Chy1G000040 (gene) Cucumber (hystrix) v1

Overview
NameChy1G000040
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
Description4-hydroxy-3-methylbut-2-enyl diphosphate reductase
LocationchrH01: 32580 .. 36497 (-)
RNA-Seq ExpressionChy1G000040
SyntenyChy1G000040
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGTTCTCTATGCAACTTTATCGTCTTCCCACTCGTACGGATGCAGCCATGCCTGAGAATTTCACCGCCGTTCGATCACTCCGCTTCAGGAAGCCTATGACTGTGAGATGCGATGGTGATTCTTCTTCTTCTTCTTCCGCGGCTGTGGATTCCAATTTTGACGCCAAGGTGTTCCGGAGGAACTTGACGAAGAGTGACAACTACAACCGCAAAGGCTTTGGTTACAAGGACGAAACTCTGGCGCTTATGAATCGCGAGTATACAAGTAAGCCTCGATCGTCTTGAATCTTTTAGATTTCTGATTGAATTAGGAAAATGATTTATGAATTCTTTGTTTTCATTATTTTGCATGTCAGAGAATATTTCTATCTCCTGAAGAATTTTAGTCTTTCGACTTGGCTTCTCAAAGCCTTAAAAAAAAAAAAGAAAAAAAAAAAAAAAAAAGAAGAAGAAGAATAAGAAGAAGAAGGGGGTAGAGGCTTATCATTTGAATTTTCAACTTATTTTTATGAAAATAAGTTTTTTTTAAGGAAGCTTGTGTTTTCTAATTCTATGTTCGAATTTCAGGTGATATCATAAAGACTCTCAAAGAAAATGGATTTGAGTATACGTGGGGAGATGTTACTGTAAAACTGGCGGAAGCGTATGGATTTTGTTGGGGAGTCGAGCGAGCAGTTCAGATTGCTTATGAAGCGAGAAAACAATTTCCTGATGAGAAAATCTGGTTAACTAATGAGATTATTCACAATCCAACTGTTAATAAGGTCTTGATTCTTTAGATTTTTTAAATCTAGAAATGAATCTTACTCTGCTAAACTTTATGATGCTTATTGTGTTCTATTTGGTAGAGGGGAAAGAAAGTATTCTTTGTGTAATATTTTATTGTCCAATGACGAGTTGTTATGATCTGTTCTACTTTCAATCAGAGGTGTTTCTTTTATCCCTTTCTGGGATCTTCCCCTTTCTTTCACTGAACCTGTTAGGTATCAAGTTCCCTCAACACAGAAAATACCCAACAAGGCCACTCACAGAGGCAATAAACTCTGTTTATGATACTCAGAGAAAGGAAGCAGCCAACAATTACAAGATAGTTCAAATATCCAAAAAGAAACAAGAACTCAGTGACAAGTAGAAGAACAAGGAAGAAATAAAACACAAGAAAAAGATATGACAGTATTCGAGAGCTGCCTCTTCTCCCAAAAACTTCAAAAGCTTTCCAAATTCATCACCCAAAATGATTCCCCAAACCCCTTCATAAACCTCTTGCCGATGGTTCCCACCTCATGTCACAATTCTTTCTGTATCAACATTCTCTTTAATTCCCAGCTTTCCTCTTTTACCCCTCCTCTGGTATGTGTGAATAATGGGAGGTCTAACAGAATCATGCATCTTATTTGTGGAGGAAGTTCAAGTTTTCAATTACTGGTTTATTACTGACTAGTGTGCTTTGACATCTCTCTTTTATTCATCTAGTGATAACATACTCCAGCCAGAAAAAGGCGATCTGGTTTTTATTTTGAATTTTCATTTCCATTCCCAACTATGCATTTTTTATCAGTCTGAACTGTGATTTGAAAATTTGGCAAGATGGTATCTCAGTTGCTGCCCTGGTCCCATGAGTGTGGTTTTCAGTATGGTGAATTGACTATGAGGATTGAAGAGCTTTGACCTTCCAGTCTTTGGGAACAATGCAATTCTCACCCCTAATTTTTTGTGGAATTCTAAATTTTATCTTTTTGATCTTTTTATTTTCTAAATTCTAATCATGTTTCCATCTAGAATATGGAGTAGGATAGACTTGTTGACCAACTTGTTGTGTTTGGAATCAGTAAAAGTCTTGCACCAATACTTGGATTTTTGTTAGATCTTTGAAAATTTATCTTTACTGCCGTCCTTTTCCTAGAAACTTTGAAATATTCCTTCTTTGGATTTCCTTGCTGTCTTTTCCAACAAAAGAACATCTTGGTGTATGACTGGTGTTCAAAAGTTTCTAAACTTGATTGTTTGTTACCATCCTTTCGCATAACTCTTAGTTTTCTTGTTACACGAATGTAGAAATTTAATAGATATTTTCGAATTCTACATGTAGCGTTTGGAGGAGATGGAAGTCCAAAACATTCCACTGGAGGAAGGAAGGAAACAGTTTGAAGTGGTTGATAAGGGTGATGTGGTGATTTTGCCTGCATTTGGAGCCGCGGTGGATGAGATGTTGACATTGAGTGAAAAAAAGGTGCAAATCGTTGATACAACTTGCCCATGGGTTTCAAAGGTAATGTAGCCGTTTTTTCCCCCTCCTTGCTGTACGTTCTGATACTGACAATAATGCTGCACTGCAATCAATATAAAGTTTGTTGCATCTGACAAAATTACTCTCTCGATCCATACATCGGCTGCACAACATTTGAAGGTTTGGAATGTTGTTGACAAGCACAAAAAAGGTGATTATACTTCAATCATTCACGGCAAATATGCTCATGAAGAAACTGTAGCGACAGCATCATTTGCTGGGACATATATAATTGTAAAGAACATGGCAGAGGTATGTCCATTTCGTTTTTCTTTTTTATTGACCACAGCAATTCTGCAATTTGCTGGGCGAGGAATAATGTGAATTTGTTGGGTAAACTTTTGCCTTTATTTATTCAGGCAATATATGTGTGTGACTATATTCTTGGAGGTGAACTTGATGGATCCAGTTCAACCAAAGGAGAGTTTATGAAGGTATTATTTACAATACTTTCTTAATCATTATATATTGGGATCATTCACATTTCAGAGCTTCATTGGTAACAATTCCTGGTCTGCAGAAATTCAAAAATGCAGTCTCAAAAGGTTTTGACCCAGATGTTGACCTGTTAAAGGTTGGGATAGCAAACCAAACCACAATGCTCAAGGGAGAAACAGAAGAGATTGGTAAATTATCTGCTGAAACACCATTCATTGCTGTTCCTAGAAATGTCAAGCATATTTATTTAAATTTTACTTTTATCAGGTAAATTAGTTGAAAGAACTATGATGCGCAAGCATGGAGTTGAAAACGTCAATGAGCACTTCCTCAGTTTCAATACAATATGTGATGCTACTCAAGTAAGTCTAGTCGGATTTGCTGCGGTGTCTAGGATGTGGATTTTAGGGAGTAAAATGTGTTTAGTATTCTATGTTTTACTGCTTCAGTGCACCTGCGCTCACTTGTGGTATTATCAACTAAACTCTTATCCTTTGTTACCATCTTTGTCCAGTCCTGTCACGATTCTATTGACAAGTTCGCTAACCCGTGTATGTTTATCTCGTATACATTTAGTTGTCACTTATGTGTTCTCTAACCTGTCTTATTCTATCAGGAGCGTCAAGATGCAATGTACAAGCTAGTAGAGGGAAAACTGGATCTCATGTTAGTTGTTGGTGGATGGAATTCCAGTAACACTTCTCATCTTCAAGAGATAGCGGAGGAGCGAGGAATTCCATCTTACTGGATTGATAGTGAGCAGAGAATAGGTCCAGGAAACAGAATAGCTTACAAACTAAATGTAAGTTAACATACAGATTTCAGTTCTAATTCTCTTTTAGACACAATTTCCAAACTCTTTGTTCACTTTTCAATTACAAAATAAACCTAAGTTTTGTTTCCTTGATTACTTACAATTTCACCATCCTTGCAAATTCATGTTTAGCATGGGGAGTTAGTTGAAAAGGAGAACTGGCTACCCAAGGGTCATATCACGATTGGTGTCACATCCGGGGCTTCAACCCCCGATAAGGTTAGTCTCTCCGACTTATATGCTTGTTAGTACAAACTGTTATCTGCAACAAATTTAAAGCCATACCATGTTTGTATGTGCTTTCAAACAGGTCGTAGAAGATGCTCTTATCAAAGTCTTCGACATTAAGCGCGAGGAAGCTTTGCAGTTTGCATAA

mRNA sequence

ATGGCGTTCTCTATGCAACTTTATCGTCTTCCCACTCGTACGGATGCAGCCATGCCTGAGAATTTCACCGCCGTTCGATCACTCCGCTTCAGGAAGCCTATGACTGTGAGATGCGATGGTGATTCTTCTTCTTCTTCTTCCGCGGCTGTGGATTCCAATTTTGACGCCAAGGTGTTCCGGAGGAACTTGACGAAGAGTGACAACTACAACCGCAAAGGCTTTGGTTACAAGGACGAAACTCTGGCGCTTATGAATCGCGAGTATACAAGTGATATCATAAAGACTCTCAAAGAAAATGGATTTGAGTATACGTGGGGAGATGTTACTGTAAAACTGGCGGAAGCGTATGGATTTTGTTGGGGAGTCGAGCGAGCAGTTCAGATTGCTTATGAAGCGAGAAAACAATTTCCTGATGAGAAAATCTGGTTAACTAATGAGATTATTCACAATCCAACTGTTAATAAGCGTTTGGAGGAGATGGAAGTCCAAAACATTCCACTGGAGGAAGGAAGGAAACAGTTTGAAGTGGTTGATAAGGGTGATGTGGTGATTTTGCCTGCATTTGGAGCCGCGGTGGATGAGATGTTGACATTGAGTGAAAAAAAGGTGCAAATCGTTGATACAACTTGCCCATGGGTTTCAAAGGTTTGGAATGTTGTTGACAAGCACAAAAAAGGTGATTATACTTCAATCATTCACGGCAAATATGCTCATGAAGAAACTGTAGCGACAGCATCATTTGCTGGGACATATATAATTGTAAAGAACATGGCAGAGGCAATATATGTGTGTGACTATATTCTTGGAGGTGAACTTGATGGATCCAGTTCAACCAAAGGAGAGTTTATGAAGAAATTCAAAAATGCAGTCTCAAAAGGTTTTGACCCAGATGTTGACCTGTTAAAGGTTGGGATAGCAAACCAAACCACAATGCTCAAGGGAGAAACAGAAGAGATTGGTAAATTAGTTGAAAGAACTATGATGCGCAAGCATGGAGTTGAAAACGTCAATGAGCACTTCCTCAGTTTCAATACAATATGTGATGCTACTCAAGAGCGTCAAGATGCAATGTACAAGCTAGTAGAGGGAAAACTGGATCTCATGTTAGTTGTTGGTGGATGGAATTCCAGTAACACTTCTCATCTTCAAGAGATAGCGGAGGAGCGAGGAATTCCATCTTACTGGATTGATAGTGAGCAGAGAATAGGTCCAGGAAACAGAATAGCTTACAAACTAAATCATGGGGAGTTAGTTGAAAAGGAGAACTGGCTACCCAAGGGTCATATCACGATTGGTGTCACATCCGGGGCTTCAACCCCCGATAAGGTCGTAGAAGATGCTCTTATCAAAGTCTTCGACATTAAGCGCGAGGAAGCTTTGCAGTTTGCATAA

Coding sequence (CDS)

ATGGCGTTCTCTATGCAACTTTATCGTCTTCCCACTCGTACGGATGCAGCCATGCCTGAGAATTTCACCGCCGTTCGATCACTCCGCTTCAGGAAGCCTATGACTGTGAGATGCGATGGTGATTCTTCTTCTTCTTCTTCCGCGGCTGTGGATTCCAATTTTGACGCCAAGGTGTTCCGGAGGAACTTGACGAAGAGTGACAACTACAACCGCAAAGGCTTTGGTTACAAGGACGAAACTCTGGCGCTTATGAATCGCGAGTATACAAGTGATATCATAAAGACTCTCAAAGAAAATGGATTTGAGTATACGTGGGGAGATGTTACTGTAAAACTGGCGGAAGCGTATGGATTTTGTTGGGGAGTCGAGCGAGCAGTTCAGATTGCTTATGAAGCGAGAAAACAATTTCCTGATGAGAAAATCTGGTTAACTAATGAGATTATTCACAATCCAACTGTTAATAAGCGTTTGGAGGAGATGGAAGTCCAAAACATTCCACTGGAGGAAGGAAGGAAACAGTTTGAAGTGGTTGATAAGGGTGATGTGGTGATTTTGCCTGCATTTGGAGCCGCGGTGGATGAGATGTTGACATTGAGTGAAAAAAAGGTGCAAATCGTTGATACAACTTGCCCATGGGTTTCAAAGGTTTGGAATGTTGTTGACAAGCACAAAAAAGGTGATTATACTTCAATCATTCACGGCAAATATGCTCATGAAGAAACTGTAGCGACAGCATCATTTGCTGGGACATATATAATTGTAAAGAACATGGCAGAGGCAATATATGTGTGTGACTATATTCTTGGAGGTGAACTTGATGGATCCAGTTCAACCAAAGGAGAGTTTATGAAGAAATTCAAAAATGCAGTCTCAAAAGGTTTTGACCCAGATGTTGACCTGTTAAAGGTTGGGATAGCAAACCAAACCACAATGCTCAAGGGAGAAACAGAAGAGATTGGTAAATTAGTTGAAAGAACTATGATGCGCAAGCATGGAGTTGAAAACGTCAATGAGCACTTCCTCAGTTTCAATACAATATGTGATGCTACTCAAGAGCGTCAAGATGCAATGTACAAGCTAGTAGAGGGAAAACTGGATCTCATGTTAGTTGTTGGTGGATGGAATTCCAGTAACACTTCTCATCTTCAAGAGATAGCGGAGGAGCGAGGAATTCCATCTTACTGGATTGATAGTGAGCAGAGAATAGGTCCAGGAAACAGAATAGCTTACAAACTAAATCATGGGGAGTTAGTTGAAAAGGAGAACTGGCTACCCAAGGGTCATATCACGATTGGTGTCACATCCGGGGCTTCAACCCCCGATAAGGTCGTAGAAGATGCTCTTATCAAAGTCTTCGACATTAAGCGCGAGGAAGCTTTGCAGTTTGCATAA

Protein sequence

MAFSMQLYRLPTRTDAAMPENFTAVRSLRFRKPMTVRCDGDSSSSSSAAVDSNFDAKVFRRNLTKSDNYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYTWGDVTVKLAEAYGFCWGVERAVQIAYEARKQFPDEKIWLTNEIIHNPTVNKRLEEMEVQNIPLEEGRKQFEVVDKGDVVILPAFGAAVDEMLTLSEKKVQIVDTTCPWVSKVWNVVDKHKKGDYTSIIHGKYAHEETVATASFAGTYIIVKNMAEAIYVCDYILGGELDGSSSTKGEFMKKFKNAVSKGFDPDVDLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDATQERQDAMYKLVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHGELVEKENWLPKGHITIGVTSGASTPDKVVEDALIKVFDIKREEALQFA*
Homology
BLAST of Chy1G000040 vs. ExPASy Swiss-Prot
Match: Q6AVG6 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISPH PE=2 SV=1)

HSP 1 Score: 740.3 bits (1910), Expect = 1.3e-212
Identity = 358/435 (82.30%), Postives = 404/435 (92.87%), Query Frame = 0

Query: 31  RKPMTVRCDGD--SSSSSSAAVDSNFDAKVFRRNLTKSDNYNRKGFGYKDETLALMNREY 90
           R P +VRCD    SS S+SA++D++FD K FR NLT+SDNYNRKGFG+K ETL LM++EY
Sbjct: 25  RAPSSVRCDSSAASSLSASASLDADFDKKQFRHNLTRSDNYNRKGFGHKKETLELMSQEY 84

Query: 91  TSDIIKTLKENGFEYTWGDVTVKLAEAYGFCWGVERAVQIAYEARKQFPDEKIWLTNEII 150
           TSD+IKTLKENG ++TWG VTVKLAEAYGFCWGVERAVQIAYEARKQFPD++IWLTNEII
Sbjct: 85  TSDVIKTLKENGNQHTWGPVTVKLAEAYGFCWGVERAVQIAYEARKQFPDDRIWLTNEII 144

Query: 151 HNPTVNKRLEEMEVQNIPLEEGRKQFEVVDKGDVVILPAFGAAVDEMLTLSEKKVQIVDT 210
           HNPTVNKRLE+M VQNIP++ G K F+VV++GDVV+LPAFGAAV+EM TL+EKKVQIVDT
Sbjct: 145 HNPTVNKRLEDMGVQNIPVDAGIKDFDVVEQGDVVVLPAFGAAVEEMYTLNEKKVQIVDT 204

Query: 211 TCPWVSKVWNVVDKHKKGDYTSIIHGKYAHEETVATASFAGTYIIVKNMAEAIYVCDYIL 270
           TCPWVSKVWN+V+KHKKGDYTSIIHGKY+HEETVATASFAGTYIIVKN+AEA YVCDYIL
Sbjct: 205 TCPWVSKVWNMVEKHKKGDYTSIIHGKYSHEETVATASFAGTYIIVKNIAEASYVCDYIL 264

Query: 271 GGELDGSSSTKGEFMKKFKNAVSKGFDPDVDLLKVGIANQTTMLKGETEEIGKLVERTMM 330
           GG+LDGSSSTK EF++KFKNAVS GFDPDVDL+KVGIANQTTMLKGETEEIGKLVE+TMM
Sbjct: 265 GGQLDGSSSTKEEFLEKFKNAVSPGFDPDVDLVKVGIANQTTMLKGETEEIGKLVEKTMM 324

Query: 331 RKHGVENVNEHFLSFNTICDATQERQDAMYKLVEGKLDLMLVVGGWNSSNTSHLQEIAEE 390
           R+ GVENVN+HF++FNTICDATQERQDAMY+LV+ K+DL+LVVGGWNSSNTSHLQEI E 
Sbjct: 325 RRFGVENVNDHFIAFNTICDATQERQDAMYQLVKEKVDLILVVGGWNSSNTSHLQEIGEL 384

Query: 391 RGIPSYWIDSEQRIGPGNRIAYKLNHGELVEKENWLPKGHITIGVTSGASTPDKVVEDAL 450
            GIPSYWIDSEQRIGPGN+I+YKLNHGELVEKENWLP+G ITIGVTSGASTPDKVVEDAL
Sbjct: 385 SGIPSYWIDSEQRIGPGNKISYKLNHGELVEKENWLPEGPITIGVTSGASTPDKVVEDAL 444

Query: 451 IKVFDIKREEALQFA 464
            KVF+IKR+E LQ A
Sbjct: 445 QKVFEIKRQEVLQAA 459

BLAST of Chy1G000040 vs. ExPASy Swiss-Prot
Match: Q94B35 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISPH PE=1 SV=1)

HSP 1 Score: 738.4 bits (1905), Expect = 4.9e-212
Identity = 359/466 (77.04%), Postives = 417/466 (89.48%), Query Frame = 0

Query: 1   MAFSMQLYRLPTRTDAAMPEN-FTAVRSLRFRKPMTVRCDG--DSSSSSSAAVDSNFDAK 60
           MA ++Q  RL  R D  + EN  +   SLR RK ++VRC    +++ S S  +DS+FDAK
Sbjct: 1   MAVALQFSRLCVRPDTFVRENHLSGSGSLRRRKALSVRCSSGDENAPSPSVVMDSDFDAK 60

Query: 61  VFRRNLTKSDNYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYTWGDVTVKLAEAYG 120
           VFR+NLT+SDNYNRKGFG+K+ETL LMNREYTSDI++TLK NG+ Y+WGDVTVKLA+AYG
Sbjct: 61  VFRKNLTRSDNYNRKGFGHKEETLKLMNREYTSDILETLKTNGYTYSWGDVTVKLAKAYG 120

Query: 121 FCWGVERAVQIAYEARKQFPDEKIWLTNEIIHNPTVNKRLEEMEVQNIPLEEGRKQFEVV 180
           FCWGVERAVQIAYEARKQFP+E++W+TNEIIHNPTVNKRLE+M+V+ IP+E+ +KQF+VV
Sbjct: 121 FCWGVERAVQIAYEARKQFPEERLWITNEIIHNPTVNKRLEDMDVKIIPVEDSKKQFDVV 180

Query: 181 DKGDVVILPAFGAAVDEMLTLSEKKVQIVDTTCPWVSKVWNVVDKHKKGDYTSIIHGKYA 240
           +K DVVILPAFGA VDEM  L++KKVQIVDTTCPWV+KVWN V+KHKKG+YTS+IHGKY 
Sbjct: 181 EKDDVVILPAFGAGVDEMYVLNDKKVQIVDTTCPWVTKVWNTVEKHKKGEYTSVIHGKYN 240

Query: 241 HEETVATASFAGTYIIVKNMAEAIYVCDYILGGELDGSSSTKGEFMKKFKNAVSKGFDPD 300
           HEET+ATASFAG YIIVKNM EA YVCDYILGG+ DGSSSTK EFM+KFK A+SKGFDPD
Sbjct: 241 HEETIATASFAGKYIIVKNMKEANYVCDYILGGQYDGSSSTKEEFMEKFKYAISKGFDPD 300

Query: 301 VDLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDATQERQDAM 360
            DL+KVGIANQTTMLKGETEEIG+L+E TMMRK+GVENV+ HF+SFNTICDATQERQDA+
Sbjct: 301 NDLVKVGIANQTTMLKGETEEIGRLLETTMMRKYGVENVSGHFISFNTICDATQERQDAI 360

Query: 361 YKLVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHGEL 420
           Y+LVE K+DLMLVVGGWNSSNTSHLQEI+E RGIPSYWIDSE+RIGPGN+IAYKL++GEL
Sbjct: 361 YELVEEKIDLMLVVGGWNSSNTSHLQEISEARGIPSYWIDSEKRIGPGNKIAYKLHYGEL 420

Query: 421 VEKENWLPKGHITIGVTSGASTPDKVVEDALIKVFDIKREEALQFA 464
           VEKEN+LPKG ITIGVTSGASTPDKVVEDAL+KVFDIKREE LQ A
Sbjct: 421 VEKENFLPKGPITIGVTSGASTPDKVVEDALVKVFDIKREELLQLA 466

BLAST of Chy1G000040 vs. ExPASy Swiss-Prot
Match: B1WTZ2 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Crocosphaera subtropica (strain ATCC 51142 / BH68) OX=43989 GN=ispH PE=3 SV=1)

HSP 1 Score: 535.4 bits (1378), Expect = 6.3e-151
Identity = 258/406 (63.55%), Postives = 322/406 (79.31%), Query Frame = 0

Query: 55  DAKVFRRNLTKSDNYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYTWGDVTVKLAE 114
           D K F+R+L +S+NY+RKGFG++ E +  MN EY S +I+ ++EN +++  GDVT+KLAE
Sbjct: 2   DTKAFKRSLQQSNNYHRKGFGHETEVMGTMNTEYQSSLIQKIRENNYQWQEGDVTIKLAE 61

Query: 115 AYGFCWGVERAVQIAYEARKQFPDEKIWLTNEIIHNPTVNKRLEEMEVQNIPLEEGRKQF 174
           A+GFCWGVERAV +AYE R+ FP EKIW+TNEIIHNP+VN+RL EM V  I +  G K F
Sbjct: 62  AFGFCWGVERAVAMAYETRQHFPQEKIWITNEIIHNPSVNQRLLEMNVGFIEVINGSKDF 121

Query: 175 EVVDKGDVVILPAFGAAVDEMLTLSEKKVQIVDTTCPWVSKVWNVVDKHKKGDYTSIIHG 234
            VV+ GDVVILPAFGA+V EM  L++K   IVDTTCPWVSKVWN V+KHKK DYTSIIHG
Sbjct: 122 SVVEAGDVVILPAFGASVTEMQLLNDKGCTIVDTTCPWVSKVWNSVEKHKKKDYTSIIHG 181

Query: 235 KYAHEETVATASFAGTYIIVKNMAEAIYVCDYILGGELDGSSSTKGEFMKKFKNAVSKGF 294
           KY HEETVAT+SFAGTY++V N+ EA YVCDYIL G        K EF++KFKNA S+GF
Sbjct: 182 KYKHEETVATSSFAGTYLVVLNLKEAQYVCDYILNG------GNKDEFLEKFKNAHSEGF 241

Query: 295 DPDVDLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDATQERQ 354
           DPD DL+++GIANQTTMLK ETE+IGKL E TM++K+G   +NEHF+SFNTICDATQERQ
Sbjct: 242 DPDKDLIRLGIANQTTMLKSETEQIGKLFETTMLKKYGPTELNEHFMSFNTICDATQERQ 301

Query: 355 DAMYKLVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNH 414
           DAM  LVE ++D+M+V+GG+NSSNT+HLQEI+ E+GI SY IDS++RI PGN+I +K   
Sbjct: 302 DAMLNLVEEEVDVMVVIGGFNSSNTTHLQEISIEKGIDSYHIDSDKRILPGNKIEHKPLE 361

Query: 415 GELVEKENWLPKGHITIGVTSGASTPDKVVEDALIKVFDIKREEAL 461
            ++  KENWLP G I +GVTSGASTPDKVVE  + ++F+ K    L
Sbjct: 362 KDIETKENWLPDGKIIVGVTSGASTPDKVVEAVIERIFEEKNAGVL 401

BLAST of Chy1G000040 vs. ExPASy Swiss-Prot
Match: Q31S64 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Synechococcus elongatus (strain PCC 7942 / FACHB-805) OX=1140 GN=ispH PE=3 SV=1)

HSP 1 Score: 530.4 bits (1365), Expect = 2.0e-149
Identity = 253/402 (62.94%), Postives = 315/402 (78.36%), Query Frame = 0

Query: 55  DAKVFRRNLTKSDNYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYTWGDVTVKLAE 114
           D K F+R L +SD YNRKGFG   +    +   Y SD+I++L++NG+    G++T++LAE
Sbjct: 2   DTKAFKRALHQSDRYNRKGFGKTTDVSGALESAYQSDLIQSLRQNGYRLQRGEITIRLAE 61

Query: 115 AYGFCWGVERAVQIAYEARKQFPDEKIWLTNEIIHNPTVNKRLEEMEVQNIPLEEGRKQF 174
           A+GFCWGVERAV IAYE R+ FP E+IW+TNEIIHNP+VN+ L EM V+ IP E G K F
Sbjct: 62  AFGFCWGVERAVAIAYETRQHFPQERIWITNEIIHNPSVNQHLREMSVEFIPCERGEKDF 121

Query: 175 EVVDKGDVVILPAFGAAVDEMLTLSEKKVQIVDTTCPWVSKVWNVVDKHKKGDYTSIIHG 234
            VVD+GDVVILPAFGA+V EM  L EK   IVDTTCPWVSKVWN V+KHK+G +TSIIHG
Sbjct: 122 SVVDRGDVVILPAFGASVQEMQLLDEKGCHIVDTTCPWVSKVWNTVEKHKRGAHTSIIHG 181

Query: 235 KYAHEETVATASFAGTYIIVKNMAEAIYVCDYILGGELDGSSSTKGEFMKKFKNAVSKGF 294
           KY HEETVAT+SFA TY++V N+ +A YVCDYIL G        + EFM +F  A S GF
Sbjct: 182 KYNHEETVATSSFAETYLVVLNLEQAQYVCDYILNG------GDRDEFMTRFGKACSAGF 241

Query: 295 DPDVDLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDATQERQ 354
           DPD DL ++GIANQTTMLK ETE IGKL ERT+++K+G + +N+HFL+FNTICDATQERQ
Sbjct: 242 DPDRDLERIGIANQTTMLKSETEAIGKLFERTLLKKYGPQALNDHFLAFNTICDATQERQ 301

Query: 355 DAMYKLVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNH 414
           DAM++LVE  LDL++V+GG+NSSNT+HLQEIA ER IPS+ ID+ +RIGPGNRI +K  H
Sbjct: 302 DAMFQLVEEPLDLIVVIGGFNSSNTTHLQEIAIERQIPSFHIDAAERIGPGNRIEHKPLH 361

Query: 415 GELVEKENWLPKGHITIGVTSGASTPDKVVEDALIKVFDIKR 457
            +L   E WLP G +TIG+TSGASTPDKVVED + ++FD++R
Sbjct: 362 TDLTTTEPWLPAGPLTIGITSGASTPDKVVEDVIERLFDLQR 397

BLAST of Chy1G000040 vs. ExPASy Swiss-Prot
Match: Q5N249 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) OX=269084 GN=ispH PE=3 SV=1)

HSP 1 Score: 530.4 bits (1365), Expect = 2.0e-149
Identity = 253/402 (62.94%), Postives = 315/402 (78.36%), Query Frame = 0

Query: 55  DAKVFRRNLTKSDNYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYTWGDVTVKLAE 114
           D K F+R L +SD YNRKGFG   +    +   Y SD+I++L++NG+    G++T++LAE
Sbjct: 2   DTKAFKRALHQSDRYNRKGFGKTTDVSGALESAYQSDLIQSLRQNGYRLQRGEITIRLAE 61

Query: 115 AYGFCWGVERAVQIAYEARKQFPDEKIWLTNEIIHNPTVNKRLEEMEVQNIPLEEGRKQF 174
           A+GFCWGVERAV IAYE R+ FP E+IW+TNEIIHNP+VN+ L EM V+ IP E G K F
Sbjct: 62  AFGFCWGVERAVAIAYETRQHFPQERIWITNEIIHNPSVNQHLREMSVEFIPCERGEKDF 121

Query: 175 EVVDKGDVVILPAFGAAVDEMLTLSEKKVQIVDTTCPWVSKVWNVVDKHKKGDYTSIIHG 234
            VVD+GDVVILPAFGA+V EM  L EK   IVDTTCPWVSKVWN V+KHK+G +TSIIHG
Sbjct: 122 SVVDRGDVVILPAFGASVQEMQLLDEKGCHIVDTTCPWVSKVWNTVEKHKRGAHTSIIHG 181

Query: 235 KYAHEETVATASFAGTYIIVKNMAEAIYVCDYILGGELDGSSSTKGEFMKKFKNAVSKGF 294
           KY HEETVAT+SFA TY++V N+ +A YVCDYIL G        + EFM +F  A S GF
Sbjct: 182 KYNHEETVATSSFAETYLVVLNLEQAQYVCDYILNG------GDRDEFMTRFGKACSAGF 241

Query: 295 DPDVDLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDATQERQ 354
           DPD DL ++GIANQTTMLK ETE IGKL ERT+++K+G + +N+HFL+FNTICDATQERQ
Sbjct: 242 DPDRDLERIGIANQTTMLKSETEAIGKLFERTLLKKYGPQALNDHFLAFNTICDATQERQ 301

Query: 355 DAMYKLVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNH 414
           DAM++LVE  LDL++V+GG+NSSNT+HLQEIA ER IPS+ ID+ +RIGPGNRI +K  H
Sbjct: 302 DAMFQLVEEPLDLIVVIGGFNSSNTTHLQEIAIERQIPSFHIDAAERIGPGNRIEHKPLH 361

Query: 415 GELVEKENWLPKGHITIGVTSGASTPDKVVEDALIKVFDIKR 457
            +L   E WLP G +TIG+TSGASTPDKVVED + ++FD++R
Sbjct: 362 TDLTTTEPWLPAGPLTIGITSGASTPDKVVEDVIERLFDLQR 397

BLAST of Chy1G000040 vs. ExPASy TrEMBL
Match: A0A0A0K3D8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G075680 PE=3 SV=1)

HSP 1 Score: 920.6 bits (2378), Expect = 2.6e-264
Identity = 458/468 (97.86%), Postives = 462/468 (98.72%), Query Frame = 0

Query: 1   MAFSMQLYRLPTRTDAAMPENFTAVRSLRFRKPMTVRCDGD-----SSSSSSAAVDSNFD 60
           MAFSMQLYRLPTRTDAAMPENFTAVRSLR+RKPM+VRCDGD     SSSSSSAAVDSNFD
Sbjct: 1   MAFSMQLYRLPTRTDAAMPENFTAVRSLRYRKPMSVRCDGDSSSSSSSSSSSAAVDSNFD 60

Query: 61  AKVFRRNLTKSDNYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYTWGDVTVKLAEA 120
           AKVFRRNLTKSDNYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYTWGDVTVKLAEA
Sbjct: 61  AKVFRRNLTKSDNYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYTWGDVTVKLAEA 120

Query: 121 YGFCWGVERAVQIAYEARKQFPDEKIWLTNEIIHNPTVNKRLEEMEVQNIPLEEGRKQFE 180
           YGFCWGVERAVQIAYEARKQFPDEKIWLTNEIIHNPTVNKRLEEMEVQNIPLE+GRKQFE
Sbjct: 121 YGFCWGVERAVQIAYEARKQFPDEKIWLTNEIIHNPTVNKRLEEMEVQNIPLEDGRKQFE 180

Query: 181 VVDKGDVVILPAFGAAVDEMLTLSEKKVQIVDTTCPWVSKVWNVVDKHKKGDYTSIIHGK 240
           VVDKGDVVILPAFGAAVDEMLTLSEKKVQIVDTTCPWVSKVWNVVDKHKKGDYTSIIHGK
Sbjct: 181 VVDKGDVVILPAFGAAVDEMLTLSEKKVQIVDTTCPWVSKVWNVVDKHKKGDYTSIIHGK 240

Query: 241 YAHEETVATASFAGTYIIVKNMAEAIYVCDYILGGELDGSSSTKGEFMKKFKNAVSKGFD 300
           YAHEETVATASFAGT+IIVKNMAEAIYVCDYILGGELDGSSSTK EFMKKFKNAVSKGFD
Sbjct: 241 YAHEETVATASFAGTFIIVKNMAEAIYVCDYILGGELDGSSSTKEEFMKKFKNAVSKGFD 300

Query: 301 PDVDLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDATQERQD 360
           PDVDLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDATQERQD
Sbjct: 301 PDVDLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDATQERQD 360

Query: 361 AMYKLVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHG 420
           AMYKLVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHG
Sbjct: 361 AMYKLVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHG 420

Query: 421 ELVEKENWLPKGHITIGVTSGASTPDKVVEDALIKVFDIKREEALQFA 464
           ELVEKENWLPKGHITIGVTSGASTPDKVVEDALIKVFDIKREEALQFA
Sbjct: 421 ELVEKENWLPKGHITIGVTSGASTPDKVVEDALIKVFDIKREEALQFA 468

BLAST of Chy1G000040 vs. ExPASy TrEMBL
Match: A0A5A7SJM1 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G00040 PE=3 SV=1)

HSP 1 Score: 916.8 bits (2368), Expect = 3.7e-263
Identity = 453/463 (97.84%), Postives = 460/463 (99.35%), Query Frame = 0

Query: 1   MAFSMQLYRLPTRTDAAMPENFTAVRSLRFRKPMTVRCDGDSSSSSSAAVDSNFDAKVFR 60
           MAFSMQLYRLPTRTDAA+PEN +AVRSLR+RKPM+VRCDGDSSSSSSAAVDS+FDAKVFR
Sbjct: 1   MAFSMQLYRLPTRTDAALPENSSAVRSLRYRKPMSVRCDGDSSSSSSAAVDSDFDAKVFR 60

Query: 61  RNLTKSDNYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYTWGDVTVKLAEAYGFCW 120
           RNLTKSDNYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYTWGDVTVKLAEAYGFCW
Sbjct: 61  RNLTKSDNYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYTWGDVTVKLAEAYGFCW 120

Query: 121 GVERAVQIAYEARKQFPDEKIWLTNEIIHNPTVNKRLEEMEVQNIPLEEGRKQFEVVDKG 180
           GVERAVQIAYEARKQFPDEKIWLTNEIIHNPTVNKRLEEMEVQNIPLEEGRKQFEVVDKG
Sbjct: 121 GVERAVQIAYEARKQFPDEKIWLTNEIIHNPTVNKRLEEMEVQNIPLEEGRKQFEVVDKG 180

Query: 181 DVVILPAFGAAVDEMLTLSEKKVQIVDTTCPWVSKVWNVVDKHKKGDYTSIIHGKYAHEE 240
           DVVILPAFGAAVDEMLTLSEKKVQIVDTTCPWVSKVWNVVDKHKKGDYTSIIHGKYAHEE
Sbjct: 181 DVVILPAFGAAVDEMLTLSEKKVQIVDTTCPWVSKVWNVVDKHKKGDYTSIIHGKYAHEE 240

Query: 241 TVATASFAGTYIIVKNMAEAIYVCDYILGGELDGSSSTKGEFMKKFKNAVSKGFDPDVDL 300
           TVATASFAG YIIVKNMAEA+YVCDYILGGELDGSSSTK EFMKKFKNAVS+GFDPDVDL
Sbjct: 241 TVATASFAGKYIIVKNMAEAMYVCDYILGGELDGSSSTKEEFMKKFKNAVSEGFDPDVDL 300

Query: 301 LKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDATQERQDAMYKL 360
           LKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDATQERQDAMYKL
Sbjct: 301 LKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDATQERQDAMYKL 360

Query: 361 VEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHGELVEK 420
           VEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHGELVEK
Sbjct: 361 VEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHGELVEK 420

Query: 421 ENWLPKGHITIGVTSGASTPDKVVEDALIKVFDIKREEALQFA 464
           ENWLPKGHITIGVTSGASTPDKVVEDALIKVFDIKREEALQFA
Sbjct: 421 ENWLPKGHITIGVTSGASTPDKVVEDALIKVFDIKREEALQFA 463

BLAST of Chy1G000040 vs. ExPASy TrEMBL
Match: A0A1S3BZP5 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103495189 PE=3 SV=1)

HSP 1 Score: 916.8 bits (2368), Expect = 3.7e-263
Identity = 453/463 (97.84%), Postives = 460/463 (99.35%), Query Frame = 0

Query: 1   MAFSMQLYRLPTRTDAAMPENFTAVRSLRFRKPMTVRCDGDSSSSSSAAVDSNFDAKVFR 60
           MAFSMQLYRLPTRTDAA+PEN +AVRSLR+RKPM+VRCDGDSSSSSSAAVDS+FDAKVFR
Sbjct: 1   MAFSMQLYRLPTRTDAALPENSSAVRSLRYRKPMSVRCDGDSSSSSSAAVDSDFDAKVFR 60

Query: 61  RNLTKSDNYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYTWGDVTVKLAEAYGFCW 120
           RNLTKSDNYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYTWGDVTVKLAEAYGFCW
Sbjct: 61  RNLTKSDNYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYTWGDVTVKLAEAYGFCW 120

Query: 121 GVERAVQIAYEARKQFPDEKIWLTNEIIHNPTVNKRLEEMEVQNIPLEEGRKQFEVVDKG 180
           GVERAVQIAYEARKQFPDEKIWLTNEIIHNPTVNKRLEEMEVQNIPLEEGRKQFEVVDKG
Sbjct: 121 GVERAVQIAYEARKQFPDEKIWLTNEIIHNPTVNKRLEEMEVQNIPLEEGRKQFEVVDKG 180

Query: 181 DVVILPAFGAAVDEMLTLSEKKVQIVDTTCPWVSKVWNVVDKHKKGDYTSIIHGKYAHEE 240
           DVVILPAFGAAVDEMLTLSEKKVQIVDTTCPWVSKVWNVVDKHKKGDYTSIIHGKYAHEE
Sbjct: 181 DVVILPAFGAAVDEMLTLSEKKVQIVDTTCPWVSKVWNVVDKHKKGDYTSIIHGKYAHEE 240

Query: 241 TVATASFAGTYIIVKNMAEAIYVCDYILGGELDGSSSTKGEFMKKFKNAVSKGFDPDVDL 300
           TVATASFAG YIIVKNMAEA+YVCDYILGGELDGSSSTK EFMKKFKNAVS+GFDPDVDL
Sbjct: 241 TVATASFAGKYIIVKNMAEAMYVCDYILGGELDGSSSTKEEFMKKFKNAVSEGFDPDVDL 300

Query: 301 LKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDATQERQDAMYKL 360
           LKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDATQERQDAMYKL
Sbjct: 301 LKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDATQERQDAMYKL 360

Query: 361 VEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHGELVEK 420
           VEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHGELVEK
Sbjct: 361 VEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHGELVEK 420

Query: 421 ENWLPKGHITIGVTSGASTPDKVVEDALIKVFDIKREEALQFA 464
           ENWLPKGHITIGVTSGASTPDKVVEDALIKVFDIKREEALQFA
Sbjct: 421 ENWLPKGHITIGVTSGASTPDKVVEDALIKVFDIKREEALQFA 463

BLAST of Chy1G000040 vs. ExPASy TrEMBL
Match: A0A6J1I4K4 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111470927 PE=3 SV=1)

HSP 1 Score: 869.8 bits (2246), Expect = 5.2e-249
Identity = 429/468 (91.67%), Postives = 449/468 (95.94%), Query Frame = 0

Query: 1   MAFSMQLYRLPTRTDAAMPENFTAVRSLRFRKPMTVRCDGD-----SSSSSSAAVDSNFD 60
           MAFSMQLYRL TRTDAA+PE+F  VRS+R RKP++VRCDGD     SSSSSS AVDSNFD
Sbjct: 1   MAFSMQLYRLATRTDAALPESFPGVRSVRCRKPLSVRCDGDSSASASSSSSSVAVDSNFD 60

Query: 61  AKVFRRNLTKSDNYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYTWGDVTVKLAEA 120
           AKVFRRNL+KS+NYNR+GFG+KDETLALMN EYTSDIIKTLKENGFEYTWGDVTVKLAEA
Sbjct: 61  AKVFRRNLSKSENYNRRGFGHKDETLALMNSEYTSDIIKTLKENGFEYTWGDVTVKLAEA 120

Query: 121 YGFCWGVERAVQIAYEARKQFPDEKIWLTNEIIHNPTVNKRLEEMEVQNIPLEEGRKQFE 180
           YGFCWGVERAVQIAYEARKQFPDEK+WLTNEIIHNPTVNKRLEEMEVQNIPLE+G+KQFE
Sbjct: 121 YGFCWGVERAVQIAYEARKQFPDEKLWLTNEIIHNPTVNKRLEEMEVQNIPLEDGKKQFE 180

Query: 181 VVDKGDVVILPAFGAAVDEMLTLSEKKVQIVDTTCPWVSKVWNVVDKHKKGDYTSIIHGK 240
           VV KGDVVILPAFGA VDEMLTLSEKKVQIVDTTCPWVSKVWNVV+KHKKG++TSIIHGK
Sbjct: 181 VVGKGDVVILPAFGAGVDEMLTLSEKKVQIVDTTCPWVSKVWNVVEKHKKGEFTSIIHGK 240

Query: 241 YAHEETVATASFAGTYIIVKNMAEAIYVCDYILGGELDGSSSTKGEFMKKFKNAVSKGFD 300
           Y+HEETVATASFAG YIIVKNMAEA YVCDYILGGELDGSSSTK EFMKKFKNAVS+GFD
Sbjct: 241 YSHEETVATASFAGKYIIVKNMAEATYVCDYILGGELDGSSSTKEEFMKKFKNAVSQGFD 300

Query: 301 PDVDLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDATQERQD 360
           PD DLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDATQERQD
Sbjct: 301 PDTDLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDATQERQD 360

Query: 361 AMYKLVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHG 420
           AMYKLVEGK+DLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHG
Sbjct: 361 AMYKLVEGKVDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHG 420

Query: 421 ELVEKENWLPKGHITIGVTSGASTPDKVVEDALIKVFDIKREEALQFA 464
           ELVE+ENWLPKG ITIGVTSGASTPDKVVED LIKVFDIKRE+ALQFA
Sbjct: 421 ELVERENWLPKGPITIGVTSGASTPDKVVEDVLIKVFDIKREQALQFA 468

BLAST of Chy1G000040 vs. ExPASy TrEMBL
Match: K7NBR0 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Siraitia grosvenorii OX=190515 PE=2 SV=1)

HSP 1 Score: 869.0 bits (2244), Expect = 8.9e-249
Identity = 423/470 (90.00%), Postives = 453/470 (96.38%), Query Frame = 0

Query: 1   MAFSMQLYRLPTRTDAAMPENFTAVRSLRFRKPMTVRCDGDSSSSSSA-------AVDSN 60
           MAFS+QLYRL TRTDAA+PENF+ +R++R+RKP++VRCDGDSSSSSS+       A+DSN
Sbjct: 1   MAFSLQLYRLSTRTDAALPENFSGIRAVRYRKPLSVRCDGDSSSSSSSSSSSSSVAMDSN 60

Query: 61  FDAKVFRRNLTKSDNYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYTWGDVTVKLA 120
           FDAKVFR+NLT+S+NYNRKGFGYKDETLALMNREYTSDIIKTLKE+ +EYTWGDVTVKLA
Sbjct: 61  FDAKVFRKNLTRSENYNRKGFGYKDETLALMNREYTSDIIKTLKEHEYEYTWGDVTVKLA 120

Query: 121 EAYGFCWGVERAVQIAYEARKQFPDEKIWLTNEIIHNPTVNKRLEEMEVQNIPLEEGRKQ 180
           EAYGFCWGVERAVQIAYEARKQFPDEKIW+TNEIIHNPTVNKRLEEMEV+NIPLEEG+KQ
Sbjct: 121 EAYGFCWGVERAVQIAYEARKQFPDEKIWITNEIIHNPTVNKRLEEMEVENIPLEEGKKQ 180

Query: 181 FEVVDKGDVVILPAFGAAVDEMLTLSEKKVQIVDTTCPWVSKVWNVVDKHKKGDYTSIIH 240
           FEVV+KGDVVILPAFGAAVDEMLTLSEK VQIVDTTCPWVSKVWNVV+KHKKGD+TSIIH
Sbjct: 181 FEVVNKGDVVILPAFGAAVDEMLTLSEKNVQIVDTTCPWVSKVWNVVEKHKKGDFTSIIH 240

Query: 241 GKYAHEETVATASFAGTYIIVKNMAEAIYVCDYILGGELDGSSSTKGEFMKKFKNAVSKG 300
           GKYAHEETVATASFAG YIIVKNMAEA YVCDYILGGELDGSS TK EFMKKFKNAVSKG
Sbjct: 241 GKYAHEETVATASFAGKYIIVKNMAEATYVCDYILGGELDGSSPTKEEFMKKFKNAVSKG 300

Query: 301 FDPDVDLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDATQER 360
           FDPD+DL+KVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHF+SFNTICDATQER
Sbjct: 301 FDPDIDLVKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFISFNTICDATQER 360

Query: 361 QDAMYKLVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLN 420
           QDAMYKLVEGKLDLMLVVGGWNSSNTSHLQ+IAE+RGIPSYW+DSEQRIGPGN+IAYKLN
Sbjct: 361 QDAMYKLVEGKLDLMLVVGGWNSSNTSHLQQIAEDRGIPSYWVDSEQRIGPGNKIAYKLN 420

Query: 421 HGELVEKENWLPKGHITIGVTSGASTPDKVVEDALIKVFDIKREEALQFA 464
           HGELVEKENWLP+G ITIGVTSGASTPDKVVED LIKVFDIKREEALQFA
Sbjct: 421 HGELVEKENWLPRGPITIGVTSGASTPDKVVEDVLIKVFDIKREEALQFA 470

BLAST of Chy1G000040 vs. NCBI nr
Match: XP_004137008.2 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic [Cucumis sativus])

HSP 1 Score: 915 bits (2366), Expect = 0.0
Identity = 458/468 (97.86%), Postives = 462/468 (98.72%), Query Frame = 0

Query: 1   MAFSMQLYRLPTRTDAAMPENFTAVRSLRFRKPMTVRCDGDSSSSSS-----AAVDSNFD 60
           MAFSMQLYRLPTRTDAAMPENFTAVRSLR+RKPM+VRCDGDSSSSSS     AAVDSNFD
Sbjct: 1   MAFSMQLYRLPTRTDAAMPENFTAVRSLRYRKPMSVRCDGDSSSSSSSSSSSAAVDSNFD 60

Query: 61  AKVFRRNLTKSDNYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYTWGDVTVKLAEA 120
           AKVFRRNLTKSDNYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYTWGDVTVKLAEA
Sbjct: 61  AKVFRRNLTKSDNYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYTWGDVTVKLAEA 120

Query: 121 YGFCWGVERAVQIAYEARKQFPDEKIWLTNEIIHNPTVNKRLEEMEVQNIPLEEGRKQFE 180
           YGFCWGVERAVQIAYEARKQFPDEKIWLTNEIIHNPTVNKRLEEMEVQNIPLE+GRKQFE
Sbjct: 121 YGFCWGVERAVQIAYEARKQFPDEKIWLTNEIIHNPTVNKRLEEMEVQNIPLEDGRKQFE 180

Query: 181 VVDKGDVVILPAFGAAVDEMLTLSEKKVQIVDTTCPWVSKVWNVVDKHKKGDYTSIIHGK 240
           VVDKGDVVILPAFGAAVDEMLTLSEKKVQIVDTTCPWVSKVWNVVDKHKKGDYTSIIHGK
Sbjct: 181 VVDKGDVVILPAFGAAVDEMLTLSEKKVQIVDTTCPWVSKVWNVVDKHKKGDYTSIIHGK 240

Query: 241 YAHEETVATASFAGTYIIVKNMAEAIYVCDYILGGELDGSSSTKGEFMKKFKNAVSKGFD 300
           YAHEETVATASFAGT+IIVKNMAEAIYVCDYILGGELDGSSSTK EFMKKFKNAVSKGFD
Sbjct: 241 YAHEETVATASFAGTFIIVKNMAEAIYVCDYILGGELDGSSSTKEEFMKKFKNAVSKGFD 300

Query: 301 PDVDLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDATQERQD 360
           PDVDLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDATQERQD
Sbjct: 301 PDVDLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDATQERQD 360

Query: 361 AMYKLVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHG 420
           AMYKLVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHG
Sbjct: 361 AMYKLVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHG 420

Query: 421 ELVEKENWLPKGHITIGVTSGASTPDKVVEDALIKVFDIKREEALQFA 463
           ELVEKENWLPKGHITIGVTSGASTPDKVVEDALIKVFDIKREEALQFA
Sbjct: 421 ELVEKENWLPKGHITIGVTSGASTPDKVVEDALIKVFDIKREEALQFA 468

BLAST of Chy1G000040 vs. NCBI nr
Match: KAE8645891.1 (hypothetical protein Csa_017704 [Cucumis sativus])

HSP 1 Score: 915 bits (2366), Expect = 0.0
Identity = 458/468 (97.86%), Postives = 462/468 (98.72%), Query Frame = 0

Query: 1   MAFSMQLYRLPTRTDAAMPENFTAVRSLRFRKPMTVRCDGDSSSSSS-----AAVDSNFD 60
           MAFSMQLYRLPTRTDAAMPENFTAVRSLR+RKPM+VRCDGDSSSSSS     AAVDSNFD
Sbjct: 66  MAFSMQLYRLPTRTDAAMPENFTAVRSLRYRKPMSVRCDGDSSSSSSSSSSSAAVDSNFD 125

Query: 61  AKVFRRNLTKSDNYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYTWGDVTVKLAEA 120
           AKVFRRNLTKSDNYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYTWGDVTVKLAEA
Sbjct: 126 AKVFRRNLTKSDNYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYTWGDVTVKLAEA 185

Query: 121 YGFCWGVERAVQIAYEARKQFPDEKIWLTNEIIHNPTVNKRLEEMEVQNIPLEEGRKQFE 180
           YGFCWGVERAVQIAYEARKQFPDEKIWLTNEIIHNPTVNKRLEEMEVQNIPLE+GRKQFE
Sbjct: 186 YGFCWGVERAVQIAYEARKQFPDEKIWLTNEIIHNPTVNKRLEEMEVQNIPLEDGRKQFE 245

Query: 181 VVDKGDVVILPAFGAAVDEMLTLSEKKVQIVDTTCPWVSKVWNVVDKHKKGDYTSIIHGK 240
           VVDKGDVVILPAFGAAVDEMLTLSEKKVQIVDTTCPWVSKVWNVVDKHKKGDYTSIIHGK
Sbjct: 246 VVDKGDVVILPAFGAAVDEMLTLSEKKVQIVDTTCPWVSKVWNVVDKHKKGDYTSIIHGK 305

Query: 241 YAHEETVATASFAGTYIIVKNMAEAIYVCDYILGGELDGSSSTKGEFMKKFKNAVSKGFD 300
           YAHEETVATASFAGT+IIVKNMAEAIYVCDYILGGELDGSSSTK EFMKKFKNAVSKGFD
Sbjct: 306 YAHEETVATASFAGTFIIVKNMAEAIYVCDYILGGELDGSSSTKEEFMKKFKNAVSKGFD 365

Query: 301 PDVDLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDATQERQD 360
           PDVDLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDATQERQD
Sbjct: 366 PDVDLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDATQERQD 425

Query: 361 AMYKLVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHG 420
           AMYKLVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHG
Sbjct: 426 AMYKLVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHG 485

Query: 421 ELVEKENWLPKGHITIGVTSGASTPDKVVEDALIKVFDIKREEALQFA 463
           ELVEKENWLPKGHITIGVTSGASTPDKVVEDALIKVFDIKREEALQFA
Sbjct: 486 ELVEKENWLPKGHITIGVTSGASTPDKVVEDALIKVFDIKREEALQFA 533

BLAST of Chy1G000040 vs. NCBI nr
Match: XP_008454877.1 (PREDICTED: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like [Cucumis melo] >KAA0031252.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase [Cucumis melo var. makuwa] >TYK06703.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase [Cucumis melo var. makuwa])

HSP 1 Score: 912 bits (2356), Expect = 0.0
Identity = 453/463 (97.84%), Postives = 460/463 (99.35%), Query Frame = 0

Query: 1   MAFSMQLYRLPTRTDAAMPENFTAVRSLRFRKPMTVRCDGDSSSSSSAAVDSNFDAKVFR 60
           MAFSMQLYRLPTRTDAA+PEN +AVRSLR+RKPM+VRCDGDSSSSSSAAVDS+FDAKVFR
Sbjct: 1   MAFSMQLYRLPTRTDAALPENSSAVRSLRYRKPMSVRCDGDSSSSSSAAVDSDFDAKVFR 60

Query: 61  RNLTKSDNYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYTWGDVTVKLAEAYGFCW 120
           RNLTKSDNYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYTWGDVTVKLAEAYGFCW
Sbjct: 61  RNLTKSDNYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYTWGDVTVKLAEAYGFCW 120

Query: 121 GVERAVQIAYEARKQFPDEKIWLTNEIIHNPTVNKRLEEMEVQNIPLEEGRKQFEVVDKG 180
           GVERAVQIAYEARKQFPDEKIWLTNEIIHNPTVNKRLEEMEVQNIPLEEGRKQFEVVDKG
Sbjct: 121 GVERAVQIAYEARKQFPDEKIWLTNEIIHNPTVNKRLEEMEVQNIPLEEGRKQFEVVDKG 180

Query: 181 DVVILPAFGAAVDEMLTLSEKKVQIVDTTCPWVSKVWNVVDKHKKGDYTSIIHGKYAHEE 240
           DVVILPAFGAAVDEMLTLSEKKVQIVDTTCPWVSKVWNVVDKHKKGDYTSIIHGKYAHEE
Sbjct: 181 DVVILPAFGAAVDEMLTLSEKKVQIVDTTCPWVSKVWNVVDKHKKGDYTSIIHGKYAHEE 240

Query: 241 TVATASFAGTYIIVKNMAEAIYVCDYILGGELDGSSSTKGEFMKKFKNAVSKGFDPDVDL 300
           TVATASFAG YIIVKNMAEA+YVCDYILGGELDGSSSTK EFMKKFKNAVS+GFDPDVDL
Sbjct: 241 TVATASFAGKYIIVKNMAEAMYVCDYILGGELDGSSSTKEEFMKKFKNAVSEGFDPDVDL 300

Query: 301 LKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDATQERQDAMYKL 360
           LKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDATQERQDAMYKL
Sbjct: 301 LKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDATQERQDAMYKL 360

Query: 361 VEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHGELVEK 420
           VEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHGELVEK
Sbjct: 361 VEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHGELVEK 420

Query: 421 ENWLPKGHITIGVTSGASTPDKVVEDALIKVFDIKREEALQFA 463
           ENWLPKGHITIGVTSGASTPDKVVEDALIKVFDIKREEALQFA
Sbjct: 421 ENWLPKGHITIGVTSGASTPDKVVEDALIKVFDIKREEALQFA 463

BLAST of Chy1G000040 vs. NCBI nr
Match: XP_038888882.1 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic [Benincasa hispida])

HSP 1 Score: 889 bits (2298), Expect = 0.0
Identity = 440/463 (95.03%), Postives = 455/463 (98.27%), Query Frame = 0

Query: 1   MAFSMQLYRLPTRTDAAMPENFTAVRSLRFRKPMTVRCDGDSSSSSSAAVDSNFDAKVFR 60
           MAFSMQLYRLPTRTDA +PENF  +RS+R+RKP++VRCDGDSSSS  AAVDS+FDAKVFR
Sbjct: 1   MAFSMQLYRLPTRTDALLPENFATLRSVRYRKPLSVRCDGDSSSS--AAVDSDFDAKVFR 60

Query: 61  RNLTKSDNYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYTWGDVTVKLAEAYGFCW 120
           RNLTKS+NYNR+GFGYKDETLALMNREYTSD+IKTLKENGFEYTWG+VTVKLAEAYGFCW
Sbjct: 61  RNLTKSENYNRRGFGYKDETLALMNREYTSDVIKTLKENGFEYTWGNVTVKLAEAYGFCW 120

Query: 121 GVERAVQIAYEARKQFPDEKIWLTNEIIHNPTVNKRLEEMEVQNIPLEEGRKQFEVVDKG 180
           GVERAVQIAYEARKQFPDEKIWLTNEIIHNPTVNKRLEEMEVQNIPLEEGRKQFEVVDKG
Sbjct: 121 GVERAVQIAYEARKQFPDEKIWLTNEIIHNPTVNKRLEEMEVQNIPLEEGRKQFEVVDKG 180

Query: 181 DVVILPAFGAAVDEMLTLSEKKVQIVDTTCPWVSKVWNVVDKHKKGDYTSIIHGKYAHEE 240
           DVVILPAFGAAV+EMLTLSEKKVQIVDTTCPWVSKVWNVVDKHKKGDYTSIIHGKYAHEE
Sbjct: 181 DVVILPAFGAAVEEMLTLSEKKVQIVDTTCPWVSKVWNVVDKHKKGDYTSIIHGKYAHEE 240

Query: 241 TVATASFAGTYIIVKNMAEAIYVCDYILGGELDGSSSTKGEFMKKFKNAVSKGFDPDVDL 300
           TVATASFAG YIIVKNMAEA+YVCDYILGGELDGSSSTK EFMKKFKNAVS+GFDPD+DL
Sbjct: 241 TVATASFAGKYIIVKNMAEAMYVCDYILGGELDGSSSTKEEFMKKFKNAVSEGFDPDIDL 300

Query: 301 LKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDATQERQDAMYKL 360
           LKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDATQERQDAMYKL
Sbjct: 301 LKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDATQERQDAMYKL 360

Query: 361 VEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHGELVEK 420
           VEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHGELVEK
Sbjct: 361 VEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHGELVEK 420

Query: 421 ENWLPKGHITIGVTSGASTPDKVVEDALIKVFDIKREEALQFA 463
           ENWLPKG ITIGVTSGASTPDKVVEDALIKVFDIKREEALQFA
Sbjct: 421 ENWLPKGPITIGVTSGASTPDKVVEDALIKVFDIKREEALQFA 461

BLAST of Chy1G000040 vs. NCBI nr
Match: XP_022972347.1 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic [Cucurbita maxima])

HSP 1 Score: 865 bits (2234), Expect = 1.64e-315
Identity = 429/468 (91.67%), Postives = 449/468 (95.94%), Query Frame = 0

Query: 1   MAFSMQLYRLPTRTDAAMPENFTAVRSLRFRKPMTVRCDGDSS-----SSSSAAVDSNFD 60
           MAFSMQLYRL TRTDAA+PE+F  VRS+R RKP++VRCDGDSS     SSSS AVDSNFD
Sbjct: 1   MAFSMQLYRLATRTDAALPESFPGVRSVRCRKPLSVRCDGDSSASASSSSSSVAVDSNFD 60

Query: 61  AKVFRRNLTKSDNYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYTWGDVTVKLAEA 120
           AKVFRRNL+KS+NYNR+GFG+KDETLALMN EYTSDIIKTLKENGFEYTWGDVTVKLAEA
Sbjct: 61  AKVFRRNLSKSENYNRRGFGHKDETLALMNSEYTSDIIKTLKENGFEYTWGDVTVKLAEA 120

Query: 121 YGFCWGVERAVQIAYEARKQFPDEKIWLTNEIIHNPTVNKRLEEMEVQNIPLEEGRKQFE 180
           YGFCWGVERAVQIAYEARKQFPDEK+WLTNEIIHNPTVNKRLEEMEVQNIPLE+G+KQFE
Sbjct: 121 YGFCWGVERAVQIAYEARKQFPDEKLWLTNEIIHNPTVNKRLEEMEVQNIPLEDGKKQFE 180

Query: 181 VVDKGDVVILPAFGAAVDEMLTLSEKKVQIVDTTCPWVSKVWNVVDKHKKGDYTSIIHGK 240
           VV KGDVVILPAFGA VDEMLTLSEKKVQIVDTTCPWVSKVWNVV+KHKKG++TSIIHGK
Sbjct: 181 VVGKGDVVILPAFGAGVDEMLTLSEKKVQIVDTTCPWVSKVWNVVEKHKKGEFTSIIHGK 240

Query: 241 YAHEETVATASFAGTYIIVKNMAEAIYVCDYILGGELDGSSSTKGEFMKKFKNAVSKGFD 300
           Y+HEETVATASFAG YIIVKNMAEA YVCDYILGGELDGSSSTK EFMKKFKNAVS+GFD
Sbjct: 241 YSHEETVATASFAGKYIIVKNMAEATYVCDYILGGELDGSSSTKEEFMKKFKNAVSQGFD 300

Query: 301 PDVDLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDATQERQD 360
           PD DLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDATQERQD
Sbjct: 301 PDTDLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDATQERQD 360

Query: 361 AMYKLVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHG 420
           AMYKLVEGK+DLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHG
Sbjct: 361 AMYKLVEGKVDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHG 420

Query: 421 ELVEKENWLPKGHITIGVTSGASTPDKVVEDALIKVFDIKREEALQFA 463
           ELVE+ENWLPKG ITIGVTSGASTPDKVVED LIKVFDIKRE+ALQFA
Sbjct: 421 ELVERENWLPKGPITIGVTSGASTPDKVVEDVLIKVFDIKREQALQFA 468

BLAST of Chy1G000040 vs. TAIR 10
Match: AT4G34350.1 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase )

HSP 1 Score: 738.4 bits (1905), Expect = 3.5e-213
Identity = 359/466 (77.04%), Postives = 417/466 (89.48%), Query Frame = 0

Query: 1   MAFSMQLYRLPTRTDAAMPEN-FTAVRSLRFRKPMTVRCDG--DSSSSSSAAVDSNFDAK 60
           MA ++Q  RL  R D  + EN  +   SLR RK ++VRC    +++ S S  +DS+FDAK
Sbjct: 1   MAVALQFSRLCVRPDTFVRENHLSGSGSLRRRKALSVRCSSGDENAPSPSVVMDSDFDAK 60

Query: 61  VFRRNLTKSDNYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYTWGDVTVKLAEAYG 120
           VFR+NLT+SDNYNRKGFG+K+ETL LMNREYTSDI++TLK NG+ Y+WGDVTVKLA+AYG
Sbjct: 61  VFRKNLTRSDNYNRKGFGHKEETLKLMNREYTSDILETLKTNGYTYSWGDVTVKLAKAYG 120

Query: 121 FCWGVERAVQIAYEARKQFPDEKIWLTNEIIHNPTVNKRLEEMEVQNIPLEEGRKQFEVV 180
           FCWGVERAVQIAYEARKQFP+E++W+TNEIIHNPTVNKRLE+M+V+ IP+E+ +KQF+VV
Sbjct: 121 FCWGVERAVQIAYEARKQFPEERLWITNEIIHNPTVNKRLEDMDVKIIPVEDSKKQFDVV 180

Query: 181 DKGDVVILPAFGAAVDEMLTLSEKKVQIVDTTCPWVSKVWNVVDKHKKGDYTSIIHGKYA 240
           +K DVVILPAFGA VDEM  L++KKVQIVDTTCPWV+KVWN V+KHKKG+YTS+IHGKY 
Sbjct: 181 EKDDVVILPAFGAGVDEMYVLNDKKVQIVDTTCPWVTKVWNTVEKHKKGEYTSVIHGKYN 240

Query: 241 HEETVATASFAGTYIIVKNMAEAIYVCDYILGGELDGSSSTKGEFMKKFKNAVSKGFDPD 300
           HEET+ATASFAG YIIVKNM EA YVCDYILGG+ DGSSSTK EFM+KFK A+SKGFDPD
Sbjct: 241 HEETIATASFAGKYIIVKNMKEANYVCDYILGGQYDGSSSTKEEFMEKFKYAISKGFDPD 300

Query: 301 VDLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDATQERQDAM 360
            DL+KVGIANQTTMLKGETEEIG+L+E TMMRK+GVENV+ HF+SFNTICDATQERQDA+
Sbjct: 301 NDLVKVGIANQTTMLKGETEEIGRLLETTMMRKYGVENVSGHFISFNTICDATQERQDAI 360

Query: 361 YKLVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHGEL 420
           Y+LVE K+DLMLVVGGWNSSNTSHLQEI+E RGIPSYWIDSE+RIGPGN+IAYKL++GEL
Sbjct: 361 YELVEEKIDLMLVVGGWNSSNTSHLQEISEARGIPSYWIDSEKRIGPGNKIAYKLHYGEL 420

Query: 421 VEKENWLPKGHITIGVTSGASTPDKVVEDALIKVFDIKREEALQFA 464
           VEKEN+LPKG ITIGVTSGASTPDKVVEDAL+KVFDIKREE LQ A
Sbjct: 421 VEKENFLPKGPITIGVTSGASTPDKVVEDALVKVFDIKREELLQLA 466

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q6AVG61.3e-21282.304-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic OS=Oryza sativ... [more]
Q94B354.9e-21277.044-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic OS=Arabidopsis... [more]
B1WTZ26.3e-15163.554-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Crocosphaera subtropica (s... [more]
Q31S642.0e-14962.944-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Synechococcus elongatus (s... [more]
Q5N2492.0e-14962.944-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Synechococcus sp. (strain ... [more]
Match NameE-valueIdentityDescription
A0A0A0K3D82.6e-26497.86Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G075680 PE=3 SV=1[more]
A0A5A7SJM13.7e-26397.844-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Cucumis melo var. makuwa O... [more]
A0A1S3BZP53.7e-26397.844-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like OS=Cucumi... [more]
A0A6J1I4K45.2e-24991.674-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic OS=Cucurbita m... [more]
K7NBR08.9e-24990.004-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Siraitia grosvenorii OX=19... [more]
Match NameE-valueIdentityDescription
XP_004137008.20.097.864-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic [Cucumis sativ... [more]
KAE8645891.10.097.86hypothetical protein Csa_017704 [Cucumis sativus][more]
XP_008454877.10.097.84PREDICTED: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-lik... [more]
XP_038888882.10.095.034-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic [Benincasa his... [more]
XP_022972347.11.64e-31591.674-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic [Cucurbita max... [more]
Match NameE-valueIdentityDescription
AT4G34350.13.5e-21377.044-hydroxy-3-methylbut-2-enyl diphosphate reductase [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR0034514-hydroxy-3-methylbut-2-enyl diphosphate reductaseTIGRFAMTIGR00216TIGR00216coord: 110..448
e-value: 1.6E-90
score: 301.1
IPR0034514-hydroxy-3-methylbut-2-enyl diphosphate reductasePFAMPF02401LYTBcoord: 110..448
e-value: 6.0E-83
score: 278.2
IPR0034514-hydroxy-3-methylbut-2-enyl diphosphate reductaseHAMAPMF_00191IspHcoord: 108..455
score: 28.334204
IPR0034514-hydroxy-3-methylbut-2-enyl diphosphate reductaseCDDcd13944lytB_ispHcoord: 110..448
e-value: 4.63264E-105
score: 311.652
NoneNo IPR availableGENE3D3.40.50.11270coord: 119..209
e-value: 1.4E-22
score: 81.6
NoneNo IPR availableGENE3D3.40.1010.20coord: 210..343
e-value: 9.5E-18
score: 66.1
NoneNo IPR availableGENE3D3.40.1010.20coord: 344..462
e-value: 8.1E-36
score: 124.2
NoneNo IPR availablePANTHERPTHR316194-HYDROXY-3-METHYLBUT-2-ENYL DIPHOSPHATE REDUCTASE, CHLOROPLASTICcoord: 1..461

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy1G000040.1Chy1G000040.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0050992 dimethylallyl diphosphate biosynthetic process
biological_process GO:0019288 isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway
molecular_function GO:0051745 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity
molecular_function GO:0051539 4 iron, 4 sulfur cluster binding
molecular_function GO:0046872 metal ion binding