Chy1G000990 (gene) Cucumber (hystrix) v1

Overview
NameChy1G000990
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionRNA pseudouridine synthase 4, mitochondrial
LocationchrH01: 642284 .. 646747 (+)
RNA-Seq ExpressionChy1G000990
SyntenyChy1G000990
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTAAATGTAGATGAAAATCATGAAACTTTTGAATTTGGGTTGAAGATGTTAGCATTGAAGTCTACTCCCAATTATGATGGGCATTGGGTAATATTATATCTTCTCTTCTTCTTCTGTTTTCTTTTGGAAATATCATAATTATTATTTCTATTTACATTGGGTAAAATGGTTAGTTTTTTCCTTGTTAGAAATAAGTTTGAATGCATTTAATATAGTTTTCATCCACTTCATGGGTTAAAAAAGGAAGACTGCACAGTGGTTGATTTTCTTTTTTTTCTTTGTCCTTTAATATTATAAAAAAATGCAATGGAAATATCTATTATTAATTTTAAACGAAATCAATTATTAAAATTGCCTTCACATCAATCAAATCATTTTAAGATCCGAATTGTGGGTTTTTAATTAGTGTCGTCTAATAATCAAGTTATTGAATCAAAATATATTATACTTCATACACTTTTTTTAATGTCCAAATCTAGCTTTTATGTTGTCAACAAATATAAATTTTAAATTAAGTAGTTGATTATGAAAAAACGAAGAAAAAAAAAAACATTTCCTTATCAACAATTTCAATAACTAAAATAAAATAATATGCAAAGTGTGGAGTGTTAATTCACTTTTGATCACTATGCTATTCCTAAAAAATAATTTCAAAAGTTTCTGTTTTAACTAATCTCTTAGTTTTTAATAATTGGACCTAAGTTGTTTTTAGGGGCAACTTATCAACTTTCCATTGCTTTATTATTATTTTTTTAAAGTTACTTCGTATATGTTAATAAGTTGCTTTCTATTTGTTACGCGTGCGATTGTTTTATATTTTATTAAATGGTTGATTTGACTATCTTAACTTCATTATTTAATCCTATTCAAATTTGTTAAAATGAATATGCAAAAGAAAAATATGAATACGAAAATGGTTACATTATTAAAACACACACTAAATTCTCAAAATTTTAAATAAAAATGATAAGAACTTCATATTTATTTCTACATGAAATGAAATGAATTATGACCTAACTTTTCAAAATAATGCAATCCTTACATAGAACAAAGATGGGCCATATCCAAAATATATATATATGTTGGGCCTTTGGCCCATTTCCTTGTTTTCGGTCTAAATTCAAAACCCCTAAATTTCGTAGTTCATTCCTTCACCTTTAGGCGCCACCCACCATGGGACTCCGGGCACTCTTCCGTCCTTCCATCATTCTCCGTACTTCACCGTCTTGCACTGATCCCACCGGCGAAATTCTCTATTTCTCTATTTTTCATGTGCTGAAGTCCACTTTAGCCTTGAGTAACGCCTCCGAACCCGTATCCCAACAGAAGAGTGAGAAGGGAAGTAATAGATGGTTCACGCTGCCTCCTTACACTGCGACTGTAAACGGTTCGATTTTTGGTAATCGCATAATGAACGGAGGAGCTCAAGCCGAATTAGGAGCTTCCACCGCTGCAATAACTGCTCTGAAATGGGTGATTCGTTGCTGCCCGGAATTGCCGAGGAGCCTCGTGCAAAAGCTGTTTCGACTGAGAAAGGTTCCGTCCTCAATTTCACTTAGTACTATGTTGAATTCTTGCAATTGAATAATCAGATAAGCTAACTGAAATGATGTCTTGAGATCTTTTTTCAATTGCTTGCCGTAGCAATAGCATGCAATTGAATGTTGGTGTGGCAATTCGCATCGCGTTGTTAAATTTAAGCATCTTCTTCAGTTTATAAATGTTTTCCTTTTGGTGTCTTTCAGGTTCGAAGAGGTTCTTCTATGATTGAAAATTCTGAATATAATTTGGAAGCGCGAGACCAACTTAAAAGAGTGAGCCTTTATCGTCTATACCTACACAGATATTAATTTATCTATTTATATTTAGATAACATTTGATTGTCATGTTTTCCACATTGAAAGGGAACTTCCTTACACCTTTACTAACATGTTTAAGTTATTGCAAGGTGGCAGCCAAGGACATTCTGAACGTAGGTGATCAAATTTTTCTCCCCATGTCAGTTCAAGTATTCTCCACAGAGAAAACTCGGGATTATTGCAATAAAGAGGAAGAGACATTCATGCATAGTCTAGTATTATACAAGGTTTTCACTTTTCCCTGTTCTTTTTTGTTGCCTGAGAGGAAGGTGGGGTTATGAACACTTAGAGGTACAGGTAATTGGGGCAGTAAGTTGATTATTATAGTTTGTGGGTTATAACTGCCTAATTTCGAGACCAAACAATATTCGTTTGTATTTTAATTTTCTCTGTTTGGAGTGCAATCTATTTCAATTAGAGTAGGAAATGGTATACATGGTAACAAAGAGAGAAAGAGAGGATGAAATAATAGACACTGTAACAAACATGAAACATCCCTTATGTTCCTCTTGCTGTTAGTGACACATTTTCTTTTTTCATTTCTTGGTATAAGGATCCAGCAATACTTGTTATCAATAAACCTCCTGGCTTGCCTGTACAGGTATTGTATTCAACTTGTACCATTGATCTATCCTTTTGAATGATCATGGTCTTAGTTTCCAAAATTCCAGTAAATAAAAGTGTTTCTGCAGGGTGGTATTGGTGTCAAAAGAAGTTTAGATGAATTAGCTGCAAGTTGTTTAAGTTATGGCTACTCAGAACCACCTCGACTAGTAAGTTTTTCTTTAATTGATAACCTCTGACTTTGTGAGTTTGTTTAACTACATTAAATAGTATGCCATATAACGATTTAAAAAAAGGCTGTCTTTTTTCCTTTTATGATGAGCATGTATTGCAATCTTTATCTTGTTATGGGCTTTGCTTATTTCTAGGTACACAGGCTAGACAGGGACAGCAGTGGCATCTTGATAATGGGCAGGTCTTCCACAAGTACTTCACTTCTTCATTCTGTCTTCCGTGAGAAAACTTTTGAGGCATCAAAACATGTAGTTTTCTCTGTACTCGTATTGTCTCTTAAGCAAAATGAAAATCTGATAGAAATAAAAACGCATTTAAAGCATCGTTATTGTTCTAACTGACGTGATTAATTCTTTTATTGTTCATTGCATTGAAGGCAAACCATGTTGATAGTGAGAAAAAGATTTTGCTAAAGAGGTATTGGGCACTTGTAATTGGTTCTCCTAGACGTTCAAAGGGGTTAATTACAGCTCCACTAGGAAAGGTTAGTGAATAATTTCTTTCAAGCAACTATCTTGAAAGTTTTATCCTATTCTGATTAACCATACATTTGCAATGGTTTCTTTATGAGTTAATAATCTGTTTTTTTTTCCCTTATATTTTCTCTTGTAATTTAGTGTGCCTATGATTTTAGATTGTCTTGGATGATCGAAGATCTGAGCAAATTATGGTCATTGATAATGCCGAACCTATGCCTTCTCAGCAAGCAATTACTCAATATAAAGTGATCAAATCTCATCATGGTTTGCATTTCACCCTTGCTAAATGAAGCATTTAACGTTTCATGTTGATTTTTCAAGCACTAGACGTAATTTTAATCCAAATATTGGAAGCACATTGGAACATAGTAGGAACATGCATTAAATTGTTTACTTTGGAGGTTTTTCGTTCTTTACATTACTTGGGCAGCTTGTATTAGCCGTTAGGCACATGAGATCATCTCTTTATCTTGTTCTAATAAAGCTTGCATCTGATCATTCGATTAGGAGGGGACTTTTTTTAATTGAGAGTAGAATATAACTCAGAACTTGTATACATTGTTATTCTTCCTTTTTAGTTCAGAATGAGAAAATTTGAATGCCTCCTAAATAGCTAGAATGCTTTATTTTCTAGTAGTTGTATATAATTCTACTTGCTTGACTTTTGATAGAAAGTCTGGCAAGTAAAGGAAAGAGCACTTTGCATGCCGAGAATTTACCATTTTAAATAAAGCATTTCGGTTAAAATTGATTAATTCATCTATAACATCAGAGCGCCTATCTTATCTATGCTATAAGATAAAATATCATATTCTGTTCACTTATTGCATGGCAATTGTTCAGGATACACTTGGTTGGAGTTGTGTCCCCTCACTGGAAGAAAACATCAGGTACCATCCAGCATCCATAGCTCCAACCACTACAAGTATCCATCTCGTTTTCTACAGATATTTGGCTGAACCTTTCCTTGTATGTGTTTAATGCAGCTTCGTGTACACTGTGCCGAGGTATTAGGAACGCCTATAGTTGGAGACTACAAATATGGATGGCAAGCACATAAAAATTGGAAACCATTTCGTTCTAATTTCCACAAGAATCAAGGAAAGAAACAATTCCTCCCTTTTGGTCTTGACTTTGAAAGTGGAAGTATTGCAGAAAATAATCCTCGTCTACACCTTCATTGTAAGCAACTGACTTTACCAGATGTTTCTTTTGCACTGCATAATGATTCTCTAGATCATGACTGCTCAAGATTGGAATGTCTGGAGTTGGATGCTCCTTTGCCTTCATACATGCAAAAAAGTTGGGATATCATAAACAGTTAG

mRNA sequence

ATGGTAAATGTAGATGAAAATCATGAAACTTTTGAATTTGGGTTGAAGATGTTAGCATTGAAGTCTACTCCCAATTATGATGGGCATTGGGCGCCACCCACCATGGGACTCCGGGCACTCTTCCGTCCTTCCATCATTCTCCGTACTTCACCGTCTTGCACTGATCCCACCGGCGAAATTCTCTATTTCTCTATTTTTCATGTGCTGAAGTCCACTTTAGCCTTGAGTAACGCCTCCGAACCCGTATCCCAACAGAAGAGTGAGAAGGGAAGTAATAGATGGTTCACGCTGCCTCCTTACACTGCGACTGTAAACGGTTCGATTTTTGGTAATCGCATAATGAACGGAGGAGCTCAAGCCGAATTAGGAGCTTCCACCGCTGCAATAACTGCTCTGAAATGGGTGATTCGTTGCTGCCCGGAATTGCCGAGGAGCCTCGTGCAAAAGCTGTTTCGACTGAGAAAGGTTCGAAGAGGTTCTTCTATGATTGAAAATTCTGAATATAATTTGGAAGCGCGAGACCAACTTAAAAGAGTGGCAGCCAAGGACATTCTGAACGTAGGTGATCAAATTTTTCTCCCCATGTCAGTTCAAGTATTCTCCACAGAGAAAACTCGGGATTATTGCAATAAAGAGGAAGAGACATTCATGCATAGTCTAGTATTATACAAGGATCCAGCAATACTTGTTATCAATAAACCTCCTGGCTTGCCTGTACAGGGTGGTATTGGTGTCAAAAGAAGTTTAGATGAATTAGCTGCAAGTTGTTTAAGTTATGGCTACTCAGAACCACCTCGACTAGTACACAGGCTAGACAGGGACAGCAGTGGCATCTTGATAATGGGCAGGTCTTCCACAAGTACTTCACTTCTTCATTCTGTCTTCCGTGAGAAAACTTTTGAGGCATCAAAACATGCAAACCATGTTGATAGTGAGAAAAAGATTTTGCTAAAGAGGTATTGGGCACTTGTAATTGGTTCTCCTAGACGTTCAAAGGGGTTAATTACAGCTCCACTAGGAAAGATTGTCTTGGATGATCGAAGATCTGAGCAAATTATGGTCATTGATAATGCCGAACCTATGCCTTCTCAGCAAGCAATTACTCAATATAAAGTGATCAAATCTCATCATGGATACACTTGGTTGGAGTTGTGTCCCCTCACTGGAAGAAAACATCAGCTTCGTGTACACTGTGCCGAGGTATTAGGAACGCCTATAGTTGGAGACTACAAATATGGATGGCAAGCACATAAAAATTGGAAACCATTTCGTTCTAATTTCCACAAGAATCAAGGAAAGAAACAATTCCTCCCTTTTGGTCTTGACTTTGAAAGTGGAAGTATTGCAGAAAATAATCCTCGTCTACACCTTCATTGTAAGCAACTGACTTTACCAGATGTTTCTTTTGCACTGCATAATGATTCTCTAGATCATGACTGCTCAAGATTGGAATGTCTGGAGTTGGATGCTCCTTTGCCTTCATACATGCAAAAAAGTTGGGATATCATAAACAGTTAG

Coding sequence (CDS)

ATGGTAAATGTAGATGAAAATCATGAAACTTTTGAATTTGGGTTGAAGATGTTAGCATTGAAGTCTACTCCCAATTATGATGGGCATTGGGCGCCACCCACCATGGGACTCCGGGCACTCTTCCGTCCTTCCATCATTCTCCGTACTTCACCGTCTTGCACTGATCCCACCGGCGAAATTCTCTATTTCTCTATTTTTCATGTGCTGAAGTCCACTTTAGCCTTGAGTAACGCCTCCGAACCCGTATCCCAACAGAAGAGTGAGAAGGGAAGTAATAGATGGTTCACGCTGCCTCCTTACACTGCGACTGTAAACGGTTCGATTTTTGGTAATCGCATAATGAACGGAGGAGCTCAAGCCGAATTAGGAGCTTCCACCGCTGCAATAACTGCTCTGAAATGGGTGATTCGTTGCTGCCCGGAATTGCCGAGGAGCCTCGTGCAAAAGCTGTTTCGACTGAGAAAGGTTCGAAGAGGTTCTTCTATGATTGAAAATTCTGAATATAATTTGGAAGCGCGAGACCAACTTAAAAGAGTGGCAGCCAAGGACATTCTGAACGTAGGTGATCAAATTTTTCTCCCCATGTCAGTTCAAGTATTCTCCACAGAGAAAACTCGGGATTATTGCAATAAAGAGGAAGAGACATTCATGCATAGTCTAGTATTATACAAGGATCCAGCAATACTTGTTATCAATAAACCTCCTGGCTTGCCTGTACAGGGTGGTATTGGTGTCAAAAGAAGTTTAGATGAATTAGCTGCAAGTTGTTTAAGTTATGGCTACTCAGAACCACCTCGACTAGTACACAGGCTAGACAGGGACAGCAGTGGCATCTTGATAATGGGCAGGTCTTCCACAAGTACTTCACTTCTTCATTCTGTCTTCCGTGAGAAAACTTTTGAGGCATCAAAACATGCAAACCATGTTGATAGTGAGAAAAAGATTTTGCTAAAGAGGTATTGGGCACTTGTAATTGGTTCTCCTAGACGTTCAAAGGGGTTAATTACAGCTCCACTAGGAAAGATTGTCTTGGATGATCGAAGATCTGAGCAAATTATGGTCATTGATAATGCCGAACCTATGCCTTCTCAGCAAGCAATTACTCAATATAAAGTGATCAAATCTCATCATGGATACACTTGGTTGGAGTTGTGTCCCCTCACTGGAAGAAAACATCAGCTTCGTGTACACTGTGCCGAGGTATTAGGAACGCCTATAGTTGGAGACTACAAATATGGATGGCAAGCACATAAAAATTGGAAACCATTTCGTTCTAATTTCCACAAGAATCAAGGAAAGAAACAATTCCTCCCTTTTGGTCTTGACTTTGAAAGTGGAAGTATTGCAGAAAATAATCCTCGTCTACACCTTCATTGTAAGCAACTGACTTTACCAGATGTTTCTTTTGCACTGCATAATGATTCTCTAGATCATGACTGCTCAAGATTGGAATGTCTGGAGTTGGATGCTCCTTTGCCTTCATACATGCAAAAAAGTTGGGATATCATAAACAGTTAG

Protein sequence

MVNVDENHETFEFGLKMLALKSTPNYDGHWAPPTMGLRALFRPSIILRTSPSCTDPTGEILYFSIFHVLKSTLALSNASEPVSQQKSEKGSNRWFTLPPYTATVNGSIFGNRIMNGGAQAELGASTAAITALKWVIRCCPELPRSLVQKLFRLRKVRRGSSMIENSEYNLEARDQLKRVAAKDILNVGDQIFLPMSVQVFSTEKTRDYCNKEEETFMHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYGYSEPPRLVHRLDRDSSGILIMGRSSTSTSLLHSVFREKTFEASKHANHVDSEKKILLKRYWALVIGSPRRSKGLITAPLGKIVLDDRRSEQIMVIDNAEPMPSQQAITQYKVIKSHHGYTWLELCPLTGRKHQLRVHCAEVLGTPIVGDYKYGWQAHKNWKPFRSNFHKNQGKKQFLPFGLDFESGSIAENNPRLHLHCKQLTLPDVSFALHNDSLDHDCSRLECLELDAPLPSYMQKSWDIINS*
Homology
[Close]
BLAST of Chy1G000990 vs. ExPASy Swiss-Prot
Match: Q9LT72 (RNA pseudouridine synthase 4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At3g19440 PE=2 SV=1)

HSP 1 Score: 449.1 bits (1154), Expect = 6.5e-125
Identity = 233/441 (52.83%), Postives = 307/441 (69.61%), Query Frame = 0 

Query: 74  ALSNASEPVSQQKSEKGSNRWFTLPPYTATVNGSIFGNRIMNGGAQAELGASTAAITALK 133
           A   ++   S  +++    +W TLPP++ T++G+  G  +++ G   +     +  TAL+
Sbjct: 35  AAHQSTRSYSTTQTDDSRGKWLTLPPFSPTIDGTAVGKDLLSDGDSVKSSTDNSKTTALR 94

Query: 134 WVIRCCPELPRSLVQKLFRLRKVRRGSSM-IENSEYNLEARDQLKRVAAKDILNVGDQIF 193
           W++RC P+LPR+LVQKLFRLR+VRR  SM ++  E     R QLKRVAAK+ LNVGD+I+
Sbjct: 95  WILRCRPDLPRTLVQKLFRLRQVRREMSMSVDGDELQ---RSQLKRVAAKESLNVGDRIY 154

Query: 194 LPMSV-----QVFSTEKTRDYCNKEEETFMHSLVLYKDPAILVINKPPGLPVQGGIGVKR 253
           LP+SV     Q    +K    C+ EE  F+ SLVLYKDPAI+V+NKP GL VQGG G+K 
Sbjct: 155 LPLSVDNDTPQTPPAKKESFQCSDEERKFVCSLVLYKDPAIIVLNKPHGLAVQGGSGIKT 214

Query: 254 SLDELAASCLSYGYSEPPRLVHRLDRDSSGILIMGRSSTSTSLLHSVFREKTFEASKHAN 313
           S+DELAASCL +  SE PRLVHRLDRD SG+L++ R+ T+ ++LHS+FREKT  AS +  
Sbjct: 215 SIDELAASCLKFDKSESPRLVHRLDRDCSGLLVLARTQTAATVLHSIFREKTTGASAYG- 274

Query: 314 HVDSEKKILLKRYWALVIGSPRRSKGLITAPLGKIVLDDRRSEQIMVIDNAEPMPSQQAI 373
            V    K L ++Y ALVIG P R +G I+APL K+V+DD +SE+I V DN E + +Q AI
Sbjct: 275 -VKKNVKSLKRKYMALVIGCPPRQRGQISAPLRKVVVDDGKSERITVNDNGELVSTQHAI 334

Query: 374 TQYKVIKSH-HGYTWLELCPLTGRKHQLRVHCAEVLGTPIVGDYKYGWQAHKNWKPFRSN 433
           T+Y+VI+S  HGYTWLEL PLTGRKHQLRVHCAEVLGTPIVGDYKYGWQAHK  +PF S+
Sbjct: 335 TEYRVIESSPHGYTWLELRPLTGRKHQLRVHCAEVLGTPIVGDYKYGWQAHKAREPFVSS 394

Query: 434 FHKNQGKKQFLPFGLDFESGSIAENNPRLHLHCKQLTLPDVSFALH--NDSLDHDCSRLE 493
              N  K+   PFGLD + G ++   P LHLH KQ+ LP++S  L     S D D S L+
Sbjct: 395 -ENNPTKQSSSPFGLDLDGGDVSSKQPHLHLHSKQIDLPNISQLLEKMQVSSDSDISDLD 454

Query: 494 CLELDAPLPSYMQKSWDIINS 506
            L+ DAPLPS+MQ S++++ S
Sbjct: 455 SLKFDAPLPSHMQLSFNLLKS 469

[Close]
BLAST of Chy1G000990 vs. ExPASy Swiss-Prot
Match: Q5XET6 (RNA pseudouridine synthase 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g78910 PE=2 SV=1)

HSP 1 Score: 295.0 bits (754), Expect = 1.6e-78
Identity = 162/418 (38.76%), Postives = 244/418 (58.37%), Query Frame = 0 

Query: 115 NGGAQAELGASTAAITALKWVIRCCPEL---------PRSLVQKLFRLRKVRRGSSMIEN 174
           N GA  + G S   +TA+ WV     E+          + LVQ  FR R+        + 
Sbjct: 67  NAGADGDDGDSGGHVTAISWVKYYFEEIYDKAIQTHFTKGLVQMEFRGRR--------DA 126

Query: 175 SEYNLEARDQLKRVAAKDILNVGDQIFLPMSVQVFSTEKTRD-------YCNKEEETFMH 234
           S    +    ++++   +++ +GD+I+LP+S+      K  D       Y N +E  ++ 
Sbjct: 127 SREKEDGAIPMRKIKHNEVMQIGDKIWLPVSIAEMRISKRYDTIPSGTLYPNADEIAYLQ 186

Query: 235 SLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYGYSEPPRLVHRLDRDSSGI 294
            LV +KD AI+V+NKPP LPV+G + +  S+D LAA+ LS+G  E PRLVHRLDR++SG+
Sbjct: 187 RLVRFKDSAIIVLNKPPKLPVKGNVPIHNSMDALAAAALSFGNDEGPRLVHRLDRETSGL 246

Query: 295 LIMGRSSTSTSLLHSVFREKTFEASKHANHVDSEKKILLKRYWALVIGSPRRSKGLITAP 354
           L+MGR+  S   LHSVF +     S      +   + + ++YWALVIGSP+  +GLI+AP
Sbjct: 247 LVMGRTKESIDYLHSVFSDYKGRNSS-CKAWNKACEAMYQQYWALVIGSPKEKEGLISAP 306

Query: 355 LGKIVLDDRRSEQIMVIDNAEPMPSQQAITQYKVI-KSHHGYTWLELCPLTGRKHQLRVH 414
           L K++LDD +++++++   +    SQ AIT+YKV+    +G +W+EL P+T RKHQLRVH
Sbjct: 307 LSKVLLDDGKTDRVVLAQGSGFEASQDAITEYKVLGPKINGCSWVELRPITSRKHQLRVH 366

Query: 415 CAEVLGTPIVGDYKYGWQAHKNWKPF-RSNFHKNQGKKQFL--PFGLDFESGSIAENNPR 474
           CAE LGTPIVGDYKYGW  HK WK   + +     GK   L  P GLD + GS+    P 
Sbjct: 367 CAEALGTPIVGDYKYGWFVHKRWKQMPQVDIEPTTGKPYKLRRPEGLDVQKGSVLSKVPL 426

Query: 475 LHLHCKQLTLPDVSFALHNDSLDHD-------CSRLECLELDAPLPSYMQKSWDIINS 506
           LHLHC+++ LP+++  LH  +             + + L   A +PS+M+ SW++++S
Sbjct: 427 LHLHCREMVLPNIAKFLHVMNQQETEPLHTGIIDKPDLLRFVASMPSHMKISWNLMSS 475

[Close]
BLAST of Chy1G000990 vs. ExPASy Swiss-Prot
Match: Q0J4D4 (RNA pseudouridine synthase 3, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=Os08g0520100 PE=2 SV=1)

HSP 1 Score: 293.1 bits (749), Expect = 6.0e-78
Identity = 162/428 (37.85%), Postives = 256/428 (59.81%), Query Frame = 0 

Query: 96  TLPPYTATVNGSIFGNRIMNGGAQAELGASTAAITALKWVIRCCPELPRSLVQKLFRLRK 155
           +LPP+    +G     R           A+   +TA+ WV     ++P+ +VQ  F  R 
Sbjct: 55  SLPPFPGGGDGDPLPGR---------KAAAPRRVTAVSWVKHYLADVPQEVVQAHFNKRL 114

Query: 156 VRRGSSMIENSEYNLEA-RDQLKRVAAKDILNVGDQIFLPMSVQVFSTEKTRD------- 215
           V    S  E S   +++ +  LK++   D++  G +I LP+SV     +K  +       
Sbjct: 115 VYSECSDHEVSVETIKSQKHHLKKIKHNDVMEPGMRIHLPVSVAEGEIKKRYETIPTATL 174

Query: 216 YCNKEEETFMHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYGYSEPPRL 275
           + NK+E  ++  LV++KD AILV+NKPP +P++G + V  S+D LAA+ LSYG  E P+L
Sbjct: 175 HPNKDEIEYLRRLVIHKDSAILVLNKPPKVPMKGNLPVHNSMDVLAAAALSYGNEEGPKL 234

Query: 276 VHRLDRDSSGILIMGRSSTSTSLLHSVFREKTFEASKHANHVDSEKKILLKRYWALVIGS 335
           VHRLDR+SSG+L+ GR+  S + LH +F      A  ++   ++  +  ++RYWALVIG+
Sbjct: 235 VHRLDRESSGLLLFGRTKESFTRLHWLFTSVNL-AKTNSQVWNAACEAYMQRYWALVIGT 294

Query: 336 PRRSKGLITAPLGKIVLDDRRSEQIMVIDNAEPMPSQQAITQYKVI-KSHHGYTWLELCP 395
           P+  +G+I+APL K++LDD ++E++++   +    +Q+A+T Y+V+  + HG +W+EL P
Sbjct: 295 PKEREGIISAPLSKVLLDDGKAERVILAHPSGIDGAQEAVTAYRVMGPTIHGCSWIELRP 354

Query: 396 LTGRKHQLRVHCAEVLGTPIVGDYKYGWQAHKNWKPF-RSNFHKNQGKKQFL--PFGLDF 455
           LTGRKHQLRVHCAE LGTPIVGDYKYGW  H+ WK   + +F    G+   L  P GL+ 
Sbjct: 355 LTGRKHQLRVHCAEALGTPIVGDYKYGWFVHQRWKQNPQPDFEPFTGEPYKLRRPEGLEI 414

Query: 456 ESGSIAENNPRLHLHCKQLTLPDVSFAL------HNDSLDHDCSRLECLELDAPLPSYMQ 506
           + GS+    P LHLHC+++ +P+++  L      H +       +   L   AP+P++M+
Sbjct: 415 QKGSVLSKVPLLHLHCREMVIPNIAKFLSSNGEWHENGAPWSKEKPNLLRFIAPMPAHMK 472

[Close]
BLAST of Chy1G000990 vs. ExPASy Swiss-Prot
Match: Q69K07 (RNA pseudouridine synthase 4, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=Os09g0103500 PE=2 SV=1)

HSP 1 Score: 282.3 bits (721), Expect = 1.1e-74
Identity = 152/312 (48.72%), Postives = 210/312 (67.31%), Query Frame = 0 

Query: 85  QKSEKGSNRWFTLPPYTATVNGSIFGNRIMNGGAQAELGASTAAITALKWVIRCCPELPR 144
           +  E+G + W  LPP+ A ++ +     I  GG +   G      TA+KWV RCCP+LP 
Sbjct: 43  ETGERGKSPWLQLPPF-APLDAAAAARAISRGGGEG--GDGEQGATAIKWVRRCCPDLPT 102

Query: 145 SLVQKLFRLRKVRRGSSMIENSEYNLEA-RDQLKRVAAKDILNVGDQIFLPMSVQVFS-T 204
           SLVQKLFRLRKV++     E S  +  A + +L+RV+AKD L  GD +FLP++++  S  
Sbjct: 103 SLVQKLFRLRKVKKNVVTAEISSADASAEQHRLRRVSAKDQLMPGDILFLPVNLKESSVA 162

Query: 205 EKTRDYCNKEEETFMHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYGYS 264
           EKT+ + N+ E  F+  L +YKD AI+V+NKPPG+PVQGG+G+K S+D L AS      S
Sbjct: 163 EKTKKFDNRNEINFLRGLEIYKDEAIIVVNKPPGMPVQGGVGIKNSIDVL-ASMFEENSS 222

Query: 265 EPPRLVHRLDRDSSGILIMGRSSTSTSLLHSVFREKTFEASKHANHVDSEKKILLKRYWA 324
           E PRLVHRLDRD SGIL++GR+  STS+LH++FREKT +A       D  + +L ++Y A
Sbjct: 223 EAPRLVHRLDRDCSGILVLGRNQLSTSMLHAIFREKTADAL-----ADGTQHVLQRKYVA 282

Query: 325 LVIGSPRRSKGLITAPLGKIVLDDRRSEQIMVIDNAEPMPSQQAITQYKVIKS-HHGYTW 384
           LVIG+PR  KGL++APL KI+L D +SE++ +  ++     Q A+T+Y+VI+S   GYTW
Sbjct: 283 LVIGTPRHPKGLLSAPLAKILLQDGKSERLTIRASSNAASVQDALTEYRVIESCPQGYTW 342

Query: 385 LELCPLTGRKHQ 394
           LEL P TGRKHQ
Sbjct: 343 LELFPRTGRKHQ 345

[Close]
BLAST of Chy1G000990 vs. ExPASy Swiss-Prot
Match: Q9ZDR7 (Ribosomal large subunit pseudouridine synthase C OS=Rickettsia prowazekii (strain Madrid E) OX=272947 GN=rluC PE=3 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 9.6e-28
Identity = 90/282 (31.91%), Postives = 152/282 (53.90%), Query Frame = 0 

Query: 133 KWVIRCCPELPRSLVQKLFRLRKVRRGSSMIENSEYNLEARDQLKRVAAKDILNVGDQIF 192
           K++ R  P L + +++K  R +++   +   + +E NL       RV   D + + D   
Sbjct: 15  KYLKRLYPLLTQGVIEKALRQKQI---TVNFQKAEANL-------RVKVGDTIFINDYFN 74

Query: 193 LPMSVQVFSTEKTRDYCNKEEETFMHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDEL 252
           LP  V    T    D   K  +  +   ++Y+D  ++ INKP  L  QGG  +  S+D  
Sbjct: 75  LP--VTQHETLVFADAEIKLAKKILTDYLIYEDDHLIAINKPASLATQGGSKINLSIDS- 134

Query: 253 AASCLSYGYSEPPRLVHRLDRDSSGILIMGRSSTSTSLLHSVFREKTFEASKHANHVDSE 312
           A   L+Y  ++  +LVHRLD+++SG+L++ ++  S+  LH+ F+E               
Sbjct: 135 ALKYLNYQGAD-FKLVHRLDKETSGLLLIAKNYLSSVKLHNAFKE--------------- 194

Query: 313 KKILLKRYWALVIGSPRRSKGLITAPLGKIVLDDRRSEQIMVIDNAEPMPSQQAITQYKV 372
            K+++K+Y+A+  G P ++ G + + +GK     RR  +I  ID+ +    + AIT YK+
Sbjct: 195 -KLVIKKYFAITYGRPVKNVGTVKSNIGK---SKRRLSKIANIDSDD---GKLAITYYKL 254

Query: 373 IKSHHGYTWL-ELCPLTGRKHQLRVHCAEVLGTPIVGDYKYG 414
           +KS +   +L E  P+TGR HQLR+H A++LG PI+GD KYG
Sbjct: 255 LKSLNNNLFLIEFMPVTGRMHQLRLH-AQLLGCPILGDDKYG 259

[Close]
BLAST of Chy1G000990 vs. ExPASy TrEMBL
Match: A0A5D3C4F5 (RNA pseudouridine synthase 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G00930 PE=4 SV=1)

HSP 1 Score: 906.0 bits (2340), Expect = 7.2e-260
Identity = 442/471 (93.84%), Postives = 453/471 (96.18%), Query Frame = 0 

Query: 35  MGLRALFRPSIILRTSPSCTDPTGEILYFSIFHVLKSTLALSNASEPVSQQKSEKGSNRW 94
           M L  LFRPS+ILRTSPS TDP+ EILYFSIFHVLKSTLALSNASEPVSQQK+EKGSNRW
Sbjct: 1   MVLPPLFRPSVILRTSPSYTDPSSEILYFSIFHVLKSTLALSNASEPVSQQKNEKGSNRW 60

Query: 95  FTLPPYTATVNGSIFGNRIMNGGAQAELGASTAAITALKWVIRCCPELPRSLVQKLFRLR 154
           FTLPPYTATVN SI GNRI+N GAQAE+  STAAITALKWVI CCPELPRSLVQKLFRLR
Sbjct: 61  FTLPPYTATVNASILGNRILNRGAQAEVETSTAAITALKWVIHCCPELPRSLVQKLFRLR 120

Query: 155 KVRRGSSMIENSEYNLEARDQLKRVAAKDILNVGDQIFLPMSVQVFSTEKTRDYCNKEEE 214
           KVRRGSSMIENSEYNLEARDQLKRVAAKDILNVGDQIFLP+S QVFSTEKTRDYCNK+EE
Sbjct: 121 KVRRGSSMIENSEYNLEARDQLKRVAAKDILNVGDQIFLPISAQVFSTEKTRDYCNKDEE 180

Query: 215 TFMHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYGYSEPPRLVHRLDRD 274
           TFMHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYGYSEPPRLVHRLDRD
Sbjct: 181 TFMHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYGYSEPPRLVHRLDRD 240

Query: 275 SSGILIMGRSSTSTSLLHSVFREKTFEASKHANHVDSEKKILLKRYWALVIGSPRRSKGL 334
           SSGILIMGR+STSTSLLHSVFREKTF+ASKHANHVDSEKKILLKRYWALVIGSPRRSKGL
Sbjct: 241 SSGILIMGRTSTSTSLLHSVFREKTFDASKHANHVDSEKKILLKRYWALVIGSPRRSKGL 300

Query: 335 ITAPLGKIVLDDRRSEQIMVIDNAEPMPSQQAITQYKVIKSHHGYTWLELCPLTGRKHQL 394
           ITAPLGKIVLDD RSE+IMVIDNAEPMP QQAIT+YKVIKSHHGYTWLELCPLTGRKHQL
Sbjct: 301 ITAPLGKIVLDDGRSERIMVIDNAEPMPFQQAITRYKVIKSHHGYTWLELCPLTGRKHQL 360

Query: 395 RVHCAEVLGTPIVGDYKYGWQAHKNWKPFRSNFHKNQGKKQFLPFGLDFESGSIAENNPR 454
           RVHCAEVLGTPIVGDYKYGWQAHKNWKPF  NFHKNQGKKQFLPFGLDFESGSIAE NPR
Sbjct: 361 RVHCAEVLGTPIVGDYKYGWQAHKNWKPFHYNFHKNQGKKQFLPFGLDFESGSIAETNPR 420

Query: 455 LHLHCKQLTLPDVSFALHNDSLDHDCSRLECLELDAPLPSYMQKSWDIINS 506
           LHLHCKQLTLPDVSFALHNDSLD DCSRLECLELDAPLPSYMQKSWDIINS
Sbjct: 421 LHLHCKQLTLPDVSFALHNDSLDQDCSRLECLELDAPLPSYMQKSWDIINS 471

[Close]
BLAST of Chy1G000990 vs. ExPASy TrEMBL
Match: A0A1S3BZE7 (RNA pseudouridine synthase 4, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103495275 PE=4 SV=1)

HSP 1 Score: 906.0 bits (2340), Expect = 7.2e-260
Identity = 442/471 (93.84%), Postives = 453/471 (96.18%), Query Frame = 0 

Query: 35  MGLRALFRPSIILRTSPSCTDPTGEILYFSIFHVLKSTLALSNASEPVSQQKSEKGSNRW 94
           M L  LFRPS+ILRTSPS TDP+ EILYFSIFHVLKSTLALSNASEPVSQQK+EKGSNRW
Sbjct: 1   MVLPPLFRPSVILRTSPSYTDPSSEILYFSIFHVLKSTLALSNASEPVSQQKNEKGSNRW 60

Query: 95  FTLPPYTATVNGSIFGNRIMNGGAQAELGASTAAITALKWVIRCCPELPRSLVQKLFRLR 154
           FTLPPYTATVN SI GNRI+N GAQAE+  STAAITALKWVI CCPELPRSLVQKLFRLR
Sbjct: 61  FTLPPYTATVNASILGNRILNRGAQAEVETSTAAITALKWVIHCCPELPRSLVQKLFRLR 120

Query: 155 KVRRGSSMIENSEYNLEARDQLKRVAAKDILNVGDQIFLPMSVQVFSTEKTRDYCNKEEE 214
           KVRRGSSMIENSEYNLEARDQLKRVAAKDILNVGDQIFLP+S QVFSTEKTRDYCNK+EE
Sbjct: 121 KVRRGSSMIENSEYNLEARDQLKRVAAKDILNVGDQIFLPISAQVFSTEKTRDYCNKDEE 180

Query: 215 TFMHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYGYSEPPRLVHRLDRD 274
           TFMHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYGYSEPPRLVHRLDRD
Sbjct: 181 TFMHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYGYSEPPRLVHRLDRD 240

Query: 275 SSGILIMGRSSTSTSLLHSVFREKTFEASKHANHVDSEKKILLKRYWALVIGSPRRSKGL 334
           SSGILIMGR+STSTSLLHSVFREKTF+ASKHANHVDSEKKILLKRYWALVIGSPRRSKGL
Sbjct: 241 SSGILIMGRTSTSTSLLHSVFREKTFDASKHANHVDSEKKILLKRYWALVIGSPRRSKGL 300

Query: 335 ITAPLGKIVLDDRRSEQIMVIDNAEPMPSQQAITQYKVIKSHHGYTWLELCPLTGRKHQL 394
           ITAPLGKIVLDD RSE+IMVIDNAEPMP QQAIT+YKVIKSHHGYTWLELCPLTGRKHQL
Sbjct: 301 ITAPLGKIVLDDGRSERIMVIDNAEPMPFQQAITRYKVIKSHHGYTWLELCPLTGRKHQL 360

Query: 395 RVHCAEVLGTPIVGDYKYGWQAHKNWKPFRSNFHKNQGKKQFLPFGLDFESGSIAENNPR 454
           RVHCAEVLGTPIVGDYKYGWQAHKNWKPF  NFHKNQGKKQFLPFGLDFESGSIAE NPR
Sbjct: 361 RVHCAEVLGTPIVGDYKYGWQAHKNWKPFHYNFHKNQGKKQFLPFGLDFESGSIAETNPR 420

Query: 455 LHLHCKQLTLPDVSFALHNDSLDHDCSRLECLELDAPLPSYMQKSWDIINS 506
           LHLHCKQLTLPDVSFALHNDSLD DCSRLECLELDAPLPSYMQKSWDIINS
Sbjct: 421 LHLHCKQLTLPDVSFALHNDSLDQDCSRLECLELDAPLPSYMQKSWDIINS 471

[Close]
BLAST of Chy1G000990 vs. ExPASy TrEMBL
Match: A0A6J1GMP9 (RNA pseudouridine synthase 4, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111455375 PE=4 SV=1)

HSP 1 Score: 804.7 bits (2077), Expect = 2.2e-229
Identity = 396/471 (84.08%), Postives = 421/471 (89.38%), Query Frame = 0 

Query: 35  MGLRALFRPSIILRTSPSCTDPTGEILYFSIFHVLKSTLALSNASEPVSQQKSEKGSNRW 94
           M L A+ R S+I R+ PS   P GE L+FSIF   KST ALS A+E VS+QK+EKGSNRW
Sbjct: 1   MVLPAVLRRSVIRRSFPSSAAPGGESLHFSIFCERKSTYALSIATETVSEQKNEKGSNRW 60

Query: 95  FTLPPYTATVNGSIFGNRIMNGGAQAELGASTAAITALKWVIRCCPELPRSLVQKLFRLR 154
           FTLPP+TAT NGS  GNRIMNGGAQAE   ST AITALKWV RCCPELPRSLVQKLFRLR
Sbjct: 61  FTLPPFTATANGSALGNRIMNGGAQAEAETSTTAITALKWVFRCCPELPRSLVQKLFRLR 120

Query: 155 KVRRGSSMIENSEYNLEARDQLKRVAAKDILNVGDQIFLPMSVQVFSTEKTRDYCNKEEE 214
           KVRRGSSMIENSE NLEARDQLKRVAA DILN+GD++ LP+SVQVF TE+  D CNKEE+
Sbjct: 121 KVRRGSSMIENSECNLEARDQLKRVAANDILNIGDKLCLPISVQVFPTERAPDCCNKEEK 180

Query: 215 TFMHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYGYSEPPRLVHRLDRD 274
            F+HSLVLYKDPAIL INKPPGLPVQGGIGVKRSLD+LAASCLSY YSEPPRLVHRLDRD
Sbjct: 181 KFIHSLVLYKDPAILAINKPPGLPVQGGIGVKRSLDQLAASCLSYDYSEPPRLVHRLDRD 240

Query: 275 SSGILIMGRSSTSTSLLHSVFREKTFEASKHANHVDSEKKILLKRYWALVIGSPRRSKGL 334
           SSGILIMGR+STSTS+LHS+FREKT  ASKHANHVDSEKKIL KRYWALVIGSPRR+KGL
Sbjct: 241 SSGILIMGRTSTSTSVLHSIFREKTLGASKHANHVDSEKKILQKRYWALVIGSPRRTKGL 300

Query: 335 ITAPLGKIVLDDRRSEQIMVIDNAEPMPSQQAITQYKVIKSHHGYTWLELCPLTGRKHQL 394
           ITAPL K+VLDD RSEQIMVIDNAE MP Q AIT YKVIKSHHGYTWLELCPLTGRKHQL
Sbjct: 301 ITAPLVKMVLDDGRSEQIMVIDNAESMPFQHAITTYKVIKSHHGYTWLELCPLTGRKHQL 360

Query: 395 RVHCAEVLGTPIVGDYKYGWQAHKNWKPFRSNFHKNQGKKQFLPFGLDFESGSIAENNPR 454
           RVHCAEVLGTPIVGDYKYGWQAH+NWKPF  N HKNQGKKQ LPFGLDFESGSIAEN+PR
Sbjct: 361 RVHCAEVLGTPIVGDYKYGWQAHRNWKPFPHNSHKNQGKKQSLPFGLDFESGSIAENSPR 420

Query: 455 LHLHCKQLTLPDVSFALHNDSLDHDCSRLECLELDAPLPSYMQKSWDIINS 506
           LHLHCKQLTLPDVS ALHNDSLDHDCSRLE LELDAPLPSYMQ+SWDIIN+
Sbjct: 421 LHLHCKQLTLPDVSCALHNDSLDHDCSRLERLELDAPLPSYMQRSWDIINN 471

[Close]
BLAST of Chy1G000990 vs. ExPASy TrEMBL
Match: A0A6J1IBC5 (RNA pseudouridine synthase 4, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111470950 PE=4 SV=1)

HSP 1 Score: 790.0 bits (2039), Expect = 5.7e-225
Identity = 391/472 (82.84%), Postives = 416/472 (88.14%), Query Frame = 0 

Query: 35  MGLRALFRPSIILRTSPSCTDPTGEILYFSIFHVLKSTLALSNASEPVSQQKSEKGSNRW 94
           M L AL R S+I R+ PS   P GE L+FSIF   KST ALSNA+E VS+QK+EKGSNRW
Sbjct: 1   MVLPALLRRSVIRRSFPSSAAPGGESLHFSIFCERKSTFALSNATETVSEQKNEKGSNRW 60

Query: 95  FTLPPYTATVNGSIFGNRIMNGGAQAELGASTAAITALKWVIRCCPELPRSLVQKLFRLR 154
           FTLPP+TATVNGS  GNRIMNGGAQ+E   ST AITALKWV RCCPELPRSLVQKLFRLR
Sbjct: 61  FTLPPFTATVNGSALGNRIMNGGAQSEAETSTTAITALKWVFRCCPELPRSLVQKLFRLR 120

Query: 155 KVRRGSSMIENSEYNLEARDQLKRVAAKDILNVGDQIFLPMSVQVFSTEKTRDYCNKEEE 214
           KVRRGSSMIENSE NLEARDQLKRVAA DILN+GD++ LP+SVQVF TE+  D CNKEE+
Sbjct: 121 KVRRGSSMIENSECNLEARDQLKRVAANDILNIGDKLCLPISVQVFPTERAPDCCNKEEK 180

Query: 215 TFMHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYGYSEPPRLVHRLDRD 274
            F+HSLVLYKDPAIL INKPPGLPVQGGIGVKRSLDELAASCLSY YSEPPRLVHRLDRD
Sbjct: 181 KFIHSLVLYKDPAILAINKPPGLPVQGGIGVKRSLDELAASCLSYDYSEPPRLVHRLDRD 240

Query: 275 SSGILIMGRSSTSTSLLHSVFREKTFEASKHANHVDSEKKILLKRYWALVIGSPRRSKGL 334
           SSGILIMGR+STSTS+LHS+FREKT  ASKHANHVD EK IL KRYWALVIGSP  +KGL
Sbjct: 241 SSGILIMGRTSTSTSVLHSIFREKTLGASKHANHVDREKMILQKRYWALVIGSPSHTKGL 300

Query: 335 ITAPLGKIVLDDRRSEQIMVIDNAEP-MPSQQAITQYKVIKSHHGYTWLELCPLTGRKHQ 394
           ITAPL K+VLDD RSEQIMV+DNAE  MP Q AIT YKVIKSHHGYTW+ELCPLTGRKHQ
Sbjct: 301 ITAPLVKMVLDDGRSEQIMVVDNAESIMPFQHAITTYKVIKSHHGYTWVELCPLTGRKHQ 360

Query: 395 LRVHCAEVLGTPIVGDYKYGWQAHKNWKPFRSNFHKNQGKKQFLPFGLDFESGSIAENNP 454
           LRVHCAE LGTPIVGDYKYGWQAH+N KPF  N HKNQGKKQ LPFGLDFESGSIAENNP
Sbjct: 361 LRVHCAEALGTPIVGDYKYGWQAHRNLKPFPHNSHKNQGKKQSLPFGLDFESGSIAENNP 420

Query: 455 RLHLHCKQLTLPDVSFALHNDSLDHDCSRLECLELDAPLPSYMQKSWDIINS 506
           RLHLHCK LTLPDVS ALHNDSLDHDCSRLE LELDAPLPSYMQ+SWDIIN+
Sbjct: 421 RLHLHCKHLTLPDVSCALHNDSLDHDCSRLERLELDAPLPSYMQRSWDIINN 472

[Close]
BLAST of Chy1G000990 vs. ExPASy TrEMBL
Match: A0A6J1D432 (RNA pseudouridine synthase 4, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111017405 PE=4 SV=1)

HSP 1 Score: 788.5 bits (2035), Expect = 1.7e-224
Identity = 386/472 (81.78%), Postives = 423/472 (89.62%), Query Frame = 0 

Query: 35  MGLRALFRPSIILRTSPSCTDPTGEILYFSIFHVLKSTLALSNASEPVSQQKSE-KGSNR 94
           M L AL R S+IL  +PS T   G  L+FS+FH LKST ALSN SE +S++K+E KG NR
Sbjct: 1   MLLPALLRRSLILSGTPSSTARGGHSLHFSVFHELKSTFALSNTSETISEEKNEKKGFNR 60

Query: 95  WFTLPPYTATVNGSIFGNRIMNGGAQAELGASTAAITALKWVIRCCPELPRSLVQKLFRL 154
           WFTLPPYTATV+GS+ GNRI+N G+QAE   ST AITALKWV+RCCPELPRSLVQKLFRL
Sbjct: 61  WFTLPPYTATVDGSVLGNRILNQGSQAEDETSTTAITALKWVLRCCPELPRSLVQKLFRL 120

Query: 155 RKVRRGSSMIENSEYNLEARDQLKRVAAKDILNVGDQIFLPMSVQVFSTEKTRDYCNKEE 214
           RK+RRGSSMIE+SE NLEA++QLKRVAAKDILNVGDQIFLP+SVQVF  E+ RDYCNKEE
Sbjct: 121 RKIRRGSSMIESSECNLEAQNQLKRVAAKDILNVGDQIFLPISVQVFPRERARDYCNKEE 180

Query: 215 ETFMHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYGYSEPPRLVHRLDR 274
           E FMHSLVLYKDPAI+VINKPPGLPVQGGIG+ RSLDELAASCLSY YSEPPRLVHRLDR
Sbjct: 181 EKFMHSLVLYKDPAIIVINKPPGLPVQGGIGITRSLDELAASCLSYDYSEPPRLVHRLDR 240

Query: 275 DSSGILIMGRSSTSTSLLHSVFREKTFEASKHANHVDSEKKILLKRYWALVIGSPRRSKG 334
           DSSGIL+MGR+STSTS+LHS+FREKTF ASKH  HVDSEKKIL K+YWALVIGSPRR  G
Sbjct: 241 DSSGILVMGRTSTSTSVLHSIFREKTFGASKH--HVDSEKKILQKKYWALVIGSPRRPNG 300

Query: 335 LITAPLGKIVLDDRRSEQIMVIDNAEPMPSQQAITQYKVIKSHHGYTWLELCPLTGRKHQ 394
           LITAPLGKIVLDD RSE+IMVIDNAE M  QQAIT Y+VIKSHHGYTWLELCPLTGRKHQ
Sbjct: 301 LITAPLGKIVLDDGRSEKIMVIDNAESMAFQQAITTYRVIKSHHGYTWLELCPLTGRKHQ 360

Query: 395 LRVHCAEVLGTPIVGDYKYGWQAHKNWKPFRSNFHKNQGKKQFLPFGLDFESGSIAENNP 454
           LRVHCAEVLGTPIVGDYKYGWQAH+NW+PF SNFHKNQGKKQ LPFGLDFE+GS+AEN+P
Sbjct: 361 LRVHCAEVLGTPIVGDYKYGWQAHRNWEPFPSNFHKNQGKKQSLPFGLDFENGSVAENSP 420

Query: 455 RLHLHCKQLTLPDVSFALHNDSLDHDCSRLECLELDAPLPSYMQKSWDIINS 506
           RLHLHCKQL LP+VS AL NDSLDHD S LECLELDAPLPSYM++SWDIIN+
Sbjct: 421 RLHLHCKQLALPNVSGALPNDSLDHDFSELECLELDAPLPSYMRRSWDIINN 470

[Close]
BLAST of Chy1G000990 vs. NCBI nr
Match: XP_011658889.1 (RNA pseudouridine synthase 4, mitochondrial [Cucumis sativus] >KGN43951.2 hypothetical protein Csa_017092 [Cucumis sativus])

HSP 1 Score: 940 bits (2429), Expect = 0.0
Identity = 460/471 (97.66%), Postives = 465/471 (98.73%), Query Frame = 0 

Query: 35  MGLRALFRPSIILRTSPSCTDPTGEILYFSIFHVLKSTLALSNASEPVSQQKSEKGSNRW 94
           MGLRALFRPS+ILRTSPSCTDP+GEILYFSIFHVLKSTLALSNASEPVSQQK+EKGSNRW
Sbjct: 1   MGLRALFRPSVILRTSPSCTDPSGEILYFSIFHVLKSTLALSNASEPVSQQKNEKGSNRW 60

Query: 95  FTLPPYTATVNGSIFGNRIMNGGAQAELGASTAAITALKWVIRCCPELPRSLVQKLFRLR 154
           FTLPPYTATVNGSI GNRIMNGG QAELGASTAAITALKWVIRCCPELPRSLVQKLFRLR
Sbjct: 61  FTLPPYTATVNGSILGNRIMNGGGQAELGASTAAITALKWVIRCCPELPRSLVQKLFRLR 120

Query: 155 KVRRGSSMIENSEYNLEARDQLKRVAAKDILNVGDQIFLPMSVQVFSTEKTRDYCNKEEE 214
           KVRRGSSMIENSEYNLEARDQLKRVAAKDILNVGDQIFLPMSVQVFSTEKTRDYCNKEEE
Sbjct: 121 KVRRGSSMIENSEYNLEARDQLKRVAAKDILNVGDQIFLPMSVQVFSTEKTRDYCNKEEE 180

Query: 215 TFMHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYGYSEPPRLVHRLDRD 274
           TFMHSLVL+KDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYGYSEPPRLVHRLDRD
Sbjct: 181 TFMHSLVLHKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYGYSEPPRLVHRLDRD 240

Query: 275 SSGILIMGRSSTSTSLLHSVFREKTFEASKHANHVDSEKKILLKRYWALVIGSPRRSKGL 334
           SSGILIMGR+STSTSLLHSVFREKTFEASKHANHVDSEKKILLKRYWALVIGSPRRSKGL
Sbjct: 241 SSGILIMGRTSTSTSLLHSVFREKTFEASKHANHVDSEKKILLKRYWALVIGSPRRSKGL 300

Query: 335 ITAPLGKIVLDDRRSEQIMVIDNAEPMPSQQAITQYKVIKSHHGYTWLELCPLTGRKHQL 394
           ITAPLGKIVLDD RSEQIMVIDNAEPMP QQAITQYKVIKSHHGYTWLELCPLTGRKHQL
Sbjct: 301 ITAPLGKIVLDDGRSEQIMVIDNAEPMPFQQAITQYKVIKSHHGYTWLELCPLTGRKHQL 360

Query: 395 RVHCAEVLGTPIVGDYKYGWQAHKNWKPFRSNFHKNQGKKQFLPFGLDFESGSIAENNPR 454
           RVHCAEVLGTPIVGDYKYGWQAHKNWKPF SNFHKNQGKKQFLPFGLDFESGSIAENNPR
Sbjct: 361 RVHCAEVLGTPIVGDYKYGWQAHKNWKPFCSNFHKNQGKKQFLPFGLDFESGSIAENNPR 420

Query: 455 LHLHCKQLTLPDVSFALHNDSLDHDCSRLECLELDAPLPSYMQKSWDIINS 505
           LHLHCKQLTLPDVSFALHNDSLD DCSRLECLELDAPLPSYMQKSWDIINS
Sbjct: 421 LHLHCKQLTLPDVSFALHNDSLDRDCSRLECLELDAPLPSYMQKSWDIINS 471

[Close]
BLAST of Chy1G000990 vs. NCBI nr
Match: XP_008454996.1 (PREDICTED: RNA pseudouridine synthase 4, mitochondrial [Cucumis melo] >KAA0031338.1 RNA pseudouridine synthase 4 [Cucumis melo var. makuwa] >TYK06791.1 RNA pseudouridine synthase 4 [Cucumis melo var. makuwa])

HSP 1 Score: 902 bits (2332), Expect = 0.0
Identity = 442/471 (93.84%), Postives = 453/471 (96.18%), Query Frame = 0 

Query: 35  MGLRALFRPSIILRTSPSCTDPTGEILYFSIFHVLKSTLALSNASEPVSQQKSEKGSNRW 94
           M L  LFRPS+ILRTSPS TDP+ EILYFSIFHVLKSTLALSNASEPVSQQK+EKGSNRW
Sbjct: 1   MVLPPLFRPSVILRTSPSYTDPSSEILYFSIFHVLKSTLALSNASEPVSQQKNEKGSNRW 60

Query: 95  FTLPPYTATVNGSIFGNRIMNGGAQAELGASTAAITALKWVIRCCPELPRSLVQKLFRLR 154
           FTLPPYTATVN SI GNRI+N GAQAE+  STAAITALKWVI CCPELPRSLVQKLFRLR
Sbjct: 61  FTLPPYTATVNASILGNRILNRGAQAEVETSTAAITALKWVIHCCPELPRSLVQKLFRLR 120

Query: 155 KVRRGSSMIENSEYNLEARDQLKRVAAKDILNVGDQIFLPMSVQVFSTEKTRDYCNKEEE 214
           KVRRGSSMIENSEYNLEARDQLKRVAAKDILNVGDQIFLP+S QVFSTEKTRDYCNK+EE
Sbjct: 121 KVRRGSSMIENSEYNLEARDQLKRVAAKDILNVGDQIFLPISAQVFSTEKTRDYCNKDEE 180

Query: 215 TFMHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYGYSEPPRLVHRLDRD 274
           TFMHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYGYSEPPRLVHRLDRD
Sbjct: 181 TFMHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYGYSEPPRLVHRLDRD 240

Query: 275 SSGILIMGRSSTSTSLLHSVFREKTFEASKHANHVDSEKKILLKRYWALVIGSPRRSKGL 334
           SSGILIMGR+STSTSLLHSVFREKTF+ASKHANHVDSEKKILLKRYWALVIGSPRRSKGL
Sbjct: 241 SSGILIMGRTSTSTSLLHSVFREKTFDASKHANHVDSEKKILLKRYWALVIGSPRRSKGL 300

Query: 335 ITAPLGKIVLDDRRSEQIMVIDNAEPMPSQQAITQYKVIKSHHGYTWLELCPLTGRKHQL 394
           ITAPLGKIVLDD RSE+IMVIDNAEPMP QQAIT+YKVIKSHHGYTWLELCPLTGRKHQL
Sbjct: 301 ITAPLGKIVLDDGRSERIMVIDNAEPMPFQQAITRYKVIKSHHGYTWLELCPLTGRKHQL 360

Query: 395 RVHCAEVLGTPIVGDYKYGWQAHKNWKPFRSNFHKNQGKKQFLPFGLDFESGSIAENNPR 454
           RVHCAEVLGTPIVGDYKYGWQAHKNWKPF  NFHKNQGKKQFLPFGLDFESGSIAE NPR
Sbjct: 361 RVHCAEVLGTPIVGDYKYGWQAHKNWKPFHYNFHKNQGKKQFLPFGLDFESGSIAETNPR 420

Query: 455 LHLHCKQLTLPDVSFALHNDSLDHDCSRLECLELDAPLPSYMQKSWDIINS 505
           LHLHCKQLTLPDVSFALHNDSLD DCSRLECLELDAPLPSYMQKSWDIINS
Sbjct: 421 LHLHCKQLTLPDVSFALHNDSLDQDCSRLECLELDAPLPSYMQKSWDIINS 471

[Close]
BLAST of Chy1G000990 vs. NCBI nr
Match: XP_038888757.1 (RNA pseudouridine synthase 4, mitochondrial [Benincasa hispida])

HSP 1 Score: 837 bits (2162), Expect = 9.71e-304
Identity = 411/470 (87.45%), Postives = 429/470 (91.28%), Query Frame = 0 

Query: 35  MGLRALFRPSIILRTSPSCTDPTGEILYFSIFHVLKSTLALSNASEPVSQQKSEKGSNRW 94
           M L AL R S+ILR   S   P GE  +FSIFH LKSTLALSN +E VS +K+EKGSNRW
Sbjct: 1   MVLPALIRRSVILRIPLSSAAPVGESFHFSIFHELKSTLALSNTTETVSHRKNEKGSNRW 60

Query: 95  FTLPPYTATVNGSIFGNRIMNGGAQAELGASTAAITALKWVIRCCPELPRSLVQKLFRLR 154
           FTLPPYTATVNGS+ GNRI+  G QAE   STAAITALKWVIRCCPELPRSLVQKLFRLR
Sbjct: 61  FTLPPYTATVNGSLLGNRILKRGDQAEAEISTAAITALKWVIRCCPELPRSLVQKLFRLR 120

Query: 155 KVRRGSSMIENSEYNLEARDQLKRVAAKDILNVGDQIFLPMSVQVFSTEKTRDYCNKEEE 214
           KVRRGSSMI NSE NLEARDQLKRVAAKDILN+GDQIFLP+SVQVF TEK RDYCNKEEE
Sbjct: 121 KVRRGSSMIGNSECNLEARDQLKRVAAKDILNIGDQIFLPISVQVFPTEKARDYCNKEEE 180

Query: 215 TFMHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYGYSEPPRLVHRLDRD 274
            F+HSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSY YSEPPRLVHRLDRD
Sbjct: 181 KFIHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYDYSEPPRLVHRLDRD 240

Query: 275 SSGILIMGRSSTSTSLLHSVFREKTFEASKHANHVDSEKKILLKRYWALVIGSPRRSKGL 334
           SSGILIMGR+STSTSLLHSVFREKTFEASKHANHVDSEKKIL KRYWALVIGSPRRSKGL
Sbjct: 241 SSGILIMGRTSTSTSLLHSVFREKTFEASKHANHVDSEKKILQKRYWALVIGSPRRSKGL 300

Query: 335 ITAPLGKIVLDDRRSEQIMVIDNAEPMPSQQAITQYKVIKSHHGYTWLELCPLTGRKHQL 394
           ITAPLGK V+DD RSEQIMVIDNAE MP QQAIT+YKVIKSHHGYTWLELCP+TGRKHQL
Sbjct: 301 ITAPLGKFVMDDGRSEQIMVIDNAESMPFQQAITRYKVIKSHHGYTWLELCPVTGRKHQL 360

Query: 395 RVHCAEVLGTPIVGDYKYGWQAHKNWKPFRSNFHKNQGKKQFLPFGLDFESGSIAENNPR 454
           RVHCAEVLGTPIVGDYKYGWQAH+NWKPF SNFHKN+GKKQ  PFGLDFESGSIAENNPR
Sbjct: 361 RVHCAEVLGTPIVGDYKYGWQAHRNWKPFHSNFHKNEGKKQSFPFGLDFESGSIAENNPR 420

Query: 455 LHLHCKQLTLPDVSFALHNDSLDHDCSRLECLELDAPLPSYMQKSWDIIN 504
           LHLHCKQLTLPDVSFALHNDS+D  CSRLECLELDAPLP YMQ+SWDIIN
Sbjct: 421 LHLHCKQLTLPDVSFALHNDSIDQKCSRLECLELDAPLPLYMQRSWDIIN 470

[Close]
BLAST of Chy1G000990 vs. NCBI nr
Match: XP_023553811.1 (RNA pseudouridine synthase 4, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 804 bits (2077), Expect = 8.55e-291
Identity = 396/471 (84.08%), Postives = 421/471 (89.38%), Query Frame = 0 

Query: 35  MGLRALFRPSIILRTSPSCTDPTGEILYFSIFHVLKSTLALSNASEPVSQQKSEKGSNRW 94
           M L A+ R S+I R+ PS   P GE L+FSIF   KST ALSNA+E VS+QK+EKGSNRW
Sbjct: 1   MVLPAVLRRSVIRRSFPSSAAPGGESLHFSIFCERKSTFALSNATETVSEQKNEKGSNRW 60

Query: 95  FTLPPYTATVNGSIFGNRIMNGGAQAELGASTAAITALKWVIRCCPELPRSLVQKLFRLR 154
           FTLPP+TATVNGS  GNRIMNGGAQAE   ST AITALKWV RCCPELPRSLVQKLFRLR
Sbjct: 61  FTLPPFTATVNGSALGNRIMNGGAQAEAETSTTAITALKWVFRCCPELPRSLVQKLFRLR 120

Query: 155 KVRRGSSMIENSEYNLEARDQLKRVAAKDILNVGDQIFLPMSVQVFSTEKTRDYCNKEEE 214
           KVRRGSSMIENSE NLEARDQLKRVAA DILN+GD++ LP+SVQVF TE+  D CNKEE+
Sbjct: 121 KVRRGSSMIENSECNLEARDQLKRVAANDILNIGDKLCLPISVQVFPTERAPDCCNKEEK 180

Query: 215 TFMHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYGYSEPPRLVHRLDRD 274
            F+HSLVLYKDPAIL INKPPGLPVQGGIGVKRSLDELAASCLSY YSEPPRLVHRLDRD
Sbjct: 181 KFIHSLVLYKDPAILAINKPPGLPVQGGIGVKRSLDELAASCLSYDYSEPPRLVHRLDRD 240

Query: 275 SSGILIMGRSSTSTSLLHSVFREKTFEASKHANHVDSEKKILLKRYWALVIGSPRRSKGL 334
           SSGILIMGR+STSTS+LHS+FREKT  ASKHANHVD EKKIL KRYWALVIGSPRR+KGL
Sbjct: 241 SSGILIMGRTSTSTSVLHSIFREKTLGASKHANHVDREKKILQKRYWALVIGSPRRTKGL 300

Query: 335 ITAPLGKIVLDDRRSEQIMVIDNAEPMPSQQAITQYKVIKSHHGYTWLELCPLTGRKHQL 394
           ITAPL K+VLDD RSEQIMV+DNAE MP Q AIT YKVIK+HHGYTWLELCPLTGRKHQL
Sbjct: 301 ITAPLVKMVLDDGRSEQIMVVDNAESMPFQHAITTYKVIKTHHGYTWLELCPLTGRKHQL 360

Query: 395 RVHCAEVLGTPIVGDYKYGWQAHKNWKPFRSNFHKNQGKKQFLPFGLDFESGSIAENNPR 454
           RVHCAEVLGTPIVGDYKYGWQAH+NWKPF  N HKNQGKKQ LPFGLDFESGSIAENNPR
Sbjct: 361 RVHCAEVLGTPIVGDYKYGWQAHRNWKPFPHNSHKNQGKKQSLPFGLDFESGSIAENNPR 420

Query: 455 LHLHCKQLTLPDVSFALHNDSLDHDCSRLECLELDAPLPSYMQKSWDIINS 505
           LHLHCK LTLPDVS ALHNDSLDHDCSRLE LELDAPLPSYMQ+SWDIIN+
Sbjct: 421 LHLHCKHLTLPDVSCALHNDSLDHDCSRLERLELDAPLPSYMQRSWDIINN 471

[Close]
BLAST of Chy1G000990 vs. NCBI nr
Match: KAG7011636.1 (RNA pseudouridine synthase 4, mitochondrial, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 801 bits (2070), Expect = 9.94e-290
Identity = 394/467 (84.37%), Postives = 420/467 (89.94%), Query Frame = 0 

Query: 39  ALFRPSIILRTSPSCTDPTGEILYFSIFHVLKSTLALSNASEPVSQQKSEKGSNRWFTLP 98
           A+ R S+I R+ PS   P GE L+FSIF   KST ALS+A+E VS+QK+EKGSNRWFTLP
Sbjct: 5   AVLRRSVIRRSFPSSAAPGGESLHFSIFCERKSTFALSSATETVSEQKNEKGSNRWFTLP 64

Query: 99  PYTATVNGSIFGNRIMNGGAQAELGASTAAITALKWVIRCCPELPRSLVQKLFRLRKVRR 158
           P+TAT NGS  GNRIMNGGAQAE   ST AITALKWV RCCPELPRSLVQKLFRLRKVRR
Sbjct: 65  PFTATANGSALGNRIMNGGAQAEAETSTTAITALKWVFRCCPELPRSLVQKLFRLRKVRR 124

Query: 159 GSSMIENSEYNLEARDQLKRVAAKDILNVGDQIFLPMSVQVFSTEKTRDYCNKEEETFMH 218
           GSSMIENSE NLEARDQLKRVAA DILN+GD++ LP+SVQVF TE+  D CNKEE+ F+H
Sbjct: 125 GSSMIENSECNLEARDQLKRVAANDILNIGDKLCLPISVQVFPTERAPDCCNKEEKKFIH 184

Query: 219 SLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYGYSEPPRLVHRLDRDSSGI 278
           SLVLYKDPAIL INKPPGLPVQGGIGVKRSLD+LAASCLSY YSEPPRLVHRLDRDSSGI
Sbjct: 185 SLVLYKDPAILAINKPPGLPVQGGIGVKRSLDQLAASCLSYDYSEPPRLVHRLDRDSSGI 244

Query: 279 LIMGRSSTSTSLLHSVFREKTFEASKHANHVDSEKKILLKRYWALVIGSPRRSKGLITAP 338
           LIMGR+STSTS+LHS+FREKT  ASKHANHVDSEKKIL KRYWALVIGSPRR+KGLITAP
Sbjct: 245 LIMGRTSTSTSVLHSIFREKTLGASKHANHVDSEKKILQKRYWALVIGSPRRTKGLITAP 304

Query: 339 LGKIVLDDRRSEQIMVIDNAEPMPSQQAITQYKVIKSHHGYTWLELCPLTGRKHQLRVHC 398
           L K+VLDD RSEQIMVIDNAE MP Q AIT YKVIKSHHGYTWLELCPLTGRKHQLRVHC
Sbjct: 305 LVKMVLDDGRSEQIMVIDNAESMPFQHAITTYKVIKSHHGYTWLELCPLTGRKHQLRVHC 364

Query: 399 AEVLGTPIVGDYKYGWQAHKNWKPFRSNFHKNQGKKQFLPFGLDFESGSIAENNPRLHLH 458
           AEVLGTPIVGDYKYGWQAH+NWKPF  N HKNQGKKQ LPFGLDFESGSIAEN+PRLHLH
Sbjct: 365 AEVLGTPIVGDYKYGWQAHRNWKPFPHNSHKNQGKKQSLPFGLDFESGSIAENSPRLHLH 424

Query: 459 CKQLTLPDVSFALHNDSLDHDCSRLECLELDAPLPSYMQKSWDIINS 505
           CKQLTLPDVS ALHNDSLDHDCSRLE LELDAPLPSYMQ+SWDIIN+
Sbjct: 425 CKQLTLPDVSCALHNDSLDHDCSRLERLELDAPLPSYMQRSWDIINN 471

[Close]
BLAST of Chy1G000990 vs. TAIR 10
Match: AT3G19440.1 (Pseudouridine synthase family protein )

HSP 1 Score: 449.1 bits (1154), Expect = 4.6e-126
Identity = 233/441 (52.83%), Postives = 307/441 (69.61%), Query Frame = 0 

Query: 74  ALSNASEPVSQQKSEKGSNRWFTLPPYTATVNGSIFGNRIMNGGAQAELGASTAAITALK 133
           A   ++   S  +++    +W TLPP++ T++G+  G  +++ G   +     +  TAL+
Sbjct: 35  AAHQSTRSYSTTQTDDSRGKWLTLPPFSPTIDGTAVGKDLLSDGDSVKSSTDNSKTTALR 94

Query: 134 WVIRCCPELPRSLVQKLFRLRKVRRGSSM-IENSEYNLEARDQLKRVAAKDILNVGDQIF 193
           W++RC P+LPR+LVQKLFRLR+VRR  SM ++  E     R QLKRVAAK+ LNVGD+I+
Sbjct: 95  WILRCRPDLPRTLVQKLFRLRQVRREMSMSVDGDELQ---RSQLKRVAAKESLNVGDRIY 154

Query: 194 LPMSV-----QVFSTEKTRDYCNKEEETFMHSLVLYKDPAILVINKPPGLPVQGGIGVKR 253
           LP+SV     Q    +K    C+ EE  F+ SLVLYKDPAI+V+NKP GL VQGG G+K 
Sbjct: 155 LPLSVDNDTPQTPPAKKESFQCSDEERKFVCSLVLYKDPAIIVLNKPHGLAVQGGSGIKT 214

Query: 254 SLDELAASCLSYGYSEPPRLVHRLDRDSSGILIMGRSSTSTSLLHSVFREKTFEASKHAN 313
           S+DELAASCL +  SE PRLVHRLDRD SG+L++ R+ T+ ++LHS+FREKT  AS +  
Sbjct: 215 SIDELAASCLKFDKSESPRLVHRLDRDCSGLLVLARTQTAATVLHSIFREKTTGASAYG- 274

Query: 314 HVDSEKKILLKRYWALVIGSPRRSKGLITAPLGKIVLDDRRSEQIMVIDNAEPMPSQQAI 373
            V    K L ++Y ALVIG P R +G I+APL K+V+DD +SE+I V DN E + +Q AI
Sbjct: 275 -VKKNVKSLKRKYMALVIGCPPRQRGQISAPLRKVVVDDGKSERITVNDNGELVSTQHAI 334

Query: 374 TQYKVIKSH-HGYTWLELCPLTGRKHQLRVHCAEVLGTPIVGDYKYGWQAHKNWKPFRSN 433
           T+Y+VI+S  HGYTWLEL PLTGRKHQLRVHCAEVLGTPIVGDYKYGWQAHK  +PF S+
Sbjct: 335 TEYRVIESSPHGYTWLELRPLTGRKHQLRVHCAEVLGTPIVGDYKYGWQAHKAREPFVSS 394

Query: 434 FHKNQGKKQFLPFGLDFESGSIAENNPRLHLHCKQLTLPDVSFALH--NDSLDHDCSRLE 493
              N  K+   PFGLD + G ++   P LHLH KQ+ LP++S  L     S D D S L+
Sbjct: 395 -ENNPTKQSSSPFGLDLDGGDVSSKQPHLHLHSKQIDLPNISQLLEKMQVSSDSDISDLD 454

Query: 494 CLELDAPLPSYMQKSWDIINS 506
            L+ DAPLPS+MQ S++++ S
Sbjct: 455 SLKFDAPLPSHMQLSFNLLKS 469

[Close]
BLAST of Chy1G000990 vs. TAIR 10
Match: AT1G78910.1 (Pseudouridine synthase family protein )

HSP 1 Score: 295.0 bits (754), Expect = 1.1e-79
Identity = 162/418 (38.76%), Postives = 244/418 (58.37%), Query Frame = 0 

Query: 115 NGGAQAELGASTAAITALKWVIRCCPEL---------PRSLVQKLFRLRKVRRGSSMIEN 174
           N GA  + G S   +TA+ WV     E+          + LVQ  FR R+        + 
Sbjct: 67  NAGADGDDGDSGGHVTAISWVKYYFEEIYDKAIQTHFTKGLVQMEFRGRR--------DA 126

Query: 175 SEYNLEARDQLKRVAAKDILNVGDQIFLPMSVQVFSTEKTRD-------YCNKEEETFMH 234
           S    +    ++++   +++ +GD+I+LP+S+      K  D       Y N +E  ++ 
Sbjct: 127 SREKEDGAIPMRKIKHNEVMQIGDKIWLPVSIAEMRISKRYDTIPSGTLYPNADEIAYLQ 186

Query: 235 SLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYGYSEPPRLVHRLDRDSSGI 294
            LV +KD AI+V+NKPP LPV+G + +  S+D LAA+ LS+G  E PRLVHRLDR++SG+
Sbjct: 187 RLVRFKDSAIIVLNKPPKLPVKGNVPIHNSMDALAAAALSFGNDEGPRLVHRLDRETSGL 246

Query: 295 LIMGRSSTSTSLLHSVFREKTFEASKHANHVDSEKKILLKRYWALVIGSPRRSKGLITAP 354
           L+MGR+  S   LHSVF +     S      +   + + ++YWALVIGSP+  +GLI+AP
Sbjct: 247 LVMGRTKESIDYLHSVFSDYKGRNSS-CKAWNKACEAMYQQYWALVIGSPKEKEGLISAP 306

Query: 355 LGKIVLDDRRSEQIMVIDNAEPMPSQQAITQYKVI-KSHHGYTWLELCPLTGRKHQLRVH 414
           L K++LDD +++++++   +    SQ AIT+YKV+    +G +W+EL P+T RKHQLRVH
Sbjct: 307 LSKVLLDDGKTDRVVLAQGSGFEASQDAITEYKVLGPKINGCSWVELRPITSRKHQLRVH 366

Query: 415 CAEVLGTPIVGDYKYGWQAHKNWKPF-RSNFHKNQGKKQFL--PFGLDFESGSIAENNPR 474
           CAE LGTPIVGDYKYGW  HK WK   + +     GK   L  P GLD + GS+    P 
Sbjct: 367 CAEALGTPIVGDYKYGWFVHKRWKQMPQVDIEPTTGKPYKLRRPEGLDVQKGSVLSKVPL 426

Query: 475 LHLHCKQLTLPDVSFALHNDSLDHD-------CSRLECLELDAPLPSYMQKSWDIINS 506
           LHLHC+++ LP+++  LH  +             + + L   A +PS+M+ SW++++S
Sbjct: 427 LHLHCREMVLPNIAKFLHVMNQQETEPLHTGIIDKPDLLRFVASMPSHMKISWNLMSS 475

[Close]
BLAST of Chy1G000990 vs. TAIR 10
Match: AT1G76050.2 (Pseudouridine synthase family protein )

HSP 1 Score: 73.6 bits (179), Expect = 5.3e-13
Identity = 83/346 (23.99%), Postives = 140/346 (40.46%), Query Frame = 0 

Query: 97  LPPYTATVNGSIFGNRIMNGGAQAE--LGASTAAITALKWVIRCCPELPRSLVQKLFRLR 156
           +P   A     I   R+ N G + E  + A+   I    W+      + R+ VQ   RL 
Sbjct: 48  IPNLVACSPTEIIIPRVNNAGLRIEEIVDAAKGKIRLDSWISSRINGVSRARVQSSIRLG 107

Query: 157 KVRRGSSMIENSEYNLEARDQLKRVAAKDILNVGDQIFLPMSVQVFSTEKTRDYCNKEEE 216
            V     +++   +N+++ D++    ++           P+  +         Y +K   
Sbjct: 108 LVTVNGRVVDKVSHNVKSGDEVNCTISE---------LQPLKAEAEDIPLDIVYEDK--- 167

Query: 217 TFMHSLVLYKDPAILVINKPPGLP---VQGGIGVKRSL---------------------- 276
              H LV+ K PA +V++  PG P   +  GI    SL                      
Sbjct: 168 ---HVLVVNK-PAHMVVHPAPGNPTGTLVNGILHHCSLPCVDYSNSEEDDDSDEETFSDD 227

Query: 277 DELAASCLSYGYSEPPRLVHRLDRDSSGILIMGRSSTSTSLLHSVFREKTFEASKHANHV 336
           +E+  S  SY  S  P +VHRLD+ ++G+L++ +   S + L   F+  T E        
Sbjct: 228 EEMTTSPSSYAASVRPGIVHRLDKGTTGLLVVAKDEHSHAHLAEQFKLHTIE-------- 287

Query: 337 DSEKKILLKRYWALVIGSPRRSKGLITAPLGKIVLDDRRSEQIMVIDNAEPMPSQQAITQ 396
                   + Y +L  G P   +G I  P+G+      R     +        ++ A ++
Sbjct: 288 --------RVYVSLTTGVPSPPQGRIEIPIGRD--SSNRIRMAAIPGGVRGGRARHAASR 347

Query: 397 YKVIKSH--HGYTWLELCPLTGRKHQLRVHCAEVLGTPIVGDYKYG 414
           YKVI++    G   +E    TGR HQ+R H A+ +G P++GD  YG
Sbjct: 348 YKVIETFAGGGSALVEWRLETGRTHQIRAH-AKYMGVPLLGDEVYG 358

[Close]
BLAST of Chy1G000990 vs. TAIR 10
Match: AT3G52260.2 (Pseudouridine synthase family protein )

HSP 1 Score: 64.3 bits (155), Expect = 3.2e-10
Identity = 61/211 (28.91%), Postives = 99/211 (46.92%), Query Frame = 0 

Query: 211 KEEETFMHSLVLYKDPAILVINKPPGLPV-QGGIGVKRSLDELAASCL----SY-GYSEP 270
           KE +T     VLY+D  ++ +NKP GL V  GG+  +R++      C     SY G  E 
Sbjct: 89  KEPDTPYSLEVLYEDDDLIALNKPSGLQVLPGGLFQQRTVLTQLQWCFGKNDSYIGSRES 148

Query: 271 PR--LVHRLDRDSSGILIMGRSSTSTSLLHSVFREKT-FEASKHANHVDSEKKILLKRYW 330
           P    VHRL R +SGIL+  ++  + + L + F E T    S + +      + L K Y 
Sbjct: 149 PHPVPVHRLGRGTSGILLCAKTKLAKTKLAAYFAEGTSLVGSGNLDQECGTGRKLSKIYR 208

Query: 331 ALVIGSPRRSKGLITAPLGKIVLDDRRSEQIMVIDNAEPMPSQQAITQYKVIKSHHGYTW 390
           AL  G     + +I  P+G +          +     +P  S+  + +    K+    + 
Sbjct: 209 ALADGIVEEDEVVIKQPIGVVRYPGVAQGLYVASPEGKPAFSKVFVLERDREKN---CSL 268

Query: 391 LELCPLTGRKHQLRVHCAEVLGTPIVGDYKY 413
           +++   +GR HQ+R+H A  +G P+VGD  Y
Sbjct: 269 VKVEIQSGRPHQIRIHLA-YMGHPLVGDPLY 295

[Close]
BLAST of Chy1G000990 vs. TAIR 10
Match: AT3G52260.3 (Pseudouridine synthase family protein )

HSP 1 Score: 58.9 bits (141), Expect = 1.3e-08
Identity = 58/206 (28.16%), Postives = 96/206 (46.60%), Query Frame = 0 

Query: 211 KEEETFMHSLVLYKDPAILVINKPPGLPV-QGGIGVKRSLDELAASCL----SY-GYSEP 270
           KE +T     VLY+D  ++ +NKP GL V  GG+  +R++      C     SY G  E 
Sbjct: 119 KEPDTPYSLEVLYEDDDLIALNKPSGLQVLPGGLFQQRTVLTQLQWCFGKNDSYIGSRES 178

Query: 271 PR--LVHRLDRDSSGILIMGRSSTSTSLLHSVFREKT-FEASKHANHVDSEKKILLKRYW 330
           P    VHRL R +SGIL+  ++  + + L + F E T    S + +      + L K Y 
Sbjct: 179 PHPVPVHRLGRGTSGILLCAKTKLAKTKLAAYFAEGTSLVGSGNLDQECGTGRKLSKIYR 238

Query: 331 ALVIGSPRRSKGLITAPLGKIVLDDRRSEQIMVIDNAEPMPSQQAITQYKVIKSHHGYTW 390
           AL  G     + +I  P+G +          +     +P  S+  + +    K+    + 
Sbjct: 239 ALADGIVEEDEVVIKQPIGVVRYPGVAQGLYVASPEGKPAFSKVFVLERDREKN---CSL 298

Query: 391 LELCPLTGRKHQLRVHCAEVLGTPIV 408
           +++   +GR HQ+R+H A  +G P+V
Sbjct: 299 VKVEIQSGRPHQIRIHLA-YMGHPLV 320

The following BLAST results are available for this feature:
BLAST of Chy1G000990 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9LT726.5e-12552.83RNA pseudouridine synthase 4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=A... [more]
Q5XET61.6e-7838.76RNA pseudouridine synthase 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=A... [more]
Q0J4D46.0e-7837.85RNA pseudouridine synthase 3, mitochondrial OS=Oryza sativa subsp. japonica OX=3... [more]
Q69K071.1e-7448.72RNA pseudouridine synthase 4, mitochondrial OS=Oryza sativa subsp. japonica OX=3... [more]
Q9ZDR79.6e-2831.91Ribosomal large subunit pseudouridine synthase C OS=Rickettsia prowazekii (strai... [more]
BLAST of Chy1G000990 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A5D3C4F57.2e-26093.84RNA pseudouridine synthase 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A1S3BZE77.2e-26093.84RNA pseudouridine synthase 4, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103495... [more]
A0A6J1GMP92.2e-22984.08RNA pseudouridine synthase 4, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A6J1IBC55.7e-22582.84RNA pseudouridine synthase 4, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A6J1D4321.7e-22481.78RNA pseudouridine synthase 4, mitochondrial OS=Momordica charantia OX=3673 GN=LO... [more]
BLAST of Chy1G000990 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_011658889.10.097.66RNA pseudouridine synthase 4, mitochondrial [Cucumis sativus] >KGN43951.2 hypoth... [more]
XP_008454996.10.093.84PREDICTED: RNA pseudouridine synthase 4, mitochondrial [Cucumis melo] >KAA003133... [more]
XP_038888757.19.71e-30487.45RNA pseudouridine synthase 4, mitochondrial [Benincasa hispida][more]
XP_023553811.18.55e-29184.08RNA pseudouridine synthase 4, mitochondrial [Cucurbita pepo subsp. pepo][more]
KAG7011636.19.94e-29084.37RNA pseudouridine synthase 4, mitochondrial, partial [Cucurbita argyrosperma sub... [more]
BLAST of Chy1G000990 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G19440.14.6e-12652.83Pseudouridine synthase family protein [more]
AT1G78910.11.1e-7938.76Pseudouridine synthase family protein [more]
AT1G76050.25.3e-1323.99Pseudouridine synthase family protein [more]
AT3G52260.23.2e-1028.91Pseudouridine synthase family protein [more]
AT3G52260.31.3e-0828.16Pseudouridine synthase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.30.2350.10Pseudouridine synthasecoord: 209..472
e-value: 4.6E-53
score: 182.0
NoneNo IPR availablePANTHERPTHR21600MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASEcoord: 80..503
NoneNo IPR availablePANTHERPTHR21600:SF53RNA PSEUDOURIDINE SYNTHASE 3, MITOCHONDRIALcoord: 80..503
IPR006145Pseudouridine synthase, RsuA/RluA-likePFAMPF00849PseudoU_synth_2coord: 228..399
e-value: 1.6E-24
score: 86.8
IPR006145Pseudouridine synthase, RsuA/RluA-likeCDDcd02869PseudoU_synth_RluA_likecoord: 228..424
e-value: 2.51875E-51
score: 170.981
IPR020103Pseudouridine synthase, catalytic domain superfamilySUPERFAMILY55120Pseudouridine synthasecoord: 214..501

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy1G000990.1Chy1G000990.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000455 enzyme-directed rRNA pseudouridine synthesis
biological_process GO:0001522 pseudouridine synthesis
biological_process GO:0009451 RNA modification
molecular_function GO:0009982 pseudouridine synthase activity
molecular_function GO:0003723 RNA binding