Chy12G221190 (gene) Cucumber (hystrix) v1

Overview
NameChy12G221190
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionCaM_binding domain-containing protein
LocationchrH12: 18970588 .. 18972492 (-)
RNA-Seq ExpressionChy12G221190
SyntenyChy12G221190
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCAACGAAAATAGTGATATACCACTTGCTATGGAGGAGGTTAGTGAGGCTGAAGTTAGCCAAGAGGAGAGCTTTGATATTCCTGTCATTGCAGTTGCCAATACAAGTGAGCCTGAAGATATCACCGAGGAAATCATTGATATAATTGACATCATTGATATCCCAGCCACTATAGAGGTAAATGAGCCCGACATCATTGATATCCCAGCCACTATAGAGGTAAGTGAGCCCGAAAGTTGCAAGGTAGAAGTTATCGTGGACATTAATAGTAATACCCCAAAGATCAGACCAAGGGTTCTCTCTCGCTATCTTCGACCCCACACAGGCTCCTGCCACGATTTTTGCAAATATGGTTCAAAACATGCTCTTGAAGGGAAGCCTGCAAGTCCAGTCTCGAGAAAAGCTAAGTTAGTTGAAGGCAATGGCCAGGATTTGAGGAGGATTGTAGTTTCTTTGGCGAAACAAAACAAAGAATCTAACAGTCGGAAGTCCTCCCCGGAGTATAATCCCAACAATGTTACCGACTTGAAGGAAGATGTAATATCTTCCCCTGAGATTATTACTCCTTCTCCAAAGAGGCTTTTACCTTCCACCAAGGAAGTACAGGCTGCAGCAGTGCATTATAGTCGAACGAAACTTAATTTGTCTTTATCAAAGGTGTCTTCTTTTGCAGAACAAGGCAGTTCACGAACCAAAAGAAATAAAGAAATTCGAAAAGGAAAGAAAAAAGAAGGAGATGGAAGTTTAAGCAGTTCAAATAGTACAAGTAGAAGCCTAGAGATGAATGTCTCCGCAGAGGAGGACATACCAGCCTTGGTGCCAGAAGTAGGTTCTAGGACTCCAAGAACTCGTGTCAAGCGAGTTGCAATTGCAGATAAGAAAAGCATTGGGAGGAATGGCTTGAAAAGCCAAACCCATCCTATAAAATGTAAACCCGATCCATCTAACAATGAGGATGTGGAGGAGAAAACTTTATACATGATTGAACCGTCTACCAAGAATGAAACAGAGGAGATGTCTCAGAATAGTGTTCATACAACCGAGTCATCTCAACCACAATCTTCATCAACAATAGACAACAACTTGAAGCATGAACAAGAAGCAGCTGCGAACTCCATAGTGCCACCAATGTCAGTGAAAAAGAACGCCGTGAAACGTGCTAGAAATGGTCCCAGTTCTAAGATTTTATCTACATCTCCAACAGCATCCAAGGTATTCAAAGGTATTAGACCTAAGAGATTTGGTATGGTTCAAAGGTCCGAGACTCGATCAGCTCCTTCATCACCATTGTCATCTAGGTTCCAATCTGAACCCATCCATGTTGAGCACCGTGGCTCTACCTCAGGAAATGATGTGAAAAAAAGTGAGAATTCAAAGGTGGACCACAGGCTTAAGACCATTAGGATGACTCTAACCGATTCAGAAAATGGAGATTGCCAGTCTAGAAAGCTTAAGTTTAGGAAGGGCAAGACGGTTGAACTTCAACCTGAAACCAGTTCGCCAAGGAGGCTCAAATTTCGGCGTGTACGTTTACTCGGTGAGACTCAAAGTCCTAAAGGTGATTCAAGGAAGAGAAACATCAAGGGTAAAGAGGGGAACCAAAATGGGAAGGAAGGTGAGAATAGTTCTTTAAGACAGCAAGATAAGGATTTAAAGAAAAAGAGAAGCTTTAGGGATGGTAAATTGATTTCTAGTAGATTCAAATCTGAGAGAGTTGTCCTTAGGCATCAGGATTCAAAGGGAAAGAAAGAAATCCTAAATCTGTTTAATAATGTCATTGAAGAGACAGCAAGCAAGCTTGCAAAAACCAAAAAAAGTAAGGTGAAGGCTTTGGTTGGTGCCTTTGAAACTGTAATATCTCTTCAAGATACCAAACCTGCTGCAACAACAAGTGTTGCATGA

mRNA sequence

ATGGCCAACGAAAATAGTGATATACCACTTGCTATGGAGGAGGTTAGTGAGGCTGAAGTTAGCCAAGAGGAGAGCTTTGATATTCCTGTCATTGCAGTTGCCAATACAAGTGAGCCTGAAGATATCACCGAGGAAATCATTGATATAATTGACATCATTGATATCCCAGCCACTATAGAGGTAAATGAGCCCGACATCATTGATATCCCAGCCACTATAGAGGTAAGTGAGCCCGAAAGTTGCAAGGTAGAAGTTATCGTGGACATTAATAGTAATACCCCAAAGATCAGACCAAGGGTTCTCTCTCGCTATCTTCGACCCCACACAGGCTCCTGCCACGATTTTTGCAAATATGGTTCAAAACATGCTCTTGAAGGGAAGCCTGCAAGTCCAGTCTCGAGAAAAGCTAAGTTAGTTGAAGGCAATGGCCAGGATTTGAGGAGGATTGTAGTTTCTTTGGCGAAACAAAACAAAGAATCTAACAGTCGGAAGTCCTCCCCGGAGTATAATCCCAACAATGTTACCGACTTGAAGGAAGATGTAATATCTTCCCCTGAGATTATTACTCCTTCTCCAAAGAGGCTTTTACCTTCCACCAAGGAAGTACAGGCTGCAGCAGTGCATTATAGTCGAACGAAACTTAATTTGTCTTTATCAAAGGTGTCTTCTTTTGCAGAACAAGGCAGTTCACGAACCAAAAGAAATAAAGAAATTCGAAAAGGAAAGAAAAAAGAAGGAGATGGAAGTTTAAGCAGTTCAAATAGTACAAGTAGAAGCCTAGAGATGAATGTCTCCGCAGAGGAGGACATACCAGCCTTGGTGCCAGAAGTAGGTTCTAGGACTCCAAGAACTCGTGTCAAGCGAGTTGCAATTGCAGATAAGAAAAGCATTGGGAGGAATGGCTTGAAAAGCCAAACCCATCCTATAAAATGTAAACCCGATCCATCTAACAATGAGGATGTGGAGGAGAAAACTTTATACATGATTGAACCGTCTACCAAGAATGAAACAGAGGAGATGTCTCAGAATAGTGTTCATACAACCGAGTCATCTCAACCACAATCTTCATCAACAATAGACAACAACTTGAAGCATGAACAAGAAGCAGCTGCGAACTCCATAGTGCCACCAATGTCAGTGAAAAAGAACGCCGTGAAACGTGCTAGAAATGGTCCCAGTTCTAAGATTTTATCTACATCTCCAACAGCATCCAAGGTATTCAAAGGTATTAGACCTAAGAGATTTGGTATGGTTCAAAGGTCCGAGACTCGATCAGCTCCTTCATCACCATTGTCATCTAGGTTCCAATCTGAACCCATCCATGTTGAGCACCGTGGCTCTACCTCAGGAAATGATGTGAAAAAAAGTGAGAATTCAAAGGTGGACCACAGGCTTAAGACCATTAGGATGACTCTAACCGATTCAGAAAATGGAGATTGCCAGTCTAGAAAGCTTAAGTTTAGGAAGGGCAAGACGGTTGAACTTCAACCTGAAACCAGTTCGCCAAGGAGGCTCAAATTTCGGCGTGTACGTTTACTCGGTGAGACTCAAAGTCCTAAAGGTGATTCAAGGAAGAGAAACATCAAGGGTAAAGAGGGGAACCAAAATGGGAAGGAAGGTGAGAATAGTTCTTTAAGACAGCAAGATAAGGATTTAAAGAAAAAGAGAAGCTTTAGGGATGGTAAATTGATTTCTAGTAGATTCAAATCTGAGAGAGTTGTCCTTAGGCATCAGGATTCAAAGGGAAAGAAAGAAATCCTAAATCTGTTTAATAATGTCATTGAAGAGACAGCAAGCAAGCTTGCAAAAACCAAAAAAAGTAAGGTGAAGGCTTTGGTTGGTGCCTTTGAAACTGTAATATCTCTTCAAGATACCAAACCTGCTGCAACAACAAGTGTTGCATGA

Coding sequence (CDS)

ATGGCCAACGAAAATAGTGATATACCACTTGCTATGGAGGAGGTTAGTGAGGCTGAAGTTAGCCAAGAGGAGAGCTTTGATATTCCTGTCATTGCAGTTGCCAATACAAGTGAGCCTGAAGATATCACCGAGGAAATCATTGATATAATTGACATCATTGATATCCCAGCCACTATAGAGGTAAATGAGCCCGACATCATTGATATCCCAGCCACTATAGAGGTAAGTGAGCCCGAAAGTTGCAAGGTAGAAGTTATCGTGGACATTAATAGTAATACCCCAAAGATCAGACCAAGGGTTCTCTCTCGCTATCTTCGACCCCACACAGGCTCCTGCCACGATTTTTGCAAATATGGTTCAAAACATGCTCTTGAAGGGAAGCCTGCAAGTCCAGTCTCGAGAAAAGCTAAGTTAGTTGAAGGCAATGGCCAGGATTTGAGGAGGATTGTAGTTTCTTTGGCGAAACAAAACAAAGAATCTAACAGTCGGAAGTCCTCCCCGGAGTATAATCCCAACAATGTTACCGACTTGAAGGAAGATGTAATATCTTCCCCTGAGATTATTACTCCTTCTCCAAAGAGGCTTTTACCTTCCACCAAGGAAGTACAGGCTGCAGCAGTGCATTATAGTCGAACGAAACTTAATTTGTCTTTATCAAAGGTGTCTTCTTTTGCAGAACAAGGCAGTTCACGAACCAAAAGAAATAAAGAAATTCGAAAAGGAAAGAAAAAAGAAGGAGATGGAAGTTTAAGCAGTTCAAATAGTACAAGTAGAAGCCTAGAGATGAATGTCTCCGCAGAGGAGGACATACCAGCCTTGGTGCCAGAAGTAGGTTCTAGGACTCCAAGAACTCGTGTCAAGCGAGTTGCAATTGCAGATAAGAAAAGCATTGGGAGGAATGGCTTGAAAAGCCAAACCCATCCTATAAAATGTAAACCCGATCCATCTAACAATGAGGATGTGGAGGAGAAAACTTTATACATGATTGAACCGTCTACCAAGAATGAAACAGAGGAGATGTCTCAGAATAGTGTTCATACAACCGAGTCATCTCAACCACAATCTTCATCAACAATAGACAACAACTTGAAGCATGAACAAGAAGCAGCTGCGAACTCCATAGTGCCACCAATGTCAGTGAAAAAGAACGCCGTGAAACGTGCTAGAAATGGTCCCAGTTCTAAGATTTTATCTACATCTCCAACAGCATCCAAGGTATTCAAAGGTATTAGACCTAAGAGATTTGGTATGGTTCAAAGGTCCGAGACTCGATCAGCTCCTTCATCACCATTGTCATCTAGGTTCCAATCTGAACCCATCCATGTTGAGCACCGTGGCTCTACCTCAGGAAATGATGTGAAAAAAAGTGAGAATTCAAAGGTGGACCACAGGCTTAAGACCATTAGGATGACTCTAACCGATTCAGAAAATGGAGATTGCCAGTCTAGAAAGCTTAAGTTTAGGAAGGGCAAGACGGTTGAACTTCAACCTGAAACCAGTTCGCCAAGGAGGCTCAAATTTCGGCGTGTACGTTTACTCGGTGAGACTCAAAGTCCTAAAGGTGATTCAAGGAAGAGAAACATCAAGGGTAAAGAGGGGAACCAAAATGGGAAGGAAGGTGAGAATAGTTCTTTAAGACAGCAAGATAAGGATTTAAAGAAAAAGAGAAGCTTTAGGGATGGTAAATTGATTTCTAGTAGATTCAAATCTGAGAGAGTTGTCCTTAGGCATCAGGATTCAAAGGGAAAGAAAGAAATCCTAAATCTGTTTAATAATGTCATTGAAGAGACAGCAAGCAAGCTTGCAAAAACCAAAAAAAGTAAGGTGAAGGCTTTGGTTGGTGCCTTTGAAACTGTAATATCTCTTCAAGATACCAAACCTGCTGCAACAACAAGTGTTGCATGA

Protein sequence

MANENSDIPLAMEEVSEAEVSQEESFDIPVIAVANTSEPEDITEEIIDIIDIIDIPATIEVNEPDIIDIPATIEVSEPESCKVEVIVDINSNTPKIRPRVLSRYLRPHTGSCHDFCKYGSKHALEGKPASPVSRKAKLVEGNGQDLRRIVVSLAKQNKESNSRKSSPEYNPNNVTDLKEDVISSPEIITPSPKRLLPSTKEVQAAAVHYSRTKLNLSLSKVSSFAEQGSSRTKRNKEIRKGKKKEGDGSLSSSNSTSRSLEMNVSAEEDIPALVPEVGSRTPRTRVKRVAIADKKSIGRNGLKSQTHPIKCKPDPSNNEDVEEKTLYMIEPSTKNETEEMSQNSVHTTESSQPQSSSTIDNNLKHEQEAAANSIVPPMSVKKNAVKRARNGPSSKILSTSPTASKVFKGIRPKRFGMVQRSETRSAPSSPLSSRFQSEPIHVEHRGSTSGNDVKKSENSKVDHRLKTIRMTLTDSENGDCQSRKLKFRKGKTVELQPETSSPRRLKFRRVRLLGETQSPKGDSRKRNIKGKEGNQNGKEGENSSLRQQDKDLKKKRSFRDGKLISSRFKSERVVLRHQDSKGKKEILNLFNNVIEETASKLAKTKKSKVKALVGAFETVISLQDTKPAATTSVA*
Homology
BLAST of Chy12G221190 vs. ExPASy TrEMBL
Match: A0A0A0LST4 (CaM_binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G038960 PE=4 SV=1)

HSP 1 Score: 1105.5 bits (2858), Expect = 0.0e+00
Identity = 603/634 (95.11%), Postives = 616/634 (97.16%), Query Frame = 0

Query: 1   MANENSDIPLAMEEVSEAEVSQEESFDIPVIAVANTSEPEDITEEIIDIIDIIDIPATIE 60
           MANENSDIPLAMEEVSEAEVSQEESFDIPVIAVAN SEPEDITEEIIDIIDIIDIPATIE
Sbjct: 1   MANENSDIPLAMEEVSEAEVSQEESFDIPVIAVANISEPEDITEEIIDIIDIIDIPATIE 60

Query: 61  VNEPDIIDIPATIEVSEPESCKVEVIVDINSNTPKIRPRVLSRYLRPHTGSCHDFCKYGS 120
           VNEPDI+DIPATIEVSEPESCKVEVI+DINSN PKIRPRVLSRYL P+TGSCHDFCKYGS
Sbjct: 61  VNEPDIVDIPATIEVSEPESCKVEVIMDINSNIPKIRPRVLSRYLLPYTGSCHDFCKYGS 120

Query: 121 KHALEGKPASPVSRKAKLVEGNGQDLRRIVVSLAKQNKESNSRKSSPEYNPNNVTDLKED 180
           KH LEGKPASP+SRKAKLV GNGQDLRR VVSLAKQNKESNSRKSS EYNP+NVTDLKED
Sbjct: 121 KHDLEGKPASPISRKAKLVGGNGQDLRRTVVSLAKQNKESNSRKSSLEYNPSNVTDLKED 180

Query: 181 VISSPEIITPSPKRLLPSTKEVQAAAVHYSRTKLNLSLSKVSSFAEQGSSRTKRNKEIRK 240
           +ISSPEI+TPSPKRLLPSTKEVQAAAVHYSRTKLNLSLSKVSSFA QG SRTKRNKEIRK
Sbjct: 181 IISSPEIVTPSPKRLLPSTKEVQAAAVHYSRTKLNLSLSKVSSFAGQGGSRTKRNKEIRK 240

Query: 241 GKKKEGDGSLSSSNSTSRSLEMNVSAEEDIPALVPEVGSRTPRTRVKRVAIADKKSIGRN 300
           GKKKEGDGSLSSSNSTSRSLEMNVSAEEDI ALVPEVGSRTPRTRVKRVAIADKK+IGRN
Sbjct: 241 GKKKEGDGSLSSSNSTSRSLEMNVSAEEDITALVPEVGSRTPRTRVKRVAIADKKNIGRN 300

Query: 301 GLKSQTHPIKCKPDPSNNEDVEEKTLYMIEPSTKNETEEMSQNSVHTTESSQPQSSSTID 360
           GLKSQTHPIKCKPDPSNNEDVEEKTLYMIEPSTK+ETEE+SQNSVHTTESSQPQSSST D
Sbjct: 301 GLKSQTHPIKCKPDPSNNEDVEEKTLYMIEPSTKDETEEISQNSVHTTESSQPQSSSTTD 360

Query: 361 NNLKHEQEAAANSIVPPMSVKKNAVKRARNGPSSKILSTSPTASKVFKGIRPKRFGMVQR 420
           NNLKHEQEAAANSIVPPMSVKKN VKRARNG S+KIL TSPTASKVFKGIRPKRFGMVQR
Sbjct: 361 NNLKHEQEAAANSIVPPMSVKKNVVKRARNGTSAKILCTSPTASKVFKGIRPKRFGMVQR 420

Query: 421 SETRSAPSSPLSSRFQSEPIHVEHRGSTSGNDVKKSENSKVDHRLKTIRMTLTDSENGDC 480
           SETRSAPSSPLSSRFQSEPIHVEHRGSTSGNDVKKSENSKVDHRLKT  MTLTDSENGDC
Sbjct: 421 SETRSAPSSPLSSRFQSEPIHVEHRGSTSGNDVKKSENSKVDHRLKTKGMTLTDSENGDC 480

Query: 481 QSRKLKFRKGKTVELQPETSSPRRLKFRRVRLLGETQSPKGDSRKRNIKGKEGNQNGKEG 540
           QSRKLKFRKGKTVELQPETSSPRRLKFR VRLLGETQSPKGDSRKRNI GK+GNQNGKEG
Sbjct: 481 QSRKLKFRKGKTVELQPETSSPRRLKFRHVRLLGETQSPKGDSRKRNIMGKDGNQNGKEG 540

Query: 541 ENSSLRQQDKDLKKKRSFRDGKLISSRFKSERVVLRHQDSKGKKEILNLFNNVIEETASK 600
           ENSSLRQQDKDLKKKRSFRDGKLISSRFKSERVVLRHQDSKGKKEILNLFNNVIEETASK
Sbjct: 541 ENSSLRQQDKDLKKKRSFRDGKLISSRFKSERVVLRHQDSKGKKEILNLFNNVIEETASK 600

Query: 601 LAKTKKSKVKALVGAFETVISLQDTKPAATTSVA 635
           LAKT+KSKVKALVGAFETVISLQDTKPAATTSVA
Sbjct: 601 LAKTRKSKVKALVGAFETVISLQDTKPAATTSVA 634

BLAST of Chy12G221190 vs. ExPASy TrEMBL
Match: A0A1S3BFX3 (uncharacterized protein LOC103489380 OS=Cucumis melo OX=3656 GN=LOC103489380 PE=4 SV=1)

HSP 1 Score: 1019.6 bits (2635), Expect = 5.6e-294
Identity = 567/637 (89.01%), Postives = 588/637 (92.31%), Query Frame = 0

Query: 1   MANENSDIPLAMEEVSEAEVSQEESFDIPVIAVANTSEPEDITEEIIDIIDIIDIPATIE 60
           MA+ENSDIPLAMEEVSEAEVSQEESFDIPV+AVAN SEPEDITEE IDIIDIIDIPATIE
Sbjct: 1   MADENSDIPLAMEEVSEAEVSQEESFDIPVVAVANISEPEDITEESIDIIDIIDIPATIE 60

Query: 61  VNEPDIIDIPATIEVSEPESCKVEVIVDINSNTPKIRPRVLSRYLRPHTGSCHDFCKYGS 120
           VN              EPESCKVEVIVD +SNTPKIRP+VLSRYLRPHTGSCHDFCKYGS
Sbjct: 61  VN--------------EPESCKVEVIVDTDSNTPKIRPKVLSRYLRPHTGSCHDFCKYGS 120

Query: 121 KHALEGKPASPVSRKAKLVEGNGQDLRRIVVSLAKQNKESNSRKSSPEYNPNNVTDLKED 180
           +HALEGKPASPVSRKAKLV GN QDLRR +VSLAKQNKESNS KSS EYNP N+TDLKED
Sbjct: 121 EHALEGKPASPVSRKAKLVGGNSQDLRRTIVSLAKQNKESNSPKSSQEYNPINITDLKED 180

Query: 181 VISSPEIITPSPKRLLPSTKEVQAAAVHYSRTKLNLSLSKVSSFAEQGSSRTKRNKEIRK 240
           +ISSPEI+TP PKRLLPSTKEVQAAAVHYSRTKLNLS SKVSSFA QGSSRTKRNKEIRK
Sbjct: 181 IISSPEIVTP-PKRLLPSTKEVQAAAVHYSRTKLNLSSSKVSSFAGQGSSRTKRNKEIRK 240

Query: 241 GKKKEGDGSLSSSNSTSRSLEMNVSAEEDIPALVPEVGSRTPRTRVKRVAIADKKSIGRN 300
           GKKK+GDGSLSSSNSTSRSLEMN+SAEEDI ALVPEVGSRTP+TRVKRVAIADKKSIGRN
Sbjct: 241 GKKKDGDGSLSSSNSTSRSLEMNISAEEDITALVPEVGSRTPKTRVKRVAIADKKSIGRN 300

Query: 301 GLKSQTHPIKCKPDPSNNEDVEEKTLYMIEPSTKNETEEMSQNSVHTTESSQPQSSSTID 360
           GLKSQ H IKCKPDPSNNEDVEEKTLYMIEPS+KNETE MSQ+S+HTTESSQPQSSST D
Sbjct: 301 GLKSQIHHIKCKPDPSNNEDVEEKTLYMIEPSSKNETEGMSQSSLHTTESSQPQSSSTTD 360

Query: 361 NNLKHEQEAAANSIVPPMSVKKNAVKRARNGPSSKILSTSPTASKVFKGIRPKRFGMVQR 420
           NNLKHEQEA ANSIVPPMS KKN VKRARNG S KILSTSPT   VFKGIRPKRFGMVQR
Sbjct: 361 NNLKHEQEAVANSIVPPMSEKKNVVKRARNGTSPKILSTSPT---VFKGIRPKRFGMVQR 420

Query: 421 SETRSAPSSPLSSRFQSEPIHVEHRGSTSGNDVKKSENSKVDHRLKTIRMTLTDSENGDC 480
           SETRSAPSSPLSSR QSEPIHVEHRGSTSGN VKK ENSKVDHRLKT RMTLTDSENGDC
Sbjct: 421 SETRSAPSSPLSSRSQSEPIHVEHRGSTSGNGVKKIENSKVDHRLKTRRMTLTDSENGDC 480

Query: 481 QSRKLKFRKGKTVELQPETSSPRRLKFRRVRLLGETQSPKGDSRKRNIKGKEGNQNG--- 540
           QSRKLKFRKG+ VELQPETS+PRRLKFRRVRLLGE +SPKGDSRKRNIKGKEGNQNG   
Sbjct: 481 QSRKLKFRKGRMVELQPETSTPRRLKFRRVRLLGEAESPKGDSRKRNIKGKEGNQNGKEV 540

Query: 541 KEGENSSLRQQDKDLKKKRSFRDGKLISSRFKSERVVLRHQDSKGKKEILNLFNNVIEET 600
           KEGENSSLRQQDKDLKKKRSFRDGKL+SSRFKSERVVLRHQDSKGKKE+LNLFNNVIEET
Sbjct: 541 KEGENSSLRQQDKDLKKKRSFRDGKLVSSRFKSERVVLRHQDSKGKKEVLNLFNNVIEET 600

Query: 601 ASKLAKTKKSKVKALVGAFETVISLQDTKPAATTSVA 635
           ASKLAKT+KSKVKALVGAFETVISLQDTKP ATTSVA
Sbjct: 601 ASKLAKTRKSKVKALVGAFETVISLQDTKPTATTSVA 619

BLAST of Chy12G221190 vs. ExPASy TrEMBL
Match: A0A5D3BIK3 (CaM_binding domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G001130 PE=4 SV=1)

HSP 1 Score: 1019.6 bits (2635), Expect = 5.6e-294
Identity = 567/637 (89.01%), Postives = 588/637 (92.31%), Query Frame = 0

Query: 1   MANENSDIPLAMEEVSEAEVSQEESFDIPVIAVANTSEPEDITEEIIDIIDIIDIPATIE 60
           MA+ENSDIPLAMEEVSEAEVSQEESFDIPV+AVAN SEPEDITEE IDIIDIIDIPATIE
Sbjct: 1   MADENSDIPLAMEEVSEAEVSQEESFDIPVVAVANISEPEDITEESIDIIDIIDIPATIE 60

Query: 61  VNEPDIIDIPATIEVSEPESCKVEVIVDINSNTPKIRPRVLSRYLRPHTGSCHDFCKYGS 120
           VN              EPESCKVEVIVD +SNTPKIRP+VLSRYLRPHTGSCHDFCKYGS
Sbjct: 61  VN--------------EPESCKVEVIVDTDSNTPKIRPKVLSRYLRPHTGSCHDFCKYGS 120

Query: 121 KHALEGKPASPVSRKAKLVEGNGQDLRRIVVSLAKQNKESNSRKSSPEYNPNNVTDLKED 180
           +HALEGKPASPVSRKAKLV GN QDLRR +VSLAKQNKESNS KSS EYNP N+TDLKED
Sbjct: 121 EHALEGKPASPVSRKAKLVGGNSQDLRRTIVSLAKQNKESNSPKSSQEYNPINITDLKED 180

Query: 181 VISSPEIITPSPKRLLPSTKEVQAAAVHYSRTKLNLSLSKVSSFAEQGSSRTKRNKEIRK 240
           +ISSPEI+TP PKRLLPSTKEVQAAAVHYSRTKLNLS SKVSSFA QGSSRTKRNKEIRK
Sbjct: 181 IISSPEIVTP-PKRLLPSTKEVQAAAVHYSRTKLNLSSSKVSSFAGQGSSRTKRNKEIRK 240

Query: 241 GKKKEGDGSLSSSNSTSRSLEMNVSAEEDIPALVPEVGSRTPRTRVKRVAIADKKSIGRN 300
           GKKK+GDGSLSSSNSTSRSLEMN+SAEEDI ALVPEVGSRTP+TRVKRVAIADKKSIGRN
Sbjct: 241 GKKKDGDGSLSSSNSTSRSLEMNISAEEDITALVPEVGSRTPKTRVKRVAIADKKSIGRN 300

Query: 301 GLKSQTHPIKCKPDPSNNEDVEEKTLYMIEPSTKNETEEMSQNSVHTTESSQPQSSSTID 360
           GLKSQ H IKCKPDPSNNEDVEEKTLYMIEPS+KNETE MSQ+S+HTTESSQPQSSST D
Sbjct: 301 GLKSQIHHIKCKPDPSNNEDVEEKTLYMIEPSSKNETEGMSQSSLHTTESSQPQSSSTTD 360

Query: 361 NNLKHEQEAAANSIVPPMSVKKNAVKRARNGPSSKILSTSPTASKVFKGIRPKRFGMVQR 420
           NNLKHEQEA ANSIVPPMS KKN VKRARNG S KILSTSPT   VFKGIRPKRFGMVQR
Sbjct: 361 NNLKHEQEAVANSIVPPMSEKKNVVKRARNGTSPKILSTSPT---VFKGIRPKRFGMVQR 420

Query: 421 SETRSAPSSPLSSRFQSEPIHVEHRGSTSGNDVKKSENSKVDHRLKTIRMTLTDSENGDC 480
           SETRSAPSSPLSSR QSEPIHVEHRGSTSGN VKK ENSKVDHRLKT RMTLTDSENGDC
Sbjct: 421 SETRSAPSSPLSSRSQSEPIHVEHRGSTSGNGVKKIENSKVDHRLKTRRMTLTDSENGDC 480

Query: 481 QSRKLKFRKGKTVELQPETSSPRRLKFRRVRLLGETQSPKGDSRKRNIKGKEGNQNG--- 540
           QSRKLKFRKG+ VELQPETS+PRRLKFRRVRLLGE +SPKGDSRKRNIKGKEGNQNG   
Sbjct: 481 QSRKLKFRKGRMVELQPETSTPRRLKFRRVRLLGEAESPKGDSRKRNIKGKEGNQNGKEV 540

Query: 541 KEGENSSLRQQDKDLKKKRSFRDGKLISSRFKSERVVLRHQDSKGKKEILNLFNNVIEET 600
           KEGENSSLRQQDKDLKKKRSFRDGKL+SSRFKSERVVLRHQDSKGKKE+LNLFNNVIEET
Sbjct: 541 KEGENSSLRQQDKDLKKKRSFRDGKLVSSRFKSERVVLRHQDSKGKKEVLNLFNNVIEET 600

Query: 601 ASKLAKTKKSKVKALVGAFETVISLQDTKPAATTSVA 635
           ASKLAKT+KSKVKALVGAFETVISLQDTKP ATTSVA
Sbjct: 601 ASKLAKTRKSKVKALVGAFETVISLQDTKPTATTSVA 619

BLAST of Chy12G221190 vs. ExPASy TrEMBL
Match: A0A6J1KGB0 (uncharacterized protein LOC111492963 OS=Cucurbita maxima OX=3661 GN=LOC111492963 PE=4 SV=1)

HSP 1 Score: 637.9 bits (1644), Expect = 4.6e-179
Identity = 402/639 (62.91%), Postives = 463/639 (72.46%), Query Frame = 0

Query: 1   MANENSDIPLAMEEVSEAEVSQEESFDIPVIAVANTSEPEDITEEIIDIIDIIDIPATIE 60
           MA EN+DIPL + EVS  E  Q ESFDIP     N  EP  I E   DI   ++  A  E
Sbjct: 1   MAGENTDIPLVV-EVSGDEGRQHESFDIPTNMEVN--EPNAIIEGSTDISLALEASAD-E 60

Query: 61  VNEPDIIDIPATIEVSEPESCKVEVIVDINSNTPKIRPRVLSRYLRPHTGSCHDFCKYGS 120
            ++ +  DIP T+EV+EPESC V VIV+I  NTPK R R+  RYL P TGSCHDFCKYG+
Sbjct: 61  GSQDEKFDIPTTMEVNEPESCNVGVIVNI--NTPKFR-RIFPRYLSPRTGSCHDFCKYGT 120

Query: 121 KHALEGKPASPVSRKAKLVEGNGQDLRRIVVSLAKQNKESNSRKSSPEYNPNNVTDLKED 180
           KH +E  PAS V RK K V  + +DLRRI V+LAK N ++ S K S +Y+  N+TDLKED
Sbjct: 121 KHPIERNPASAVLRKGKSVGRDVRDLRRIRVTLAKPNNDTVSPKFSKDYDGINITDLKED 180

Query: 181 VISSPEIITPSPKRLLPSTKEVQAAAVHYSRTKLNLSLSKVSSFAEQGSSRTKRNKEIRK 240
           V SSPEII PSPK+ LP  KEV+AAAV YSRTKLNLSLSK SS A Q +SRT RNKE+R+
Sbjct: 181 VTSSPEIINPSPKKRLPPIKEVEAAAVQYSRTKLNLSLSKASSSAGQCTSRTNRNKEVRR 240

Query: 241 GKKKEGDGSLSSS-NSTSRSLEMNVSAEEDIPALVPEVGSRTPRTRVKRVAIADKKSIGR 300
            KK++G GS SSS +STSR  E+ +S   D  ALVP   S TPR RVKRVAI DKK IGR
Sbjct: 241 SKKQDGYGSSSSSTDSTSRCQEIKISTVGDRKALVPHAVSWTPRNRVKRVAIVDKKIIGR 300

Query: 301 NGLKSQTHPIKCKPDPSNNEDVEEKTLYMIEPSTKNETEEMSQNSVHTTESSQPQSSSTI 360
           + LK+Q+   K KPDPSN+E VEEKTLYMIEPSTK ETE  +QNSVH TE S PQSSS  
Sbjct: 301 HSLKNQSRLKKIKPDPSNSEVVEEKTLYMIEPSTKKETEGPAQNSVHFTELSHPQSSSAT 360

Query: 361 DNNLKHEQEAAANSIVPPMSVKKNAVKRARNGPSSKILSTSPTASKVFKGIRPKRFGMVQ 420
           DN+ KH+QEA    I P + V++N  +R RNG SSK LSTSPT  K FKG+RPKRF M+Q
Sbjct: 361 DNSFKHKQEADGTIITPHLLVEEN-TRRTRNGTSSKSLSTSPTVFKGFKGLRPKRFDMIQ 420

Query: 421 RSETRSAPSSPLSSRFQSEPIHVEHRGSTSGNDVKKSENSKVDHRLKTIRMTLTDSENGD 480
            SETRSAPSSP SSR  SEP+H               E SKV+H++K  R TLTDSENGD
Sbjct: 421 MSETRSAPSSPSSSRCSSEPVH--------------GEKSKVEHKIKARRKTLTDSENGD 480

Query: 481 CQSRKLKFRKGKTVELQPETSSPRRLKFRRVRLLGETQSPKGDSRKRNIKGKEGNQNG-- 540
           CQSRKL FRKG+ VELQ E  +PRRL F+RVR L ETQSPK DSRKR I+ KE NQNG  
Sbjct: 481 CQSRKLHFRKGRMVELQTEPITPRRLIFKRVRSLSETQSPKSDSRKRIIQRKEANQNGDE 540

Query: 541 -KEGENSSLRQQDKDLKKKRSFR-----DGKLISSRFKSERVVLRHQDSKGKKEILNLFN 600
            KE ENSSLRQQD++ K+K+SFR     DGKL+SSR KSER+VL+HQDS  K EI  L N
Sbjct: 541 VKETENSSLRQQDQEFKRKKSFRRQETIDGKLVSSRLKSERIVLKHQDSNEKIEIQKLLN 600

Query: 601 NVIEETASKLAKTKKSKVKALVGAFETVISLQDTKPAAT 631
           NVIEETA+KLAKT+KSKVKALVGAFETVISLQD KPAAT
Sbjct: 601 NVIEETATKLAKTRKSKVKALVGAFETVISLQDAKPAAT 617

BLAST of Chy12G221190 vs. ExPASy TrEMBL
Match: A0A6J1KLU3 (uncharacterized protein LOC111495740 OS=Cucurbita maxima OX=3661 GN=LOC111495740 PE=4 SV=1)

HSP 1 Score: 635.2 bits (1637), Expect = 3.0e-178
Identity = 449/947 (47.41%), Postives = 514/947 (54.28%), Query Frame = 0

Query: 1   MANENSDIPLAMEEVSEAEVSQEESFDIPVIAVANTSEPEDITEEIIDIIDIIDIPATIE 60
           MA +N+DIPLAMEEVS  E +Q+ES DIPVI VA  S+PEDITEE ID    I + A  E
Sbjct: 1   MAKQNNDIPLAMEEVSGDEATQDESLDIPVIEVAEISKPEDITEESID----IPVIAVAE 60

Query: 61  VNEP--------DIIDIPATIEVSEPESCKVEVIVDINSNTPKIRPRVLSRYLRPHTGSC 120
            +EP        DIIDIPAT EV+EPESC VEVIV I  NTPK++P++LSRYL PHTGSC
Sbjct: 61  TSEPEDITEKGIDIIDIPATTEVNEPESCNVEVIVHI--NTPKMKPKILSRYLLPHTGSC 120

Query: 121 HDFCKYGSKHALEGKPASPVSRKAKLVEGNGQDLRRIVVSLAKQNKESNSRKSSPEYNPN 180
           HDFCKYG+K  LEGKPAS + RKAK + G G+DLRRI+V LAKQN  + + KSSP+YN  
Sbjct: 121 HDFCKYGAKQDLEGKPASSILRKAKSMGGGGRDLRRIMVLLAKQN--TITPKSSPDYNAI 180

Query: 181 NVTDLKEDVISSPEIITPSPKRLLPSTKEVQAAAVHYSRTKLNLSLSKVSSFAEQGSSRT 240
           N+TD+KED+IS PEI+TPSPKR LPS KEVQAA+VHY RTKLNLS S  SSFA + SSRT
Sbjct: 181 NITDVKEDIISFPEIVTPSPKRPLPSIKEVQAASVHYGRTKLNLSRSMASSFARRVSSRT 240

Query: 241 KRNKEIRKGKKKEGDGSLSS-SNSTSRSLEMNVSAEEDIPALVPEVGSRTPRTRVKRVAI 300
           KRNKE+RK KK++GDGS SS +NSTSR  E N+SAEED+  LVP V  R PR RVK V I
Sbjct: 241 KRNKELRKDKKQDGDGSSSSCTNSTSRCQERNISAEEDMKDLVPRVLFRVPRNRVKCVTI 300

Query: 301 ADKKSIGRNGLKSQTHPIKCKPDPSNNEDVEEKTLYMIEPSTKNETEEMSQNSVHTTES- 360
           ADKK IGR GLK   HP K KPDP NNEDVEEKTLYMIEPSTKNET+E++QNSV T ES 
Sbjct: 301 ADKKIIGRCGLKRPKHPRKYKPDPFNNEDVEEKTLYMIEPSTKNETQELAQNSVQTAESR 360

Query: 361 ------------------------------------------------------------ 420
                                                                       
Sbjct: 361 SQSSSATDNSLKHEQESDRSSVLPPLSVKKNMRHARNRTNPKLLSTKNETEALTQKCIQT 420

Query: 421 --SQPQSSSTIDNNLKHEQEAAANSIVPPMSVKKNA------------------------ 480
             S+PQSSS  D++LKHEQE+  + IVPP+SVKKN                         
Sbjct: 421 AESRPQSSSATDSSLKHEQESDGSPIVPPLSVKKNVRRARNRTSPKILSTKNETEELTQN 480

Query: 481 ------------------------------------------------------------ 540
                                                                       
Sbjct: 481 SVQTAESRLQYSSATDNSLKHEQESDGAPIVSPFSVKKNVRRARSRTSPKTLSTKNETEK 540

Query: 541 ------------------------------------------------------------ 600
                                                                       
Sbjct: 541 LVQNSVQTAKSRPQSSFATDNRLKHQIESDGAPIVSPWSVKKNVRRVRNRTSPKILSTKN 600

Query: 601 -----------------------------------------------VKRARN------- 630
                                                          V+RARN       
Sbjct: 601 ETGELAKNSVQTAVSRPQSSFATDNSLKHQQESDGAPIVPPLSVKKKVRRARNRTTPKSL 660

BLAST of Chy12G221190 vs. NCBI nr
Match: XP_011650019.1 (uncharacterized protein LOC105434693 [Cucumis sativus] >XP_011650022.1 uncharacterized protein LOC105434693 [Cucumis sativus] >KGN64029.1 hypothetical protein Csa_013389 [Cucumis sativus])

HSP 1 Score: 1109 bits (2868), Expect = 0.0
Identity = 603/634 (95.11%), Postives = 616/634 (97.16%), Query Frame = 0

Query: 1   MANENSDIPLAMEEVSEAEVSQEESFDIPVIAVANTSEPEDITEEIIDIIDIIDIPATIE 60
           MANENSDIPLAMEEVSEAEVSQEESFDIPVIAVAN SEPEDITEEIIDIIDIIDIPATIE
Sbjct: 1   MANENSDIPLAMEEVSEAEVSQEESFDIPVIAVANISEPEDITEEIIDIIDIIDIPATIE 60

Query: 61  VNEPDIIDIPATIEVSEPESCKVEVIVDINSNTPKIRPRVLSRYLRPHTGSCHDFCKYGS 120
           VNEPDI+DIPATIEVSEPESCKVEVI+DINSN PKIRPRVLSRYL P+TGSCHDFCKYGS
Sbjct: 61  VNEPDIVDIPATIEVSEPESCKVEVIMDINSNIPKIRPRVLSRYLLPYTGSCHDFCKYGS 120

Query: 121 KHALEGKPASPVSRKAKLVEGNGQDLRRIVVSLAKQNKESNSRKSSPEYNPNNVTDLKED 180
           KH LEGKPASP+SRKAKLV GNGQDLRR VVSLAKQNKESNSRKSS EYNP+NVTDLKED
Sbjct: 121 KHDLEGKPASPISRKAKLVGGNGQDLRRTVVSLAKQNKESNSRKSSLEYNPSNVTDLKED 180

Query: 181 VISSPEIITPSPKRLLPSTKEVQAAAVHYSRTKLNLSLSKVSSFAEQGSSRTKRNKEIRK 240
           +ISSPEI+TPSPKRLLPSTKEVQAAAVHYSRTKLNLSLSKVSSFA QG SRTKRNKEIRK
Sbjct: 181 IISSPEIVTPSPKRLLPSTKEVQAAAVHYSRTKLNLSLSKVSSFAGQGGSRTKRNKEIRK 240

Query: 241 GKKKEGDGSLSSSNSTSRSLEMNVSAEEDIPALVPEVGSRTPRTRVKRVAIADKKSIGRN 300
           GKKKEGDGSLSSSNSTSRSLEMNVSAEEDI ALVPEVGSRTPRTRVKRVAIADKK+IGRN
Sbjct: 241 GKKKEGDGSLSSSNSTSRSLEMNVSAEEDITALVPEVGSRTPRTRVKRVAIADKKNIGRN 300

Query: 301 GLKSQTHPIKCKPDPSNNEDVEEKTLYMIEPSTKNETEEMSQNSVHTTESSQPQSSSTID 360
           GLKSQTHPIKCKPDPSNNEDVEEKTLYMIEPSTK+ETEE+SQNSVHTTESSQPQSSST D
Sbjct: 301 GLKSQTHPIKCKPDPSNNEDVEEKTLYMIEPSTKDETEEISQNSVHTTESSQPQSSSTTD 360

Query: 361 NNLKHEQEAAANSIVPPMSVKKNAVKRARNGPSSKILSTSPTASKVFKGIRPKRFGMVQR 420
           NNLKHEQEAAANSIVPPMSVKKN VKRARNG S+KIL TSPTASKVFKGIRPKRFGMVQR
Sbjct: 361 NNLKHEQEAAANSIVPPMSVKKNVVKRARNGTSAKILCTSPTASKVFKGIRPKRFGMVQR 420

Query: 421 SETRSAPSSPLSSRFQSEPIHVEHRGSTSGNDVKKSENSKVDHRLKTIRMTLTDSENGDC 480
           SETRSAPSSPLSSRFQSEPIHVEHRGSTSGNDVKKSENSKVDHRLKT  MTLTDSENGDC
Sbjct: 421 SETRSAPSSPLSSRFQSEPIHVEHRGSTSGNDVKKSENSKVDHRLKTKGMTLTDSENGDC 480

Query: 481 QSRKLKFRKGKTVELQPETSSPRRLKFRRVRLLGETQSPKGDSRKRNIKGKEGNQNGKEG 540
           QSRKLKFRKGKTVELQPETSSPRRLKFR VRLLGETQSPKGDSRKRNI GK+GNQNGKEG
Sbjct: 481 QSRKLKFRKGKTVELQPETSSPRRLKFRHVRLLGETQSPKGDSRKRNIMGKDGNQNGKEG 540

Query: 541 ENSSLRQQDKDLKKKRSFRDGKLISSRFKSERVVLRHQDSKGKKEILNLFNNVIEETASK 600
           ENSSLRQQDKDLKKKRSFRDGKLISSRFKSERVVLRHQDSKGKKEILNLFNNVIEETASK
Sbjct: 541 ENSSLRQQDKDLKKKRSFRDGKLISSRFKSERVVLRHQDSKGKKEILNLFNNVIEETASK 600

Query: 601 LAKTKKSKVKALVGAFETVISLQDTKPAATTSVA 634
           LAKT+KSKVKALVGAFETVISLQDTKPAATTSVA
Sbjct: 601 LAKTRKSKVKALVGAFETVISLQDTKPAATTSVA 634

BLAST of Chy12G221190 vs. NCBI nr
Match: XP_008446785.1 (PREDICTED: uncharacterized protein LOC103489380 [Cucumis melo] >XP_008446868.1 PREDICTED: uncharacterized protein LOC103489380 [Cucumis melo] >XP_008446947.1 PREDICTED: uncharacterized protein LOC103489380 [Cucumis melo] >XP_008447016.1 PREDICTED: uncharacterized protein LOC103489380 [Cucumis melo] >TYJ98916.1 CaM_binding domain-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 1023 bits (2646), Expect = 0.0
Identity = 567/637 (89.01%), Postives = 588/637 (92.31%), Query Frame = 0

Query: 1   MANENSDIPLAMEEVSEAEVSQEESFDIPVIAVANTSEPEDITEEIIDIIDIIDIPATIE 60
           MA+ENSDIPLAMEEVSEAEVSQEESFDIPV+AVAN SEPEDITEE IDIIDIIDIPATIE
Sbjct: 1   MADENSDIPLAMEEVSEAEVSQEESFDIPVVAVANISEPEDITEESIDIIDIIDIPATIE 60

Query: 61  VNEPDIIDIPATIEVSEPESCKVEVIVDINSNTPKIRPRVLSRYLRPHTGSCHDFCKYGS 120
           VNEP              ESCKVEVIVD +SNTPKIRP+VLSRYLRPHTGSCHDFCKYGS
Sbjct: 61  VNEP--------------ESCKVEVIVDTDSNTPKIRPKVLSRYLRPHTGSCHDFCKYGS 120

Query: 121 KHALEGKPASPVSRKAKLVEGNGQDLRRIVVSLAKQNKESNSRKSSPEYNPNNVTDLKED 180
           +HALEGKPASPVSRKAKLV GN QDLRR +VSLAKQNKESNS KSS EYNP N+TDLKED
Sbjct: 121 EHALEGKPASPVSRKAKLVGGNSQDLRRTIVSLAKQNKESNSPKSSQEYNPINITDLKED 180

Query: 181 VISSPEIITPSPKRLLPSTKEVQAAAVHYSRTKLNLSLSKVSSFAEQGSSRTKRNKEIRK 240
           +ISSPEI+TP PKRLLPSTKEVQAAAVHYSRTKLNLS SKVSSFA QGSSRTKRNKEIRK
Sbjct: 181 IISSPEIVTP-PKRLLPSTKEVQAAAVHYSRTKLNLSSSKVSSFAGQGSSRTKRNKEIRK 240

Query: 241 GKKKEGDGSLSSSNSTSRSLEMNVSAEEDIPALVPEVGSRTPRTRVKRVAIADKKSIGRN 300
           GKKK+GDGSLSSSNSTSRSLEMN+SAEEDI ALVPEVGSRTP+TRVKRVAIADKKSIGRN
Sbjct: 241 GKKKDGDGSLSSSNSTSRSLEMNISAEEDITALVPEVGSRTPKTRVKRVAIADKKSIGRN 300

Query: 301 GLKSQTHPIKCKPDPSNNEDVEEKTLYMIEPSTKNETEEMSQNSVHTTESSQPQSSSTID 360
           GLKSQ H IKCKPDPSNNEDVEEKTLYMIEPS+KNETE MSQ+S+HTTESSQPQSSST D
Sbjct: 301 GLKSQIHHIKCKPDPSNNEDVEEKTLYMIEPSSKNETEGMSQSSLHTTESSQPQSSSTTD 360

Query: 361 NNLKHEQEAAANSIVPPMSVKKNAVKRARNGPSSKILSTSPTASKVFKGIRPKRFGMVQR 420
           NNLKHEQEA ANSIVPPMS KKN VKRARNG S KILSTSPT   VFKGIRPKRFGMVQR
Sbjct: 361 NNLKHEQEAVANSIVPPMSEKKNVVKRARNGTSPKILSTSPT---VFKGIRPKRFGMVQR 420

Query: 421 SETRSAPSSPLSSRFQSEPIHVEHRGSTSGNDVKKSENSKVDHRLKTIRMTLTDSENGDC 480
           SETRSAPSSPLSSR QSEPIHVEHRGSTSGN VKK ENSKVDHRLKT RMTLTDSENGDC
Sbjct: 421 SETRSAPSSPLSSRSQSEPIHVEHRGSTSGNGVKKIENSKVDHRLKTRRMTLTDSENGDC 480

Query: 481 QSRKLKFRKGKTVELQPETSSPRRLKFRRVRLLGETQSPKGDSRKRNIKGKEGNQNGKE- 540
           QSRKLKFRKG+ VELQPETS+PRRLKFRRVRLLGE +SPKGDSRKRNIKGKEGNQNGKE 
Sbjct: 481 QSRKLKFRKGRMVELQPETSTPRRLKFRRVRLLGEAESPKGDSRKRNIKGKEGNQNGKEV 540

Query: 541 --GENSSLRQQDKDLKKKRSFRDGKLISSRFKSERVVLRHQDSKGKKEILNLFNNVIEET 600
             GENSSLRQQDKDLKKKRSFRDGKL+SSRFKSERVVLRHQDSKGKKE+LNLFNNVIEET
Sbjct: 541 KEGENSSLRQQDKDLKKKRSFRDGKLVSSRFKSERVVLRHQDSKGKKEVLNLFNNVIEET 600

Query: 601 ASKLAKTKKSKVKALVGAFETVISLQDTKPAATTSVA 634
           ASKLAKT+KSKVKALVGAFETVISLQDTKP ATTSVA
Sbjct: 601 ASKLAKTRKSKVKALVGAFETVISLQDTKPTATTSVA 619

BLAST of Chy12G221190 vs. NCBI nr
Match: XP_038894083.1 (uncharacterized protein LOC120082825 [Benincasa hispida] >XP_038894084.1 uncharacterized protein LOC120082825 [Benincasa hispida])

HSP 1 Score: 861 bits (2224), Expect = 5.59e-308
Identity = 492/630 (78.10%), Postives = 533/630 (84.60%), Query Frame = 0

Query: 11  AMEEVSEAEVSQEESFDIPVIAVANTSEPEDITEEIIDIIDIIDIPATIEVNEPDIIDIP 70
           AMEEVSE E SQEESFDIPVIAVA TSEPEDI EE IDIIDI   PA             
Sbjct: 41  AMEEVSEDEASQEESFDIPVIAVAETSEPEDIIEENIDIIDI---PAI------------ 100

Query: 71  ATIEVSEPESCKVEVIVDINSNTPKIRPRVLSRYLRPHTGSCHDFCKYGSKHALEGKPAS 130
            TIE++EPES  VEVIVDINS+TPKI P++LSRYL PHTGSCHDFCKYG+KH LEGKPAS
Sbjct: 101 TTIELNEPESRSVEVIVDINSSTPKIMPKILSRYLFPHTGSCHDFCKYGTKHGLEGKPAS 160

Query: 131 PVSRKAKLVEGNGQDLRRIVVSLAKQNKESNSRKSSPEYNPNNVTD-LKEDVISSPEIIT 190
            V RK K   G+G+ LRRI+VS AKQNK++ S KSSPE+NP NVT  LKED+IS PEI+T
Sbjct: 161 TVLRKVKSAGGDGRGLRRIIVSSAKQNKDATSPKSSPEHNPINVTGHLKEDIISPPEIVT 220

Query: 191 PSPKRLLPSTKEVQAAAVHYSRTKLNLSLSKVSSFAEQGSSRTKRNKEIRKGKKKEGDGS 250
           PSPKRLLPS KEVQAAAVHYSRTKLNLSLSK SSFA QGSSRTKRNKEIR+G KK+GDGS
Sbjct: 221 PSPKRLLPSIKEVQAAAVHYSRTKLNLSLSKASSFAGQGSSRTKRNKEIRRGNKKDGDGS 280

Query: 251 LSS-SNSTSRSLEMNVSAEEDIPALVPEVGSRTPRTRVKRVAIADKKSIGRNGLKSQTHP 310
            SS +NSTSRS EMN+SAEEDI ALVPEV S TPR RVKRVAI DKK IGR+GLKSQ+H 
Sbjct: 281 SSSCTNSTSRSQEMNISAEEDIKALVPEVVSWTPRNRVKRVAIPDKKIIGRSGLKSQSHS 340

Query: 311 IKCKPDPSNNEDVEEKTLYMIEPSTKNETEEMSQNSVHTTESSQPQSSSTIDNNLKHEQE 370
           IKCKPDPSNNEDVEEKTLYMIEPSTKNETEEM+QNSVH TESS+PQSSS  DN+LKHE+E
Sbjct: 341 IKCKPDPSNNEDVEEKTLYMIEPSTKNETEEMAQNSVHATESSRPQSSSATDNSLKHEKE 400

Query: 371 AAANSIVPPMSVKKNAVKRARNGPSSKILSTSPTASKVFKGIRPKRFGMVQRSETRSAPS 430
              N  +PP+SVKKN V+ ARN  SSKI S SP  SKVFKGIRPKRFGMVQRSETR APS
Sbjct: 401 VDENLKMPPLSVKKNVVRCARNVTSSKISSASPPVSKVFKGIRPKRFGMVQRSETRLAPS 460

Query: 431 SPLSSRFQSEPIHVEHRGSTSGNDVKKSENSKVDHRLKTIRMTLTDSENGDCQSRKLKFR 490
           SPLSSR   EP+HVEHRGSTSGN+VKK ENS+V HRLKT RMTLTDSENGD QSRKLKFR
Sbjct: 461 SPLSSRPPFEPVHVEHRGSTSGNEVKKRENSEVKHRLKTKRMTLTDSENGDSQSRKLKFR 520

Query: 491 KGKTVELQPETSSPRRLKFRRVRLLGETQSPKGDSRKRNIKGKEGNQNG---KEGENSSL 550
           KG+ VELQ ET++PRRLKFRRV LLGETQSPKGD RKRNIKGKE NQNG   KE +NSSL
Sbjct: 521 KGRVVELQAETNTPRRLKFRRVHLLGETQSPKGDPRKRNIKGKEANQNGNEVKEADNSSL 580

Query: 551 RQQDKDLKKKRSFR-----DGKLISSRFKSERVVLRHQDSKGKKEILNLFNNVIEETASK 610
           RQQD++LKKKRSFR     DGKL+SSR KSERVVLRHQDS+GKK I NLFNNVIEETASK
Sbjct: 581 RQQDQELKKKRSFRRKETIDGKLVSSRIKSERVVLRHQDSRGKKAIQNLFNNVIEETASK 640

Query: 611 LAKTKKSKVKALVGAFETVISLQDTKPAAT 630
           LA+T+KSKVKALVGAFETVISLQDT+P AT
Sbjct: 641 LAQTRKSKVKALVGAFETVISLQDTRPVAT 655

BLAST of Chy12G221190 vs. NCBI nr
Match: KAG7020011.1 (hypothetical protein SDJN02_18979, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 686 bits (1770), Expect = 6.48e-237
Identity = 450/817 (55.08%), Postives = 514/817 (62.91%), Query Frame = 0

Query: 1   MANENSDIPLAMEEVSEAEVSQEESFDIPVIAVANTSEPEDITEEIIDIIDIIDIPATIE 60
           MA +N+DIPLAMEEVS  E  Q+ESFDIPVI VA TS+P+DITEE IDI     + A  E
Sbjct: 1   MAEQNNDIPLAMEEVSGDEARQDESFDIPVIEVAETSKPDDITEESIDI----PVIAVAE 60

Query: 61  VNEP--------DIIDIPATIEVSEPESCKVEVIVDINSNTPKIRPRVLSRYLRPHTGSC 120
            +EP        DIIDIPAT EV+EPESC VEVIV IN  TPK++P++LSRYL PHTGSC
Sbjct: 61  TSEPEDITEKGIDIIDIPATTEVNEPESCNVEVIVHIN--TPKMKPKILSRYLLPHTGSC 120

Query: 121 HDFCKYGSKHALEGKPASPVSRKAKLVEGNGQDLRRIVVSLAKQNKESNSRKSSPEYNPN 180
           HDFCKYG+K  LEGKPA  + RKAK + G G+DLRRI+V LAKQN  + S KSSP+YN  
Sbjct: 121 HDFCKYGAKQDLEGKPARSILRKAKSMGGGGRDLRRIMVLLAKQN--TISPKSSPDYNAI 180

Query: 181 NVTDLKEDVISSPEIITPSPKRLLPSTKEVQAAAVHYSRTKLNLSLSKVSSFAEQGSSRT 240
           N+TD+KED+IS PEI+TPSPKR LPS KEVQAA+VHY RTKLNLS SK SSFA Q SSRT
Sbjct: 181 NITDVKEDIISFPEIVTPSPKRPLPSIKEVQAASVHYRRTKLNLSRSKASSFARQVSSRT 240

Query: 241 KRNKEIRKGKKKEGDGSLSS-SNSTSRSLEMNVSAEEDIPALVPEVGSRTPRTRVKRVAI 300
           KRNKE+RK KK++GDGS SS +NSTSR  E N+SAEED+  LVP V  R PR RVKRV I
Sbjct: 241 KRNKELRKDKKQDGDGSSSSCTNSTSRCQERNISAEEDMKDLVPRVLFRVPRNRVKRVTI 300

Query: 301 ADKKSIGRNGLKSQTHPIKCKPDPSNNEDVEEKTLYMIEPSTKNETEEMSQNSVHTTES- 360
           ADKK  GR GLK   HP K KPDP NNEDVEEKTLYMIEPSTKNETEE++QNSV T ES 
Sbjct: 301 ADKKITGRCGLKRPKHPRKYKPDPFNNEDVEEKTLYMIEPSTKNETEELAQNSVQTAESR 360

Query: 361 ------------------------------------------------------------ 420
                                                                       
Sbjct: 361 SQSSSATDNSLKHEQESDGSSVLPPLSVKKNMRHARNRTSPKILSTKNETEELTQKCIQT 420

Query: 421 --SQPQSSSTIDNNLKHEQEAAANSIVPPMSVKKNAVKRARNGPSSKILST--------- 480
             S+PQSSS  DN+LKHEQE+  + IVPP+SVKKN V+RAR   S+KILST         
Sbjct: 421 AESRPQSSSATDNSLKHEQESDGSPIVPPLSVKKN-VRRARKRTSTKILSTKNEIEELTQ 480

Query: 481 ---------------------------------------------SPTASKV-------- 540
                                                        S T+ K+        
Sbjct: 481 NSVQTAESRLQYSSATDNSLKHEQESDGAPIVSPLSVKKNVRRSRSRTSPKILSTKNETE 540

Query: 541 ------------------------------------------------FKGIRPKRFGMV 600
                                                           FKGIR KRFGM 
Sbjct: 541 ELAQNSVQTAESRPQSSFATDNRLKHQLESGGAPIVSPWSVKKNVRREFKGIRHKRFGMA 600

Query: 601 QRSETRSAPSSPLSSRFQSEPIHVEHRGSTSGNDVKKSENSKVDHRLKTIRMTLTDSENG 629
             +ETRSAPSSP SSR+ SE +HVEHRGSTSGND+KKSENSKV+ +LKT RM L+DS  G
Sbjct: 601 HMTETRSAPSSPFSSRYPSERVHVEHRGSTSGNDMKKSENSKVELKLKTRRMALSDS--G 660

BLAST of Chy12G221190 vs. NCBI nr
Match: XP_022998278.1 (uncharacterized protein LOC111492963 [Cucurbita maxima])

HSP 1 Score: 641 bits (1654), Expect = 4.54e-222
Identity = 402/639 (62.91%), Postives = 463/639 (72.46%), Query Frame = 0

Query: 1   MANENSDIPLAMEEVSEAEVSQEESFDIPVIAVANTSEPEDITEEIIDIIDIIDIPATIE 60
           MA EN+DIPL +E VS  E  Q ESFDIP     N  EP  I E   DI   ++  A  E
Sbjct: 1   MAGENTDIPLVVE-VSGDEGRQHESFDIPTNMEVN--EPNAIIEGSTDISLALEASAD-E 60

Query: 61  VNEPDIIDIPATIEVSEPESCKVEVIVDINSNTPKIRPRVLSRYLRPHTGSCHDFCKYGS 120
            ++ +  DIP T+EV+EPESC V VIV+IN  TPK R R+  RYL P TGSCHDFCKYG+
Sbjct: 61  GSQDEKFDIPTTMEVNEPESCNVGVIVNIN--TPKFR-RIFPRYLSPRTGSCHDFCKYGT 120

Query: 121 KHALEGKPASPVSRKAKLVEGNGQDLRRIVVSLAKQNKESNSRKSSPEYNPNNVTDLKED 180
           KH +E  PAS V RK K V  + +DLRRI V+LAK N ++ S K S +Y+  N+TDLKED
Sbjct: 121 KHPIERNPASAVLRKGKSVGRDVRDLRRIRVTLAKPNNDTVSPKFSKDYDGINITDLKED 180

Query: 181 VISSPEIITPSPKRLLPSTKEVQAAAVHYSRTKLNLSLSKVSSFAEQGSSRTKRNKEIRK 240
           V SSPEII PSPK+ LP  KEV+AAAV YSRTKLNLSLSK SS A Q +SRT RNKE+R+
Sbjct: 181 VTSSPEIINPSPKKRLPPIKEVEAAAVQYSRTKLNLSLSKASSSAGQCTSRTNRNKEVRR 240

Query: 241 GKKKEGDGSLSSS-NSTSRSLEMNVSAEEDIPALVPEVGSRTPRTRVKRVAIADKKSIGR 300
            KK++G GS SSS +STSR  E+ +S   D  ALVP   S TPR RVKRVAI DKK IGR
Sbjct: 241 SKKQDGYGSSSSSTDSTSRCQEIKISTVGDRKALVPHAVSWTPRNRVKRVAIVDKKIIGR 300

Query: 301 NGLKSQTHPIKCKPDPSNNEDVEEKTLYMIEPSTKNETEEMSQNSVHTTESSQPQSSSTI 360
           + LK+Q+   K KPDPSN+E VEEKTLYMIEPSTK ETE  +QNSVH TE S PQSSS  
Sbjct: 301 HSLKNQSRLKKIKPDPSNSEVVEEKTLYMIEPSTKKETEGPAQNSVHFTELSHPQSSSAT 360

Query: 361 DNNLKHEQEAAANSIVPPMSVKKNAVKRARNGPSSKILSTSPTASKVFKGIRPKRFGMVQ 420
           DN+ KH+QEA    I P + V++N  +R RNG SSK LSTSPT  K FKG+RPKRF M+Q
Sbjct: 361 DNSFKHKQEADGTIITPHLLVEENT-RRTRNGTSSKSLSTSPTVFKGFKGLRPKRFDMIQ 420

Query: 421 RSETRSAPSSPLSSRFQSEPIHVEHRGSTSGNDVKKSENSKVDHRLKTIRMTLTDSENGD 480
            SETRSAPSSP SSR  SEP+H               E SKV+H++K  R TLTDSENGD
Sbjct: 421 MSETRSAPSSPSSSRCSSEPVH--------------GEKSKVEHKIKARRKTLTDSENGD 480

Query: 481 CQSRKLKFRKGKTVELQPETSSPRRLKFRRVRLLGETQSPKGDSRKRNIKGKEGNQNG-- 540
           CQSRKL FRKG+ VELQ E  +PRRL F+RVR L ETQSPK DSRKR I+ KE NQNG  
Sbjct: 481 CQSRKLHFRKGRMVELQTEPITPRRLIFKRVRSLSETQSPKSDSRKRIIQRKEANQNGDE 540

Query: 541 -KEGENSSLRQQDKDLKKKRSFR-----DGKLISSRFKSERVVLRHQDSKGKKEILNLFN 600
            KE ENSSLRQQD++ K+K+SFR     DGKL+SSR KSER+VL+HQDS  K EI  L N
Sbjct: 541 VKETENSSLRQQDQEFKRKKSFRRQETIDGKLVSSRLKSERIVLKHQDSNEKIEIQKLLN 600

Query: 601 NVIEETASKLAKTKKSKVKALVGAFETVISLQDTKPAAT 630
           NVIEETA+KLAKT+KSKVKALVGAFETVISLQD KPAAT
Sbjct: 601 NVIEETATKLAKTRKSKVKALVGAFETVISLQDAKPAAT 617

BLAST of Chy12G221190 vs. TAIR 10
Match: AT5G07820.1 (Plant calmodulin-binding protein-related )

HSP 1 Score: 93.6 bits (231), Expect = 6.2e-19
Identity = 169/624 (27.08%), Postives = 272/624 (43.59%), Query Frame = 0

Query: 20  VSQEESFDIPVIAVANTSEPEDITEEIIDIIDIIDIPATIEVNEPDIIDIPATIEVSEPE 79
           +S+E    +      + + P +I+ E I ++D        EV   D +  PA  E +   
Sbjct: 1   MSEENISQVDDKCCLDDANPMEISVETIPVVD-------EEVQGDDDVLHPAA-ETNHAS 60

Query: 80  SCKVEVIVDINSNTPKIRPRVLSRYLRPHTGSCHDFCKYGSKHA--LEGKPASPVSRKAK 139
             +    V ++  T K   +V +RY    T S HD CK+G +    L  KP   V++K  
Sbjct: 61  LKRYSTRV-LDKKTGK--AQVQTRYRGNQTSSTHDLCKHGKRREEDLVIKPWK-VAKKKN 120

Query: 140 LVEGN---GQDLRRIVVSLAKQNKES-NSRKSSPEYNPNNVTDLKEDVISSPEIITPSPK 199
           +  G+   G+ LR+ + +++K +K S  + K           D      S  +  +PS  
Sbjct: 121 VDSGDLGKGETLRKSLGNVSKPDKSSLQAAKREAAGEVVKSCDGLHVKKSETKSTSPSVS 180

Query: 200 RLLPSTKEVQAAAVHYSRTKLNLS----LSKVSSFAEQGS--SRTKRNKEIRKGKKKEGD 259
            ++ + K+        SR   N S    LSK     E+G      + +  + K   +   
Sbjct: 181 PVVRTVKKTNLVVNKASRISQNKSPKEDLSKNLKNKEKGKIVEPVRCDDVLEKTDLEVKK 240

Query: 260 GSLSSSNSTSR--SLEMNVSAEEDIPALVPEVGSRTPRTRVKRVAIADKKSIGRNGLKSQ 319
            S  S N +S+  +L+    A+ D P    +V  +T     K   I++ K+     LK+ 
Sbjct: 241 VSRISENKSSKEDTLKNKEKAKIDEPVRCDDVLEKTSLDAQKVSRISENKNSKEERLKNL 300

Query: 320 THPIKCKPD-PSNNEDVEEKTLYMIEPSTKNETEEMSQNSVHTTESSQPQSSSTIDNNLK 379
            +  K   D P   +D  EKTLY++E S + + ++MS  SV  +E+ Q      I     
Sbjct: 301 KNKEKTNIDEPVRPDDAVEKTLYVVESSVEKKKKKMSTKSVKISETQQSSEKKII----- 360

Query: 380 HEQEAAANSIVPPMSVKKNAVKRARNGPSSKILSTSPTASKVFKGIRPKRFGMVQRSETR 439
                                 R+    S  +L + P  S+V  G  P+    ++++ +R
Sbjct: 361 ----------------------RSTGKKSLSLLPSLPPPSEVVTGSDPR---PIRQTTSR 420

Query: 440 SAPSSPLSSRFQSEPIHVEHRGSTSGNDVKKSENSKVDHRLKTIRMTLTDSENGDCQSRK 499
           S  S P            E + S S N V    N K + +++  R+ L  +       ++
Sbjct: 421 SKTSLP------------EKKQSGSANLV---TNPKPESKIRPKRIGLKVTPPPPPTKQQ 480

Query: 500 LKFRKGKTVELQPETSSPRRLKFRRVRLLGETQSPKGDSRKRNIKGKEGNQNGKEGENSS 559
           + F+KGK +E +PE S+   +KF+++      Q PK                        
Sbjct: 481 MNFKKGKVLEPKPEDSTTTSIKFKKI----VVQEPK------------------------ 538

Query: 560 LRQQDKDLKKKRSFRDGK----LISSRFKSERVVLRHQDSKGKKEILNLFNNVIEETASK 619
           LR  D + KKK+S +D +     I+   K E+VVLRH+  + KK++  LFNNVIEET +K
Sbjct: 541 LRTSDVN-KKKKSLKDKREGVGKINGEGKREKVVLRHRKVEVKKKLQTLFNNVIEETVNK 538

Query: 620 LAKTKKSKVKALVGAFETVISLQD 625
           L + +KSKVKALVGAFETVISLQD
Sbjct: 601 LEEVRKSKVKALVGAFETVISLQD 538

BLAST of Chy12G221190 vs. TAIR 10
Match: AT5G39380.1 (Plant calmodulin-binding protein-related )

HSP 1 Score: 91.3 bits (225), Expect = 3.1e-18
Identity = 159/584 (27.23%), Postives = 238/584 (40.75%), Query Frame = 0

Query: 93  TPKIRPRVLSRYLRPHTGSCHDFCKYGSKHALEGKP------------------------ 152
           T K + + +  YLR  TGSCHD CKYG +     KP                        
Sbjct: 34  TSKPKEKKIPHYLRASTGSCHDLCKYGKRQIPVEKPWRSSTKKIFKKSLDDNLNETLKPG 93

Query: 153 ASPVSRKAKLVEGN-GQD-----LRRIVVSLAKQNKESNSRK------SSPEYNPNNVTD 212
           +S + +K + VE N G D     ++R VV        S  RK      SS +  P  V  
Sbjct: 94  SSKMKKKVREVEKNEGTDDSFEVIKREVVKYQASGVSSGMRKPEVLIISSCDETP--VKQ 153

Query: 213 LKEDVISSPEIITPSPKRLLPSTKEVQA---AAVHYSRTKLNLSLSKVSSFAEQGSSRTK 272
           +K+    S + + PSP     S++ V A     +  S + L  S  KV +  +  +S   
Sbjct: 154 IKKKTTLSSK-LKPSPDLGSRSSENVDALKPKVLKKSYSALTTSKPKV-NHEKVVASPVL 213

Query: 273 RNKEIRKGKKKEGDGSLSSSNSTSRSLEMNVSAEEDIPALVPEVGSRTPRTRVKRVAIAD 332
           + K  ++   K+ DG +     +SR     V++++   A V    S +PR  V+    + 
Sbjct: 214 KPKMGKRNDGKDEDGKVKKGTVSSR-----VASKK---APVTPRASLSPRLSVRLAGSSS 273

Query: 333 KKSIGRNGLKSQTHPIKCKPDPSNNED----------VEEKTLYMIEPSTKNETEEMSQN 392
            +        S +     KP P N  D          VEEKTL+++E  T N    +S+N
Sbjct: 274 LRKSQSLKAASSSSRQNQKPRPVNRTDEFNKQLDDYPVEEKTLHVVEMETTNNV--VSEN 333

Query: 393 SVHTTESSQPQSSSTIDNNLKHEQEAAANSIVPPMSVKKNAVKRARNGPSSKILSTSPTA 452
                           D N    Q+      +PP+                         
Sbjct: 334 ----------------DQN----QQGFVEPFLPPL------------------------- 393

Query: 453 SKVFKGIRPKRFGMVQRSETRSAPSSPLSSRFQSEPIHVEHRGSTSGNDVKKSENSKV-- 512
                              T+S P     +  ++E         TSG++  +SE  ++  
Sbjct: 394 -----------------PPTQSTPKDDECTVSETEEYEY-----TSGSNEAESEEEEIGL 453

Query: 513 DHRLKTIRMTLTDSENGDCQSRKLKFRKGKTVELQPETSSPRRLKFRRVRLLGETQSPKG 572
            +  K  R    + ++ D  +RKL+FR+G  V+        R+LKFRR R LGE ++   
Sbjct: 454 SNGEKKPRAARKEGDSADEAARKLRFRRGTIVDPDTVGEKARKLKFRRGRGLGEDKAQDA 507

Query: 573 DSRKRNIKGKEGNQNGKEGENSSLRQQDKDLKKKRSFRDGKLISSRFKSERVVLRHQDSK 626
             R R+ K +E                  D++++    DG         E+VVLRHQD +
Sbjct: 514 QVR-RSFKKRE------------------DIREEEVNEDG---------EKVVLRHQDVQ 507

BLAST of Chy12G221190 vs. TAIR 10
Match: AT5G61260.1 (Plant calmodulin-binding protein-related )

HSP 1 Score: 89.7 bits (221), Expect = 8.9e-18
Identity = 158/631 (25.04%), Postives = 273/631 (43.26%), Query Frame = 0

Query: 20  VSQEESFDIPVIAVANTSEPEDITEE-------IIDIIDIIDIPATIEVNEPDIIDIPAT 79
           +++E + ++  I  ++ + P  ++EE       + D+ ++ D+ A +  ++ +I  IP  
Sbjct: 1   MAEENTSEVKDIENSSDTNPPKMSEEGSLETVRVKDVENVEDVIAVVAKDQEEI--IPEI 60

Query: 80  IEVSEPESCKVEVIVDINSNTPKIRPRVLSRYLRPH-TGSCHDFCKYGSKHALEGKPASP 139
           IEV +P+   +      +S     +P+V SRY   H  GS HD CK+G K   +      
Sbjct: 61  IEVPKPQGGTLR----RHSTGTISKPQVQSRYRGNHRVGSTHDLCKHGKKCDQDDAVKPW 120

Query: 140 VSRKAKLVEGNGQDLRRIVVSLAKQNKESNSRKSSPEYNPNNVTDLKEDVISSPEIITPS 199
              + K VEG+      +V+ +   +    S  S    +P          I+ P+    +
Sbjct: 121 KIARRKSVEGS------VVIKVETPSSTRKSLGSVSRQSPG---------ITKPDSSVSA 180

Query: 200 PKRLLPSTKEVQAAAVHYSRTKLNLSLSK-VSSFAEQGSSRTKRNKEIRKGKKKEGDGSL 259
            +  L   K+  A+    S +K    ++K V   + + + R ++NKE   G        L
Sbjct: 181 KRDALAVKKKPCASVNSESSSKEGSEIAKSVDGLSVKSNDRARKNKETESG--------L 240

Query: 260 SSSNSTSRSLEMNVSAEEDIPALVPEVGSRTPRTRVKRVAIADKKSIGRNGLKSQTHPIK 319
           S S           +  + +PAL  +  S +  T V    +   K++ +N  K++T    
Sbjct: 241 SGS-----------AVVKKVPALRTDKSSTS--TGVGSSKVCAPKNL-KNVEKAKT---- 300

Query: 320 CKPDPSNNEDVEEKTLYMIEPSTKNETEEMSQNSVHTTESSQPQSSSTIDNNLKHEQEAA 379
                 + EDV+EKT+ ++E S K    E   +S   T  S  +S ST        +  +
Sbjct: 301 --TQTISGEDVKEKTVCVVESSVKGVKSEKQPSSEKKTMKSGNKSLSTT------PKRGS 360

Query: 380 ANSIVPPMSVKKNAVKRARNGPSSKILSTSPTASKVFKGIRPKRFGMVQRSETRSAPSSP 439
           + +   P  +     K+   G S+ ++  +P   K  K +RPK+ G              
Sbjct: 361 SPTKQIPGKISTGLTKKKETG-SADVVEANP---KPEKKVRPKKTG-------------- 420

Query: 440 LSSRFQSEPIHVEHRGSTSGNDVKKSENSKVDHRLKTIRMTLTDSENGDCQSRKLKFRKG 499
                                                ++++L         ++++ F+KG
Sbjct: 421 -------------------------------------VKVSL---------AQQMTFKKG 480

Query: 500 KTVELQPETSSPRRLKFRRVRLLGETQSPKGDSRKRNIKGKEGNQNGKEGENSSLRQQDK 559
           K ++ +PE SSPR +KF++ R++ E ++                    EG+  +L+ +  
Sbjct: 481 KVLDPKPEDSSPRWIKFKK-RVVQELKTQ------------------SEGKKKNLKDRRL 487

Query: 560 DLKKKRSFRDGKLISSRFKSERVVLRHQDSKGKKEILNLFNNVIEETASKLAKTKKSKVK 619
            ++ K    +G       K E+VVLRH+  +GKK+++ LFNNVIEET +KL K +K KVK
Sbjct: 541 GVETKTDSCEGS------KREKVVLRHRKVEGKKKMITLFNNVIEETVNKLTKVRKHKVK 487

Query: 620 ALVGAFETVISLQDT-----KP--AATTSVA 635
           AL+GAFETVISLQDT     KP   ATTS +
Sbjct: 601 ALIGAFETVISLQDTNKTSHKPQSKATTSAS 487

BLAST of Chy12G221190 vs. TAIR 10
Match: AT5G15430.1 (Plant calmodulin-binding protein-related )

HSP 1 Score: 80.5 bits (197), Expect = 5.4e-15
Identity = 145/564 (25.71%), Postives = 236/564 (41.84%), Query Frame = 0

Query: 99  RVLSRYLRPHTGSCHDFCKYGSKHALEGKPASP----VSRKAKLVEGNGQDLRR--IVVS 158
           +V+  YLR  TGSCHD CKYG K   E KP  P    VSR           LR+  +   
Sbjct: 34  KVVPNYLRSPTGSCHDACKYGRKDESEDKPRVPHRKRVSRSFSGAINLDSPLRKKALTKP 93

Query: 159 LAKQNKESNS------RKSSPEYNPNNVTDLKEDVI--SSPEIITPSPKRLLPST--KEV 218
           L   ++  +S       KS      + V D+K+     ++ ++++ S  RL  ST  K+ 
Sbjct: 94  LLSPSRRCDSVGGFDHAKSQVRNFSSGVCDVKKSHADGTNEKVVSVSESRLADSTKRKKK 153

Query: 219 QAAAVHYSR--------TKLNLSLSKVSSFAEQGSSRTKRNKEIRKGKKKEGDGSLSSSN 278
           +   V+ SR         K  ++  K+ + A+      +R+  +++ K   G  +     
Sbjct: 154 KKKTVYVSRGRAKEIVEQKRRVTALKLKAVAQTAEIALRRS-TVKRKKMNGGSKAAEQKK 213

Query: 279 STSRSLEMNVSAEEDIPALVPEVGSRTPRTRVKRVAIADKKSIGRNGLKSQTHPIKCKPD 338
           +       ++S++     L  +  S +    +K+     +K +G           KCK  
Sbjct: 214 AVMALRRASMSSKGCSRCLKTKKESNSLSVPLKKT----RKHVGD----------KCK-- 273

Query: 339 PSNNEDVEEKTLYMIEPSTKNE--TEEMSQNSVHTTESSQPQSSSTIDN----NLKHEQE 398
               + VEEKTLY+I+  T +E    E++Q  V  +    P+S  + D       +HE E
Sbjct: 274 ----DLVEEKTLYVIKMETVDEIVESELNQRCVMDSPIDDPKSEKSQDKGECIETEHEDE 333

Query: 399 AAANSIVPPMSVKKNAVKRARNGPSSKILSTSPTASKVFKGIRPKRFGMVQRSETRSAPS 458
           ++          +++  +   N                           V  SE ++   
Sbjct: 334 SSQE--------EEDEEEEDEN---------------------------VSVSEDKNTTR 393

Query: 459 SPLSSRFQSEPIHVEHRGSTSGNDVKKSENSKVDHRLKTIRMTLTDSENGDCQSRKLKFR 518
              S  F +E        + +GN                              + KL+ R
Sbjct: 394 EGKSKAFSAE-------SAITGN------------------------------AMKLRIR 453

Query: 519 KGKTVELQPETSSPRRLKFRRVRLLGETQSPKGDSRKRNIKGKEGNQNGKEGENSSLRQQ 578
           +GK ++   E +SPR+LKF+R +++    +      +R +K K        G N S    
Sbjct: 454 RGKIIDFGSEGNSPRKLKFKRGKIISGADTTSKSGGRRRLKTK--------GTNLS---N 478

Query: 579 DKDLKKKRSFRDGKLISSRFKSERVVLRHQDSKGKKEI-LNLFNNVIEETASKLAKTKKS 632
           DK+ ++K                RVVL+HQD++ K+E  + LFN VI+ETA+KL +T+KS
Sbjct: 514 DKEQQRK---------------PRVVLKHQDTEKKRESRVLLFNKVIKETANKLVQTRKS 478

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A0A0LST40.0e+0095.11CaM_binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G038960... [more]
A0A1S3BFX35.6e-29489.01uncharacterized protein LOC103489380 OS=Cucumis melo OX=3656 GN=LOC103489380 PE=... [more]
A0A5D3BIK35.6e-29489.01CaM_binding domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A6J1KGB04.6e-17962.91uncharacterized protein LOC111492963 OS=Cucurbita maxima OX=3661 GN=LOC111492963... [more]
A0A6J1KLU33.0e-17847.41uncharacterized protein LOC111495740 OS=Cucurbita maxima OX=3661 GN=LOC111495740... [more]
Match NameE-valueIdentityDescription
XP_011650019.10.095.11uncharacterized protein LOC105434693 [Cucumis sativus] >XP_011650022.1 uncharact... [more]
XP_008446785.10.089.01PREDICTED: uncharacterized protein LOC103489380 [Cucumis melo] >XP_008446868.1 P... [more]
XP_038894083.15.59e-30878.10uncharacterized protein LOC120082825 [Benincasa hispida] >XP_038894084.1 unchara... [more]
KAG7020011.16.48e-23755.08hypothetical protein SDJN02_18979, partial [Cucurbita argyrosperma subsp. argyro... [more]
XP_022998278.14.54e-22262.91uncharacterized protein LOC111492963 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT5G07820.16.2e-1927.08Plant calmodulin-binding protein-related [more]
AT5G39380.13.1e-1827.23Plant calmodulin-binding protein-related [more]
AT5G61260.18.9e-1825.04Plant calmodulin-binding protein-related [more]
AT5G15430.15.4e-1525.71Plant calmodulin-binding protein-related [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012417Calmodulin-binding domain, plantSMARTSM01054CaM_binding_2coord: 481..621
e-value: 1.2E-23
score: 94.6
IPR012417Calmodulin-binding domain, plantPFAMPF07839CaM_bindingcoord: 542..621
e-value: 5.6E-23
score: 81.9
coord: 484..555
e-value: 1.4E-4
score: 22.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 438..470
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 248..263
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 384..557
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 330..359
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 155..179
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 313..329
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 155..194
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 479..493
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 543..557
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 501..536
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 230..247
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 220..359
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 421..436
NoneNo IPR availablePANTHERPTHR33349EMB|CAB62594.1coord: 66..630
NoneNo IPR availablePANTHERPTHR33349:SF1EMB|CAB62594.1coord: 66..630

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy12G221190.1Chy12G221190.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005516 calmodulin binding