Chy12G219550 (gene) Cucumber (hystrix) v1

Overview


Name	Chy12G219550
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	Kunitz inhibitor ST1-like protein
Location	chrH12: 17811645 .. 17817536 (+)
RNA-Seq Expression	Chy12G219550
Synteny	Chy12G219550

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAAAACTTTGCATTACTGTGTTTTCTTTTCATTGTCATTGCCTCATCTGAGGTACGCTTCTGCAGAGCGGACGCCTCCCCCGATGCGGTCCTCGACACCGACGGAAAGAAGCTTCGAGCCGGTGATCAATATTACATTCTCTCAGTTTACAACAGAAATAGCGGCGGATTAAGCATCGGCGGTATCTACGGATACGAAAAATGTCCCATCAACATCCTCCCCGAATCTTACGATTATTTACACGGTCTTCCGGCGACGTTTTCCCCCGTAAACCCTAAGAAAGGTGTGGTCCGAGTTTCTACCGATTTGAACATCCAATTCGAGGCGAACACGAGGTGTGGAATATCGACGGTGTGGAAAGTAGGTAAGTTTGATGAATACTTGAAGCAGTATTTTGTGATGATGGGCGGAACGAAAGGGAATCCGGGGTGGGAAACGATCGAAAATTGGTTCAAAGTGGAGAAGTATGGTAAGAATTATAAGTTGGTGTATTGTCCAACAGTTTGTAAGTATTGCAAAGTAATGTGCAAAGATGTGGGATTATTTTACAAGAATGGAAGGAGGGTTATTGCTTTGAATGATGCTCCATTCCCTGTTATGTTCAAGAAAGTTTGAATTTGAAGCTGATGATCATGATGAGAGGGAAATTAATGACTACATTTTCTCTCTCTATATCCAATGTAATAATAAATAAATTGTTGTAATCATCTTTATTGCAATAAAAGTTTATAGTTATTTTATTGAAAATTTGTTATAAATGACAAAAATTGAAGCTAATCATTCCTAAAATATCGCAAAATAGTCAATATTTTTACATTTATATGAATTTGATATTACCGCATTCATTATATCGTAAAAAATTCACAAAACACAAAACGTAATCATCAAAGTACTACTAATGTAAATAATAATAATTTTAAATAATAATCATGTTAAAGAAATCGTAAAATTAATAACTTCAAAATTTTCGTTAGCATCAACATTTTAAACCTTGACTAAAAACAACTATCATTGTTTAAAGAAGGTAAAGCTATTTATTGCTTTATTAAGGGTAGAAAATTATTATAAGTGACAAAATTGTTTGAAATATTTAAAAAATATAACAGAACTTTAAATTTATCAATTATATATAAACATTCAATGTTATAGATACTGCTAGAATACTATCAATGTTTGCATAAGTAATAAAATATTTATCCTCCATAGAAAAATGAAGTGTAACGAAGTTTTTGTCAATCAAATTTTTAATATTTTTTTTCTTAGCTGTTGATTTTTTTTTTATTATTATTATTTTAAAGAAAACCTGTATAATATCTTTATTAATCATTTGAGTTTTTGTTAGTTTGTGTTGGATGAAAATGTATAGAAAAGAAAGAGAAAAAGAAAAGTCCGTGTGTGTAAAATAGAAGTGTCAACATTTTCCAAGAGTCAAATACATACATACATCATGTGCAAAGCTAAAAGAGTTGTAAATGCAATCACATGAAAGCAATTAGTTTGACTTCAAGTAAAGATAATTTACTTTTTATTTTATTTTAAAATATATACTATTAGTTATTCTTGACGTTTTTTTTGACAGTTTTTATTAAAACTGTCAAGAATATCAAAACTGCAAGCGTTAAGTATTGTCAGTTTTATAGTAGTATATTAACTTTAAAATAAACTTAGCTCAACTTGATATTGGACAATAAAATATCTCGAATTCAAATCTTCTAATTTTTAATTTGTACTATTTTTTTTTCTCTCTTTTTTTTTGAAAATTTATCTTTACTAAGGAACTTAACGGACATGATAGGCTCAAAAAAGCTTTGACGGCCAATTAATTAAAATTTTATTGGCCCTTAGTCAAAAACTTGTTTTTGTATTCAATGTGTAGATCCCGGGGTTTCTTTACAAACTAATTATGATATAATTTTAGTTAAATACATGATTAATTACTTAATACCAATTTTATAATCAATAAAATTGCTTACTTTATTTTTCTTTCTTTAAAATTAAAAAAATTAACTAAATATATCAACTTTGAAATTATGTTTCACTTTGTTGAATTTTTCTTTTCTTTTTCCTCTTTGGATCCATTGTTTGACAGCTAGAAGCCATTAATTGGCACCACATAAGTCATAAACTACAATATAAAACTAGTAATAAAAACATATACAAAAAAGCCAAACAATAGTAGAATTAAACAAAGATAAAGTAAGTTAAAAAAAAATTAACAAGATCGATGAACTAATAAAAGGACTACACTATGGTTATGAAGAAATCTGCATTACTCACTTTTCTTTTCATTGCCATTGTCATTGCCTCATCTGAGTTACGCTTCTGCAGAGCTGATGCGTCACCGGATGCCGTCCTCGACACCGATGGAAAGAAGCTCAGAGTAAGCAACAAGTACTACATCCTCCAGCAATTCAAGGCAGCGGTGGTGGTGGAGGTTTAGCCATCGGCAATATCCGAAAAGAATACGATAGATGTGGTGTCAACGTCGTCCAGGAGCGTTACGAACAATTCGATGGTTTTCCCACGACATTTTTACCAAAGAACCTTAAAAAGGGTGTGGTTCGAGTCTCCACCGATTTGAACATCGAGTTTGATGGGACTACGAGGTGTGTGAAATCAACGGTGTGGAGATTAGGAACATTTGATCGATATTTGAGGCAATATTTCGTGACGATTGGCGGAACGAAAGGGAACCCGGGGCGAGAGACGACGGAAAATTGGTTCAAAATAGAGAAATATGGGAATGGTTACAAGTTGGTGTATTGTCCAAGAGTGGTGTGTAAGTATTGCAAAGTAATGTGCAAAGATATTGGTATATTCGAGAACAATGGGATAAGGGGTCTTGTTTTGAGCGATACGCCATTTCCCGTTATGTTCAAGAAAGTTTATGATTGATCAATGTGAAAGATGTCAAGAGGTCAGGTTTTGGGTGTGATCTGTGTTGAAATTTCAACCAATTCTCAATTTCTATTGTAATAATATATCACGACTTAATTTCATGAAATAAATAAAATATATCTCTAGTTATTATGTATGCTTTTATATATATATATAAACAATTGGATACTAGACATGGACCTAATAATAGGGTAAATTGCAAAAAAACACAACTGAAGTATGGTAATAATTGTAATTATATCAACAAAATTGGATGCTAAAACTATGTGTTGATTATATACGATTTTGAATTCAATTTTTATCATTTGTGGGTCCAACTTTTACAATTATTTGTAAGTATGAGGATCCAATTTTCACACTTTTAGAAGTTCAAAATTTCTTTATATTGCTATTGAAAGTTTGAGGTGTATTTGTAACTCGCACTTTGAGTACGAGCCTAACACTTTTGTTTTTGGTCTTTGATGAAAGATAAATTGCATCAAATGATAAAAATATTTTTAAAAAAACTCACTCATTGAGTGAAGGACTCTCTGTTGTGCAATTTACTTTATGTTGTCATGTGTCAAATTATATATAAAAAAATTCATCCTAATCATTGTCTAAAGATTTCATTTTCTCTTTAATTAATATTTGTTAGAGCCAGAATAATTGTGAGAAGTTTTGCTAATTTTTTAAGTTAGCAAATGAGAATATTTGGTTATAATAACCTTAATTCTTCTTCTTCTCGCAGTTCTATGCAGTTTCTTTAAATTCACTCAATTATTTTTTTAAAAAATAAAGAATATATGACCAAGCATTATTTTACAAATTCGCACAGACTTAAAAAAAATTATAAAATATATGATTTTATTCTCAAATTCAAACGTCAGTAATGAATTTTCAATCAAACCACTGTAAAAATCGATTATGGTCGGTTTAGTAAATGTTCAAACCGATCCGACTATAAAAGGGTACAATCTCATCAACCAATTGATGGGTTTAAAGCCTTTAGAATCTTTATCTAGCCGTCCATGATTTGGTTAGTGGCTCGATTTTTTTTGGTCAATCATATTTGTGAAGAATTCTATTTCAAAATATTGGATAAACTATTAAATATAAAACTTGTTTTTTATATATCATTGTGAAGTCATCTATCTCATCAGACTGGATAAGCTATTAAATATAAGATTGTTATCTCATTTTTCAAACTAATATGGTTTTTAAAAATTAGGGAAAAAGAAGTCGTCGGAAATTTAATTTATTTCAATAAACGTAGAGAAAAGGGAAATTAAAAGCCTTTGATTCACAGATTTGTATTAAATTTTTTTTAATTCACAGAAATATAATTTTAAAAAAAGCACTTTGATTCACAGTCAACAATTCTCAATATTCAAATAGACATATTATATATTAAATACAATCACATGAAGTTTAATAGTTTGACTTTACAAACTTTATCGAATCTGATGTGTTCATCTTAGAACACGATAGGCTTAATATATATATATATTAATATTTGGTTTAAGATTTGGGTAGAATTGAATAAATGTACACTACCTAGATGTACCGAAAAGGATAAGCATATAAGTTGAAATTTTTTAGCTTTTTAACTAAATTTCTATGATTTATCTTGTTCGAGAGGTTGAAAAAATTGATCCAATGAGTTTTAATTTTTTAAAAAAATGCTAATTTTATAACACAAAAATTTGAGTTATTTCTCATTTTCTTAGTATATATCTTGACAACTGGAAAAATAATTCATTACTTTTTTTGTAGTTAATTCAATGTGGAACATTGAAAAATTAATTGATTTTGAAATTTAAAAAATTTTAATAACGATTTTGGAATTCAATAGAGTTGCATGTGGCCAACTTTGAGCATAACGTAACACCCCAACATTTTAGATCATAATATATTACCAAAAATAACTTTTTCTCCCTTTTCCAATCAAATAATATATATATATATATATGAAATTGATTTTGCTAAAACTCAATAAAAAATTAAAGAAAAAATTGGATTATCAACTAGATAATCCTTAATTCATAGTATGAGTAATTTTATATTTTTAACGCTAATTTTTTTTTTCAATATAGTAAAATTAATTAATTTTTCTTATATATTATAAATATAAAAAAATTAGAATGGTTAAATGATCATCACTTAAAATTCTTTTATAAAAACACCAATTTTTGCAAATGTGAATATCAAACCCATGCAAATGTACTGCCACGTCATGACGGCAAGTTGAAAAATGATGCATCTGATCAAATAGAAGGAGAGAAAATAGTGAGCAATTTCGAGTCTCGCTCTCCTCTACGCTATAAATACAAAACCTTTCCCTGTCTAATTAAGCATCCAAACAAAAGAAAGTCCACATTCAAAAGAAAAATGAAGAATTTCGAAATATTATTCTATTTTCTTTTCATTCTCCTTGCCTCAACTCAGCTGATCCGCTTCTCCACAGCCGACGCTTCGCCGGAGGCCGTCCTCGACATCGACGGCAAGAAGCTCCGAGCCGGCGTCAACTACTACATCCTCCCTGTTTTCCGCGGCAGAGGCGGCGGCCTAACCTTAGGCAACCTCCAATCAGAGAAATGTCCACTCAATGTCGTTCAAGAACAACTAGAAGTAATGAACGGATTTCCAACAACATTTCATCCTGTAAACCCTAAAAAAGGAGTGGTCCGAGTTTCAACCGATTTGAATGTACAATTCGAGGCGAGTACGATCTGCGTGACATCGACGGTGTGGAAATTGGACAAATTCGACGAATCAACAGGACAATGGTTGGTGACGATCGGCGGAAGCAGGGGAAATCCGGGAGTGGAGACGGTGGATAACTGGTTCAAAATTGAGAAGCATGGCAAGGATTACAAATTGGTGTTCTGTCCGACTGTTTGCAATTTCTGTAAAGTTATGTGTAGAGATATTGGAATCTTCTTCAAGAATGGAGAAAGGGCTTTGGCTTTGAGCGAGACGCCATTCCCTGTTATGTTCAAGAAAGTTTAA

mRNA sequence

ATGAAAAACTTTGCATTACTGTGTTTTCTTTTCATTGTCATTGCCTCATCTGAGGTACGCTTCTGCAGAGCGGACGCCTCCCCCGATGCGGTCCTCGACACCGACGGAAAGAAGCTTCGAGCCGGTGATCAATATTACATTCTCTCAGTTTACAACAGAAATAGCGGCGGATTAAGCATCGGCGGTATCTACGGATACGAAAAATGTCCCATCAACATCCTCCCCGAATCTTACGATTATTTACACGGTCTTCCGGCGACGTTTTCCCCCGTAAACCCTAAGAAAGGTGTGGTCCGAGTTTCTACCGATTTGAACATCCAATTCGAGGCGAACACGAGGTGTGGAATATCGACGGTGTGGAAAGTAGGTAAGTTTGATGAATACTTGAAGCAGTATTTTGTGATGATGGGCGGAACGAAAGGGAATCCGGGGTGGGAAACGATCGAAAATTGGTTCAAAGTGGAGAAGTATGGTAAGAATTATAAGTTGGTGTATTGTCCAACAGTTTGTAAGTATTGCAAAGTAATGTGCAAAGATGTGGGATTATTTTACAAGAATGGAAGGAGGGTTATTGCTTTGAATGATGCTCCATTCCCTGTTATGTTCAAGAAAGACTACACTATGGTTATGAAGAAATCTGCATTACTCACTTTTCTTTTCATTGCCATTGTCATTGCCTCATCTGAGTTACGCTTCTGCAGAGCTGATGCGTCACCGGATGCCGTCCTCGACACCGATGGAAAGAAGCTCAGAGTAAGCAACAACGGTGGTGGTGGAGGTTTAGCCATCGGCAATATCCGAAAAGAATACGATAGATGTGGTGTCAACGTCGTCCAGGAGCGTTACGAACAATTCGATGGTTTTCCCACGACATTTTTACCAAAGAACCTTAAAAAGGGTGTGGTTCGAGTCTCCACCGATTTGAACATCGAGTTTGATGGGACTACGAGGTGTGTGAAATCAACGGTGTGGAGATTAGGAACATTTGATCGATATTTGAGGCAATATTTCGTGACGATTGGCGGAACGAAAGGGAACCCGGGGCGAGAGACGACGGAAAATTGGTTCAAAATAGAGAAATATGGGAATGGTTACAAGTTGGTGTATTGTCCAAGAGTGGTGTGTAAGTATTGCAAAGTAATGTGCAAAGATATTGGTATATTCGAGAACAATGGGATAAGGGGTCTTGTTTTGAGCGATACGCCATTTCCCGTTATGTTCAAGAAACTGATCCGCTTCTCCACAGCCGACGCTTCGCCGGAGGCCGTCCTCGACATCGACGGCAAGAAGCTCCGAGCCGGCGTCAACTACTACATCCTCCCTGTTTTCCGCGGCAGAGGCGGCGGCCTAACCTTAGGCAACCTCCAATCAGAGAAATGTCCACTCAATGTCGTTCAAGAACAACTAGAAGTAATGAACGGATTTCCAACAACATTTCATCCTGTAAACCCTAAAAAAGGAGTGGTCCGAGTTTCAACCGATTTGAATGTACAATTCGAGGCGAGTACGATCTGCGTGACATCGACGGTGTGGAAATTGGACAAATTCGACGAATCAACAGGACAATGGTTGGTGACGATCGGCGGAAGCAGGGGAAATCCGGGAGTGGAGACGGTGGATAACTGGTTCAAAATTGAGAAGCATGGCAAGGATTACAAATTGGTGTTCTGTCCGACTGTTTGCAATTTCTGTAAAGTTATGTGTAGAGATATTGGAATCTTCTTCAAGAATGGAGAAAGGGCTTTGGCTTTGAGCGAGACGCCATTCCCTGTTATGTTCAAGAAAGTTTAA

Coding sequence (CDS)

ATGAAAAACTTTGCATTACTGTGTTTTCTTTTCATTGTCATTGCCTCATCTGAGGTACGCTTCTGCAGAGCGGACGCCTCCCCCGATGCGGTCCTCGACACCGACGGAAAGAAGCTTCGAGCCGGTGATCAATATTACATTCTCTCAGTTTACAACAGAAATAGCGGCGGATTAAGCATCGGCGGTATCTACGGATACGAAAAATGTCCCATCAACATCCTCCCCGAATCTTACGATTATTTACACGGTCTTCCGGCGACGTTTTCCCCCGTAAACCCTAAGAAAGGTGTGGTCCGAGTTTCTACCGATTTGAACATCCAATTCGAGGCGAACACGAGGTGTGGAATATCGACGGTGTGGAAAGTAGGTAAGTTTGATGAATACTTGAAGCAGTATTTTGTGATGATGGGCGGAACGAAAGGGAATCCGGGGTGGGAAACGATCGAAAATTGGTTCAAAGTGGAGAAGTATGGTAAGAATTATAAGTTGGTGTATTGTCCAACAGTTTGTAAGTATTGCAAAGTAATGTGCAAAGATGTGGGATTATTTTACAAGAATGGAAGGAGGGTTATTGCTTTGAATGATGCTCCATTCCCTGTTATGTTCAAGAAAGACTACACTATGGTTATGAAGAAATCTGCATTACTCACTTTTCTTTTCATTGCCATTGTCATTGCCTCATCTGAGTTACGCTTCTGCAGAGCTGATGCGTCACCGGATGCCGTCCTCGACACCGATGGAAAGAAGCTCAGAGTAAGCAACAACGGTGGTGGTGGAGGTTTAGCCATCGGCAATATCCGAAAAGAATACGATAGATGTGGTGTCAACGTCGTCCAGGAGCGTTACGAACAATTCGATGGTTTTCCCACGACATTTTTACCAAAGAACCTTAAAAAGGGTGTGGTTCGAGTCTCCACCGATTTGAACATCGAGTTTGATGGGACTACGAGGTGTGTGAAATCAACGGTGTGGAGATTAGGAACATTTGATCGATATTTGAGGCAATATTTCGTGACGATTGGCGGAACGAAAGGGAACCCGGGGCGAGAGACGACGGAAAATTGGTTCAAAATAGAGAAATATGGGAATGGTTACAAGTTGGTGTATTGTCCAAGAGTGGTGTGTAAGTATTGCAAAGTAATGTGCAAAGATATTGGTATATTCGAGAACAATGGGATAAGGGGTCTTGTTTTGAGCGATACGCCATTTCCCGTTATGTTCAAGAAACTGATCCGCTTCTCCACAGCCGACGCTTCGCCGGAGGCCGTCCTCGACATCGACGGCAAGAAGCTCCGAGCCGGCGTCAACTACTACATCCTCCCTGTTTTCCGCGGCAGAGGCGGCGGCCTAACCTTAGGCAACCTCCAATCAGAGAAATGTCCACTCAATGTCGTTCAAGAACAACTAGAAGTAATGAACGGATTTCCAACAACATTTCATCCTGTAAACCCTAAAAAAGGAGTGGTCCGAGTTTCAACCGATTTGAATGTACAATTCGAGGCGAGTACGATCTGCGTGACATCGACGGTGTGGAAATTGGACAAATTCGACGAATCAACAGGACAATGGTTGGTGACGATCGGCGGAAGCAGGGGAAATCCGGGAGTGGAGACGGTGGATAACTGGTTCAAAATTGAGAAGCATGGCAAGGATTACAAATTGGTGTTCTGTCCGACTGTTTGCAATTTCTGTAAAGTTATGTGTAGAGATATTGGAATCTTCTTCAAGAATGGAGAAAGGGCTTTGGCTTTGAGCGAGACGCCATTCCCTGTTATGTTCAAGAAAGTTTAA

Protein sequence

MKNFALLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYNRNSGGLSIGGIYGYEKCPINILPESYDYLHGLPATFSPVNPKKGVVRVSTDLNIQFEANTRCGISTVWKVGKFDEYLKQYFVMMGGTKGNPGWETIENWFKVEKYGKNYKLVYCPTVCKYCKVMCKDVGLFYKNGRRVIALNDAPFPVMFKKDYTMVMKKSALLTFLFIAIVIASSELRFCRADASPDAVLDTDGKKLRVSNNGGGGGLAIGNIRKEYDRCGVNVVQERYEQFDGFPTTFLPKNLKKGVVRVSTDLNIEFDGTTRCVKSTVWRLGTFDRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGNGYKLVYCPRVVCKYCKVMCKDIGIFENNGIRGLVLSDTPFPVMFKKLIRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLGNLQSEKCPLNVVQEQLEVMNGFPTTFHPVNPKKGVVRVSTDLNVQFEASTICVTSTVWKLDKFDESTGQWLVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIGIFFKNGERALALSETPFPVMFKKV*

Homology

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BLAST of Chy12G219550 vs. ExPASy Swiss-Prot
Match: Q9LMU2 (Kunitz trypsin inhibitor 5 OS=Arabidopsis thaliana OX=3702 GN=KTI5 PE=2 SV=1)

HSP 1 Score: 223.8 bits (569), Expect = 5.2e-57
Identity = 101/181 (55.80%), Postives = 132/181 (72.93%), Query Frame = 0


Query: 415 TADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLGNLQSEKCPLNVVQEQLEVMNG 474
           T +A+ E V DI+GK L  GVNYYILPV RGRGGGLT+ NL++E CP +V+Q+Q EV  G
Sbjct: 21  TTEAAVEPVKDINGKSLLTGVNYYILPVIRGRGGGLTMSNLKTETCPTSVIQDQFEVSQG 80

Query: 475 FPTTFHPVNPKKGVVRVSTDLNVQFEASTICVTSTVWKLDKFDESTGQWLVTIGGSRGNP 534
            P  F P + K   + VSTD+N++F  ++I      W+L  FDE+T QW ++  G  GNP
Sbjct: 81  LPVKFSPYD-KSRTIPVSTDVNIKFSPTSI------WELANFDETTKQWFISTCGVEGNP 140

Query: 535 GVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIGIFFKNGERALALSETPFPVMFK 594
           G +TVDNWFKI+K  KDYK+ FCPTVCNFCKV+CRD+G+F ++G+R LALS+ P  VMFK
Sbjct: 141 GQKTVDNWFKIDKFEKDYKIRFCPTVCNFCKVICRDVGVFVQDGKRRLALSDVPLKVMFK 194

Query: 595 K 596
           +
Sbjct: 201 R 194

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BLAST of Chy12G219550 vs. ExPASy Swiss-Prot
Match: P13087 (Miraculin OS=Synsepalum dulcificum OX=3743 PE=1 SV=3)

HSP 1 Score: 210.7 bits (535), Expect = 4.6e-53
Identity = 107/190 (56.32%), Postives = 128/190 (67.37%), Query Frame = 0


Query: 414 STADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLGNLQSE---KCPLNVVQEQLE 473
           S AD++P  VLDIDG+KLR G NYYI+PV R  GGGLT+          CP  VVQ + E
Sbjct: 27  SAADSAPNPVLDIDGEKLRTGTNYYIVPVLRDHGGGLTVSATTPNGTFVCPPRVVQTRKE 86

Query: 474 VMNGFPTTFHPVNPKKGVVRVSTDLNVQFEASTIC--VTSTVWKLDKFDESTGQWLVTIG 533
           V +  P  F P NPK+ VVRVSTDLN+ F A   C   +STVW+LDK+DESTGQ+ VTIG
Sbjct: 87  VDHDRPLAFFPENPKEDVVRVSTDLNINFSAFMPCRWTSSTVWRLDKYDESTGQYFVTIG 146

Query: 534 GSRGNPGVETVDNWFKIEKH--GKDYKLVFCPTVCNFCKVMCRDIGIFF-KNGERALALS 593
           G +GNPG ET+ +WFKIE+      YKLVFCPTVC  CKV C D+GI+  + G R LALS
Sbjct: 147 GVKGNPGPETISSWFKIEEFCGSGFYKLVFCPTVCGSCKVKCGDVGIYIDQKGRRRLALS 206

Query: 594 ETPFPVMFKK 596
           + PF   F K
Sbjct: 207 DKPFAFEFNK 216

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BLAST of Chy12G219550 vs. ExPASy Swiss-Prot
Match: P32765 (21 kDa seed protein OS=Theobroma cacao OX=3641 GN=ASP PE=2 SV=1)

HSP 1 Score: 168.3 bits (425), Expect = 2.6e-40
Identity = 87/188 (46.28%), Postives = 114/188 (60.64%), Query Frame = 0


Query: 413 FSTADASPEAVLDIDGKKLRAGVNYYILPVFRGR-GGGLTLGNLQSEKCPLNVVQEQLEV 472
           F  A+A+   VLD DG +L+ GV YY+L    G  GGGL LG    + CP  VVQ + ++
Sbjct: 21  FGVANAANSPVLDTDGDELQTGVQYYVLSSISGAGGGGLALGRATGQSCPEIVVQRRSDL 80

Query: 473 MNGFPTTFHPVNPKKGVVRVSTDLNVQFE--ASTICVTSTVWKLDKFDESTGQWLVTIGG 532
            NG P  F   + K  VVRVSTD+N++F      +C TSTVW+LD +D S G+W VT  G
Sbjct: 81  DNGTPVIFSNADSKDDVVRVSTDVNIEFVPIRDRLCSTSTVWRLDNYDNSAGKWWVTTDG 140

Query: 533 SRGNPGVETVDNWFKIEKHG-KDYKLVFCPTVCNFCKVMCRDIGIFF-KNGERALALSET 592
            +G PG  T+ +WFKIEK G   YK  FCP+VC+ C  +C DIG     +G+  LALS+ 
Sbjct: 141 VKGEPGPNTLCSWFKIEKAGVLGYKFRFCPSVCDSCTTLCSDIGRHSDDDGQIRLALSDN 200

Query: 593 PFPVMFKK 596
            +  MFKK
Sbjct: 201 EWAWMFKK 208

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BLAST of Chy12G219550 vs. ExPASy Swiss-Prot
Match: Q8RXD5 (Kunitz trypsin inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=KTI4 PE=2 SV=1)

HSP 1 Score: 147.9 bits (372), Expect = 3.6e-34
Identity = 79/177 (44.63%), Postives = 105/177 (59.32%), Query Frame = 0


Query: 422 AVLDIDGKKLRAGVNYYILPVFRGRGGGLTLGNLQSEKCPLNVVQEQLEVMNGFPTTFHP 481
           AV+DIDG  +    +YY+LPV RGRGGGLTL     + CP ++VQE  EV  G P  F  
Sbjct: 29  AVVDIDGNAM-FHESYYVLPVIRGRGGGLTLAGRGGQPCPYDIVQESSEVDEGIPVKFSN 88

Query: 482 VNPKKGVVRVSTDLNVQFE-ASTICVTSTVWKLDKFDESTGQWLVTIGGSRGNPGVETVD 541
              K   V  S +LN++ +  +TIC+ ST W++ +FD    Q+ V  G      G +++ 
Sbjct: 89  WRLKVAFVPESQNLNIETDVGATICIQSTYWRVGEFDHERKQYFVVAGPKPEGFGQDSLK 148

Query: 542 NWFKIEKHGKD-YKLVFCPTVCNFCKVMCRDIGIFFKN-GERALALSETPFPVMFKK 596
           ++FKIEK G+D YK VFCP  C+     C D+GIF    G R LALS+ PF VMFKK
Sbjct: 149 SFFKIEKSGEDAYKFVFCPRTCDSGNPKCSDVGIFIDELGVRRLALSDKPFLVMFKK 204

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BLAST of Chy12G219550 vs. ExPASy Swiss-Prot
Match: P80691 (Latex serine proteinase inhibitor OS=Carica papaya OX=3649 PE=1 SV=1)

HSP 1 Score: 115.2 bits (287), Expect = 2.6e-24
Identity = 65/162 (40.12%), Postives = 95/162 (58.64%), Query Frame = 0


Query: 419 SPEAVLDIDGKKLRAGVNYYILPVFRGR-GGGLTL-GNLQSEKCPLNVVQEQLEVMNGFP 478
           +P+ ++DIDGK +  GV+Y+++    G  GGGLT+ G    +KCPL+VVQ+  +  NG P
Sbjct: 2   APKPIVDIDGKPVLYGVDYFVVSAIWGAGGGGLTVYGPGNKKKCPLSVVQDPFD--NGEP 61

Query: 479 TTFHPV-NPKKGVVRVSTDLNVQFEASTICVTSTVWKLDKFDESTGQWLVTIGGSRGNPG 538
             F  + N K  +V  S DLNV+F  +  C  +T WK+D+F    G W VT+GG +G  G
Sbjct: 62  IIFSAIKNVKDNIVFESVDLNVKFNITINCNETTAWKVDRFPGVIG-WTVTLGGEKGYHG 121

Query: 539 VETVDNWFKIEKHGK--DYKLVFCPTVCNFCKVMCRDIGIFF 576
            E+  + FKI+K G    YK  FCP+      + C ++ IFF
Sbjct: 122 FESTHSMFKIKKAGLPFSYKFHFCPSYPRTRLIPCNNVDIFF 160

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BLAST of Chy12G219550 vs. ExPASy TrEMBL
Match: A0A103YID5 (Kunitz inhibitor ST1-like protein OS=Cynara cardunculus var. scolymus OX=59895 GN=Ccrd_011933 PE=3 SV=1)

HSP 1 Score: 488.4 bits (1256), Expect = 4.2e-134
Identity = 268/597 (44.89%), Postives = 343/597 (57.45%), Query Frame = 0


Query: 10  LFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYNRNSGGLSIGGIYGYEKC 69
           L +++  S +  C    SPD VLD DG  +R+G  YYIL V+    GG+++      E C
Sbjct: 4   LSLLLLFSTISLCLGQPSPDPVLDIDGNLVRSGTDYYILPVFRGMGGGVTLASTRN-ESC 63

Query: 70  PINILPESYDYLHGLPATFSPVNPKKGVVRVSTDLNIQFEANTRCGISTVWKVGKFDEYL 129
           P++++ E  +  +GLP TF+PV+PKKGV+R STDLNI F A + C  S VW +   +EY 
Sbjct: 64  PLDVVQEPQEVDNGLPLTFTPVDPKKGVIRESTDLNIIFSALSICIQSNVWNL---EEYE 123

Query: 130 KQYFVMMGGTKGNPGWETIENWFKVEKYGKNYKLVYCPTVCKYCKVMCKDVGLFY-KNGR 189
            Q  V   G  GNPG ETI NWFK+EKY  +YK+V+CPTVC +C+ +C D+G+   ++G 
Sbjct: 124 GQLIVSARGVSGNPGQETISNWFKIEKYEDDYKIVFCPTVCDFCRPVCGDIGVIIGEDGS 183

Query: 190 RVIALNDAPFPVMFKKDYTMVMKKSALLTFLFIAIVIASSELRFCRADASPDAVLDTDGK 249
           R +A+ D PF +           K+ LL  LF  I ++  +        SPD V DTDG 
Sbjct: 184 RRLAIRDVPFKM-----------KTVLLFLLFSTISLSFGQ-------KSPDQVRDTDGN 243

Query: 250 KLRVSNN--------GGGGGLAIGNIRKEYDRCGVNVVQERYEQFDGFPTTFLPKNLKKG 309
            LR   +        G GGG+ +   R E   C ++VVQE +E   G P  F P N KKG
Sbjct: 244 LLRSGTDYYILPVFRGRGGGVTLAPTRNE--SCPLDVVQEGFELKFGLPLKFTPVNSKKG 303

Query: 310 VVRVSTDLNIEFDGTTRCVKSTVWRLGTFDRYLRQYFVTIGGTKGNPGRETTENWFKIEK 369
           V+R STDLNI F  +T CV+S VW L   + Y  Q  V+  G  GNPGRET  NWFKI K
Sbjct: 304 VIRESTDLNIMFSASTSCVQSNVWML---EAYKGQLIVSGHGVWGNPGRETISNWFKIAK 363

Query: 370 YGNGYKLVYCPRVVCKYCKVMCKDIGI-FENNGIRGLVLSDTPFPVMFKKLIRFSTADAS 429
           Y N YKLV+CP  VC YCK +C DIG+    NG R                         
Sbjct: 364 YENDYKLVFCP-TVCNYCKPVCGDIGVKIAENGSR------------------------- 423

Query: 430 PEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLGNLQSEKCPLNVVQEQLEVMNGFPTTF 489
                           +YYILPVFRGRGGG+TL   ++E CPL+VVQE  E+  G P  F
Sbjct: 424 ----------------HYYILPVFRGRGGGVTLAPTRNESCPLDVVQEGFELKFGLPLQF 483

Query: 490 HPVNPKKGVVRVSTDLNVQFEASTICVTSTVWKLDKFDESTGQWLVTIGGSRGNPGVETV 549
            PVN KKGV+R STDLN+ F AST C  S  W L+ +    GQ +V+  G  GNPG ET+
Sbjct: 484 APVNFKKGVIRESTDLNIXFSASTXCXQSNXWMLEAY---XGQLIVSGHGVWGNPGRETI 528

Query: 550 DNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIGI-FFKNGERALALSETPFPVMFKK 596
            NWFKI K+  DYKLVFCPTVCN+CK +C DIG+   +NG R LA+S+ PF V F+K
Sbjct: 544 SNWFKIAKYENDYKLVFCPTVCNYCKPVCGDIGVKIAENGSRRLAISDVPFKVKFRK 528

[Close]

BLAST of Chy12G219550 vs. ExPASy TrEMBL
Match: A0A444YYM6 (Uncharacterized protein OS=Arachis hypogaea OX=3818 GN=Ahy_B05g074359 PE=3 SV=1)

HSP 1 Score: 427.9 bits (1099), Expect = 6.7e-116
Identity = 263/610 (43.11%), Postives = 344/610 (56.39%), Query Frame = 0


Query: 24  ADASPDAVLDTDGKKLRAGDQYYILSVYNR---NSGGLSIGGIY------GYEKCPINIL 83
           AD SP+ V+DT+GKK+RAG +YYI  V        G   +G  +        E CP+N++
Sbjct: 204 ADPSPEQVVDTEGKKVRAGIEYYIRPVPTTPCDGRGPCVVGSGFVLVARSANETCPLNVV 263

Query: 84  PESYDYLHGLPATFSPVNPKKGVVRVSTDLNIQFEANTRCGISTVWKVGKFDEYLKQYFV 143
               +   G    F+PVNPKKGV+RVSTD+NI+    T C  STVWK+  FD    Q+FV
Sbjct: 264 --VVEGFRGQAVIFTPVNPKKGVIRVSTDVNIKTNLTTICTESTVWKLDDFDSSSGQWFV 323

Query: 144 MMGGTKGNPGWETIENWFKVEKYGKNYKLVYCPTVCKYCKVMCKDVGLFY-KNGRRVIAL 203
             GG  GNPG +TI NWFK+EKY  +YKLV+CPTVC +CK +CK+VG+F   NG R +AL
Sbjct: 324 TTGGVVGNPGKDTISNWFKIEKYDDDYKLVFCPTVCDFCKPLCKNVGVFVDSNGNRRVAL 383

Query: 204 NDAPFPVMFKKDYTMVMKKSALLTFLFIAIVIASSELRFCRADA-SPDAVLDTDGKKLRV 263
            D P+             K  L   L + + IA S      A A +P+ VLDT GKK+R 
Sbjct: 384 TDQPY-------------KITLFLSLLVVLAIALSTKPLPGAAAYAPEPVLDTSGKKVRT 443

Query: 264 --------SNNGGGGGLAIGNIRKEYDRCGVNVVQERYEQFDG-FPTTFLPKNLKKGVVR 323
                   ++    GG++IG++ +    C   VV    +  +G  P    P N KKGV+R
Sbjct: 444 NVAYNVIPASYPYVGGVSIGSVNQ---TCPFYVVGG--DSSNGPLPLYLSPVNEKKGVIR 503

Query: 324 VSTDLNIEF--DGTTR-CVKSTVWRLGTFDRYLRQYFVTIGGTKGNPGRETTENWFKIEK 383
           V TDLNI F   GT   C  S+VW L  +D    Q FVT+GG  GNPG+ET  +WFKIEK
Sbjct: 504 VHTDLNIYFLDPGTDEVCSDSSVWMLKDYDSSATQKFVTLGGAMGNPGKETLADWFKIEK 563

Query: 384 YGNGYKLVYCPRVVCKYCKVMCKDIGIFENN-GIRGLVLSDTPFPV-MFKKL-----IRF 443
           Y + YK  YCP V        C DIGI E+  G + L LSD P+ + +F  L     I  
Sbjct: 564 YEDAYKFYYCPNVYYD-GSYPCSDIGISEDEYGNKRLTLSDVPYKITLFLALFVVLAIAL 623

Query: 444 ST------ADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLGNLQSEKCPLNVVQE 503
           ST      A ++PE VLD  GKK+R  VNYYI+P      GG+T+ ++ ++ CP NV+  
Sbjct: 624 STKPLLGAAASAPETVLDTSGKKVRTSVNYYIVPGSYPYAGGITVASV-NQTCPFNVIAG 683

Query: 504 QLEVMNG-FPTTFHPVNPKKGVVRVSTDLNVQFEASTI-CVTSTVWKLDKFDESTGQWLV 563
             +  NG  P    PVN KKGV+R+ TDLN+ F    + C  STVW L  +D S  Q  V
Sbjct: 684 --DSSNGPLPLYLLPVNEKKGVIRIHTDLNIFFSDGGVGCPDSTVWMLKDYDSSAAQKFV 743

Query: 564 TIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIGIF-FKNGERALAL 595
           T+GG+ GNPG ET+ +WFKIEK+   YKL +CP V       C DIGI   + G + LAL
Sbjct: 744 TLGGALGNPGKETLADWFKIEKYEDAYKLYYCPNVYYDGSYPCSDIGISEDEYGNKRLAL 789

[Close]

BLAST of Chy12G219550 vs. ExPASy TrEMBL
Match: A0A0A0LT81 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G043220 PE=3 SV=1)

HSP 1 Score: 419.9 bits (1078), Expect = 1.8e-113
Identity = 197/204 (96.57%), Postives = 201/204 (98.53%), Query Frame = 0


Query: 1   MKNFALLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYNRNSGGLSI 60
           M+NFALLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVY+RNSGGLSI
Sbjct: 1   MRNFALLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGLSI 60

Query: 61  GGIYGYEKCPINILPESYDYLHGLPATFSPVNPKKGVVRVSTDLNIQFEANTRCGISTVW 120
           GGIYGYEKCPINILPESYDYLHGLPATFSP+NPKKGVVRVSTDLNIQFEANTRCGISTVW
Sbjct: 61  GGIYGYEKCPINILPESYDYLHGLPATFSPINPKKGVVRVSTDLNIQFEANTRCGISTVW 120

Query: 121 KVGKFDEYLKQYFVMMGGTKGNPGWETIENWFKVEKYGKNYKLVYCPTVCKYCKVMCKDV 180
           KVGKFDEYLKQYFV MGG KGNPG ETIENWFKVEKYGKNYKLVYCPTVCKYCKV+CKDV
Sbjct: 121 KVGKFDEYLKQYFVTMGGMKGNPGRETIENWFKVEKYGKNYKLVYCPTVCKYCKVVCKDV 180

Query: 181 GLFYKNGRRVIALNDAPFPVMFKK 205
           GLFYKNGRRVIALNDAPFPVMFKK
Sbjct: 181 GLFYKNGRRVIALNDAPFPVMFKK 204

[Close]

BLAST of Chy12G219550 vs. ExPASy TrEMBL
Match: A0A5D3BJ02 (Miraculin OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G002800 PE=3 SV=1)

HSP 1 Score: 409.5 bits (1051), Expect = 2.5e-110
Identity = 192/204 (94.12%), Postives = 198/204 (97.06%), Query Frame = 0


Query: 1   MKNFALLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYNRNSGGLSI 60
           MKNFALLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVY+RNSGGLSI
Sbjct: 1   MKNFALLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGLSI 60

Query: 61  GGIYGYEKCPINILPESYDYLHGLPATFSPVNPKKGVVRVSTDLNIQFEANTRCGISTVW 120
           GGIYGYEKCPINILPESYDYL GLPATFSPVNPKKGVVRVSTDLNI+FEA+TRCGISTVW
Sbjct: 61  GGIYGYEKCPINILPESYDYLDGLPATFSPVNPKKGVVRVSTDLNIEFEASTRCGISTVW 120

Query: 121 KVGKFDEYLKQYFVMMGGTKGNPGWETIENWFKVEKYGKNYKLVYCPTVCKYCKVMCKDV 180
           KVGKFD+YLKQYFV MGGTKGNPG ETI NWFKVEK+GKNYK VYCPTVCKYCKVMCKDV
Sbjct: 121 KVGKFDQYLKQYFVTMGGTKGNPGRETIGNWFKVEKHGKNYKFVYCPTVCKYCKVMCKDV 180

Query: 181 GLFYKNGRRVIALNDAPFPVMFKK 205
           GLFYKNGRR+ ALNDAPFPVMFKK
Sbjct: 181 GLFYKNGRRIFALNDAPFPVMFKK 204

[Close]

BLAST of Chy12G219550 vs. ExPASy TrEMBL
Match: A0A1S3AST3 (miraculin OS=Cucumis melo OX=3656 GN=LOC103482610 PE=3 SV=1)

HSP 1 Score: 409.5 bits (1051), Expect = 2.5e-110
Identity = 192/204 (94.12%), Postives = 198/204 (97.06%), Query Frame = 0


Query: 1   MKNFALLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYNRNSGGLSI 60
           MKNFALLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVY+RNSGGLSI
Sbjct: 1   MKNFALLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGLSI 60

Query: 61  GGIYGYEKCPINILPESYDYLHGLPATFSPVNPKKGVVRVSTDLNIQFEANTRCGISTVW 120
           GGIYGYEKCPINILPESYDYL GLPATFSPVNPKKGVVRVSTDLNI+FEA+TRCGISTVW
Sbjct: 61  GGIYGYEKCPINILPESYDYLDGLPATFSPVNPKKGVVRVSTDLNIEFEASTRCGISTVW 120

Query: 121 KVGKFDEYLKQYFVMMGGTKGNPGWETIENWFKVEKYGKNYKLVYCPTVCKYCKVMCKDV 180
           KVGKFD+YLKQYFV MGGTKGNPG ETI NWFKVEK+GKNYK VYCPTVCKYCKVMCKDV
Sbjct: 121 KVGKFDQYLKQYFVTMGGTKGNPGRETIGNWFKVEKHGKNYKFVYCPTVCKYCKVMCKDV 180

Query: 181 GLFYKNGRRVIALNDAPFPVMFKK 205
           GLFYKNGRR+ ALNDAPFPVMFKK
Sbjct: 181 GLFYKNGRRIFALNDAPFPVMFKK 204

[Close]

BLAST of Chy12G219550 vs. NCBI nr
Match: KVI09680.1 (Kunitz inhibitor ST1-like protein [Cynara cardunculus var. scolymus])

HSP 1 Score: 481 bits (1239), Expect = 3.02e-161
Identity = 268/597 (44.89%), Postives = 343/597 (57.45%), Query Frame = 0


Query: 10  LFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYNRNSGGLSIGGIYGYEKC 69
           L +++  S +  C    SPD VLD DG  +R+G  YYIL V+    GG+++      E C
Sbjct: 4   LSLLLLFSTISLCLGQPSPDPVLDIDGNLVRSGTDYYILPVFRGMGGGVTLASTRN-ESC 63

Query: 70  PINILPESYDYLHGLPATFSPVNPKKGVVRVSTDLNIQFEANTRCGISTVWKVGKFDEYL 129
           P++++ E  +  +GLP TF+PV+PKKGV+R STDLNI F A + C  S VW +   +EY 
Sbjct: 64  PLDVVQEPQEVDNGLPLTFTPVDPKKGVIRESTDLNIIFSALSICIQSNVWNL---EEYE 123

Query: 130 KQYFVMMGGTKGNPGWETIENWFKVEKYGKNYKLVYCPTVCKYCKVMCKDVGLFY-KNGR 189
            Q  V   G  GNPG ETI NWFK+EKY  +YK+V+CPTVC +C+ +C D+G+   ++G 
Sbjct: 124 GQLIVSARGVSGNPGQETISNWFKIEKYEDDYKIVFCPTVCDFCRPVCGDIGVIIGEDGS 183

Query: 190 RVIALNDAPFPVMFKKDYTMVMKKSALLTFLFIAIVIASSELRFCRADASPDAVLDTDGK 249
           R +A+ D PF +           K+ LL  LF  I ++  +        SPD V DTDG 
Sbjct: 184 RRLAIRDVPFKM-----------KTVLLFLLFSTISLSFGQ-------KSPDQVRDTDGN 243

Query: 250 KLRVSNN--------GGGGGLAIGNIRKEYDRCGVNVVQERYEQFDGFPTTFLPKNLKKG 309
            LR   +        G GGG+ +   R E   C ++VVQE +E   G P  F P N KKG
Sbjct: 244 LLRSGTDYYILPVFRGRGGGVTLAPTRNE--SCPLDVVQEGFELKFGLPLKFTPVNSKKG 303

Query: 310 VVRVSTDLNIEFDGTTRCVKSTVWRLGTFDRYLRQYFVTIGGTKGNPGRETTENWFKIEK 369
           V+R STDLNI F  +T CV+S VW L   + Y  Q  V+  G  GNPGRET  NWFKI K
Sbjct: 304 VIRESTDLNIMFSASTSCVQSNVWML---EAYKGQLIVSGHGVWGNPGRETISNWFKIAK 363

Query: 370 YGNGYKLVYCPRVVCKYCKVMCKDIGI-FENNGIRGLVLSDTPFPVMFKKLIRFSTADAS 429
           Y N YKLV+CP  VC YCK +C DIG+    NG R                         
Sbjct: 364 YENDYKLVFCP-TVCNYCKPVCGDIGVKIAENGSR------------------------- 423

Query: 430 PEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLGNLQSEKCPLNVVQEQLEVMNGFPTTF 489
                           +YYILPVFRGRGGG+TL   ++E CPL+VVQE  E+  G P  F
Sbjct: 424 ----------------HYYILPVFRGRGGGVTLAPTRNESCPLDVVQEGFELKFGLPLQF 483

Query: 490 HPVNPKKGVVRVSTDLNVQFEASTICVTSTVWKLDKFDESTGQWLVTIGGSRGNPGVETV 549
            PVN KKGV+R STDLN+ F AST C  S  W L+ +    GQ +V+  G  GNPG ET+
Sbjct: 484 APVNFKKGVIRESTDLNIXFSASTXCXQSNXWMLEAYX---GQLIVSGHGVWGNPGRETI 528

Query: 550 DNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIGI-FFKNGERALALSETPFPVMFKK 595
            NWFKI K+  DYKLVFCPTVCN+CK +C DIG+   +NG R LA+S+ PF V F+K
Sbjct: 544 SNWFKIAKYENDYKLVFCPTVCNYCKPVCGDIGVKIAENGSRRLAISDVPFKVKFRK 528

[Close]

BLAST of Chy12G219550 vs. NCBI nr
Match: XP_008437082.1 (PREDICTED: miraculin [Cucumis melo] >TYJ99076.1 miraculin [Cucumis melo var. makuwa])

HSP 1 Score: 406 bits (1043), Expect = 1.61e-136
Identity = 193/207 (93.24%), Postives = 199/207 (96.14%), Query Frame = 0


Query: 1   MKNFALLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYNRNSGGLSI 60
           MKNFALLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVY+RNSGGLSI
Sbjct: 1   MKNFALLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGLSI 60

Query: 61  GGIYGYEKCPINILPESYDYLHGLPATFSPVNPKKGVVRVSTDLNIQFEANTRCGISTVW 120
           GGIYGYEKCPINILPESYDYL GLPATFSPVNPKKGVVRVSTDLNI+FEA+TRCGISTVW
Sbjct: 61  GGIYGYEKCPINILPESYDYLDGLPATFSPVNPKKGVVRVSTDLNIEFEASTRCGISTVW 120

Query: 121 KVGKFDEYLKQYFVMMGGTKGNPGWETIENWFKVEKYGKNYKLVYCPTVCKYCKVMCKDV 180
           KVGKFD+YLKQYFV MGGTKGNPG ETI NWFKVEK+GKNYK VYCPTVCKYCKVMCKDV
Sbjct: 121 KVGKFDQYLKQYFVTMGGTKGNPGRETIGNWFKVEKHGKNYKFVYCPTVCKYCKVMCKDV 180

Query: 181 GLFYKNGRRVIALNDAPFPVMFKKDYT 207
           GLFYKNGRR+ ALNDAPFPVMFKK  T
Sbjct: 181 GLFYKNGRRIFALNDAPFPVMFKKVRT 207

[Close]

BLAST of Chy12G219550 vs. NCBI nr
Match: RYR07039.1 (hypothetical protein Ahy_B05g074359 [Arachis hypogaea])

HSP 1 Score: 420 bits (1079), Expect = 8.47e-134
Identity = 262/618 (42.39%), Postives = 347/618 (56.15%), Query Frame = 0


Query: 15  ASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYNRNSGGLS---IGGIY------G 74
           ++  V    AD SP+ V+DT+GKK+RAG +YYI  V      G     +G  +       
Sbjct: 195 SNGNVLVGEADPSPEQVVDTEGKKVRAGIEYYIRPVPTTPCDGRGPCVVGSGFVLVARSA 254

Query: 75  YEKCPINILPESYDYLHGLPATFSPVNPKKGVVRVSTDLNIQFEANTRCGISTVWKVGKF 134
            E CP+N++    +   G    F+PVNPKKGV+RVSTD+NI+    T C  STVWK+  F
Sbjct: 255 NETCPLNVVVV--EGFRGQAVIFTPVNPKKGVIRVSTDVNIKTNLTTICTESTVWKLDDF 314

Query: 135 DEYLKQYFVMMGGTKGNPGWETIENWFKVEKYGKNYKLVYCPTVCKYCKVMCKDVGLFY- 194
           D    Q+FV  GG  GNPG +TI NWFK+EKY  +YKLV+CPTVC +CK +CK+VG+F  
Sbjct: 315 DSSSGQWFVTTGGVVGNPGKDTISNWFKIEKYDDDYKLVFCPTVCDFCKPLCKNVGVFVD 374

Query: 195 KNGRRVIALNDAPFPVMFKKDYTMVMKKSALLTFLFIAIVIASSELRFCRADASPDAVLD 254
            NG R +AL D P+ +       +V+           AI +++  L    A A P+ VLD
Sbjct: 375 SNGNRRVALTDQPYKITLFLSLLVVL-----------AIALSTKPLPGAAAYA-PEPVLD 434

Query: 255 TDGKKLRVS--------NNGGGGGLAIGNIRKEYDRCGVNVVQERYEQFDG-FPTTFLPK 314
           T GKK+R +        +    GG++IG++ +    C   VV    +  +G  P    P 
Sbjct: 435 TSGKKVRTNVAYNVIPASYPYVGGVSIGSVNQT---CPFYVVGG--DSSNGPLPLYLSPV 494

Query: 315 NLKKGVVRVSTDLNIEF--DGTTR-CVKSTVWRLGTFDRYLRQYFVTIGGTKGNPGRETT 374
           N KKGV+RV TDLNI F   GT   C  S+VW L  +D    Q FVT+GG  GNPG+ET 
Sbjct: 495 NEKKGVIRVHTDLNIYFLDPGTDEVCSDSSVWMLKDYDSSATQKFVTLGGAMGNPGKETL 554

Query: 375 ENWFKIEKYGNGYKLVYCPRVVCKYCKVMCKDIGIFENN-GIRGLVLSDTPFPV-MFKKL 434
            +WFKIEKY + YK  YCP V        C DIGI E+  G + L LSD P+ + +F  L
Sbjct: 555 ADWFKIEKYEDAYKFYYCPNVYYDG-SYPCSDIGISEDEYGNKRLTLSDVPYKITLFLAL 614

Query: 435 -----IRFST------ADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLGNLQSEK 494
                I  ST      A ++PE VLD  GKK+R  VNYYI+P      GG+T+ ++ ++ 
Sbjct: 615 FVVLAIALSTKPLLGAAASAPETVLDTSGKKVRTSVNYYIVPGSYPYAGGITVASV-NQT 674

Query: 495 CPLNVVQEQLEVMNG-FPTTFHPVNPKKGVVRVSTDLNVQFEASTI-CVTSTVWKLDKFD 554
           CP NV+    +  NG  P    PVN KKGV+R+ TDLN+ F    + C  STVW L  +D
Sbjct: 675 CPFNVIAG--DSSNGPLPLYLLPVNEKKGVIRIHTDLNIFFSDGGVGCPDSTVWMLKDYD 734

Query: 555 ESTGQWLVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIGIFFKN 594
            S  Q  VT+GG+ GNPG ET+ +WFKIEK+   YKL +CP V       C DIGI    
Sbjct: 735 SSAAQKFVTLGGALGNPGKETLADWFKIEKYEDAYKLYYCPNVYYDGSYPCSDIGISEDE 789

[Close]

BLAST of Chy12G219550 vs. NCBI nr
Match: XP_004137534.1 (kunitz trypsin inhibitor 5 [Cucumis sativus])

HSP 1 Score: 391 bits (1005), Expect = 8.56e-131
Identity = 186/188 (98.94%), Postives = 188/188 (100.00%), Query Frame = 0


Query: 409 KLIRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLGNLQSEKCPLNVVQEQ 468
           +LIRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLGNLQSEKCPLNVVQEQ
Sbjct: 19  QLIRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLGNLQSEKCPLNVVQEQ 78

Query: 469 LEVMNGFPTTFHPVNPKKGVVRVSTDLNVQFEASTICVTSTVWKLDKFDESTGQWLVTIG 528
           LEVMNGFPTTFHPVNPKKGVVRVSTDLNVQFEASTICVTSTVWKLDKFDESTGQWLVTIG
Sbjct: 79  LEVMNGFPTTFHPVNPKKGVVRVSTDLNVQFEASTICVTSTVWKLDKFDESTGQWLVTIG 138

Query: 529 GSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIGIFFKNGERALALSETP 588
           GSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIGIFFKNGERALALS+TP
Sbjct: 139 GSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIGIFFKNGERALALSDTP 198

Query: 589 FPVMFKKV 596
           FPVMFKKV
Sbjct: 199 FPVMFKKV 206

[Close]

BLAST of Chy12G219550 vs. NCBI nr
Match: KAE8652565.1 (hypothetical protein Csa_014019 [Cucumis sativus])

HSP 1 Score: 392 bits (1008), Expect = 1.35e-130
Identity = 192/211 (91.00%), Postives = 196/211 (92.89%), Query Frame = 0


Query: 386 GIFENNGIRGLVLSDTPFPVMFKKLIRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRG 445
           GI E N I  +V + +        LIRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRG
Sbjct: 39  GISEKNTI-DVVSTSSRSVTNNPMLIRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRG 98

Query: 446 RGGGLTLGNLQSEKCPLNVVQEQLEVMNGFPTTFHPVNPKKGVVRVSTDLNVQFEASTIC 505
           RGGGLTLGNLQSEKCPLNVVQEQLEVMNGFPTTFHPVNPKKGVVRVSTDLNVQFEASTIC
Sbjct: 99  RGGGLTLGNLQSEKCPLNVVQEQLEVMNGFPTTFHPVNPKKGVVRVSTDLNVQFEASTIC 158

Query: 506 VTSTVWKLDKFDESTGQWLVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCK 565
           VTSTVWKLDKFDESTGQWLVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCK
Sbjct: 159 VTSTVWKLDKFDESTGQWLVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCK 218

Query: 566 VMCRDIGIFFKNGERALALSETPFPVMFKKV 596
           VMCRDIGIFFKNGERALALS+TPFPVMFKKV
Sbjct: 219 VMCRDIGIFFKNGERALALSDTPFPVMFKKV 248

[Close]

BLAST of Chy12G219550 vs. TAIR 10
Match: AT1G17860.1 (Kunitz family trypsin and protease inhibitor protein )

HSP 1 Score: 223.8 bits (569), Expect = 3.7e-58
Identity = 101/181 (55.80%), Postives = 132/181 (72.93%), Query Frame = 0


Query: 415 TADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLGNLQSEKCPLNVVQEQLEVMNG 474
           T +A+ E V DI+GK L  GVNYYILPV RGRGGGLT+ NL++E CP +V+Q+Q EV  G
Sbjct: 21  TTEAAVEPVKDINGKSLLTGVNYYILPVIRGRGGGLTMSNLKTETCPTSVIQDQFEVSQG 80

Query: 475 FPTTFHPVNPKKGVVRVSTDLNVQFEASTICVTSTVWKLDKFDESTGQWLVTIGGSRGNP 534
            P  F P + K   + VSTD+N++F  ++I      W+L  FDE+T QW ++  G  GNP
Sbjct: 81  LPVKFSPYD-KSRTIPVSTDVNIKFSPTSI------WELANFDETTKQWFISTCGVEGNP 140

Query: 535 GVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIGIFFKNGERALALSETPFPVMFK 594
           G +TVDNWFKI+K  KDYK+ FCPTVCNFCKV+CRD+G+F ++G+R LALS+ P  VMFK
Sbjct: 141 GQKTVDNWFKIDKFEKDYKIRFCPTVCNFCKVICRDVGVFVQDGKRRLALSDVPLKVMFK 194

Query: 595 K 596
           +
Sbjct: 201 R 194

[Close]

BLAST of Chy12G219550 vs. TAIR 10
Match: AT1G73260.1 (kunitz trypsin inhibitor 1 )

HSP 1 Score: 147.9 bits (372), Expect = 2.6e-35
Identity = 79/177 (44.63%), Postives = 105/177 (59.32%), Query Frame = 0


Query: 422 AVLDIDGKKLRAGVNYYILPVFRGRGGGLTLGNLQSEKCPLNVVQEQLEVMNGFPTTFHP 481
           AV+DIDG  +    +YY+LPV RGRGGGLTL     + CP ++VQE  EV  G P  F  
Sbjct: 29  AVVDIDGNAM-FHESYYVLPVIRGRGGGLTLAGRGGQPCPYDIVQESSEVDEGIPVKFSN 88

Query: 482 VNPKKGVVRVSTDLNVQFE-ASTICVTSTVWKLDKFDESTGQWLVTIGGSRGNPGVETVD 541
              K   V  S +LN++ +  +TIC+ ST W++ +FD    Q+ V  G      G +++ 
Sbjct: 89  WRLKVAFVPESQNLNIETDVGATICIQSTYWRVGEFDHERKQYFVVAGPKPEGFGQDSLK 148

Query: 542 NWFKIEKHGKD-YKLVFCPTVCNFCKVMCRDIGIFFKN-GERALALSETPFPVMFKK 596
           ++FKIEK G+D YK VFCP  C+     C D+GIF    G R LALS+ PF VMFKK
Sbjct: 149 SFFKIEKSGEDAYKFVFCPRTCDSGNPKCSDVGIFIDELGVRRLALSDKPFLVMFKK 204

[Close]

BLAST of Chy12G219550 vs. TAIR 10
Match: AT1G73325.1 (Kunitz family trypsin and protease inhibitor protein )

HSP 1 Score: 93.6 bits (231), Expect = 5.8e-19
Identity = 67/215 (31.16%), Postives = 106/215 (49.30%), Query Frame = 0


Query: 1   MKNFALLCFLFIVIASSEVRFCRADASP-DAVLDTDGKKLRAGDQYYILSVYNRNSGGL- 60
           M+   L      V+++       ADA+P   VLD  G  +++  QYYI+       GGL 
Sbjct: 1   MEKLTLSFITLTVLSAIFTAASAADATPSQVVLDIAGHPVQSNVQYYIIPAKIGTGGGLI 60

Query: 61  -SIGGIYGYEKC-PINILPESYDYLHGLPATFSPVNPKKGVVRVSTDLNIQFEANT-RCG 120
            S   +   + C  ++I+  S  ++ GLP TFSP+N K   V++S  LN++F++    C 
Sbjct: 61  PSNRNLSTQDLCLNLDIVQSSSPFVSGLPVTFSPLNTKVKHVQLSASLNLEFDSTVWLCP 120

Query: 121 ISTVWKVGKFDEYLKQYFVMMGGTKGNPGWETIENWFKVEKYGKNYKLVYCP----TVCK 180
            S VW++      L++ FV +GG KG        +WF++++ G  YKL+YCP      C 
Sbjct: 121 DSKVWRI-DHSVQLRKSFVSIGGQKGKG-----NSWFQIQEDGDAYKLMYCPISSIVACI 180

Query: 181 YCKVMCKDVGLFYKNGRRVIALNDAPFPVMFKKDY 207
              +   D G+     RR++   D  F V F+K Y
Sbjct: 181 NVSLEIDDHGV-----RRLVLSTDQSFVVKFQKAY 204

The following BLAST results are available for this feature:

BLAST of Chy12G219550 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LMU2	5.2e-57	55.80	Kunitz trypsin inhibitor 5 OS=Arabidopsis thaliana OX=3702 GN=KTI5 PE=2 SV=1	[more]
P13087	4.6e-53	56.32	Miraculin OS=Synsepalum dulcificum OX=3743 PE=1 SV=3	[more]
P32765	2.6e-40	46.28	21 kDa seed protein OS=Theobroma cacao OX=3641 GN=ASP PE=2 SV=1	[more]
Q8RXD5	3.6e-34	44.63	Kunitz trypsin inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=KTI4 PE=2 SV=1	[more]
P80691	2.6e-24	40.12	Latex serine proteinase inhibitor OS=Carica papaya OX=3649 PE=1 SV=1	[more]

BLAST of Chy12G219550 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A103YID5	4.2e-134	44.89	Kunitz inhibitor ST1-like protein OS=Cynara cardunculus var. scolymus OX=59895 G...	[more]
A0A444YYM6	6.7e-116	43.11	Uncharacterized protein OS=Arachis hypogaea OX=3818 GN=Ahy_B05g074359 PE=3 SV=1	[more]
A0A0A0LT81	1.8e-113	96.57	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G043220 PE=3 SV=1	[more]
A0A5D3BJ02	2.5e-110	94.12	Miraculin OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G002800 PE=...	[more]
A0A1S3AST3	2.5e-110	94.12	miraculin OS=Cucumis melo OX=3656 GN=LOC103482610 PE=3 SV=1	[more]

BLAST of Chy12G219550 vs. NCBI nr


Match Name	E-value	Identity	Description
KVI09680.1	3.02e-161	44.89	Kunitz inhibitor ST1-like protein [Cynara cardunculus var. scolymus]	[more]
XP_008437082.1	1.61e-136	93.24	PREDICTED: miraculin [Cucumis melo] >TYJ99076.1 miraculin [Cucumis melo var. mak...	[more]
RYR07039.1	8.47e-134	42.39	hypothetical protein Ahy_B05g074359 [Arachis hypogaea]	[more]
XP_004137534.1	8.56e-131	98.94	kunitz trypsin inhibitor 5 [Cucumis sativus]	[more]
KAE8652565.1	1.35e-130	91.00	hypothetical protein Csa_014019 [Cucumis sativus]	[more]

BLAST of Chy12G219550 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G17860.1	3.7e-58	55.80	Kunitz family trypsin and protease inhibitor protein	[more]
AT1G73260.1	2.6e-35	44.63	kunitz trypsin inhibitor 1	[more]
AT1G73325.1	5.8e-19	31.16	Kunitz family trypsin and protease inhibitor protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002160	Proteinase inhibitor I3, Kunitz legume	PRINTS	PR00291	KUNITZINHBTR	coord: 565..594 score: 24.51 coord: 542..561 score: 25.29 coord: 422..451 score: 46.34 coord: 460..480 score: 33.71
IPR002160	Proteinase inhibitor I3, Kunitz legume	SMART	SM00452	kul_2	coord: 422..596 e-value: 4.4E-72 score: 255.4 coord: 241..411 e-value: 8.2E-53 score: 191.4 coord: 30..206 e-value: 1.8E-55 score: 200.2
IPR002160	Proteinase inhibitor I3, Kunitz legume	PFAM	PF00197	Kunitz_legume	coord: 242..409 e-value: 3.8E-43 score: 147.4 coord: 31..204 e-value: 2.6E-50 score: 170.7 coord: 423..595 e-value: 2.3E-58 score: 197.0
IPR002160	Proteinase inhibitor I3, Kunitz legume	PANTHER	PTHR33107	KUNITZ TRYPSIN INHIBITOR 2	coord: 214..409 coord: 415..596 coord: 7..204
IPR002160	Proteinase inhibitor I3, Kunitz legume	PROSITE	PS00283	SOYBEAN_KUNITZ	coord: 423..439
IPR002160	Proteinase inhibitor I3, Kunitz legume	PROSITE	PS00283	SOYBEAN_KUNITZ	coord: 31..47
IPR002160	Proteinase inhibitor I3, Kunitz legume	CDD	cd00178	STI	coord: 422..595 e-value: 3.83178E-51 score: 172.142
IPR002160	Proteinase inhibitor I3, Kunitz legume	CDD	cd00178	STI	coord: 30..204 e-value: 5.73131E-52 score: 174.068
IPR002160	Proteinase inhibitor I3, Kunitz legume	CDD	cd00178	STI	coord: 241..409 e-value: 3.29546E-42 score: 147.874
None	No IPR available	GENE3D	2.80.10.50		coord: 239..411 e-value: 9.5E-57 score: 193.6
None	No IPR available	GENE3D	2.80.10.50		coord: 25..204 e-value: 3.4E-64 score: 217.7 coord: 417..596 e-value: 1.0E-74 score: 252.0
None	No IPR available	PANTHER	PTHR33107:SF5	KUNITZ TRYPSIN INHIBITOR 2	coord: 415..596 coord: 7..204
None	No IPR available	PANTHER	PTHR33107:SF5	KUNITZ TRYPSIN INHIBITOR 2	coord: 214..409
IPR011065	Kunitz inhibitor STI-like superfamily	SUPERFAMILY	50386	STI-like	coord: 238..409
IPR011065	Kunitz inhibitor STI-like superfamily	SUPERFAMILY	50386	STI-like	coord: 27..205
IPR011065	Kunitz inhibitor STI-like superfamily	SUPERFAMILY	50386	STI-like	coord: 419..596

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy12G219550.1	Chy12G219550.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
molecular_function	GO:0004866	endopeptidase inhibitor activity