Chy12G220980 (gene) Cucumber (hystrix) v1

Overview
NameChy12G220980
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionExocyst subunit Exo70 family protein
LocationchrH12: 18822797 .. 18824713 (-)
RNA-Seq ExpressionChy12G220980
SyntenyChy12G220980
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGGGATTGTGAATCAATTATACCAACCTCTGAAGGGGAGCAACACGCTCTAGCTGCATTTCAGCACCTAGTCAAGTTCTTAAGGACAAGTCAGAACGTGAATAATGAATTGAAAAAGCTCCTTGCCGATCTCGATTCTCATTTGACTTCCTTTACGTCGTATACTCATTTAGAGGTTGGAAAGTTGAGTGAGTTAGAGACAAGGTTCAAATCTGCCGAGGAGAAGATTACGAGATGGGAATCTAACAAATCCATGATATGGGATTCTGATCCCAAAGAGGCTTGTGAATATATGAAGGCTGTTGATGAAATTCATAAAGTTCAGGAAGGTCTAAGAAGTTTATCTGCAAATGACAGCCAAAAGCAAAATGAACTTCTTTTCCAGGCCGACTCTGTCGTACAGATTGCAATGGCAAGGCTAGAGCAGGAGCTAATCCACATTCTTGCTCAGCACAAACTATATTTTGAGCCTGACTATGTTCCCTTCCAGTCTGGTGGCTACGATGTTGTCTATAGCGAGTCCTTTGTTTCAGCTGAGGACAGTTTGGAGGTTGAAACTTCCCACGGAGAGAGTATTCCTGCTTTATATGCTGTGAACTCGGTCCATCCACACGTGATCGCTCATCTTAAATCCATCGCAAATGTTATGTTTACTTCAAATTATATTCAGGAATTCTGCCTGACTTTTGTTAAAATGCGGAAAGATGCGTTGAACGAGTATCTGTTCATTCTCGAAATGGAGAAGTATAGCATTGGAGATTTGCTGACCATGGAATGGAGCGACTTAAACGTGAAGATCAAGAAATGGGTTTGGCTTATTAAAATTGTTATTAGAGTCTACCTTATTAGTGAGCAAAGACTCTGTAACAATATATTTGAAGGCATTGGATCTTATAGTGCAGTCTGCTTTACTGAGATTTCAGCGACTTCTATGCTTCGCCTGTTGAATTTTGGAGAGGCCATAGCTATGGAACCCCATCGACCGGAAAAGCTGTTTCACTTGCTAGACATGTATGAGGTCCTAAAAAATCTTCTTGGATTCGTAGATGAATTGTTTTCCGAAGAAACAGAAAAGGGTTCTTTTCTCAAATTTGAGTTCCATAATCTTTTCAAGAAATTGGGTGATTCTGCAAGGGCTACGTTTTTAGATTTTGGAAATTTCATTGCATGTTCCATATCAACAAACCCTTTTCCCGGCGGTGGTGTTCATCATCTAACAAGGTATGTAATGAACTACATCCATACCCTTACTGTTTTCAGAGACTCGCTTGTTATCCTTCTCCAAGATCAAGCAGCAGATGACTTAAGCCCAACTTCTGAGCTGCAGAGTGAAATCAATAGCATACCTTGCCCAATGGCTTACCACCTTCAGTCAATCATTTCACATTTGCTATCCAATCTCAATAACAAATCCAAGTTGTACAAGGATGGTGCTCTACGACATATTTTCTTAATGAACAACATCCATTATATAGTTCAAAAGGTTGAAAACTCTGATCTCATAGCCTTTCTTGGAAGTGGGTGGATGCGAGAACATATAAGAATGTTTCAACGCCACGCAACTATCTATATGAGAGTCACCTGGCAGTCTGTTCTTTCTCTGCTCAGGCTTGATGGTGATGGAATGAAAACTTCAAAGGCAGTCTTTAAAGAAAAGTACAGGGCCTTCAATGCAGCCTTTGAGGAGATATATAAGAACCAAACTGGTTGGAATGTCCCAGATCCTCAACTACGTGATGACTTGCTTATTCAAACCTCAAATTGTGTAATTCAAGCATATCGAATCCTCTGCGGGTCAAGGTGTCAATTTAACCGTGAAAAGTATATCAAATATACGACTGATGATCTGAGCAAGCATATGCTGGATCTCTTGCAAGGCTCATCGCGCTCGTTGCAAAGTTCACGCCGGAGATGA

mRNA sequence

ATGGGGGATTGTGAATCAATTATACCAACCTCTGAAGGGGAGCAACACGCTCTAGCTGCATTTCAGCACCTAGTCAAGTTCTTAAGGACAAGTCAGAACGTGAATAATGAATTGAAAAAGCTCCTTGCCGATCTCGATTCTCATTTGACTTCCTTTACGTCGTATACTCATTTAGAGGTTGGAAAGTTGAGTGAGTTAGAGACAAGGTTCAAATCTGCCGAGGAGAAGATTACGAGATGGGAATCTAACAAATCCATGATATGGGATTCTGATCCCAAAGAGGCTTGTGAATATATGAAGGCTGTTGATGAAATTCATAAAGTTCAGGAAGGTCTAAGAAGTTTATCTGCAAATGACAGCCAAAAGCAAAATGAACTTCTTTTCCAGGCCGACTCTGTCGTACAGATTGCAATGGCAAGGCTAGAGCAGGAGCTAATCCACATTCTTGCTCAGCACAAACTATATTTTGAGCCTGACTATGTTCCCTTCCAGTCTGGTGGCTACGATGTTGTCTATAGCGAGTCCTTTGTTTCAGCTGAGGACAGTTTGGAGGTTGAAACTTCCCACGGAGAGAGTATTCCTGCTTTATATGCTGTGAACTCGGTCCATCCACACGTGATCGCTCATCTTAAATCCATCGCAAATGTTATGTTTACTTCAAATTATATTCAGGAATTCTGCCTGACTTTTGTTAAAATGCGGAAAGATGCGTTGAACGAGTATCTGTTCATTCTCGAAATGGAGAAGTATAGCATTGGAGATTTGCTGACCATGGAATGGAGCGACTTAAACGTGAAGATCAAGAAATGGGTTTGGCTTATTAAAATTGTTATTAGAGTCTACCTTATTAGTGAGCAAAGACTCTGTAACAATATATTTGAAGGCATTGGATCTTATAGTGCAGTCTGCTTTACTGAGATTTCAGCGACTTCTATGCTTCGCCTGTTGAATTTTGGAGAGGCCATAGCTATGGAACCCCATCGACCGGAAAAGCTGTTTCACTTGCTAGACATGTATGAGGTCCTAAAAAATCTTCTTGGATTCGTAGATGAATTGTTTTCCGAAGAAACAGAAAAGGGTTCTTTTCTCAAATTTGAGTTCCATAATCTTTTCAAGAAATTGGGTGATTCTGCAAGGGCTACGTTTTTAGATTTTGGAAATTTCATTGCATGTTCCATATCAACAAACCCTTTTCCCGGCGGTGGTGTTCATCATCTAACAAGGTATGTAATGAACTACATCCATACCCTTACTGTTTTCAGAGACTCGCTTGTTATCCTTCTCCAAGATCAAGCAGCAGATGACTTAAGCCCAACTTCTGAGCTGCAGAGTGAAATCAATAGCATACCTTGCCCAATGGCTTACCACCTTCAGTCAATCATTTCACATTTGCTATCCAATCTCAATAACAAATCCAAGTTGTACAAGGATGGTGCTCTACGACATATTTTCTTAATGAACAACATCCATTATATAGTTCAAAAGGTTGAAAACTCTGATCTCATAGCCTTTCTTGGAAGTGGGTGGATGCGAGAACATATAAGAATGTTTCAACGCCACGCAACTATCTATATGAGAGTCACCTGGCAGTCTGTTCTTTCTCTGCTCAGGCTTGATGGTGATGGAATGAAAACTTCAAAGGCAGTCTTTAAAGAAAAGTACAGGGCCTTCAATGCAGCCTTTGAGGAGATATATAAGAACCAAACTGGTTGGAATGTCCCAGATCCTCAACTACGTGATGACTTGCTTATTCAAACCTCAAATTGTGTAATTCAAGCATATCGAATCCTCTGCGGGTCAAGGTGTCAATTTAACCGTGAAAAGTATATCAAATATACGACTGATGATCTGAGCAAGCATATGCTGGATCTCTTGCAAGGCTCATCGCGCTCGTTGCAAAGTTCACGCCGGAGATGA

Coding sequence (CDS)

ATGGGGGATTGTGAATCAATTATACCAACCTCTGAAGGGGAGCAACACGCTCTAGCTGCATTTCAGCACCTAGTCAAGTTCTTAAGGACAAGTCAGAACGTGAATAATGAATTGAAAAAGCTCCTTGCCGATCTCGATTCTCATTTGACTTCCTTTACGTCGTATACTCATTTAGAGGTTGGAAAGTTGAGTGAGTTAGAGACAAGGTTCAAATCTGCCGAGGAGAAGATTACGAGATGGGAATCTAACAAATCCATGATATGGGATTCTGATCCCAAAGAGGCTTGTGAATATATGAAGGCTGTTGATGAAATTCATAAAGTTCAGGAAGGTCTAAGAAGTTTATCTGCAAATGACAGCCAAAAGCAAAATGAACTTCTTTTCCAGGCCGACTCTGTCGTACAGATTGCAATGGCAAGGCTAGAGCAGGAGCTAATCCACATTCTTGCTCAGCACAAACTATATTTTGAGCCTGACTATGTTCCCTTCCAGTCTGGTGGCTACGATGTTGTCTATAGCGAGTCCTTTGTTTCAGCTGAGGACAGTTTGGAGGTTGAAACTTCCCACGGAGAGAGTATTCCTGCTTTATATGCTGTGAACTCGGTCCATCCACACGTGATCGCTCATCTTAAATCCATCGCAAATGTTATGTTTACTTCAAATTATATTCAGGAATTCTGCCTGACTTTTGTTAAAATGCGGAAAGATGCGTTGAACGAGTATCTGTTCATTCTCGAAATGGAGAAGTATAGCATTGGAGATTTGCTGACCATGGAATGGAGCGACTTAAACGTGAAGATCAAGAAATGGGTTTGGCTTATTAAAATTGTTATTAGAGTCTACCTTATTAGTGAGCAAAGACTCTGTAACAATATATTTGAAGGCATTGGATCTTATAGTGCAGTCTGCTTTACTGAGATTTCAGCGACTTCTATGCTTCGCCTGTTGAATTTTGGAGAGGCCATAGCTATGGAACCCCATCGACCGGAAAAGCTGTTTCACTTGCTAGACATGTATGAGGTCCTAAAAAATCTTCTTGGATTCGTAGATGAATTGTTTTCCGAAGAAACAGAAAAGGGTTCTTTTCTCAAATTTGAGTTCCATAATCTTTTCAAGAAATTGGGTGATTCTGCAAGGGCTACGTTTTTAGATTTTGGAAATTTCATTGCATGTTCCATATCAACAAACCCTTTTCCCGGCGGTGGTGTTCATCATCTAACAAGGTATGTAATGAACTACATCCATACCCTTACTGTTTTCAGAGACTCGCTTGTTATCCTTCTCCAAGATCAAGCAGCAGATGACTTAAGCCCAACTTCTGAGCTGCAGAGTGAAATCAATAGCATACCTTGCCCAATGGCTTACCACCTTCAGTCAATCATTTCACATTTGCTATCCAATCTCAATAACAAATCCAAGTTGTACAAGGATGGTGCTCTACGACATATTTTCTTAATGAACAACATCCATTATATAGTTCAAAAGGTTGAAAACTCTGATCTCATAGCCTTTCTTGGAAGTGGGTGGATGCGAGAACATATAAGAATGTTTCAACGCCACGCAACTATCTATATGAGAGTCACCTGGCAGTCTGTTCTTTCTCTGCTCAGGCTTGATGGTGATGGAATGAAAACTTCAAAGGCAGTCTTTAAAGAAAAGTACAGGGCCTTCAATGCAGCCTTTGAGGAGATATATAAGAACCAAACTGGTTGGAATGTCCCAGATCCTCAACTACGTGATGACTTGCTTATTCAAACCTCAAATTGTGTAATTCAAGCATATCGAATCCTCTGCGGGTCAAGGTGTCAATTTAACCGTGAAAAGTATATCAAATATACGACTGATGATCTGAGCAAGCATATGCTGGATCTCTTGCAAGGCTCATCGCGCTCGTTGCAAAGTTCACGCCGGAGATGA

Protein sequence

MGDCESIIPTSEGEQHALAAFQHLVKFLRTSQNVNNELKKLLADLDSHLTSFTSYTHLEVGKLSELETRFKSAEEKITRWESNKSMIWDSDPKEACEYMKAVDEIHKVQEGLRSLSANDSQKQNELLFQADSVVQIAMARLEQELIHILAQHKLYFEPDYVPFQSGGYDVVYSESFVSAEDSLEVETSHGESIPALYAVNSVHPHVIAHLKSIANVMFTSNYIQEFCLTFVKMRKDALNEYLFILEMEKYSIGDLLTMEWSDLNVKIKKWVWLIKIVIRVYLISEQRLCNNIFEGIGSYSAVCFTEISATSMLRLLNFGEAIAMEPHRPEKLFHLLDMYEVLKNLLGFVDELFSEETEKGSFLKFEFHNLFKKLGDSARATFLDFGNFIACSISTNPFPGGGVHHLTRYVMNYIHTLTVFRDSLVILLQDQAADDLSPTSELQSEINSIPCPMAYHLQSIISHLLSNLNNKSKLYKDGALRHIFLMNNIHYIVQKVENSDLIAFLGSGWMREHIRMFQRHATIYMRVTWQSVLSLLRLDGDGMKTSKAVFKEKYRAFNAAFEEIYKNQTGWNVPDPQLRDDLLIQTSNCVIQAYRILCGSRCQFNREKYIKYTTDDLSKHMLDLLQGSSRSLQSSRRR*
Homology
BLAST of Chy12G220980 vs. ExPASy Swiss-Prot
Match: Q9FNR3 (Exocyst complex component EXO70E2 OS=Arabidopsis thaliana OX=3702 GN=EXO70E2 PE=1 SV=1)

HSP 1 Score: 506.5 bits (1303), Expect = 4.3e-142
Identity = 284/645 (44.03%), Postives = 403/645 (62.48%), Query Frame = 0

Query: 1   MGDCESIIPTSEGEQHALAAFQHLVKFLRTS-QNVNNELKKLLADLDSHLTSF-TSYTHL 60
           M + +S +P S    H   A  H+VK LR S  N++  L+KLL+DL+ HL++F  + T +
Sbjct: 1   MAEFDSKVPVSGMNNHVFEACHHVVKALRASDNNLDANLRKLLSDLEMHLSTFGIADTKV 60

Query: 61  EVGKLSELETRFKSAEEKITRWESNKSMIWDSDPKEACEYMKAVDEIHKVQEGLRSLSAN 120
           E    SE++ RFK A ++I  WE+N+S ++++   EA ++ +A+ ++  V  G ++L   
Sbjct: 61  EDAGFSEIKKRFKEAVKRIRSWETNQSTMFEAGLSEADQFFQALYDVQTVLVGFKALPMK 120

Query: 121 DSQKQNELLFQADSVVQIAMARLEQELIHILAQHKLYFEPDYVPFQSGGYDVVYSESFVS 180
            +Q + ++  QA   + IAM RLE+EL  +L QHK + +PDY+   S   D+VY ESFVS
Sbjct: 121 TNQMEKDVYNQATVALDIAMLRLEKELCDVLHQHKRHVQPDYLAVSSRRKDIVYDESFVS 180

Query: 181 AEDSLEVETS---HGESIPALYAVNSVHPHVIAHLKSIANVMFTSNYIQEFCLTFVKMRK 240
            +D + VE S     E I   Y  + V P V+ H+K+IAN MF   Y Q FC  F+ +++
Sbjct: 181 LDDEVIVEASSHEDDEQISDFYNSDLVDPIVLPHIKAIANAMFACEYDQPFCEAFIGVQR 240

Query: 241 DALNEYLFILEMEKYSIGDLLTMEWSDLNVKIKKWVWLIKIVIRVYLISEQRLCNNIFEG 300
           +AL EY+  LEME++S  D+L M+W DLN  ++KW  ++KI+ +VYL SE++LC+ I   
Sbjct: 241 EALEEYMVTLEMERFSCVDVLRMDWEDLNGAMRKWTKVVKIITQVYLASEKQLCDQILGD 300

Query: 301 IGSYSAVCFTEISATSMLRLLNFGEAIAMEPHRPEKLFHLLDMYEVLKNLLGFVDELFSE 360
             S S  CF EIS  ++L LLNFGEA+ +   +PE L   L MYEV   +L  VD LF +
Sbjct: 301 FESISTACFIEISKDAILSLLNFGEAVVLRSCKPEMLERFLSMYEVSAEILVDVDNLFPD 360

Query: 361 ETEKGSFLKFEFHNLFKKLGDSARATFLDFGNFIACSISTNPFPGGGVHHLTRYVMNYIH 420
           ET  GS L+  FHNL KKL D    TFL F + IA   ST PF GGG+HHLTRYVMNY+ 
Sbjct: 361 ET--GSSLRIAFHNLSKKLADHTTTTFLKFKDAIASDESTRPFHGGGIHHLTRYVMNYLK 420

Query: 421 TLTVFRDSLVILLQDQAADDLSPTSELQSEINSIPCPMAYHLQSIISHLLSNLNNKSKLY 480
            L  + DSL  LLQ+   DD  P    +  + S   PMA HL+SI++ L S+L  K++LY
Sbjct: 421 LLPEYTDSLNSLLQNIHVDDSIPEKTGEDVLPSTFSPMARHLRSIVTTLESSLERKAQLY 480

Query: 481 KDGALRHIFLMNNIHYIVQKVENSDLIAFLGSGWMREHIRMFQRHATIYMRVTWQSVLSL 540
            D AL+ IFLMNN  Y+VQKV+ S+L    G  W+R+HI  +Q + T Y R TW S+L+L
Sbjct: 481 ADEALKSIFLMNNFRYMVQKVKGSELRRLFGDEWIRKHIASYQCNVTNYERSTWSSILAL 540

Query: 541 LRLDGDGMKTSKAVFKEKYRAFNAAFEEIYKNQTGWNVPDPQLRDDLLIQTSNCVIQAYR 600
           LR + D ++T     +E+ R F+ AF+++YKNQT W+VPD +LRDDL I TS  V+Q+YR
Sbjct: 541 LRDNNDSVRT----LRERCRLFSLAFDDVYKNQTRWSVPDSELRDDLHISTSVKVVQSYR 600

Query: 601 ILCGSRCQFNREKYIKYTTDDLSKHMLDLLQ--GSSRSLQSSRRR 639
              G       EK+I+YT +D+   +LDL +   S RSL+SSR+R
Sbjct: 601 GFLGRNAVRIGEKHIRYTCEDIENMLLDLFECLPSPRSLRSSRKR 639

BLAST of Chy12G220980 vs. ExPASy Swiss-Prot
Match: Q9FGH9 (Exocyst complex component EXO70B1 OS=Arabidopsis thaliana OX=3702 GN=EXO70B1 PE=1 SV=1)

HSP 1 Score: 255.4 bits (651), Expect = 1.7e-66
Identity = 183/643 (28.46%), Postives = 321/643 (49.92%), Query Frame = 0

Query: 13  GEQHALAAFQHLVKFLRTSQNVNNELKKLLADLDSHLTSFTSYTHLEVGKLSE------- 72
           GE+  LA  +H+ K L  ++++ +++ ++ ++ D       S   L  G+  E       
Sbjct: 5   GEEKLLAVARHIAKTLGHNESMADDILQIFSNFDGRF----SREKLAEGQAGEDGSGVAT 64

Query: 73  LETRFKSAEEKITRWESNKSMIWDSDPKEACEYMKAVDEIHKVQEGLRSLSANDSQKQNE 132
           LE    S + +I+R+ +    IW +DP ++  ++  +DE+  +   +R  S   S+K   
Sbjct: 65  LERALNSIDGQISRFVAADQPIW-ADPADSAAFLDTIDELVAI---IREWSPMASEKPIG 124

Query: 133 L-LFQADSVVQIAMARLEQELIHILAQHKLYFEPDYVPFQSGGYDVVYSESFVSAEDSLE 192
           + L +AD ++Q AM R+E+E   ++ +    F  +  P    G      +S    +D  +
Sbjct: 125 ICLTRADDMMQQAMFRIEEEFRSLMERGAESFGLN--PQGDAGAMNHRFDSEEEEDDDRD 184

Query: 193 VETSHGESIPAL-------YAVNSVHPHVIAHLKSIANVMFTSNYIQEFCLTFVKMRKDA 252
                   IP           ++++    I  L  +A  M  + + +     +   R++ 
Sbjct: 185 FNNGDDIQIPVAQPLTDYDLIIDALPSATINDLHEMAKRMLGAGFGKACSHVYSSCRREF 244

Query: 253 LNEYLFILEMEKYSIGDLLTMEWSDLNVKIKKWVWLIKIVIRVYLISEQRLCNNIFEGIG 312
           L E +  L ++K SI ++  M W +L  +I +W+    + +R+   SE+RLC+ +F G  
Sbjct: 245 LEESMSRLGLQKLSIEEVHKMPWQELEDEIDRWIKAANVALRILFPSERRLCDRVFFGFS 304

Query: 313 SYSAVCFTEISATSMLRLLNFGEAIAMEPHRPEKLFHLLDMYEVLKNLLGFVDELFSEET 372
           S + + F E+   S ++LLNF +AIA+    PE+LF +LD++E +++L+   + +FS++ 
Sbjct: 305 SAADLSFMEVCRGSTIQLLNFADAIAIGSRSPERLFKVLDVFETMRDLMPEFESVFSDQF 364

Query: 373 EKGSFLKFEFHNLFKKLGDSARATFLDFGNFIACSISTNPFPGGGVHHLTRYVMNYIHTL 432
              S L+ E   ++K+LG++ R  F++  N I    +    PGGG+H +TRYVMNY+   
Sbjct: 365 --CSVLRNEAVTIWKRLGEAIRGIFMELENLIRRDPAKAAVPGGGLHPITRYVMNYLRAA 424

Query: 433 TVFRDSLVILLQDQAADDLSPTSELQSEINSIPCP----MAYHLQSIISHLLSNLNNKSK 492
              R +L                ++  E N +P      +   +  I+  L SNL  KSK
Sbjct: 425 CRSRQTL---------------EQVFEESNGVPSKDSTLLTVQMSWIMELLESNLEVKSK 484

Query: 493 LYKDGALRHIFLMNNIHYIVQKVENSDLIAFLGSGWMREHIRMFQRHATIYMRVTWQSVL 552
           +YKD AL ++FLMNN  YIVQKV++ DL   LG  W+R+H    +++   Y R +W  +L
Sbjct: 485 VYKDPALCYVFLMNNGRYIVQKVKDGDLGLLLGDDWIRKHNVKVKQYHMNYQRSSWNKML 544

Query: 553 SLLRLDGD--GMKTSKAVFKEKYRAFNAAFEEIYKNQTGWNVPDPQLRDDLLIQTSNCVI 612
            LL++D    GM       KEK + FN  F+EI K  + W V D QL+++L I  +  ++
Sbjct: 545 GLLKVDNTAAGMNGLGKTMKEKLKQFNIQFDEICKVHSTWVVFDEQLKEELKISLARLLV 604

Query: 613 QAYRILCGSRCQF-----NREKYIKYTTDDLSKHMLDLLQGSS 630
            AY    G          N +KYIKY  +D+   + +L +G++
Sbjct: 605 PAYGSFIGRFQNLGDIGKNADKYIKYGVEDIEARINELFKGTT 620

BLAST of Chy12G220980 vs. ExPASy Swiss-Prot
Match: Q9LZD3 (Exocyst complex component EXO70A1 OS=Arabidopsis thaliana OX=3702 GN=EXO70A1 PE=1 SV=1)

HSP 1 Score: 251.1 bits (640), Expect = 3.3e-65
Identity = 184/639 (28.79%), Postives = 314/639 (49.14%), Query Frame = 0

Query: 28  LRTSQNVNNELKKLLADLDSHLTSFTS-------YTHLEVGKLSELETRFKSAEEKITRW 87
           L+ SQ + + +  +L   DS L++  +        TH        ++   K+AE  ++++
Sbjct: 21  LQKSQTITDNVVSILGSFDSRLSALETAMRPTQIRTHAIRKAHENIDRTLKAAEVILSQF 80

Query: 88  E---SNKSMIWDSDPKEACEYMKAVDEIHKVQEGLRSLSANDSQKQNE-LLFQADSVVQI 147
           +     ++ +     ++   Y+ A+ ++ K+   +R   +N S K ++ +L  A+S++  
Sbjct: 81  DLLRQAETKVLKGPHEDLESYLDAIAQLRKI---IRYFMSNKSFKSSDGVLNHANSLLAK 140

Query: 148 AMARLEQELIHILAQHKLYFEPDYVPFQSGGYDVVYSESFVSAEDSLEVETSHG-----E 207
           A ++LE+E   +LA +    EPD +      +D +   S   + D       HG     +
Sbjct: 141 AQSKLEEEFKQLLASYSKAVEPDRL------FDGL-PNSLRPSSDGDGGGKPHGGHHNDD 200

Query: 208 SIPALYAVNSVHP-HVIAHLKSIANVMFTSNYIQEFCLTFVKMRKDALNEYLFILEMEKY 267
           +  A Y +  + P  V+  L  +A  M  + + Q+    +   R   L E L  L +EK 
Sbjct: 201 AETAAYTLPILIPSRVLPLLHDLAQQMVQAGHQQQLLQIYRDTRSFVLEESLKKLGVEKL 260

Query: 268 SIGDLLTMEWSDLNVKIKKWVWLIKIVIRVYLISEQRLCNNIFEGIGSYSAVCFTEISAT 327
           S  D+  M+W  L  KI  W+  ++I +++    E+++C+ IF G  S S  CF E++ +
Sbjct: 261 SKEDVQRMQWEVLEAKIGNWIHFMRIAVKLLFAGERQVCDQIFRGFDSLSDQCFAEVTVS 320

Query: 328 SMLRLLNFGEAIAMEPHRPEKLFHLLDMYEVLKNLLGFVDELFSEETEKGSF---LKFEF 387
           S+  LL+FG+AIA     PEKLF LLDMYE+++ L   ++ +F     KG     ++   
Sbjct: 321 SVSMLLSFGDAIARSKRSPEKLFVLLDMYEIMRELHTEIETIF-----KGKACLEIRDSA 380

Query: 388 HNLFKKLGDSARATFLDFGNFIACSISTNPFPGGGVHHLTRYVMNYIHTLTVFRDSLVIL 447
             L K+L  +A+ TF DF   +    +      G VH LT YV+NY+  L  ++ +L  L
Sbjct: 381 TGLTKRLAQTAQETFGDFEEAVEKDATKTAVLDGTVHPLTSYVINYVKFLFDYQTTLKQL 440

Query: 448 LQDQAADDLSPTSELQSEINSIPCPMAYHLQSIISHLLSNLNNKSKLYKDGALRHIFLMN 507
             +    D     +  S++ S+          I+  L +NL+ KSK YKD AL H+FLMN
Sbjct: 441 FLEFGNGD-----DSNSQLASV-------TMRIMQALQNNLDGKSKQYKDPALTHLFLMN 500

Query: 508 NIHYIVQKVENSDLIAFLGSGWMREHIRMFQRHATIYMRVTWQSVLSLLRLD-------- 567
           NIHY+V+ V  S+    LG  W++ H R+ Q+HA  Y RV W  +L              
Sbjct: 501 NIHYMVRSVRRSEAKDLLGDDWVQRHRRIVQQHANQYKRVAWTKILQSSSAQGLTSSGGG 560

Query: 568 ----GDGMKTSKAVFKEKYRAFNAAFEEIYKNQTGWNVPDPQLRDDLLIQTSNCVIQAYR 627
               G+    S+ + KE+++ FN  F+E+++ Q+ W VPD +LR+ L +  +  ++ AYR
Sbjct: 561 SLEGGNSSGVSRGLLKERFKMFNMQFDELHQRQSQWTVPDTELRESLRLAVAEVLLPAYR 620

Query: 628 ILCGS-----RCQFNREKYIKYTTDDLSKHMLDLLQGSS 630
                         N +KYIKYT +DL + + +L +G S
Sbjct: 621 SFLKRFGPLVESGKNPQKYIKYTAEDLERLLGELFEGKS 632

BLAST of Chy12G220980 vs. ExPASy Swiss-Prot
Match: Q9LMJ4 (Exocyst complex component EXO70B2 OS=Arabidopsis thaliana OX=3702 GN=EXO70B2 PE=1 SV=1)

HSP 1 Score: 221.5 bits (563), Expect = 2.8e-56
Identity = 164/571 (28.72%), Postives = 281/571 (49.21%), Query Frame = 0

Query: 72  SAEEKITRWESNKSMIWDSDPKEACEYMKAVDEIHKVQEGLRSLSANDSQKQNELLFQAD 131
           S E +I+ +      IW SDP ++  ++ +VDE+  +   LRS++ + S    +   +AD
Sbjct: 66  SLERQISSYIVEDRPIW-SDPVDSRTFLDSVDELLAIAGDLRSMAGDKSVAVCQ--SRAD 125

Query: 132 SVVQIAMARLEQELIHILAQHKLYFEPDYVPFQSGGYDVVYSESFVSAEDSLEVETSHGE 191
            ++Q  M RL++E   ++ +    F+ D              + F   ED+   +TS G 
Sbjct: 126 ELIQQVMFRLQEEFGFVMDRAPDSFDSD--------------DEFPGEEDN---DTSDGV 185

Query: 192 SIPA-----LYAVNSVHPHVIAHLKSIANVMFTSNYIQEFCLTFVKMRKDALNEYLFILE 251
            +          + ++   VI  L +IA  M    + +E    +   R++ L E L  L 
Sbjct: 186 IVARPITDYKIVIEALQSSVIGDLNAIAVRMVAGGFAKECSRVYSSRRREFLEESLSRLH 245

Query: 252 MEKYSIGDLLTMEWSDLNVKIKKWVWLIKIVIRVYLISEQRLCNNIFEG--IGSYSAVCF 311
           +   S+ ++    W DL  +I +W+  + ++  V+  SE+ LC+ +F    + S + + F
Sbjct: 246 LRGLSMEEVQESPWQDLEDEIDRWIKAVTLIFHVFFPSERLLCDRVFSDLPVSSVTDLSF 305

Query: 312 TEISATSMLRLLNFGEAIAMEPHRPEKLFHLLDMYEVLKNLLGFVDELFSEETEKGSFLK 371
            E+   +  +LLNF +AIA+    PE+LF ++D+YE +++L+  ++ LFS+     S L+
Sbjct: 306 MEVCRGTTTQLLNFADAIALGSRLPERLFKVVDLYEAMQDLIPKMETLFSD--RYCSPLR 365

Query: 372 FEFHNLFKKLGDSARATFLDFGNFIACSISTNPFPGGGVHHLTRYVMNYIHTLTVFRDSL 431
            E   + K+LG++ R  F++  N I        FPGGG+H +TRYVMNY+      R SL
Sbjct: 366 HEALAIHKRLGEAIRGIFMELENLIRRDPPKTAFPGGGIHPITRYVMNYLRAACKSRQSL 425

Query: 432 VILLQDQAADDLSPTSELQSEINSIPCPMAYHLQSIISHLLSNLNNKSKLYKDGALRHIF 491
             +L DQ            +E  S   P++  +  ++  L SNL  K + Y+D +L  +F
Sbjct: 426 EQIL-DQTG----------NETGSDTRPLSVQIIWVLELLESNLEGKKRTYRDPSLCFLF 485

Query: 492 LMNNIHYIVQKVENSDLIAFLGSGWMREHIRMFQRHATIYMRVTWQSVLSLLRLDGDGMK 551
           +MNN  YI+ K ++++L   LG  W+ +H    +++ + Y R +W  V+ LLR DG   K
Sbjct: 486 MMNNDKYILDKAKDNELGLVLGEDWIVKHAAKLRQYHSNYRRSSWNQVVGLLRTDGPYPK 545

Query: 552 TSKAVFKEKYRAFNAAFEEIYKNQTGWNVPDPQLRDDLLIQTSNCVIQAY-----RILCG 611
                  E  R F + F+E+ K Q+ W V D QLR++L    +  V  AY     R+   
Sbjct: 546 -----LVENLRLFKSQFDEVCKVQSQWVVSDGQLREELRSSVAGIVSPAYSNFIRRLKES 598

Query: 612 SRCQFNR-EKYIKYTTDDLSKHMLDLLQGSS 630
                 R E +I YT +D+   +  L + SS
Sbjct: 606 PEINGRRGEPFIPYTVEDVEFIIKRLFKESS 598

BLAST of Chy12G220980 vs. ExPASy Swiss-Prot
Match: F4KG58 (Exocyst complex component EXO70A3 OS=Arabidopsis thaliana OX=3702 GN=EXO70A3 PE=1 SV=1)

HSP 1 Score: 202.6 bits (514), Expect = 1.3e-50
Identity = 130/446 (29.15%), Postives = 224/446 (50.22%), Query Frame = 0

Query: 202 VHPHVIAHLKSIANVMFTSNYIQEFCLTFVKMRKDALNEYLFILEMEKYSIGDLLTMEWS 261
           + P V+  L  +A  M  + + QE   T+  +R+  L + L  L +E++S  D+  M   
Sbjct: 156 IPPTVLPVLHDLAQQMVKAGHQQELFKTYRDIRRAVLAQSLEKLGVERHSKYDVERMNQD 215

Query: 262 DLNVKIKKWVWLIKIVIRVYLISEQRLCNNIFEGIGSYSAVCFTEISATSMLRLLNFGEA 321
               KI  W+  I+I +++   +E+ +C+ I +G+  +    F EI+  S   LL+FG A
Sbjct: 216 VFEAKIMNWIHYIRISVKLLFAAEKEICHQILDGVEPFRDQSFAEITTISFGMLLSFGYA 275

Query: 322 IAMEPHRPEKLFHLLDMYEVLKNLLGFVDELFSEETEKGSFLKFEFHNLFKKLGDSARAT 381
           IA+    PEK+F +LDMYE++  L    + +F   ++  + +K +  NL K L  + + T
Sbjct: 276 IAISRRSPEKVFVILDMYEIMIELQPEFELIFG--SKPCTEMKEDALNLTKLLAQTVKET 335

Query: 382 FLDFGNFIACSISTNPFPGGGVHHLTRYVMNYIHTLTVFRDSLVILLQDQAADDLSPTSE 441
             DF   I    +      G VH LT YV  Y+  L  +  +L  L Q+  ++D  P ++
Sbjct: 336 IADFEVAIEMDATETVVMDGSVHALTSYVARYVKFLFDYEPTLRQLFQEFNSND--PDTK 395

Query: 442 LQSEINSIPCPMAYHLQSIISHLLSNLNNKSKLYKDGALRHIFLMNNIHYIVQKVENSDL 501
           L+S            +  I+  L +NL+ KS+ ++D AL  +FLMNN++YIV+     + 
Sbjct: 396 LKSV-----------MTGIMRALRNNLDGKSRQFEDAALTQLFLMNNVYYIVRNFRREEA 455

Query: 502 IAFLGSGWMREHIRMFQRHATIYMRVTWQSVLSLLRLDGD------GMKTSKAVFKEKYR 561
             FLG   ++ H R+ Q+HA  Y  ++W  +L  + +              K + KEK++
Sbjct: 456 KNFLGDDLVQTHRRIVQQHAKQYQTISWNKILQCITVQSSKSGLIKNESIKKTLVKEKFK 515

Query: 562 AFNAAFEEIYKNQTGWNVPDPQLRDDLLIQTSNCVIQAY-----RILCGSRCQFNREKYI 621
            FN+ FEE+++ Q  W+V D +LR+ L +  +  ++ AY     R         N +KYI
Sbjct: 516 TFNSQFEELHQRQCQWSVSDVELRESLRLAIAEVLLPAYGSFLKRFGPMIESGKNSQKYI 575

Query: 622 KYTTDDLSKHMLDLLQGSSRSLQSSR 637
           ++T +DL + + D  QG +  +   R
Sbjct: 576 RFTPEDLERMLNDFFQGKNLDVSPKR 586

BLAST of Chy12G220980 vs. ExPASy TrEMBL
Match: A0A1S4DU88 (Exocyst subunit Exo70 family protein OS=Cucumis melo OX=3656 GN=LOC103491934 PE=3 SV=1)

HSP 1 Score: 1182.9 bits (3059), Expect = 0.0e+00
Identity = 595/638 (93.26%), Postives = 608/638 (95.30%), Query Frame = 0

Query: 1   MGDCESIIPTSEGEQHALAAFQHLVKFLRTSQNVNNELKKLLADLDSHLTSFTSYTHLEV 60
           MGDCESI+PTSEGEQHALAAFQHLVKFLR SQNVNNELKKLLADLDSHLTS   YTHLEV
Sbjct: 1   MGDCESIMPTSEGEQHALAAFQHLVKFLRKSQNVNNELKKLLADLDSHLTSLPLYTHLEV 60

Query: 61  GKLSELETRFKSAEEKITRWESNKSMIWDSDPKEACEYMKAVDEIHKVQEGLRSLSANDS 120
           GKLSELETRF SAEEKI RWESNKSMIWDS PKEACEYMKAVDEIHKVQEGLRSLSANDS
Sbjct: 61  GKLSELETRFISAEEKIMRWESNKSMIWDSGPKEACEYMKAVDEIHKVQEGLRSLSANDS 120

Query: 121 QKQNELLFQADSVVQIAMARLEQELIHILAQHKLYFEPDYVPFQSGGYDVVYSESFVSAE 180
           QKQNELLFQADSVVQIAMARLEQELIHIL QHKLYFEPDYVPFQSGGYDVVY+ESFVSAE
Sbjct: 121 QKQNELLFQADSVVQIAMARLEQELIHILVQHKLYFEPDYVPFQSGGYDVVYNESFVSAE 180

Query: 181 DSLEVETSHGESIPALYAVNSVHPHVIAHLKSIANVMFTSNYIQEFCLTFVKMRKDALNE 240
           DSLEVETSHGESIPA+YAVN VHPHVI H+KSI N+MFTSNYIQEFCL FVKMRKDALNE
Sbjct: 181 DSLEVETSHGESIPAIYAVNLVHPHVIPHIKSIGNLMFTSNYIQEFCLAFVKMRKDALNE 240

Query: 241 YLFILEMEKYSIGDLLTMEWSDLNVKIKKWVWLIKIVIRVYLISEQRLCNNIFEGIGSYS 300
           YLFILEMEKYSIGDLL+MEW DLNVKIKKWVWLIKIVIRVYL SEQRLCNNIFEGIGSYS
Sbjct: 241 YLFILEMEKYSIGDLLSMEWGDLNVKIKKWVWLIKIVIRVYLTSEQRLCNNIFEGIGSYS 300

Query: 301 AVCFTEISATSMLRLLNFGEAIAMEPHRPEKLFHLLDMYEVLKNLLGFVDELFSEETEKG 360
           AVCFTEISAT+MLRLLNFGEAIAMEPHRPEKLFHLLDMYEVLKNLLG++DELFSEETEKG
Sbjct: 301 AVCFTEISATAMLRLLNFGEAIAMEPHRPEKLFHLLDMYEVLKNLLGYIDELFSEETEKG 360

Query: 361 SFLKFEFHNLFKKLGDSARATFLDFGNFIACSISTNPFPGGGVHHLTRYVMNYIHTLTVF 420
            FLK EFHNLFKKLGDSARATF+DFGNFIACSISTNPFPGGGVHHLTRYVMNYI TLTVF
Sbjct: 361 CFLKVEFHNLFKKLGDSARATFVDFGNFIACSISTNPFPGGGVHHLTRYVMNYIKTLTVF 420

Query: 421 RDSLVILLQDQAADDLSPTSELQSEINSIPCPMAYHLQSIISHLLSNLNNKSKLYKDGAL 480
           RDSLV LLQDQA D LSPTSELQSEINSIPCPMAYHLQSI SHL+SNLNNKSKLYKD AL
Sbjct: 421 RDSLVFLLQDQATDGLSPTSELQSEINSIPCPMAYHLQSITSHLISNLNNKSKLYKDDAL 480

Query: 481 RHIFLMNNIHYIVQKVENSDLIAFLGSGWMREHIRMFQRHATIYMRVTWQSVLSLLRLDG 540
           RHIFLMNNIHYIVQKVENS+L+AFLGSGWMREHIRMFQ HA IYMRVTWQSVLSLLRLDG
Sbjct: 481 RHIFLMNNIHYIVQKVENSELVAFLGSGWMREHIRMFQSHAIIYMRVTWQSVLSLLRLDG 540

Query: 541 DGMKTSKAVFKEKYRAFNAAFEEIYKNQTGWNVPDPQLRDDLLIQTSNCVIQAYRILCGS 600
           DGMKT KAVFKEKYRAFNAAFEEIYKNQTGWNVPDPQLRDDLLIQTSNCVIQAYRIL  S
Sbjct: 541 DGMKTPKAVFKEKYRAFNAAFEEIYKNQTGWNVPDPQLRDDLLIQTSNCVIQAYRILYES 600

Query: 601 RCQFNREKYIKYTTDDLSKHMLDLLQGSSRSLQSSRRR 639
           R Q NREKYIKYTTDDLSKHMLDLLQGSS SLQSSRRR
Sbjct: 601 RSQSNREKYIKYTTDDLSKHMLDLLQGSSCSLQSSRRR 638

BLAST of Chy12G220980 vs. ExPASy TrEMBL
Match: A0A5D3BIJ1 (Exocyst subunit Exo70 family protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G001380 PE=3 SV=1)

HSP 1 Score: 1167.5 bits (3019), Expect = 0.0e+00
Identity = 588/631 (93.19%), Postives = 601/631 (95.25%), Query Frame = 0

Query: 8   IPTSEGEQHALAAFQHLVKFLRTSQNVNNELKKLLADLDSHLTSFTSYTHLEVGKLSELE 67
           +PTSEGEQHALAAFQHLVKFLR SQNVNNELKKLLADLDSHLTS   YTHLEVGKLSELE
Sbjct: 1   MPTSEGEQHALAAFQHLVKFLRKSQNVNNELKKLLADLDSHLTSLPLYTHLEVGKLSELE 60

Query: 68  TRFKSAEEKITRWESNKSMIWDSDPKEACEYMKAVDEIHKVQEGLRSLSANDSQKQNELL 127
           TRF SAEEKI RWESNKSMIWDS PKEACEYMKAVDEIHKVQEGLRSLSANDSQKQNELL
Sbjct: 61  TRFISAEEKIMRWESNKSMIWDSGPKEACEYMKAVDEIHKVQEGLRSLSANDSQKQNELL 120

Query: 128 FQADSVVQIAMARLEQELIHILAQHKLYFEPDYVPFQSGGYDVVYSESFVSAEDSLEVET 187
           FQADSVVQIAMARLEQELIHIL QHKLYFEPDYVPFQSGGYDVVY+ESFVSAEDSLEVET
Sbjct: 121 FQADSVVQIAMARLEQELIHILVQHKLYFEPDYVPFQSGGYDVVYNESFVSAEDSLEVET 180

Query: 188 SHGESIPALYAVNSVHPHVIAHLKSIANVMFTSNYIQEFCLTFVKMRKDALNEYLFILEM 247
           SHGESIPA+YAVN VHPHVI H+KSI N+MFTSNYIQEFCL FVKMRKDALNEYLFILEM
Sbjct: 181 SHGESIPAIYAVNLVHPHVIPHIKSIGNLMFTSNYIQEFCLAFVKMRKDALNEYLFILEM 240

Query: 248 EKYSIGDLLTMEWSDLNVKIKKWVWLIKIVIRVYLISEQRLCNNIFEGIGSYSAVCFTEI 307
           EKYSIGDLL+MEW DLNVKIKKWVWLIKIVIRVYL SEQRLCNNIFEGIGSYSAVCFTEI
Sbjct: 241 EKYSIGDLLSMEWGDLNVKIKKWVWLIKIVIRVYLTSEQRLCNNIFEGIGSYSAVCFTEI 300

Query: 308 SATSMLRLLNFGEAIAMEPHRPEKLFHLLDMYEVLKNLLGFVDELFSEETEKGSFLKFEF 367
           SAT+MLRLLNFGEAIAMEPHRPEKLFHLLDMYEVLKNLLG++DELFSEETEKG FLK EF
Sbjct: 301 SATAMLRLLNFGEAIAMEPHRPEKLFHLLDMYEVLKNLLGYIDELFSEETEKGCFLKVEF 360

Query: 368 HNLFKKLGDSARATFLDFGNFIACSISTNPFPGGGVHHLTRYVMNYIHTLTVFRDSLVIL 427
           HNLFKKLGDSARATF+DFGNFIACSISTNPFPGGGVHHLTRYVMNYI TLTVFRDSLV L
Sbjct: 361 HNLFKKLGDSARATFVDFGNFIACSISTNPFPGGGVHHLTRYVMNYIKTLTVFRDSLVFL 420

Query: 428 LQDQAADDLSPTSELQSEINSIPCPMAYHLQSIISHLLSNLNNKSKLYKDGALRHIFLMN 487
           LQDQA D LSPTSELQSEINSIPCPMAYHLQSI SHL+SNLNNKSKLYKD ALRHIFLMN
Sbjct: 421 LQDQATDGLSPTSELQSEINSIPCPMAYHLQSITSHLISNLNNKSKLYKDDALRHIFLMN 480

Query: 488 NIHYIVQKVENSDLIAFLGSGWMREHIRMFQRHATIYMRVTWQSVLSLLRLDGDGMKTSK 547
           NIHYIVQKVENS+L+AFLGSGWMREHIRMFQ HA IYMRVTWQSVLSLLRLDGDGMKT K
Sbjct: 481 NIHYIVQKVENSELVAFLGSGWMREHIRMFQSHAIIYMRVTWQSVLSLLRLDGDGMKTPK 540

Query: 548 AVFKEKYRAFNAAFEEIYKNQTGWNVPDPQLRDDLLIQTSNCVIQAYRILCGSRCQFNRE 607
           AVFKEKYRAFNAAFEEIYKNQTGWNVPDPQLRDDLLIQTSNCVIQAYRIL  SR Q NRE
Sbjct: 541 AVFKEKYRAFNAAFEEIYKNQTGWNVPDPQLRDDLLIQTSNCVIQAYRILYESRSQSNRE 600

Query: 608 KYIKYTTDDLSKHMLDLLQGSSRSLQSSRRR 639
           KYIKYTTDDLSKHMLDLLQGSS SLQSSRRR
Sbjct: 601 KYIKYTTDDLSKHMLDLLQGSSCSLQSSRRR 631

BLAST of Chy12G220980 vs. ExPASy TrEMBL
Match: A0A6J1E6T2 (Exocyst subunit Exo70 family protein OS=Cucurbita moschata OX=3662 GN=LOC111431322 PE=3 SV=1)

HSP 1 Score: 996.1 bits (2574), Expect = 6.6e-287
Identity = 502/638 (78.68%), Postives = 548/638 (85.89%), Query Frame = 0

Query: 1   MGDCESIIPTSEGEQHALAAFQHLVKFLRTSQNVNNELKKLLADLDSHLTSFTSYTHLEV 60
           MGDCESIIPTSEGEQH +AAFQHL K LRTSQNVN+ELK+LL DLDSHLTS T YTHL+ 
Sbjct: 1   MGDCESIIPTSEGEQHVIAAFQHLAKLLRTSQNVNDELKRLLIDLDSHLTSMTLYTHLQD 60

Query: 61  GKLSELETRFKSAEEKITRWESNKSMIWDSDPKEACEYMKAVDEIHKVQEGLRSLSANDS 120
           GKL+ELE+RFKSAEEKI RWESN+SMIWDS PKEACEYMKAVDEIHKVQEGL SL  ND 
Sbjct: 61  GKLNELESRFKSAEEKIRRWESNESMIWDSGPKEACEYMKAVDEIHKVQEGLISLPVNDG 120

Query: 121 QKQNELLFQADSVVQIAMARLEQELIHILAQHKLYFEPDYVPFQSGGYDVVYSESFVSAE 180
           +KQ +LL QA+SV+Q AM+RLEQEL+HILAQHKLYFEP+ VPFQSGGYDV+Y+ESF S E
Sbjct: 121 RKQKDLLSQAESVIQFAMSRLEQELVHILAQHKLYFEPNCVPFQSGGYDVLYNESFASLE 180

Query: 181 DSLEVETSHGESIPALYAVNSVHPHVIAHLKSIANVMFTSNYIQEFCLTFVKMRKDALNE 240
           DSLE ETS  ESIPA+  VN VHPHVI HLKSIAN MFTSNYIQEF L FV+MRKDAL+E
Sbjct: 181 DSLEEETSQRESIPAVCIVNMVHPHVIPHLKSIANAMFTSNYIQEFSLAFVRMRKDALDE 240

Query: 241 YLFILEMEKYSIGDLLTMEWSDLNVKIKKWVWLIKIVIRVYLISEQRLCNNIFEGIGSYS 300
           YL ILEME++SIGDL TMEW  LNVKIKKW+WLIK+VIRVYL SEQRLCNNIFEG GSYS
Sbjct: 241 YLLILEMERHSIGDLQTMEWDVLNVKIKKWIWLIKVVIRVYLASEQRLCNNIFEGFGSYS 300

Query: 301 AVCFTEISATSMLRLLNFGEAIAMEPHRPEKLFHLLDMYEVLKNLLGFVDELFSEETEKG 360
           A CF EISATSML LLNFGEAIAM   RPEKLF  LDMYEVL+NLLG +  LFSE+TE G
Sbjct: 301 ATCFAEISATSMLCLLNFGEAIAMTTRRPEKLFRFLDMYEVLENLLGDIGALFSEDTEMG 360

Query: 361 SFLKFEFHNLFKKLGDSARATFLDFGNFIACSISTNPFPGGGVHHLTRYVMNYIHTLTVF 420
           SFLK +FHNLF KLGDSARATF+DFG+ IA SIST+PFPGGGVHHLT+YVMNYI TLTV+
Sbjct: 361 SFLKADFHNLFMKLGDSARATFVDFGSLIARSISTSPFPGGGVHHLTKYVMNYIRTLTVY 420

Query: 421 RDSLVILLQDQAADDLSPTSELQSEINSIPCPMAYHLQSIISHLLSNLNNKSKLYKDGAL 480
           RDSLV LL+D+AAD L P SEL +EINS+PCP+A+HLQSI S L+SNLNNKSKLY D AL
Sbjct: 421 RDSLVFLLRDEAADGLGPASELLNEINSMPCPLAFHLQSITSQLISNLNNKSKLYNDDAL 480

Query: 481 RHIFLMNNIHYIVQKVENSDLIAFLGSGWMREHIRMFQRHATIYMRVTWQSVLSLLRLDG 540
           RHIFLMNNIHY+VQKV+ S+L AF G  W+REH RM Q HA IY RVTWQSV+SLLRLDG
Sbjct: 481 RHIFLMNNIHYMVQKVKGSELRAFFGGEWIREHNRMLQIHANIYTRVTWQSVISLLRLDG 540

Query: 541 DGMKTSKAVFKEKYRAFNAAFEEIYKNQTGWNVPDPQLRDDLLIQTSNCVIQAYRILCGS 600
           DG  T KAVFKEKYRAFN AFEEIYK QTGWNV DP LRDDL I TSNCVIQAYR L   
Sbjct: 541 DGTNTPKAVFKEKYRAFNVAFEEIYKTQTGWNVSDPDLRDDLHILTSNCVIQAYRFLRNL 600

Query: 601 RCQFNREKYIKYTTDDLSKHMLDLLQGSSRSLQSSRRR 639
           + QFN EKYIKYT DDLSKH+LDL QGS R LQSSRRR
Sbjct: 601 KSQFNCEKYIKYTIDDLSKHILDLFQGSPRLLQSSRRR 638

BLAST of Chy12G220980 vs. ExPASy TrEMBL
Match: A0A6J1KLM6 (Exocyst subunit Exo70 family protein OS=Cucurbita maxima OX=3661 GN=LOC111495300 PE=3 SV=1)

HSP 1 Score: 994.2 bits (2569), Expect = 2.5e-286
Identity = 501/638 (78.53%), Postives = 547/638 (85.74%), Query Frame = 0

Query: 1   MGDCESIIPTSEGEQHALAAFQHLVKFLRTSQNVNNELKKLLADLDSHLTSFTSYTHLEV 60
           MGDCESIIPTSEGEQH +AAFQHL K LRTSQNVN+ELK+LL DLDSHLTS T YTHL+ 
Sbjct: 1   MGDCESIIPTSEGEQHVIAAFQHLAKLLRTSQNVNDELKRLLTDLDSHLTSMTLYTHLQD 60

Query: 61  GKLSELETRFKSAEEKITRWESNKSMIWDSDPKEACEYMKAVDEIHKVQEGLRSLSANDS 120
           GKL+ELE+RFKSAEEKI RWESN+SMIWDS PKEACEYMKAVDEIHKVQEGL SL  ND 
Sbjct: 61  GKLNELESRFKSAEEKIRRWESNESMIWDSGPKEACEYMKAVDEIHKVQEGLISLPVNDG 120

Query: 121 QKQNELLFQADSVVQIAMARLEQELIHILAQHKLYFEPDYVPFQSGGYDVVYSESFVSAE 180
           +KQ +LL QA+SV+Q AMARLEQEL+HILAQ+KLYFEP+ VPFQSGGYD VY+ESF S E
Sbjct: 121 RKQKDLLCQAESVIQFAMARLEQELVHILAQNKLYFEPNCVPFQSGGYDAVYNESFASVE 180

Query: 181 DSLEVETSHGESIPALYAVNSVHPHVIAHLKSIANVMFTSNYIQEFCLTFVKMRKDALNE 240
           DSLE ETS  ESIPA+  VN VHPHVI HLKSIAN MFTSNYIQEF L FV MRKDAL+E
Sbjct: 181 DSLEEETSQRESIPAVCIVNMVHPHVIPHLKSIANAMFTSNYIQEFSLAFVIMRKDALDE 240

Query: 241 YLFILEMEKYSIGDLLTMEWSDLNVKIKKWVWLIKIVIRVYLISEQRLCNNIFEGIGSYS 300
           YL ILEME++SIGDL TMEW  LNVKIKKW+WLIK+VIRVYL SEQRLCNNIFEG GSYS
Sbjct: 241 YLLILEMERHSIGDLQTMEWDVLNVKIKKWIWLIKVVIRVYLASEQRLCNNIFEGFGSYS 300

Query: 301 AVCFTEISATSMLRLLNFGEAIAMEPHRPEKLFHLLDMYEVLKNLLGFVDELFSEETEKG 360
           A CF EISATSML LLNFGEAIAM   RPEKLF LLDMYEVL+NLL  +  LFSE+TE G
Sbjct: 301 ATCFAEISATSMLCLLNFGEAIAMTTRRPEKLFRLLDMYEVLENLLVDIGALFSEDTEMG 360

Query: 361 SFLKFEFHNLFKKLGDSARATFLDFGNFIACSISTNPFPGGGVHHLTRYVMNYIHTLTVF 420
           SFLK EFHNLF KL DSARATF+DFG+ IA SIST+PFPGGGVHHLT+YVMNY  TLTV+
Sbjct: 361 SFLKAEFHNLFMKLSDSARATFVDFGSLIARSISTSPFPGGGVHHLTKYVMNYTRTLTVY 420

Query: 421 RDSLVILLQDQAADDLSPTSELQSEINSIPCPMAYHLQSIISHLLSNLNNKSKLYKDGAL 480
           RDSLV LL+D+AAD L P SEL +EINS+PCP+A+HLQSI S L+SNLNNKSKLY D AL
Sbjct: 421 RDSLVFLLRDEAADSLGPASELLNEINSMPCPLAFHLQSITSQLISNLNNKSKLYNDDAL 480

Query: 481 RHIFLMNNIHYIVQKVENSDLIAFLGSGWMREHIRMFQRHATIYMRVTWQSVLSLLRLDG 540
           RHIFLMNNIHY++QKV+ S+L AF G  W+REH RM Q HA IYMRVTWQSV+SLLRLDG
Sbjct: 481 RHIFLMNNIHYMIQKVKGSELRAFFGGEWIREHNRMLQSHANIYMRVTWQSVISLLRLDG 540

Query: 541 DGMKTSKAVFKEKYRAFNAAFEEIYKNQTGWNVPDPQLRDDLLIQTSNCVIQAYRILCGS 600
           D   T KAVFKEKYRAFN AFEEIYKNQTGWNV DP+LRDDL I TSNCVIQAYR LC  
Sbjct: 541 DETNTPKAVFKEKYRAFNVAFEEIYKNQTGWNVSDPELRDDLHILTSNCVIQAYRFLCNL 600

Query: 601 RCQFNREKYIKYTTDDLSKHMLDLLQGSSRSLQSSRRR 639
           + QFN E+YIKYT DDLSKH+LDL QGS  SLQSSRRR
Sbjct: 601 KSQFNCERYIKYTIDDLSKHILDLFQGSPDSLQSSRRR 638

BLAST of Chy12G220980 vs. ExPASy TrEMBL
Match: A0A6J1C7N5 (Exocyst subunit Exo70 family protein OS=Momordica charantia OX=3673 GN=LOC111008738 PE=3 SV=1)

HSP 1 Score: 991.1 bits (2561), Expect = 2.1e-285
Identity = 494/639 (77.31%), Postives = 550/639 (86.07%), Query Frame = 0

Query: 1   MGDCESIIPTSEGEQHALAAFQHLVKFLRTSQNVNNELKKLLADLDSHLTSFTSYTHLEV 60
           MGDCESI+P SEGE H +AAFQHLV+ L  SQNVN+E+K+LL DLDSHLTS T YTHLE+
Sbjct: 12  MGDCESILPASEGEHHVIAAFQHLVRVLGASQNVNDEVKRLLNDLDSHLTSMTQYTHLEI 71

Query: 61  GKLSELETRFKSAEEKITRWESNKSMIWDSDPKEACEYMKAVDEIHKVQEGLRSLSANDS 120
           GKLSELE +FKSAEEKI RWESNKS+IWDS P EACEYMKAV EIHKVQEGLRSL  NDS
Sbjct: 72  GKLSELEKKFKSAEEKIRRWESNKSIIWDSGPMEACEYMKAVGEIHKVQEGLRSLPVNDS 131

Query: 121 QKQNELLFQADSVVQIAMARLEQELIHILAQHKLYFEPDYVPFQSGGYDVVYSESFVSAE 180
           +KQ ELLFQADSV+QIAMARLEQEL+HILAQHKLYFEPDY+PFQSGGYD VY+ESF SAE
Sbjct: 132 RKQKELLFQADSVMQIAMARLEQELVHILAQHKLYFEPDYIPFQSGGYDFVYNESFASAE 191

Query: 181 DSLEVETSHGESIPALYAVNSVHPHVIAHLKSIANVMFTSNYIQEFCLTFVKMRKDALNE 240
           D+L  ETS GES+ A+Y VN VHPHVI HLKSIANVMFTSNYIQEFC  FV  R+DAL+E
Sbjct: 192 DNLGEETSQGESVAAVYVVNLVHPHVIPHLKSIANVMFTSNYIQEFCKAFVNTRRDALDE 251

Query: 241 YLFILEMEKYSIGDLLTMEWSDLNVKIKKWVWLIKIVIRVYLISEQRLCNNIFEGIGSYS 300
           YLF+L+MEKYSIGDLLTM W  LNVKIK+WV +I+I+IRVYL SE+RLCNNIFEGIGSYS
Sbjct: 252 YLFVLQMEKYSIGDLLTMNWDILNVKIKRWVCVIQIIIRVYLASERRLCNNIFEGIGSYS 311

Query: 301 AVCFTEISATSMLRLLNFGEAIAMEPHRPEKLFHLLDMYEVLKNLLGFVDELFSEETEKG 360
           + CFT+ISATSM RLLNFGEAIAM P RPEKLF LLDMYEVL+NLL   D LF E++E G
Sbjct: 312 SACFTDISATSMFRLLNFGEAIAMTPRRPEKLFRLLDMYEVLENLLVDKDALFPEDSETG 371

Query: 361 SFLKFEFHNLFKKLGDSARATFLDFGNFIACSISTNPFPGGGVHHLTRYVMNYIHTLTVF 420
           SFLK EFHNLFKKLGDSARATF+DFG  I+ SIST+PFPGGGVHHLT+YVMNYI TLTV+
Sbjct: 372 SFLKSEFHNLFKKLGDSARATFVDFGYLISSSISTDPFPGGGVHHLTKYVMNYIKTLTVY 431

Query: 421 RDSLVILLQDQAADDLSPTSELQSEINSIPCPMAYHLQSIISHLLSNLNNKSKLYK-DGA 480
           RDSL+ LLQD+ AD+LSP SEL +E N++ CPMAYHLQSI SHL+SNLNNKSKLY+ D A
Sbjct: 432 RDSLIFLLQDKVADNLSPDSELHNE-NNVHCPMAYHLQSIASHLISNLNNKSKLYRDDAA 491

Query: 481 LRHIFLMNNIHYIVQKVENSDLIAFLGSGWMREHIRMFQRHATIYMRVTWQSVLSLLRLD 540
           LRH+FLMNNIHY+VQKV   +L  + G+ WMREH + FQ HAT+Y+RVTWQSVLS+L+ D
Sbjct: 492 LRHVFLMNNIHYVVQKVGGPELRLYFGNKWMREHTKTFQNHATLYIRVTWQSVLSVLKHD 551

Query: 541 GDGMKTSKAVFKEKYRAFNAAFEEIYKNQTGWNVPDPQLRDDLLIQTSNCVIQAYRILCG 600
           G G   SKA FKEKYRAF  AFEEIYKNQTGWNVPDP LRDDL IQTSNCVIQAYR LC 
Sbjct: 552 GKGTNISKADFKEKYRAFTVAFEEIYKNQTGWNVPDPDLRDDLHIQTSNCVIQAYRTLCE 611

Query: 601 SRCQFNREKYIKYTTDDLSKHMLDLLQGSSRSLQSSRRR 639
           +R  FNREKYIKYT DDL KH+LDL Q S RSLQSSRRR
Sbjct: 612 TRSPFNREKYIKYTPDDLGKHLLDLFQDSPRSLQSSRRR 649

BLAST of Chy12G220980 vs. NCBI nr
Match: XP_004137600.1 (exocyst complex component EXO70E2 [Cucumis sativus] >XP_031744501.1 exocyst complex component EXO70E2 [Cucumis sativus] >XP_031744503.1 exocyst complex component EXO70E2 [Cucumis sativus] >XP_031744509.1 exocyst complex component EXO70E2 [Cucumis sativus] >KGN64059.2 hypothetical protein Csa_014168 [Cucumis sativus])

HSP 1 Score: 1212 bits (3135), Expect = 0.0
Identity = 613/638 (96.08%), Postives = 619/638 (97.02%), Query Frame = 0

Query: 1   MGDCESIIPTSEGEQHALAAFQHLVKFLRTSQNVNNELKKLLADLDSHLTSFTSYTHLEV 60
           MGDCESIIPTSEGEQHALAAFQHLVKFLRTSQNVNNELKKLLADLDSHLTS TSYT   V
Sbjct: 1   MGDCESIIPTSEGEQHALAAFQHLVKFLRTSQNVNNELKKLLADLDSHLTSLTSYT---V 60

Query: 61  GKLSELETRFKSAEEKITRWESNKSMIWDSDPKEACEYMKAVDEIHKVQEGLRSLSANDS 120
           GKLSELETRFKSA+EKITRWESNKSMIWDS PKEA EYMKAVDEIHKVQEGLRSLSANDS
Sbjct: 61  GKLSELETRFKSAKEKITRWESNKSMIWDSGPKEASEYMKAVDEIHKVQEGLRSLSANDS 120

Query: 121 QKQNELLFQADSVVQIAMARLEQELIHILAQHKLYFEPDYVPFQSGGYDVVYSESFVSAE 180
           QKQNELLFQADSVVQIAMARLEQELIHILAQHKLYFEPDYVPFQSGGYDVVY+ESFVSAE
Sbjct: 121 QKQNELLFQADSVVQIAMARLEQELIHILAQHKLYFEPDYVPFQSGGYDVVYNESFVSAE 180

Query: 181 DSLEVETSHGESIPALYAVNSVHPHVIAHLKSIANVMFTSNYIQEFCLTFVKMRKDALNE 240
           DSLEVETSHGESIPALYAVN VHPHVIAHLKSIANVMF SNYIQEFCL FV+MRKDALNE
Sbjct: 181 DSLEVETSHGESIPALYAVNLVHPHVIAHLKSIANVMFNSNYIQEFCLAFVRMRKDALNE 240

Query: 241 YLFILEMEKYSIGDLLTMEWSDLNVKIKKWVWLIKIVIRVYLISEQRLCNNIFEGIGSYS 300
           YLFILEMEK+SIGDLLTMEWSDLNVKIKKWVWLIKIVIRVYL SEQRLCNNIFEGIGSYS
Sbjct: 241 YLFILEMEKFSIGDLLTMEWSDLNVKIKKWVWLIKIVIRVYLTSEQRLCNNIFEGIGSYS 300

Query: 301 AVCFTEISATSMLRLLNFGEAIAMEPHRPEKLFHLLDMYEVLKNLLGFVDELFSEETEKG 360
           AVCFTEISATSMLRLLNFGEAIAMEPHRPEKLFHLLDMYEVLKNLLGFVDELFSEETEKG
Sbjct: 301 AVCFTEISATSMLRLLNFGEAIAMEPHRPEKLFHLLDMYEVLKNLLGFVDELFSEETEKG 360

Query: 361 SFLKFEFHNLFKKLGDSARATFLDFGNFIACSISTNPFPGGGVHHLTRYVMNYIHTLTVF 420
           SFLKFEFHNLFKKLG SARATFLDFGNFIACSISTNPFPGGGVHHLTRYVMNYIHTLTVF
Sbjct: 361 SFLKFEFHNLFKKLGVSARATFLDFGNFIACSISTNPFPGGGVHHLTRYVMNYIHTLTVF 420

Query: 421 RDSLVILLQDQAADDLSPTSELQSEINSIPCPMAYHLQSIISHLLSNLNNKSKLYKDGAL 480
           RDSLV LLQDQA D LSPT+ELQSE+NSIPCPMAYHLQSI SHLLSNLNNKSKLYKD AL
Sbjct: 421 RDSLVFLLQDQATDVLSPTTELQSEVNSIPCPMAYHLQSITSHLLSNLNNKSKLYKDDAL 480

Query: 481 RHIFLMNNIHYIVQKVENSDLIAFLGSGWMREHIRMFQRHATIYMRVTWQSVLSLLRLDG 540
           RHIFLMNNIHYIVQKVENSDLIAFLGSGWMREHIRMFQ HATIYMR TWQSVLSLLRLDG
Sbjct: 481 RHIFLMNNIHYIVQKVENSDLIAFLGSGWMREHIRMFQSHATIYMRATWQSVLSLLRLDG 540

Query: 541 DGMKTSKAVFKEKYRAFNAAFEEIYKNQTGWNVPDPQLRDDLLIQTSNCVIQAYRILCGS 600
           DGMKTSKAVFKEKYRAFNAAFEEIYKNQTGWNVPDPQLRDDLLIQTSNCVIQAYRILCGS
Sbjct: 541 DGMKTSKAVFKEKYRAFNAAFEEIYKNQTGWNVPDPQLRDDLLIQTSNCVIQAYRILCGS 600

Query: 601 RCQFNREKYIKYTTDDLSKHMLDLLQGSSRSLQSSRRR 638
           R QFNREKYIKYTTDDLSKHMLDLLQGSSRSLQSSRRR
Sbjct: 601 RSQFNREKYIKYTTDDLSKHMLDLLQGSSRSLQSSRRR 635

BLAST of Chy12G220980 vs. NCBI nr
Match: XP_008450312.1 (PREDICTED: exocyst complex component EXO70A1 [Cucumis melo] >XP_008450459.1 PREDICTED: exocyst complex component EXO70A1 [Cucumis melo] >XP_016899546.1 PREDICTED: exocyst complex component EXO70A1 [Cucumis melo] >XP_016899547.1 PREDICTED: exocyst complex component EXO70A1 [Cucumis melo] >XP_016899548.1 PREDICTED: exocyst complex component EXO70A1 [Cucumis melo])

HSP 1 Score: 1184 bits (3064), Expect = 0.0
Identity = 595/638 (93.26%), Postives = 608/638 (95.30%), Query Frame = 0

Query: 1   MGDCESIIPTSEGEQHALAAFQHLVKFLRTSQNVNNELKKLLADLDSHLTSFTSYTHLEV 60
           MGDCESI+PTSEGEQHALAAFQHLVKFLR SQNVNNELKKLLADLDSHLTS   YTHLEV
Sbjct: 1   MGDCESIMPTSEGEQHALAAFQHLVKFLRKSQNVNNELKKLLADLDSHLTSLPLYTHLEV 60

Query: 61  GKLSELETRFKSAEEKITRWESNKSMIWDSDPKEACEYMKAVDEIHKVQEGLRSLSANDS 120
           GKLSELETRF SAEEKI RWESNKSMIWDS PKEACEYMKAVDEIHKVQEGLRSLSANDS
Sbjct: 61  GKLSELETRFISAEEKIMRWESNKSMIWDSGPKEACEYMKAVDEIHKVQEGLRSLSANDS 120

Query: 121 QKQNELLFQADSVVQIAMARLEQELIHILAQHKLYFEPDYVPFQSGGYDVVYSESFVSAE 180
           QKQNELLFQADSVVQIAMARLEQELIHIL QHKLYFEPDYVPFQSGGYDVVY+ESFVSAE
Sbjct: 121 QKQNELLFQADSVVQIAMARLEQELIHILVQHKLYFEPDYVPFQSGGYDVVYNESFVSAE 180

Query: 181 DSLEVETSHGESIPALYAVNSVHPHVIAHLKSIANVMFTSNYIQEFCLTFVKMRKDALNE 240
           DSLEVETSHGESIPA+YAVN VHPHVI H+KSI N+MFTSNYIQEFCL FVKMRKDALNE
Sbjct: 181 DSLEVETSHGESIPAIYAVNLVHPHVIPHIKSIGNLMFTSNYIQEFCLAFVKMRKDALNE 240

Query: 241 YLFILEMEKYSIGDLLTMEWSDLNVKIKKWVWLIKIVIRVYLISEQRLCNNIFEGIGSYS 300
           YLFILEMEKYSIGDLL+MEW DLNVKIKKWVWLIKIVIRVYL SEQRLCNNIFEGIGSYS
Sbjct: 241 YLFILEMEKYSIGDLLSMEWGDLNVKIKKWVWLIKIVIRVYLTSEQRLCNNIFEGIGSYS 300

Query: 301 AVCFTEISATSMLRLLNFGEAIAMEPHRPEKLFHLLDMYEVLKNLLGFVDELFSEETEKG 360
           AVCFTEISAT+MLRLLNFGEAIAMEPHRPEKLFHLLDMYEVLKNLLG++DELFSEETEKG
Sbjct: 301 AVCFTEISATAMLRLLNFGEAIAMEPHRPEKLFHLLDMYEVLKNLLGYIDELFSEETEKG 360

Query: 361 SFLKFEFHNLFKKLGDSARATFLDFGNFIACSISTNPFPGGGVHHLTRYVMNYIHTLTVF 420
            FLK EFHNLFKKLGDSARATF+DFGNFIACSISTNPFPGGGVHHLTRYVMNYI TLTVF
Sbjct: 361 CFLKVEFHNLFKKLGDSARATFVDFGNFIACSISTNPFPGGGVHHLTRYVMNYIKTLTVF 420

Query: 421 RDSLVILLQDQAADDLSPTSELQSEINSIPCPMAYHLQSIISHLLSNLNNKSKLYKDGAL 480
           RDSLV LLQDQA D LSPTSELQSEINSIPCPMAYHLQSI SHL+SNLNNKSKLYKD AL
Sbjct: 421 RDSLVFLLQDQATDGLSPTSELQSEINSIPCPMAYHLQSITSHLISNLNNKSKLYKDDAL 480

Query: 481 RHIFLMNNIHYIVQKVENSDLIAFLGSGWMREHIRMFQRHATIYMRVTWQSVLSLLRLDG 540
           RHIFLMNNIHYIVQKVENS+L+AFLGSGWMREHIRMFQ HA IYMRVTWQSVLSLLRLDG
Sbjct: 481 RHIFLMNNIHYIVQKVENSELVAFLGSGWMREHIRMFQSHAIIYMRVTWQSVLSLLRLDG 540

Query: 541 DGMKTSKAVFKEKYRAFNAAFEEIYKNQTGWNVPDPQLRDDLLIQTSNCVIQAYRILCGS 600
           DGMKT KAVFKEKYRAFNAAFEEIYKNQTGWNVPDPQLRDDLLIQTSNCVIQAYRIL  S
Sbjct: 541 DGMKTPKAVFKEKYRAFNAAFEEIYKNQTGWNVPDPQLRDDLLIQTSNCVIQAYRILYES 600

Query: 601 RCQFNREKYIKYTTDDLSKHMLDLLQGSSRSLQSSRRR 638
           R Q NREKYIKYTTDDLSKHMLDLLQGSS SLQSSRRR
Sbjct: 601 RSQSNREKYIKYTTDDLSKHMLDLLQGSSCSLQSSRRR 638

BLAST of Chy12G220980 vs. NCBI nr
Match: TYJ98937.1 (exocyst complex component EXO70A1 [Cucumis melo var. makuwa])

HSP 1 Score: 1169 bits (3025), Expect = 0.0
Identity = 588/631 (93.19%), Postives = 601/631 (95.25%), Query Frame = 0

Query: 8   IPTSEGEQHALAAFQHLVKFLRTSQNVNNELKKLLADLDSHLTSFTSYTHLEVGKLSELE 67
           +PTSEGEQHALAAFQHLVKFLR SQNVNNELKKLLADLDSHLTS   YTHLEVGKLSELE
Sbjct: 1   MPTSEGEQHALAAFQHLVKFLRKSQNVNNELKKLLADLDSHLTSLPLYTHLEVGKLSELE 60

Query: 68  TRFKSAEEKITRWESNKSMIWDSDPKEACEYMKAVDEIHKVQEGLRSLSANDSQKQNELL 127
           TRF SAEEKI RWESNKSMIWDS PKEACEYMKAVDEIHKVQEGLRSLSANDSQKQNELL
Sbjct: 61  TRFISAEEKIMRWESNKSMIWDSGPKEACEYMKAVDEIHKVQEGLRSLSANDSQKQNELL 120

Query: 128 FQADSVVQIAMARLEQELIHILAQHKLYFEPDYVPFQSGGYDVVYSESFVSAEDSLEVET 187
           FQADSVVQIAMARLEQELIHIL QHKLYFEPDYVPFQSGGYDVVY+ESFVSAEDSLEVET
Sbjct: 121 FQADSVVQIAMARLEQELIHILVQHKLYFEPDYVPFQSGGYDVVYNESFVSAEDSLEVET 180

Query: 188 SHGESIPALYAVNSVHPHVIAHLKSIANVMFTSNYIQEFCLTFVKMRKDALNEYLFILEM 247
           SHGESIPA+YAVN VHPHVI H+KSI N+MFTSNYIQEFCL FVKMRKDALNEYLFILEM
Sbjct: 181 SHGESIPAIYAVNLVHPHVIPHIKSIGNLMFTSNYIQEFCLAFVKMRKDALNEYLFILEM 240

Query: 248 EKYSIGDLLTMEWSDLNVKIKKWVWLIKIVIRVYLISEQRLCNNIFEGIGSYSAVCFTEI 307
           EKYSIGDLL+MEW DLNVKIKKWVWLIKIVIRVYL SEQRLCNNIFEGIGSYSAVCFTEI
Sbjct: 241 EKYSIGDLLSMEWGDLNVKIKKWVWLIKIVIRVYLTSEQRLCNNIFEGIGSYSAVCFTEI 300

Query: 308 SATSMLRLLNFGEAIAMEPHRPEKLFHLLDMYEVLKNLLGFVDELFSEETEKGSFLKFEF 367
           SAT+MLRLLNFGEAIAMEPHRPEKLFHLLDMYEVLKNLLG++DELFSEETEKG FLK EF
Sbjct: 301 SATAMLRLLNFGEAIAMEPHRPEKLFHLLDMYEVLKNLLGYIDELFSEETEKGCFLKVEF 360

Query: 368 HNLFKKLGDSARATFLDFGNFIACSISTNPFPGGGVHHLTRYVMNYIHTLTVFRDSLVIL 427
           HNLFKKLGDSARATF+DFGNFIACSISTNPFPGGGVHHLTRYVMNYI TLTVFRDSLV L
Sbjct: 361 HNLFKKLGDSARATFVDFGNFIACSISTNPFPGGGVHHLTRYVMNYIKTLTVFRDSLVFL 420

Query: 428 LQDQAADDLSPTSELQSEINSIPCPMAYHLQSIISHLLSNLNNKSKLYKDGALRHIFLMN 487
           LQDQA D LSPTSELQSEINSIPCPMAYHLQSI SHL+SNLNNKSKLYKD ALRHIFLMN
Sbjct: 421 LQDQATDGLSPTSELQSEINSIPCPMAYHLQSITSHLISNLNNKSKLYKDDALRHIFLMN 480

Query: 488 NIHYIVQKVENSDLIAFLGSGWMREHIRMFQRHATIYMRVTWQSVLSLLRLDGDGMKTSK 547
           NIHYIVQKVENS+L+AFLGSGWMREHIRMFQ HA IYMRVTWQSVLSLLRLDGDGMKT K
Sbjct: 481 NIHYIVQKVENSELVAFLGSGWMREHIRMFQSHAIIYMRVTWQSVLSLLRLDGDGMKTPK 540

Query: 548 AVFKEKYRAFNAAFEEIYKNQTGWNVPDPQLRDDLLIQTSNCVIQAYRILCGSRCQFNRE 607
           AVFKEKYRAFNAAFEEIYKNQTGWNVPDPQLRDDLLIQTSNCVIQAYRIL  SR Q NRE
Sbjct: 541 AVFKEKYRAFNAAFEEIYKNQTGWNVPDPQLRDDLLIQTSNCVIQAYRILYESRSQSNRE 600

Query: 608 KYIKYTTDDLSKHMLDLLQGSSRSLQSSRRR 638
           KYIKYTTDDLSKHMLDLLQGSS SLQSSRRR
Sbjct: 601 KYIKYTTDDLSKHMLDLLQGSSCSLQSSRRR 631

BLAST of Chy12G220980 vs. NCBI nr
Match: XP_038893966.1 (exocyst complex component EXO70E2 [Benincasa hispida])

HSP 1 Score: 1135 bits (2936), Expect = 0.0
Identity = 568/638 (89.03%), Postives = 592/638 (92.79%), Query Frame = 0

Query: 1   MGDCESIIPTSEGEQHALAAFQHLVKFLRTSQNVNNELKKLLADLDSHLTSFTSYTHLEV 60
           MGDCESIIPTSEGEQH LA+FQHLVKFLRTSQNVN+ELK+LLADLDSHL + T YTHLEV
Sbjct: 1   MGDCESIIPTSEGEQHVLASFQHLVKFLRTSQNVNDELKRLLADLDSHLNTMTLYTHLEV 60

Query: 61  GKLSELETRFKSAEEKITRWESNKSMIWDSDPKEACEYMKAVDEIHKVQEGLRSLSANDS 120
           GKLSELETR KSAEEKI RWESNKSMIWDS  KEACEYMKAVDEIH VQEGLRSLSANDS
Sbjct: 61  GKLSELETRLKSAEEKIRRWESNKSMIWDSCSKEACEYMKAVDEIHMVQEGLRSLSANDS 120

Query: 121 QKQNELLFQADSVVQIAMARLEQELIHILAQHKLYFEPDYVPFQSGGYDVVYSESFVSAE 180
           QKQNELLFQADSVVQIAMARLEQEL+HILAQHKLYFEPDYVPFQSGGYDVVY+ESFVS E
Sbjct: 121 QKQNELLFQADSVVQIAMARLEQELVHILAQHKLYFEPDYVPFQSGGYDVVYNESFVSTE 180

Query: 181 DSLEVETSHGESIPALYAVNSVHPHVIAHLKSIANVMFTSNYIQEFCLTFVKMRKDALNE 240
           DS+E ETSH ESIPA+Y VN VHPHVI HLKSIANVMFTSNYIQEFCL FVKMRKDALNE
Sbjct: 181 DSMEEETSHRESIPAVYVVNLVHPHVIPHLKSIANVMFTSNYIQEFCLAFVKMRKDALNE 240

Query: 241 YLFILEMEKYSIGDLLTMEWSDLNVKIKKWVWLIKIVIRVYLISEQRLCNNIFEGIGSYS 300
           YLFILEMEKYSI DLLTMEW  LNVKIKKWVWLIKIVIRVYL SEQRLCNNIFEGI SYS
Sbjct: 241 YLFILEMEKYSIADLLTMEWGVLNVKIKKWVWLIKIVIRVYLTSEQRLCNNIFEGIESYS 300

Query: 301 AVCFTEISATSMLRLLNFGEAIAMEPHRPEKLFHLLDMYEVLKNLLGFVDELFSEETEKG 360
           AVCFTEISATSML LLNFGEAIAMEPHRPEKLFHLLDMYEVLKNLLG++D LFSEETEKG
Sbjct: 301 AVCFTEISATSMLHLLNFGEAIAMEPHRPEKLFHLLDMYEVLKNLLGYIDALFSEETEKG 360

Query: 361 SFLKFEFHNLFKKLGDSARATFLDFGNFIACSISTNPFPGGGVHHLTRYVMNYIHTLTVF 420
           SFLK EFHNLFKKLGDSARATF+DFG+FIACSISTNPFPGGGVHHLTRYVMN+I TLTV+
Sbjct: 361 SFLKVEFHNLFKKLGDSARATFVDFGSFIACSISTNPFPGGGVHHLTRYVMNFIKTLTVY 420

Query: 421 RDSLVILLQDQAADDLSPTSELQSEINSIPCPMAYHLQSIISHLLSNLNNKSKLYKDGAL 480
           RDSLV LLQD A D LSP SELQ+EINS+PCPMAYH QSI SHL+SNLNNKSKLYKD AL
Sbjct: 421 RDSLVFLLQDNATDGLSPASELQNEINSMPCPMAYHFQSITSHLISNLNNKSKLYKDDAL 480

Query: 481 RHIFLMNNIHYIVQKVENSDLIAFLGSGWMREHIRMFQRHATIYMRVTWQSVLSLLRLDG 540
           RHIFLMNNIHY+VQKVE+S+L AFLGSGWMREH RMFQ H TIY+RVTW SVLSLLRLDG
Sbjct: 481 RHIFLMNNIHYMVQKVEDSELKAFLGSGWMREHTRMFQNHGTIYVRVTWHSVLSLLRLDG 540

Query: 541 DGMKTSKAVFKEKYRAFNAAFEEIYKNQTGWNVPDPQLRDDLLIQTSNCVIQAYRILCGS 600
           DG+ T KAVFKEKYRAFNAAFEEIYKNQTGW VPDP+L DDLLIQTSNCVIQAYRILC S
Sbjct: 541 DGLNTPKAVFKEKYRAFNAAFEEIYKNQTGWIVPDPRLCDDLLIQTSNCVIQAYRILCES 600

Query: 601 RCQFNREKYIKYTTDDLSKHMLDLLQGSSRSLQSSRRR 638
           R Q+NREKYIKYTTDDLSKHMLDL QGS RSLQSSRRR
Sbjct: 601 RSQYNREKYIKYTTDDLSKHMLDLFQGSPRSLQSSRRR 638

BLAST of Chy12G220980 vs. NCBI nr
Match: XP_023520029.1 (exocyst complex component EXO70E2 [Cucurbita pepo subsp. pepo] >XP_023520030.1 exocyst complex component EXO70E2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1013 bits (2620), Expect = 0.0
Identity = 509/638 (79.78%), Postives = 553/638 (86.68%), Query Frame = 0

Query: 1   MGDCESIIPTSEGEQHALAAFQHLVKFLRTSQNVNNELKKLLADLDSHLTSFTSYTHLEV 60
           MGDCESIIPTSEGEQH +AAFQHL K LRTSQNVN+ELK+LL DLDSHLTS T YTHLE 
Sbjct: 1   MGDCESIIPTSEGEQHVIAAFQHLAKLLRTSQNVNDELKRLLTDLDSHLTSMTLYTHLED 60

Query: 61  GKLSELETRFKSAEEKITRWESNKSMIWDSDPKEACEYMKAVDEIHKVQEGLRSLSANDS 120
           GKL+ELE+RFKSAEEKI RWESN+SMIWDS PKEACEYMKAVDEIHKVQEGL SL  ND 
Sbjct: 61  GKLNELESRFKSAEEKIRRWESNESMIWDSGPKEACEYMKAVDEIHKVQEGLISLPVNDG 120

Query: 121 QKQNELLFQADSVVQIAMARLEQELIHILAQHKLYFEPDYVPFQSGGYDVVYSESFVSAE 180
           +KQ +LL QA+SV+Q AMARLEQEL+HILAQHKLYFEP+ VPFQSGGYDVVY+ESF S E
Sbjct: 121 RKQKDLLCQAESVIQFAMARLEQELVHILAQHKLYFEPNCVPFQSGGYDVVYNESFASVE 180

Query: 181 DSLEVETSHGESIPALYAVNSVHPHVIAHLKSIANVMFTSNYIQEFCLTFVKMRKDALNE 240
           DSLE ETS  ESIPA+  VN VHPHVI HLKSIAN MFTSNYIQEF L FV+MRKDAL+E
Sbjct: 181 DSLEEETSQRESIPAVCIVNMVHPHVIPHLKSIANAMFTSNYIQEFSLAFVRMRKDALDE 240

Query: 241 YLFILEMEKYSIGDLLTMEWSDLNVKIKKWVWLIKIVIRVYLISEQRLCNNIFEGIGSYS 300
           YL ILEME++SIGDL TMEW  LNVKIKKW+WLIK+VIRVYL SEQRLCNNIFEG GSYS
Sbjct: 241 YLLILEMERHSIGDLQTMEWDVLNVKIKKWIWLIKVVIRVYLASEQRLCNNIFEGFGSYS 300

Query: 301 AVCFTEISATSMLRLLNFGEAIAMEPHRPEKLFHLLDMYEVLKNLLGFVDELFSEETEKG 360
           A CF EISATSML LLNFGEAIAM   RPEKLF LLDMYEVL+NLLG +  LFSE+TE G
Sbjct: 301 ATCFAEISATSMLCLLNFGEAIAMTTRRPEKLFRLLDMYEVLENLLGDIGALFSEDTEMG 360

Query: 361 SFLKFEFHNLFKKLGDSARATFLDFGNFIACSISTNPFPGGGVHHLTRYVMNYIHTLTVF 420
           SFLK EFHNLF KL DSARATF+DFG+ IA SIST+PFPGGGVHHLT+YVMNYI TLTV+
Sbjct: 361 SFLKAEFHNLFMKLSDSARATFVDFGSLIARSISTSPFPGGGVHHLTKYVMNYIRTLTVY 420

Query: 421 RDSLVILLQDQAADDLSPTSELQSEINSIPCPMAYHLQSIISHLLSNLNNKSKLYKDGAL 480
           RDSLV LL+D+AAD L P SEL +EINS+PCP+A+HLQSI S L+SNLNNKSKLY D AL
Sbjct: 421 RDSLVFLLRDEAADGLGPASELLNEINSMPCPLAFHLQSITSQLISNLNNKSKLYNDDAL 480

Query: 481 RHIFLMNNIHYIVQKVENSDLIAFLGSGWMREHIRMFQRHATIYMRVTWQSVLSLLRLDG 540
           RHIFLMNNIHY+VQKV+ S+L AF G  W+REH RM Q HA IYMRVTWQSV+SLL+LDG
Sbjct: 481 RHIFLMNNIHYMVQKVKGSELRAFFGGEWIREHNRMLQIHANIYMRVTWQSVISLLKLDG 540

Query: 541 DGMKTSKAVFKEKYRAFNAAFEEIYKNQTGWNVPDPQLRDDLLIQTSNCVIQAYRILCGS 600
           DG  T KAVFKEKYRAFN AFEEIYKNQTGWNV DP+LRDDL I TSNCVIQAYR LC  
Sbjct: 541 DGTNTPKAVFKEKYRAFNVAFEEIYKNQTGWNVSDPELRDDLHILTSNCVIQAYRFLCNL 600

Query: 601 RCQFNREKYIKYTTDDLSKHMLDLLQGSSRSLQSSRRR 638
           + QFN EKYIKYT DDLSKH+LDL QGS RSLQSSRRR
Sbjct: 601 KSQFNCEKYIKYTIDDLSKHILDLFQGSPRSLQSSRRR 638

BLAST of Chy12G220980 vs. TAIR 10
Match: AT5G61010.1 (exocyst subunit exo70 family protein E2 )

HSP 1 Score: 506.5 bits (1303), Expect = 3.1e-143
Identity = 284/645 (44.03%), Postives = 403/645 (62.48%), Query Frame = 0

Query: 1   MGDCESIIPTSEGEQHALAAFQHLVKFLRTS-QNVNNELKKLLADLDSHLTSF-TSYTHL 60
           M + +S +P S    H   A  H+VK LR S  N++  L+KLL+DL+ HL++F  + T +
Sbjct: 1   MAEFDSKVPVSGMNNHVFEACHHVVKALRASDNNLDANLRKLLSDLEMHLSTFGIADTKV 60

Query: 61  EVGKLSELETRFKSAEEKITRWESNKSMIWDSDPKEACEYMKAVDEIHKVQEGLRSLSAN 120
           E    SE++ RFK A ++I  WE+N+S ++++   EA ++ +A+ ++  V  G ++L   
Sbjct: 61  EDAGFSEIKKRFKEAVKRIRSWETNQSTMFEAGLSEADQFFQALYDVQTVLVGFKALPMK 120

Query: 121 DSQKQNELLFQADSVVQIAMARLEQELIHILAQHKLYFEPDYVPFQSGGYDVVYSESFVS 180
            +Q + ++  QA   + IAM RLE+EL  +L QHK + +PDY+   S   D+VY ESFVS
Sbjct: 121 TNQMEKDVYNQATVALDIAMLRLEKELCDVLHQHKRHVQPDYLAVSSRRKDIVYDESFVS 180

Query: 181 AEDSLEVETS---HGESIPALYAVNSVHPHVIAHLKSIANVMFTSNYIQEFCLTFVKMRK 240
            +D + VE S     E I   Y  + V P V+ H+K+IAN MF   Y Q FC  F+ +++
Sbjct: 181 LDDEVIVEASSHEDDEQISDFYNSDLVDPIVLPHIKAIANAMFACEYDQPFCEAFIGVQR 240

Query: 241 DALNEYLFILEMEKYSIGDLLTMEWSDLNVKIKKWVWLIKIVIRVYLISEQRLCNNIFEG 300
           +AL EY+  LEME++S  D+L M+W DLN  ++KW  ++KI+ +VYL SE++LC+ I   
Sbjct: 241 EALEEYMVTLEMERFSCVDVLRMDWEDLNGAMRKWTKVVKIITQVYLASEKQLCDQILGD 300

Query: 301 IGSYSAVCFTEISATSMLRLLNFGEAIAMEPHRPEKLFHLLDMYEVLKNLLGFVDELFSE 360
             S S  CF EIS  ++L LLNFGEA+ +   +PE L   L MYEV   +L  VD LF +
Sbjct: 301 FESISTACFIEISKDAILSLLNFGEAVVLRSCKPEMLERFLSMYEVSAEILVDVDNLFPD 360

Query: 361 ETEKGSFLKFEFHNLFKKLGDSARATFLDFGNFIACSISTNPFPGGGVHHLTRYVMNYIH 420
           ET  GS L+  FHNL KKL D    TFL F + IA   ST PF GGG+HHLTRYVMNY+ 
Sbjct: 361 ET--GSSLRIAFHNLSKKLADHTTTTFLKFKDAIASDESTRPFHGGGIHHLTRYVMNYLK 420

Query: 421 TLTVFRDSLVILLQDQAADDLSPTSELQSEINSIPCPMAYHLQSIISHLLSNLNNKSKLY 480
            L  + DSL  LLQ+   DD  P    +  + S   PMA HL+SI++ L S+L  K++LY
Sbjct: 421 LLPEYTDSLNSLLQNIHVDDSIPEKTGEDVLPSTFSPMARHLRSIVTTLESSLERKAQLY 480

Query: 481 KDGALRHIFLMNNIHYIVQKVENSDLIAFLGSGWMREHIRMFQRHATIYMRVTWQSVLSL 540
            D AL+ IFLMNN  Y+VQKV+ S+L    G  W+R+HI  +Q + T Y R TW S+L+L
Sbjct: 481 ADEALKSIFLMNNFRYMVQKVKGSELRRLFGDEWIRKHIASYQCNVTNYERSTWSSILAL 540

Query: 541 LRLDGDGMKTSKAVFKEKYRAFNAAFEEIYKNQTGWNVPDPQLRDDLLIQTSNCVIQAYR 600
           LR + D ++T     +E+ R F+ AF+++YKNQT W+VPD +LRDDL I TS  V+Q+YR
Sbjct: 541 LRDNNDSVRT----LRERCRLFSLAFDDVYKNQTRWSVPDSELRDDLHISTSVKVVQSYR 600

Query: 601 ILCGSRCQFNREKYIKYTTDDLSKHMLDLLQ--GSSRSLQSSRRR 639
              G       EK+I+YT +D+   +LDL +   S RSL+SSR+R
Sbjct: 601 GFLGRNAVRIGEKHIRYTCEDIENMLLDLFECLPSPRSLRSSRKR 639

BLAST of Chy12G220980 vs. TAIR 10
Match: AT5G61010.2 (exocyst subunit exo70 family protein E2 )

HSP 1 Score: 506.5 bits (1303), Expect = 3.1e-143
Identity = 284/645 (44.03%), Postives = 403/645 (62.48%), Query Frame = 0

Query: 1   MGDCESIIPTSEGEQHALAAFQHLVKFLRTS-QNVNNELKKLLADLDSHLTSF-TSYTHL 60
           M + +S +P S    H   A  H+VK LR S  N++  L+KLL+DL+ HL++F  + T +
Sbjct: 1   MAEFDSKVPVSGMNNHVFEACHHVVKALRASDNNLDANLRKLLSDLEMHLSTFGIADTKV 60

Query: 61  EVGKLSELETRFKSAEEKITRWESNKSMIWDSDPKEACEYMKAVDEIHKVQEGLRSLSAN 120
           E    SE++ RFK A ++I  WE+N+S ++++   EA ++ +A+ ++  V  G ++L   
Sbjct: 61  EDAGFSEIKKRFKEAVKRIRSWETNQSTMFEAGLSEADQFFQALYDVQTVLVGFKALPMK 120

Query: 121 DSQKQNELLFQADSVVQIAMARLEQELIHILAQHKLYFEPDYVPFQSGGYDVVYSESFVS 180
            +Q + ++  QA   + IAM RLE+EL  +L QHK + +PDY+   S   D+VY ESFVS
Sbjct: 121 TNQMEKDVYNQATVALDIAMLRLEKELCDVLHQHKRHVQPDYLAVSSRRKDIVYDESFVS 180

Query: 181 AEDSLEVETS---HGESIPALYAVNSVHPHVIAHLKSIANVMFTSNYIQEFCLTFVKMRK 240
            +D + VE S     E I   Y  + V P V+ H+K+IAN MF   Y Q FC  F+ +++
Sbjct: 181 LDDEVIVEASSHEDDEQISDFYNSDLVDPIVLPHIKAIANAMFACEYDQPFCEAFIGVQR 240

Query: 241 DALNEYLFILEMEKYSIGDLLTMEWSDLNVKIKKWVWLIKIVIRVYLISEQRLCNNIFEG 300
           +AL EY+  LEME++S  D+L M+W DLN  ++KW  ++KI+ +VYL SE++LC+ I   
Sbjct: 241 EALEEYMVTLEMERFSCVDVLRMDWEDLNGAMRKWTKVVKIITQVYLASEKQLCDQILGD 300

Query: 301 IGSYSAVCFTEISATSMLRLLNFGEAIAMEPHRPEKLFHLLDMYEVLKNLLGFVDELFSE 360
             S S  CF EIS  ++L LLNFGEA+ +   +PE L   L MYEV   +L  VD LF +
Sbjct: 301 FESISTACFIEISKDAILSLLNFGEAVVLRSCKPEMLERFLSMYEVSAEILVDVDNLFPD 360

Query: 361 ETEKGSFLKFEFHNLFKKLGDSARATFLDFGNFIACSISTNPFPGGGVHHLTRYVMNYIH 420
           ET  GS L+  FHNL KKL D    TFL F + IA   ST PF GGG+HHLTRYVMNY+ 
Sbjct: 361 ET--GSSLRIAFHNLSKKLADHTTTTFLKFKDAIASDESTRPFHGGGIHHLTRYVMNYLK 420

Query: 421 TLTVFRDSLVILLQDQAADDLSPTSELQSEINSIPCPMAYHLQSIISHLLSNLNNKSKLY 480
            L  + DSL  LLQ+   DD  P    +  + S   PMA HL+SI++ L S+L  K++LY
Sbjct: 421 LLPEYTDSLNSLLQNIHVDDSIPEKTGEDVLPSTFSPMARHLRSIVTTLESSLERKAQLY 480

Query: 481 KDGALRHIFLMNNIHYIVQKVENSDLIAFLGSGWMREHIRMFQRHATIYMRVTWQSVLSL 540
            D AL+ IFLMNN  Y+VQKV+ S+L    G  W+R+HI  +Q + T Y R TW S+L+L
Sbjct: 481 ADEALKSIFLMNNFRYMVQKVKGSELRRLFGDEWIRKHIASYQCNVTNYERSTWSSILAL 540

Query: 541 LRLDGDGMKTSKAVFKEKYRAFNAAFEEIYKNQTGWNVPDPQLRDDLLIQTSNCVIQAYR 600
           LR + D ++T     +E+ R F+ AF+++YKNQT W+VPD +LRDDL I TS  V+Q+YR
Sbjct: 541 LRDNNDSVRT----LRERCRLFSLAFDDVYKNQTRWSVPDSELRDDLHISTSVKVVQSYR 600

Query: 601 ILCGSRCQFNREKYIKYTTDDLSKHMLDLLQ--GSSRSLQSSRRR 639
              G       EK+I+YT +D+   +LDL +   S RSL+SSR+R
Sbjct: 601 GFLGRNAVRIGEKHIRYTCEDIENMLLDLFECLPSPRSLRSSRKR 639

BLAST of Chy12G220980 vs. TAIR 10
Match: AT3G29400.1 (exocyst subunit exo70 family protein E1 )

HSP 1 Score: 417.5 bits (1072), Expect = 1.9e-116
Identity = 268/661 (40.54%), Postives = 374/661 (56.58%), Query Frame = 0

Query: 12  EGEQHALAAFQHLVKFLRT----SQNVNNELKKLLADLDSHLTSFTSYTHLEVGKLSELE 71
           +GE+  + A ++LVK LR+    ++N  N L  LL +L   + +  +    E  ++ E+E
Sbjct: 7   DGEEKLIVAAKYLVKELRSGKSLTKNAKNVLGNLLLELSRVVIAEDTQDRDEEDEIGEIE 66

Query: 72  TRFKSAEEKITRWESNKSMIWDSDPKEACEYMKAVDEIHKVQEGLRSLSANDSQKQNELL 131
            R     EKI   E ++SMIWD    E   Y+ AV+E       LRSL       +   L
Sbjct: 67  ERLNVVSEKIMTREVDESMIWDLGSDEGNLYLDAVNE-------LRSLIDRLDGSEELSL 126

Query: 132 FQADSVVQIAMARLEQELIHILAQHKLYFEPDYVPFQS----GGYDVVYSESF--VSAED 191
            +A  V+QIAMARLE E  H+L +++L FE ++  F+S     G +     SF   S ED
Sbjct: 127 RKAHDVLQIAMARLEDEFKHLLVENRLPFELEHSSFRSIEADHGVEEESMASFGAASTED 186

Query: 192 SLEVETSHGESIPALYAVNSVHPHVIAHLKSIANVMFTSNYIQEFCLTFVKMRKDALNEY 251
            +    +          V+ V+P VI  LK+IAN M  S Y +E       +RKDAL+E+
Sbjct: 187 LILGSNNDSRRNSGDVVVDLVNPDVILDLKNIANTMIASGYDRECIQVCTMVRKDALDEF 246

Query: 252 LFILEMEKYSIGDLLTMEWSDLNVKIKKWVWLIKIVIRVYLISEQRLCNNIFEGIGSYSA 311
           L+  E+EK SI D+L M+W+ LN  IKKWV +++ +++VYL+SE+ L N IF  +     
Sbjct: 247 LYNHEVEKLSIEDVLRMDWATLNTNIKKWVRVMRDIVQVYLLSEKSLDNQIFGDLNEIGL 306

Query: 312 VCFTEISATSMLRLLNFGEAIAMEPHRPEKLFHLLDMYEVLKNLLGFVDELFSEETEKGS 371
            CF +     M++LLNFGEA+++ P +PEKL  +L+MYE+   LL  +D LF +    GS
Sbjct: 307 TCFVDTVKAPMMQLLNFGEAVSLGPRQPEKLLRILEMYELASELLPEIDALFLD--HPGS 366

Query: 372 FLKFEFHNLFKKLGDSARATFLDFGNFIACSISTNPFPGGGVHHLTRYVMNYIHTLTVFR 431
            ++ E+  + ++LGD AR TFL+F + IA  +S++PFPGG VH LT YVMNY+  LT F+
Sbjct: 367 SVRTEYREVMRRLGDCARTTFLEFKSAIAADVSSHPFPGGAVHPLTNYVMNYLMALTDFK 426

Query: 432 DSLVILL--QDQAADDLSPTS-----------ELQSEINSIP---CPMAYHLQSIISHLL 491
            +L  LL   D A D   P S           E   E +S P     M  H  SI S L 
Sbjct: 427 HTLDSLLMEHDDAEDLTIPPSPDIINPVMVEEESTYENSSSPEKFLAMTRHFYSITSVLE 486

Query: 492 SNLNNKSKLYKDGALRHIFLMNNIHYIVQKVENSDLIAFLGSGWMREHIRMFQRHATIYM 551
           +NL  KSKLYKD +L+HIFL+NNIHY+ +KV  S+L    G  W R+H   FQ+ AT Y 
Sbjct: 487 ANLQEKSKLYKDVSLQHIFLLNNIHYMTRKVLKSELRHIFGDKWNRKHTWKFQQQATEYE 546

Query: 552 RVTWQSVLSLLRLDGDGM-------KTSKAVF-KEKYRAFNAAFEEIYKNQTGWNVPDPQ 611
           R TW  VLS L+ DG G          SK +  +E+++ FN AFEE+YK QTGW + D  
Sbjct: 547 RATWLPVLSFLKDDGSGSGPGSGSGSGSKNLRPRERFQGFNTAFEEVYKAQTGWLISDEG 606

Query: 612 LRDDLLIQTSNCVIQAYRILCGSRCQFNREKYIKYTTDDLSKHMLDLLQGSSRSLQSSRR 639
           LR+D+  + S  VIQAY            E+YIKYTTDD+ + +LDL  GSS+SL +S R
Sbjct: 607 LREDVRTKASMWVIQAYWTFYSRHKNSVSERYIKYTTDDIERLLLDLFAGSSKSLNNSYR 658

BLAST of Chy12G220980 vs. TAIR 10
Match: AT5G50380.1 (exocyst subunit exo70 family protein F1 )

HSP 1 Score: 362.5 bits (929), Expect = 7.2e-100
Identity = 233/676 (34.47%), Postives = 366/676 (54.14%), Query Frame = 0

Query: 14  EQHALAAFQHLVKFLRTSQNVNNELKKLLADLDSHLTSF-TSYTHLEVGKLSELETRFKS 73
           E   LA  Q ++K L T + V  ++  + +  D+ L++  T+ T+ E  +   L  R ++
Sbjct: 23  EDRVLATAQQIMKSLNTPKEVREDMLLIFSSFDNRLSNIKTAMTNQEENQNDALVARLEA 82

Query: 74  AEEKITRWE------------------SNKSMIWDSDPKEACEYMKAVDEIHKVQEGLRS 133
           AE  I RW+                  S+ S+ +D  P+EA E++ AVDEI  + E L  
Sbjct: 83  AESVIHRWDGGNDSSRHSSSSSGNYRSSSFSLSFDESPEEATEFLSAVDEIISLLEDL-- 142

Query: 134 LSANDSQKQNELLFQADSVVQIAMARLEQELIHILAQH-------KLYFEPDYVPFQSGG 193
                S+ + +++ +ADS +Q+AM++LE E   IL ++       +LY     V      
Sbjct: 143 ----SSENKPDMVDRADSALQMAMSQLEDEFRRILIRNTVPLDAERLYGSMRRVSLSFAD 202

Query: 194 YDVVYS-ESFVSAEDS------LEVETSHGESIPALYAVNSVHPHVIAHLKSIANVMFTS 253
            DVV   E+F    D         +    G SI     V+ ++P  +  LK IA  M  +
Sbjct: 203 GDVVEDFENFGLVADGDGSGSRRRLFHERGGSIGCDLWVDLINPTAVEDLKEIAERMIRA 262

Query: 254 NYIQEFCLTFVKMRKDALNEYLFILEMEKYSIGDLLTMEWSDLNVKIKKWVWLIKIVIRV 313
            Y +E    +  +R+DAL++ L IL +EK SI ++  ++W  ++ K+KKW+  +KI +RV
Sbjct: 263 GYEKECVQVYSSVRRDALDDCLMILGVEKLSIEEVQKIDWKSMDEKMKKWIQAVKITVRV 322

Query: 314 YLISEQRLCNNIFEGIGSYSAVCFTEISATSMLRLLNFGEAIAMEPHRPEKLFHLLDMYE 373
            L+ E+++C+ IF    S   VCF E + + +++LLNFGEA+A+     EKLF +LDMY+
Sbjct: 323 LLVGEKKICDEIFSSSESSKEVCFNETTKSCVMQLLNFGEAVAIGRRSSEKLFRILDMYD 382

Query: 374 VLKNLLGFVDELFSEETEKGSFLKFEFHNLFKKLGDSARATFLDFGNFIACSISTNPFPG 433
            L N+L  ++ + ++      F+  E   + + LGD+AR TF++F N +    S  P   
Sbjct: 383 ALANVLQTLEVMVTD-----CFVCNETKGVLEALGDAARGTFVEFENNVRNETSKRPTTN 442

Query: 434 GGVHHLTRYVMNYIHTLTVFRDSLVILLQDQAADDLSPTSELQSEINSIPCPMAYHLQSI 493
           G VH + RYVMNY+  +  +  +L  LL+   +  +S     +        P+A  +  +
Sbjct: 443 GEVHPMIRYVMNYMKLIVDYAVTLNSLLESNESSGVSGDDSTEE-----MSPLAKRILGL 502

Query: 494 ISHLLSNLNNKSKLYKDGALRHIFLMNNIHYIVQKVENSDLIAFLGSGWMREHIRMFQRH 553
           I+ L SNL +KSKLY+DG L+H+F+MNNI+YIVQKV++S+L   LG  W+R+     +++
Sbjct: 503 ITSLESNLEDKSKLYEDGGLQHVFMMNNIYYIVQKVKDSELGKLLGDDWVRKRRGQIRQY 562

Query: 554 ATIYMRVTWQSVLSLLRLDGDG-------------MKTSKAVFKEKYRAFNAAFEEIYKN 613
           AT Y+R +W  VLS LR +  G               +SK   KE++R FNA+FEE+Y+ 
Sbjct: 563 ATGYLRASWSRVLSALRDESMGGSSSGSPSYGQRSNNSSKMALKERFRGFNASFEELYRL 622

Query: 614 QTGWNVPDPQLRDDLLIQTSNCVIQAYRILCG-SRCQF----NREKYIKYTTDDLSKHML 639
           QT W VPDPQLR++L I  S  VI AYR   G +R Q     +  KYIKYT DDL  ++ 
Sbjct: 623 QTAWKVPDPQLREELRISISEKVIPAYRAFFGRNRSQLEGGRHAGKYIKYTPDDLESYLP 682

BLAST of Chy12G220980 vs. TAIR 10
Match: AT1G54090.1 (exocyst subunit exo70 family protein D2 )

HSP 1 Score: 303.9 bits (777), Expect = 3.0e-82
Identity = 211/626 (33.71%), Postives = 332/626 (53.04%), Query Frame = 0

Query: 64  SELETRFKSAEEKITRWESNKS------MIWDS--DPKEACEYMKAVDEIHKVQEGLRSL 123
           S+LET    AE+ I RW+S  S      +I+ S  D +E   Y+KAVDE   VQ  + S+
Sbjct: 11  SDLET----AEKIILRWDSTTSEEAKENLIFQSGGDREEVDRYLKAVDE---VQRHISSI 70

Query: 124 SANDSQKQNELLFQADSVVQIAMARLEQELIHILAQHKLYFEPDYVPFQSGGYDVVYSES 183
           S +D  K         + +QIAMARLE EL +IL      FEPD +   S     + S S
Sbjct: 71  SISDEVK---------ATIQIAMARLEDELRNILISQTSTFEPDSLLLDSS----LSSSS 130

Query: 184 FVSA----------------EDSLEVE--------------------TSHGESIPALYAV 243
           F S+                E+  +V+                     S+ +S  ++  +
Sbjct: 131 FASSSRTELEDDTCDDGNEEEEQQQVDLVLPDGSGSDSGSRRLSRSRRSNSKSTSSIREI 190

Query: 244 NSVHPHVIAHLKSIANVMFTSNYIQEFCLTFVKMRKDALNEYLFILEMEKYSIGDLLTME 303
           + V P  ++ L+SIA  M  + Y +E    +  +RK A+      L + K  IGD+  ++
Sbjct: 191 DLVTPEAVSDLRSIAQRMIGAGYSRECVQVYGNVRKSAMEMIFKQLGIVKLGIGDVQRLD 250

Query: 304 WSDLNVKIKKWVWLIKIVIRVYLISEQRLCNNIFEGIGSYSAVCFTEISATSMLRLLNFG 363
           W  + VKI++W+   K+ +RV   SE+RLC  IFE  G+    CF EI  TS L+L NF 
Sbjct: 251 WEAVEVKIRRWIRAAKVCVRVVFASEKRLCEQIFE--GTMEETCFMEIVKTSALQLFNFP 310

Query: 364 EAIAMEPHRPEKLFHLLDMYEVLKNLLGFVDELFSEETEKGSFLKFEFHNLFKKLGDSAR 423
           EAI++    PEKLF +LD+++ + +LL  ++E+F   + +   +  +   +  +L ++AR
Sbjct: 311 EAISISRRSPEKLFKILDLHDAITDLLPDMEEIFDSSSSES--ILVQATEIQSRLAEAAR 370

Query: 424 ATFLDFGNFIACSISTNPFPGGGVHHLTRYVMNYIHTLTVFRDSLVILLQDQAADDLSPT 483
               +F N +    S  P PGG +H LTRYVMNY++ ++ +R++L+ L+  +    L  T
Sbjct: 371 GILTEFENAVFREPSVVPVPGGTIHPLTRYVMNYLNLISDYRETLIDLVMTKPCRGLKCT 430

Query: 484 SELQ------SEINSIPCPMAYHLQSIISHLLSNLNNKSKLYKDGALRHIFLMNNIHYIV 543
           ++        SE+  I  P+A H+   +  L  NL  KS  Y+D  L HIF+MNN+HYIV
Sbjct: 431 NDRNDPDMDISELEGI-SPLALHMIWTMVMLQFNLEEKSLHYRDEPLSHIFVMNNVHYIV 490

Query: 544 QKVENS-DLIAFLGSGWMREHIRMFQRHATIYMRVTWQSVLSLLRLDGDGMK------TS 603
           QKV++S +L+  +G  ++R+   +F++ AT Y R TW  VL+ LR +G  +        S
Sbjct: 491 QKVKSSPELMELIGDKYLRKLTGIFRQAATKYQRATWVRVLNSLRDEGLHVSGSFSSGVS 550

Query: 604 KAVFKEKYRAFNAAFEEIYKNQTGWNVPDPQLRDDLLIQTSNCVIQAYRILCG---SRCQ 628
           K+  +E+++AFN  FEE+++ Q+ W+VPD QLR++L I  S  +I AYR   G      +
Sbjct: 551 KSALRERFKAFNTMFEEVHRIQSTWSVPDTQLREELRISLSEHLIPAYRSFLGRFRGHIE 610

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FNR34.3e-14244.03Exocyst complex component EXO70E2 OS=Arabidopsis thaliana OX=3702 GN=EXO70E2 PE=... [more]
Q9FGH91.7e-6628.46Exocyst complex component EXO70B1 OS=Arabidopsis thaliana OX=3702 GN=EXO70B1 PE=... [more]
Q9LZD33.3e-6528.79Exocyst complex component EXO70A1 OS=Arabidopsis thaliana OX=3702 GN=EXO70A1 PE=... [more]
Q9LMJ42.8e-5628.72Exocyst complex component EXO70B2 OS=Arabidopsis thaliana OX=3702 GN=EXO70B2 PE=... [more]
F4KG581.3e-5029.15Exocyst complex component EXO70A3 OS=Arabidopsis thaliana OX=3702 GN=EXO70A3 PE=... [more]
Match NameE-valueIdentityDescription
A0A1S4DU880.0e+0093.26Exocyst subunit Exo70 family protein OS=Cucumis melo OX=3656 GN=LOC103491934 PE=... [more]
A0A5D3BIJ10.0e+0093.19Exocyst subunit Exo70 family protein OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A6J1E6T26.6e-28778.68Exocyst subunit Exo70 family protein OS=Cucurbita moschata OX=3662 GN=LOC1114313... [more]
A0A6J1KLM62.5e-28678.53Exocyst subunit Exo70 family protein OS=Cucurbita maxima OX=3661 GN=LOC111495300... [more]
A0A6J1C7N52.1e-28577.31Exocyst subunit Exo70 family protein OS=Momordica charantia OX=3673 GN=LOC111008... [more]
Match NameE-valueIdentityDescription
XP_004137600.10.096.08exocyst complex component EXO70E2 [Cucumis sativus] >XP_031744501.1 exocyst comp... [more]
XP_008450312.10.093.26PREDICTED: exocyst complex component EXO70A1 [Cucumis melo] >XP_008450459.1 PRED... [more]
TYJ98937.10.093.19exocyst complex component EXO70A1 [Cucumis melo var. makuwa][more]
XP_038893966.10.089.03exocyst complex component EXO70E2 [Benincasa hispida][more]
XP_023520029.10.079.78exocyst complex component EXO70E2 [Cucurbita pepo subsp. pepo] >XP_023520030.1 e... [more]
Match NameE-valueIdentityDescription
AT5G61010.13.1e-14344.03exocyst subunit exo70 family protein E2 [more]
AT5G61010.23.1e-14344.03exocyst subunit exo70 family protein E2 [more]
AT3G29400.11.9e-11640.54exocyst subunit exo70 family protein E1 [more]
AT5G50380.17.2e-10034.47exocyst subunit exo70 family protein F1 [more]
AT1G54090.13.0e-8233.71exocyst subunit exo70 family protein D2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 63..83
NoneNo IPR availableGENE3D1.20.1280.170Exocyst complex component Exo70coord: 75..635
e-value: 9.5E-145
score: 485.3
NoneNo IPR availablePANTHERPTHR12542:SF92EXOCYST COMPLEX COMPONENT EXO70E2coord: 23..632
IPR004140Exocyst complex component Exo70PFAMPF03081Exo70coord: 268..622
e-value: 2.3E-104
score: 349.4
IPR004140Exocyst complex component Exo70PANTHERPTHR12542EXOCYST COMPLEX PROTEIN EXO70coord: 23..632
IPR016159Cullin repeat-like-containing domain superfamilySUPERFAMILY74788Cullin repeat-likecoord: 85..624

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy12G220980.1Chy12G220980.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006887 exocytosis
biological_process GO:0015031 protein transport
cellular_component GO:0000145 exocyst