Chy12G219410 (gene) Cucumber (hystrix) v1

Overview


Name	Chy12G219410
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Location	chrH12: 17714863 .. 17716096 (-)
RNA-Seq Expression	Chy12G219410
Synteny	Chy12G219410

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGAACAAATATGAGTGATGAAGAACAACTCCCAGTTGTGAAGTTTTGTTCAGAGAATTTGAAGAGTGGAACTAAGGAATGGGTTTTGACAATGGAGAAAGTTCGGGAAGCTCTTGAGGAATACGGTTGTTTCGTAGCATTATACGACACCGTTTCAGCAGAAGTGAGTTCCAATGTGTTTGATTCTTTAAAAGAGTTGTTTGATGATGTTCCTTTGGAGAGGAAGCTTCAAAATGTTTCTGAGAAGCCATACCATGGCTACTTTGGTCAAAACCCTCTAATGCCCATCCATGAAAGTATGGGAATCGAACACCCCGTTCTCCCCACCAATATCAACTCCTTCACTAATCTCATGTGGCCTTCTCGTGGAAACCACTCTTTCCGGTAATTCATTCTGCTTAATTTTCAAACCAAACCTAAACTACTCCATATTCATGATATTCATCATCTAATAATACGAGGGTTTTTGTCGTTGTTGTTTCTAATTTATTTATGCAGTGAGAATGTGACGGCGTATGCGAAGTTGGTATCGGAATTGGACGAAAGGGTGAAAAGCATGGTGTTTGAAAGCTATGGGGTTGGAAATGCATTAAGAAGTCATATGGAATCAACAAAATATCTCATGAGAATGATAAAATACAGAGTCCCAAAGGAAAAAGAGATGAATTTGGGAGCATTTCCTCATACTGATAAGAGCTTTCTCACAATTCTTCATCAAAACGAAGTAAATGGTTTACAGATCAAAACGAGAGATAACAAATGGATTCAATATCATCCTTTTTCTTCTTCTTCAACCTCATCTTTCATTGTCATGGCTGGAGATGCTTTCTTTGTAAGTTCCATCTCATTTTATTAATCAATTTCTTCATCATCATCAAATTAAGATTAACATTAAGATTAAAGCCAAACTAATAATTAATGAATGGTTTAACTGTTGGCAGGCATGGAGCAATGGCAGGATCTACTCACCACCACACAGAGTGATAATGAGTGGAAACAAAGAGAGATATTCAGTTGGATTGTTTTCGTTCAACAATGGAATTGTTCAAATTCCCAAAGAATTGGTTGATGACAAACATCCACAATTGTTTAAACCCTTTGATCATCAAGACTATCTCCGTTTCTATAGCACCGAGAGAGGCCAAAATTCTCCTTGTGCCATCAAAGCTTATTGTGGAGTCGGAAATAGCCTCGTTGATACAGATACTCGGAGCCCTCTTCTCTTGCATTGA

mRNA sequence

ATGGGAACAAATATGAGTGATGAAGAACAACTCCCAGTTGTGAAGTTTTGTTCAGAGAATTTGAAGAGTGGAACTAAGGAATGGGTTTTGACAATGGAGAAAGTTCGGGAAGCTCTTGAGGAATACGGTTGTTTCGTAGCATTATACGACACCGTTTCAGCAGAAGTGAGTTCCAATGTGTTTGATTCTTTAAAAGAGTTGTTTGATGATGTTCCTTTGGAGAGGAAGCTTCAAAATGTTTCTGAGAAGCCATACCATGGCTACTTTGGTCAAAACCCTCTAATGCCCATCCATGAAAGTATGGGAATCGAACACCCCGTTCTCCCCACCAATATCAACTCCTTCACTAATCTCATGTGGCCTTCTCGTGGAAACCACTCTTTCCGTGAGAATGTGACGGCGTATGCGAAGTTGGTATCGGAATTGGACGAAAGGGTGAAAAGCATGGTGTTTGAAAGCTATGGGGTTGGAAATGCATTAAGAAGTCATATGGAATCAACAAAATATCTCATGAGAATGATAAAATACAGAGTCCCAAAGGAAAAAGAGATGAATTTGGGAGCATTTCCTCATACTGATAAGAGCTTTCTCACAATTCTTCATCAAAACGAAGTAAATGGTTTACAGATCAAAACGAGAGATAACAAATGGATTCAATATCATCCTTTTTCTTCTTCTTCAACCTCATCTTTCATTGTCATGGCTGGAGATGCTTTCTTTGCATGGAGCAATGGCAGGATCTACTCACCACCACACAGAGTGATAATGAGTGGAAACAAAGAGAGATATTCAGTTGGATTGTTTTCGTTCAACAATGGAATTGTTCAAATTCCCAAAGAATTGGTTGATGACAAACATCCACAATTGTTTAAACCCTTTGATCATCAAGACTATCTCCGTTTCTATAGCACCGAGAGAGGCCAAAATTCTCCTTGTGCCATCAAAGCTTATTGTGGAGTCGGAAATAGCCTCGTTGATACAGATACTCGGAGCCCTCTTCTCTTGCATTGA

Coding sequence (CDS)

ATGGGAACAAATATGAGTGATGAAGAACAACTCCCAGTTGTGAAGTTTTGTTCAGAGAATTTGAAGAGTGGAACTAAGGAATGGGTTTTGACAATGGAGAAAGTTCGGGAAGCTCTTGAGGAATACGGTTGTTTCGTAGCATTATACGACACCGTTTCAGCAGAAGTGAGTTCCAATGTGTTTGATTCTTTAAAAGAGTTGTTTGATGATGTTCCTTTGGAGAGGAAGCTTCAAAATGTTTCTGAGAAGCCATACCATGGCTACTTTGGTCAAAACCCTCTAATGCCCATCCATGAAAGTATGGGAATCGAACACCCCGTTCTCCCCACCAATATCAACTCCTTCACTAATCTCATGTGGCCTTCTCGTGGAAACCACTCTTTCCGTGAGAATGTGACGGCGTATGCGAAGTTGGTATCGGAATTGGACGAAAGGGTGAAAAGCATGGTGTTTGAAAGCTATGGGGTTGGAAATGCATTAAGAAGTCATATGGAATCAACAAAATATCTCATGAGAATGATAAAATACAGAGTCCCAAAGGAAAAAGAGATGAATTTGGGAGCATTTCCTCATACTGATAAGAGCTTTCTCACAATTCTTCATCAAAACGAAGTAAATGGTTTACAGATCAAAACGAGAGATAACAAATGGATTCAATATCATCCTTTTTCTTCTTCTTCAACCTCATCTTTCATTGTCATGGCTGGAGATGCTTTCTTTGCATGGAGCAATGGCAGGATCTACTCACCACCACACAGAGTGATAATGAGTGGAAACAAAGAGAGATATTCAGTTGGATTGTTTTCGTTCAACAATGGAATTGTTCAAATTCCCAAAGAATTGGTTGATGACAAACATCCACAATTGTTTAAACCCTTTGATCATCAAGACTATCTCCGTTTCTATAGCACCGAGAGAGGCCAAAATTCTCCTTGTGCCATCAAAGCTTATTGTGGAGTCGGAAATAGCCTCGTTGATACAGATACTCGGAGCCCTCTTCTCTTGCATTGA

Protein sequence

MGTNMSDEEQLPVVKFCSENLKSGTKEWVLTMEKVREALEEYGCFVALYDTVSAEVSSNVFDSLKELFDDVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPVLPTNINSFTNLMWPSRGNHSFRENVTAYAKLVSELDERVKSMVFESYGVGNALRSHMESTKYLMRMIKYRVPKEKEMNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFAWSNGRIYSPPHRVIMSGNKERYSVGLFSFNNGIVQIPKELVDDKHPQLFKPFDHQDYLRFYSTERGQNSPCAIKAYCGVGNSLVDTDTRSPLLLH*

Homology

[Close]

BLAST of Chy12G219410 vs. ExPASy Swiss-Prot
Match: Q945B6 (Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=3702 GN=AOP1.2 PE=2 SV=1)

HSP 1 Score: 279.6 bits (714), Expect = 4.5e-74
Identity = 136/315 (43.17%), Postives = 206/315 (65.40%), Query Frame = 0


Query: 10  QLPVVKFCSENLKSGTKEWVLTMEKVREALEEYGCFVALYDTVSAEVSSNVFDSLKELFD 69
           QLPV+ F  +NLK G+ +W      V +ALE+YGCF A +D +S E++ +VF+++++LF 
Sbjct: 13  QLPVIDFSDQNLKPGSSKWDEVKADVLKALEDYGCFEAFFDKLSVELNRSVFEAMEDLF- 72

Query: 70  DVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPVLPTNINSFTNLMWPSRGNHSFR 129
           ++P+  K +NVS KP+HGY   N    ++ES+GI+   +   +N FT  +WP  GN S  
Sbjct: 73  ELPIPTKQRNVSSKPFHGYLCHN----LYESLGIDDANVLEKVNDFTQQLWPDHGNKSIS 132

Query: 130 ENVTAYAKLVSELDERVKSMVFESYGVGNALRSHMESTKYLMRMIKYRVP-----KEKEM 189
           E +  +++ + ELD  V+ M+ ES+G+ N +  H+ ST YL R++KY  P      ++E 
Sbjct: 133 ETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTSPPDDDDDDEET 192

Query: 190 NLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFAWSN 249
            LG   HTDK+ +TILHQ +V+GL++KT+D+KWI+  P    S  S +VM GD+  A  N
Sbjct: 193 KLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKP----SQDSVLVMVGDSLCALLN 252

Query: 250 GRIYSPPHRVIMSGNKERYSVGLFSF--NNGIVQIPKELVDDKHPQLFKPFDHQDYLRFY 309
           GR++SP HRVIM+G K RYS GLFS      I+  P+ELVD +HP++FKPF++ D+L F+
Sbjct: 253 GRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFF 312

Query: 310 STERGQNSPCAIKAY 318
            TE G+ +  A+ A+
Sbjct: 313 QTEAGRIAQSALHAF 318

[Close]

BLAST of Chy12G219410 vs. ExPASy Swiss-Prot
Match: Q9ZTA3 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 277.7 bits (709), Expect = 1.7e-73
Identity = 136/316 (43.04%), Postives = 205/316 (64.87%), Query Frame = 0


Query: 10  QLPVVKFCSENLKSGTKEWVLTMEKVREALEEYGCFVALYDTVSAEVSSNVFDSLKELFD 69
           QLPV+ F  +NLK G+ +W      V +ALE+YGCF A +D +S E++ +VF+++++LF 
Sbjct: 13  QLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDLF- 72

Query: 70  DVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPVLPTNINSFTNLMWPSRGNHSFR 129
           ++P+  K +NVS KP+HGY   N    ++ES+GI    +   +N FT  +WP  GN S  
Sbjct: 73  ELPIPTKQRNVSSKPFHGYLCHN----LYESLGINDANVLEKVNDFTQQLWPDHGNKSIS 132

Query: 130 ENVTAYAKLVSELDERVKSMVFESYGVGNALRSHMESTKYLMRMIKYRVP------KEKE 189
           E +  +++ + ELD  V+ M+ ES+G+ N +  H+ ST YL R++KY  P       ++E
Sbjct: 133 ETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTSPPDDDDDDDEE 192

Query: 190 MNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFAWS 249
             LG   HTDK+ +TILHQ +V+GL++KT+D+KWI+  P    S  S +VM GD+  A  
Sbjct: 193 TKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKP----SQDSVLVMVGDSLCALL 252

Query: 250 NGRIYSPPHRVIMSGNKERYSVGLFSF--NNGIVQIPKELVDDKHPQLFKPFDHQDYLRF 309
           NGR++SP HRVIM+G K RYS GLFS      I+  P+ELVD +HP++FKPF++ D+L F
Sbjct: 253 NGRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHF 312

Query: 310 YSTERGQNSPCAIKAY 318
           + TE G+ +  A+ A+
Sbjct: 313 FQTEAGRIAQSALHAF 319

[Close]

BLAST of Chy12G219410 vs. ExPASy Swiss-Prot
Match: Q944Z5 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 268.9 bits (686), Expect = 8.0e-71
Identity = 132/305 (43.28%), Postives = 198/305 (64.92%), Query Frame = 0


Query: 10  QLPVVKFCSENLKSGTKEWVLTMEKVREALEEYGCFVALYDTVSAEVSSNVFDSLKELFD 69
           QLPV+ F  +NLK G+ +W      V +ALE+YGCF A +D +S E++ +VF+++++LF 
Sbjct: 6   QLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDLF- 65

Query: 70  DVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPVLPTNINSFTNLMWPSRGNHSFR 129
           ++P+  K +NVS K +HGY   N    ++ES+GI    +   +N FT  +WP  GN S  
Sbjct: 66  ELPIPTKQRNVSSKLFHGYLCHN----LYESLGINDANVLEKVNDFTQQLWPDHGNKSIS 125

Query: 130 ENVTAYAKLVSELDERVKSMVFESYGVGNALRSHMESTKYLMRMIKYRVP----KEKEMN 189
           E +  +++ + ELD  V+ M+ ES+G+   +  H+ ST YL R++KY  P     ++E  
Sbjct: 126 ETIHLFSEQLVELDLMVRRMIMESFGIEKYIDEHLNSTYYLTRLMKYTSPPDDDDDEETK 185

Query: 190 LGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFAWSNG 249
           LG   HTDK+ +TILHQ +V+GL++KT+D+KWI+  P    S  S +VM GD+  A  NG
Sbjct: 186 LGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKP----SQDSVLVMVGDSLCALLNG 245

Query: 250 RIYSPPHRVIMSGNKERYSVGLFSF--NNGIVQIPKELVDDKHPQLFKPFDHQDYLRFYS 309
           R++SP HRVIM+G K RYS GLFS      I+  P+ELVD +HP++FKPF++ D+L F+ 
Sbjct: 246 RLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFFQ 301

[Close]

BLAST of Chy12G219410 vs. ExPASy Swiss-Prot
Match: A0A0N9HQ36 (Deoxypodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=2-ODD PE=1 SV=1)

HSP 1 Score: 236.9 bits (603), Expect = 3.4e-61
Identity = 125/308 (40.58%), Postives = 180/308 (58.44%), Query Frame = 0


Query: 10  QLPVVKFCSENLKSGTKEWVLTMEKVREALEEYGCFVALYDTVSAEVSSNVFDSLKELFD 69
           +LPV+    +NLK GT  W     +VREALEEYGCF A+ D VS E+   V +   EL  
Sbjct: 8   RLPVIDLSMKNLKPGTTSWNSVRTQVREALEEYGCFEAVIDAVSPELQKAVCNKGHELL- 67

Query: 70  DVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPVLPTNINSFTNLMWPSRGNHSFR 129
           ++PLE K+ N ++  Y G+     +  ++E MG+        +  FTNLMWP  GN  F 
Sbjct: 68  NLPLETKMLNGNKPEYDGF---TSIPNLNEGMGVGRITDLEKVERFTNLMWP-EGNKDFC 127

Query: 130 ENVTAYAKLVSELDERVKSMVFESYGVGNALRSHMESTKYLMRMIKYRVPKEKEMNLGAF 189
           E V +Y K ++E+D  +K MVFES+G+     S  EST YL+  ++Y+ P +   +    
Sbjct: 128 ETVYSYGKRMAEVDHILKMMVFESFGMEKHFDSFCESTNYLLHFMRYQQPGKDGRSPALS 187

Query: 190 PHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFAWSNGRIYS 249
            H DKS LTI++QN+V GL+ +T+D +WI        +  + IV+ GD F AWSNGR++S
Sbjct: 188 LHKDKSILTIVNQNDVKGLEFETKDGEWI------LPTADNHIVLLGDCFMAWSNGRLHS 247

Query: 250 PPHRVIMSGNKERYSVGLFSFNNGIVQIPKELVDDKHPQLFKPFDHQDYLRFYSTERGQN 309
           P HRV +  N+ R S   FSF   I++ P ELVD++HP LF PF+  + L +  T+ G  
Sbjct: 248 PLHRVTLVANQARLSTSSFSFPKDIIETPAELVDEEHPLLFNPFEITELLAYCFTKEGAK 304

Query: 310 SPCAIKAY 318
           + C +K Y
Sbjct: 308 AVCDLKQY 304

[Close]

BLAST of Chy12G219410 vs. ExPASy Swiss-Prot
Match: Q945B5 (2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana OX=3702 GN=AOP2 PE=2 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 1.2e-53
Identity = 129/434 (29.72%), Postives = 201/434 (46.31%), Query Frame = 0


Query: 10  QLPVVKFCSENLKSGTKEWVLTMEKVREALEEYGCFVALYDTVSAEVSSNVFDSLKELFD 69
           QLP++    + L+ G+ +W      VR+ALE++GCF A YD VS E+  ++  +++ELF 
Sbjct: 7   QLPLINLADKTLEPGSSKWAEVRSDVRKALEDFGCFEASYDKVSLELQESIMKTMEELF- 66

Query: 70  DVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPVLPTNINSFTNLMWP-SRGNHSF 129
            +P+E K +NV  KPY GY   N L    ES+GI +  +  N+N FT  +WP   GN + 
Sbjct: 67  ALPVETKQRNVCPKPYVGYLNHNNL---SESLGISNANILENVNEFTQQLWPDGDGNENI 126

Query: 130 RENVTAYAKLVSELDERVKSMVFESYGVGNALRSHMESTKYLMRMIKYRVPKE------- 189
            + +  +A+ + E+D  V+ MV ES+G+   +  H++ST+Y MR++KY  P E       
Sbjct: 127 SKTIQLFAEKLVEIDVMVRRMVMESFGIEKYIDDHLKSTEYRMRLMKYIAPPEGDANTTV 186

Query: 190 ------------------------------------------------------------ 249
                                                                       
Sbjct: 187 DDYADLLAKLNIDGVEPNVGVKVNADISDDVNANPSVNAGVGANVNADTGVNDNLNVDAE 246

Query: 250 -------------------------------------------------KEMNLGAFPHT 309
                                                            +E  LG   HT
Sbjct: 247 ANGDANIAVGGGVNANTDLGVGVNVNSNVAVNAKTGATSGDDVEANDDNEEKKLGLPCHT 306

Query: 310 DKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFAWSNGRIYSPPH 324
           DK+  T+L Q+E+ GL++KT+D KWI+  P    S ++FIV+AGD+  A  NGRI +P H
Sbjct: 307 DKNLFTVLFQHEIEGLEVKTKDEKWIRVKP----SPNTFIVIAGDSLCALMNGRIRAPYH 366

[Close]

BLAST of Chy12G219410 vs. ExPASy TrEMBL
Match: A0A5D3BIZ7 (Putative 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G002910 PE=3 SV=1)

HSP 1 Score: 660.6 bits (1703), Expect = 3.5e-186
Identity = 316/336 (94.05%), Postives = 327/336 (97.32%), Query Frame = 0


Query: 1   MGTNMSDEEQLPVVKFCSENLKSGTKEWVLTMEKVREALEEYGCFVALYDTVSAEVSSNV 60
           M TNMSDE  LPVVKFCSENLKSGTKEWV TM+KVREALE+YGCFVALYD+VSAEVSSN+
Sbjct: 1   MATNMSDE--LPVVKFCSENLKSGTKEWVFTMKKVREALEDYGCFVALYDSVSAEVSSNM 60

Query: 61  FDSLKELFDDVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPVLPTNINSFTNLMW 120
           FDSLKELFDDVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHP+LPTNINSFTNLMW
Sbjct: 61  FDSLKELFDDVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMW 120

Query: 121 PSRGNHSFRENVTAYAKLVSELDERVKSMVFESYGVGNALRSHMESTKYLMRMIKYRVPK 180
           PSRGN SFRE+VT YAKLVSELDERVK MVFESYGVGNAL+SHMESTKYLMRMIKYRVPK
Sbjct: 121 PSRGNDSFRESVTTYAKLVSELDERVKRMVFESYGVGNALKSHMESTKYLMRMIKYRVPK 180

Query: 181 EKEMNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFF 240
           EKEMNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFF
Sbjct: 181 EKEMNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFF 240

Query: 241 AWSNGRIYSPPHRVIMSGNKERYSVGLFSFNNGIVQIPKELVDDKHPQLFKPFDHQDYLR 300
           AWSNGRIYSPPHRVIMSGN+ERYSVGLFSFNNGIVQIP+ELVDDKHPQLF PFDHQD+LR
Sbjct: 241 AWSNGRIYSPPHRVIMSGNRERYSVGLFSFNNGIVQIPEELVDDKHPQLFNPFDHQDFLR 300

Query: 301 FYSTERGQNSPCAIKAYCGVGNSLVDTDTRSPLLLH 337
           FYSTERGQNSPCAIKAYCGVGNSL+D DTRSPLLLH
Sbjct: 301 FYSTERGQNSPCAIKAYCGVGNSLIDMDTRSPLLLH 334

[Close]

BLAST of Chy12G219410 vs. ExPASy TrEMBL
Match: A0A6J1C712 (probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111008991 PE=3 SV=1)

HSP 1 Score: 540.0 bits (1390), Expect = 6.9e-150
Identity = 264/330 (80.00%), Postives = 290/330 (87.88%), Query Frame = 0


Query: 8   EEQLPVVKFCSENLKSGTKEWVLTMEKVREALEEYGCFVALYDTVSAEVSSNVFDSLKEL 67
           E QLP+VKF  ENL+S    WV T EKVREALEEYGCFVALYD+VS E+SSN+FDSLKEL
Sbjct: 3   EFQLPLVKFSEENLRS----WVSTREKVREALEEYGCFVALYDSVSMELSSNMFDSLKEL 62

Query: 68  FDDVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPVLPTNINSFTNLMWPSRGNHS 127
           F +VPLERKL+NVSEKPYHGYFGQNPLMPIHESMGIE P L +   SFTNLMWPS GN S
Sbjct: 63  F-EVPLERKLKNVSEKPYHGYFGQNPLMPIHESMGIEDPNLLSKTQSFTNLMWPSNGNKS 122

Query: 128 FRENVTAYAKLVSELDERVKSMVFESYGVGNALRSHMESTKYLMRMIKYRVPKEKEMNLG 187
           F ENV +YA+ ++ELDE VK MVFESYGV   L SHM+ST+YL+RMIKYRVPK+ EMN+G
Sbjct: 123 FCENVNSYARAIAELDETVKKMVFESYGVRKHLESHMKSTRYLLRMIKYRVPKQNEMNMG 182

Query: 188 AFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHP-FSSSSTSSFIVMAGDAFFAWSNGR 247
           AFPHTDKSFLTILHQN+VNGLQIKT+ NKW+QYHP  SSSSTSSFIVMAGDAFFAWSNGR
Sbjct: 183 AFPHTDKSFLTILHQNQVNGLQIKTKHNKWLQYHPSSSSSSTSSFIVMAGDAFFAWSNGR 242

Query: 248 IYSPPHRVIMSGNKERYSVGLFSFNNGIVQIPKELVDDKHPQLFKPFDHQDYLRFYSTER 307
           IYSPPH+VIMSG +ERYSVGLFSFN+GIVQIPKELVD+ HPQ FKPFDHQDYLRFYSTER
Sbjct: 243 IYSPPHKVIMSGKRERYSVGLFSFNDGIVQIPKELVDENHPQQFKPFDHQDYLRFYSTER 302

Query: 308 GQNSPCAIKAYCGVGNSLVDTDTRSPLLLH 337
           GQNSPCAIKAYCG+GN LV  DTR  +LLH
Sbjct: 303 GQNSPCAIKAYCGIGNGLV--DTRGCILLH 325

[Close]

BLAST of Chy12G219410 vs. ExPASy TrEMBL
Match: A0A6J1EAT7 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662 GN=LOC111431473 PE=3 SV=1)

HSP 1 Score: 501.1 bits (1289), Expect = 3.5e-138
Identity = 241/326 (73.93%), Postives = 280/326 (85.89%), Query Frame = 0


Query: 10  QLPVVKFCSENLKSGTKEWVLTMEKVREALEEYGCFVALYDTVSAEVSS-NVFDSLKELF 69
           ++PVV+F S NL+SG ++W  T EKVREALEEYGCFVALYD+VS E SS  + +++KE+F
Sbjct: 4   EIPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALYDSVSMEASSLKMMEAMKEVF 63

Query: 70  DDVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPVLPTNINSFTNLMWPSRGNHSF 129
            +VPLERK+QNVSEKP+HGYFGQNPLMPIHESMGIE P+LP+NI SF+NLMWPSR N  F
Sbjct: 64  -EVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPLF 123

Query: 130 RENVTAYAKLVSELDERVKSMVFESYGVGNALRSHMESTKYLMRMIKYRVPKEKEMNLGA 189
            EN+ +YA+LVS+L++ VK MVFESYG+ +   SH +STKYL+RMIKYRVP   EMNLGA
Sbjct: 124 CENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNLGA 183

Query: 190 FPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSS-SSTSSFIVMAGDAFFAWSNGRI 249
           FPHTDKSFLTILHQN VNGL IKTRDN+W++Y P S+ SS  SFIVMAGDAFFAWSNGRI
Sbjct: 184 FPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIVMAGDAFFAWSNGRI 243

Query: 250 YSPPHRVIMSGNKERYSVGLFSFNNGIVQIPKELVDDKHPQLFKPFDHQDYLRFYSTERG 309
           YS PHRV MSGN+ERYSVGLFSFN+GIVQIPKELVDDKHPQ FK FDHQDYLRFYSTE+G
Sbjct: 244 YSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTEKG 303

Query: 310 QNSPCAIKAYCGVGNSLVDTDTRSPL 334
           Q+SP AIKAYCGV N L+D+ T  P+
Sbjct: 304 QSSPSAIKAYCGVANGLLDSRTCIPM 328

[Close]

BLAST of Chy12G219410 vs. ExPASy TrEMBL
Match: A0A6J1E7C8 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662 GN=LOC111431472 PE=3 SV=1)

HSP 1 Score: 496.5 bits (1277), Expect = 8.7e-137
Identity = 239/326 (73.31%), Postives = 279/326 (85.58%), Query Frame = 0


Query: 10  QLPVVKFCSENLKSGTKEWVLTMEKVREALEEYGCFVALYDTVSAEVSS-NVFDSLKELF 69
           +LPVV+F S NL+SG ++W  T EKVREALEEYGCFVAL D+VS E SS  + ++++E+F
Sbjct: 4   ELPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEAMEEVF 63

Query: 70  DDVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPVLPTNINSFTNLMWPSRGNHSF 129
            +VPLERK+QNVSEKP+HGYFGQNPLMPIHESMGIE P+LP+NI SF+NLMWPSR N  F
Sbjct: 64  -EVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPLF 123

Query: 130 RENVTAYAKLVSELDERVKSMVFESYGVGNALRSHMESTKYLMRMIKYRVPKEKEMNLGA 189
            EN+ +YA+LVS+L++ VK MVFESYG+ +   SH +STKYL+RMIKYRVP   EMNLGA
Sbjct: 124 CENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNLGA 183

Query: 190 FPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSS-SSTSSFIVMAGDAFFAWSNGRI 249
           FPHTDKSFLTILHQN VNGL IKTRDN+W++Y P S+ SS  SFI+MAGDAFFAWSNGRI
Sbjct: 184 FPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIIMAGDAFFAWSNGRI 243

Query: 250 YSPPHRVIMSGNKERYSVGLFSFNNGIVQIPKELVDDKHPQLFKPFDHQDYLRFYSTERG 309
           YS PHRV MSGN+ERYSVGLFSFN+GIVQIPKELVDDKHPQ FK FDHQDYLRFYSTE+G
Sbjct: 244 YSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTEKG 303

Query: 310 QNSPCAIKAYCGVGNSLVDTDTRSPL 334
           Q+SP AIKAYCGV N L+D+ T  P+
Sbjct: 304 QSSPSAIKAYCGVANGLLDSRTCIPM 328

[Close]

BLAST of Chy12G219410 vs. ExPASy TrEMBL
Match: A0A6J1KFF7 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita maxima OX=3661 GN=LOC111495326 PE=3 SV=1)

HSP 1 Score: 492.3 bits (1266), Expect = 1.6e-135
Identity = 237/326 (72.70%), Postives = 278/326 (85.28%), Query Frame = 0


Query: 10  QLPVVKFCSENLKSGTKEWVLTMEKVREALEEYGCFVALYDTVSAEVSS-NVFDSLKELF 69
           ++PVV+F S N++SG ++W  T EKVREALEEYGCFVAL D+VS E SS  + +++KE+F
Sbjct: 4   EIPVVRFSSHNVQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSVKMMEAMKEVF 63

Query: 70  DDVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPVLPTNINSFTNLMWPSRGNHSF 129
            +VPLERK+QNVS+KP+HGYFGQNPLMPIHESMGIE P+LP+NI SF+NLMWPSR N  F
Sbjct: 64  -EVPLERKVQNVSQKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPLF 123

Query: 130 RENVTAYAKLVSELDERVKSMVFESYGVGNALRSHMESTKYLMRMIKYRVPKEKEMNLGA 189
             N+ +YA+LVS+L++ VK MVFESYG+ +   SH +STKYL+RMIKYRVP   EMNLGA
Sbjct: 124 CGNLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNLGA 183

Query: 190 FPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSS-SSTSSFIVMAGDAFFAWSNGRI 249
           FPHTDKSFLTILHQN VNGL IKTRDN+W++Y P S+ SS  SFIVMAGDAFFAWSNGRI
Sbjct: 184 FPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAQSSARSFIVMAGDAFFAWSNGRI 243

Query: 250 YSPPHRVIMSGNKERYSVGLFSFNNGIVQIPKELVDDKHPQLFKPFDHQDYLRFYSTERG 309
           YS PHRV MSGN+ERYSVGLFSFN+GIVQIPKELVDDKHPQ FK FDHQDYLRFYSTE+G
Sbjct: 244 YSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTEKG 303

Query: 310 QNSPCAIKAYCGVGNSLVDTDTRSPL 334
           Q+SP AIKAYCGV N L+D+ T  P+
Sbjct: 304 QSSPSAIKAYCGVANGLLDSRTCIPM 328

[Close]

BLAST of Chy12G219410 vs. NCBI nr
Match: XP_031738134.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis sativus] >KAE8652571.1 hypothetical protein Csa_014276 [Cucumis sativus])

HSP 1 Score: 672 bits (1735), Expect = 8.77e-244
Identity = 324/336 (96.43%), Postives = 331/336 (98.51%), Query Frame = 0


Query: 1   MGTNMSDEEQLPVVKFCSENLKSGTKEWVLTMEKVREALEEYGCFVALYDTVSAEVSSNV 60
           M TNMSDE QLPVVKFCS+NLKSGT+EWV T +KVREALEEYGCFVALYDTVSAEVSSN+
Sbjct: 4   MATNMSDE-QLPVVKFCSQNLKSGTEEWVSTTKKVREALEEYGCFVALYDTVSAEVSSNM 63

Query: 61  FDSLKELFDDVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPVLPTNINSFTNLMW 120
           FDSLKELFD+VPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHP+LPTNINSFTNLMW
Sbjct: 64  FDSLKELFDEVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMW 123

Query: 121 PSRGNHSFRENVTAYAKLVSELDERVKSMVFESYGVGNALRSHMESTKYLMRMIKYRVPK 180
           PSRGNHSFRENVTAYA LVSELD+RVKSMVFESYGVGNALRSHMESTKYLMRMIKYRVPK
Sbjct: 124 PSRGNHSFRENVTAYANLVSELDKRVKSMVFESYGVGNALRSHMESTKYLMRMIKYRVPK 183

Query: 181 EKEMNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFF 240
           EKEMNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFF
Sbjct: 184 EKEMNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFF 243

Query: 241 AWSNGRIYSPPHRVIMSGNKERYSVGLFSFNNGIVQIPKELVDDKHPQLFKPFDHQDYLR 300
           AWSNGRIYSPPHRVIMSGNKERYSVGLFSFNNGIVQIPKELVDDKHPQLFKPFDHQDYLR
Sbjct: 244 AWSNGRIYSPPHRVIMSGNKERYSVGLFSFNNGIVQIPKELVDDKHPQLFKPFDHQDYLR 303

Query: 301 FYSTERGQNSPCAIKAYCGVGNSLVDTDTRSPLLLH 336
           FYSTERGQNSPCAIKAYCGVGNSLVDTDTRSPLLLH
Sbjct: 304 FYSTERGQNSPCAIKAYCGVGNSLVDTDTRSPLLLH 338

[Close]

BLAST of Chy12G219410 vs. NCBI nr
Match: XP_004151475.3 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis sativus] >KAE8652570.1 hypothetical protein Csa_013560 [Cucumis sativus])

HSP 1 Score: 672 bits (1734), Expect = 1.25e-243
Identity = 323/336 (96.13%), Postives = 331/336 (98.51%), Query Frame = 0


Query: 1   MGTNMSDEEQLPVVKFCSENLKSGTKEWVLTMEKVREALEEYGCFVALYDTVSAEVSSNV 60
           M TNMSDE QLPVVKFCS+NLKSGT+EWV T +KVREALEEYGCFVALYDTVSAEVSSN+
Sbjct: 4   MATNMSDE-QLPVVKFCSQNLKSGTEEWVSTTKKVREALEEYGCFVALYDTVSAEVSSNM 63

Query: 61  FDSLKELFDDVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPVLPTNINSFTNLMW 120
           FDSLKELFD+VPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHP+LPTNINSFTNLMW
Sbjct: 64  FDSLKELFDEVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMW 123

Query: 121 PSRGNHSFRENVTAYAKLVSELDERVKSMVFESYGVGNALRSHMESTKYLMRMIKYRVPK 180
           PSRGNHSFRENVTAYA LVSELD+RVKSMVFESYGVGNALRSHMESTKYLMRMIKYRVPK
Sbjct: 124 PSRGNHSFRENVTAYANLVSELDKRVKSMVFESYGVGNALRSHMESTKYLMRMIKYRVPK 183

Query: 181 EKEMNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFF 240
           EKEMNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFF
Sbjct: 184 EKEMNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFF 243

Query: 241 AWSNGRIYSPPHRVIMSGNKERYSVGLFSFNNGIVQIPKELVDDKHPQLFKPFDHQDYLR 300
           AWSNGRIYSPPHRVIMSGNKERYSVGLFSFNNGIVQIPKELVDDKHPQLFKPFDHQDYLR
Sbjct: 244 AWSNGRIYSPPHRVIMSGNKERYSVGLFSFNNGIVQIPKELVDDKHPQLFKPFDHQDYLR 303

Query: 301 FYSTERGQNSPCAIKAYCGVGNSLVDTDTRSPLLLH 336
           FYSTERGQNSPCAIKAYCGVGNSL+DTDTRSPLLLH
Sbjct: 304 FYSTERGQNSPCAIKAYCGVGNSLIDTDTRSPLLLH 338

[Close]

BLAST of Chy12G219410 vs. NCBI nr
Match: TYJ99087.1 (putative 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis melo var. makuwa])

HSP 1 Score: 659 bits (1701), Expect = 1.15e-238
Identity = 316/336 (94.05%), Postives = 327/336 (97.32%), Query Frame = 0


Query: 1   MGTNMSDEEQLPVVKFCSENLKSGTKEWVLTMEKVREALEEYGCFVALYDTVSAEVSSNV 60
           M TNMSDE  LPVVKFCSENLKSGTKEWV TM+KVREALE+YGCFVALYD+VSAEVSSN+
Sbjct: 1   MATNMSDE--LPVVKFCSENLKSGTKEWVFTMKKVREALEDYGCFVALYDSVSAEVSSNM 60

Query: 61  FDSLKELFDDVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPVLPTNINSFTNLMW 120
           FDSLKELFDDVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHP+LPTNINSFTNLMW
Sbjct: 61  FDSLKELFDDVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMW 120

Query: 121 PSRGNHSFRENVTAYAKLVSELDERVKSMVFESYGVGNALRSHMESTKYLMRMIKYRVPK 180
           PSRGN SFRE+VT YAKLVSELDERVK MVFESYGVGNAL+SHMESTKYLMRMIKYRVPK
Sbjct: 121 PSRGNDSFRESVTTYAKLVSELDERVKRMVFESYGVGNALKSHMESTKYLMRMIKYRVPK 180

Query: 181 EKEMNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFF 240
           EKEMNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFF
Sbjct: 181 EKEMNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFF 240

Query: 241 AWSNGRIYSPPHRVIMSGNKERYSVGLFSFNNGIVQIPKELVDDKHPQLFKPFDHQDYLR 300
           AWSNGRIYSPPHRVIMSGN+ERYSVGLFSFNNGIVQIP+ELVDDKHPQLF PFDHQD+LR
Sbjct: 241 AWSNGRIYSPPHRVIMSGNRERYSVGLFSFNNGIVQIPEELVDDKHPQLFNPFDHQDFLR 300

Query: 301 FYSTERGQNSPCAIKAYCGVGNSLVDTDTRSPLLLH 336
           FYSTERGQNSPCAIKAYCGVGNSL+D DTRSPLLLH
Sbjct: 301 FYSTERGQNSPCAIKAYCGVGNSLIDMDTRSPLLLH 334

[Close]

BLAST of Chy12G219410 vs. NCBI nr
Match: XP_038893580.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Benincasa hispida])

HSP 1 Score: 615 bits (1585), Expect = 4.75e-221
Identity = 293/327 (89.60%), Postives = 310/327 (94.80%), Query Frame = 0


Query: 10  QLPVVKFCSENLKSGTKEWVLTMEKVREALEEYGCFVALYDTVSAEVSSNVFDSLKELFD 69
           Q+P+VKFCSENLKSG KEWVLT EKVREALEEYGCFVALYDTVS +VSSN+F SLK+LFD
Sbjct: 5   QIPIVKFCSENLKSGRKEWVLTREKVREALEEYGCFVALYDTVSMDVSSNMFHSLKQLFD 64

Query: 70  DVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPVLPTNINSFTNLMWPSRGNHSFR 129
            VPLERK+QNVSEKPYHGYFGQNPLMPIHESMGIEHP+LPTNI+SFTNLMWPS GN  F 
Sbjct: 65  -VPLERKIQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNIHSFTNLMWPSHGNRPFC 124

Query: 130 ENVTAYAKLVSELDERVKSMVFESYGVGNALRSHMESTKYLMRMIKYRVPKEKEMNLGAF 189
           ENVT YAKLVSELDERVKSMVFESYGVG  L++HMESTKYLMRMIKYRVPKEKEMNLGAF
Sbjct: 125 ENVTLYAKLVSELDERVKSMVFESYGVGKVLKTHMESTKYLMRMIKYRVPKEKEMNLGAF 184

Query: 190 PHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFAWSNGRIYS 249
           PHTDKSFLTILHQNEVNGLQIKTRDNKW+QYHP SSSSTSSFIVMAGDAFFAWSNGRIYS
Sbjct: 185 PHTDKSFLTILHQNEVNGLQIKTRDNKWLQYHPPSSSSTSSFIVMAGDAFFAWSNGRIYS 244

Query: 250 PPHRVIMSGNKERYSVGLFSFNNGIVQIPKELVDDKHPQLFKPFDHQDYLRFYSTERGQN 309
           PPHRV++S NKERYSVGLFSFN+GI+QIPKELVDDKHPQ F PFDHQDYLRFY+TERGQN
Sbjct: 245 PPHRVLVSANKERYSVGLFSFNDGIIQIPKELVDDKHPQQFNPFDHQDYLRFYTTERGQN 304

Query: 310 SPCAIKAYCGVGNSLVDTDTRSPLLLH 336
           SPCAIKAYCGVGNSLVDTD+RS LLLH
Sbjct: 305 SPCAIKAYCGVGNSLVDTDSRSTLLLH 330

[Close]

BLAST of Chy12G219410 vs. NCBI nr
Match: XP_038895877.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Benincasa hispida])

HSP 1 Score: 613 bits (1582), Expect = 1.31e-220
Identity = 292/327 (89.30%), Postives = 310/327 (94.80%), Query Frame = 0


Query: 10  QLPVVKFCSENLKSGTKEWVLTMEKVREALEEYGCFVALYDTVSAEVSSNVFDSLKELFD 69
           ++P+VKFCSENLKSG KEWVLT EKVREALEEYGCFVALYDTVS +VSSN+F SLK+LFD
Sbjct: 4   EIPIVKFCSENLKSGRKEWVLTREKVREALEEYGCFVALYDTVSMDVSSNMFHSLKQLFD 63

Query: 70  DVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPVLPTNINSFTNLMWPSRGNHSFR 129
            VPLERK+QNVSEKPYHGYFGQNPLMPIHESMGIEHP+LPTNI+SFTNLMWPS GN  F 
Sbjct: 64  -VPLERKIQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNIHSFTNLMWPSHGNRPFC 123

Query: 130 ENVTAYAKLVSELDERVKSMVFESYGVGNALRSHMESTKYLMRMIKYRVPKEKEMNLGAF 189
           ENVT YAKLVSELDERVKSMVFESYGVG  L++HMESTKYLMRMIKYRVPKEKEMNLGAF
Sbjct: 124 ENVTLYAKLVSELDERVKSMVFESYGVGKVLKTHMESTKYLMRMIKYRVPKEKEMNLGAF 183

Query: 190 PHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFAWSNGRIYS 249
           PHTDKSFLTILHQNEVNGLQIKTRDNKW+QYHP SSSSTSSFIVMAGDAFFAWSNGRIYS
Sbjct: 184 PHTDKSFLTILHQNEVNGLQIKTRDNKWLQYHPPSSSSTSSFIVMAGDAFFAWSNGRIYS 243

Query: 250 PPHRVIMSGNKERYSVGLFSFNNGIVQIPKELVDDKHPQLFKPFDHQDYLRFYSTERGQN 309
           PPHRV++S NKERYSVGLFSFN+GI+QIPKELVDDKHPQ F PFDHQDYLRFY+TERGQN
Sbjct: 244 PPHRVLVSANKERYSVGLFSFNDGIIQIPKELVDDKHPQQFNPFDHQDYLRFYTTERGQN 303

Query: 310 SPCAIKAYCGVGNSLVDTDTRSPLLLH 336
           SPCAIKAYCGVGNSLVDTD+RS LLLH
Sbjct: 304 SPCAIKAYCGVGNSLVDTDSRSTLLLH 329

[Close]

BLAST of Chy12G219410 vs. TAIR 10
Match: AT1G52820.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 314.3 bits (804), Expect = 1.2e-85
Identity = 147/313 (46.96%), Postives = 220/313 (70.29%), Query Frame = 0


Query: 10  QLPVVKFCSENLKSGTKEWVLTMEKVREALEEYGCFVALYDTVSAEVSSNVFDSLKELFD 69
           +LPV+ F ++NLK G  EW LT   V++AL++YG F A +D +  E+  +VF +L+ELF 
Sbjct: 11  RLPVIDFSNKNLKPGEPEWDLTRADVQKALQDYGYFEASFDRIPFELRKSVFGALEELF- 70

Query: 70  DVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPVLPTNINSFTNLMWPSRGNHSFR 129
           D+PL+ KL+NVS+KP+HGY GQ P++P++ESMGI+   +   +++FT  +WP +GN SF 
Sbjct: 71  DLPLQTKLRNVSKKPFHGYVGQYPMVPLYESMGIDDSDIAEKVDAFTEKLWP-QGNISFS 130

Query: 130 ENVTAYAKLVSELDERVKSMVFESYGVGNALRSHMESTKYLMRMIKYRVPKEKEMNLGAF 189
             + +++K +SELD  ++ M+ ES+G+   +  H+ ST YL+R++KY+ P  +E  +G  
Sbjct: 131 TTIQSFSKKLSELDITIRRMIMESFGLDKYIDEHLHSTNYLLRVMKYKGPDTEETKVGLN 190

Query: 190 PHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFAWSNGRIYS 249
            HTDK+ +TIL+QN V GL+++T+D  WI+  P    +  SF VM GD+ +A  NGR++S
Sbjct: 191 AHTDKNIVTILYQNHVEGLEVQTKDKNWIKVKP----TQDSFTVMIGDSLYALLNGRLHS 250

Query: 250 PPHRVIMSGNKERYSVGLFSFNNG--IVQIPKELVDDKHPQLFKPFDHQDYLRFYSTERG 309
           P HRV+M+G + RYS+GLFS      IV  P ELVD++HP+LFKPFDH ++L+FY TE G
Sbjct: 251 PYHRVMMTGTETRYSLGLFSIPKAGHIVSSPDELVDEEHPRLFKPFDHVEFLQFYYTEAG 310

Query: 310 QNSPCAIKAYCGV 321
           Q S  A+K YCG+
Sbjct: 311 QRSQSALKTYCGI 317

[Close]

BLAST of Chy12G219410 vs. TAIR 10
Match: AT1G52790.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 314.3 bits (804), Expect = 1.2e-85
Identity = 152/311 (48.87%), Postives = 212/311 (68.17%), Query Frame = 0


Query: 10  QLPVVKFCSENLKSGTKEWVLTMEKVREALEEYGCFVA-LYDTVSAEVSSNVFDSLKELF 69
           ++P + F  E+LK GTK W  T E +R+ALEEYGCF+  L D    ++   VF SL +LF
Sbjct: 4   KIPTLDFSREDLKPGTKYWESTRENIRQALEEYGCFIIDLKDKTPLDLLDRVFGSLVDLF 63

Query: 70  DDVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPVLPTNINSFTNLMWPSRGNHSF 129
            D+P + K++N  +KP +GY GQ P +P+HES+GI++        SFT LMWP +GN  F
Sbjct: 64  -DLPTQTKMKNKYDKPLNGYVGQIPALPLHESLGIDNATSLEATRSFTGLMWP-QGNEHF 123

Query: 130 RENVTAYAKLVSELDERVKSMVFESYGVGNALRSHMESTKYLMRMIKYRVPKEKEMNLGA 189
            E +  YA+  +ELD+ V  MVF+SY V      ++EST YL+R++K R P  +   LG 
Sbjct: 124 SECLYKYAEFAAELDQMVTRMVFQSYNVEKYYDPYIESTTYLLRVLKNRAPNNENPTLGF 183

Query: 190 FPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFAWSNGRIY 249
             HTDKSF TILHQ++VNGL+++TR+ + I     + SS S F+V+AGDA  AWSN R++
Sbjct: 184 VTHTDKSFTTILHQDQVNGLEMETREGERIN---INLSSPSLFMVVAGDALMAWSNDRVW 243

Query: 250 SPPHRVIMSGNKERYSVGLFSFNNGIVQIPKELVDDKHPQLFKPFDHQDYLRFYSTERGQ 309
           SP H+V++SG  +RYS+G+F+FNNG +Q+P+EL+D +HP ++KPFDH   LRFY T+ G 
Sbjct: 244 SPRHQVLVSGETDRYSLGMFAFNNGTLQVPEELIDHQHPLMYKPFDHLGLLRFYRTDTGY 303

Query: 310 NSPCAIKAYCG 320
            S C IKAYCG
Sbjct: 304 KSECPIKAYCG 309

[Close]

BLAST of Chy12G219410 vs. TAIR 10
Match: AT1G52800.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 296.6 bits (758), Expect = 2.5e-80
Identity = 144/313 (46.01%), Postives = 208/313 (66.45%), Query Frame = 0


Query: 10  QLPVVKFCS-ENLKSGTKEWVLTMEKVREALEEYGCFVALYDTVSAEVSSNVFDSLKELF 69
           ++P++   S + LK  T  W     +  EALEEYGCF+A+YD V+ ++  ++F + +ELF
Sbjct: 9   KVPILDLTSQQELKPNTSTWRSVSREACEALEEYGCFLAVYDGVTQQLDDSIFAAAEELF 68

Query: 70  DDVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPVLPTNINSFTNLMWPSRGNHSF 129
            D+P E K +NV+EKPYHGY GQ P++P+HE +G+++         FT+LMWP +GN  F
Sbjct: 69  -DLPTETKKKNVNEKPYHGYVGQMPVIPLHEGLGVDYVTNKEIAQRFTHLMWP-QGNDRF 128

Query: 130 RENVTAYAKLVSELDERVKSMVFESYGVGNALRSHMESTKYLMRMIKYRVPKEKEMNLGA 189
              V  ++  V+ELD  V  M+FE+YGV     SH+ S  YL++ +KY  P E  +++ A
Sbjct: 129 CNTVHTFSNAVAELDRLVVRMIFENYGVEKHYESHVGSKTYLLKFLKYLAPPE-SISMPA 188

Query: 190 FP-HTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFAWSNGRI 249
           FP HTDK+FL+ILHQN+VNGL++K++D +WI           S++VMAGD    WSN RI
Sbjct: 189 FPQHTDKTFLSILHQNDVNGLEVKSKDGEWISL----QLPPKSYVVMAGDISMGWSNDRI 248

Query: 250 YSPPHRVIMSGNKERYSVGLFSFNNGIVQIPKELVDDKHPQLFKPFDHQDYLRFYSTERG 309
            S  HRV M G+K RY++GLFSF   +V IP+ELVDDKHP ++KPFD+   + FY+T+ G
Sbjct: 249 RSCEHRVTMEGDKTRYTLGLFSFLTDLVSIPEELVDDKHPLMYKPFDNIALINFYTTKEG 308

Query: 310 QNSPCAIKAYCGV 321
           + +   +KAYCG+
Sbjct: 309 REANSTLKAYCGI 314

[Close]

BLAST of Chy12G219410 vs. TAIR 10
Match: AT4G03070.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 277.7 bits (709), Expect = 1.2e-74
Identity = 136/316 (43.04%), Postives = 205/316 (64.87%), Query Frame = 0


Query: 10  QLPVVKFCSENLKSGTKEWVLTMEKVREALEEYGCFVALYDTVSAEVSSNVFDSLKELFD 69
           QLPV+ F  +NLK G+ +W      V +ALE+YGCF A +D +S E++ +VF+++++LF 
Sbjct: 13  QLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDLF- 72

Query: 70  DVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPVLPTNINSFTNLMWPSRGNHSFR 129
           ++P+  K +NVS KP+HGY   N    ++ES+GI    +   +N FT  +WP  GN S  
Sbjct: 73  ELPIPTKQRNVSSKPFHGYLCHN----LYESLGINDANVLEKVNDFTQQLWPDHGNKSIS 132

Query: 130 ENVTAYAKLVSELDERVKSMVFESYGVGNALRSHMESTKYLMRMIKYRVP------KEKE 189
           E +  +++ + ELD  V+ M+ ES+G+ N +  H+ ST YL R++KY  P       ++E
Sbjct: 133 ETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTSPPDDDDDDDEE 192

Query: 190 MNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFAWS 249
             LG   HTDK+ +TILHQ +V+GL++KT+D+KWI+  P    S  S +VM GD+  A  
Sbjct: 193 TKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKP----SQDSVLVMVGDSLCALL 252

Query: 250 NGRIYSPPHRVIMSGNKERYSVGLFSF--NNGIVQIPKELVDDKHPQLFKPFDHQDYLRF 309
           NGR++SP HRVIM+G K RYS GLFS      I+  P+ELVD +HP++FKPF++ D+L F
Sbjct: 253 NGRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHF 312

Query: 310 YSTERGQNSPCAIKAY 318
           + TE G+ +  A+ A+
Sbjct: 313 FQTEAGRIAQSALHAF 319

[Close]

BLAST of Chy12G219410 vs. TAIR 10
Match: AT1G80320.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 250.4 bits (638), Expect = 2.1e-66
Identity = 129/320 (40.31%), Postives = 196/320 (61.25%), Query Frame = 0


Query: 7   DEEQLPVVKFCSENLKSGTKEWVLTMEKVREALEEYGCFVALYDTVSAEVSSNVFDSLKE 66
           +E+ LP++ F S+ L  GT  W+ T + VR A+E  G FVA +  VS+++  N+   +KE
Sbjct: 5   EEQCLPILDFSSDKLVRGTSHWITTRDDVRRAMEGQGWFVAEFSGVSSDLRDNLLAGMKE 64

Query: 67  LFDDVPLERKLQNVSEKPYHGYFGQ-NPLMPIHESMGIEHPVLPTNINSFTNLMWPSRGN 126
           ++  +P + K++N + K  HGY         IHES+GI++         F+ L+WP +GN
Sbjct: 65  MY-YLPDQIKIKNENHKASHGYMSMVVDDYRIHESLGIDYATELQACKDFSKLLWP-QGN 124

Query: 127 HSFRENVTAYAKLVSELDERVKSMVFESYGVGNALR--SHMESTKYLMRMIKYRVPKEKE 186
             F +    YA  ++ELD+ V  M++ESYG+       SH EST+YL+RM+ YR  +  E
Sbjct: 125 DPFCQTTHMYAMTMAELDQTVMRMLYESYGMDEKKHSVSHSESTRYLLRMLSYRRQQNGE 184

Query: 187 MNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFAWS 246
            N G   HTDKSF++ILHQN V GLQ+KT   +W+ ++P    S + F+V++G    AWS
Sbjct: 185 ANTGFVSHTDKSFMSILHQNHVGGLQLKTMTGQWVGFNP----SPTRFVVLSGMGLTAWS 244

Query: 247 NGRIYSPPHRVIMSGNKERYSVGLFSFNNGIVQIPKELVDDKHPQLFKPFDHQDYLRFYS 306
           N RI +  H+V+MS ++ RYS+G FSF+ G ++ P+ELVDD+HP  + PF+H   LRFY 
Sbjct: 245 NDRIKACYHKVVMSADEIRYSLGFFSFHKGTIRTPEELVDDQHPLRYNPFEHDGLLRFYE 304

Query: 307 T---ERGQNSPCAIKAYCGV 321
           +      ++S   ++ YCGV
Sbjct: 305 SYINSLKKSSEDLLQIYCGV 318

The following BLAST results are available for this feature:

BLAST of Chy12G219410 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q945B6	4.5e-74	43.17	Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=...	[more]
Q9ZTA3	1.7e-73	43.04	Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=37...	[more]
Q944Z5	8.0e-71	43.28	Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis tha...	[more]
A0A0N9HQ36	3.4e-61	40.58	Deoxypodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=2-ODD PE=...	[more]
Q945B5	1.2e-53	29.72	2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana OX=3702 GN=AOP...	[more]

BLAST of Chy12G219410 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3BIZ7	3.5e-186	94.05	Putative 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucumis melo var. makuwa O...	[more]
A0A6J1C712	6.9e-150	80.00	probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 OS=Momordica chara...	[more]
A0A6J1EAT7	3.5e-138	73.93	probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662...	[more]
A0A6J1E7C8	8.7e-137	73.31	probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662...	[more]
A0A6J1KFF7	1.6e-135	72.70	probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita maxima OX=3661 G...	[more]

BLAST of Chy12G219410 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_031738134.1	8.77e-244	96.43	probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis sativus] >KAE8652571...	[more]
XP_004151475.3	1.25e-243	96.13	probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis sativus] >KAE8652570...	[more]
TYJ99087.1	1.15e-238	94.05	putative 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis melo var. makuwa]	[more]
XP_038893580.1	4.75e-221	89.60	probable 2-oxoglutarate-dependent dioxygenase AOP1 [Benincasa hispida]	[more]
XP_038895877.1	1.31e-220	89.30	probable 2-oxoglutarate-dependent dioxygenase AOP1 [Benincasa hispida]	[more]

BLAST of Chy12G219410 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G52820.1	1.2e-85	46.96	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G52790.1	1.2e-85	48.87	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G52800.1	2.5e-80	46.01	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT4G03070.1	1.2e-74	43.04	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G80320.1	2.1e-66	40.31	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 26..90 e-value: 1.4E-7 score: 32.3
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 2..301 e-value: 5.3E-56 score: 192.5
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 176..270 e-value: 3.1E-18 score: 66.1
None	No IPR available	PANTHER	PTHR47990:SF17	2-OXOGLUTARATE-DEPENDENT DIOXYGENASE AOP3-LIKE	coord: 6..319
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 6..319
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 7..307
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 167..273 score: 10.992504

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy12G219410.1	Chy12G219410.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0046872	metal ion binding