 661 ENGEIADSPQSLEDRSQASAVSECSSAFSFGQQFTELEISWEGRYSKNVK-DMCRKPSPR 720
           ENGE ADSPQ L+DR QASAV E SSAFSFGQQFTELEISWEGRYS+NVK DMCRKPSPR
Sbjct: 661 ENGETADSPQCLDDRLQASAVFEFSSAFSFGQQFTELEISWEGRYSQNVKEDMCRKPSPR 720

Query: 721 PSLHELQTPLELGQDCTPEATKNHNHHHPSEPDNQPQILTSSSDELINCVVEDSEEEGNE 780
           PSLHELQT L+LGQD TP+ATKN N  HP+E DNQ +ILTSSSDELINCVVEDSEEEGNE
Sbjct: 721 PSLHELQTTLKLGQDSTPQATKNPN--HPTEADNQLEILTSSSDELINCVVEDSEEEGNE 780

Query: 781 RNDRIEIQVSSSSSSRNNLFLSRQVVESPANFSDNNRQHEH 812
           RNDRIEI+V  SSSSRNNLFLSRQVVES ANFSDNNRQHEH
Sbjct: 781 RNDRIEIEV--SSSSRNNLFLSRQVVESTANFSDNNRQHEH 817

BLAST of Chy1G002190 vs. ExPASy TrEMBL
Match: A0A1S3C0Z2 (probable ubiquitin-like-specific protease 2A isoform X1 OS=Cucumis melo OX=3656 GN=LOC103495387 PE=3 SV=1)

HSP 1 Score: 1469.9 bits (3804), Expect = 0.0e+00
Identity = 749/832 (90.02%), Postives = 767/832 (92.19%), Query Frame = 0

Query: 1   MTRTSSSKPFPSTRRKGRGRGDGGGKRFSVFDFSEEDARVEKVSRRLLGKFSARRSSPVT 60
           MTRTSSSKPF STRR GRGRG+GGGKRFSVFDFSEED RVEKVSR LLGKFSARRSSPVT
Sbjct: 1   MTRTSSSKPFSSTRRNGRGRGEGGGKRFSVFDFSEEDVRVEKVSRSLLGKFSARRSSPVT 60

Query: 61  NHQFLHCFGKGAKSVSRNLSDELIDIDAKVGKSANIDSFSEDVSYELIHIDSE------- 120
            HQFLHCFGKGAKSVSRNLSDELIDIDA+VGK AN DSFSED+SYELIHIDSE       
Sbjct: 61  KHQFLHCFGKGAKSVSRNLSDELIDIDAEVGKGANTDSFSEDISYELIHIDSEVSISTLN 120

Query: 121 -GHQISPGPTDLNAEDTCADTPLEGGGSMKQEILETNDLLLSRSSTNEDDVTVIFPDFVI 180
             HQISPGP DLNAEDTCAD  LEGGGSMKQEILETNDLL SRSSTNEDD TVIFPDFVI
Sbjct: 121 TSHQISPGPIDLNAEDTCADGSLEGGGSMKQEILETNDLLRSRSSTNEDDFTVIFPDFVI 180

Query: 181 YEGNWCTTSKLIFSCSCIKFRGSALSGLQRTFDSEWAISDIIGIESEWCSRVETAIVNLR 240
           YEGNWCTTSKLIFSCSCIKF+GSALSGLQRTFDSEWA+SDIIGIESEWCSRVETAIVNLR
Sbjct: 181 YEGNWCTTSKLIFSCSCIKFQGSALSGLQRTFDSEWAVSDIIGIESEWCSRVETAIVNLR 240

Query: 241 LKGKHFTRAENSKDIS-----------GIELLKFSVCDPLWSESEKAIRTLNLRYNDLWN 300
           LKGKHFT AENS DIS           GIELLKFSVCDPLWSESEKAIRTLN+RYNDLWN
Sbjct: 241 LKGKHFTGAENSNDISGLFYVGIKVFMGIELLKFSVCDPLWSESEKAIRTLNVRYNDLWN 300

Query: 301 ADYDDNDKVNGEGIVSWRHSDVFSPNNCFSEFVDTFEEVIYPMGDPDAVTISKRDLELLK 360
           ADYDDNDKV  E IVSWRHSDVF P NCFSEFVDTFEEVIYP GDPDAVTISKRDLELLK
Sbjct: 301 ADYDDNDKVKWEEIVSWRHSDVFFPKNCFSEFVDTFEEVIYPKGDPDAVTISKRDLELLK 360

Query: 361 PGMFINDTIIDFYVKYLKNKFLLEKNNRFYFFNSFFFRKLVDLDKDLSSACGGRDAFQRV 420
           PGMFINDTIIDFYVKYLKNKFL EKN+RFYFFNSFFFRKL DLDKDLSSACGGRDAFQRV
Sbjct: 361 PGMFINDTIIDFYVKYLKNKFLSEKNDRFYFFNSFFFRKLADLDKDLSSACGGRDAFQRV 420

Query: 421 HKWTKKVNLFQKDYLFIPVNYSLHWSLVVICHPGEVINLKDKKHDNLSKVPCILHMDSIK 480
           HKWTKKVNLFQKDYLFIPVNYSLHWSLVVICHPGEV+NLKDKKHDNLSKVPCILHMDSIK
Sbjct: 421 HKWTKKVNLFQKDYLFIPVNYSLHWSLVVICHPGEVVNLKDKKHDNLSKVPCILHMDSIK 480

Query: 481 GSHRGLKSLFQSYLCEEWKERYGDG-DDKDISAVFLTLPFIPLELPQQENSFDCGLFLLH 540
           GSHRGLKSLFQSYLCEEWKERYGDG DD+DISAVFLTLPFIPLELPQQENSFDCGLFLLH
Sbjct: 481 GSHRGLKSLFQSYLCEEWKERYGDGDDDEDISAVFLTLPFIPLELPQQENSFDCGLFLLH 540

Query: 541 YVELFLEGAPVNFSSLKILKFSNFLSQDWFHPAEASLKRAHILKLIYEIMVCNQAKELSG 600
           YVELFLEGAPVNFS LKILK SNFLSQDWFHPAEASLKRAHILKLIYEIMVCNQAKELSG
Sbjct: 541 YVELFLEGAPVNFSPLKILKLSNFLSQDWFHPAEASLKRAHILKLIYEIMVCNQAKELSG 600

Query: 601 SIGKYPSSDANDSDNDLSKHVSGEAHIFTMTHSDNFINVGKEFGSVSKVSSDTNYQRIGR 660
           S+GKYPSSDANDSDNDLSKHVSGEA IFTMTHSDNF +VGKEFGSVSKVSSDTNYQRIG 
Sbjct: 601 SVGKYPSSDANDSDNDLSKHVSGEADIFTMTHSDNFSSVGKEFGSVSKVSSDTNYQRIGG 660

Query: 661 WESVMPPIEEDENGEIADSPQSLEDRSQASAVSECSSAFSFGQQFTELEISWEGRYSKNV 720
            ESVMPPIEEDENGE ADSPQ L+DR QASAV E SSAFSFGQQFTELEISWEGRYS+NV
Sbjct: 661 RESVMPPIEEDENGETADSPQCLDDRLQASAVFEFSSAFSFGQQFTELEISWEGRYSQNV 720

Query: 721 K-DMCRKPSPRPSLHELQTPLELGQDCTPEATKNHNHHHPSEPDNQPQILTSSSDELINC 780
           K DMCRKPSPRPSLHELQT L+LGQD TP+ATKN N  HP+E DNQ +ILTSSSDELINC
Sbjct: 721 KEDMCRKPSPRPSLHELQTTLKLGQDSTPQATKNPN--HPTEADNQLEILTSSSDELINC 780

Query: 781 VVEDSEEEGNERNDRIEIQVSSSSSSRNNLFLSRQVVESPANFSDNNRQHEH 812
           VVEDSEEEGNERNDRIEI+V  SSSSRNNLFLSRQVVES ANFSDNNRQHEH
Sbjct: 781 VVEDSEEEGNERNDRIEIEV--SSSSRNNLFLSRQVVESTANFSDNNRQHEH 828

BLAST of Chy1G002190 vs. ExPASy TrEMBL
Match: A0A6J1CJL7 (probable ubiquitin-like-specific protease 2A isoform X4 OS=Momordica charantia OX=3673 GN=LOC111012217 PE=3 SV=1)

HSP 1 Score: 1029.6 bits (2661), Expect = 6.9e-297
Identity = 566/872 (64.91%), Postives = 651/872 (74.66%), Query Frame = 0

Query: 12  STRRKGRGRGDGGGKRFSVFDFSEEDARVEKVSRRLLGKFSARRSSPVTNHQFLHCFGKG 71
           S+ R  +G G GG KRF VFDFS++D RVE+VS+ LLGKFS   SS VT +QFL CF KG
Sbjct: 8   SSSRSRKGGGAGGDKRFDVFDFSQDDHRVEEVSQSLLGKFSTGSSSAVTKYQFLQCFAKG 67

Query: 72  AKSVSRNLSDELIDIDAKVGKSANIDSFSEDVSYELIHIDSE------------------ 131
           A S+  N+S E IDIDA+V K A+ DS  EDVSYE+ HI+S+                  
Sbjct: 68  AASLRENVSVEFIDIDAEVEKGADTDSLCEDVSYEITHINSKVAKGAESLGNVSIELIDI 127

Query: 132 --------GHQISPGPTDLNAEDTCADTPLEGGGSMKQEILETNDLLLSRSSTNEDDVTV 191
                    HQIS GP+DL +EDTC D   E  G  ++EI ET+ +LLSR++ NEDDV V
Sbjct: 128 DAEVANSASHQISTGPSDLASEDTCVDRSPE-DGLKEREIFETSVVLLSRAANNEDDVIV 187

Query: 192 IFPDFVIYEGNWCTTSKLIFSCSCIKFRGSALS-GLQRTFDSEWAISDIIGIESEWCSRV 251
           IFPDFVIYEG  CTT+KLIFS SCIK +GS ++ G QRTFD +WAISDI+GIESEWC RV
Sbjct: 188 IFPDFVIYEGQCCTTAKLIFSRSCIKLQGSVVNGGWQRTFDCQWAISDIVGIESEWCRRV 247

Query: 252 ETAIVNLRLKGKHFTRAENSKDISGIELLKFSVCDPLWSESEKAIRTLNLRYNDLWNADY 311
           ETAIVNL LKGK+ TRA N+ +ISGIE +KFS+CDPLWSE EKAIR+LNL+YNDLWNA+Y
Sbjct: 248 ETAIVNLLLKGKNATRAGNANEISGIEPVKFSICDPLWSEREKAIRSLNLKYNDLWNANY 307

Query: 312 DDNDKVNGEGIVSWRHSDVFSPNNCFSEFVDTFEEVIYPMGDPDAVTISKRDLELLKPGM 371
           D  DK++ E IVS R SDVFSP +CF EF++ FEEVIYP GD DAVTI+KRDLELLKPG 
Sbjct: 308 D--DKIDWEEIVSRRQSDVFSPKHCFFEFIEPFEEVIYPKGDADAVTINKRDLELLKPGA 367

Query: 372 FINDTIIDFYVKYLKNKFLLEKNNRFYFFNSFFFRKLVDLDKDLSSACGGRDAFQRVHKW 431
           FINDTIIDFY+KYLKNKF LEKNNRFYFFNSFFFRKLVDLDKDLSSACGGR AFQRVHKW
Sbjct: 368 FINDTIIDFYIKYLKNKFQLEKNNRFYFFNSFFFRKLVDLDKDLSSACGGRAAFQRVHKW 427

Query: 432 TKKVNLFQKDYLFIPVNY----SLHWSLVVICHPGEVINLKDKKHDNLSKVPCILHMDSI 491
           TKKVNLF+KDY+FIP+NY    SLHWSLVVICHPGEV NLKDKK DNLSKVPCILH+DSI
Sbjct: 428 TKKVNLFEKDYIFIPINYSLDDSLHWSLVVICHPGEVGNLKDKKCDNLSKVPCILHLDSI 487

Query: 492 KGSHRGLKSLFQSYLCEEWKERYGDGDDKDISAVFLTLPFIPLELPQQENSFDCGLFLLH 551
           KGSHRGL +LFQ+YLCEEWKER+GDG D  ISA FL L F+PLELPQQENSFDCGLFLLH
Sbjct: 488 KGSHRGLMNLFQNYLCEEWKERHGDG-DVGISAKFLALQFVPLELPQQENSFDCGLFLLH 547

Query: 552 YVELFLEGAPVNFSSLKILKFSNFLSQDWFHPAEASLKRAHILKLIYEIMVCNQAKELSG 611
           YVE FLEGAPVNFS  KI +FSNFL+QDWF P EASLKRAHIL+LIY+IMV +  KE SG
Sbjct: 548 YVERFLEGAPVNFSPFKISRFSNFLNQDWFPPIEASLKRAHILQLIYDIMVNDHGKEFSG 607

Query: 612 SIGKYPSSDANDSDNDLSKHV-SGEAHIFTMTHSDNFINVGK------------------ 671
           SIGKYPSS    SD+DLS+HV   E H  T T SD F +  K                  
Sbjct: 608 SIGKYPSSIVGHSDSDLSRHVYLEEVHTSTTTCSDKFTSGEKEKENEMSTLLACPPKRFR 667

Query: 672 EFGSVSKVSSDTNYQRI-GRWESVMPPIEEDENGEIADSPQSLEDRSQASA-VSECSSAF 731
           E G VSKVSSDTNYQ+I G+  SVM PIEEDENGEI+DSPQ LEDR+ ASA VSECSSA 
Sbjct: 668 ELGLVSKVSSDTNYQQIGGQSRSVMSPIEEDENGEISDSPQCLEDRNHASAIVSECSSAS 727

Query: 732 SFGQQFTELEISWEGRYSKNVKDMCRKPSPRPSLHELQTPLELGQDCTPEATKNHNHHHP 791
           SFGQQF ELEIS EGR+S+N KD  R+PS +PSL E  T    G+D +P+A K  +  HP
Sbjct: 728 SFGQQFRELEISTEGRFSRNAKDKDRRPSSQPSLGESHTISVSGRDYSPQANKRLD--HP 787

Query: 792 SEPDNQPQILTSSSDELINCVVEDSEEE---------------------GNERNDRIEIQ 811
           +E D +P+ LT+SS+EL  CVVEDSEEE                     GN  +D  EI+
Sbjct: 788 TEAD-EPETLTTSSEELATCVVEDSEEEYIVEDSEEADEMYNGIEVVQSGNSMHDSKEIE 847

BLAST of Chy1G002190 vs. NCBI nr
Match: XP_011658819.1 (probable ubiquitin-like-specific protease 2A isoform X1 [Cucumis sativus] >KGN43757.1 hypothetical protein Csa_017116 [Cucumis sativus])

HSP 1 Score: 1490 bits (3858), Expect = 0.0
Identity = 756/814 (92.87%), Postives = 766/814 (94.10%), Query Frame = 0

Query: 1   MTRTSSSKPFPSTRRKGRGRGDGGGKRFSVFDFSEEDARVEKVSRRLLGKFSARRSSPVT 60
           MTRTSSSKPFPSTRRK R  GDGGGKRFSVFDFSEEDARVEKVSRRLLGKFSARRSSPVT
Sbjct: 1   MTRTSSSKPFPSTRRKERDIGDGGGKRFSVFDFSEEDARVEKVSRRLLGKFSARRSSPVT 60

Query: 61  NHQFLHCFGKGAKSVSRNLSDELIDIDAKVGKSANIDSFSEDVSYELIHIDSEGHQISPG 120
            HQFLHCFGKGAKSVSRNLSDELIDIDA+VGKSANIDSFSEDVS ELIHIDSEGHQISPG
Sbjct: 61  KHQFLHCFGKGAKSVSRNLSDELIDIDAEVGKSANIDSFSEDVSDELIHIDSEGHQISPG 120

Query: 121 PTDLNAEDTCADTPLEGGGSMKQEILETNDLLLSRSSTNEDDVTVIFPDFVIYEGNWCTT 180
           PTDLNAEDTCADTPLEGGGSMKQEILETND+LLSRSSTNEDDVTVIFPDFVIYEGNWCTT
Sbjct: 121 PTDLNAEDTCADTPLEGGGSMKQEILETNDILLSRSSTNEDDVTVIFPDFVIYEGNWCTT 180

Query: 181 SKLIFSCSCIKFRGSALSGLQRTFDSEWAISDIIGIESEWCSRVETAIVNLRLKGKHFTR 240
           SKLIFSCSCIKFRGSALSGLQRTFDSEWAISDIIGIESEWCSRVETAIVNL LKGKHFTR
Sbjct: 181 SKLIFSCSCIKFRGSALSGLQRTFDSEWAISDIIGIESEWCSRVETAIVNLCLKGKHFTR 240

Query: 241 AENSKDISGIELLKFSVCDPLWSESEKAIRTLNLRYNDLWNADYDDNDKVNGEGIVSWRH 300
           AENSKDISGIELLKFSVCDPLWSESEKAIRTLNLRYNDLWNAD+DDNDKVNGE IVSWRH
Sbjct: 241 AENSKDISGIELLKFSVCDPLWSESEKAIRTLNLRYNDLWNADHDDNDKVNGEEIVSWRH 300

Query: 301 SDVFSPNNCFSEFVDTFEEVIYPMGDPDAVTISKRDLELLKPGMFINDTIIDFYVKYLKN 360
           SDVFSP NCFSEFVDTFEEVIYPMGDPDAVTISKRDLELLKPGMFINDTIIDFYVKYLKN
Sbjct: 301 SDVFSPKNCFSEFVDTFEEVIYPMGDPDAVTISKRDLELLKPGMFINDTIIDFYVKYLKN 360

Query: 361 KFLLEKNNRFYFFNSFFFRKLVDLDKDLSSACGGRDAFQRVHKWTKKVNLFQKDYLFIPV 420
           KFL EKNNRFYFFNSFFFRKLVDLDKDLSSA GGRDAFQRVHKWTKKVNLFQKDYLFIPV
Sbjct: 361 KFLSEKNNRFYFFNSFFFRKLVDLDKDLSSARGGRDAFQRVHKWTKKVNLFQKDYLFIPV 420

Query: 421 NYSLHWSLVVICHPGEVINLKDKKHDNLSKVPCILHMDSIKGSHRGLKSLFQSYLCEEWK 480
           NYSLHWSLVVICHPGEV+NLKDKKHDNLSKVPCILHMDSIKGSHRGLKSLFQSYLCEEWK
Sbjct: 421 NYSLHWSLVVICHPGEVVNLKDKKHDNLSKVPCILHMDSIKGSHRGLKSLFQSYLCEEWK 480

Query: 481 ERYGDGDDKDISAVFLTLPFIPLELPQQENSFDCGLFLLHYVELFLEGAPVNFSSLKILK 540
           ERYGDGD KDISAVFLTLPFIPLELPQQENSFDCGLFLLHYVELFLEGAPVNFSSLKILK
Sbjct: 481 ERYGDGDYKDISAVFLTLPFIPLELPQQENSFDCGLFLLHYVELFLEGAPVNFSSLKILK 540

Query: 541 FSNFLSQDWFHPAEASLKRAHILKLIYEIMVCNQAKELSGSIGKYPSSDANDSDNDLSKH 600
           FSNFLSQDWFHPAEASLKRAHILKLIYEIM CNQAKELSGSIGKYPSSDANDSDNDLSKH
Sbjct: 541 FSNFLSQDWFHPAEASLKRAHILKLIYEIMACNQAKELSGSIGKYPSSDANDSDNDLSKH 600

Query: 601 VSGEAHIFTMTHSDNFINVGKEFGSVSKVSSDTNYQRIGRWESVMPPIEEDENGEIADSP 660
           VSG+AHIFTMTHSDNF +VGKE GSVSKVSSDTNYQ IGRWESVMPPIEEDENGE ADSP
Sbjct: 601 VSGQAHIFTMTHSDNFSSVGKEVGSVSKVSSDTNYQPIGRWESVMPPIEEDENGERADSP 660

Query: 661 QSLEDRSQASAVSECSSAFSFGQQFTELEISWEGRYSKNVKDMCRKPSPRPSLHELQTPL 720
           Q LEDR QAS VSECSSAFSFGQQFTELEI WEGRYSKNVK+MCRKPSPR SLHELQTPL
Sbjct: 661 QCLEDRPQASTVSECSSAFSFGQQFTELEICWEGRYSKNVKEMCRKPSPRLSLHELQTPL 720

Query: 721 ELGQDCTPEATKNHNHHHPSEPDNQPQILTSSSDELINCVVEDSEEEGNERNDRIEIQVS 780
           ELGQ                     P+ILTSSSDELINCVVEDSEEEGNERN+RIEIQVS
Sbjct: 721 ELGQ---------------------PEILTSSSDELINCVVEDSEEEGNERNERIEIQVS 780

Query: 781 SSSSS--RNNLFLSRQVVESPANFSDNNRQHEHK 812
           SSSSS  RNNLFLSRQVVESPA FS   RQH+HK
Sbjct: 781 SSSSSSSRNNLFLSRQVVESPAKFS---RQHQHK 790

BLAST of Chy1G002190 vs. NCBI nr
Match: XP_008455146.1 (PREDICTED: probable ubiquitin-like-specific protease 2A isoform X2 [Cucumis melo] >KAA0031447.1 putative ubiquitin-like-specific protease 2A isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 1476 bits (3821), Expect = 0.0
Identity = 749/821 (91.23%), Postives = 767/821 (93.42%), Query Frame = 0

Query: 1   MTRTSSSKPFPSTRRKGRGRGDGGGKRFSVFDFSEEDARVEKVSRRLLGKFSARRSSPVT 60
           MTRTSSSKPF STRR GRGRG+GGGKRFSVFDFSEED RVEKVSR LLGKFSARRSSPVT
Sbjct: 1   MTRTSSSKPFSSTRRNGRGRGEGGGKRFSVFDFSEEDVRVEKVSRSLLGKFSARRSSPVT 60

Query: 61  NHQFLHCFGKGAKSVSRNLSDELIDIDAKVGKSANIDSFSEDVSYELIHIDSE------- 120
            HQFLHCFGKGAKSVSRNLSDELIDIDA+VGK AN DSFSED+SYELIHIDSE       
Sbjct: 61  KHQFLHCFGKGAKSVSRNLSDELIDIDAEVGKGANTDSFSEDISYELIHIDSEVSISTLN 120

Query: 121 -GHQISPGPTDLNAEDTCADTPLEGGGSMKQEILETNDLLLSRSSTNEDDVTVIFPDFVI 180
             HQISPGP DLNAEDTCAD  LEGGGSMKQEILETNDLL SRSSTNEDD TVIFPDFVI
Sbjct: 121 TSHQISPGPIDLNAEDTCADGSLEGGGSMKQEILETNDLLRSRSSTNEDDFTVIFPDFVI 180

Query: 181 YEGNWCTTSKLIFSCSCIKFRGSALSGLQRTFDSEWAISDIIGIESEWCSRVETAIVNLR 240
           YEGNWCTTSKLIFSCSCIKF+GSALSGLQRTFDSEWA+SDIIGIESEWCSRVETAIVNLR
Sbjct: 181 YEGNWCTTSKLIFSCSCIKFQGSALSGLQRTFDSEWAVSDIIGIESEWCSRVETAIVNLR 240

Query: 241 LKGKHFTRAENSKDISGIELLKFSVCDPLWSESEKAIRTLNLRYNDLWNADYDDNDKVNG 300
           LKGKHFT AENS DISGIELLKFSVCDPLWSESEKAIRTLN+RYNDLWNADYDDNDKV  
Sbjct: 241 LKGKHFTGAENSNDISGIELLKFSVCDPLWSESEKAIRTLNVRYNDLWNADYDDNDKVKW 300

Query: 301 EGIVSWRHSDVFSPNNCFSEFVDTFEEVIYPMGDPDAVTISKRDLELLKPGMFINDTIID 360
           E IVSWRHSDVF P NCFSEFVDTFEEVIYP GDPDAVTISKRDLELLKPGMFINDTIID
Sbjct: 301 EEIVSWRHSDVFFPKNCFSEFVDTFEEVIYPKGDPDAVTISKRDLELLKPGMFINDTIID 360

Query: 361 FYVKYLKNKFLLEKNNRFYFFNSFFFRKLVDLDKDLSSACGGRDAFQRVHKWTKKVNLFQ 420
           FYVKYLKNKFL EKN+RFYFFNSFFFRKL DLDKDLSSACGGRDAFQRVHKWTKKVNLFQ
Sbjct: 361 FYVKYLKNKFLSEKNDRFYFFNSFFFRKLADLDKDLSSACGGRDAFQRVHKWTKKVNLFQ 420

Query: 421 KDYLFIPVNYSLHWSLVVICHPGEVINLKDKKHDNLSKVPCILHMDSIKGSHRGLKSLFQ 480
           KDYLFIPVNYSLHWSLVVICHPGEV+NLKDKKHDNLSKVPCILHMDSIKGSHRGLKSLFQ
Sbjct: 421 KDYLFIPVNYSLHWSLVVICHPGEVVNLKDKKHDNLSKVPCILHMDSIKGSHRGLKSLFQ 480

Query: 481 SYLCEEWKERYGDGDD-KDISAVFLTLPFIPLELPQQENSFDCGLFLLHYVELFLEGAPV 540
           SYLCEEWKERYGDGDD +DISAVFLTLPFIPLELPQQENSFDCGLFLLHYVELFLEGAPV
Sbjct: 481 SYLCEEWKERYGDGDDDEDISAVFLTLPFIPLELPQQENSFDCGLFLLHYVELFLEGAPV 540

Query: 541 NFSSLKILKFSNFLSQDWFHPAEASLKRAHILKLIYEIMVCNQAKELSGSIGKYPSSDAN 600
           NFS LKILK SNFLSQDWFHPAEASLKRAHILKLIYEIMVCNQAKELSGS+GKYPSSDAN
Sbjct: 541 NFSPLKILKLSNFLSQDWFHPAEASLKRAHILKLIYEIMVCNQAKELSGSVGKYPSSDAN 600

Query: 601 DSDNDLSKHVSGEAHIFTMTHSDNFINVGKEFGSVSKVSSDTNYQRIGRWESVMPPIEED 660
           DSDNDLSKHVSGEA IFTMTHSDNF +VGKEFGSVSKVSSDTNYQRIG  ESVMPPIEED
Sbjct: 601 DSDNDLSKHVSGEADIFTMTHSDNFSSVGKEFGSVSKVSSDTNYQRIGGRESVMPPIEED 660

Query: 661 ENGEIADSPQSLEDRSQASAVSECSSAFSFGQQFTELEISWEGRYSKNVK-DMCRKPSPR 720
           ENGE ADSPQ L+DR QASAV E SSAFSFGQQFTELEISWEGRYS+NVK DMCRKPSPR
Sbjct: 661 ENGETADSPQCLDDRLQASAVFEFSSAFSFGQQFTELEISWEGRYSQNVKEDMCRKPSPR 720

Query: 721 PSLHELQTPLELGQDCTPEATKNHNHHHPSEPDNQPQILTSSSDELINCVVEDSEEEGNE 780
           PSLHELQT L+LGQD TP+ATKN NH  P+E DNQ +ILTSSSDELINCVVEDSEEEGNE
Sbjct: 721 PSLHELQTTLKLGQDSTPQATKNPNH--PTEADNQLEILTSSSDELINCVVEDSEEEGNE 780

Query: 781 RNDRIEIQVSSSSSSRNNLFLSRQVVESPANFSDNNRQHEH 811
           RNDRIEI+VSSSS  RNNLFLSRQVVES ANFSDNNRQHEH
Sbjct: 781 RNDRIEIEVSSSS--RNNLFLSRQVVESTANFSDNNRQHEH 817

BLAST of Chy1G002190 vs. NCBI nr
Match: XP_008455144.1 (PREDICTED: probable ubiquitin-like-specific protease 2A isoform X1 [Cucumis melo])

HSP 1 Score: 1467 bits (3799), Expect = 0.0
Identity = 749/832 (90.02%), Postives = 767/832 (92.19%), Query Frame = 0

Query: 1   MTRTSSSKPFPSTRRKGRGRGDGGGKRFSVFDFSEEDARVEKVSRRLLGKFSARRSSPVT 60
           MTRTSSSKPF STRR GRGRG+GGGKRFSVFDFSEED RVEKVSR LLGKFSARRSSPVT
Sbjct: 1   MTRTSSSKPFSSTRRNGRGRGEGGGKRFSVFDFSEEDVRVEKVSRSLLGKFSARRSSPVT 60

Query: 61  NHQFLHCFGKGAKSVSRNLSDELIDIDAKVGKSANIDSFSEDVSYELIHIDSE------- 120
            HQFLHCFGKGAKSVSRNLSDELIDIDA+VGK AN DSFSED+SYELIHIDSE       
Sbjct: 61  KHQFLHCFGKGAKSVSRNLSDELIDIDAEVGKGANTDSFSEDISYELIHIDSEVSISTLN 120

Query: 121 -GHQISPGPTDLNAEDTCADTPLEGGGSMKQEILETNDLLLSRSSTNEDDVTVIFPDFVI 180
             HQISPGP DLNAEDTCAD  LEGGGSMKQEILETNDLL SRSSTNEDD TVIFPDFVI
Sbjct: 121 TSHQISPGPIDLNAEDTCADGSLEGGGSMKQEILETNDLLRSRSSTNEDDFTVIFPDFVI 180

Query: 181 YEGNWCTTSKLIFSCSCIKFRGSALSGLQRTFDSEWAISDIIGIESEWCSRVETAIVNLR 240
           YEGNWCTTSKLIFSCSCIKF+GSALSGLQRTFDSEWA+SDIIGIESEWCSRVETAIVNLR
Sbjct: 181 YEGNWCTTSKLIFSCSCIKFQGSALSGLQRTFDSEWAVSDIIGIESEWCSRVETAIVNLR 240

Query: 241 LKGKHFTRAENSKDISG-----------IELLKFSVCDPLWSESEKAIRTLNLRYNDLWN 300
           LKGKHFT AENS DISG           IELLKFSVCDPLWSESEKAIRTLN+RYNDLWN
Sbjct: 241 LKGKHFTGAENSNDISGLFYVGIKVFMGIELLKFSVCDPLWSESEKAIRTLNVRYNDLWN 300

Query: 301 ADYDDNDKVNGEGIVSWRHSDVFSPNNCFSEFVDTFEEVIYPMGDPDAVTISKRDLELLK 360
           ADYDDNDKV  E IVSWRHSDVF P NCFSEFVDTFEEVIYP GDPDAVTISKRDLELLK
Sbjct: 301 ADYDDNDKVKWEEIVSWRHSDVFFPKNCFSEFVDTFEEVIYPKGDPDAVTISKRDLELLK 360

Query: 361 PGMFINDTIIDFYVKYLKNKFLLEKNNRFYFFNSFFFRKLVDLDKDLSSACGGRDAFQRV 420
           PGMFINDTIIDFYVKYLKNKFL EKN+RFYFFNSFFFRKL DLDKDLSSACGGRDAFQRV
Sbjct: 361 PGMFINDTIIDFYVKYLKNKFLSEKNDRFYFFNSFFFRKLADLDKDLSSACGGRDAFQRV 420

Query: 421 HKWTKKVNLFQKDYLFIPVNYSLHWSLVVICHPGEVINLKDKKHDNLSKVPCILHMDSIK 480
           HKWTKKVNLFQKDYLFIPVNYSLHWSLVVICHPGEV+NLKDKKHDNLSKVPCILHMDSIK
Sbjct: 421 HKWTKKVNLFQKDYLFIPVNYSLHWSLVVICHPGEVVNLKDKKHDNLSKVPCILHMDSIK 480

Query: 481 GSHRGLKSLFQSYLCEEWKERYGDGDD-KDISAVFLTLPFIPLELPQQENSFDCGLFLLH 540
           GSHRGLKSLFQSYLCEEWKERYGDGDD +DISAVFLTLPFIPLELPQQENSFDCGLFLLH
Sbjct: 481 GSHRGLKSLFQSYLCEEWKERYGDGDDDEDISAVFLTLPFIPLELPQQENSFDCGLFLLH 540

Query: 541 YVELFLEGAPVNFSSLKILKFSNFLSQDWFHPAEASLKRAHILKLIYEIMVCNQAKELSG 600
           YVELFLEGAPVNFS LKILK SNFLSQDWFHPAEASLKRAHILKLIYEIMVCNQAKELSG
Sbjct: 541 YVELFLEGAPVNFSPLKILKLSNFLSQDWFHPAEASLKRAHILKLIYEIMVCNQAKELSG 600

Query: 601 SIGKYPSSDANDSDNDLSKHVSGEAHIFTMTHSDNFINVGKEFGSVSKVSSDTNYQRIGR 660
           S+GKYPSSDANDSDNDLSKHVSGEA IFTMTHSDNF +VGKEFGSVSKVSSDTNYQRIG 
Sbjct: 601 SVGKYPSSDANDSDNDLSKHVSGEADIFTMTHSDNFSSVGKEFGSVSKVSSDTNYQRIGG 660

Query: 661 WESVMPPIEEDENGEIADSPQSLEDRSQASAVSECSSAFSFGQQFTELEISWEGRYSKNV 720
            ESVMPPIEEDENGE ADSPQ L+DR QASAV E SSAFSFGQQFTELEISWEGRYS+NV
Sbjct: 661 RESVMPPIEEDENGETADSPQCLDDRLQASAVFEFSSAFSFGQQFTELEISWEGRYSQNV 720

Query: 721 K-DMCRKPSPRPSLHELQTPLELGQDCTPEATKNHNHHHPSEPDNQPQILTSSSDELINC 780
           K DMCRKPSPRPSLHELQT L+LGQD TP+ATKN NH  P+E DNQ +ILTSSSDELINC
Sbjct: 721 KEDMCRKPSPRPSLHELQTTLKLGQDSTPQATKNPNH--PTEADNQLEILTSSSDELINC 780

Query: 781 VVEDSEEEGNERNDRIEIQVSSSSSSRNNLFLSRQVVESPANFSDNNRQHEH 811
           VVEDSEEEGNERNDRIEI+VSSSS  RNNLFLSRQVVES ANFSDNNRQHEH
Sbjct: 781 VVEDSEEEGNERNDRIEIEVSSSS--RNNLFLSRQVVESTANFSDNNRQHEH 828

BLAST of Chy1G002190 vs. NCBI nr
Match: XP_031744940.1 (probable ubiquitin-like-specific protease 2A isoform X2 [Cucumis sativus])

HSP 1 Score: 1390 bits (3599), Expect = 0.0
Identity = 716/814 (87.96%), Postives = 726/814 (89.19%), Query Frame = 0

Query: 1   MTRTSSSKPFPSTRRKGRGRGDGGGKRFSVFDFSEEDARVEKVSRRLLGKFSARRSSPVT 60
           MTRTSSSKPFPSTRRK R  GDGGGKRFSVFDFSEEDARVEKVSRRLLGKFSARRSSPVT
Sbjct: 1   MTRTSSSKPFPSTRRKERDIGDGGGKRFSVFDFSEEDARVEKVSRRLLGKFSARRSSPVT 60

Query: 61  NHQFLHCFGKGAKSVSRNLSDELIDIDAKVGKSANIDSFSEDVSYELIHIDSEGHQISPG 120
            HQFLHCFGKGAKSVSRNLSDELIDIDA+VGKSANIDSFSEDVS ELIHIDSEGHQISPG
Sbjct: 61  KHQFLHCFGKGAKSVSRNLSDELIDIDAEVGKSANIDSFSEDVSDELIHIDSEGHQISPG 120

Query: 121 PTDLNAEDTCADTPLEGGGSMKQEILETNDLLLSRSSTNEDDVTVIFPDFVIYEGNWCTT 180
           PTDLNAEDTCADTPLEGGGSMKQEILETND+LLSRSSTNEDDVTVIFPDFVIYEGNWCTT
Sbjct: 121 PTDLNAEDTCADTPLEGGGSMKQEILETNDILLSRSSTNEDDVTVIFPDFVIYEGNWCTT 180

Query: 181 SKLIFSCSCIKFRGSALSGLQRTFDSEWAISDIIGIESEWCSRVETAIVNLRLKGKHFTR 240
           SKLIFSCSCIKFRGSALSGLQRTFDSEWAISDIIGIESEWCSRVETAIVNL LKGKHFTR
Sbjct: 181 SKLIFSCSCIKFRGSALSGLQRTFDSEWAISDIIGIESEWCSRVETAIVNLCLKGKHFTR 240

Query: 241 AENSKDISGIELLKFSVCDPLWSESEKAIRTLNLRYNDLWNADYDDNDKVNGEGIVSWRH 300
           AENSKDISGIELLKFSVCDPLWSESEKAIRTLNLRYNDLWNAD+DDNDKVNGE IVSWRH
Sbjct: 241 AENSKDISGIELLKFSVCDPLWSESEKAIRTLNLRYNDLWNADHDDNDKVNGEEIVSWRH 300

Query: 301 SDVFSPNNCFSEFVDTFEEVIYPMGDPDAVTISKRDLELLKPGMFINDTIIDFYVKYLKN 360
           SDVFSP NCFSEFVDTFEEVIYPMGDPDAVTISKRDLELLKPGMFINDTIIDFYVKYLKN
Sbjct: 301 SDVFSPKNCFSEFVDTFEEVIYPMGDPDAVTISKRDLELLKPGMFINDTIIDFYVKYLKN 360

Query: 361 KFLLEKNNRFYFFNSFFFRKLVDLDKDLSSACGGRDAFQRVHKWTKKVNLFQKDYLFIPV 420
           KFL EKNNRFYFFNSFFFRKLVDLDKDLSSA GGRDAFQRVHKWTKKVNLFQKDYLFIPV
Sbjct: 361 KFLSEKNNRFYFFNSFFFRKLVDLDKDLSSARGGRDAFQRVHKWTKKVNLFQKDYLFIPV 420

Query: 421 NYSLHWSLVVICHPGEVINLKDKKHDNLSKVPCILHMDSIKGSHRGLKSLFQSYLCEEWK 480
           NYSLHWSLVVICHPGEV+NLKDKKHDNLSKVPCILHMDSIKGSHRGLKSLFQSYLCEEWK
Sbjct: 421 NYSLHWSLVVICHPGEVVNLKDKKHDNLSKVPCILHMDSIKGSHRGLKSLFQSYLCEEWK 480

Query: 481 ERYGDGDDKDISAVFLTLPFIPLELPQQENSFDCGLFLLHYVELFLEGAPVNFSSLKILK 540
           ERYGDGD KDISAVFLTLPFIPLEL                                   
Sbjct: 481 ERYGDGDYKDISAVFLTLPFIPLEL----------------------------------- 540

Query: 541 FSNFLSQDWFHPAEASLKRAHILKLIYEIMVCNQAKELSGSIGKYPSSDANDSDNDLSKH 600
                SQDWFHPAEASLKRAHILKLIYEIM CNQAKELSGSIGKYPSSDANDSDNDLSKH
Sbjct: 541 -----SQDWFHPAEASLKRAHILKLIYEIMACNQAKELSGSIGKYPSSDANDSDNDLSKH 600

Query: 601 VSGEAHIFTMTHSDNFINVGKEFGSVSKVSSDTNYQRIGRWESVMPPIEEDENGEIADSP 660
           VSG+AHIFTMTHSDNF +VGKE GSVSKVSSDTNYQ IGRWESVMPPIEEDENGE ADSP
Sbjct: 601 VSGQAHIFTMTHSDNFSSVGKEVGSVSKVSSDTNYQPIGRWESVMPPIEEDENGERADSP 660

Query: 661 QSLEDRSQASAVSECSSAFSFGQQFTELEISWEGRYSKNVKDMCRKPSPRPSLHELQTPL 720
           Q LEDR QAS VSECSSAFSFGQQFTELEI WEGRYSKNVK+MCRKPSPR SLHELQTPL
Sbjct: 661 QCLEDRPQASTVSECSSAFSFGQQFTELEICWEGRYSKNVKEMCRKPSPRLSLHELQTPL 720

Query: 721 ELGQDCTPEATKNHNHHHPSEPDNQPQILTSSSDELINCVVEDSEEEGNERNDRIEIQVS 780
           ELGQ                     P+ILTSSSDELINCVVEDSEEEGNERN+RIEIQVS
Sbjct: 721 ELGQ---------------------PEILTSSSDELINCVVEDSEEEGNERNERIEIQVS 750

Query: 781 SSSSS--RNNLFLSRQVVESPANFSDNNRQHEHK 812
           SSSSS  RNNLFLSRQVVESPA FS   RQH+HK
Sbjct: 781 SSSSSSSRNNLFLSRQVVESPAKFS---RQHQHK 750

BLAST of Chy1G002190 vs. NCBI nr
Match: XP_038888439.1 (probable ubiquitin-like-specific protease 2A isoform X1 [Benincasa hispida])

HSP 1 Score: 1313 bits (3397), Expect = 0.0
Identity = 680/821 (82.83%), Postives = 720/821 (87.70%), Query Frame = 0

Query: 1   MTRTSSSKPFPSTRRKGRGRGDGGGKRFSVFDFSEEDARVEKVSRRLLGKFSARRSSPVT 60
           MTRTS  KP  S  RKGR RGDGGGKRFSVFDFSEED RVEKVSR LLGKFSAR+SSPV 
Sbjct: 1   MTRTS--KPSSSRSRKGRERGDGGGKRFSVFDFSEEDDRVEKVSRSLLGKFSARKSSPVA 60

Query: 61  NHQFLHCFGKGAKSVSRNLSDELIDIDAKVGKSANIDSFSEDVSYELIHIDSE------- 120
            HQFL CF KGA+SVSRNLSD  I IDA+VGKSAN DSFSEDVS ELIH DSE       
Sbjct: 61  KHQFLQCFAKGAESVSRNLSDVFIVIDAEVGKSANTDSFSEDVSNELIHNDSEVSISTLN 120

Query: 121 -GHQISPGPTDLNAEDTCADTPLEGGGSMKQEILETNDLLLSRSSTNEDDVTVIFPDFVI 180
             HQISPGPTDL+ EDTCAD  LEG GS KQEI ETND LLS SSTNEDDVTV+FPDFVI
Sbjct: 121 TSHQISPGPTDLDTEDTCADRSLEGSGSTKQEIFETNDQLLSCSSTNEDDVTVVFPDFVI 180

Query: 181 YEGNWCTTSKLIFSCSCIKFRGSALSGLQRTFDSEWAISDIIGIESEWCSRVETAIVNLR 240
           YEGNWCTTSKLIFSC+CIK +GSA+SGLQRTFDSEWA+SD++GIESEWCSRVETAIVNLR
Sbjct: 181 YEGNWCTTSKLIFSCTCIKLQGSAVSGLQRTFDSEWAVSDVVGIESEWCSRVETAIVNLR 240

Query: 241 LKGKHFTRAENSKDISGIELLKFSVCDPLWSESEKAIRTLNLRYNDLWNADYDDNDKVNG 300
           LKGKH TRA NS DISGIELLKFSVCDPLWSE EKAIRTLNLRYNDLWNADYDD   +  
Sbjct: 241 LKGKHCTRAANSNDISGIELLKFSVCDPLWSEREKAIRTLNLRYNDLWNADYDD--MIKW 300

Query: 301 EGIVSWRHSDVFSPNNCFSEFVDTFEEVIYPMGDPDAVTISKRDLELLKPGMFINDTIID 360
           E IVSWR SDVFS  +CFSEFVDTFEEVIYP GDPDAVTISKRDLELLKP  FINDTIID
Sbjct: 301 EEIVSWRQSDVFSLKHCFSEFVDTFEEVIYPEGDPDAVTISKRDLELLKPETFINDTIID 360

Query: 361 FYVKYLKNKFLLEKNNRFYFFNSFFFRKLVDLDKDLSSACGGRDAFQRVHKWTKKVNLFQ 420
           FYVKYLKNKFL EKNNRFYFFNSFFFRKLVDLDK+LSSACGGRDA+QRVHKWTKKVNLFQ
Sbjct: 361 FYVKYLKNKFLPEKNNRFYFFNSFFFRKLVDLDKNLSSACGGRDAYQRVHKWTKKVNLFQ 420

Query: 421 KDYLFIPVNYSLHWSLVVICHPGEVINLKDKKHDNLSKVPCILHMDSIKGSHRGLKSLFQ 480
           KDY+FIPVNYSLHWSLVVICHPGEV+NLKDKK DNLSKVPCILHMDSIKGSHRGLK+LFQ
Sbjct: 421 KDYIFIPVNYSLHWSLVVICHPGEVVNLKDKKRDNLSKVPCILHMDSIKGSHRGLKNLFQ 480

Query: 481 SYLCEEWKERYGDGDD-KDISAVFLTLPFIPLELPQQENSFDCGLFLLHYVELFLEGAPV 540
           +YLCEEWKERYGDGDD +D+SA+FLTLPF PLELPQQENSFDCGLFLLHYVELFLEGAPV
Sbjct: 481 NYLCEEWKERYGDGDDDEDVSAMFLTLPFFPLELPQQENSFDCGLFLLHYVELFLEGAPV 540

Query: 541 NFSSLKILKFSNFLSQDWFHPAEASLKRAHILKLIYEIMVCNQAKELSGSIGKYPSSDAN 600
           NF+ LKILKFSNFLSQ+WFHP E SLKRAHIL+LIYEIMV NQAKELSGSIGKYPSSDA+
Sbjct: 541 NFNPLKILKFSNFLSQNWFHPMEVSLKRAHILQLIYEIMVSNQAKELSGSIGKYPSSDAS 600

Query: 601 DSDNDLSKHVSGEAHIFTMTHSDNFINVGKEFGSVSKVSSDTNYQRIGRWE-SVMPPIEE 660
           DSD        GEAHIFTMTHSDNF +VGKEFGSV KVSSDTNYQRIGR   SVMPPIEE
Sbjct: 601 DSD-------LGEAHIFTMTHSDNFSSVGKEFGSVYKVSSDTNYQRIGRQSGSVMPPIEE 660

Query: 661 DENGEIAD-SPQSLEDRSQASAVSECSSAFSFGQQFTELEISWEGRYSKNVKDMCRKPSP 720
           +E GEIAD SPQ LEDR QASAVSECSSAFSFGQQFTELEISWEGR+S+N+KD  R PSP
Sbjct: 661 EEKGEIADESPQCLEDRHQASAVSECSSAFSFGQQFTELEISWEGRFSRNIKDTGRTPSP 720

Query: 721 RPSLHELQTPLELGQDCTPEATKNHNHHHPSEPDNQPQILTSSSDELINCVVEDSEEEGN 780
           RP   E QT  ELG+DCTP+ TK+ NH  P+E DN+P ILTSSS+EL+ CVVEDSEEEGN
Sbjct: 721 RPLHRESQTTFELGRDCTPQETKSLNH--PTEADNEPLILTSSSEELVTCVVEDSEEEGN 780

Query: 781 ERNDRIEIQVSSSSSSRNNLFLSRQVVESPANFSDNNRQHE 810
           ERND IEI VSSSS  RNNLFLS+QVVES AN SDN RQH+
Sbjct: 781 ERNDGIEIDVSSSS--RNNLFLSKQVVESTANSSDN-RQHD 805

BLAST of Chy1G002190 vs. TAIR 10
Match: AT4G33620.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 465.3 bits (1196), Expect = 1.0e-130
Identity = 244/555 (43.96%), Postives = 345/555 (62.16%), Query Frame = 0

Query: 26  KRFSVFDFSEEDARVEKVSRRLLGKFSA----RRSSPVTNHQFLHCFGKGAKSVSRNLSD 85
           K   VFD+S+ED RVE+ S++LL KF +    +    +  ++FL CF K  +S S+ L  
Sbjct: 13  KPIDVFDYSDEDDRVEEESKKLLRKFDSPVTKKHHCAIDKYEFLRCFAKDTQSESKVLQH 72

Query: 86  ELIDIDAKVGKSANIDSFSEDVSYELIHIDSEGHQISPGPTDLNAEDTCADTPLEGGGSM 145
            +ID++  V +  +    S D + +LI + S G     G   L +     +  +  G + 
Sbjct: 73  IVIDVEVPVKEEPSRCELSGDGNSDLIDVISNGSHRRIGIDSLTSSSLSENDEVSTGEA- 132

Query: 146 KQEILETNDLLLSRSSTNEDDVTVIFPDFVIYEGNWCTTSKLIFSCSCIKFRGSALSGLQ 205
                 TN          E+   +I PD +IY   +CT SKL FS +C+    S+++  +
Sbjct: 133 ------TNPASDPHEVDPENAQVLIIPDVIIYGDIYCTNSKLTFSRNCMNVESSSVNATK 192

Query: 206 RTFDSEWAISDIIGIESEWCSRVETAIVNLRLKGKHFTRAENSKDISGIELLKFSVCDPL 265
            TF  +W I DII IES+WC  VETA VN+ LK +     + +KDISGI+LLKFSV DP 
Sbjct: 193 GTFSCQWTIEDIIKIESQWCLEVETAFVNVLLKSRKPEGVDIAKDISGIDLLKFSVYDPK 252

Query: 266 WSESEKAIRTLNLRYNDLWNADYDDNDKVNGEGIVSWRHSDVFSPNNCFSEFVDTFEEVI 325
           WS+  + IR+L+ RY ++W     +++++   G                +   D+FE+++
Sbjct: 253 WSKEVETIRSLDSRYKNIWFDTITESEEIAFSG---------HDLGTSLTNLADSFEDLV 312

Query: 326 YPMGDPDAVTISKRDLELLKPGMFINDTIIDFYVKYLKNKFLLEKNNRFYFFNSFFFRKL 385
           YP G+PDAV + K+D+ELLKP  FINDTIIDFY+KYLKN+   ++  RF+FFN FFFRKL
Sbjct: 313 YPQGEPDAVVVRKQDIELLKPRRFINDTIIDFYIKYLKNRISPKERGRFHFFNCFFFRKL 372

Query: 386 VDLDKDLSSACGGRDAFQRVHKWTKKVNLFQKDYLFIPVNYSLHWSLVVICHPGEVI--- 445
            +LDK   S CGGR+A+QRV KWTK V+LF+KDY+FIP+N S HWSLV+ICHPGE++   
Sbjct: 373 ANLDKGTPSTCGGREAYQRVQKWTKNVDLFEKDYIFIPINCSFHWSLVIICHPGELVPSH 432

Query: 446 ---NLKDKKHDNLSKVPCILHMDSIKGSHR-GLKSLFQSYLCEEWKERYGDGDDKDISAV 505
              +  D + +N  +VPCILH+DSIKGSH+ GL ++F SYL EEWK R+ +  +    A 
Sbjct: 433 VNFHSFDDEVENPQRVPCILHLDSIKGSHKGGLINIFPSYLREEWKARHENTTNDSSRAP 492

Query: 506 FLTLPFIPLELPQQENSFDCGLFLLHYVELFLEGAPVNFSSLKILKFSNFLSQDWFHPAE 565
              +  I LELPQQENSFDCGLFLLHY++LF+  AP  F+   I + +NFL+++WF   E
Sbjct: 493 --NMQSISLELPQQENSFDCGLFLLHYLDLFVAQAPAKFNPSLISRSANFLTRNWFPAKE 549

Query: 566 ASLKRAHILKLIYEI 570
           ASLKR +IL+L+Y +
Sbjct: 553 ASLKRRNILELLYNL 549

BLAST of Chy1G002190 vs. TAIR 10
Match: AT1G09730.2 (Cysteine proteinases superfamily protein )

HSP 1 Score: 428.3 bits (1100), Expect = 1.4e-119
Identity = 261/659 (39.61%), Postives = 362/659 (54.93%), Query Frame = 0

Query: 26  KRFSVFDFSEEDARVEKVSRRLLGKFS--ARRSSPVTNHQFLHCF--------------- 85
           K F VFDF EED   E  + +LL KF+  +  +SPV   Q +  F               
Sbjct: 3   KNFEVFDFKEEDELAESAAGKLLEKFTNPSPCNSPVLQRQRIQSFCNEKRVEEEEMEGPS 62

Query: 86  ------------------------GKGAKSV---SRNLSDELIDIDAK-VGKSANIDSFS 145
                                    K ++++       +D L + DA+ V +   +   +
Sbjct: 63  CAEPATAVESDDHQCEDDSTLVTEAKESRTILTFGLETTDHLEETDAEHVNQGLMLGLKT 122

Query: 146 EDVSYELIHIDSEGHQISPGPTDLNAEDTCADTPLE----------GGGSMKQEIL---- 205
           ED++ E   ID + H +  G   LN+ED   +T ++          GG S   E      
Sbjct: 123 EDLAKE-TDIDHDNHGLMFG---LNSEDDIEETDVDHRVESFSCQLGGNSFYAETSSYSQ 182

Query: 206 --------------ETNDLL------------LSRSSTNEDD------------------ 265
                         E  D++            LS +S +EDD                  
Sbjct: 183 RQLNSPFSDSSSSEEQIDMMSAIDESLSDRSALSEASDSEDDEEDWMTEHCFNDEEKIDL 242

Query: 266 --VTVIFPDFVIYEGNWCTTSKLIFSCSCIKFRGSALSGLQRTFDSEWAISDIIGIESEW 325
               ++  ++VI +   C  S +IFSC+ IK +    +  +  F  E+ + DI+ I+  W
Sbjct: 243 STAVIMTSEYVILKDMHCAASLVIFSCNGIKIKSFLANNEEVPFSCEFGVEDIVSIQYNW 302

Query: 326 CSRVETAIVNLRLKGKHFTRAENSKDISGIELLKFSVCDPLWSESEKAIRTLNLRYNDLW 385
              V   I+ +R+      + EN  +   +E LK +V +  W   ++ I +L+++Y  +W
Sbjct: 303 YQNVGLIILRIRV----LLKDENCHE--DMEELKIAVKEHNWPNKQQKINSLHVKYPAVW 362

Query: 386 NADYDDNDKVNGEGIVSWRHSDVFSPNNCFSEFVDTFEEVIYPMGDPDAVTISKRDLELL 445
           N D +D+ +V+G  +   +          F  F + FE+V+YP GDPDAV+I KRD+ELL
Sbjct: 363 NTDLEDDVEVSGYNLNQQK--------RYFPSFDEPFEDVVYPKGDPDAVSICKRDVELL 422

Query: 446 KPGMFINDTIIDFYVKYLKNKFLLEKNNRFYFFNSFFFRKLVDLDKDLSSACGGRDAFQR 505
           +P  F+NDTIIDFY+ YLKN+   E+ +RF+FFNSFFFRKL DLDKD SS   G+ AF R
Sbjct: 423 QPETFVNDTIIDFYINYLKNQIQTEEKHRFHFFNSFFFRKLADLDKDPSSIADGKAAFLR 482

Query: 506 VHKWTKKVNLFQKDYLFIPVNYSLHWSLVVICHPGEVINLKDKKHDNLSKVPCILHMDSI 565
           V KWT+KV++F KDY+F+PVNY+LHWSL+VICHPGEV N  D   D+  KVPCILHMDSI
Sbjct: 483 VRKWTRKVDMFGKDYIFVPVNYNLHWSLIVICHPGEVANRTDLDLDDSKKVPCILHMDSI 542

Query: 566 KGSHRGLKSLFQSYLCEEWKERYGDGDDKDISAVFLTLPFIPLELPQQENSFDCGLFLLH 580
           KGSH GLK+L Q+YLCEEWKER+ +  D DIS+ F+ L F+ LELPQQENSFDCGLFLLH
Sbjct: 543 KGSHAGLKNLVQTYLCEEWKERHKETSD-DISSRFMNLRFVSLELPQQENSFDCGLFLLH 602

BLAST of Chy1G002190 vs. TAIR 10
Match: AT1G09730.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 422.5 bits (1085), Expect = 7.4e-118
Identity = 241/561 (42.96%), Postives = 333/561 (59.36%), Query Frame = 0

Query: 34  SEEDARVEKVSRRLLGKFSARRSSPVTNHQFLHCFGKGAKSVSRNLSDELIDIDAKVGKS 93
           ++ D RVE  S +L G      +S  +  Q    F   +       S+E ID+ + + +S
Sbjct: 151 TDVDHRVESFSCQLGGNSFYAETSSYSQRQLNSPFSDSSS------SEEQIDMMSAIDES 210

Query: 94  ANIDSFSEDVSYELIHIDSEGHQISPGPTDLNAEDTCADTPLEG-----GGSMKQEIL-- 153
            +  S   + S      DSE  +          E TC    L       G  M   +L  
Sbjct: 211 LSDRSALSEAS------DSEDDE----------EGTCYTFSLYFLIDMLGSPMSDRVLIS 270

Query: 154 ---ETNDLLLSRSSTNEDDV-----TVIFPDFVIYEGNWCTTSKLIFSCSCIKFRGSALS 213
                 D +      +E+ +      ++  ++VI +   C  S +IFSC+ IK +    +
Sbjct: 271 MLYALKDWMTEHCFNDEEKIDLSTAVIMTSEYVILKDMHCAASLVIFSCNGIKIKSFLAN 330

Query: 214 GLQRTFDSEWAISDIIGIESEWCSRVETAIVNLRLKGKHFTRAENSKDISGIELLKFSVC 273
             +  F  E+ + DI+ I+  W   V   I+ +R+      + EN  +   +E LK +V 
Sbjct: 331 NEEVPFSCEFGVEDIVSIQYNWYQNVGLIILRIRV----LLKDENCHE--DMEELKIAVK 390

Query: 274 DPLWSESEKAIRTLNLRYNDLWNADYDDNDKVNGEGIVSWRHSDVFSPNNCFSEFVDTFE 333
           +  W   ++ I +L+++Y  +WN D +D+ +V+G  +   +          F  F + FE
Sbjct: 391 EHNWPNKQQKINSLHVKYPAVWNTDLEDDVEVSGYNLNQQK--------RYFPSFDEPFE 450

Query: 334 EVIYPMGDPDAVTISKRDLELLKPGMFINDTIIDFYVKYLKNKFLLEKNNRFYFFNSFFF 393
           +V+YP GDPDAV+I KRD+ELL+P  F+NDTIIDFY+ YLKN+   E+ +RF+FFNSFFF
Sbjct: 451 DVVYPKGDPDAVSICKRDVELLQPETFVNDTIIDFYINYLKNQIQTEEKHRFHFFNSFFF 510

Query: 394 RKLVDLDKDLSSACGGRDAFQRVHKWTKKVNLFQKDYLFIPVNYSLHWSLVVICHPGEVI 453
           RKL DLDKD SS   G+ AF RV KWT+KV++F KDY+F+PVNY+LHWSL+VICHPGEV 
Sbjct: 511 RKLADLDKDPSSIADGKAAFLRVRKWTRKVDMFGKDYIFVPVNYNLHWSLIVICHPGEVA 570

Query: 454 NLKDKKHDNLSKVPCILHMDSIKGSHRGLKSLFQSYLCEEWKERYGDGDDKDISAVFLTL 513
           N  D   D+  KVPCILHMDSIKGSH GLK+L Q+YLCEEWKER+ +  D DIS+ F+ L
Sbjct: 571 NRTDLDLDDSKKVPCILHMDSIKGSHAGLKNLVQTYLCEEWKERHKETSD-DISSRFMNL 630

Query: 514 PFIPLELPQQENSFDCGLFLLHYVELFLEGAPVNFSSLKILKFSNFLSQDWFHPAEASLK 573
            F+ LELPQQENSFDCGLFLLHY+ELFL  AP+NFS  KI   SNFL  +WF PAEASLK
Sbjct: 631 RFVSLELPQQENSFDCGLFLLHYLELFLAEAPLNFSPFKIYNASNFLYLNWFPPAEASLK 673

Query: 574 RAHILKLIYEIMVCNQAKELS 580
           R  I KLI+E++  N+++E+S
Sbjct: 691 RTLIQKLIFELLE-NRSREVS 673

BLAST of Chy1G002190 vs. TAIR 10
Match: AT1G10570.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 140.2 bits (352), Expect = 7.4e-33
Identity = 93/266 (34.96%), Postives = 147/266 (55.26%), Query Frame = 0

Query: 318 EEVIYPMGDP----DAVTISKRDLELLKPGMFINDTIIDFYVKYLKNK-FLLEKN-NRFY 377
           E++ YP  D     D V +S +DL+ L PG ++   +I+FY++Y+++  F  +K     +
Sbjct: 315 EDIYYPSSDQSDGRDLVQVSLKDLKCLSPGEYLTSPVINFYIRYVQHHVFSADKTAANCH 374

Query: 378 FFNSFFFRKLVDLDKDLSSACGGRDA-FQRVHKWTKKVNLFQKDYLFIPVNYSLHWSLVV 437
           FFN+FF++KL +    +S     RDA F +  +W K  +LF K Y+FIP++  LHWSLV+
Sbjct: 375 FFNTFFYKKLTEA---VSYKGNDRDAYFVKFRRWWKGFDLFCKSYIFIPIHEDLHWSLVI 434

Query: 438 ICHPGEVINLKDKKHDNLSKVPCILHMDSIKGSHRGL-KSLFQSYLCEEWKERYGDGD-D 497
           IC P       DK+ ++      I+H+DS+    R L  +  + +L EEW     D   D
Sbjct: 435 ICIP-------DKEDES---GLTIIHLDSLGLHPRNLIFNNVKRFLREEWNYLNQDAPLD 494

Query: 498 KDISA-VFLTLPFI----PLELPQQENSFDCGLFLLHYVELFLEGAPVNFSSLKILKFSN 557
             ISA V+  LP +     +++PQQ+N FDCGLFLL ++  F+E AP   +    L+   
Sbjct: 495 LPISAKVWRDLPNMINEAEVQVPQQKNDFDCGLFLLFFIRRFIEEAPQRLT----LQDLK 554

Query: 558 FLSQDWFHPAEASLKRAHILKLIYEI 570
            + + WF P EAS  R  I  ++ ++
Sbjct: 555 MIHKKWFKPEEASALRIKIWNILVDL 563

BLAST of Chy1G002190 vs. TAIR 10
Match: AT1G10570.2 (Cysteine proteinases superfamily protein )

HSP 1 Score: 140.2 bits (352), Expect = 7.4e-33
Identity = 93/266 (34.96%), Postives = 147/266 (55.26%), Query Frame = 0

Query: 318 EEVIYPMGDP----DAVTISKRDLELLKPGMFINDTIIDFYVKYLKNK-FLLEKN-NRFY 377
           E++ YP  D     D V +S +DL+ L PG ++   +I+FY++Y+++  F  +K     +
Sbjct: 314 EDIYYPSSDQSDGRDLVQVSLKDLKCLSPGEYLTSPVINFYIRYVQHHVFSADKTAANCH 373

Query: 378 FFNSFFFRKLVDLDKDLSSACGGRDA-FQRVHKWTKKVNLFQKDYLFIPVNYSLHWSLVV 437
           FFN+FF++KL +    +S     RDA F +  +W K  +LF K Y+FIP++  LHWSLV+
Sbjct: 374 FFNTFFYKKLTEA---VSYKGNDRDAYFVKFRRWWKGFDLFCKSYIFIPIHEDLHWSLVI 433

Query: 438 ICHPGEVINLKDKKHDNLSKVPCILHMDSIKGSHRGL-KSLFQSYLCEEWKERYGDGD-D 497
           IC P       DK+ ++      I+H+DS+    R L  +  + +L EEW     D   D
Sbjct: 434 ICIP-------DKEDES---GLTIIHLDSLGLHPRNLIFNNVKRFLREEWNYLNQDAPLD 493

Query: 498 KDISA-VFLTLPFI----PLELPQQENSFDCGLFLLHYVELFLEGAPVNFSSLKILKFSN 557
             ISA V+  LP +     +++PQQ+N FDCGLFLL ++  F+E AP   +    L+   
Sbjct: 494 LPISAKVWRDLPNMINEAEVQVPQQKNDFDCGLFLLFFIRRFIEEAPQRLT----LQDLK 553

Query: 558 FLSQDWFHPAEASLKRAHILKLIYEI 570
            + + WF P EAS  R  I  ++ ++
Sbjct: 554 MIHKKWFKPEEASALRIKIWNILVDL 562