Chy12G219560 (gene) Cucumber (hystrix) v1

Overview
NameChy12G219560
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionGRAM domain-containing protein
LocationchrH12: 17819178 .. 17820878 (-)
RNA-Seq ExpressionChy12G219560
SyntenyChy12G219560
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAATCTTTCGACAAAACGCCGGATAGGAACTCGTCTTTTTCTGAACCTGTCAGCTCAATCCACAGTCCTCTTTATTCCGATGGCTCTCCTTCTTCTTCCGGTAAAAATCACTCTTCTCTCGATCTTGGCGCCGTTCTGTTCATCTAAGATTGATGGGGTAGTCATTTTTTGCAGGTAATGATAATTCAAAATCGGGGCGTAATAAACAATTCATCAGGAAACGAGGTGGATTTGTATTTCGGGTATACGAGCATGGTAAGGATCGATAGTTTTTGCTGTACAAATGTATTGAACACTACCTGTTTGTGGAAATGCCATCTGAAATTTATCACAATATGATATGATGGAATGAGGGTAATCTTGTGTTTGTGTGAAATCTGTAGTTAAGTTGGGGCCTAAATTCTTGGTAACTGCTAAAGGTAAATTGAGACTAGGGGCAAAAATTATACAACAAGGTGGCAGAAAGAACATATTCAAACAGGTTTTTGGAATTGTAGAAGGAGAGCAGCTTTTGAAGGCCTCTCAATGCTACTTATCAACAAGTGCAGGGCCCATTGCTGGGCTTCTTTTCATATCTACAGAGAAGGTTGCTTTCTGCAGTGAGCAAAGTATCACTTTCTCTTCACCAACTGGAGAATTGCTCAAAACACCTTATAAGGTAATCTCTTTGCACTTAAGCATTCGACTCAGGGTCGGAAAGATGTAAAACATTTATTCTTTTAGACACGTAGGAGATTGCACACAAGTCATGTTTGATTGATTTGATTTGACCATATATATAACATAAAGTTCTGGTTTTCAGAAAATGGAAGTCAAAGATTTTAGGTGTAGAATGGAAGATACACATATAGATACTGACAATTTTGGCTGGTCTTTTTATATAAAAGATCACCAACTGTTCGTTGGGAGAAAGTGATTGAACTAACTGAAAAGTGTCTAGGTGAAAGCTATGATGTAACTATCCATATGTGCTTAGTTCTTATCAAGAATTTTGATGAAGCCTTGGGATCTAGGACCAAGATCGTAATTTCGAACTATCTAATCTGTGAAAGTTTGAACACCATACGGTTCAAAACTTCCATGAGATGCAAAATATTACAAGCTAACCTTTTAAGATATATAATACTTCCAAATTAAAGGGGTATATTAAGACCCCTGCTTTGCAGTCTGATGACTTTCCCCCCTTACATAAAGGTGTATTTAAATAAAGTTCTTTCTTGAGAGTAATTTAAAACTCCACAATCTTTATTTGTATTAAAAGCAAAAAGGGAAAAGAGTTTAACTTTCAAAAAGCTGAGAAGGATCTCTCTAGCACTAAACTACCACTTTTCCCAACCTGTTAGGGCATGTTTACTCTTGTTAGGCCCAAAATCAATAAAAAATGCATGACGAAAGTGACCTTAGTCACTCAAAATTACTTCCAAACATGCAATTCATACCTGCTAAAGATCATACCCTTTGAAAATGACAGTGGAGTGTGATTTTCTACAGGTTTTGATACCATTAAAGAAGATCAGAAAAGCCAATCAAAGTGAGAATGTTAATGATCCAGCAAAAAAGTACATCGAAGTGGTGACGGATGACAACTTTGATTTCTGGTTTATGGGATTTTTGAGATATGAAAAGGCATTTACAAATCTTCAGAAGGCCATTTCCATGGCTAACAATTCAATGCAACAGTCCACAGATATATCATGA

mRNA sequence

ATGAAATCTTTCGACAAAACGCCGGATAGGAACTCGTCTTTTTCTGAACCTGTCAGCTCAATCCACAGTCCTCTTTATTCCGATGGCTCTCCTTCTTCTTCCGGTAATGATAATTCAAAATCGGGGCGTAATAAACAATTCATCAGGAAACGAGGTGGATTTGTATTTCGGGTATACGAGCATGTTAAGTTGGGGCCTAAATTCTTGGTAACTGCTAAAGGTAAATTGAGACTAGGGGCAAAAATTATACAACAAGGTGGCAGAAAGAACATATTCAAACAGGTTTTTGGAATTGTAGAAGGAGAGCAGCTTTTGAAGGCCTCTCAATGCTACTTATCAACAAGTGCAGGGCCCATTGCTGGGCTTCTTTTCATATCTACAGAGAAGGTTGCTTTCTGCAGTGAGCAAAGTATCACTTTCTCTTCACCAACTGGAGAATTGCTCAAAACACCTTATAAGGTTTTGATACCATTAAAGAAGATCAGAAAAGCCAATCAAAGTGAGAATGTTAATGATCCAGCAAAAAAGTACATCGAAGTGGTGACGGATGACAACTTTGATTTCTGGTTTATGGGATTTTTGAGATATGAAAAGGCATTTACAAATCTTCAGAAGGCCATTTCCATGGCTAACAATTCAATGCAACAGTCCACAGATATATCATGA

Coding sequence (CDS)

ATGAAATCTTTCGACAAAACGCCGGATAGGAACTCGTCTTTTTCTGAACCTGTCAGCTCAATCCACAGTCCTCTTTATTCCGATGGCTCTCCTTCTTCTTCCGGTAATGATAATTCAAAATCGGGGCGTAATAAACAATTCATCAGGAAACGAGGTGGATTTGTATTTCGGGTATACGAGCATGTTAAGTTGGGGCCTAAATTCTTGGTAACTGCTAAAGGTAAATTGAGACTAGGGGCAAAAATTATACAACAAGGTGGCAGAAAGAACATATTCAAACAGGTTTTTGGAATTGTAGAAGGAGAGCAGCTTTTGAAGGCCTCTCAATGCTACTTATCAACAAGTGCAGGGCCCATTGCTGGGCTTCTTTTCATATCTACAGAGAAGGTTGCTTTCTGCAGTGAGCAAAGTATCACTTTCTCTTCACCAACTGGAGAATTGCTCAAAACACCTTATAAGGTTTTGATACCATTAAAGAAGATCAGAAAAGCCAATCAAAGTGAGAATGTTAATGATCCAGCAAAAAAGTACATCGAAGTGGTGACGGATGACAACTTTGATTTCTGGTTTATGGGATTTTTGAGATATGAAAAGGCATTTACAAATCTTCAGAAGGCCATTTCCATGGCTAACAATTCAATGCAACAGTCCACAGATATATCATGA

Protein sequence

MKSFDKTPDRNSSFSEPVSSIHSPLYSDGSPSSSGNDNSKSGRNKQFIRKRGGFVFRVYEHVKLGPKFLVTAKGKLRLGAKIIQQGGRKNIFKQVFGIVEGEQLLKASQCYLSTSAGPIAGLLFISTEKVAFCSEQSITFSSPTGELLKTPYKVLIPLKKIRKANQSENVNDPAKKYIEVVTDDNFDFWFMGFLRYEKAFTNLQKAISMANNSMQQSTDIS*
Homology
BLAST of Chy12G219560 vs. ExPASy Swiss-Prot
Match: Q9FMW4 (Putative GEM-like protein 8 OS=Arabidopsis thaliana OX=3702 GN=At5g23370 PE=3 SV=1)

HSP 1 Score: 189.5 bits (480), Expect = 4.1e-47
Identity = 95/172 (55.23%), Postives = 126/172 (73.26%), Query Frame = 0

Query: 41  SGRNKQFIRKR--GGFVFRVYEHVKLGPKFLVTAKGKLRLGAKIIQQGGRKNIFKQVFGI 100
           +G+ K  +RK+    F   V +  KLGPK   T K KL LGA+I+Q GG + I+K++F +
Sbjct: 45  TGKGKSMLRKKKNDSFTNGVRDQDKLGPKLTETVKRKLSLGARILQMGGLEKIYKRLFKV 104

Query: 101 VEGEQLLKASQCYLSTSAGPIAGLLFISTEKVAFCSEQSITFSSPTGELLKTPYKVLIPL 160
            + E+L KA QCYLST+AGPIAGLLFIS++K+AFCSE+SI  +SP GEL +  YKV IPL
Sbjct: 105 SDEEKLFKAYQCYLSTTAGPIAGLLFISSKKIAFCSERSIKVASPQGELNRVHYKVSIPL 164

Query: 161 KKIRKANQSENVNDPAKKYIEVVTDDNFDFWFMGFLRYEKAFTNLQKAISMA 211
            KI   NQS+N   P++KY+EVVT D FDFWFMGFL Y+KAF  L++A+S++
Sbjct: 165 CKINGVNQSQNTTKPSQKYLEVVTVDGFDFWFMGFLSYQKAFNCLEQALSLS 216

BLAST of Chy12G219560 vs. ExPASy Swiss-Prot
Match: Q9FTA0 (GEM-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=At5g08350 PE=2 SV=1)

HSP 1 Score: 188.0 bits (476), Expect = 1.2e-46
Identity = 95/180 (52.78%), Postives = 128/180 (71.11%), Query Frame = 0

Query: 31  PSSSGNDNSKSGRNKQFIRKRGGFVFRVYEHVKLGPKFLVTAKGKLRLGAKIIQQGGRKN 90
           P+SS      + ++    +K  GF   V +  K+ PK   T K KL LGA+I+Q GG + 
Sbjct: 36  PASSKKSEQSNVKSILKRKKTDGFTNGVRDQSKIRPKLTETVKRKLSLGARILQVGGLEK 95

Query: 91  IFKQVFGIVEGEQLLKASQCYLSTSAGPIAGLLFISTEKVAFCSEQSITFSSPTGELLKT 150
           IFK++F + EGE+L K  QCYLST+AGPIAGLLFIS++K+AFCSE+SI   SP G++++ 
Sbjct: 96  IFKRLFRVSEGEKLFKMYQCYLSTTAGPIAGLLFISSKKMAFCSERSIKVDSPQGDIIRV 155

Query: 151 PYKVLIPLKKIRKANQSENVNDPAKKYIEVVTDDNFDFWFMGFLRYEKAFTNLQKAISMA 210
            YKV IPL KI + NQS+N   P++KY+EVVT D FDFWFMGFL Y+KAF  L+KA+S++
Sbjct: 156 HYKVSIPLCKIDRVNQSQNTKKPSQKYLEVVTVDGFDFWFMGFLSYQKAFNCLEKALSLS 215

BLAST of Chy12G219560 vs. ExPASy Swiss-Prot
Match: Q9FMW6 (GEM-like protein 6 OS=Arabidopsis thaliana OX=3702 GN=At5g23350 PE=2 SV=2)

HSP 1 Score: 187.2 bits (474), Expect = 2.0e-46
Identity = 93/170 (54.71%), Postives = 123/170 (72.35%), Query Frame = 0

Query: 41  SGRNKQFIRKR--GGFVFRVYEHVKLGPKFLVTAKGKLRLGAKIIQQGGRKNIFKQVFGI 100
           +G+ K  +RK+    F     +  KLGPK   T K KL LGAKI+Q GG + I+K++F +
Sbjct: 45  TGKGKSMLRKKKTDSFTNGARDQDKLGPKLTETVKRKLSLGAKILQMGGLEKIYKRLFKV 104

Query: 101 VEGEQLLKASQCYLSTSAGPIAGLLFISTEKVAFCSEQSITFSSPTGELLKTPYKVLIPL 160
            + E+L KA QCYLST+AGPIAGLLFIS++K+AFCSE+SI  +SP G L +  YKV IPL
Sbjct: 105 CDQEKLFKAYQCYLSTTAGPIAGLLFISSKKIAFCSERSIKVASPQGVLSRVHYKVSIPL 164

Query: 161 KKIRKANQSENVNDPAKKYIEVVTDDNFDFWFMGFLRYEKAFTNLQKAIS 209
            KI   NQS+N   P++KY+E+VT DNFDFWFMGF+ Y+KAF  L+KA++
Sbjct: 165 CKINGVNQSQNTKKPSQKYLEIVTIDNFDFWFMGFVSYQKAFNCLEKALN 214

BLAST of Chy12G219560 vs. ExPASy Swiss-Prot
Match: Q9FMW5 (GEM-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=At5g23360 PE=2 SV=1)

HSP 1 Score: 179.1 bits (453), Expect = 5.5e-44
Identity = 87/160 (54.37%), Postives = 116/160 (72.50%), Query Frame = 0

Query: 49  RKRGGFVFRVYEHVKLGPKFLVTAKGKLRLGAKIIQQGGRKNIFKQVFGIVEGEQLLKAS 108
           +K   F     +  KLGPK   T K KL LGAKI+Q GG + I+K++F + + E+L KA 
Sbjct: 47  KKTDSFTNGARDQDKLGPKLTETVKRKLSLGAKILQMGGLEKIYKRLFKVCDKEKLFKAY 106

Query: 109 QCYLSTSAGPIAGLLFISTEKVAFCSEQSITFSSPTGELLKTPYKVLIPLKKIRKANQSE 168
           QCYLST+ G IAGLLFIS++K+AFCSE+SI  +SP G+L +  YKV IPL KI   NQS+
Sbjct: 107 QCYLSTTEGSIAGLLFISSKKIAFCSERSIKVTSPQGDLTRVHYKVSIPLCKINGVNQSQ 166

Query: 169 NVNDPAKKYIEVVTDDNFDFWFMGFLRYEKAFTNLQKAIS 209
           N   P+++Y+EVVT DN+DFWFMGF+ Y+KAF  L+KA++
Sbjct: 167 NTKKPSQRYLEVVTVDNYDFWFMGFVSYQKAFNCLEKALN 206

BLAST of Chy12G219560 vs. ExPASy Swiss-Prot
Match: Q9LYV6 (GEM-like protein 5 OS=Arabidopsis thaliana OX=3702 GN=At5g13200 PE=1 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 2.6e-30
Identity = 64/160 (40.00%), Postives = 100/160 (62.50%), Query Frame = 0

Query: 49  RKRGGFVFRVYEHVKLGPKFLVTAKGKLRLGAKIIQQGGRKNIFKQVFGIVEGEQLLKAS 108
           RK       ++ ++K GP    TA GK+ L AK I +GG +++F+Q+FG    E L K  
Sbjct: 102 RKAETVARNLWHNLKTGPSMSETAWGKVNLTAKAITKGGFESLFRQIFGTEPNETLKKTF 161

Query: 109 QCYLSTSAGPIAGLLFISTEKVAFCSEQSITFSSPTGELLKTPYKVLIPLKKIRKANQSE 168
            CYLST+ GP+AG +++S  +VAFCS++ + F++P+G+   + Y+V++PL  +   N   
Sbjct: 162 ACYLSTTTGPVAGTVYLSNARVAFCSDRPLYFTAPSGQESWSYYRVVVPLANVATVNPVV 221

Query: 169 NVNDPAKKYIEVVTDDNFDFWFMGFLRYEKAFTNLQKAIS 209
               P +KYI++ T D  DFWFMGF+ YEKA  +L  ++S
Sbjct: 222 VKETPPEKYIQLTTVDGHDFWFMGFVNYEKATHHLLTSVS 261

BLAST of Chy12G219560 vs. ExPASy TrEMBL
Match: A0A0A0LW01 (GRAM domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G043190 PE=3 SV=1)

HSP 1 Score: 424.1 bits (1089), Expect = 3.6e-115
Identity = 217/221 (98.19%), Postives = 218/221 (98.64%), Query Frame = 0

Query: 1   MKSFDKTPDRNSSFSEPVSSIHSPLYSDGSPSSSGNDNSKSGRNKQFIRKRGGFVFRVYE 60
           MKSFDKTPDRNSSFSEPVSSIHSPLYSDGSPSSSGN+NSKSGR KQ IRKRGGFVFRVYE
Sbjct: 2   MKSFDKTPDRNSSFSEPVSSIHSPLYSDGSPSSSGNNNSKSGRKKQLIRKRGGFVFRVYE 61

Query: 61  HVKLGPKFLVTAKGKLRLGAKIIQQGGRKNIFKQVFGIVEGEQLLKASQCYLSTSAGPIA 120
           HVKLGPKFLVTAKGKLRLGAKIIQQGGRKNIFKQVFGIVEGEQLLKASQCYLSTSAGPIA
Sbjct: 62  HVKLGPKFLVTAKGKLRLGAKIIQQGGRKNIFKQVFGIVEGEQLLKASQCYLSTSAGPIA 121

Query: 121 GLLFISTEKVAFCSEQSITFSSPTGELLKTPYKVLIPLKKIRKANQSENVNDPAKKYIEV 180
           GLLFISTEKVAFCSEQSITFSSPTGELLKTPYKVLIPLKKIRKANQSENVNDPAKKYIEV
Sbjct: 122 GLLFISTEKVAFCSEQSITFSSPTGELLKTPYKVLIPLKKIRKANQSENVNDPAKKYIEV 181

Query: 181 VTDDNFDFWFMGFLRYEKAFTNLQKAISMANNSMQQSTDIS 222
           VTDDNFDFWFMGFLRYEKAFTNLQKAISMANNSMQQ TDIS
Sbjct: 182 VTDDNFDFWFMGFLRYEKAFTNLQKAISMANNSMQQFTDIS 222

BLAST of Chy12G219560 vs. ExPASy TrEMBL
Match: A0A5D3BH38 (GEM-like protein 4 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G002760 PE=3 SV=1)

HSP 1 Score: 423.3 bits (1087), Expect = 6.2e-115
Identity = 216/221 (97.74%), Postives = 217/221 (98.19%), Query Frame = 0

Query: 1   MKSFDKTPDRNSSFSEPVSSIHSPLYSDGSPSSSGNDNSKSGRNKQFIRKRGGFVFRVYE 60
           MKSFDKTPDRNSSFSEPVSSIHSPLYSDGSPSSSGNDNSKSG  KQFIRKRGGFVFRVYE
Sbjct: 64  MKSFDKTPDRNSSFSEPVSSIHSPLYSDGSPSSSGNDNSKSGHKKQFIRKRGGFVFRVYE 123

Query: 61  HVKLGPKFLVTAKGKLRLGAKIIQQGGRKNIFKQVFGIVEGEQLLKASQCYLSTSAGPIA 120
           HVKLGPKFLVTAKGKLRLGAKII+QGGRKNIFKQVFGIVEGEQLLKASQCYLSTSAGPIA
Sbjct: 124 HVKLGPKFLVTAKGKLRLGAKIIRQGGRKNIFKQVFGIVEGEQLLKASQCYLSTSAGPIA 183

Query: 121 GLLFISTEKVAFCSEQSITFSSPTGELLKTPYKVLIPLKKIRKANQSENVNDPAKKYIEV 180
           GLLFISTEKVAFCSEQSITFSSPTGELLKTPYKVLIPLKKIRKANQSENVNDP KKYIEV
Sbjct: 184 GLLFISTEKVAFCSEQSITFSSPTGELLKTPYKVLIPLKKIRKANQSENVNDPTKKYIEV 243

Query: 181 VTDDNFDFWFMGFLRYEKAFTNLQKAISMANNSMQQSTDIS 222
           VTDDNFDFWFMGFLRYEKAFTNLQKAISMAN SMQQSTDIS
Sbjct: 244 VTDDNFDFWFMGFLRYEKAFTNLQKAISMANRSMQQSTDIS 284

BLAST of Chy12G219560 vs. ExPASy TrEMBL
Match: A0A1S3AT09 (GEM-like protein 4 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103482572 PE=3 SV=1)

HSP 1 Score: 423.3 bits (1087), Expect = 6.2e-115
Identity = 216/221 (97.74%), Postives = 217/221 (98.19%), Query Frame = 0

Query: 1   MKSFDKTPDRNSSFSEPVSSIHSPLYSDGSPSSSGNDNSKSGRNKQFIRKRGGFVFRVYE 60
           MKSFDKTPDRNSSFSEPVSSIHSPLYSDGSPSSSGNDNSKSG  KQFIRKRGGFVFRVYE
Sbjct: 1   MKSFDKTPDRNSSFSEPVSSIHSPLYSDGSPSSSGNDNSKSGHKKQFIRKRGGFVFRVYE 60

Query: 61  HVKLGPKFLVTAKGKLRLGAKIIQQGGRKNIFKQVFGIVEGEQLLKASQCYLSTSAGPIA 120
           HVKLGPKFLVTAKGKLRLGAKII+QGGRKNIFKQVFGIVEGEQLLKASQCYLSTSAGPIA
Sbjct: 61  HVKLGPKFLVTAKGKLRLGAKIIRQGGRKNIFKQVFGIVEGEQLLKASQCYLSTSAGPIA 120

Query: 121 GLLFISTEKVAFCSEQSITFSSPTGELLKTPYKVLIPLKKIRKANQSENVNDPAKKYIEV 180
           GLLFISTEKVAFCSEQSITFSSPTGELLKTPYKVLIPLKKIRKANQSENVNDP KKYIEV
Sbjct: 121 GLLFISTEKVAFCSEQSITFSSPTGELLKTPYKVLIPLKKIRKANQSENVNDPTKKYIEV 180

Query: 181 VTDDNFDFWFMGFLRYEKAFTNLQKAISMANNSMQQSTDIS 222
           VTDDNFDFWFMGFLRYEKAFTNLQKAISMAN SMQQSTDIS
Sbjct: 181 VTDDNFDFWFMGFLRYEKAFTNLQKAISMANRSMQQSTDIS 221

BLAST of Chy12G219560 vs. ExPASy TrEMBL
Match: A0A1S3ATK3 (GEM-like protein 4 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103482572 PE=3 SV=1)

HSP 1 Score: 391.3 bits (1004), Expect = 2.6e-105
Identity = 204/221 (92.31%), Postives = 205/221 (92.76%), Query Frame = 0

Query: 1   MKSFDKTPDRNSSFSEPVSSIHSPLYSDGSPSSSGNDNSKSGRNKQFIRKRGGFVFRVYE 60
           MKSFDKTPDRNSSFSEPVSSIHSPLYSDGSPSSSGNDNSKSG  KQFIRKRGGFVFRVYE
Sbjct: 1   MKSFDKTPDRNSSFSEPVSSIHSPLYSDGSPSSSGNDNSKSGHKKQFIRKRGGFVFRVYE 60

Query: 61  HVKLGPKFLVTAKGKLRLGAKIIQQGGRKNIFKQVFGIVEGEQLLKASQCYLSTSAGPIA 120
           H            GKLRLGAKII+QGGRKNIFKQVFGIVEGEQLLKASQCYLSTSAGPIA
Sbjct: 61  H------------GKLRLGAKIIRQGGRKNIFKQVFGIVEGEQLLKASQCYLSTSAGPIA 120

Query: 121 GLLFISTEKVAFCSEQSITFSSPTGELLKTPYKVLIPLKKIRKANQSENVNDPAKKYIEV 180
           GLLFISTEKVAFCSEQSITFSSPTGELLKTPYKVLIPLKKIRKANQSENVNDP KKYIEV
Sbjct: 121 GLLFISTEKVAFCSEQSITFSSPTGELLKTPYKVLIPLKKIRKANQSENVNDPTKKYIEV 180

Query: 181 VTDDNFDFWFMGFLRYEKAFTNLQKAISMANNSMQQSTDIS 222
           VTDDNFDFWFMGFLRYEKAFTNLQKAISMAN SMQQSTDIS
Sbjct: 181 VTDDNFDFWFMGFLRYEKAFTNLQKAISMANRSMQQSTDIS 209

BLAST of Chy12G219560 vs. ExPASy TrEMBL
Match: A0A6J1C718 (GEM-like protein 4 OS=Momordica charantia OX=3673 GN=LOC111008582 PE=3 SV=1)

HSP 1 Score: 353.6 bits (906), Expect = 6.0e-94
Identity = 177/211 (83.89%), Postives = 193/211 (91.47%), Query Frame = 0

Query: 1   MKSFDKTPDRNSSFSEPVSSIHSPLYSDGSPSSSGNDNSKSGRNKQFIRKRGGFVFRVYE 60
           MKSFD TP+R SSFSEPVSSI+SP YSDGSP SSG + SK G  KQ +RKRGGFVFR+Y+
Sbjct: 1   MKSFDTTPERRSSFSEPVSSINSPFYSDGSPYSSGKEISKLGHKKQLVRKRGGFVFRIYD 60

Query: 61  HVKLGPKFLVTAKGKLRLGAKIIQQGGRKNIFKQVFGIVEGEQLLKASQCYLSTSAGPIA 120
           HVKLGPKF VTAKGKLRLGAKIIQQGG KNI KQVFGI+EGE+LLKASQCYLSTS GPIA
Sbjct: 61  HVKLGPKFSVTAKGKLRLGAKIIQQGGIKNILKQVFGILEGEKLLKASQCYLSTSVGPIA 120

Query: 121 GLLFISTEKVAFCSEQSITFSSPTGELLKTPYKVLIPLKKIRKANQSENVNDPAKKYIEV 180
           GLLFISTEKVAFCS+Q ITFSSPTGELLKTPYKVLIPLK IRK ++SEN ++PAKKYIEV
Sbjct: 121 GLLFISTEKVAFCSDQRITFSSPTGELLKTPYKVLIPLKNIRKVSRSENADEPAKKYIEV 180

Query: 181 VTDDNFDFWFMGFLRYEKAFTNLQKAISMAN 212
           +T+D+FDFWFMGFLRYEKAFTNLQKAISMAN
Sbjct: 181 ITEDDFDFWFMGFLRYEKAFTNLQKAISMAN 211

BLAST of Chy12G219560 vs. NCBI nr
Match: XP_004137532.1 (putative GEM-like protein 8 [Cucumis sativus] >KGN64216.1 hypothetical protein Csa_013226 [Cucumis sativus])

HSP 1 Score: 423 bits (1087), Expect = 7.04e-149
Identity = 217/221 (98.19%), Postives = 218/221 (98.64%), Query Frame = 0

Query: 1   MKSFDKTPDRNSSFSEPVSSIHSPLYSDGSPSSSGNDNSKSGRNKQFIRKRGGFVFRVYE 60
           MKSFDKTPDRNSSFSEPVSSIHSPLYSDGSPSSSGN+NSKSGR KQ IRKRGGFVFRVYE
Sbjct: 2   MKSFDKTPDRNSSFSEPVSSIHSPLYSDGSPSSSGNNNSKSGRKKQLIRKRGGFVFRVYE 61

Query: 61  HVKLGPKFLVTAKGKLRLGAKIIQQGGRKNIFKQVFGIVEGEQLLKASQCYLSTSAGPIA 120
           HVKLGPKFLVTAKGKLRLGAKIIQQGGRKNIFKQVFGIVEGEQLLKASQCYLSTSAGPIA
Sbjct: 62  HVKLGPKFLVTAKGKLRLGAKIIQQGGRKNIFKQVFGIVEGEQLLKASQCYLSTSAGPIA 121

Query: 121 GLLFISTEKVAFCSEQSITFSSPTGELLKTPYKVLIPLKKIRKANQSENVNDPAKKYIEV 180
           GLLFISTEKVAFCSEQSITFSSPTGELLKTPYKVLIPLKKIRKANQSENVNDPAKKYIEV
Sbjct: 122 GLLFISTEKVAFCSEQSITFSSPTGELLKTPYKVLIPLKKIRKANQSENVNDPAKKYIEV 181

Query: 181 VTDDNFDFWFMGFLRYEKAFTNLQKAISMANNSMQQSTDIS 221
           VTDDNFDFWFMGFLRYEKAFTNLQKAISMANNSMQQ TDIS
Sbjct: 182 VTDDNFDFWFMGFLRYEKAFTNLQKAISMANNSMQQFTDIS 222

BLAST of Chy12G219560 vs. NCBI nr
Match: XP_008437022.1 (PREDICTED: GEM-like protein 4 isoform X1 [Cucumis melo])

HSP 1 Score: 422 bits (1085), Expect = 1.37e-148
Identity = 216/221 (97.74%), Postives = 217/221 (98.19%), Query Frame = 0

Query: 1   MKSFDKTPDRNSSFSEPVSSIHSPLYSDGSPSSSGNDNSKSGRNKQFIRKRGGFVFRVYE 60
           MKSFDKTPDRNSSFSEPVSSIHSPLYSDGSPSSSGNDNSKSG  KQFIRKRGGFVFRVYE
Sbjct: 1   MKSFDKTPDRNSSFSEPVSSIHSPLYSDGSPSSSGNDNSKSGHKKQFIRKRGGFVFRVYE 60

Query: 61  HVKLGPKFLVTAKGKLRLGAKIIQQGGRKNIFKQVFGIVEGEQLLKASQCYLSTSAGPIA 120
           HVKLGPKFLVTAKGKLRLGAKII+QGGRKNIFKQVFGIVEGEQLLKASQCYLSTSAGPIA
Sbjct: 61  HVKLGPKFLVTAKGKLRLGAKIIRQGGRKNIFKQVFGIVEGEQLLKASQCYLSTSAGPIA 120

Query: 121 GLLFISTEKVAFCSEQSITFSSPTGELLKTPYKVLIPLKKIRKANQSENVNDPAKKYIEV 180
           GLLFISTEKVAFCSEQSITFSSPTGELLKTPYKVLIPLKKIRKANQSENVNDP KKYIEV
Sbjct: 121 GLLFISTEKVAFCSEQSITFSSPTGELLKTPYKVLIPLKKIRKANQSENVNDPTKKYIEV 180

Query: 181 VTDDNFDFWFMGFLRYEKAFTNLQKAISMANNSMQQSTDIS 221
           VTDDNFDFWFMGFLRYEKAFTNLQKAISMAN SMQQSTDIS
Sbjct: 181 VTDDNFDFWFMGFLRYEKAFTNLQKAISMANRSMQQSTDIS 221

BLAST of Chy12G219560 vs. NCBI nr
Match: TYJ99072.1 (GEM-like protein 4 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 422 bits (1085), Expect = 1.32e-147
Identity = 216/221 (97.74%), Postives = 217/221 (98.19%), Query Frame = 0

Query: 1   MKSFDKTPDRNSSFSEPVSSIHSPLYSDGSPSSSGNDNSKSGRNKQFIRKRGGFVFRVYE 60
           MKSFDKTPDRNSSFSEPVSSIHSPLYSDGSPSSSGNDNSKSG  KQFIRKRGGFVFRVYE
Sbjct: 64  MKSFDKTPDRNSSFSEPVSSIHSPLYSDGSPSSSGNDNSKSGHKKQFIRKRGGFVFRVYE 123

Query: 61  HVKLGPKFLVTAKGKLRLGAKIIQQGGRKNIFKQVFGIVEGEQLLKASQCYLSTSAGPIA 120
           HVKLGPKFLVTAKGKLRLGAKII+QGGRKNIFKQVFGIVEGEQLLKASQCYLSTSAGPIA
Sbjct: 124 HVKLGPKFLVTAKGKLRLGAKIIRQGGRKNIFKQVFGIVEGEQLLKASQCYLSTSAGPIA 183

Query: 121 GLLFISTEKVAFCSEQSITFSSPTGELLKTPYKVLIPLKKIRKANQSENVNDPAKKYIEV 180
           GLLFISTEKVAFCSEQSITFSSPTGELLKTPYKVLIPLKKIRKANQSENVNDP KKYIEV
Sbjct: 184 GLLFISTEKVAFCSEQSITFSSPTGELLKTPYKVLIPLKKIRKANQSENVNDPTKKYIEV 243

Query: 181 VTDDNFDFWFMGFLRYEKAFTNLQKAISMANNSMQQSTDIS 221
           VTDDNFDFWFMGFLRYEKAFTNLQKAISMAN SMQQSTDIS
Sbjct: 244 VTDDNFDFWFMGFLRYEKAFTNLQKAISMANRSMQQSTDIS 284

BLAST of Chy12G219560 vs. NCBI nr
Match: XP_008437030.1 (PREDICTED: GEM-like protein 4 isoform X2 [Cucumis melo])

HSP 1 Score: 390 bits (1001), Expect = 5.69e-136
Identity = 204/221 (92.31%), Postives = 205/221 (92.76%), Query Frame = 0

Query: 1   MKSFDKTPDRNSSFSEPVSSIHSPLYSDGSPSSSGNDNSKSGRNKQFIRKRGGFVFRVYE 60
           MKSFDKTPDRNSSFSEPVSSIHSPLYSDGSPSSSGNDNSKSG  KQFIRKRGGFVFRVYE
Sbjct: 1   MKSFDKTPDRNSSFSEPVSSIHSPLYSDGSPSSSGNDNSKSGHKKQFIRKRGGFVFRVYE 60

Query: 61  HVKLGPKFLVTAKGKLRLGAKIIQQGGRKNIFKQVFGIVEGEQLLKASQCYLSTSAGPIA 120
           H            GKLRLGAKII+QGGRKNIFKQVFGIVEGEQLLKASQCYLSTSAGPIA
Sbjct: 61  H------------GKLRLGAKIIRQGGRKNIFKQVFGIVEGEQLLKASQCYLSTSAGPIA 120

Query: 121 GLLFISTEKVAFCSEQSITFSSPTGELLKTPYKVLIPLKKIRKANQSENVNDPAKKYIEV 180
           GLLFISTEKVAFCSEQSITFSSPTGELLKTPYKVLIPLKKIRKANQSENVNDP KKYIEV
Sbjct: 121 GLLFISTEKVAFCSEQSITFSSPTGELLKTPYKVLIPLKKIRKANQSENVNDPTKKYIEV 180

Query: 181 VTDDNFDFWFMGFLRYEKAFTNLQKAISMANNSMQQSTDIS 221
           VTDDNFDFWFMGFLRYEKAFTNLQKAISMAN SMQQSTDIS
Sbjct: 181 VTDDNFDFWFMGFLRYEKAFTNLQKAISMANRSMQQSTDIS 209

BLAST of Chy12G219560 vs. NCBI nr
Match: XP_038895201.1 (GEM-like protein 4 isoform X1 [Benincasa hispida])

HSP 1 Score: 386 bits (992), Expect = 1.60e-134
Identity = 198/211 (93.84%), Postives = 202/211 (95.73%), Query Frame = 0

Query: 1   MKSFDKTPDRNSSFSEPVSSIHSPLYSDGSPSSSGNDNSKSGRNKQFIRKRGGFVFRVYE 60
           MKSFDKTPDRNSSFSEPVSSI SPLYSDGSPSSSGND+ K G  KQFIRKRGGFVFRV +
Sbjct: 1   MKSFDKTPDRNSSFSEPVSSIQSPLYSDGSPSSSGNDHPKLGHKKQFIRKRGGFVFRVLD 60

Query: 61  HVKLGPKFLVTAKGKLRLGAKIIQQGGRKNIFKQVFGIVEGEQLLKASQCYLSTSAGPIA 120
           HVKLGPKFLVTAKGKLRLGAKIIQQGGRKNIFKQVFGIVEGEQLLKASQCYLSTSAGPIA
Sbjct: 61  HVKLGPKFLVTAKGKLRLGAKIIQQGGRKNIFKQVFGIVEGEQLLKASQCYLSTSAGPIA 120

Query: 121 GLLFISTEKVAFCSEQSITFSSPTGELLKTPYKVLIPLKKIRKANQSENVNDPAKKYIEV 180
           GLLF+STEKVAFCSEQSIT SSPTGELLKT YKV IPLKKIRKANQSENVN+PAKKYIEV
Sbjct: 121 GLLFVSTEKVAFCSEQSITLSSPTGELLKTHYKVSIPLKKIRKANQSENVNEPAKKYIEV 180

Query: 181 VTDDNFDFWFMGFLRYEKAFTNLQKAISMAN 211
           VTDDNFDFWFMGFLRYEKAFTNLQKAISMAN
Sbjct: 181 VTDDNFDFWFMGFLRYEKAFTNLQKAISMAN 211

BLAST of Chy12G219560 vs. TAIR 10
Match: AT5G23370.1 (GRAM domain-containing protein / ABA-responsive protein-related )

HSP 1 Score: 189.5 bits (480), Expect = 2.9e-48
Identity = 95/172 (55.23%), Postives = 126/172 (73.26%), Query Frame = 0

Query: 41  SGRNKQFIRKR--GGFVFRVYEHVKLGPKFLVTAKGKLRLGAKIIQQGGRKNIFKQVFGI 100
           +G+ K  +RK+    F   V +  KLGPK   T K KL LGA+I+Q GG + I+K++F +
Sbjct: 45  TGKGKSMLRKKKNDSFTNGVRDQDKLGPKLTETVKRKLSLGARILQMGGLEKIYKRLFKV 104

Query: 101 VEGEQLLKASQCYLSTSAGPIAGLLFISTEKVAFCSEQSITFSSPTGELLKTPYKVLIPL 160
            + E+L KA QCYLST+AGPIAGLLFIS++K+AFCSE+SI  +SP GEL +  YKV IPL
Sbjct: 105 SDEEKLFKAYQCYLSTTAGPIAGLLFISSKKIAFCSERSIKVASPQGELNRVHYKVSIPL 164

Query: 161 KKIRKANQSENVNDPAKKYIEVVTDDNFDFWFMGFLRYEKAFTNLQKAISMA 211
            KI   NQS+N   P++KY+EVVT D FDFWFMGFL Y+KAF  L++A+S++
Sbjct: 165 CKINGVNQSQNTTKPSQKYLEVVTVDGFDFWFMGFLSYQKAFNCLEQALSLS 216

BLAST of Chy12G219560 vs. TAIR 10
Match: AT5G08350.1 (GRAM domain-containing protein / ABA-responsive protein-related )

HSP 1 Score: 188.0 bits (476), Expect = 8.4e-48
Identity = 95/180 (52.78%), Postives = 128/180 (71.11%), Query Frame = 0

Query: 31  PSSSGNDNSKSGRNKQFIRKRGGFVFRVYEHVKLGPKFLVTAKGKLRLGAKIIQQGGRKN 90
           P+SS      + ++    +K  GF   V +  K+ PK   T K KL LGA+I+Q GG + 
Sbjct: 36  PASSKKSEQSNVKSILKRKKTDGFTNGVRDQSKIRPKLTETVKRKLSLGARILQVGGLEK 95

Query: 91  IFKQVFGIVEGEQLLKASQCYLSTSAGPIAGLLFISTEKVAFCSEQSITFSSPTGELLKT 150
           IFK++F + EGE+L K  QCYLST+AGPIAGLLFIS++K+AFCSE+SI   SP G++++ 
Sbjct: 96  IFKRLFRVSEGEKLFKMYQCYLSTTAGPIAGLLFISSKKMAFCSERSIKVDSPQGDIIRV 155

Query: 151 PYKVLIPLKKIRKANQSENVNDPAKKYIEVVTDDNFDFWFMGFLRYEKAFTNLQKAISMA 210
            YKV IPL KI + NQS+N   P++KY+EVVT D FDFWFMGFL Y+KAF  L+KA+S++
Sbjct: 156 HYKVSIPLCKIDRVNQSQNTKKPSQKYLEVVTVDGFDFWFMGFLSYQKAFNCLEKALSLS 215

BLAST of Chy12G219560 vs. TAIR 10
Match: AT5G23350.1 (GRAM domain-containing protein / ABA-responsive protein-related )

HSP 1 Score: 187.2 bits (474), Expect = 1.4e-47
Identity = 93/170 (54.71%), Postives = 123/170 (72.35%), Query Frame = 0

Query: 41  SGRNKQFIRKR--GGFVFRVYEHVKLGPKFLVTAKGKLRLGAKIIQQGGRKNIFKQVFGI 100
           +G+ K  +RK+    F     +  KLGPK   T K KL LGAKI+Q GG + I+K++F +
Sbjct: 107 TGKGKSMLRKKKTDSFTNGARDQDKLGPKLTETVKRKLSLGAKILQMGGLEKIYKRLFKV 166

Query: 101 VEGEQLLKASQCYLSTSAGPIAGLLFISTEKVAFCSEQSITFSSPTGELLKTPYKVLIPL 160
            + E+L KA QCYLST+AGPIAGLLFIS++K+AFCSE+SI  +SP G L +  YKV IPL
Sbjct: 167 CDQEKLFKAYQCYLSTTAGPIAGLLFISSKKIAFCSERSIKVASPQGVLSRVHYKVSIPL 226

Query: 161 KKIRKANQSENVNDPAKKYIEVVTDDNFDFWFMGFLRYEKAFTNLQKAIS 209
            KI   NQS+N   P++KY+E+VT DNFDFWFMGF+ Y+KAF  L+KA++
Sbjct: 227 CKINGVNQSQNTKKPSQKYLEIVTIDNFDFWFMGFVSYQKAFNCLEKALN 276

BLAST of Chy12G219560 vs. TAIR 10
Match: AT5G23360.1 (GRAM domain-containing protein / ABA-responsive protein-related )

HSP 1 Score: 179.1 bits (453), Expect = 3.9e-45
Identity = 87/160 (54.37%), Postives = 116/160 (72.50%), Query Frame = 0

Query: 49  RKRGGFVFRVYEHVKLGPKFLVTAKGKLRLGAKIIQQGGRKNIFKQVFGIVEGEQLLKAS 108
           +K   F     +  KLGPK   T K KL LGAKI+Q GG + I+K++F + + E+L KA 
Sbjct: 47  KKTDSFTNGARDQDKLGPKLTETVKRKLSLGAKILQMGGLEKIYKRLFKVCDKEKLFKAY 106

Query: 109 QCYLSTSAGPIAGLLFISTEKVAFCSEQSITFSSPTGELLKTPYKVLIPLKKIRKANQSE 168
           QCYLST+ G IAGLLFIS++K+AFCSE+SI  +SP G+L +  YKV IPL KI   NQS+
Sbjct: 107 QCYLSTTEGSIAGLLFISSKKIAFCSERSIKVTSPQGDLTRVHYKVSIPLCKINGVNQSQ 166

Query: 169 NVNDPAKKYIEVVTDDNFDFWFMGFLRYEKAFTNLQKAIS 209
           N   P+++Y+EVVT DN+DFWFMGF+ Y+KAF  L+KA++
Sbjct: 167 NTKKPSQRYLEVVTVDNYDFWFMGFVSYQKAFNCLEKALN 206

BLAST of Chy12G219560 vs. TAIR 10
Match: AT2G22475.1 (GRAM domain family protein )

HSP 1 Score: 133.7 bits (335), Expect = 1.9e-31
Identity = 63/160 (39.38%), Postives = 105/160 (65.62%), Query Frame = 0

Query: 49  RKRGGFVFRVYEHVKLGPKFLVTAKGKLRLGAKIIQQGGRKNIFKQVFGIVEGEQLLKAS 108
           +K        ++H++  P F   A G++    K+  +GG + IF+Q F     EQLL + 
Sbjct: 134 KKTESLAGNTWQHLRTAPSFADAAMGRIAQSTKVFAEGGYEKIFRQTFETDPEEQLLNSF 193

Query: 109 QCYLSTSAGPIAGLLFISTEKVAFCSEQSITFSSPTGELLK-TPYKVLIPLKKIRKANQS 168
            CYLSTSAGP+ G+L+IS+ K+A+CS+  +++ +  G+  + + YKV+IPL +++  N S
Sbjct: 194 ACYLSTSAGPVMGVLYISSAKLAYCSDNPLSYKN--GDQTEWSYYKVVIPLHQLKAVNPS 253

Query: 169 ENVNDPAKKYIEVVTDDNFDFWFMGFLRYEKAFTNLQKAI 208
            ++ +PA+KYI+V++ DN +FWFMGFL Y+ A T+LQ ++
Sbjct: 254 ASIVNPAEKYIQVISVDNHEFWFMGFLNYDGAVTSLQDSL 291

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FMW44.1e-4755.23Putative GEM-like protein 8 OS=Arabidopsis thaliana OX=3702 GN=At5g23370 PE=3 SV... [more]
Q9FTA01.2e-4652.78GEM-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=At5g08350 PE=2 SV=1[more]
Q9FMW62.0e-4654.71GEM-like protein 6 OS=Arabidopsis thaliana OX=3702 GN=At5g23350 PE=2 SV=2[more]
Q9FMW55.5e-4454.38GEM-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=At5g23360 PE=2 SV=1[more]
Q9LYV62.6e-3040.00GEM-like protein 5 OS=Arabidopsis thaliana OX=3702 GN=At5g13200 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LW013.6e-11598.19GRAM domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G043190 PE=3 S... [more]
A0A5D3BH386.2e-11597.74GEM-like protein 4 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc... [more]
A0A1S3AT096.2e-11597.74GEM-like protein 4 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103482572 PE=3 SV=1[more]
A0A1S3ATK32.6e-10592.31GEM-like protein 4 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103482572 PE=3 SV=1[more]
A0A6J1C7186.0e-9483.89GEM-like protein 4 OS=Momordica charantia OX=3673 GN=LOC111008582 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_004137532.17.04e-14998.19putative GEM-like protein 8 [Cucumis sativus] >KGN64216.1 hypothetical protein C... [more]
XP_008437022.11.37e-14897.74PREDICTED: GEM-like protein 4 isoform X1 [Cucumis melo][more]
TYJ99072.11.32e-14797.74GEM-like protein 4 isoform X1 [Cucumis melo var. makuwa][more]
XP_008437030.15.69e-13692.31PREDICTED: GEM-like protein 4 isoform X2 [Cucumis melo][more]
XP_038895201.11.60e-13493.84GEM-like protein 4 isoform X1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT5G23370.12.9e-4855.23GRAM domain-containing protein / ABA-responsive protein-related [more]
AT5G08350.18.4e-4852.78GRAM domain-containing protein / ABA-responsive protein-related [more]
AT5G23350.11.4e-4754.71GRAM domain-containing protein / ABA-responsive protein-related [more]
AT5G23360.13.9e-4554.38GRAM domain-containing protein / ABA-responsive protein-related [more]
AT2G22475.11.9e-3139.38GRAM domain family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004182GRAM domainSMARTSM00568gram2001ccoord: 90..168
e-value: 1.6E-12
score: 57.6
IPR004182GRAM domainPFAMPF02893GRAMcoord: 91..211
e-value: 1.0E-16
score: 60.8
IPR011993PH-like domain superfamilyGENE3D2.30.29.30coord: 88..209
e-value: 5.7E-20
score: 73.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 10..42
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..44
NoneNo IPR availablePANTHERPTHR31969:SF1OSJNBA0038O10.18 PROTEINcoord: 13..208
IPR037848GEM-like proteinPANTHERPTHR31969GEM-LIKE PROTEIN 2coord: 13..208

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy12G219560.1Chy12G219560.1mRNA