Chy1G000510 (gene) Cucumber (hystrix) v1

Overview
NameChy1G000510
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionLAG1 longevity assurance homolog 2
LocationchrH01: 372644 .. 376955 (-)
RNA-Seq ExpressionChy1G000510
SyntenyChy1G000510
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACGACTCGATATGGACCCACACTTCCCCTCCCCACCTTTTGCATTTCTTCATCGCCCTCCACTTCTCCTTTGCCTTCTTTGCCGCCAGGTTCTTACTCGACAGATTCATTTTTCGTGTGAGTTTCTTCTTCTTTTTTTTTTTTTCTTTCTTTTATTCATTTTCCTTCCGATTGTTATGTTTCAATTTATCTACTTTAATGTTTCCTGTTCATGCTGATGAATTGTTTTGTGTGATGCTGGAAATGTGAACAATTTAACACCCACATCGAATTACTTATGACATCTCTCTGGTTCTCCTACTTCCTTCTTTCTCTTTCTTTTCTATGCCGTACATGACCCATGTTGTACTCCAATATATATTTTTTTATTATTTTTTTAATATCTAAGAGCTTGGTGATTTTCGAATGCTACTTCTCTACTCAACTTCATTGGTGTTGGTCGTGTGCTGTGTTTATCCTTTGCTTACTTTGGTCCCTTTAGTATATAGAGCATCTACTGTCTTGGTGACTTGGCCTGCCATGGAATATAAACAATATGTATCAGGCTTTCTTGCCTCTGTTATTTTCATTGAAGATGTCTCAGAAGTTGAGTTGGTCATCATGATGAAATTTTAGAATTTTTACCTTCGTCCTGCTAGCTAAATTTCAGACGATATTCAATTCAAACACAAAAATTAATACAATACAAAAAGATTTTTCAGTGGCTTTTGTTTTGGGCATTGGAAGAACTGAAATTTAGACATCCGAAAAGTAACTAAAGGGAGACATTAAAGTGGCTATACTCCAAACCTCCTCCCACCTATGACACTTGTCCTTAAAAGATCATCCACATCCTCCTCAAAAAGTGGAGTTGAATAGGATTTCCTGCCACGCCACATCTCATTAGAACTACAATCTAGCACAAGTGATGGTTGAAAAGGGAAATGCAAGATAAATTATTCAAATCCTTGTTAGCCTCCTTATAATGATTTGCTTGCAACTACGGTTAAAGAAAAATGGTAGAGGTCGAGCTCTGGGTAGTGTCAACAGTGATGATTCTCTCACCTTTTACTGTATTCTCACTGTTCAACTCATGGGAAAAGATTAATGCATTAGATGGAAAACAGATTACATAGGCTGACCAGTAAATTGTTGAAATTTTGATCCCACTCTATTGTTGCCATGTTGATGATTGCGAGGAGCAAGTGGACAACTTCTTGTTCTTGAGTGTCTCTTCTTCTACTGAAAGAATACAAAGCCCACACTTTATTTTCTCCAAATTCATGATTGAGATGACAGTATCTTTAATTCGTAATTGTCTTAATCAGGGTGAAGCTTCATGCTTTGCACCCTGCTGTATGGATGCACTCAAATAATAAAAACACTGGATTATAAATGTAATTAAGCTAAAGAACCATTCTAGTTTTCATTTAGTTGGGGGTGGGGTGGAGTCTAGCTCCATTTCTTGTTCAGCCAAAGGAGATTTTAAACCTAATATGGATCCCATGAGTTATTAATTTCAATTCCACAACTTATTAATTCTTATACTTTCCCCTAACACTCCCTTCGTATTTAAATTTGGAAAAATGTATAACATGTTTGGTTGACTTTTGAAATTCACATTGTTATTCACTTATTCCTATCCCGGAAATTTTTTTTCACATTGTTGTTTACCTAACATTTTTTCAGTTAAAAGTAAATAATGTGACTGGAAAAACTGTTGCATACCATAGAGCTTTTCTCCTGAGATCCTTTGCCAAAGCATTTTGTAACTATAGGTTATCATTTTTTTAGTAATTCTCTGAACATTGTAGGCTACTTCTTTATCAACCTTTTTTTACTTGGTTCTTTTGCATTCTCAACAAGATTTCCCTGTTATTTATTAAGAAATGGAAAACAATGACGTTATTCTGCATTTTTACTTGTTCTGTTTTCAGAGGTTTGCCATTTGGCTGTTGAGCAAAGGATCTGCTCCACTGAAACTTGATGAGGCTACCCAGTCAAAGGTTGTAAAGTGTTCAGAATCAATGTGGAAACTAGCATACTATGGCACCGTTGAAATATGCATTCTAAAAATTGCATACAATGAACCATGGTTTAGAGATTCAAATCAATACTTCAAAGGCTGGCCAAATCAGGAGTTGCAGTGAGTGGTTTACCTGCTTTAATGTTAATTTAATTGATGACCTTCATCTTTTCATCAACTAGCAGATCTAACTTGCTGTATGTATTGATATTGATTGAAATGCTTGAAGTTGATCGGTGTCTTTTTCTATCTACATCACTTTGTCTTTCCTTCCTTCATCAGTGCTATTGGACTCTTTCCTCCATTCTTGTTCTTCCTTGTTATGTAAACTTCAATACTAGGAAGTTGAACTTCTTACTTGTAACTTTAGCATAAATGAATTAAAGATTTTAGTTGTTTATGTACTCATCCATTTCACTTCTTTCTGCAGACTTCCTCTAAAATTGCTCTATATGTGCCAATGTGGGTTCTACTTATATAGCATCGCTGCACTTCTTATATGGGAGACTCGGAGAAAGGATTTTTCTGTCATGATGTCCCATCATGTAATTACTGTCATTTTGATCGGATACTCATATATAACGAGGTATTATGATACTTGTTTTCTTCGGGTTCAAATGCCTAGTAGTGTTTACTAATAATTAGACATATGCTAGTGGCATTTCACAGATTGGGAAAATCGTGCCAGAGGCTGTTTCCCATTTATGCTTTTATGGACCTTGTTATATTGTTGCTAGGCTGCTTTTTCCCCCATTTTCTTGTCTTACGATGTTCTTTATTACTATGGCTATTTTGCTCATCTTAATTAGGCAATGAGTGCATATTCTGTTGGAGTATTGAAATGTGCTTTTTGACAGTTTGCATGATGACACTCTAGCCATGCGTCATTAAATATAAAATTAGAGCATGATATGGGAAGGAGAGATATTCAAGTTTAAATTTCTTATATTCAGTTAAAAAAGTTGTTTGGTTCTGATAAAGTTTTTTTGTTTTTTCTATCAAAACTAGACACAACTAAATGGCTTTCTATATTTCTACTCAAATTTTGATAAGGTATGTTTGTTCCTTATTTTCTAGATTTTGAATGTAGACCTGATGGTAGTTAATATACTAATAACCTATTAACAACGCGCATATTGATATCAACTATGATGGTAGTGTTTAGTGTCATGAATGATATAGAGAGTTGTGCGAATATGAAATTCAGTAAGAATTTTAGAGTTGTTTTTTTTTTTTTAAATTATTGGTTCTTTTATTTACCTGGATTGAAACAAATGCGAGGACTTAGTCATGAGAACAGAAATCTGTAGATGTGTTTTTGGGAGAATGTACACACCTCTTATAGCCTCAATTTGGGGATCCATGAGTTATTTTTCTTTACTTATTTGCATAACTTCATGTTTGTGTAAGCAGCGGTGAAAATTTTGTTTTAAAGCCTCTTCTGAACCTTTTTTTCATTGGCTATTTTTGGGTGTGTTTAGCCAAAAGAATAGCTGGGCTATTAATTGTTTCTGAGCATCAGAAATTTTTTTTTCTCGACTTTGCAGATTTTTTCAGATTGGATCGGTTATTCTTGCACTACATGATGCAAGTGATGTGTTCATGGAAGCTGCCAAGGTTTTTAAATATTCTGAGAAAGAACTTGGAGCAAGTGTTTTCTTTGGATTTTTTGCTATTTCATGGCTTGTATTGCGGCTTATTTTCTTCCCATTCTGGGTTATAAAAGCAACAAGGTTTGTGCTCCTAATGTTTGATTTATCTTTAATGATTAGGCTCAATACTTGCTCTATCTGATTTTGTGATTGATACTTCTCAGCTATGATCTCTGTGCGTACTTGAAGCTTTCAGATGTGAATTCCAGATTAATATACTACGTTTTCAACACGATGTTGTTGATGTTGCTTGTGTTCCACATATACTGGTGGTTACTCATATGCTCTATGATCAGTAGACAGCTGAAAAATAGGGGAAAAGTTGGAGAGGATATAAGATCTGGTACGTAATTTTACAAATTTGCTTCAAAGTTATCATTGATCAAATAAAAAATGGGGAATCCTCCCACAACTCCCGTCTGAGAATCTCTTAGATTAATGTAGGATAATTTCCTACAAGTTATTGGTACCTATTGTTTTATTCGTCTAATTTCGTTTTTCATACATGGAAAGAGTTTCAGCATAACACGATAAATGCATACTTCGGTTTTCTCTTCTTTTTTTTTTTATTAAAATGGGTAATGGCATGGTTTTAACTGATATTGAAATGTTCTCTTGTATTTTCTTTACAGATTCCGAGGATGAGGATTAG

mRNA sequence

ATGGACGACTCGATATGGACCCACACTTCCCCTCCCCACCTTTTGCATTTCTTCATCGCCCTCCACTTCTCCTTTGCCTTCTTTGCCGCCAGGGTGAAGCTTCATGCTTTGCACCCTGCTAGGTTTGCCATTTGGCTGTTGAGCAAAGGATCTGCTCCACTGAAACTTGATGAGGCTACCCAGTCAAAGGTTGTAAAGTGTTCAGAATCAATGTGGAAACTAGCATACTATGGCACCGTTGAAATATGCATTCTAAAAATTGCATACAATGAACCATGGTTTAGAGATTCAAATCAATACTTCAAAGGCTGGCCAAATCAGGAGTTGCAACTTCCTCTAAAATTGCTCTATATGTGCCAATGTGGGTTCTACTTATATAGCATCGCTGCACTTCTTATATGGGAGACTCGGAGAAAGGATTTTTCTGTCATGATGTCCCATCATGTAATTACTGTCATTTTGATCGGATACTCATATATAACGAGATTTTTTCAGATTGGATCGGTTATTCTTGCACTACATGATGCAAGTGATGTGTTCATGGAAGCTGCCAAGGTTTTTAAATATTCTGAGAAAGAACTTGGAGCAAGTGTTTTCTTTGGATTTTTTGCTATTTCATGGCTTGTATTGCGGCTTATTTTCTTCCCATTCTGGGTTATAAAAGCAACAAGCTATGATCTCTGTGCGTACTTGAAGCTTTCAGATGTGAATTCCAGATTAATATACTACGTTTTCAACACGATGTTGTTGATGTTGCTTGTGTTCCACATATACTGGTGGTTACTCATATGCTCTATGATCAGTAGACAGCTGAAAAATAGGGGAAAAGTTGGAGAGGATATAAGATCTGATTCCGAGGATGAGGATTAG

Coding sequence (CDS)

ATGGACGACTCGATATGGACCCACACTTCCCCTCCCCACCTTTTGCATTTCTTCATCGCCCTCCACTTCTCCTTTGCCTTCTTTGCCGCCAGGGTGAAGCTTCATGCTTTGCACCCTGCTAGGTTTGCCATTTGGCTGTTGAGCAAAGGATCTGCTCCACTGAAACTTGATGAGGCTACCCAGTCAAAGGTTGTAAAGTGTTCAGAATCAATGTGGAAACTAGCATACTATGGCACCGTTGAAATATGCATTCTAAAAATTGCATACAATGAACCATGGTTTAGAGATTCAAATCAATACTTCAAAGGCTGGCCAAATCAGGAGTTGCAACTTCCTCTAAAATTGCTCTATATGTGCCAATGTGGGTTCTACTTATATAGCATCGCTGCACTTCTTATATGGGAGACTCGGAGAAAGGATTTTTCTGTCATGATGTCCCATCATGTAATTACTGTCATTTTGATCGGATACTCATATATAACGAGATTTTTTCAGATTGGATCGGTTATTCTTGCACTACATGATGCAAGTGATGTGTTCATGGAAGCTGCCAAGGTTTTTAAATATTCTGAGAAAGAACTTGGAGCAAGTGTTTTCTTTGGATTTTTTGCTATTTCATGGCTTGTATTGCGGCTTATTTTCTTCCCATTCTGGGTTATAAAAGCAACAAGCTATGATCTCTGTGCGTACTTGAAGCTTTCAGATGTGAATTCCAGATTAATATACTACGTTTTCAACACGATGTTGTTGATGTTGCTTGTGTTCCACATATACTGGTGGTTACTCATATGCTCTATGATCAGTAGACAGCTGAAAAATAGGGGAAAAGTTGGAGAGGATATAAGATCTGATTCCGAGGATGAGGATTAG

Protein sequence

MDDSIWTHTSPPHLLHFFIALHFSFAFFAARVKLHALHPARFAIWLLSKGSAPLKLDEATQSKVVKCSESMWKLAYYGTVEICILKIAYNEPWFRDSNQYFKGWPNQELQLPLKLLYMCQCGFYLYSIAALLIWETRRKDFSVMMSHHVITVILIGYSYITRFFQIGSVILALHDASDVFMEAAKVFKYSEKELGASVFFGFFAISWLVLRLIFFPFWVIKATSYDLCAYLKLSDVNSRLIYYVFNTMLLMLLVFHIYWWLLICSMISRQLKNRGKVGEDIRSDSEDED*
Homology
BLAST of Chy1G000510 vs. ExPASy Swiss-Prot
Match: Q9LJK3 (Ceramide synthase LOH2 OS=Arabidopsis thaliana OX=3702 GN=LOH2 PE=1 SV=1)

HSP 1 Score: 381.3 bits (978), Expect = 9.5e-105
Identity = 185/275 (67.27%), Postives = 223/275 (81.09%), Query Frame = 0

Query: 16  HFFIALHFSFAFFAARVKLHALHPARFAIWLLSKGSAPLKL-DEATQSKVVKCSESMWKL 75
           HF IA++F+F FF  R+ L      R A+WLLS GSAP+KL D AT++K+VKC ES+WKL
Sbjct: 21  HFQIAVYFAFGFFFLRLVLDRYVFQRIALWLLSTGSAPIKLNDAATRAKIVKCKESLWKL 80

Query: 76  AYYGTVEICILKIAYNEPWFRDSNQYFKGWPNQELQLPLKLLYMCQCGFYLYSIAALLIW 135
            YY   +  +L++ Y+EPW RD   YF GWPNQEL+L +KL YMCQCGFY+Y +AALL W
Sbjct: 81  LYYAACDFFVLQVIYHEPWARDIKLYFHGWPNQELKLSIKLYYMCQCGFYVYGVAALLAW 140

Query: 136 ETRRKDFSVMMSHHVITVILIGYSYITRFFQIGSVILALHDASDVFMEAAKVFKYSEKEL 195
           ETRRKDF+VMMSHHVIT+IL+ YSY+T FF+IG++ILALHDASDVFME AK+FKYSEKE 
Sbjct: 141 ETRRKDFAVMMSHHVITIILLSYSYLTSFFRIGAIILALHDASDVFMETAKIFKYSEKEF 200

Query: 196 GASVFFGFFAISWLVLRLIFFPFWVIKATSYDLCAYLKLSDVNSRLIYYVFNTMLLMLLV 255
           GASV F  FA+SWL+LRLI+FPFW+I+ATS +L  YL ++     L+YY FNTMLLMLLV
Sbjct: 201 GASVCFALFAVSWLLLRLIYFPFWIIRATSIELLDYLDMTSAEGTLMYYSFNTMLLMLLV 260

Query: 256 FHIYWWLLICSMISRQLKNRGKVGEDIRSDSEDED 290
           FHIYWW LIC+MI R LKNRGKVGEDIRSDSED+D
Sbjct: 261 FHIYWWYLICAMIVRLLKNRGKVGEDIRSDSEDDD 295

BLAST of Chy1G000510 vs. ExPASy Swiss-Prot
Match: Q84QC0 (ASC1-like protein 3 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0264000 PE=2 SV=1)

HSP 1 Score: 331.3 bits (848), Expect = 1.1e-89
Identity = 168/279 (60.22%), Postives = 208/279 (74.55%), Query Frame = 0

Query: 12  PHLLHFF-IALHFSFAFFAARVKLHALHPARFAIWLLSKGSAPLKLDEATQSKVVKCSES 71
           P    FF + L FS  FF AR  L  L     A +  S  ++ L  DE  Q+K+VK SES
Sbjct: 6   PEASSFFPLTLVFSVGFFCARFFLDRLVYKPLAAYCFSSKASKLMNDEVRQAKIVKFSES 65

Query: 72  MWKLAYYGTVEICILKIAYNEPWFRDSNQYFKGWPNQELQLPLKLLYMCQCGFYLYSIAA 131
           +WKL YYG+V+  +L I   EPW  D+ QYF+GWPNQ +   L L YMCQCGFY+YSI A
Sbjct: 66  IWKLTYYGSVQAWVLLIIKQEPWSLDTMQYFEGWPNQYMTSSLMLFYMCQCGFYIYSIFA 125

Query: 132 LLIWETRRKDFSVMMSHHVITVILIGYSYITRFFQIGSVILALHDASDVFMEAAKVFKYS 191
           L+ WETRRKDF+VMMSHHV+T ILIGY+Y+T FF+IG++ILALHDASDVF+E AK+ KY+
Sbjct: 126 LVAWETRRKDFAVMMSHHVVTSILIGYAYLTGFFRIGTIILALHDASDVFLETAKLCKYT 185

Query: 192 EKELGASVFFGFFAISWLVLRLIFFPFWVIKATSYDLCAYLKLSDVNSRLIYYVFNTMLL 251
           EKELGAS+FFG FA+SWL+LRLI+FPFW+IK +SY     L+  +     +YY+FNTMLL
Sbjct: 186 EKELGASLFFGLFALSWLLLRLIYFPFWIIKTSSYQSIISLRKLEKFPTTLYYIFNTMLL 245

Query: 252 MLLVFHIYWWLLICSMISRQLKNRGKVGEDIRSDSEDED 290
            LLVFHIYWW LIC MI +QL N+G+VGED+RSDSEDE+
Sbjct: 246 TLLVFHIYWWKLICLMIMKQLNNKGQVGEDVRSDSEDEE 284

BLAST of Chy1G000510 vs. ExPASy Swiss-Prot
Match: Q6NQI8 (Ceramide synthase 1 LOH3 OS=Arabidopsis thaliana OX=3702 GN=LOH3 PE=1 SV=2)

HSP 1 Score: 240.4 bits (612), Expect = 2.6e-62
Identity = 132/293 (45.05%), Postives = 182/293 (62.12%), Query Frame = 0

Query: 6   WTHTSPPHLLHFFIALHFSFAFFAARVKLHALHPARFAIWLLSKGSAPLKLDEATQ--SK 65
           W H S P    F +   F+  F + R  L      + A +L+         D+ T+   K
Sbjct: 12  WEHESSPVYQDFRVLPLFAVFFPSIRFLLDRFVFEKLAKYLIYGKHRQDMGDDTTERKKK 71

Query: 66  VVKCSESMWKLAYYGTVEICILKIAYNEPWFRDSNQYFKG-----WPNQELQLPLKLLYM 125
           + K  ES WK  YY + EI  L + YNEPWF ++  ++ G     WP+Q+ +L LKLLYM
Sbjct: 72  IRKFKESAWKCVYYLSAEILALSVTYNEPWFMNTKYFWVGPGDQTWPDQQTKLKLKLLYM 131

Query: 126 CQCGFYLYSIAALLIWETRRKDFSVMMSHHVITVILIGYSYITRFFQIGSVILALHDASD 185
              GFY YSI AL+ WETRR DF V M HH+ T+ILI  SY+  F ++GSV+LALHDASD
Sbjct: 132 FVAGFYTYSIFALVFWETRRSDFGVSMGHHIATLILIVLSYVCSFSRVGSVVLALHDASD 191

Query: 186 VFMEAAKVFKYSEKELGASVFFGFFAISWLVLRLIFFPFWVIKATSYDLCAYLKLS--DV 245
           VF+E  K+ KYS  E  AS  F  F +SW++LRLI++PFW++ +TSY++   L      +
Sbjct: 192 VFLEVGKMSKYSGAERIASFSFILFVLSWIILRLIYYPFWILWSTSYEVVLELDKDKHPI 251

Query: 246 NSRLIYYVFNTMLLMLLVFHIYWWLLICSMISRQLKNRGKVGEDIRSDSEDED 290
              + YY+FNT+L  LLV HIYWW+L+  M+ +Q+++RGK+ ED+RSDSE ED
Sbjct: 252 EGPIYYYMFNTLLYCLLVLHIYWWVLMYRMLVKQIQDRGKLSEDVRSDSEGED 304

BLAST of Chy1G000510 vs. ExPASy Swiss-Prot
Match: Q6EUN0 (ASC1-like protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0581300 PE=2 SV=1)

HSP 1 Score: 237.7 bits (605), Expect = 1.7e-61
Identity = 122/239 (51.05%), Postives = 166/239 (69.46%), Query Frame = 0

Query: 58  EATQSKVVKCSESMWKLAYYGTVEICILKIAYNEPWFRDSNQYFKG-----WPNQELQLP 117
           E T+ K+ K  ES WK  Y+ + EI  L + YNEPWF ++  ++ G     WP+Q+++  
Sbjct: 68  EETRKKIRKFKESAWKCVYFLSGEILSLSVTYNEPWFTNTKYFWVGPGDQVWPDQKIKWK 127

Query: 118 LKLLYMCQCGFYLYSIAALLIWETRRKDFSVMMSHHVITVILIGYSYITRFFQIGSVILA 177
           LK +YM   GFY YSI AL+ WETRR DF V MSHHV TV LI  SY+ RF ++GSV+LA
Sbjct: 128 LKAVYMYAAGFYTYSIFALMFWETRRSDFGVSMSHHVATVALIVLSYVFRFARVGSVVLA 187

Query: 178 LHDASDVFMEAAKVFKYSEKELGASVFFGFFAISWLVLRLIFFPFWVIKATSYDLCAYL- 237
           +HDASDVF+E  K+ KYS  +L A+V F  F +SW++LRL +FPFW++++TSY++   L 
Sbjct: 188 IHDASDVFLEVGKMAKYSHCDLLANVAFLLFVVSWVLLRLTYFPFWILRSTSYEVLLTLD 247

Query: 238 -KLSDVNSRLIYYVFNTMLLMLLVFHIYWWLLICSMISRQLKNRGKVGEDIRSDSEDED 290
            K  + +  + YYVFN++L  LLV HIYWW+LI  M+ RQ+K R  VG+D+RSDSE ED
Sbjct: 248 KKKHNFDGPIYYYVFNSLLFSLLVLHIYWWVLIYRMLVRQIKTR-NVGDDVRSDSEGED 305

BLAST of Chy1G000510 vs. ExPASy Swiss-Prot
Match: Q9LDF2 (Ceramide synthase LOH1 OS=Arabidopsis thaliana OX=3702 GN=LOH1 PE=1 SV=1)

HSP 1 Score: 234.6 bits (597), Expect = 1.4e-60
Identity = 119/233 (51.07%), Postives = 161/233 (69.10%), Query Frame = 0

Query: 63  KVVKCSESMWKLAYYGTVEICILKIAYNEPWFRDSNQYFKG-----WPNQELQLPLKLLY 122
           KV K  ES WK  YY + E+  L + YNEPWF ++  ++ G     WP+Q +++ LK LY
Sbjct: 74  KVRKFKESAWKCIYYLSAELLALSVTYNEPWFSNTLYFWIGPGDQIWPDQPMKMKLKFLY 133

Query: 123 MCQCGFYLYSIAALLIWETRRKDFSVMMSHHVITVILIGYSYITRFFQIGSVILALHDAS 182
           M   GFY YSI AL+ WETRR DF V M HH+ T++LI  SYI R  + GSVILALHDAS
Sbjct: 134 MFAAGFYTYSIFALVFWETRRSDFGVSMGHHITTLVLIVLSYICRLTRAGSVILALHDAS 193

Query: 183 DVFMEAAKVFKYSEKELGASVFFGFFAISWLVLRLIFFPFWVIKATSYDLCAYL-KLSDV 242
           DVF+E  K+ KY   E  AS+ F  FA+SW+VLRLI++PFW++ +TSY +   + K    
Sbjct: 194 DVFLEIGKMSKYCGAESLASISFVLFALSWVVLRLIYYPFWILWSTSYQIIMTVDKEKHP 253

Query: 243 NSRLIYYVFNTMLLMLLVFHIYWWLLICSMISRQLKNRGKVGEDIRSDSEDED 290
           N  ++YY+FNT+L  LLV HI+WW+LI  M+ +Q+++RGK+ ED+RSDSE +D
Sbjct: 254 NGPILYYMFNTLLYFLLVLHIFWWVLIYRMLVKQVQDRGKLSEDVRSDSESDD 306

BLAST of Chy1G000510 vs. ExPASy TrEMBL
Match: A0A0A0K373 (TLC domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G073690 PE=4 SV=1)

HSP 1 Score: 557.4 bits (1435), Expect = 3.6e-155
Identity = 280/289 (96.89%), Postives = 281/289 (97.23%), Query Frame = 0

Query: 1   MDDSIWTHTSPPHLLHFFIALHFSFAFFAARVKLHALHPARFAIWLLSKGSAPLKLDEAT 60
           MDDSIWTHTSPPHLLHFFIALHFSFAFFAAR  L      RFAIWLLSKGSAPLKLDEAT
Sbjct: 1   MDDSIWTHTSPPHLLHFFIALHFSFAFFAARFLLDRFIFRRFAIWLLSKGSAPLKLDEAT 60

Query: 61  QSKVVKCSESMWKLAYYGTVEICILKIAYNEPWFRDSNQYFKGWPNQELQLPLKLLYMCQ 120
           QSKVVKCSESMWKLAYYGTVEICILKIAYNEPWFRDSNQYFKGWPNQELQLPLKLLYMCQ
Sbjct: 61  QSKVVKCSESMWKLAYYGTVEICILKIAYNEPWFRDSNQYFKGWPNQELQLPLKLLYMCQ 120

Query: 121 CGFYLYSIAALLIWETRRKDFSVMMSHHVITVILIGYSYITRFFQIGSVILALHDASDVF 180
           CGFYLYSIAALLIWETRRKDFSVMMSHHVITVILIGYSY+TRFFQIGSVILALHDASDVF
Sbjct: 121 CGFYLYSIAALLIWETRRKDFSVMMSHHVITVILIGYSYMTRFFQIGSVILALHDASDVF 180

Query: 181 MEAAKVFKYSEKELGASVFFGFFAISWLVLRLIFFPFWVIKATSYDLCAYLKLSDVNSRL 240
           MEAAKVFKYSEKELGASVFFGFFAISWLVLRLIFFPFWVIKATSYDLCAYLKLSDVNSRL
Sbjct: 181 MEAAKVFKYSEKELGASVFFGFFAISWLVLRLIFFPFWVIKATSYDLCAYLKLSDVNSRL 240

Query: 241 IYYVFNTMLLMLLVFHIYWWLLICSMISRQLKNRGKVGEDIRSDSEDED 290
           IYYVFNTMLLMLLVFHIYWWLLICSMISRQLKNRGKVGEDIRSDSEDED
Sbjct: 241 IYYVFNTMLLMLLVFHIYWWLLICSMISRQLKNRGKVGEDIRSDSEDED 289

BLAST of Chy1G000510 vs. ExPASy TrEMBL
Match: A0A1S3BZU3 (LAG1 longevity assurance homolog 2 OS=Cucumis melo OX=3656 GN=LOC103495235 PE=4 SV=1)

HSP 1 Score: 555.4 bits (1430), Expect = 1.4e-154
Identity = 279/289 (96.54%), Postives = 280/289 (96.89%), Query Frame = 0

Query: 1   MDDSIWTHTSPPHLLHFFIALHFSFAFFAARVKLHALHPARFAIWLLSKGSAPLKLDEAT 60
           MDDSIWTHTSPPHLLHFFIALHFSFAFFAAR  L      RFAIWLLSKG+APLKLDEAT
Sbjct: 1   MDDSIWTHTSPPHLLHFFIALHFSFAFFAARFLLDRFIFRRFAIWLLSKGAAPLKLDEAT 60

Query: 61  QSKVVKCSESMWKLAYYGTVEICILKIAYNEPWFRDSNQYFKGWPNQELQLPLKLLYMCQ 120
           QSKVVKCSESMWKLAYYGTVEICILKIAYNEPWFRDSNQYFKGWPNQELQLPLKLLYMCQ
Sbjct: 61  QSKVVKCSESMWKLAYYGTVEICILKIAYNEPWFRDSNQYFKGWPNQELQLPLKLLYMCQ 120

Query: 121 CGFYLYSIAALLIWETRRKDFSVMMSHHVITVILIGYSYITRFFQIGSVILALHDASDVF 180
           CGFYLYSIAALLIWETRRKDFSVMMSHHVITVILIGYSYITRFFQIGSVILALHDASDVF
Sbjct: 121 CGFYLYSIAALLIWETRRKDFSVMMSHHVITVILIGYSYITRFFQIGSVILALHDASDVF 180

Query: 181 MEAAKVFKYSEKELGASVFFGFFAISWLVLRLIFFPFWVIKATSYDLCAYLKLSDVNSRL 240
           MEAAKVFKYSEKELGASVFFGFFAISWLVLRLIFFPFWVIKATSYDLC YLKLSDVNSRL
Sbjct: 181 MEAAKVFKYSEKELGASVFFGFFAISWLVLRLIFFPFWVIKATSYDLCEYLKLSDVNSRL 240

Query: 241 IYYVFNTMLLMLLVFHIYWWLLICSMISRQLKNRGKVGEDIRSDSEDED 290
           IYYVFNTMLLMLLVFHIYWWLLICSMISRQLKNRGKVGEDIRSDSEDED
Sbjct: 241 IYYVFNTMLLMLLVFHIYWWLLICSMISRQLKNRGKVGEDIRSDSEDED 289

BLAST of Chy1G000510 vs. ExPASy TrEMBL
Match: A0A6J1EMG2 (LAG1 longevity assurance homolog 2-like OS=Cucurbita moschata OX=3662 GN=LOC111434695 PE=4 SV=1)

HSP 1 Score: 518.5 bits (1334), Expect = 1.8e-143
Identity = 258/287 (89.90%), Postives = 268/287 (93.38%), Query Frame = 0

Query: 3   DSIWTHTSPPHLLHFFIALHFSFAFFAARVKLHALHPARFAIWLLSKGSAPLKLDEATQS 62
           DSIW+HT  P  LHF IA+ F+F F+AAR  L      RFAIWLLSKG+APLKLDEATQS
Sbjct: 2   DSIWSHTGAPDALHFVIAVQFAFGFYAARFLLDRFIFRRFAIWLLSKGAAPLKLDEATQS 61

Query: 63  KVVKCSESMWKLAYYGTVEICILKIAYNEPWFRDSNQYFKGWPNQELQLPLKLLYMCQCG 122
           KVVKCSESMWKLAYYGTVEICILKIAYNEPWFRDSNQYFKGWPNQELQLPLKL YMCQCG
Sbjct: 62  KVVKCSESMWKLAYYGTVEICILKIAYNEPWFRDSNQYFKGWPNQELQLPLKLFYMCQCG 121

Query: 123 FYLYSIAALLIWETRRKDFSVMMSHHVITVILIGYSYITRFFQIGSVILALHDASDVFME 182
           FYLYSIAALLIWETRRKDFSVMMSHHVITVILIGYSY+TRFFQIGSVILALHDASDVFME
Sbjct: 122 FYLYSIAALLIWETRRKDFSVMMSHHVITVILIGYSYMTRFFQIGSVILALHDASDVFME 181

Query: 183 AAKVFKYSEKELGASVFFGFFAISWLVLRLIFFPFWVIKATSYDLCAYLKLSDVNSRLIY 242
           AAKVFKYSEKELGASVFFGFFA++WLVLRLIFFPFWVIK+TSYDLC YLKLSD NSRLIY
Sbjct: 182 AAKVFKYSEKELGASVFFGFFAVTWLVLRLIFFPFWVIKSTSYDLCEYLKLSDANSRLIY 241

Query: 243 YVFNTMLLMLLVFHIYWWLLICSMISRQLKNRGKVGEDIRSDSEDED 290
           YVFNTMLLMLLVFHIYWWLLICSMI RQLKNRGKVGEDIRSDSED+D
Sbjct: 242 YVFNTMLLMLLVFHIYWWLLICSMIHRQLKNRGKVGEDIRSDSEDDD 288

BLAST of Chy1G000510 vs. ExPASy TrEMBL
Match: A0A6J1GMN8 (LAG1 longevity assurance homolog 2-like OS=Cucurbita moschata OX=3662 GN=LOC111455365 PE=4 SV=1)

HSP 1 Score: 518.1 bits (1333), Expect = 2.4e-143
Identity = 259/287 (90.24%), Postives = 268/287 (93.38%), Query Frame = 0

Query: 3   DSIWTHTSPPHLLHFFIALHFSFAFFAARVKLHALHPARFAIWLLSKGSAPLKLDEATQS 62
           DSIW+H S P  LHF IA+ F+F+FFAAR  L      RFAIWLLSKG+APLKLDEATQS
Sbjct: 2   DSIWSHRSAPEALHFVIAIQFAFSFFAARFLLDRFIFRRFAIWLLSKGAAPLKLDEATQS 61

Query: 63  KVVKCSESMWKLAYYGTVEICILKIAYNEPWFRDSNQYFKGWPNQELQLPLKLLYMCQCG 122
           K+VKCSESMWKLAYYGTVEIC+LKIAYN+PWFRDSNQYFKGWPN ELQLPLKLLYMCQCG
Sbjct: 62  KIVKCSESMWKLAYYGTVEICVLKIAYNQPWFRDSNQYFKGWPNAELQLPLKLLYMCQCG 121

Query: 123 FYLYSIAALLIWETRRKDFSVMMSHHVITVILIGYSYITRFFQIGSVILALHDASDVFME 182
           FYLYSIAALLIWETRRKDFSVMMSHHVITVILIGYSYITRFFQIGSVILALHDASDVFME
Sbjct: 122 FYLYSIAALLIWETRRKDFSVMMSHHVITVILIGYSYITRFFQIGSVILALHDASDVFME 181

Query: 183 AAKVFKYSEKELGASVFFGFFAISWLVLRLIFFPFWVIKATSYDLCAYLKLSDVNSRLIY 242
           AAKVFKYSE ELGASVFFG FAISWL+LRLIFFPFWVIKATSYDLC YLKLSDVNSR IY
Sbjct: 182 AAKVFKYSENELGASVFFGCFAISWLILRLIFFPFWVIKATSYDLCEYLKLSDVNSRSIY 241

Query: 243 YVFNTMLLMLLVFHIYWWLLICSMISRQLKNRGKVGEDIRSDSEDED 290
           YVFNTMLLMLLVFHIYWWLLICSMISRQLKNRGKVGEDIRSDSEDED
Sbjct: 242 YVFNTMLLMLLVFHIYWWLLICSMISRQLKNRGKVGEDIRSDSEDED 288

BLAST of Chy1G000510 vs. ExPASy TrEMBL
Match: A0A6J1I601 (LAG1 longevity assurance homolog 2-like OS=Cucurbita maxima OX=3661 GN=LOC111471005 PE=4 SV=1)

HSP 1 Score: 516.9 bits (1330), Expect = 5.4e-143
Identity = 258/287 (89.90%), Postives = 268/287 (93.38%), Query Frame = 0

Query: 3   DSIWTHTSPPHLLHFFIALHFSFAFFAARVKLHALHPARFAIWLLSKGSAPLKLDEATQS 62
           DSIW+H S P  LHF IA+ F+F+FFAAR  L      RFAIWLL+KG+APLKLDEATQS
Sbjct: 2   DSIWSHRSAPEALHFVIAIQFAFSFFAARFLLDRFIFRRFAIWLLNKGAAPLKLDEATQS 61

Query: 63  KVVKCSESMWKLAYYGTVEICILKIAYNEPWFRDSNQYFKGWPNQELQLPLKLLYMCQCG 122
           K+VKCSESMWKLAYYGTVEIC+LKIAYN+PWFRDSNQYFKGWPN ELQLPLKLLYMCQCG
Sbjct: 62  KIVKCSESMWKLAYYGTVEICVLKIAYNQPWFRDSNQYFKGWPNAELQLPLKLLYMCQCG 121

Query: 123 FYLYSIAALLIWETRRKDFSVMMSHHVITVILIGYSYITRFFQIGSVILALHDASDVFME 182
           FYLYSIAALLIWETRRKDFSVMMSHHVITVILIGYSYITRFFQIGSVILALHDASDVFME
Sbjct: 122 FYLYSIAALLIWETRRKDFSVMMSHHVITVILIGYSYITRFFQIGSVILALHDASDVFME 181

Query: 183 AAKVFKYSEKELGASVFFGFFAISWLVLRLIFFPFWVIKATSYDLCAYLKLSDVNSRLIY 242
           AAKVFKYSE ELGASVFFG FAISWL+LRLIFFPFWVIKATSYDLC YLKLSDVNSR IY
Sbjct: 182 AAKVFKYSENELGASVFFGCFAISWLILRLIFFPFWVIKATSYDLCEYLKLSDVNSRSIY 241

Query: 243 YVFNTMLLMLLVFHIYWWLLICSMISRQLKNRGKVGEDIRSDSEDED 290
           YVFNTMLLMLLVFHIYWWLLICSMISRQLKNRGKVGEDIRSDSEDED
Sbjct: 242 YVFNTMLLMLLVFHIYWWLLICSMISRQLKNRGKVGEDIRSDSEDED 288

BLAST of Chy1G000510 vs. NCBI nr
Match: XP_004136982.1 (LAG1 longevity assurance homolog 2 [Cucumis sativus] >KGN43933.1 hypothetical protein Csa_017152 [Cucumis sativus])

HSP 1 Score: 554 bits (1427), Expect = 1.89e-198
Identity = 280/289 (96.89%), Postives = 281/289 (97.23%), Query Frame = 0

Query: 1   MDDSIWTHTSPPHLLHFFIALHFSFAFFAARVKLHALHPARFAIWLLSKGSAPLKLDEAT 60
           MDDSIWTHTSPPHLLHFFIALHFSFAFFAAR  L      RFAIWLLSKGSAPLKLDEAT
Sbjct: 1   MDDSIWTHTSPPHLLHFFIALHFSFAFFAARFLLDRFIFRRFAIWLLSKGSAPLKLDEAT 60

Query: 61  QSKVVKCSESMWKLAYYGTVEICILKIAYNEPWFRDSNQYFKGWPNQELQLPLKLLYMCQ 120
           QSKVVKCSESMWKLAYYGTVEICILKIAYNEPWFRDSNQYFKGWPNQELQLPLKLLYMCQ
Sbjct: 61  QSKVVKCSESMWKLAYYGTVEICILKIAYNEPWFRDSNQYFKGWPNQELQLPLKLLYMCQ 120

Query: 121 CGFYLYSIAALLIWETRRKDFSVMMSHHVITVILIGYSYITRFFQIGSVILALHDASDVF 180
           CGFYLYSIAALLIWETRRKDFSVMMSHHVITVILIGYSY+TRFFQIGSVILALHDASDVF
Sbjct: 121 CGFYLYSIAALLIWETRRKDFSVMMSHHVITVILIGYSYMTRFFQIGSVILALHDASDVF 180

Query: 181 MEAAKVFKYSEKELGASVFFGFFAISWLVLRLIFFPFWVIKATSYDLCAYLKLSDVNSRL 240
           MEAAKVFKYSEKELGASVFFGFFAISWLVLRLIFFPFWVIKATSYDLCAYLKLSDVNSRL
Sbjct: 181 MEAAKVFKYSEKELGASVFFGFFAISWLVLRLIFFPFWVIKATSYDLCAYLKLSDVNSRL 240

Query: 241 IYYVFNTMLLMLLVFHIYWWLLICSMISRQLKNRGKVGEDIRSDSEDED 289
           IYYVFNTMLLMLLVFHIYWWLLICSMISRQLKNRGKVGEDIRSDSEDED
Sbjct: 241 IYYVFNTMLLMLLVFHIYWWLLICSMISRQLKNRGKVGEDIRSDSEDED 289

BLAST of Chy1G000510 vs. NCBI nr
Match: XP_008454946.1 (PREDICTED: LAG1 longevity assurance homolog 2 [Cucumis melo])

HSP 1 Score: 552 bits (1422), Expect = 1.09e-197
Identity = 279/289 (96.54%), Postives = 280/289 (96.89%), Query Frame = 0

Query: 1   MDDSIWTHTSPPHLLHFFIALHFSFAFFAARVKLHALHPARFAIWLLSKGSAPLKLDEAT 60
           MDDSIWTHTSPPHLLHFFIALHFSFAFFAAR  L      RFAIWLLSKG+APLKLDEAT
Sbjct: 1   MDDSIWTHTSPPHLLHFFIALHFSFAFFAARFLLDRFIFRRFAIWLLSKGAAPLKLDEAT 60

Query: 61  QSKVVKCSESMWKLAYYGTVEICILKIAYNEPWFRDSNQYFKGWPNQELQLPLKLLYMCQ 120
           QSKVVKCSESMWKLAYYGTVEICILKIAYNEPWFRDSNQYFKGWPNQELQLPLKLLYMCQ
Sbjct: 61  QSKVVKCSESMWKLAYYGTVEICILKIAYNEPWFRDSNQYFKGWPNQELQLPLKLLYMCQ 120

Query: 121 CGFYLYSIAALLIWETRRKDFSVMMSHHVITVILIGYSYITRFFQIGSVILALHDASDVF 180
           CGFYLYSIAALLIWETRRKDFSVMMSHHVITVILIGYSYITRFFQIGSVILALHDASDVF
Sbjct: 121 CGFYLYSIAALLIWETRRKDFSVMMSHHVITVILIGYSYITRFFQIGSVILALHDASDVF 180

Query: 181 MEAAKVFKYSEKELGASVFFGFFAISWLVLRLIFFPFWVIKATSYDLCAYLKLSDVNSRL 240
           MEAAKVFKYSEKELGASVFFGFFAISWLVLRLIFFPFWVIKATSYDLC YLKLSDVNSRL
Sbjct: 181 MEAAKVFKYSEKELGASVFFGFFAISWLVLRLIFFPFWVIKATSYDLCEYLKLSDVNSRL 240

Query: 241 IYYVFNTMLLMLLVFHIYWWLLICSMISRQLKNRGKVGEDIRSDSEDED 289
           IYYVFNTMLLMLLVFHIYWWLLICSMISRQLKNRGKVGEDIRSDSEDED
Sbjct: 241 IYYVFNTMLLMLLVFHIYWWLLICSMISRQLKNRGKVGEDIRSDSEDED 289

BLAST of Chy1G000510 vs. NCBI nr
Match: XP_038888555.1 (LAG1 longevity assurance homolog 2 [Benincasa hispida])

HSP 1 Score: 530 bits (1364), Expect = 7.28e-189
Identity = 266/287 (92.68%), Postives = 273/287 (95.12%), Query Frame = 0

Query: 3   DSIWTHTSPPHLLHFFIALHFSFAFFAARVKLHALHPARFAIWLLSKGSAPLKLDEATQS 62
           DSIW+HT+PP   HF IA+HF+FAFFAAR  L      R AIWLLSKG+APLKLDEATQS
Sbjct: 2   DSIWSHTTPPDSFHFLIAVHFAFAFFAARFLLDRFIFRRLAIWLLSKGAAPLKLDEATQS 61

Query: 63  KVVKCSESMWKLAYYGTVEICILKIAYNEPWFRDSNQYFKGWPNQELQLPLKLLYMCQCG 122
           KVVKCSESMWKLAYYGTVEICILKIAYNEPWFRDSNQYFKGWPNQELQLPLKLLYMCQCG
Sbjct: 62  KVVKCSESMWKLAYYGTVEICILKIAYNEPWFRDSNQYFKGWPNQELQLPLKLLYMCQCG 121

Query: 123 FYLYSIAALLIWETRRKDFSVMMSHHVITVILIGYSYITRFFQIGSVILALHDASDVFME 182
           FYLYSIAALLIWETRRKDFSVMMSHHVITV+LIGYSYITRFFQIGSVILALHDASDVFME
Sbjct: 122 FYLYSIAALLIWETRRKDFSVMMSHHVITVVLIGYSYITRFFQIGSVILALHDASDVFME 181

Query: 183 AAKVFKYSEKELGASVFFGFFAISWLVLRLIFFPFWVIKATSYDLCAYLKLSDVNSRLIY 242
           AAKVFKYSEKELGASVFFGFFAISWL+LRLIFFPFWVIKATSYDLC YLKLSDVNSRLIY
Sbjct: 182 AAKVFKYSEKELGASVFFGFFAISWLILRLIFFPFWVIKATSYDLCEYLKLSDVNSRLIY 241

Query: 243 YVFNTMLLMLLVFHIYWWLLICSMISRQLKNRGKVGEDIRSDSEDED 289
           YVFNTMLLMLLVFHIYWWLLICSMISRQLKNRGKVGEDIRSDSEDED
Sbjct: 242 YVFNTMLLMLLVFHIYWWLLICSMISRQLKNRGKVGEDIRSDSEDED 288

BLAST of Chy1G000510 vs. NCBI nr
Match: KAG6588659.1 (LAG1 longevity assurance-like 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 516 bits (1329), Expect = 1.57e-183
Identity = 259/287 (90.24%), Postives = 268/287 (93.38%), Query Frame = 0

Query: 3   DSIWTHTSPPHLLHFFIALHFSFAFFAARVKLHALHPARFAIWLLSKGSAPLKLDEATQS 62
           DSIW+HT  P  LHF IA+ F+F F+AAR  L      RFAIWLLSKG+APLKLDEATQS
Sbjct: 2   DSIWSHTGAPDALHFVIAVQFAFGFYAARFLLDRFIFRRFAIWLLSKGAAPLKLDEATQS 61

Query: 63  KVVKCSESMWKLAYYGTVEICILKIAYNEPWFRDSNQYFKGWPNQELQLPLKLLYMCQCG 122
           KVVKCSESMWKLAYYGTVEICILKIAYNEPWFRDSNQYFKGWPNQELQLPLKL YMCQCG
Sbjct: 62  KVVKCSESMWKLAYYGTVEICILKIAYNEPWFRDSNQYFKGWPNQELQLPLKLFYMCQCG 121

Query: 123 FYLYSIAALLIWETRRKDFSVMMSHHVITVILIGYSYITRFFQIGSVILALHDASDVFME 182
           FYLYSIAALLIWETRRKDFSVMMSHHVITVILIGYSY+TRFFQIGSVILALHDASDVFME
Sbjct: 122 FYLYSIAALLIWETRRKDFSVMMSHHVITVILIGYSYMTRFFQIGSVILALHDASDVFME 181

Query: 183 AAKVFKYSEKELGASVFFGFFAISWLVLRLIFFPFWVIKATSYDLCAYLKLSDVNSRLIY 242
           AAKVFKYSEKELGASVFFGFFA++WLVLRLIFFPFWVIKATSYDLC YLKLSD NSRLIY
Sbjct: 182 AAKVFKYSEKELGASVFFGFFAVTWLVLRLIFFPFWVIKATSYDLCEYLKLSDANSRLIY 241

Query: 243 YVFNTMLLMLLVFHIYWWLLICSMISRQLKNRGKVGEDIRSDSEDED 289
           YVFNTMLLMLLVFHIYWWLLICSMI RQLKNRGKVGEDIRSDSED+D
Sbjct: 242 YVFNTMLLMLLVFHIYWWLLICSMIHRQLKNRGKVGEDIRSDSEDDD 288

BLAST of Chy1G000510 vs. NCBI nr
Match: XP_022927943.1 (LAG1 longevity assurance homolog 2-like [Cucurbita moschata] >XP_023530121.1 LAG1 longevity assurance homolog 2-like [Cucurbita pepo subsp. pepo] >KAG7022446.1 LAG1 longevity assurance-like 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 515 bits (1326), Expect = 4.50e-183
Identity = 258/287 (89.90%), Postives = 268/287 (93.38%), Query Frame = 0

Query: 3   DSIWTHTSPPHLLHFFIALHFSFAFFAARVKLHALHPARFAIWLLSKGSAPLKLDEATQS 62
           DSIW+HT  P  LHF IA+ F+F F+AAR  L      RFAIWLLSKG+APLKLDEATQS
Sbjct: 2   DSIWSHTGAPDALHFVIAVQFAFGFYAARFLLDRFIFRRFAIWLLSKGAAPLKLDEATQS 61

Query: 63  KVVKCSESMWKLAYYGTVEICILKIAYNEPWFRDSNQYFKGWPNQELQLPLKLLYMCQCG 122
           KVVKCSESMWKLAYYGTVEICILKIAYNEPWFRDSNQYFKGWPNQELQLPLKL YMCQCG
Sbjct: 62  KVVKCSESMWKLAYYGTVEICILKIAYNEPWFRDSNQYFKGWPNQELQLPLKLFYMCQCG 121

Query: 123 FYLYSIAALLIWETRRKDFSVMMSHHVITVILIGYSYITRFFQIGSVILALHDASDVFME 182
           FYLYSIAALLIWETRRKDFSVMMSHHVITVILIGYSY+TRFFQIGSVILALHDASDVFME
Sbjct: 122 FYLYSIAALLIWETRRKDFSVMMSHHVITVILIGYSYMTRFFQIGSVILALHDASDVFME 181

Query: 183 AAKVFKYSEKELGASVFFGFFAISWLVLRLIFFPFWVIKATSYDLCAYLKLSDVNSRLIY 242
           AAKVFKYSEKELGASVFFGFFA++WLVLRLIFFPFWVIK+TSYDLC YLKLSD NSRLIY
Sbjct: 182 AAKVFKYSEKELGASVFFGFFAVTWLVLRLIFFPFWVIKSTSYDLCEYLKLSDANSRLIY 241

Query: 243 YVFNTMLLMLLVFHIYWWLLICSMISRQLKNRGKVGEDIRSDSEDED 289
           YVFNTMLLMLLVFHIYWWLLICSMI RQLKNRGKVGEDIRSDSED+D
Sbjct: 242 YVFNTMLLMLLVFHIYWWLLICSMIHRQLKNRGKVGEDIRSDSEDDD 288

BLAST of Chy1G000510 vs. TAIR 10
Match: AT3G19260.1 (LAG1 homologue 2 )

HSP 1 Score: 381.3 bits (978), Expect = 6.8e-106
Identity = 185/275 (67.27%), Postives = 223/275 (81.09%), Query Frame = 0

Query: 16  HFFIALHFSFAFFAARVKLHALHPARFAIWLLSKGSAPLKL-DEATQSKVVKCSESMWKL 75
           HF IA++F+F FF  R+ L      R A+WLLS GSAP+KL D AT++K+VKC ES+WKL
Sbjct: 21  HFQIAVYFAFGFFFLRLVLDRYVFQRIALWLLSTGSAPIKLNDAATRAKIVKCKESLWKL 80

Query: 76  AYYGTVEICILKIAYNEPWFRDSNQYFKGWPNQELQLPLKLLYMCQCGFYLYSIAALLIW 135
            YY   +  +L++ Y+EPW RD   YF GWPNQEL+L +KL YMCQCGFY+Y +AALL W
Sbjct: 81  LYYAACDFFVLQVIYHEPWARDIKLYFHGWPNQELKLSIKLYYMCQCGFYVYGVAALLAW 140

Query: 136 ETRRKDFSVMMSHHVITVILIGYSYITRFFQIGSVILALHDASDVFMEAAKVFKYSEKEL 195
           ETRRKDF+VMMSHHVIT+IL+ YSY+T FF+IG++ILALHDASDVFME AK+FKYSEKE 
Sbjct: 141 ETRRKDFAVMMSHHVITIILLSYSYLTSFFRIGAIILALHDASDVFMETAKIFKYSEKEF 200

Query: 196 GASVFFGFFAISWLVLRLIFFPFWVIKATSYDLCAYLKLSDVNSRLIYYVFNTMLLMLLV 255
           GASV F  FA+SWL+LRLI+FPFW+I+ATS +L  YL ++     L+YY FNTMLLMLLV
Sbjct: 201 GASVCFALFAVSWLLLRLIYFPFWIIRATSIELLDYLDMTSAEGTLMYYSFNTMLLMLLV 260

Query: 256 FHIYWWLLICSMISRQLKNRGKVGEDIRSDSEDED 290
           FHIYWW LIC+MI R LKNRGKVGEDIRSDSED+D
Sbjct: 261 FHIYWWYLICAMIVRLLKNRGKVGEDIRSDSEDDD 295

BLAST of Chy1G000510 vs. TAIR 10
Match: AT1G13580.2 (LAG1 longevity assurance homolog 3 )

HSP 1 Score: 240.4 bits (612), Expect = 1.9e-63
Identity = 132/293 (45.05%), Postives = 182/293 (62.12%), Query Frame = 0

Query: 6   WTHTSPPHLLHFFIALHFSFAFFAARVKLHALHPARFAIWLLSKGSAPLKLDEATQ--SK 65
           W H S P    F +   F+  F + R  L      + A +L+         D+ T+   K
Sbjct: 12  WEHESSPVYQDFRVLPLFAVFFPSIRFLLDRFVFEKLAKYLIYGKHRQDMGDDTTERKKK 71

Query: 66  VVKCSESMWKLAYYGTVEICILKIAYNEPWFRDSNQYFKG-----WPNQELQLPLKLLYM 125
           + K  ES WK  YY + EI  L + YNEPWF ++  ++ G     WP+Q+ +L LKLLYM
Sbjct: 72  IRKFKESAWKCVYYLSAEILALSVTYNEPWFMNTKYFWVGPGDQTWPDQQTKLKLKLLYM 131

Query: 126 CQCGFYLYSIAALLIWETRRKDFSVMMSHHVITVILIGYSYITRFFQIGSVILALHDASD 185
              GFY YSI AL+ WETRR DF V M HH+ T+ILI  SY+  F ++GSV+LALHDASD
Sbjct: 132 FVAGFYTYSIFALVFWETRRSDFGVSMGHHIATLILIVLSYVCSFSRVGSVVLALHDASD 191

Query: 186 VFMEAAKVFKYSEKELGASVFFGFFAISWLVLRLIFFPFWVIKATSYDLCAYLKLS--DV 245
           VF+E  K+ KYS  E  AS  F  F +SW++LRLI++PFW++ +TSY++   L      +
Sbjct: 192 VFLEVGKMSKYSGAERIASFSFILFVLSWIILRLIYYPFWILWSTSYEVVLELDKDKHPI 251

Query: 246 NSRLIYYVFNTMLLMLLVFHIYWWLLICSMISRQLKNRGKVGEDIRSDSEDED 290
              + YY+FNT+L  LLV HIYWW+L+  M+ +Q+++RGK+ ED+RSDSE ED
Sbjct: 252 EGPIYYYMFNTLLYCLLVLHIYWWVLMYRMLVKQIQDRGKLSEDVRSDSEGED 304

BLAST of Chy1G000510 vs. TAIR 10
Match: AT1G13580.3 (LAG1 longevity assurance homolog 3 )

HSP 1 Score: 240.4 bits (612), Expect = 1.9e-63
Identity = 132/293 (45.05%), Postives = 182/293 (62.12%), Query Frame = 0

Query: 6   WTHTSPPHLLHFFIALHFSFAFFAARVKLHALHPARFAIWLLSKGSAPLKLDEATQ--SK 65
           W H S P    F +   F+  F + R  L      + A +L+         D+ T+   K
Sbjct: 12  WEHESSPVYQDFRVLPLFAVFFPSIRFLLDRFVFEKLAKYLIYGKHRQDMGDDTTERKKK 71

Query: 66  VVKCSESMWKLAYYGTVEICILKIAYNEPWFRDSNQYFKG-----WPNQELQLPLKLLYM 125
           + K  ES WK  YY + EI  L + YNEPWF ++  ++ G     WP+Q+ +L LKLLYM
Sbjct: 72  IRKFKESAWKCVYYLSAEILALSVTYNEPWFMNTKYFWVGPGDQTWPDQQTKLKLKLLYM 131

Query: 126 CQCGFYLYSIAALLIWETRRKDFSVMMSHHVITVILIGYSYITRFFQIGSVILALHDASD 185
              GFY YSI AL+ WETRR DF V M HH+ T+ILI  SY+  F ++GSV+LALHDASD
Sbjct: 132 FVAGFYTYSIFALVFWETRRSDFGVSMGHHIATLILIVLSYVCSFSRVGSVVLALHDASD 191

Query: 186 VFMEAAKVFKYSEKELGASVFFGFFAISWLVLRLIFFPFWVIKATSYDLCAYLKLS--DV 245
           VF+E  K+ KYS  E  AS  F  F +SW++LRLI++PFW++ +TSY++   L      +
Sbjct: 192 VFLEVGKMSKYSGAERIASFSFILFVLSWIILRLIYYPFWILWSTSYEVVLELDKDKHPI 251

Query: 246 NSRLIYYVFNTMLLMLLVFHIYWWLLICSMISRQLKNRGKVGEDIRSDSEDED 290
              + YY+FNT+L  LLV HIYWW+L+  M+ +Q+++RGK+ ED+RSDSE ED
Sbjct: 252 EGPIYYYMFNTLLYCLLVLHIYWWVLMYRMLVKQIQDRGKLSEDVRSDSEGED 304

BLAST of Chy1G000510 vs. TAIR 10
Match: AT3G25540.1 (TRAM, LAG1 and CLN8 (TLC) lipid-sensing domain containing protein )

HSP 1 Score: 234.6 bits (597), Expect = 1.0e-61
Identity = 119/233 (51.07%), Postives = 161/233 (69.10%), Query Frame = 0

Query: 63  KVVKCSESMWKLAYYGTVEICILKIAYNEPWFRDSNQYFKG-----WPNQELQLPLKLLY 122
           KV K  ES WK  YY + E+  L + YNEPWF ++  ++ G     WP+Q +++ LK LY
Sbjct: 74  KVRKFKESAWKCIYYLSAELLALSVTYNEPWFSNTLYFWIGPGDQIWPDQPMKMKLKFLY 133

Query: 123 MCQCGFYLYSIAALLIWETRRKDFSVMMSHHVITVILIGYSYITRFFQIGSVILALHDAS 182
           M   GFY YSI AL+ WETRR DF V M HH+ T++LI  SYI R  + GSVILALHDAS
Sbjct: 134 MFAAGFYTYSIFALVFWETRRSDFGVSMGHHITTLVLIVLSYICRLTRAGSVILALHDAS 193

Query: 183 DVFMEAAKVFKYSEKELGASVFFGFFAISWLVLRLIFFPFWVIKATSYDLCAYL-KLSDV 242
           DVF+E  K+ KY   E  AS+ F  FA+SW+VLRLI++PFW++ +TSY +   + K    
Sbjct: 194 DVFLEIGKMSKYCGAESLASISFVLFALSWVVLRLIYYPFWILWSTSYQIIMTVDKEKHP 253

Query: 243 NSRLIYYVFNTMLLMLLVFHIYWWLLICSMISRQLKNRGKVGEDIRSDSEDED 290
           N  ++YY+FNT+L  LLV HI+WW+LI  M+ +Q+++RGK+ ED+RSDSE +D
Sbjct: 254 NGPILYYMFNTLLYFLLVLHIFWWVLIYRMLVKQVQDRGKLSEDVRSDSESDD 306

BLAST of Chy1G000510 vs. TAIR 10
Match: AT1G26200.1 (TRAM, LAG1 and CLN8 (TLC) lipid-sensing domain containing protein )

HSP 1 Score: 206.5 bits (524), Expect = 3.0e-53
Identity = 124/298 (41.61%), Postives = 171/298 (57.38%), Query Frame = 0

Query: 6   WTHTSPPHLLHFFIALHFSFAFFAARVKLHALHPARFAIWLLSKGSAPLKLDEATQSKVV 65
           W   S P    F + + F+  F   R+ L      R A  L+         +E  + KVV
Sbjct: 11  WDQESYPDSSDFLVLIFFAPFFLFLRLILDRCIFERVARRLVVPKGLCADSNE-RRKKVV 70

Query: 66  KCSESMWKLAYYGTVEICILKIAYNEPWFRDSNQYFKG-----WPNQELQLPLKLLYMCQ 125
           K  ES WK     +VE   L + Y EPWF+D+  ++ G     WP+Q+++L +K +YM  
Sbjct: 71  KFKESAWKCLCSFSVEAFALYVTYKEPWFKDTRSFWLGPGDQVWPDQKIKLKMKGMYMFV 130

Query: 126 CGFYLYSIAALLIWETRRKDFSVMMSHHVITVILIGYSYITRFFQIGSVILALHDASDVF 185
            G  +Y+  AL  WETRR DF VM+ HH++T  LI  SY+ RF +IGSVILALH+ SDVF
Sbjct: 131 GGLNVYAFFALFFWETRRSDFKVMLVHHIVTSFLIILSYVFRFARIGSVILALHEISDVF 190

Query: 186 MEAAKVFKYSEKELGASVFFGFFAISWLVLRLIFFPFWVIKATSYDLC---------AYL 245
           +E  K+ KYS  E   SV F  F +SW  LRLI++PFW++ +TSY+            +L
Sbjct: 191 LEIGKMCKYSGAETMTSVSFVLFFLSWTTLRLIYYPFWILWSTSYESIKVKTEYWDKKHL 250

Query: 246 KLSDVNSRLIYYVFNTMLLMLLVFHIYWWLLICSMISRQLKNRGKVGEDIRSDSEDED 290
             +     L YYVFNT+L  L + HIYWW+LI  ++  Q++ +GKV +DIRSDSE ED
Sbjct: 251 METGPPLILFYYVFNTLLYCLQILHIYWWILIYRVLISQIRAKGKVAKDIRSDSEGED 307

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LJK39.5e-10567.27Ceramide synthase LOH2 OS=Arabidopsis thaliana OX=3702 GN=LOH2 PE=1 SV=1[more]
Q84QC01.1e-8960.22ASC1-like protein 3 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0264000 PE=... [more]
Q6NQI82.6e-6245.05Ceramide synthase 1 LOH3 OS=Arabidopsis thaliana OX=3702 GN=LOH3 PE=1 SV=2[more]
Q6EUN01.7e-6151.05ASC1-like protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0581300 PE=... [more]
Q9LDF21.4e-6051.07Ceramide synthase LOH1 OS=Arabidopsis thaliana OX=3702 GN=LOH1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0K3733.6e-15596.89TLC domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G073690 PE=4 SV... [more]
A0A1S3BZU31.4e-15496.54LAG1 longevity assurance homolog 2 OS=Cucumis melo OX=3656 GN=LOC103495235 PE=4 ... [more]
A0A6J1EMG21.8e-14389.90LAG1 longevity assurance homolog 2-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1GMN82.4e-14390.24LAG1 longevity assurance homolog 2-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1I6015.4e-14389.90LAG1 longevity assurance homolog 2-like OS=Cucurbita maxima OX=3661 GN=LOC111471... [more]
Match NameE-valueIdentityDescription
XP_004136982.11.89e-19896.89LAG1 longevity assurance homolog 2 [Cucumis sativus] >KGN43933.1 hypothetical pr... [more]
XP_008454946.11.09e-19796.54PREDICTED: LAG1 longevity assurance homolog 2 [Cucumis melo][more]
XP_038888555.17.28e-18992.68LAG1 longevity assurance homolog 2 [Benincasa hispida][more]
KAG6588659.11.57e-18390.24LAG1 longevity assurance-like 2, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022927943.14.50e-18389.90LAG1 longevity assurance homolog 2-like [Cucurbita moschata] >XP_023530121.1 LAG... [more]
Match NameE-valueIdentityDescription
AT3G19260.16.8e-10667.27LAG1 homologue 2 [more]
AT1G13580.21.9e-6345.05LAG1 longevity assurance homolog 3 [more]
AT1G13580.31.9e-6345.05LAG1 longevity assurance homolog 3 [more]
AT3G25540.11.0e-6151.07TRAM, LAG1 and CLN8 (TLC) lipid-sensing domain containing protein [more]
AT1G26200.13.0e-5341.61TRAM, LAG1 and CLN8 (TLC) lipid-sensing domain containing protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006634TRAM/LAG1/CLN8 homology domainSMARTSM00724lag1_27coord: 65..272
e-value: 3.5E-61
score: 219.3
IPR006634TRAM/LAG1/CLN8 homology domainPFAMPF03798TRAM_LAG1_CLN8coord: 66..264
e-value: 5.6E-38
score: 130.7
IPR006634TRAM/LAG1/CLN8 homology domainPROSITEPS50922TLCcoord: 65..272
score: 19.640087
IPR016439Sphingosine N-acyltransferase Lag1/Lac1-likePANTHERPTHR12560LONGEVITY ASSURANCE FACTOR 1 LAG1coord: 13..286
NoneNo IPR availablePANTHERPTHR12560:SF53BNAA01G26210D PROTEINcoord: 13..286

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy1G000510.1Chy1G000510.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046513 ceramide biosynthetic process
biological_process GO:0042759 long-chain fatty acid biosynthetic process
biological_process GO:0002238 response to molecule of fungal origin
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0005794 Golgi apparatus
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0050291 sphingosine N-acyltransferase activity