Chy1G000110 (gene) Cucumber (hystrix) v1

Overview
NameChy1G000110
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
Descriptionallene oxide synthase
LocationchrH01: 88100 .. 89619 (-)
RNA-Seq ExpressionChy1G000110
SyntenyChy1G000110
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCTTCCTCCCCTGAACTTCCTCTCAAACCCATTCCCGGTGGCTATGGTTTCCCCTTCCTCGGTCCCATCAAAGACCGTTACGATTACTTCTATTTCCAAGGTAGAGACGAATTCTTCCGTTCCCGAATTACCAAATACAACTCCACCGTCTTCCGCGCCAATATGCCACCGGGCCCCTTCATCTCCTTCGATTCCAGAGTCGTTGTCCTCCTCGATGCCCTCAGTTTTCCCATTCTCTTCGACACCATCAAAGTCGAGAAACGCAACATTCTCGACGGAACTTACATGCCCTCCTTGTCCTTCACCGGCGGCATTCGCACCTGTGCTTATTTGGACCCGTCGGAAACAGAGCACTCTGTTCTTAAACGCCTCTTCCTCTCCTTTCTCGCTTCTCGCCATGACAGGTTCATCCCTCTGTTTCGAAGCTCCTTGTCTGAGATGTTTGTTAAGCTTGAAGATAAACTCGCCGACAAAAATAAGATCGCTGATTTCAACTCGATTAGTGATGCCATGTCGTTTGATTATGTTTTCCGTTTACTCTCCGATGGAACCCCTGATTCGAAATTAGCTGCTGATGGACCTGGAATGTTCGATTTATGGCTTGGGCTTCAACTCGCCCCATTGGCTTCCATTGGCCTTCCTAAAATTTTCTCTGTTTTTGAAGATCTCATTATTCACACCATTCCCCTGCCCTTCTTCCCAGTCAAGAGTGGTTACAGGTAGAGCGAGATTCAGAAACTTCTTTTAGATCCTGAAATGTTTATGAATTTTTTAATTTCATTTGTTGAATTTGGTTTGACAGGAAGCTTTATAAAGCGTTTTACTCCTCCTCTGGCTCATTTCTAGACGAAGCAGAGAAACAAGGGATAGACAGAGAGAAAGCATGTCACAATTTAGTGTTTCTTGCTGGATTCAACGCATACGGGGGAATGAAAATCCTTTTTCCCACTATACTGAAATGGGTGGGCACCGCTGGCGAGGATATCCACCGTAAACTGGCCGAGGAAGTGAGGGCCACCGTGAAGGAAGAAGGGGGACTGACTTTCTCCGCCTTGGAGAAAATGAGTCTGCTGAAGTCGGTCGTGTACGAAGCTCTGAGGATCGAACCGCCGGTGCCGTTCCAGTACGGGAAAGCGAAGGAGGATATCGTGATTCAGAGCCACGATTCTTCTTTCAAGATCAAAAAAGGGGAGACGATTTTTGGTTATCAGCCGTTTGCTACTAAAGATCCGAAGATTTTTAAGGACTCGGAGAAGTTCGTGGGCGATAGGTTCGTGGGAGAAGAAGGGGAGAAGCTTTTGAAGTATGTTTACTGGTCAAATGAGCGGGAGACGGTGGAGCCGACGGCGGAGAACAAGCAGTGTCCGGGGAAGAATCTGGTGGTGCTGATGGGCAGGATTATTGTGGTGGAATTCTTCCTCCGTTATGATACATTCATTGTGGACGTCGCAGATTTGGCGCTGGGTCCGGCGGTGAAGTTCAAGTCGTTAACCAGAGCCACCGCTGCGGTTTAG

mRNA sequence

ATGGCTTCTTCCTCCCCTGAACTTCCTCTCAAACCCATTCCCGGTGGCTATGGTTTCCCCTTCCTCGGTCCCATCAAAGACCGTTACGATTACTTCTATTTCCAAGGTAGAGACGAATTCTTCCGTTCCCGAATTACCAAATACAACTCCACCGTCTTCCGCGCCAATATGCCACCGGGCCCCTTCATCTCCTTCGATTCCAGAGTCGTTGTCCTCCTCGATGCCCTCAGTTTTCCCATTCTCTTCGACACCATCAAAGTCGAGAAACGCAACATTCTCGACGGAACTTACATGCCCTCCTTGTCCTTCACCGGCGGCATTCGCACCTGTGCTTATTTGGACCCGTCGGAAACAGAGCACTCTGTTCTTAAACGCCTCTTCCTCTCCTTTCTCGCTTCTCGCCATGACAGGTTCATCCCTCTGTTTCGAAGCTCCTTGTCTGAGATGTTTGTTAAGCTTGAAGATAAACTCGCCGACAAAAATAAGATCGCTGATTTCAACTCGATTAGTGATGCCATGTCGTTTGATTATGTTTTCCGTTTACTCTCCGATGGAACCCCTGATTCGAAATTAGCTGCTGATGGACCTGGAATGTTCGATTTATGGCTTGGGCTTCAACTCGCCCCATTGGCTTCCATTGGCCTTCCTAAAATTTTCTCTGTTTTTGAAGATCTCATTATTCACACCATTCCCCTGCCCTTCTTCCCAGTCAAGAGTGGTTACAGGAAGCTTTATAAAGCGTTTTACTCCTCCTCTGGCTCATTTCTAGACGAAGCAGAGAAACAAGGGATAGACAGAGAGAAAGCATGTCACAATTTAGTGTTTCTTGCTGGATTCAACGCATACGGGGGAATGAAAATCCTTTTTCCCACTATACTGAAATGGGTGGGCACCGCTGGCGAGGATATCCACCGTAAACTGGCCGAGGAAGTGAGGGCCACCGTGAAGGAAGAAGGGGGACTGACTTTCTCCGCCTTGGAGAAAATGAGTCTGCTGAAGTCGGTCGTGTACGAAGCTCTGAGGATCGAACCGCCGGTGCCGTTCCAGTACGGGAAAGCGAAGGAGGATATCGTGATTCAGAGCCACGATTCTTCTTTCAAGATCAAAAAAGGGGAGACGATTTTTGGTTATCAGCCGTTTGCTACTAAAGATCCGAAGATTTTTAAGGACTCGGAGAAGTTCGTGGGCGATAGGTTCGTGGGAGAAGAAGGGGAGAAGCTTTTGAAGTATGTTTACTGGTCAAATGAGCGGGAGACGGTGGAGCCGACGGCGGAGAACAAGCAGTGTCCGGGGAAGAATCTGGTGGTGCTGATGGGCAGGATTATTGTGGTGGAATTCTTCCTCCGTTATGATACATTCATTGTGGACGTCGCAGATTTGGCGCTGGGTCCGGCGGTGAAGTTCAAGTCGTTAACCAGAGCCACCGCTGCGGTTTAG

Coding sequence (CDS)

ATGGCTTCTTCCTCCCCTGAACTTCCTCTCAAACCCATTCCCGGTGGCTATGGTTTCCCCTTCCTCGGTCCCATCAAAGACCGTTACGATTACTTCTATTTCCAAGGTAGAGACGAATTCTTCCGTTCCCGAATTACCAAATACAACTCCACCGTCTTCCGCGCCAATATGCCACCGGGCCCCTTCATCTCCTTCGATTCCAGAGTCGTTGTCCTCCTCGATGCCCTCAGTTTTCCCATTCTCTTCGACACCATCAAAGTCGAGAAACGCAACATTCTCGACGGAACTTACATGCCCTCCTTGTCCTTCACCGGCGGCATTCGCACCTGTGCTTATTTGGACCCGTCGGAAACAGAGCACTCTGTTCTTAAACGCCTCTTCCTCTCCTTTCTCGCTTCTCGCCATGACAGGTTCATCCCTCTGTTTCGAAGCTCCTTGTCTGAGATGTTTGTTAAGCTTGAAGATAAACTCGCCGACAAAAATAAGATCGCTGATTTCAACTCGATTAGTGATGCCATGTCGTTTGATTATGTTTTCCGTTTACTCTCCGATGGAACCCCTGATTCGAAATTAGCTGCTGATGGACCTGGAATGTTCGATTTATGGCTTGGGCTTCAACTCGCCCCATTGGCTTCCATTGGCCTTCCTAAAATTTTCTCTGTTTTTGAAGATCTCATTATTCACACCATTCCCCTGCCCTTCTTCCCAGTCAAGAGTGGTTACAGGAAGCTTTATAAAGCGTTTTACTCCTCCTCTGGCTCATTTCTAGACGAAGCAGAGAAACAAGGGATAGACAGAGAGAAAGCATGTCACAATTTAGTGTTTCTTGCTGGATTCAACGCATACGGGGGAATGAAAATCCTTTTTCCCACTATACTGAAATGGGTGGGCACCGCTGGCGAGGATATCCACCGTAAACTGGCCGAGGAAGTGAGGGCCACCGTGAAGGAAGAAGGGGGACTGACTTTCTCCGCCTTGGAGAAAATGAGTCTGCTGAAGTCGGTCGTGTACGAAGCTCTGAGGATCGAACCGCCGGTGCCGTTCCAGTACGGGAAAGCGAAGGAGGATATCGTGATTCAGAGCCACGATTCTTCTTTCAAGATCAAAAAAGGGGAGACGATTTTTGGTTATCAGCCGTTTGCTACTAAAGATCCGAAGATTTTTAAGGACTCGGAGAAGTTCGTGGGCGATAGGTTCGTGGGAGAAGAAGGGGAGAAGCTTTTGAAGTATGTTTACTGGTCAAATGAGCGGGAGACGGTGGAGCCGACGGCGGAGAACAAGCAGTGTCCGGGGAAGAATCTGGTGGTGCTGATGGGCAGGATTATTGTGGTGGAATTCTTCCTCCGTTATGATACATTCATTGTGGACGTCGCAGATTTGGCGCTGGGTCCGGCGGTGAAGTTCAAGTCGTTAACCAGAGCCACCGCTGCGGTTTAG

Protein sequence

MASSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVFRANMPPGPFISFDSRVVVLLDALSFPILFDTIKVEKRNILDGTYMPSLSFTGGIRTCAYLDPSETEHSVLKRLFLSFLASRHDRFIPLFRSSLSEMFVKLEDKLADKNKIADFNSISDAMSFDYVFRLLSDGTPDSKLAADGPGMFDLWLGLQLAPLASIGLPKIFSVFEDLIIHTIPLPFFPVKSGYRKLYKAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKILFPTILKWVGTAGEDIHRKLAEEVRATVKEEGGLTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQSHDSSFKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETVEPTAENKQCPGKNLVVLMGRIIVVEFFLRYDTFIVDVADLALGPAVKFKSLTRATAAV*
Homology
BLAST of Chy1G000110 vs. ExPASy Swiss-Prot
Match: Q8GZP5 (Allene oxide synthase 3 OS=Solanum lycopersicum OX=4081 GN=AOS3 PE=1 SV=1)

HSP 1 Score: 564.3 bits (1453), Expect = 1.3e-159
Identity = 271/476 (56.93%), Postives = 361/476 (75.84%), Query Frame = 0

Query: 3   SSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVFRANMPPGPF 62
           SS P LP+K IPG YG PF G IKDRYD+ Y QG DEFFRSR+ KY+STVFR N+PPGPF
Sbjct: 18  SSIPSLPMKEIPGDYGVPFFGAIKDRYDFHYNQGADEFFRSRMKKYDSTVFRTNVPPGPF 77

Query: 63  ISFDSRVVVLLDALSFPILFDTIKVEKRNILDGTYMPSLSFTGGIRTCAYLDPSETEHSV 122
            + +S+VVVL+DA+S+PILFD  +V+K N  +GT+M S SF GG + C +L  S+ +H+ 
Sbjct: 78  NARNSKVVVLVDAVSYPILFDNSQVDKENYFEGTFMSSPSFNGGYKVCGFLGTSDPKHTT 137

Query: 123 LKRLFLSFLASRHDRFIPLFRSSLSEMFVKLEDKLADKNKIADFNSISDAMSFDYVFRLL 182
           LK LFLS L   HD+FIP+F +S++ MF  LE +L++K   + FN I D +SF+++FRL 
Sbjct: 138 LKGLFLSTLTRLHDKFIPIFTTSITSMFTSLEKELSEKG-TSYFNPIGDNLSFEFLFRLF 197

Query: 183 SDGTP--DSKLAADGPGMFDLWLGLQLAPLASIGLPKIFSVFEDLIIHTIPLPFFPVKSG 242
            +G    D+ +  +GP + D W+ LQLAPL S+GL  + +  EDL++HT PLP+  VK  
Sbjct: 198 CEGKNPIDTSVGPNGPKIVDKWVFLQLAPLISLGLKFVPNFLEDLVLHTFPLPYILVKRD 257

Query: 243 YRKLYKAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKILFPTILKWVGTAG 302
           ++KLY AFY+S    LDEAEK G+ R++ACHN VFLAGFN+YGG+K+ FP+++KW+GT+G
Sbjct: 258 HQKLYNAFYNSMKDILDEAEKLGVKRDEACHNFVFLAGFNSYGGLKVFFPSLIKWIGTSG 317

Query: 303 EDIHRKLAEEVRATVKEEGGLTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQ 362
             +H +L +E+R  VKE GG+T SA++KM L+KSVVYE LR++PPVPFQ  KA+++I+I 
Sbjct: 318 PSLHARLVKEIRTAVKEAGGVTLSAIDKMPLVKSVVYETLRMDPPVPFQTVKARKNIIIT 377

Query: 363 SHDSSFKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETV 422
           +H+SSF IKK E IFGYQP ATKD K+FK++E+F  DRFVG  GEKLLKYVYWSN +E  
Sbjct: 378 NHESSFLIKKDELIFGYQPLATKDSKVFKNAEEFNPDRFVG-GGEKLLKYVYWSNGKEID 437

Query: 423 EPTAENKQCPGKNLVVLMGRIIVVEFFLRYDTFIVDVADLALGPAVKFKSLTRATA 477
            P+  +KQCPGK+L+VLMGR++VVEFF+RYDTF V+   L LG  V FKSLT+AT+
Sbjct: 438 NPSVNDKQCPGKDLIVLMGRLLVVEFFMRYDTFEVEFGKLLLGSKVTFKSLTKATS 491

BLAST of Chy1G000110 vs. ExPASy Swiss-Prot
Match: K4BV52 (Allene oxide synthase 1, chloroplastic OS=Solanum lycopersicum OX=4081 GN=AOS1 PE=2 SV=1)

HSP 1 Score: 526.6 bits (1355), Expect = 3.0e-148
Identity = 265/481 (55.09%), Postives = 346/481 (71.93%), Query Frame = 0

Query: 2   ASSSP-----ELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVFRAN 61
           +SSSP     +LP + +PG YG P +GP KDR DYFY QG++EFF+SRI K+ STVFR N
Sbjct: 57  SSSSPPVKQAKLPAQKVPGDYGLPLVGPWKDRLDYFYNQGKNEFFKSRIQKHQSTVFRTN 116

Query: 62  MPPGPFISFDSRVVVLLDALSFPILFDTIKVEKRNILDGTYMPSLSFTGGIRTCAYLDPS 121
           MPPGPFISF+  VVVLLD  SFP+LFD  KVEK+++  GT+MPS   TGG R  +YLDPS
Sbjct: 117 MPPGPFISFNPNVVVLLDGKSFPVLFDVSKVEKKDLFTGTFMPSTDLTGGYRVLSYLDPS 176

Query: 122 ETEHSVLKRLFLSFLASRHDRFIPLFRSSLSEMFVKLEDKLADKNKIADFNSISDAMSFD 181
           E  H+ LK+L    L+SR +  IP F +S SE+F  LE++L+ K K A  N+ +D  +F+
Sbjct: 177 EPNHAKLKKLMFYLLSSRRNEVIPEFHNSYSELFETLENELSTKGK-AGLNAANDQAAFN 236

Query: 182 YVFRLLSDGTP-DSKLAADGPGMFDLWLGLQLAPLASIGLPKIFSVFEDLIIHTIPLPFF 241
           ++ R L    P D++L  DGP +   W+  QL PL  +GLPK   V EDL++HT  LP  
Sbjct: 237 FLARSLYGINPQDTELGTDGPKLIGKWVLFQLHPLLILGLPK---VLEDLVMHTFRLPPA 296

Query: 242 PVKSGYRKLYKAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKILFPTILKW 301
            VK  Y++LY  FY +S S LDEAEK GI RE+ACHNL+F   FN++GG+KI FP +LKW
Sbjct: 297 LVKKDYQRLYNFFYENSTSVLDEAEKIGISREEACHNLLFATCFNSFGGIKIFFPNMLKW 356

Query: 302 VGTAGEDIHRKLAEEVRATVKEEGG-LTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAK 361
           +G AG  +H +LA+E+R+ +    G +T +A+EKM L+KSVVYE+LRIEPPV  QYG+AK
Sbjct: 357 IGRAGAKLHSQLAQEIRSVISSNSGKVTMAAMEKMPLMKSVVYESLRIEPPVASQYGRAK 416

Query: 362 EDIVIQSHDSSFKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWS 421
            D+VI+SHD+SF+IK+GE ++GYQPFATKDPKIF  SE+FV DRF+GEEGEKLLK+V WS
Sbjct: 417 HDMVIESHDASFEIKEGELLYGYQPFATKDPKIFDRSEEFVADRFIGEEGEKLLKHVLWS 476

Query: 422 NERETVEPTAENKQCPGKNLVVLMGRIIVVEFFLRYDTFIVDVADLALGPAVKFKSLTRA 476
           N  ET   +  NKQC GK+ VVL+ R+++VE FLRYD+F ++V    LG A+   SL RA
Sbjct: 477 NGSETENASINNKQCAGKDFVVLVSRLLLVELFLRYDSFEIEVGASPLGAAITLTSLRRA 533

BLAST of Chy1G000110 vs. ExPASy Swiss-Prot
Match: Q96242 (Allene oxide synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CYP74A PE=1 SV=3)

HSP 1 Score: 512.3 bits (1318), Expect = 5.9e-144
Identity = 257/475 (54.11%), Postives = 335/475 (70.53%), Query Frame = 0

Query: 3   SSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVFRANMPPGPF 62
           + S +LP++ IPG YG P +GPIKDR+DYFY QG +EFF+SRI KYNSTV+R NMPPG F
Sbjct: 47  TGSKDLPIRNIPGNYGLPIVGPIKDRWDYFYDQGAEEFFKSRIRKYNSTVYRVNMPPGAF 106

Query: 63  ISFDSRVVVLLDALSFPILFDTIKVEKRNILDGTYMPSLSFTGGIRTCAYLDPSETEHSV 122
           I+ + +VV LLD  SFP+LFD  KVEK+++  GTYMPS   TGG R  +YLDPSE +H  
Sbjct: 107 IAENPQVVALLDGKSFPVLFDVDKVEKKDLFTGTYMPSTELTGGYRILSYLDPSEPKHEK 166

Query: 123 LKRLFLSFLASRHDRFIPLFRSSLSEMFVKLEDKLADKNKIADFNSISDAMSFDYVFRLL 182
           LK L    L S  +R  P F+++ SE+F  LE +L+ K K ADF   SD  +F+++ R  
Sbjct: 167 LKNLLFFLLKSSRNRIFPEFQATYSELFDSLEKELSLKGK-ADFGGSSDGTAFNFLARAF 226

Query: 183 SDGTP-DSKLAADGPGMFDLWLGLQLAPLASIGLPKIFSVFEDLIIHTIPLPFFPVKSGY 242
               P D+KL AD PG+   W+   L PL SIGLP+   V E+ +IHT  LP   VKS Y
Sbjct: 227 YGTNPADTKLKADAPGLITKWVLFNLHPLLSIGLPR---VIEEPLIHTFSLPPALVKSDY 286

Query: 243 RKLYKAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKILFPTILKWVGTAGE 302
           ++LY+ F  S+G  L EA+K GI RE+A HNL+F   FN +GGMKILFP ++K +G AG 
Sbjct: 287 QRLYEFFLESAGEILVEADKLGISREEATHNLLFATCFNTWGGMKILFPNMVKRIGRAGH 346

Query: 303 DIHRKLAEEVRATVKEEGG-LTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQ 362
            +H +LAEE+R+ +K  GG LT  A+EKM L KSVVYE LR EPPV  QYG+AK+D+VI+
Sbjct: 347 QVHNRLAEEIRSVIKSNGGELTMGAIEKMELTKSVVYECLRFEPPVTAQYGRAKKDLVIE 406

Query: 363 SHDSSFKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETV 422
           SHD++FK+K GE ++GYQP AT+DPKIF  +++FV +RFVGEEGEKLL++V WSN  ET 
Sbjct: 407 SHDAAFKVKAGEMLYGYQPLATRDPKIFDRADEFVPERFVGEEGEKLLRHVLWSNGPETE 466

Query: 423 EPTAENKQCPGKNLVVLMGRIIVVEFFLRYDTFIVDVADLALGPAVKFKSLTRAT 476
            PT  NKQC GK+ VVL+ R+ V+E F RYD+F ++V    LG +V F SL +A+
Sbjct: 467 TPTVGNKQCAGKDFVVLVARLFVIEIFRRYDSFDIEVGTSPLGSSVNFSSLRKAS 517

BLAST of Chy1G000110 vs. ExPASy Swiss-Prot
Match: Q7XYS3 (Allene oxide synthase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP74A2 PE=2 SV=2)

HSP 1 Score: 507.7 bits (1306), Expect = 1.5e-142
Identity = 251/474 (52.95%), Postives = 333/474 (70.25%), Query Frame = 0

Query: 8   LPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVFRANMPPGPFISFDS 67
           LP +P+PG YG PF+  ++DR D++Y QG+D++F SR  +Y STV R N+PPGPF++ D 
Sbjct: 7   LPRRPVPGSYGVPFVSAVRDRLDFYYLQGQDKYFESRAERYGSTVVRINVPPGPFMARDP 66

Query: 68  RVVVLLDALSFPILFDTIKVEKRNILDGTYMPSLSFTGGIRTCAYLDPSETEHSVLKRLF 127
           RVV LLDA SFP+LFD  KVEKR++  GT+MPS S TGG R CAYLDPSE  H+ +K+L 
Sbjct: 67  RVVALLDAKSFPVLFDVAKVEKRDVFTGTFMPSTSLTGGYRVCAYLDPSEPNHAKIKQLL 126

Query: 128 LSFLASRHDRFIPLFRSSLSEMFVKLEDKLADKNKIADFNSISDAMSFDYVFRLLSDGTP 187
           LS L SR D F+P+FRS+   +   +E +LA     +DF +++DA SF+++        P
Sbjct: 127 LSLLVSRKDAFVPVFRSNFGALLDTVESQLASGGGKSDFTALNDATSFEFIGEAYFGVRP 186

Query: 188 --DSKLAADGPGMFDLWLGLQLAPLASIGLPKIFSVFEDLIIHTIPLPFFPVKSGYRKLY 247
              S L   GP    LWL  QLAPL ++GLP I    ED ++HT+PLP F + S Y+ LY
Sbjct: 187 SASSSLGTGGPTKAALWLLWQLAPLTTLGLPMI---IEDPLLHTLPLPPFLISSDYKALY 246

Query: 248 KAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKILFPTILKWVGTAGEDIHR 307
             F +++   LD AE  G+ RE+ACHNL+F   FN+YGG K+L P IL  V  AGE +H 
Sbjct: 247 AYFAAAASQALDAAEGLGLSREEACHNLLFATVFNSYGGFKLLLPQILSRVAQAGEKLHE 306

Query: 308 KLAEEVRATVKEEGG-LTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQSHDS 367
           +LA E+R+ V + GG +T +ALEKM L +SVV+EALR++PPV FQYG+AK D+ I+SHD+
Sbjct: 307 RLAAEIRSAVADAGGNVTLAALEKMELTRSVVWEALRLDPPVRFQYGRAKADLEIESHDA 366

Query: 368 SFKIKKGETIFGYQPFATKDPKIF-KDSEKFVGDRFVGEEGEKLLKYVYWSNERETVEPT 427
           SF IKKGE +FGYQP AT+DP++F   + +FVGDRFVGEEG KLL+YVYWSN RET  P+
Sbjct: 367 SFAIKKGEMLFGYQPCATRDPRVFGATAREFVGDRFVGEEGRKLLQYVYWSNGRETENPS 426

Query: 428 AENKQCPGKNLVVLMGRIIVVEFFLRYDTFIVDVADLALGPAVKFKSLTRATAA 478
            +NKQCPGKNLVVL+GR+++VE FLRYDTF  +      G  V    +T+A+ +
Sbjct: 427 VDNKQCPGKNLVVLVGRLLLVELFLRYDTFTAEA-----GKKVVITGVTKASTS 472

BLAST of Chy1G000110 vs. ExPASy Swiss-Prot
Match: Q9LLB0 (Allene oxide synthase 2, chloroplastic OS=Solanum lycopersicum OX=4081 GN=AOS2 PE=1 SV=1)

HSP 1 Score: 503.8 bits (1296), Expect = 2.1e-141
Identity = 249/470 (52.98%), Postives = 331/470 (70.43%), Query Frame = 0

Query: 7   ELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVFRANMPPGPFISFD 66
           +L  + IPG YG P +GP KDR DYFY QG+++FF SRI KY ST+FR NMPPGPFI+ +
Sbjct: 45  KLSTRTIPGDYGLPGIGPWKDRLDYFYNQGKNDFFESRIAKYKSTIFRTNMPPGPFITSN 104

Query: 67  SRVVVLLDALSFPILFDTIKVEKRNILDGTYMPSLSFTGGIRTCAYLDPSETEHSVLKRL 126
            +V+VLLD  SFP+LFD  KVEK+++  GT++PS   TGG R  +YLDPSE  H  LK+L
Sbjct: 105 PKVIVLLDGKSFPVLFDASKVEKKDLFTGTFVPSTELTGGYRILSYLDPSEPNHEKLKKL 164

Query: 127 FLSFLASRHDRFIPLFRSSLSEMFVKLEDKLADKNKIADFNSISDAMSFDYVFRLLSDGT 186
               L+SR D  IP F  + +E+F  L+ ++ +K  +  FNS SD  +F+++ R L    
Sbjct: 165 MFFLLSSRRDHVIPEFHETYTELFETLDKEMEEKGTVG-FNSGSDQAAFNFLARSLFGVN 224

Query: 187 P-DSKLAADGPGMFDLWLGLQLAPLASIGLPKIFSVFEDLIIHTIPLPFFPVKSGYRKLY 246
           P ++KL  DGP +   W+ LQL P+ ++GLPK     +D+++HT  LP   VK  Y++LY
Sbjct: 225 PVETKLGTDGPALIGKWILLQLHPVITLGLPKF---LDDVLLHTFRLPPILVKKDYQRLY 284

Query: 247 KAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKILFPTILKWVGTAGEDIHR 306
             FY++S +   EAEK GI +++ACHNL+F   FN++GGMKI FP +LK +  AG +IH 
Sbjct: 285 DFFYTNSANLFIEAEKLGISKDEACHNLLFATCFNSFGGMKIFFPNMLKSIAKAGVEIHT 344

Query: 307 KLAEEVRATVKEEGG-LTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQSHDS 366
           +LA E+R+ VK  GG +T SA+EKM L+KSVVYEALR++PPV  QYG+AK+D+ I+SHD+
Sbjct: 345 RLANEIRSEVKSAGGKITMSAMEKMPLMKSVVYEALRVDPPVASQYGRAKQDLKIESHDA 404

Query: 367 SFKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETVEPTA 426
            F++KKGE +FGYQPFATKDPKIF    +FV DRFVGEEGEKLLK+V WSN  ET  PT 
Sbjct: 405 VFEVKKGEILFGYQPFATKDPKIFDRPGEFVADRFVGEEGEKLLKHVLWSNGPETESPTV 464

Query: 427 ENKQCPGKNLVVLMGRIIVVEFFLRYDTFIVDVADLALGPAVKFKSLTRA 475
            NKQC GK+ VV++ R+ V EFFLRY T  VDV   ALG ++   SL +A
Sbjct: 465 GNKQCAGKDFVVMVSRLFVTEFFLRYGTLNVDVGTSALGSSITITSLKKA 510

BLAST of Chy1G000110 vs. ExPASy TrEMBL
Match: U5YTM6 (Hydroperoxide lyase OS=Cucumis sativus OX=3659 GN=HPL PE=2 SV=1)

HSP 1 Score: 931.0 bits (2405), Expect = 2.0e-267
Identity = 463/478 (96.86%), Postives = 468/478 (97.91%), Query Frame = 0

Query: 1   MASSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVFRANMPPG 60
           MASSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVF ANMPPG
Sbjct: 1   MASSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVFHANMPPG 60

Query: 61  PFISFDSRVVVLLDALSFPILFDTIKVEKRNILDGTYMPSLSFTGGIRTCAYLDPSETEH 120
           PFIS DSRVVVLLDALSFPILFDT KVEKRNILDGTYMPSLSFTGGIRTCAYLDPSETEH
Sbjct: 61  PFISSDSRVVVLLDALSFPILFDTTKVEKRNILDGTYMPSLSFTGGIRTCAYLDPSETEH 120

Query: 121 SVLKRLFLSFLASRHDRFIPLFRSSLSEMFVKLEDKLADKNKIADFNSISDAMSFDYVFR 180
           +VLKRLFLSFLASRHDRFIPLFRSSLSEMFVKLEDKLADKNKIADFNSISDAMSFDYVFR
Sbjct: 121 TVLKRLFLSFLASRHDRFIPLFRSSLSEMFVKLEDKLADKNKIADFNSISDAMSFDYVFR 180

Query: 181 LLSDGTPDSKLAADGPGMFDLWLGLQLAPLASIGLPKIFSVFEDLIIHTIPLPFFPVKSG 240
           L SDGTPDS LAADGPGMFDLWLGLQLAPLASIGLPKIFSVFEDLIIHTIPLPFFPVKS 
Sbjct: 181 LFSDGTPDSTLAADGPGMFDLWLGLQLAPLASIGLPKIFSVFEDLIIHTIPLPFFPVKSR 240

Query: 241 YRKLYKAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKILFPTILKWVGTAG 300
           YRKLYKAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMK+LFPTILKWVGT G
Sbjct: 241 YRKLYKAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPTILKWVGTGG 300

Query: 301 EDIHRKLAEEVRATVKEEGGLTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQ 360
           ED+HRKLAEEVR TVKEEGGLTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQ
Sbjct: 301 EDLHRKLAEEVRTTVKEEGGLTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQ 360

Query: 361 SHDSSFKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETV 420
           SHDS FKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETV
Sbjct: 361 SHDSCFKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETV 420

Query: 421 EPTAENKQCPGKNLVVLMGRIIVVEFFLRYDTFIVDVADLALGPAVKFKSLTRATAAV 479
           EPTAENKQCPGKNLVV+MGRIIVVEFFLRYDTF VDVADLALGPAVKFKSLTRATA+V
Sbjct: 421 EPTAENKQCPGKNLVVMMGRIIVVEFFLRYDTFTVDVADLALGPAVKFKSLTRATASV 478

BLAST of Chy1G000110 vs. ExPASy TrEMBL
Match: Q9M5J2 (Fatty acid hydroperoxide lyase OS=Cucumis sativus OX=3659 PE=2 SV=1)

HSP 1 Score: 927.5 bits (2396), Expect = 2.2e-266
Identity = 461/478 (96.44%), Postives = 467/478 (97.70%), Query Frame = 0

Query: 1   MASSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVFRANMPPG 60
           MASSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVF ANMPPG
Sbjct: 1   MASSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVFHANMPPG 60

Query: 61  PFISFDSRVVVLLDALSFPILFDTIKVEKRNILDGTYMPSLSFTGGIRTCAYLDPSETEH 120
           PFIS DSRVVVLLDALSFPILFDT KVEKRNILDGTYMPSLSFTGGIRTCAYLDPSETEH
Sbjct: 61  PFISSDSRVVVLLDALSFPILFDTTKVEKRNILDGTYMPSLSFTGGIRTCAYLDPSETEH 120

Query: 121 SVLKRLFLSFLASRHDRFIPLFRSSLSEMFVKLEDKLADKNKIADFNSISDAMSFDYVFR 180
           +VLKRLFLSFLAS HDRFIPLFRSSLSEMFVKLEDKLADKNKIADFNSISDA+SFDYVFR
Sbjct: 121 TVLKRLFLSFLASHHDRFIPLFRSSLSEMFVKLEDKLADKNKIADFNSISDAVSFDYVFR 180

Query: 181 LLSDGTPDSKLAADGPGMFDLWLGLQLAPLASIGLPKIFSVFEDLIIHTIPLPFFPVKSG 240
           L SDGTPDS LAADGPGMFDLWLGLQLAPLASIGLPKIFSVFEDLIIHTIPLPFFPVKS 
Sbjct: 181 LFSDGTPDSTLAADGPGMFDLWLGLQLAPLASIGLPKIFSVFEDLIIHTIPLPFFPVKSR 240

Query: 241 YRKLYKAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKILFPTILKWVGTAG 300
           YRKLYKAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMK+LFPTILKWVGT G
Sbjct: 241 YRKLYKAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPTILKWVGTGG 300

Query: 301 EDIHRKLAEEVRATVKEEGGLTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQ 360
           ED+HRKLAEEVR TVKEEGGLTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQ
Sbjct: 301 EDLHRKLAEEVRTTVKEEGGLTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQ 360

Query: 361 SHDSSFKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETV 420
           SHDS FKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETV
Sbjct: 361 SHDSCFKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETV 420

Query: 421 EPTAENKQCPGKNLVVLMGRIIVVEFFLRYDTFIVDVADLALGPAVKFKSLTRATAAV 479
           EPTAENKQCPGKNLVV+MGRIIVVEFFLRYDTF VDVADLALGPAVKFKSLTRATA+V
Sbjct: 421 EPTAENKQCPGKNLVVMMGRIIVVEFFLRYDTFTVDVADLALGPAVKFKSLTRATASV 478

BLAST of Chy1G000110 vs. ExPASy TrEMBL
Match: A0A5D3C5U2 (Allene oxide synthase 2-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G00110 PE=4 SV=1)

HSP 1 Score: 915.2 bits (2364), Expect = 1.1e-262
Identity = 453/477 (94.97%), Postives = 466/477 (97.69%), Query Frame = 0

Query: 2   ASSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVFRANMPPGP 61
           +SSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVFRANMPPGP
Sbjct: 5   SSSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVFRANMPPGP 64

Query: 62  FISFDSRVVVLLDALSFPILFDTIKVEKRNILDGTYMPSLSFTGGIRTCAYLDPSETEHS 121
           FIS DSRVVVLLDALSFPILFDT KVEKRNILDGTYMPSLSFTG IRTCAYLDPSETEHS
Sbjct: 65  FISSDSRVVVLLDALSFPILFDTAKVEKRNILDGTYMPSLSFTGNIRTCAYLDPSETEHS 124

Query: 122 VLKRLFLSFLASRHDRFIPLFRSSLSEMFVKLEDKLADKNKIADFNSISDAMSFDYVFRL 181
           VLKRLFLSFLASRHDRFIPLFRSSLSEMFVKLEDKL++K KIADFNSISD+MSFDYVFRL
Sbjct: 125 VLKRLFLSFLASRHDRFIPLFRSSLSEMFVKLEDKLSEKKKIADFNSISDSMSFDYVFRL 184

Query: 182 LSDGTPDSKLAADGPGMFDLWLGLQLAPLASIGLPKIFSVFEDLIIHTIPLPFFPVKSGY 241
           LSDGTPDSKLAA+GPGMFDLWL  QLAPLASIGLPKIFSVFEDL+IHTIPLPFFPVKSGY
Sbjct: 185 LSDGTPDSKLAAEGPGMFDLWLVFQLAPLASIGLPKIFSVFEDLVIHTIPLPFFPVKSGY 244

Query: 242 RKLYKAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKILFPTILKWVGTAGE 301
           RKLY+AFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMK+LFPT+LKWVGTAGE
Sbjct: 245 RKLYEAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPTLLKWVGTAGE 304

Query: 302 DIHRKLAEEVRATVKEEGGLTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQS 361
           D+HRKLAEEVR TVKEEGGLTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQS
Sbjct: 305 DLHRKLAEEVRTTVKEEGGLTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQS 364

Query: 362 HDSSFKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETVE 421
           HDSSFKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETVE
Sbjct: 365 HDSSFKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETVE 424

Query: 422 PTAENKQCPGKNLVVLMGRIIVVEFFLRYDTFIVDVADLALGPAVKFKSLTRATAAV 479
           PTAENKQCPGKNLVVL+GRI+VVEFFLRYDTF V+VADL LGPAVKFKSLTRAT  V
Sbjct: 425 PTAENKQCPGKNLVVLIGRIMVVEFFLRYDTFTVEVADLPLGPAVKFKSLTRATDMV 481

BLAST of Chy1G000110 vs. ExPASy TrEMBL
Match: A0A1S3BZK9 (allene oxide synthase 2-like isoform X1 OS=Cucumis melo OX=3656 GN=HPL PE=4 SV=1)

HSP 1 Score: 915.2 bits (2364), Expect = 1.1e-262
Identity = 453/477 (94.97%), Postives = 466/477 (97.69%), Query Frame = 0

Query: 2   ASSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVFRANMPPGP 61
           +SSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVFRANMPPGP
Sbjct: 5   SSSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVFRANMPPGP 64

Query: 62  FISFDSRVVVLLDALSFPILFDTIKVEKRNILDGTYMPSLSFTGGIRTCAYLDPSETEHS 121
           FIS DSRVVVLLDALSFPILFDT KVEKRNILDGTYMPSLSFTG IRTCAYLDPSETEHS
Sbjct: 65  FISSDSRVVVLLDALSFPILFDTAKVEKRNILDGTYMPSLSFTGNIRTCAYLDPSETEHS 124

Query: 122 VLKRLFLSFLASRHDRFIPLFRSSLSEMFVKLEDKLADKNKIADFNSISDAMSFDYVFRL 181
           VLKRLFLSFLASRHDRFIPLFRSSLSEMFVKLEDKL++K KIADFNSISD+MSFDYVFRL
Sbjct: 125 VLKRLFLSFLASRHDRFIPLFRSSLSEMFVKLEDKLSEKKKIADFNSISDSMSFDYVFRL 184

Query: 182 LSDGTPDSKLAADGPGMFDLWLGLQLAPLASIGLPKIFSVFEDLIIHTIPLPFFPVKSGY 241
           LSDGTPDSKLAA+GPGMFDLWL  QLAPLASIGLPKIFSVFEDL+IHTIPLPFFPVKSGY
Sbjct: 185 LSDGTPDSKLAAEGPGMFDLWLVFQLAPLASIGLPKIFSVFEDLVIHTIPLPFFPVKSGY 244

Query: 242 RKLYKAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKILFPTILKWVGTAGE 301
           RKLY+AFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMK+LFPT+LKWVGTAGE
Sbjct: 245 RKLYEAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPTLLKWVGTAGE 304

Query: 302 DIHRKLAEEVRATVKEEGGLTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQS 361
           D+HRKLAEEVR TVKEEGGLTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQS
Sbjct: 305 DLHRKLAEEVRTTVKEEGGLTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQS 364

Query: 362 HDSSFKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETVE 421
           HDSSFKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETVE
Sbjct: 365 HDSSFKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETVE 424

Query: 422 PTAENKQCPGKNLVVLMGRIIVVEFFLRYDTFIVDVADLALGPAVKFKSLTRATAAV 479
           PTAENKQCPGKNLVVL+GRI+VVEFFLRYDTF V+VADL LGPAVKFKSLTRAT  V
Sbjct: 425 PTAENKQCPGKNLVVLIGRIMVVEFFLRYDTFTVEVADLPLGPAVKFKSLTRATDMV 481

BLAST of Chy1G000110 vs. ExPASy TrEMBL
Match: Q93XR3 (Fatty acid 9-hydroperoxide lyase OS=Cucumis melo OX=3656 GN=HPL PE=2 SV=1)

HSP 1 Score: 913.3 bits (2359), Expect = 4.2e-262
Identity = 452/477 (94.76%), Postives = 465/477 (97.48%), Query Frame = 0

Query: 2   ASSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVFRANMPPGP 61
           +SSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVFRANMPPGP
Sbjct: 5   SSSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVFRANMPPGP 64

Query: 62  FISFDSRVVVLLDALSFPILFDTIKVEKRNILDGTYMPSLSFTGGIRTCAYLDPSETEHS 121
           FIS DSRVVVLLDALSFPILFDT KVEKRNILDGTYMPSLSFTG IRTCAYLDPSETEHS
Sbjct: 65  FISSDSRVVVLLDALSFPILFDTAKVEKRNILDGTYMPSLSFTGNIRTCAYLDPSETEHS 124

Query: 122 VLKRLFLSFLASRHDRFIPLFRSSLSEMFVKLEDKLADKNKIADFNSISDAMSFDYVFRL 181
           VLKRLFLSFLASRHDRFIPLFRSSLSEMFVKLEDKL++K KIADFNSISD+MSFDYVFRL
Sbjct: 125 VLKRLFLSFLASRHDRFIPLFRSSLSEMFVKLEDKLSEKKKIADFNSISDSMSFDYVFRL 184

Query: 182 LSDGTPDSKLAADGPGMFDLWLGLQLAPLASIGLPKIFSVFEDLIIHTIPLPFFPVKSGY 241
           LSDGTPDSKLAA+GPGMFDLWL  QLAPLASIGLPKIFSVFEDL+IHTIPLPFFPVKSGY
Sbjct: 185 LSDGTPDSKLAAEGPGMFDLWLVFQLAPLASIGLPKIFSVFEDLVIHTIPLPFFPVKSGY 244

Query: 242 RKLYKAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKILFPTILKWVGTAGE 301
           RKLY+AFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMK+LFPT+LKWVGTAGE
Sbjct: 245 RKLYEAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPTLLKWVGTAGE 304

Query: 302 DIHRKLAEEVRATVKEEGGLTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQS 361
           D+HRKLAEEVR TVKEEGGLTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQS
Sbjct: 305 DLHRKLAEEVRTTVKEEGGLTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQS 364

Query: 362 HDSSFKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETVE 421
           HDSSFKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETVE
Sbjct: 365 HDSSFKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETVE 424

Query: 422 PTAENKQCPGKNLVVLMGRIIVVEFFLRYDTFIVDVADLALGPAVKFKSLTRATAAV 479
           PT ENKQCPGKNLVVL+GRI+VVEFFLRYDTF V+VADL LGPAVKFKSLTRAT  V
Sbjct: 425 PTPENKQCPGKNLVVLIGRIMVVEFFLRYDTFTVEVADLPLGPAVKFKSLTRATDMV 481

BLAST of Chy1G000110 vs. NCBI nr
Match: NP_001274399.1 (allene oxide synthase, chloroplastic-like [Cucumis sativus] >XP_011658536.1 allene oxide synthase, chloroplastic-like isoform X1 [Cucumis sativus] >XP_031744184.1 allene oxide synthase, chloroplastic-like isoform X1 [Cucumis sativus] >AGZ95025.1 hydroperoxide lyase [Cucumis sativus] >AHC08715.1 hydroperoxide lyase [Cucumis sativus] >KGN43979.1 hypothetical protein Csa_017689 [Cucumis sativus])

HSP 1 Score: 924 bits (2389), Expect = 0.0
Identity = 463/478 (96.86%), Postives = 468/478 (97.91%), Query Frame = 0

Query: 1   MASSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVFRANMPPG 60
           MASSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVF ANMPPG
Sbjct: 1   MASSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVFHANMPPG 60

Query: 61  PFISFDSRVVVLLDALSFPILFDTIKVEKRNILDGTYMPSLSFTGGIRTCAYLDPSETEH 120
           PFIS DSRVVVLLDALSFPILFDT KVEKRNILDGTYMPSLSFTGGIRTCAYLDPSETEH
Sbjct: 61  PFISSDSRVVVLLDALSFPILFDTTKVEKRNILDGTYMPSLSFTGGIRTCAYLDPSETEH 120

Query: 121 SVLKRLFLSFLASRHDRFIPLFRSSLSEMFVKLEDKLADKNKIADFNSISDAMSFDYVFR 180
           +VLKRLFLSFLASRHDRFIPLFRSSLSEMFVKLEDKLADKNKIADFNSISDAMSFDYVFR
Sbjct: 121 TVLKRLFLSFLASRHDRFIPLFRSSLSEMFVKLEDKLADKNKIADFNSISDAMSFDYVFR 180

Query: 181 LLSDGTPDSKLAADGPGMFDLWLGLQLAPLASIGLPKIFSVFEDLIIHTIPLPFFPVKSG 240
           L SDGTPDS LAADGPGMFDLWLGLQLAPLASIGLPKIFSVFEDLIIHTIPLPFFPVKS 
Sbjct: 181 LFSDGTPDSTLAADGPGMFDLWLGLQLAPLASIGLPKIFSVFEDLIIHTIPLPFFPVKSR 240

Query: 241 YRKLYKAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKILFPTILKWVGTAG 300
           YRKLYKAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMK+LFPTILKWVGT G
Sbjct: 241 YRKLYKAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPTILKWVGTGG 300

Query: 301 EDIHRKLAEEVRATVKEEGGLTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQ 360
           ED+HRKLAEEVR TVKEEGGLTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQ
Sbjct: 301 EDLHRKLAEEVRTTVKEEGGLTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQ 360

Query: 361 SHDSSFKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETV 420
           SHDS FKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETV
Sbjct: 361 SHDSCFKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETV 420

Query: 421 EPTAENKQCPGKNLVVLMGRIIVVEFFLRYDTFIVDVADLALGPAVKFKSLTRATAAV 478
           EPTAENKQCPGKNLVV+MGRIIVVEFFLRYDTF VDVADLALGPAVKFKSLTRATA+V
Sbjct: 421 EPTAENKQCPGKNLVVMMGRIIVVEFFLRYDTFTVDVADLALGPAVKFKSLTRATASV 478

BLAST of Chy1G000110 vs. NCBI nr
Match: AAF64041.1 (fatty acid hydroperoxide lyase [Cucumis sativus])

HSP 1 Score: 921 bits (2380), Expect = 0.0
Identity = 461/478 (96.44%), Postives = 467/478 (97.70%), Query Frame = 0

Query: 1   MASSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVFRANMPPG 60
           MASSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVF ANMPPG
Sbjct: 1   MASSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVFHANMPPG 60

Query: 61  PFISFDSRVVVLLDALSFPILFDTIKVEKRNILDGTYMPSLSFTGGIRTCAYLDPSETEH 120
           PFIS DSRVVVLLDALSFPILFDT KVEKRNILDGTYMPSLSFTGGIRTCAYLDPSETEH
Sbjct: 61  PFISSDSRVVVLLDALSFPILFDTTKVEKRNILDGTYMPSLSFTGGIRTCAYLDPSETEH 120

Query: 121 SVLKRLFLSFLASRHDRFIPLFRSSLSEMFVKLEDKLADKNKIADFNSISDAMSFDYVFR 180
           +VLKRLFLSFLAS HDRFIPLFRSSLSEMFVKLEDKLADKNKIADFNSISDA+SFDYVFR
Sbjct: 121 TVLKRLFLSFLASHHDRFIPLFRSSLSEMFVKLEDKLADKNKIADFNSISDAVSFDYVFR 180

Query: 181 LLSDGTPDSKLAADGPGMFDLWLGLQLAPLASIGLPKIFSVFEDLIIHTIPLPFFPVKSG 240
           L SDGTPDS LAADGPGMFDLWLGLQLAPLASIGLPKIFSVFEDLIIHTIPLPFFPVKS 
Sbjct: 181 LFSDGTPDSTLAADGPGMFDLWLGLQLAPLASIGLPKIFSVFEDLIIHTIPLPFFPVKSR 240

Query: 241 YRKLYKAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKILFPTILKWVGTAG 300
           YRKLYKAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMK+LFPTILKWVGT G
Sbjct: 241 YRKLYKAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPTILKWVGTGG 300

Query: 301 EDIHRKLAEEVRATVKEEGGLTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQ 360
           ED+HRKLAEEVR TVKEEGGLTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQ
Sbjct: 301 EDLHRKLAEEVRTTVKEEGGLTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQ 360

Query: 361 SHDSSFKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETV 420
           SHDS FKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETV
Sbjct: 361 SHDSCFKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETV 420

Query: 421 EPTAENKQCPGKNLVVLMGRIIVVEFFLRYDTFIVDVADLALGPAVKFKSLTRATAAV 478
           EPTAENKQCPGKNLVV+MGRIIVVEFFLRYDTF VDVADLALGPAVKFKSLTRATA+V
Sbjct: 421 EPTAENKQCPGKNLVVMMGRIIVVEFFLRYDTFTVDVADLALGPAVKFKSLTRATASV 478

BLAST of Chy1G000110 vs. NCBI nr
Match: XP_008454885.1 (PREDICTED: allene oxide synthase 2-like isoform X1 [Cucumis melo] >KAA0031259.1 allene oxide synthase 2-like isoform X1 [Cucumis melo var. makuwa] >TYK06710.1 allene oxide synthase 2-like isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 909 bits (2349), Expect = 0.0
Identity = 452/474 (95.36%), Postives = 465/474 (98.10%), Query Frame = 0

Query: 2   ASSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVFRANMPPGP 61
           +SSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVFRANMPPGP
Sbjct: 5   SSSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVFRANMPPGP 64

Query: 62  FISFDSRVVVLLDALSFPILFDTIKVEKRNILDGTYMPSLSFTGGIRTCAYLDPSETEHS 121
           FIS DSRVVVLLDALSFPILFDT KVEKRNILDGTYMPSLSFTG IRTCAYLDPSETEHS
Sbjct: 65  FISSDSRVVVLLDALSFPILFDTAKVEKRNILDGTYMPSLSFTGNIRTCAYLDPSETEHS 124

Query: 122 VLKRLFLSFLASRHDRFIPLFRSSLSEMFVKLEDKLADKNKIADFNSISDAMSFDYVFRL 181
           VLKRLFLSFLASRHDRFIPLFRSSLSEMFVKLEDKL++K KIADFNSISD+MSFDYVFRL
Sbjct: 125 VLKRLFLSFLASRHDRFIPLFRSSLSEMFVKLEDKLSEKKKIADFNSISDSMSFDYVFRL 184

Query: 182 LSDGTPDSKLAADGPGMFDLWLGLQLAPLASIGLPKIFSVFEDLIIHTIPLPFFPVKSGY 241
           LSDGTPDSKLAA+GPGMFDLWL  QLAPLASIGLPKIFSVFEDL+IHTIPLPFFPVKSGY
Sbjct: 185 LSDGTPDSKLAAEGPGMFDLWLVFQLAPLASIGLPKIFSVFEDLVIHTIPLPFFPVKSGY 244

Query: 242 RKLYKAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKILFPTILKWVGTAGE 301
           RKLY+AFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMK+LFPT+LKWVGTAGE
Sbjct: 245 RKLYEAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPTLLKWVGTAGE 304

Query: 302 DIHRKLAEEVRATVKEEGGLTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQS 361
           D+HRKLAEEVR TVKEEGGLTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQS
Sbjct: 305 DLHRKLAEEVRTTVKEEGGLTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQS 364

Query: 362 HDSSFKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETVE 421
           HDSSFKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETVE
Sbjct: 365 HDSSFKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETVE 424

Query: 422 PTAENKQCPGKNLVVLMGRIIVVEFFLRYDTFIVDVADLALGPAVKFKSLTRAT 475
           PTAENKQCPGKNLVVL+GRI+VVEFFLRYDTF V+VADL LGPAVKFKSLTRAT
Sbjct: 425 PTAENKQCPGKNLVVLIGRIMVVEFFLRYDTFTVEVADLPLGPAVKFKSLTRAT 478

BLAST of Chy1G000110 vs. NCBI nr
Match: NP_001284390.1 (allene oxide synthase 2-like [Cucumis melo] >AAK54282.1 fatty acid 9-hydroperoxide lyase [Cucumis melo])

HSP 1 Score: 907 bits (2344), Expect = 0.0
Identity = 451/474 (95.15%), Postives = 464/474 (97.89%), Query Frame = 0

Query: 2   ASSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVFRANMPPGP 61
           +SSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVFRANMPPGP
Sbjct: 5   SSSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVFRANMPPGP 64

Query: 62  FISFDSRVVVLLDALSFPILFDTIKVEKRNILDGTYMPSLSFTGGIRTCAYLDPSETEHS 121
           FIS DSRVVVLLDALSFPILFDT KVEKRNILDGTYMPSLSFTG IRTCAYLDPSETEHS
Sbjct: 65  FISSDSRVVVLLDALSFPILFDTAKVEKRNILDGTYMPSLSFTGNIRTCAYLDPSETEHS 124

Query: 122 VLKRLFLSFLASRHDRFIPLFRSSLSEMFVKLEDKLADKNKIADFNSISDAMSFDYVFRL 181
           VLKRLFLSFLASRHDRFIPLFRSSLSEMFVKLEDKL++K KIADFNSISD+MSFDYVFRL
Sbjct: 125 VLKRLFLSFLASRHDRFIPLFRSSLSEMFVKLEDKLSEKKKIADFNSISDSMSFDYVFRL 184

Query: 182 LSDGTPDSKLAADGPGMFDLWLGLQLAPLASIGLPKIFSVFEDLIIHTIPLPFFPVKSGY 241
           LSDGTPDSKLAA+GPGMFDLWL  QLAPLASIGLPKIFSVFEDL+IHTIPLPFFPVKSGY
Sbjct: 185 LSDGTPDSKLAAEGPGMFDLWLVFQLAPLASIGLPKIFSVFEDLVIHTIPLPFFPVKSGY 244

Query: 242 RKLYKAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKILFPTILKWVGTAGE 301
           RKLY+AFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMK+LFPT+LKWVGTAGE
Sbjct: 245 RKLYEAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPTLLKWVGTAGE 304

Query: 302 DIHRKLAEEVRATVKEEGGLTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQS 361
           D+HRKLAEEVR TVKEEGGLTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQS
Sbjct: 305 DLHRKLAEEVRTTVKEEGGLTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQS 364

Query: 362 HDSSFKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETVE 421
           HDSSFKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETVE
Sbjct: 365 HDSSFKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETVE 424

Query: 422 PTAENKQCPGKNLVVLMGRIIVVEFFLRYDTFIVDVADLALGPAVKFKSLTRAT 475
           PT ENKQCPGKNLVVL+GRI+VVEFFLRYDTF V+VADL LGPAVKFKSLTRAT
Sbjct: 425 PTPENKQCPGKNLVVLIGRIMVVEFFLRYDTFTVEVADLPLGPAVKFKSLTRAT 478

BLAST of Chy1G000110 vs. NCBI nr
Match: XP_038886895.1 (allene oxide synthase 3-like [Benincasa hispida])

HSP 1 Score: 887 bits (2292), Expect = 0.0
Identity = 440/477 (92.24%), Postives = 455/477 (95.39%), Query Frame = 0

Query: 2   ASSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVFRANMPPGP 61
           +SSSPELPLKPIPGGYGF FLGPIKDRYDYFYFQG+DEFFRSR+TKY STVFRANMPPGP
Sbjct: 5   SSSSPELPLKPIPGGYGFSFLGPIKDRYDYFYFQGKDEFFRSRVTKYGSTVFRANMPPGP 64

Query: 62  FISFDSRVVVLLDALSFPILFDTIKVEKRNILDGTYMPSLSFTGGIRTCAYLDPSETEHS 121
           FIS DSRVVVLLDA+SFPILFDT KVEKRNILDGTYMPSLSFTGGIRTCAYLDPSETEHS
Sbjct: 65  FISSDSRVVVLLDAVSFPILFDTTKVEKRNILDGTYMPSLSFTGGIRTCAYLDPSETEHS 124

Query: 122 VLKRLFLSFLASRHDRFIPLFRSSLSEMFVKLEDKLADKNKIADFNSISDAMSFDYVFRL 181
           VLK LFLSFLASRHDR IPLFRSSLSEMFVKLEDKLA+K KIADFN ISD+MSFDYVFRL
Sbjct: 125 VLKGLFLSFLASRHDRIIPLFRSSLSEMFVKLEDKLAEKKKIADFNPISDSMSFDYVFRL 184

Query: 182 LSDGTPDSKLAADGPGMFDLWLGLQLAPLASIGLPKIFSVFEDLIIHTIPLPFFPVKSGY 241
            SDG PD KLAADGPGMFDLWL  QLAPLASIG+P IFS+FEDLIIHTIPLPFFPVKSGY
Sbjct: 185 FSDGNPDPKLAADGPGMFDLWLAFQLAPLASIGVPNIFSIFEDLIIHTIPLPFFPVKSGY 244

Query: 242 RKLYKAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKILFPTILKWVGTAGE 301
           RKLYKAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMK+LFPTILKWVGTAGE
Sbjct: 245 RKLYKAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPTILKWVGTAGE 304

Query: 302 DIHRKLAEEVRATVKEEGGLTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQS 361
           D+HRKLA+EVR TVKEEGGLTF ALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVI S
Sbjct: 305 DLHRKLADEVRTTVKEEGGLTFGALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIHS 364

Query: 362 HDSSFKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETVE 421
           HDS+FKIKKGETIFGYQPFATKDPKIFKD+EKFVGDRF GEEGEKLLKYVYWSNERETVE
Sbjct: 365 HDSAFKIKKGETIFGYQPFATKDPKIFKDAEKFVGDRFEGEEGEKLLKYVYWSNERETVE 424

Query: 422 PTAENKQCPGKNLVVLMGRIIVVEFFLRYDTFIVDVADLALGPAVKFKSLTRATAAV 478
           PTAENKQCPGKNLVVLMGRII+VEFFLRYDTF VDVADL LGPAVKFKSLT+AT  V
Sbjct: 425 PTAENKQCPGKNLVVLMGRIILVEFFLRYDTFTVDVADLPLGPAVKFKSLTKATVKV 481

BLAST of Chy1G000110 vs. TAIR 10
Match: AT5G42650.1 (allene oxide synthase )

HSP 1 Score: 512.3 bits (1318), Expect = 4.2e-145
Identity = 257/475 (54.11%), Postives = 335/475 (70.53%), Query Frame = 0

Query: 3   SSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVFRANMPPGPF 62
           + S +LP++ IPG YG P +GPIKDR+DYFY QG +EFF+SRI KYNSTV+R NMPPG F
Sbjct: 47  TGSKDLPIRNIPGNYGLPIVGPIKDRWDYFYDQGAEEFFKSRIRKYNSTVYRVNMPPGAF 106

Query: 63  ISFDSRVVVLLDALSFPILFDTIKVEKRNILDGTYMPSLSFTGGIRTCAYLDPSETEHSV 122
           I+ + +VV LLD  SFP+LFD  KVEK+++  GTYMPS   TGG R  +YLDPSE +H  
Sbjct: 107 IAENPQVVALLDGKSFPVLFDVDKVEKKDLFTGTYMPSTELTGGYRILSYLDPSEPKHEK 166

Query: 123 LKRLFLSFLASRHDRFIPLFRSSLSEMFVKLEDKLADKNKIADFNSISDAMSFDYVFRLL 182
           LK L    L S  +R  P F+++ SE+F  LE +L+ K K ADF   SD  +F+++ R  
Sbjct: 167 LKNLLFFLLKSSRNRIFPEFQATYSELFDSLEKELSLKGK-ADFGGSSDGTAFNFLARAF 226

Query: 183 SDGTP-DSKLAADGPGMFDLWLGLQLAPLASIGLPKIFSVFEDLIIHTIPLPFFPVKSGY 242
               P D+KL AD PG+   W+   L PL SIGLP+   V E+ +IHT  LP   VKS Y
Sbjct: 227 YGTNPADTKLKADAPGLITKWVLFNLHPLLSIGLPR---VIEEPLIHTFSLPPALVKSDY 286

Query: 243 RKLYKAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKILFPTILKWVGTAGE 302
           ++LY+ F  S+G  L EA+K GI RE+A HNL+F   FN +GGMKILFP ++K +G AG 
Sbjct: 287 QRLYEFFLESAGEILVEADKLGISREEATHNLLFATCFNTWGGMKILFPNMVKRIGRAGH 346

Query: 303 DIHRKLAEEVRATVKEEGG-LTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQ 362
            +H +LAEE+R+ +K  GG LT  A+EKM L KSVVYE LR EPPV  QYG+AK+D+VI+
Sbjct: 347 QVHNRLAEEIRSVIKSNGGELTMGAIEKMELTKSVVYECLRFEPPVTAQYGRAKKDLVIE 406

Query: 363 SHDSSFKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETV 422
           SHD++FK+K GE ++GYQP AT+DPKIF  +++FV +RFVGEEGEKLL++V WSN  ET 
Sbjct: 407 SHDAAFKVKAGEMLYGYQPLATRDPKIFDRADEFVPERFVGEEGEKLLRHVLWSNGPETE 466

Query: 423 EPTAENKQCPGKNLVVLMGRIIVVEFFLRYDTFIVDVADLALGPAVKFKSLTRAT 476
            PT  NKQC GK+ VVL+ R+ V+E F RYD+F ++V    LG +V F SL +A+
Sbjct: 467 TPTVGNKQCAGKDFVVLVARLFVIEIFRRYDSFDIEVGTSPLGSSVNFSSLRKAS 517

BLAST of Chy1G000110 vs. TAIR 10
Match: AT4G15440.1 (hydroperoxide lyase 1 )

HSP 1 Score: 257.7 bits (657), Expect = 1.9e-68
Identity = 133/373 (35.66%), Postives = 213/373 (57.10%), Query Frame = 0

Query: 87  VEKRNILDGTYMPSLSFTGGIRTCAYLDPSETEHSVLKRLFLSFLASRHDRFIPLFRSSL 146
           V+KR++L G + PSL F GG+R   YLD +E +H+ +K   +  L      ++   RS+L
Sbjct: 4   VDKRDVLIGDFRPSLGFYGGVRVGVYLDTTEPKHAKIKGFAMETLKRSSKVWLQELRSNL 63

Query: 147 SEMFVKLEDKLADKNKIADFNSISDAMSFDYVFRLLS--DGTPDSKLAADGPGMFDLWLG 206
           +  +  +E +++ KN  A +        F ++   L+  D +    +A +G    + WL 
Sbjct: 64  NIFWGTIESEIS-KNGAASYIFPLQRCIFSFLCASLAGVDASVSPDIAENGWKTINTWLA 123

Query: 207 LQLAPLASIGLPKIFSVFEDLIIHTIPLPFFPVKSGYRKLYKAFYSSSGSFLDEAEKQ-G 266
           LQ+ P A +G+  +    E++++HT P P   +   Y+KLY     ++G  L   +++ G
Sbjct: 124 LQVIPTAKLGV--VPQPLEEILLHTWPYPSLLIAGNYKKLYNFIDENAGDCLRLGQEEFG 183

Query: 267 IDREKACHNLVFLAGFNAYGGMKILFPTILKWVGTAGEDIHRKLAEEVRATVKEEGGLTF 326
           + R++A  NL+F+ GFNAYGG  +  P+++  +      +  ++  EVR        L F
Sbjct: 184 LTRDEAIQNLLFVLGFNAYGGFSVFLPSLIGRITGDNSGLQERIRTEVRRVCGSGSDLNF 243

Query: 327 SALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQSHDSSFKIKKGETIFGYQPFATK 386
             + +M L+KSVVYE LR  PPVP Q+ +A++D  I SHD+ F++KKGE + GYQP   +
Sbjct: 244 KTVNEMELVKSVVYETLRFSPPVPLQFARARKDFQISSHDAVFEVKKGELLCGYQPLVMR 303

Query: 387 DPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETVEPTAENKQCPGKNLVVLMGRIIV 446
           D  +F + E+F  DR+VGE G +LL Y+YWSN  +T  P+A NKQC  K++V L   ++V
Sbjct: 304 DANVFDEPEEFKPDRYVGETGSELLNYLYWSNGPQTGTPSASNKQCAAKDIVTLTASLLV 363

Query: 447 VEFFLRYDTFIVD 457
            + FLRYDT   D
Sbjct: 364 ADLFLRYDTITGD 373

BLAST of Chy1G000110 vs. TAIR 10
Match: AT2G30750.1 (cytochrome P450, family 71, subfamily A, polypeptide 12 )

HSP 1 Score: 52.4 bits (124), Expect = 1.2e-06
Identity = 40/167 (23.95%), Postives = 78/167 (46.71%), Query Frame = 0

Query: 291 TILKWVGT---AGEDIHRKLAEEVRATVKEEGG-LTFSALEKMSLLKSVVYEALRIEPPV 350
           T+L+W+ T      ++ +KL +E+R+T++  G  +    +E M  LK+V+ E  R+ PP+
Sbjct: 311 TLLEWIMTELIRNPNVMKKLQDEIRSTIRPHGSYIKEKDVENMKYLKAVIKEVFRVHPPL 370

Query: 351 PFQYGK-AKEDIVIQSHDSSFKIKKGETIFGYQPFATKDPKIF-KDSEKFVGDRFVGEEG 410
           P    +   ED+ ++     + I  G  +        +DP I+  D+E+F  +R +    
Sbjct: 371 PLILPRLLSEDVKVK----GYNIAAGTEVIINAWAIQRDPAIWGPDAEEFKPERHLDSTL 430

Query: 411 EKLLKYVYWSNERETVEPTAENKQCPGKNLVVLMGRIIVVEFFLRYD 452
           +      Y   +   +   +  + CPG NL + +  + V     R+D
Sbjct: 431 D------YHGKDLNFIPFGSGRRICPGINLALGLVEVTVANLVGRFD 467

BLAST of Chy1G000110 vs. TAIR 10
Match: AT1G11610.1 (cytochrome P450, family 71, subfamily A, polypeptide 18 )

HSP 1 Score: 51.6 bits (122), Expect = 2.0e-06
Identity = 43/170 (25.29%), Postives = 81/170 (47.65%), Query Frame = 0

Query: 291 TILKWVGT---AGEDIHRKLAEEVRATVKEEGG-LTFSALEKMSLLKSVVYEALRIEPPV 350
           T+L+W+ T      +  +KL  E+R+T++  G  +    +E M  LK+V+ E  R+ PP+
Sbjct: 305 TLLEWIMTELIRNPECMKKLQNEIRSTIRPHGSYIKEKEVENMRYLKAVIKEVFRVHPPL 364

Query: 351 PFQYGK-AKEDIVIQSHDSSFKIKKGETIFGYQPFATKDPKIF-KDSEKFVGDRFVGEEG 410
           P    +   ED+ ++ +D    I  G  +        +DP I+  D+E+F  +R +    
Sbjct: 365 PLILPRLLTEDVKVKGYD----IAAGTEVLINAWSIHRDPAIWGPDAEEFKPERHLDSTL 424

Query: 411 E---KLLKYVYWSNERETVEPTAENKQCPGKNLVVLMGRIIVVEFFLRYD 452
           +   + LKY+ + + R         + CPG NL + +  + +     R+D
Sbjct: 425 DYHGQDLKYIPFGSGR---------RICPGINLAMGLVEVTLANLVGRFD 461

BLAST of Chy1G000110 vs. TAIR 10
Match: AT1G11610.2 (cytochrome P450, family 71, subfamily A, polypeptide 18 )

HSP 1 Score: 51.6 bits (122), Expect = 2.0e-06
Identity = 43/170 (25.29%), Postives = 81/170 (47.65%), Query Frame = 0

Query: 291 TILKWVGT---AGEDIHRKLAEEVRATVKEEGG-LTFSALEKMSLLKSVVYEALRIEPPV 350
           T+L+W+ T      +  +KL  E+R+T++  G  +    +E M  LK+V+ E  R+ PP+
Sbjct: 305 TLLEWIMTELIRNPECMKKLQNEIRSTIRPHGSYIKEKEVENMRYLKAVIKEVFRVHPPL 364

Query: 351 PFQYGK-AKEDIVIQSHDSSFKIKKGETIFGYQPFATKDPKIF-KDSEKFVGDRFVGEEG 410
           P    +   ED+ ++ +D    I  G  +        +DP I+  D+E+F  +R +    
Sbjct: 365 PLILPRLLTEDVKVKGYD----IAAGTEVLINAWSIHRDPAIWGPDAEEFKPERHLDSTL 424

Query: 411 E---KLLKYVYWSNERETVEPTAENKQCPGKNLVVLMGRIIVVEFFLRYD 452
           +   + LKY+ + + R         + CPG NL + +  + +     R+D
Sbjct: 425 DYHGQDLKYIPFGSGR---------RICPGINLAMGLVEVTLANLVGRFD 461

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8GZP51.3e-15956.93Allene oxide synthase 3 OS=Solanum lycopersicum OX=4081 GN=AOS3 PE=1 SV=1[more]
K4BV523.0e-14855.09Allene oxide synthase 1, chloroplastic OS=Solanum lycopersicum OX=4081 GN=AOS1 P... [more]
Q962425.9e-14454.11Allene oxide synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CYP74A P... [more]
Q7XYS31.5e-14252.95Allene oxide synthase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP74A2 PE=2... [more]
Q9LLB02.1e-14152.98Allene oxide synthase 2, chloroplastic OS=Solanum lycopersicum OX=4081 GN=AOS2 P... [more]
Match NameE-valueIdentityDescription
U5YTM62.0e-26796.86Hydroperoxide lyase OS=Cucumis sativus OX=3659 GN=HPL PE=2 SV=1[more]
Q9M5J22.2e-26696.44Fatty acid hydroperoxide lyase OS=Cucumis sativus OX=3659 PE=2 SV=1[more]
A0A5D3C5U21.1e-26294.97Allene oxide synthase 2-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A1S3BZK91.1e-26294.97allene oxide synthase 2-like isoform X1 OS=Cucumis melo OX=3656 GN=HPL PE=4 SV=1[more]
Q93XR34.2e-26294.76Fatty acid 9-hydroperoxide lyase OS=Cucumis melo OX=3656 GN=HPL PE=2 SV=1[more]
Match NameE-valueIdentityDescription
NP_001274399.10.096.86allene oxide synthase, chloroplastic-like [Cucumis sativus] >XP_011658536.1 alle... [more]
AAF64041.10.096.44fatty acid hydroperoxide lyase [Cucumis sativus][more]
XP_008454885.10.095.36PREDICTED: allene oxide synthase 2-like isoform X1 [Cucumis melo] >KAA0031259.1 ... [more]
NP_001284390.10.095.15allene oxide synthase 2-like [Cucumis melo] >AAK54282.1 fatty acid 9-hydroperoxi... [more]
XP_038886895.10.092.24allene oxide synthase 3-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT5G42650.14.2e-14554.11allene oxide synthase [more]
AT4G15440.11.9e-6835.66hydroperoxide lyase 1 [more]
AT2G30750.11.2e-0623.95cytochrome P450, family 71, subfamily A, polypeptide 12 [more]
AT1G11610.12.0e-0625.29cytochrome P450, family 71, subfamily A, polypeptide 18 [more]
AT1G11610.22.0e-0625.29cytochrome P450, family 71, subfamily A, polypeptide 18 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002403Cytochrome P450, E-class, group IVPRINTSPR00465EP450IVcoord: 329..345
score: 37.78
coord: 382..400
score: 31.58
coord: 429..447
score: 21.26
IPR001128Cytochrome P450PFAMPF00067p450coord: 302..412
e-value: 2.7E-16
score: 59.4
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 8..477
e-value: 7.8E-109
score: 366.7
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 9..456
NoneNo IPR availablePANTHERPTHR24286CYTOCHROME P450 26coord: 7..455
NoneNo IPR availablePANTHERPTHR24286:SF302HYDROPEROXIDE LYASEcoord: 7..455

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy1G000110.1Chy1G000110.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016125 sterol metabolic process
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016829 lyase activity
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen