Chy12G218220 (gene) Cucumber (hystrix) v1

Overview


Name	Chy12G218220
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	cytochrome P450 71A1-like
Location	chrH12: 16943230 .. 16945650 (+)
RNA-Seq Expression	Chy12G218220
Synteny	Chy12G218220

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTCCTCAAACTAGGCCAAACTCCAACTCTAGTGGTTTCCTCACCAATAGTGGCTAGAGAAATTATGAAGACCCATGACCTTAAATTCTCCAATAGACCCAAAACCACAGCCCCAAATCTCATGTTCTATAGTTGCCAAGATGTGGGTTTTGCCCCATATGGTGATTATTGGAAAAAAGCCAAAAAAATGTGTGTTGTTGAGCTTTTTAGCCACAAAAGAGTTGAATCTTTTCAATATGTTAGAGATGAAGAAATTGATATACTTATTAATAAAGTTCATAAGGGAGCTAATTGTAGTGAGAGTTTGAATCTTACCCAATTATTGTTTCAAACCTCAAACGATATAGTGTCAAGATGTGTTCTGGGGAAGAAATTTGAGGATGAAAATGGGAAGAATGAATTTAGAGACTTGTACACAAGAAGAGTAATTGAGCTACTCTCGGCTTTTTGTGTGGGGGATTTCTTTCCTAATTTTGAATGGGTTGATGTTATTAGAGGATTGGTTGGAGAAATGAAAAAGGTTTCTAAGATAATTGATGGATTCTTTGATATGGTGATTGAAGATCACGTCATAAAGTTGAGATCAAGTGATAGATGTGATGATAAAAAGGATTTTGTGGATATCATGTTGCAGCAGCTCAATGATGAAGATTACACGTTTCATGACCATTTCACTAGAGACAACCTCAAAGGCATCTTAATGGTTTCTTTTCTTCATCTCTCTAATTTTTTTTGTGATACTTTTATTAGATTACATCTATTTTTTTTCGATCACTAAAAAATATTACATATAGAAAAGAGAGAACTGCATTTTCAATCCTTAGTTTTTCAAGAATAGGAGCATTTTGGTCAATGAAATTTCAAAATATACCTTTTTGGTTTGAATTTTTCTTAAGTTTTCAATATATATATTTTTAGTCGAATTTTTTTATCCATGTTCTTCAAAATTTGGAGATGAAAAGAACTTTAGGATACCGAACATAGTCAAAAAGAGGAAAAGAAAGTGAAACTTTATTAAATGCTTACTTTATATATTCTAATAGTGTGGAATCGCTAGTTTCAATTGAAAAAAAAAACCAAAAAGCCATAGTTAAAATCAAAACAAATTATCTCCTATTAAAAACTATAAGATATCTATAAATATATGTACTATTTTAATTTGTATTTGGAAAACGTGCGATCATACCATAACTACTGCACCATATTCCATTAGAACTCTATGTTGTTAAATGTGTTAGATAAGACTAGTTGGAAAAATTGGTGCTTACCGATATTTATAAATTTTAAAATATCTACTAATTTTATTCACAATTCACAATTATTATTATTATTAATTTTTTTTTTTGTATATAAAGGTGGACCATGCATCCATTATCTCCTCTATCGTTGTAATTGAAAAACGAAGACAAATTTTCCACCACCATTCTTATAAATCTCTCGTGTTTCTCTCTTCTTTTTTGTTTTTAAAATGTTCAATCATATGTCAAAATTTTCTTACTCATTTTTTATAATAATTAAAAAAAATTGTATATTATGAAAATGTGATACATAAAGATGCATGTTATTAAAATAGACAAAAATTTCTCTAATTTGTGTTTATTTTCCTCTCTTAATTGTAAGATTTCAACAAATGGCAACCCAAAATGTCGCAACACACAATAATAAGTATTAGATGTACTGAATTTGAAAATCTAGATATTCTATAAATTAAATATTTTTTAATTTCTCTTCCCTACTATGTTAGACTATATAAAGCTAACTGTGAATCAAATTGTAATGAATTTGAATTTTAACGTTTCAAATGCAGGACATGTTCATAGGAGGGACAGACTCAACAGCAACAATATTAGAATGGACAATGGCAGAGTTGTTAAGAAATCCAAACACCATGAAGAAAGTCCAACAAGAAATAAGAACAATAGTTGGAAAAAACAAGAAAAAAATTGAAATAAAGGACATTAACAAAATGGAGTACATGAAATGTGTAATAAAAGAATCTCTAAGATTACACCCACCAGTTCCTCTACTTGTACCTCGAGAAACAACAGACATGGTTGATATCGAAGGGTACCACGTGGGAGCAGGAACAAGTGTGCTTGTGAATGTTTGGGCAATTCAAAGAGACCCCAAAATATGGGAAAACCCAAACCAGTTCATCCCAGAGAGATTTATGGAGGAAAATAAATCAATTGATTTTAAAGGATCAGATTTTGAGTTGGTTCCATTTGGAAGTGGAAGAAGGAAATGCCCTGGAATAGGGTTTGGAATGGCTTCTTCTGAATGTGTGTTGGTCAATCTGTTGTATTGGTTTGATTGGAATATGGTTGAGGATATGAAAGGAGAATTGATGGATATGACTGAAGAAAATGGAAGCACTGTTCATAAGAAAATCCCTCTGTCTCATCCCATTACCGTATAA

mRNA sequence

ATGCTCCTCAAACTAGGCCAAACTCCAACTCTAGTGGTTTCCTCACCAATAGTGGCTAGAGAAATTATGAAGACCCATGACCTTAAATTCTCCAATAGACCCAAAACCACAGCCCCAAATCTCATGTTCTATAGTTGCCAAGATGTGGGTTTTGCCCCATATGGTGATTATTGGAAAAAAGCCAAAAAAATGTGTGTTGTTGAGCTTTTTAGCCACAAAAGAGTTGAATCTTTTCAATATGTTAGAGATGAAGAAATTGATATACTTATTAATAAAGTTCATAAGGGAGCTAATTGTAGTGAGAGTTTGAATCTTACCCAATTATTGTTTCAAACCTCAAACGATATAGTGTCAAGATGTGTTCTGGGGAAGAAATTTGAGGATGAAAATGGGAAGAATGAATTTAGAGACTTGTACACAAGAAGAGTAATTGAGCTACTCTCGGCTTTTTGTGTGGGGGATTTCTTTCCTAATTTTGAATGGGTTGATGTTATTAGAGGATTGGTTGGAGAAATGAAAAAGGTTTCTAAGATAATTGATGGATTCTTTGATATGGTGATTGAAGATCACGTCATAAAGTTGAGATCAAGTGATAGATGTGATGATAAAAAGGATTTTGTGGATATCATGTTGCAGCAGCTCAATGATGAAGATTACACGTTTCATGACCATTTCACTAGAGACAACCTCAAAGGCATCTTAATGGACATGTTCATAGGAGGGACAGACTCAACAGCAACAATATTAGAATGGACAATGGCAGAGTTGTTAAGAAATCCAAACACCATGAAGAAAGTCCAACAAGAAATAAGAACAATAGTTGGAAAAAACAAGAAAAAAATTGAAATAAAGGACATTAACAAAATGGAGTACATGAAATGTGTAATAAAAGAATCTCTAAGATTACACCCACCAGTTCCTCTACTTGTACCTCGAGAAACAACAGACATGGTTGATATCGAAGGGTACCACGTGGGAGCAGGAACAAGTGTGCTTGTGAATGTTTGGGCAATTCAAAGAGACCCCAAAATATGGGAAAACCCAAACCAGTTCATCCCAGAGAGATTTATGGAGGAAAATAAATCAATTGATTTTAAAGGATCAGATTTTGAGTTGGTTCCATTTGGAAGTGGAAGAAGGAAATGCCCTGGAATAGGGTTTGGAATGGCTTCTTCTGAATGTGTGTTGGTCAATCTGTTGTATTGGTTTGATTGGAATATGGTTGAGGATATGAAAGGAGAATTGATGGATATGACTGAAGAAAATGGAAGCACTGTTCATAAGAAAATCCCTCTGTCTCATCCCATTACCGTATAA

Coding sequence (CDS)

ATGCTCCTCAAACTAGGCCAAACTCCAACTCTAGTGGTTTCCTCACCAATAGTGGCTAGAGAAATTATGAAGACCCATGACCTTAAATTCTCCAATAGACCCAAAACCACAGCCCCAAATCTCATGTTCTATAGTTGCCAAGATGTGGGTTTTGCCCCATATGGTGATTATTGGAAAAAAGCCAAAAAAATGTGTGTTGTTGAGCTTTTTAGCCACAAAAGAGTTGAATCTTTTCAATATGTTAGAGATGAAGAAATTGATATACTTATTAATAAAGTTCATAAGGGAGCTAATTGTAGTGAGAGTTTGAATCTTACCCAATTATTGTTTCAAACCTCAAACGATATAGTGTCAAGATGTGTTCTGGGGAAGAAATTTGAGGATGAAAATGGGAAGAATGAATTTAGAGACTTGTACACAAGAAGAGTAATTGAGCTACTCTCGGCTTTTTGTGTGGGGGATTTCTTTCCTAATTTTGAATGGGTTGATGTTATTAGAGGATTGGTTGGAGAAATGAAAAAGGTTTCTAAGATAATTGATGGATTCTTTGATATGGTGATTGAAGATCACGTCATAAAGTTGAGATCAAGTGATAGATGTGATGATAAAAAGGATTTTGTGGATATCATGTTGCAGCAGCTCAATGATGAAGATTACACGTTTCATGACCATTTCACTAGAGACAACCTCAAAGGCATCTTAATGGACATGTTCATAGGAGGGACAGACTCAACAGCAACAATATTAGAATGGACAATGGCAGAGTTGTTAAGAAATCCAAACACCATGAAGAAAGTCCAACAAGAAATAAGAACAATAGTTGGAAAAAACAAGAAAAAAATTGAAATAAAGGACATTAACAAAATGGAGTACATGAAATGTGTAATAAAAGAATCTCTAAGATTACACCCACCAGTTCCTCTACTTGTACCTCGAGAAACAACAGACATGGTTGATATCGAAGGGTACCACGTGGGAGCAGGAACAAGTGTGCTTGTGAATGTTTGGGCAATTCAAAGAGACCCCAAAATATGGGAAAACCCAAACCAGTTCATCCCAGAGAGATTTATGGAGGAAAATAAATCAATTGATTTTAAAGGATCAGATTTTGAGTTGGTTCCATTTGGAAGTGGAAGAAGGAAATGCCCTGGAATAGGGTTTGGAATGGCTTCTTCTGAATGTGTGTTGGTCAATCTGTTGTATTGGTTTGATTGGAATATGGTTGAGGATATGAAAGGAGAATTGATGGATATGACTGAAGAAAATGGAAGCACTGTTCATAAGAAAATCCCTCTGTCTCATCCCATTACCGTATAA

Protein sequence

MLLKLGQTPTLVVSSPIVAREIMKTHDLKFSNRPKTTAPNLMFYSCQDVGFAPYGDYWKKAKKMCVVELFSHKRVESFQYVRDEEIDILINKVHKGANCSESLNLTQLLFQTSNDIVSRCVLGKKFEDENGKNEFRDLYTRRVIELLSAFCVGDFFPNFEWVDVIRGLVGEMKKVSKIIDGFFDMVIEDHVIKLRSSDRCDDKKDFVDIMLQQLNDEDYTFHDHFTRDNLKGILMDMFIGGTDSTATILEWTMAELLRNPNTMKKVQQEIRTIVGKNKKKIEIKDINKMEYMKCVIKESLRLHPPVPLLVPRETTDMVDIEGYHVGAGTSVLVNVWAIQRDPKIWENPNQFIPERFMEENKSIDFKGSDFELVPFGSGRRKCPGIGFGMASSECVLVNLLYWFDWNMVEDMKGELMDMTEENGSTVHKKIPLSHPITV*

Homology

[Close]

BLAST of Chy12G218220 vs. ExPASy Swiss-Prot
Match: P24465 (Cytochrome P450 71A1 OS=Persea americana OX=3435 GN=CYP71A1 PE=1 SV=2)

HSP 1 Score: 449.1 bits (1154), Expect = 5.6e-125
Identity = 216/435 (49.66%), Postives = 309/435 (71.03%), Query Frame = 0


Query: 1   MLLKLGQTPTLVVSSPIVAREIMKTHDLKFSNRPKTTAPNLMFYSCQDVGFAPYGDYWKK 60
           +LL LG  PTL+VS+  +A EI+KTHDL F++RP TTA   +FY C DV F+PYG+YW++
Sbjct: 66  ILLHLGHIPTLIVSTAEIAEEILKTHDLIFASRPSTTAARRIFYDCTDVAFSPYGEYWRQ 125

Query: 61  AKKMCVVELFSHKRVESFQYVRDEEIDILINKVHKGANCSESLNLTQLLFQTSNDIVSRC 120
            +K+CV+EL S KRV S++ +R+EE+ +++ ++ +  +  E++NL++LL   S+  ++R 
Sbjct: 126 VRKICVLELLSIKRVNSYRSIREEEVGLMMERISQSCSTGEAVNLSELLLLLSSGTITRV 185

Query: 121 VLGKKFE-DENGKNEFRDLYTRRVIELLSAFCVGDFFPNFEWVDVIRGLVGEMKKVSKII 180
             GKK+E +E  KN+F DL T  +  L+ AF VGD+FP+F WVDV+ G+   +K+    +
Sbjct: 186 AFGKKYEGEEERKNKFADLAT-ELTTLMGAFFVGDYFPSFAWVDVLTGMDARLKRNHGEL 245

Query: 181 DGFFDMVIEDHVI--KLRSSDRCDDKKDFVDIMLQQLNDEDYTFHDHFTRDNLKGILMDM 240
           D F D VI+DH++  K   SD   ++KD VD++L     +D +   H  R+NLK +++DM
Sbjct: 246 DAFVDHVIDDHLLSRKANGSDGV-EQKDLVDVLLHL--QKDSSLGVHLNRNNLKAVILDM 305

Query: 241 FIGGTDSTATILEWTMAELLRNPNTMKKVQQEIRTIVGKNKKKIEIKDINKMEYMKCVIK 300
           F GGTD+TA  LEW MAEL+++P+ M+K QQE+R +VGK K K+E +D++++ Y+K +IK
Sbjct: 306 FSGGTDTTAVTLEWAMAELIKHPDVMEKAQQEVRRVVGK-KAKVEEEDLHQLHYLKLIIK 365

Query: 301 ESLRLHPPVPLLVPRETTDMVDIEGYHVGAGTSVLVNVWAIQRDPKIWENPNQFIPERFM 360
           E+LRLHP  PLLVPRE+T  V I GYH+ A T V +N WAI RDPK WEN  +F+PERF+
Sbjct: 366 ETLRLHPVAPLLVPRESTRDVVIRGYHIPAKTRVFINAWAIGRDPKSWENAEEFLPERFV 425

Query: 361 EENKSIDFKGSDFELVPFGSGRRKCPGIGFGMASSECVLVNLLYWFDWNMVEDMKGELMD 420
             N S+DFKG DF+L+PFG+GRR CPGI FG++S E  L NLLYWF+W +  D+  E +D
Sbjct: 426 --NNSVDFKGQDFQLIPFGAGRRGCPGIAFGISSVEISLANLLYWFNWELPGDLTKEDLD 485

Query: 421 MTEENGSTVHKKIPL 433
           M+E  G TVH K PL
Sbjct: 486 MSEAVGITVHMKFPL 493

[Close]

BLAST of Chy12G218220 vs. ExPASy Swiss-Prot
Match: A0A068Q609 (Phenylacetaldehyde oxime monooxygenase CYP71AN24 OS=Prunus mume OX=102107 GN=CYP71AN24 PE=1 SV=1)

HSP 1 Score: 421.4 bits (1082), Expect = 1.3e-116
Identity = 206/436 (47.25%), Postives = 295/436 (67.66%), Query Frame = 0


Query: 1   MLLKLGQTPTLVVSSPIVAREIMKTHDLKFSNRPKTTAPNLMFYSCQDVGFAPYGDYWKK 60
           M +  G+ PTL+VSS  +A+++MKT D+ F +RP+TTAP+++FY   D+ FAPYG+YW++
Sbjct: 91  MFMHFGKVPTLIVSSAEMAKDVMKTQDIVFCSRPQTTAPSILFYDGHDIAFAPYGEYWRQ 150

Query: 61  AKKMCVVELFSHKRVESFQYVRDEEIDILINKVHKGANCSES--LNLTQLLFQTSNDIVS 120
            +++CV+EL S KRV  FQY R EE+  L++K+ K +  +    +NL +LL  TSN+I+ 
Sbjct: 151 VRRICVLELLSLKRVHQFQYARVEEVAELVSKIRKASASANGAPINLGELLVSTSNNIIC 210

Query: 121 RCVLGKKFEDENGKNEFRDLYTRRVIELLSAFCVGDFFPNFEWVDVIRGLVGEMKKVSKI 180
           RC+LG+KFED+   N F +  T+ ++  + +F  GDFFP+ +W+D  RG +  +K +   
Sbjct: 211 RCILGQKFEDKE-DNWFGET-TKELMTQVMSFSFGDFFPSLKWIDRARGYLAYLKSIWLE 270

Query: 181 IDGFFDMVIEDHVIKLRSSDRCDDKKDFVDIMLQQLNDEDYTFHDHFTRDNLKGILMDMF 240
            D FFD +I++H  K    +    KKD VDI+L   ND    F    T  N+K IL DMF
Sbjct: 271 FDKFFDKLIDEH--KAAQKEGKPRKKDIVDILLDVQNDGSLDF--ELTTSNVKAILQDMF 330

Query: 241 IGGTDSTATILEWTMAELLRNPNTMKKVQQEIRTIVGKNKKKIEIKDINKMEYMKCVIKE 300
           +GG+D++ T   W M+EL +NP  MKKVQ+E+R + GK +  +E  DIN+M+Y+ CVIKE
Sbjct: 331 VGGSDTSWTAAIWLMSELSQNPRVMKKVQEEVRRVAGK-RGYVEESDINEMKYLTCVIKE 390

Query: 301 SLRLHPPVPLLVPRETTDMVDIEGYHVGAGTSVLVNVWAIQRDPKIWENPNQFIPERFME 360
           +LRLHPP PLL+PRE    V + G+ + A T V VN +A+QRDPK+W+ P++F+PERF E
Sbjct: 391 NLRLHPPAPLLLPREAMSDVKLGGFDIPAKTQVFVNAYAVQRDPKVWDKPDEFMPERFEE 450

Query: 361 ENKSIDFKGSDFELVPFGSGRRKCPGIGFGMASSECVLVNLLYWFDWNMVE--DMKGELM 420
            N  + F G DFEL+PFG+GRR CPG+ FG+AS++ VL N+LYWFDW +        E +
Sbjct: 451 NN--VGFVGQDFELIPFGAGRRVCPGLAFGVASAQYVLANMLYWFDWKLPSGGSKLAETL 510

Query: 421 DMTEENGSTVHKKIPL 433
           DM+E  G TVHKK PL
Sbjct: 511 DMSEVYGLTVHKKSPL 517

[Close]

BLAST of Chy12G218220 vs. ExPASy Swiss-Prot
Match: A0A0N9HTU1 (Desmethylyatein synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP71CU1 PE=1 SV=1)

HSP 1 Score: 401.7 bits (1031), Expect = 1.0e-110
Identity = 193/432 (44.68%), Postives = 285/432 (65.97%), Query Frame = 0


Query: 1   MLLKLGQTPTLVVSSPIVAREIMKTHDLKFSNRPKTTAPNLMFYSCQDVGFAPYGDYWKK 60
           MLL  G+TP L+VSS   A+EIMKTHDL  +NRP TTA   + Y C D+ FAPYG+YW++
Sbjct: 65  MLLHFGKTPVLIVSSQETAKEIMKTHDLALANRPLTTAARALLYDCTDISFAPYGEYWRE 124

Query: 61  AKKMCVVELFSHKRVESFQYVRDEEIDILINKVHKGANCSESLNLTQLLFQTSNDIVSRC 120
            KKM V+ L S K+++SF+ VR+E    +I ++ + +     +++T +L+  S D++ RC
Sbjct: 125 MKKMAVLNLLSIKKIQSFRSVREELASDMIKEITRLSKTGAPVDVTNMLYHFSEDLLFRC 184

Query: 121 VLGKKFEDENGKNEFRDLYTRRVIELLSAFCVGDFFPNFEWVDVIRGLVGEMKKVSKIID 180
            LG K     G+++F+ L +R  ++L+ AFC  DFFP   W+D + GL  ++KK S+ +D
Sbjct: 185 TLGFK---PKGQHKFQKL-SRDFLDLVGAFCFNDFFPGMAWMDALTGLNRKLKKGSRELD 244

Query: 181 GFFDMVIEDHVIKLRSSDRCDDKKDFVDIMLQQLNDEDYTFHDHFTRDNLKGILMDMFIG 240
            F D +IE+ +  ++      +  +F+D++L      D T     TRDN+K I++D F+G
Sbjct: 245 DFVDELIEERIAMVKDG---VEPNEFLDLLLH--THRDTTQEIKLTRDNVKAIILDTFLG 304

Query: 241 GTDSTATILEWTMAELLRNPNTMKKVQQEIRTIVGKNKKKIEIKDINKMEYMKCVIKESL 300
           G D  A+++EW MAEL+RNP+ MK  Q+E+R +VG NK K++  D+ +M ++K  +KE+L
Sbjct: 305 GIDLPASVMEWAMAELMRNPSKMKIAQEEVRKVVG-NKNKVDEDDVYQMNFLKSAVKETL 364

Query: 301 RLHPPVPLLVPRETTDMVDIEGYHVGAGTSVLVNVWAIQRDPKIWENPNQFIPERFMEEN 360
           RLHPP PLL  RE+   +++E Y +   TSV++N+W IQRDPK+W+   +FIPERFM  N
Sbjct: 365 RLHPPAPLLFARESYTSINVENYIIPPYTSVMINIWHIQRDPKLWDKAEEFIPERFM--N 424

Query: 361 KSIDFKGSDFELVPFGSGRRKCPGIGFGMASSECVLVNLLYWFDWNMVEDMKGELMDMTE 420
             ID+K  D+E +PFGSGRR CPG+ FG+A+ E  + NLLYWFDW  V D   E +DMTE
Sbjct: 425 SGIDYKSHDYEFIPFGSGRRGCPGMSFGVAAVEFAVANLLYWFDWKFVGDTTPETLDMTE 484

Query: 421 ENGSTVHKKIPL 433
           +    + KK PL
Sbjct: 485 DYCFALFKKKPL 484

[Close]

BLAST of Chy12G218220 vs. ExPASy Swiss-Prot
Match: A0A068Q6L2 (Cytochrome P450 736A117 OS=Prunus mume OX=102107 GN=CYP736A117 PE=2 SV=1)

HSP 1 Score: 382.5 bits (981), Expect = 6.5e-105
Identity = 196/436 (44.95%), Postives = 285/436 (65.37%), Query Frame = 0


Query: 1   MLLKLGQTPTLVVSSPIVAREIMKTHDLKFSNRPKTTAPNLMFYSCQDVGFAPYGDYWKK 60
           MLL  G  P LVVSS   AREI+KTHDL FS+RPK+T    + Y+ +DV  APYG+YW++
Sbjct: 84  MLLHFGSVPVLVVSSAEAAREILKTHDLTFSDRPKSTIFEKLLYNYKDVASAPYGEYWRQ 143

Query: 61  AKKMCVVELFSHKRVESFQYVRDEEIDILINKVHKGANCSESLNLTQLLFQTSNDIVSRC 120
            + +CV+ L S++RV SF+ VR+EE   +I  + KG++ S  LNL+++  + +ND+V + 
Sbjct: 144 VRSICVLNLLSNRRVRSFRSVREEETKSMIRNI-KGSS-SSVLNLSEMFVRLTNDVVCKV 203

Query: 121 VLGKKFEDENGKNE---FRDLYTRRVIELLSAFCVGDFFPNFEWVDVIRGLVGEMKKVSK 180
            LG+K+ D  G      F+++      +LL    +GD+ P   W+  + GL  ++ KV+K
Sbjct: 204 ALGRKYSDGEGGESGRMFKEI-LGEFGDLLGTVNIGDYVPWLSWLSHVNGLGAKLDKVAK 263

Query: 181 IIDGFFDMVIEDHV-IKLRSSDRCDDKKDFVDIMLQQLNDEDYTFHDHFTRDNLKGILMD 240
            +D F D V+++H+    RS D  DD+KDF+DI+L     ++ +        ++KGI++D
Sbjct: 264 QLDDFIDTVVQEHMNHSSRSGD--DDQKDFLDILL--AIQKETSAGIPIDGVSVKGIILD 323

Query: 241 MFIGGTDSTATILEWTMAELLRNPNTMKKVQQEIRTIVGKNKKKIEIKDINKMEYMKCVI 300
           MF  GTD+T + LEW M ELLR+P  M K+Q E+R IVG     I   D+ +M Y+K V 
Sbjct: 324 MFAAGTDTTYSALEWAMTELLRHPRVMNKLQNEVRGIVGNRTDVITEDDLVEMHYLKAVT 383

Query: 301 KESLRLHPPVPLLVPRETTDMVDIEGYHVGAGTSVLVNVWAIQRDPKIWENPNQFIPERF 360
           KE+LRLHPP+PLLVPR +T  V++ GY++ A T V ++ W I RDPK+++ P +F PERF
Sbjct: 384 KETLRLHPPIPLLVPRMSTRDVEVNGYNIKANTQVFISAWQIGRDPKLYDKPEEFEPERF 443

Query: 361 MEENKSIDFKGSDFELVPFGSGRRKCPGIGFGMASSECVLVNLLYWFDWNMVEDMKGELM 420
           +  N  ID+KG+DFEL+PFG+GRR CPGI F MA +E  L N+++ FDW + ++  GE +
Sbjct: 444 L--NNGIDYKGNDFELIPFGAGRRVCPGIQFAMAVNEIALANIVHKFDWALPDEASGEDL 503

Query: 421 DMTEENGSTVHKKIPL 433
           DMTE  G T HKK PL
Sbjct: 504 DMTETTGLTAHKKYPL 510

[Close]

BLAST of Chy12G218220 vs. ExPASy Swiss-Prot
Match: Q9STK8 (Cytochrome P450 71A25 OS=Arabidopsis thaliana OX=3702 GN=CYP71A25 PE=2 SV=1)

HSP 1 Score: 364.4 bits (934), Expect = 1.8e-99
Identity = 182/433 (42.03%), Postives = 284/433 (65.59%), Query Frame = 0


Query: 1   MLLKLGQTPTLVVSSPIVAREIMKTHDLKFSNRPKTTAPNLMFYSCQDVGFAPYGDYWKK 60
           MLL LG+ P L+VSS  +A+EI+KTHD  F+NRP++     + Y+ +DV  APYG+YW++
Sbjct: 65  MLLHLGRVPVLIVSSADMAQEILKTHDQAFANRPRSKLSQKLLYNNRDVASAPYGEYWRQ 124

Query: 61  AKKMCVVELFSHKRVESFQYVRDEEIDILINKVHKGANCSESLNLTQLLFQTSNDIVSRC 120
            K +CV+ L S+K V SF+ VR+EEI +++ K+ K +  S   N++++L   +ND++ R 
Sbjct: 125 MKSVCVIHLLSNKMVRSFRDVREEEITLMMAKIRKSS--SLPFNVSKVLECLTNDVICRV 184

Query: 121 VLGKKFEDENGKNEFRDLYTRRVIELLSAFCVGDFFPNFEWVDVIRGLVGEMKKVSKIID 180
            LG+K+    G+ +F+ L T R+ ELL  F +G F P   WVD IRG   ++ K+ K +D
Sbjct: 185 ALGRKY---GGETDFKKL-TDRLSELLGTFSIGSFVPWLAWVDWIRGWDAQLDKMGKDLD 244

Query: 181 GFFDMVIEDHVIKLRSSDRCDDKKDFVDIMLQQLNDEDYTFHDHFTRDNLKGILMDMFIG 240
            FF+ V++DH       DR  D  D +D +L+   ++   F     R ++K I +D+F+G
Sbjct: 245 DFFEKVVQDH----EDGDR-RDGTDLIDALLRVKREKSPGF--EIERVSIKAITLDVFVG 304

Query: 241 GTDSTATILEWTMAELLRNPNTMKKVQQEIRTIVGKNKKKIEIKDINKMEYMKCVIKESL 300
           G+D++ T+LEW M ELLR+P ++ ++Q+E+RTI  K K ++   DI  M+Y+K VIKE+L
Sbjct: 305 GSDTSFTLLEWAMTELLRHPKSLNRLQEEVRTIC-KGKSRVSEDDIQGMKYLKAVIKEAL 364

Query: 301 RLHPPVPLLVPRETTDMVDIEGYHVGAGTSVLVNVWAIQRDPKIW-ENPNQFIPERFMEE 360
           RLHPP P++ P E+T+ V +  YH+ AGT V++N WAI R+   W  +  +F PER ++ 
Sbjct: 365 RLHPPFPMMAPHESTEDVKLRDYHIPAGTQVMMNAWAIGREVATWGPDAEEFKPERHLD- 424

Query: 361 NKSIDFKGSDFELVPFGSGRRKCPGIGFGMASSECVLVNLLYWFDWNMVEDMKGELMDMT 420
             S+DF+G +FEL+PFG+GRR CP + F +  +E VL NL++ FDW + E+ K +  D+ 
Sbjct: 425 -TSVDFRGQNFELLPFGAGRRICPAVSFAVVLNEVVLANLVHGFDWKLPEESKEDKTDVA 481

Query: 421 EENGSTVHKKIPL 433
           E +G +VH++ PL
Sbjct: 485 ESSGFSVHREFPL 481

[Close]

BLAST of Chy12G218220 vs. ExPASy TrEMBL
Match: A0A5A7UJE1 (Cytochrome P450 71A1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G004070 PE=3 SV=1)

HSP 1 Score: 794.3 bits (2050), Expect = 2.6e-226
Identity = 384/434 (88.48%), Postives = 407/434 (93.78%), Query Frame = 0


Query: 1   MLLKLGQTPTLVVSSPIVAREIMKTHDLKFSNRPKTTAPNLMFYSCQDVGFAPYGDYWKK 60
           MLLKLGQTPTLVVSSP VA EIMKTHDLKFSNRPKTTA NLMFY CQDVGFAPYGDYW+K
Sbjct: 86  MLLKLGQTPTLVVSSPKVAGEIMKTHDLKFSNRPKTTATNLMFYGCQDVGFAPYGDYWRK 145

Query: 61  AKKMCVVELFSHKRVESFQYVRDEEIDILINKVHKGANCSESLNLTQLLFQTSNDIVSRC 120
           AKKMCV+ELFSHKRVESFQYVRDEE+DILINK+ K ANCSE LNLTQLLFQ SNDIVSRC
Sbjct: 146 AKKMCVLELFSHKRVESFQYVRDEEVDILINKIRKRANCSEGLNLTQLLFQISNDIVSRC 205

Query: 121 VLGKKFEDENGKNEFRDLYTRRVIELLSAFCVGDFFPNFEWVDVIRGLVGEMKKVSKIID 180
           VLGKKFEDENGK+EFRD+++RRV+ELL+AFCVGDFFPNF WVDVIRG+VGEMKKVSKI+D
Sbjct: 206 VLGKKFEDENGKSEFRDVHSRRVMELLAAFCVGDFFPNFGWVDVIRGVVGEMKKVSKIMD 265

Query: 181 GFFDMVIEDHVIKLRSSDRCDDKKDFVDIMLQQLNDEDYTFHDHFTRDNLKGILMDMFIG 240
           GFFDMVIEDH+IKL+SSDRCDDKKDFVDIMLQQLNDED  FHDHFTRDNLK IL DMF+G
Sbjct: 266 GFFDMVIEDHIIKLKSSDRCDDKKDFVDIMLQQLNDEDDMFHDHFTRDNLKAILTDMFVG 325

Query: 241 GTDSTATILEWTMAELLRNPNTMKKVQQEIRTIVGKNKKKIEIKDINKMEYMKCVIKESL 300
           GTDSTAT+LEWTMAELLRNPNTMKKVQQEIRTIVGKNK K+E KDINKMEYMKCV+KESL
Sbjct: 326 GTDSTATLLEWTMAELLRNPNTMKKVQQEIRTIVGKNKTKVETKDINKMEYMKCVMKESL 385

Query: 301 RLHPPVPLLVPRE-TTDMVDIEGYHVGAGTSVLVNVWAIQRDPKIWENPNQFIPERFMEE 360
           RLHP +PLLVPRE TTD+VDIEGYHV AGTSV VNVWAIQRDPKIWENPNQFIPERFMEE
Sbjct: 386 RLHPSLPLLVPRETTTDVVDIEGYHVAAGTSVFVNVWAIQRDPKIWENPNQFIPERFMEE 445

Query: 361 NKS-IDFKGSDFELVPFGSGRRKCPGIGFGMASSECVLVNLLYWFDWNMVEDMKGELMDM 420
           NKS IDFKGSDFELVPFGSGRRKCPGIGFG A+ ECVL NLLYWFDW MVEDMKGE +DM
Sbjct: 446 NKSIIDFKGSDFELVPFGSGRRKCPGIGFGSAAYECVLANLLYWFDWKMVEDMKGETLDM 505

Query: 421 TEENGSTVHKKIPL 433
           TEE+G TVHKK PL
Sbjct: 506 TEEHGITVHKKFPL 519

[Close]

BLAST of Chy12G218220 vs. ExPASy TrEMBL
Match: A0A1S3AXW4 (cytochrome P450 71A1-like OS=Cucumis melo OX=3656 GN=LOC103483957 PE=3 SV=1)

HSP 1 Score: 794.3 bits (2050), Expect = 2.6e-226
Identity = 384/434 (88.48%), Postives = 407/434 (93.78%), Query Frame = 0


Query: 1   MLLKLGQTPTLVVSSPIVAREIMKTHDLKFSNRPKTTAPNLMFYSCQDVGFAPYGDYWKK 60
           MLLKLGQTPTLVVSSP VA EIMKTHDLKFSNRPKTTA NLMFY CQDVGFAPYGDYW+K
Sbjct: 1   MLLKLGQTPTLVVSSPKVAGEIMKTHDLKFSNRPKTTATNLMFYGCQDVGFAPYGDYWRK 60

Query: 61  AKKMCVVELFSHKRVESFQYVRDEEIDILINKVHKGANCSESLNLTQLLFQTSNDIVSRC 120
           AKKMCV+ELFSHKRVESFQYVRDEE+DILINK+ K ANCSE LNLTQLLFQ SNDIVSRC
Sbjct: 61  AKKMCVLELFSHKRVESFQYVRDEEVDILINKIRKRANCSEGLNLTQLLFQISNDIVSRC 120

Query: 121 VLGKKFEDENGKNEFRDLYTRRVIELLSAFCVGDFFPNFEWVDVIRGLVGEMKKVSKIID 180
           VLGKKFEDENGK+EFRD+++RRV+ELL+AFCVGDFFPNF WVDVIRG+VGEMKKVSKI+D
Sbjct: 121 VLGKKFEDENGKSEFRDVHSRRVMELLAAFCVGDFFPNFGWVDVIRGVVGEMKKVSKIMD 180

Query: 181 GFFDMVIEDHVIKLRSSDRCDDKKDFVDIMLQQLNDEDYTFHDHFTRDNLKGILMDMFIG 240
           GFFDMVIEDH+IKL+SSDRCDDKKDFVDIMLQQLNDED  FHDHFTRDNLK IL DMF+G
Sbjct: 181 GFFDMVIEDHIIKLKSSDRCDDKKDFVDIMLQQLNDEDDMFHDHFTRDNLKAILTDMFVG 240

Query: 241 GTDSTATILEWTMAELLRNPNTMKKVQQEIRTIVGKNKKKIEIKDINKMEYMKCVIKESL 300
           GTDSTAT+LEWTMAELLRNPNTMKKVQQEIRTIVGKNK K+E KDINKMEYMKCV+KESL
Sbjct: 241 GTDSTATLLEWTMAELLRNPNTMKKVQQEIRTIVGKNKTKVETKDINKMEYMKCVMKESL 300

Query: 301 RLHPPVPLLVPRE-TTDMVDIEGYHVGAGTSVLVNVWAIQRDPKIWENPNQFIPERFMEE 360
           RLHP +PLLVPRE TTD+VDIEGYHV AGTSV VNVWAIQRDPKIWENPNQFIPERFMEE
Sbjct: 301 RLHPSLPLLVPRETTTDVVDIEGYHVAAGTSVFVNVWAIQRDPKIWENPNQFIPERFMEE 360

Query: 361 NKS-IDFKGSDFELVPFGSGRRKCPGIGFGMASSECVLVNLLYWFDWNMVEDMKGELMDM 420
           NKS IDFKGSDFELVPFGSGRRKCPGIGFG A+ ECVL NLLYWFDW MVEDMKGE +DM
Sbjct: 361 NKSIIDFKGSDFELVPFGSGRRKCPGIGFGSAAYECVLANLLYWFDWKMVEDMKGETLDM 420

Query: 421 TEENGSTVHKKIPL 433
           TEE+G TVHKK PL
Sbjct: 421 TEEHGITVHKKFPL 434

[Close]

BLAST of Chy12G218220 vs. ExPASy TrEMBL
Match: A0A6J1E7U9 (cytochrome P450 71A1-like OS=Cucurbita moschata OX=3662 GN=LOC111431445 PE=3 SV=1)

HSP 1 Score: 640.2 bits (1650), Expect = 6.4e-180
Identity = 310/432 (71.76%), Postives = 366/432 (84.72%), Query Frame = 0


Query: 1   MLLKLGQTPTLVVSSPIVAREIMKTHDLKFSNRPKTTAPNLMFYSCQDVGFAPYGDYWKK 60
           MLLKLGQTPTLVVSS  +ARE+M+THDLKFSNRPKTTA NL+FY CQD+GFAPYG YW++
Sbjct: 88  MLLKLGQTPTLVVSSAKMAREVMRTHDLKFSNRPKTTATNLLFYGCQDMGFAPYGAYWRQ 147

Query: 61  AKKMCVVELFSHKRVESFQYVRDEEIDILINKVHKGANCSESLNLTQLLFQTSNDIVSRC 120
           A+KMCV+ELFS KRVESFQY+RDEE+ +LI+++HK     E +NL++L  Q SN+IVSRC
Sbjct: 148 ARKMCVLELFSFKRVESFQYIRDEEVGVLISRIHKACAGGEVVNLSKLFLQISNNIVSRC 207

Query: 121 VLGKKFEDENGKNEFRDLYTRRVIELLSAFCVGDFFPNFEWVDVIRGLVGEMKKVSKIID 180
           V+GKKFEDENG N FRD+ +RRVIELLSAFCV DFFP F WVDV+RG +GE+K +S+ +D
Sbjct: 208 VMGKKFEDENGNNRFRDV-SRRVIELLSAFCVADFFPAFGWVDVVRGFIGELKTISRTMD 267

Query: 181 GFFDMVIEDHVIKLRSSDRCDDKKDFVDIMLQQLNDEDYTFHDHFTRDNLKGILMDMFIG 240
            F DMVIE+H  +LR+  R DDK+DF+DIML QLN +D   + HFTRDNLK IL DMF G
Sbjct: 268 EFLDMVIEEHKTELRTG-RPDDKRDFLDIML-QLNQDDMLGY-HFTRDNLKAILQDMFAG 327

Query: 241 GTDSTATILEWTMAELLRNPNTMKKVQQEIRTIVGKNKKKIEIKDINKMEYMKCVIKESL 300
           G+D++AT LEW MAEL+RNPNT+KKVQ+EIRTIVGK K KIE+ DI+KMEYMKCVIKESL
Sbjct: 328 GSDTSATALEWAMAELIRNPNTLKKVQEEIRTIVGK-KPKIEMNDISKMEYMKCVIKESL 387

Query: 301 RLHPPVPLLVPRETTDMVDIEGYHVGAGTSVLVNVWAIQRDPKIWENPNQFIPERFMEEN 360
           RLHPP+PLLVPRET+D+VDIEGYHV +GTSV VN WAIQRDP IWE PN+FIPERFME+N
Sbjct: 388 RLHPPIPLLVPRETSDVVDIEGYHVASGTSVFVNAWAIQRDPIIWERPNEFIPERFMEKN 447

Query: 361 KSIDFKGSDFELVPFGSGRRKCPGIGFGMASSECVLVNLLYWFDWNMVEDMKGELMDMTE 420
              DFKG DFE +PFGSGRRKCPG+ F +AS ECVL NLLYWFDW + EDM GEL+DM+E
Sbjct: 448 -CADFKGLDFEFIPFGSGRRKCPGLSFALASFECVLANLLYWFDWKLPEDMAGELLDMSE 507

Query: 421 ENGSTVHKKIPL 433
            +G TV KKIPL
Sbjct: 508 LHGITVRKKIPL 513

[Close]

BLAST of Chy12G218220 vs. ExPASy TrEMBL
Match: A0A6J1C7Y2 (cytochrome P450 71A1-like OS=Momordica charantia OX=3673 GN=LOC111009056 PE=3 SV=1)

HSP 1 Score: 545.8 bits (1405), Expect = 1.6e-151
Identity = 273/441 (61.90%), Postives = 339/441 (76.87%), Query Frame = 0


Query: 1   MLLKLGQTPTLVVSSPIVAREIMKTHDLKFSNRPKTTAPNLMFYSCQDVGFAPYGDYWKK 60
           M LKLG  PTLV+SS  +AREI+K+HD+ FSNR + TA N++ Y C DVGFAPYG+YW++
Sbjct: 1   MFLKLGHIPTLVISSSKLAREIIKSHDITFSNRLQITAANILLYGCHDVGFAPYGEYWRE 60

Query: 61  AKKMCVVELFSHKRVESFQYVRDEEIDILINKVHKGA-----NCSESLNLTQLLFQTSND 120
           A+K CV+EL S KRVESFQ+VRDEE+ +L+ +VHK       + S S+NL +LL  TSN+
Sbjct: 61  ARKFCVMELLSAKRVESFQHVRDEEVALLVKRVHKACIEDNDSSSSSVNLNRLLLSTSNN 120

Query: 121 IVSRCVLGKKFEDENGKNEFRDLYTRRVIELLSAFCVGDFFPNFEWVDVIRGLVGEMKKV 180
           IVSRCVLG+KFEDENGK+ F ++ +RRV+ L++ FCV DFFP F W+DV+RG +GE+K  
Sbjct: 121 IVSRCVLGEKFEDENGKSRFGEV-SRRVMVLMAGFCVADFFPGFRWIDVLRGFIGELKST 180

Query: 181 SKIIDGFFDMVIEDHVIK-LRSSDRCDDKKDFVDIMLQQLNDE--DYTFHDHFTRDNLKG 240
            + +D FFD VIE H  K +R+ D   D KDFVDIMLQ   DE  DY     F+RDN+K 
Sbjct: 181 FQTLDAFFDEVIEKHREKMMRTDDHQSDVKDFVDIMLQLQRDEALDYC----FSRDNIKA 240

Query: 241 ILMDMFIGGTDSTATILEWTMAELLRNPNTMKKVQQEIRTIVGKNKKKIEIKDINKMEYM 300
           IL+DM +GGT++TA  LEWTMAEL RNP  MKKVQ+EIR IVGK K KIE++D  KMEYM
Sbjct: 241 ILLDMLVGGTETTAIGLEWTMAELTRNPQAMKKVQEEIRRIVGK-KTKIEMEDTKKMEYM 300

Query: 301 KCVIKESLRLHPPVPLLVPRETTDMVDI-EGYHVGAGTSVLVNVWAIQRDPKIWENPNQF 360
            CVIKESLRLHP  PLLVPRET+ +V + E Y + + TS+ +N WAIQRDP+ WE+PN+F
Sbjct: 301 NCVIKESLRLHPLAPLLVPRETSGIVTLEESYQIPSKTSIWINAWAIQRDPETWESPNEF 360

Query: 361 IPERFMEENKSIDFKGSDFELVPFGSGRRKCPGIGFGMASSECVLVNLLYWFDWNMVEDM 420
           IPERFME+N S+DFKG DFE +PFGSGRRKCP I FG+AS E VL NLLYWFDW + E+M
Sbjct: 361 IPERFMEKN-SVDFKGQDFEFIPFGSGRRKCPAISFGLASFEYVLANLLYWFDWKLPEEM 420

Query: 421 KGELMDMTEENGSTVHKKIPL 433
            GEL D++EE+G TV KKIPL
Sbjct: 421 AGEL-DISEEHGLTVRKKIPL 433

[Close]

BLAST of Chy12G218220 vs. ExPASy TrEMBL
Match: A0A6J1C953 (cytochrome P450 71A1-like OS=Momordica charantia OX=3673 GN=LOC111008589 PE=3 SV=1)

HSP 1 Score: 536.2 bits (1380), Expect = 1.3e-148
Identity = 265/434 (61.06%), Postives = 328/434 (75.58%), Query Frame = 0


Query: 1   MLLKLGQTPTLVVSSPIVAREIMKTHDLKFSNRPKTTAPNLMFYSCQDVGFAPYGDYWKK 60
           M LKLG  PTL++SS  +ARE+MK+ D+ F NR + TA N + Y C D+ F   G+ W++
Sbjct: 105 MFLKLGHAPTLIISSAKLAREVMKSQDIIFCNRAQNTAANHLLYGCHDIAFGSCGELWRQ 164

Query: 61  AKKMCVVELFSHKRVESFQYVRDEEIDILINKVHKGANCSESLNLTQLLFQTSNDIVSRC 120
           AKK+CVVEL S KRV+SFQ+VRDEE+ ILI ++ K  + S S NL +LL  T N+I+SRC
Sbjct: 165 AKKICVVELLSAKRVDSFQHVRDEEVAILIKRIRKSCDSSSSTNLNKLLLATINNIISRC 224

Query: 121 VLGKKFEDENGKNEFRDLYTRRVIELLSAFCVGDFFPNFEWVDVIRGLVGEMKKVSKIID 180
           VLG+KFEDENG ++F  + TR+++ELL+ FCV DFFP F  +DV+RG +GE++   K +D
Sbjct: 225 VLGEKFEDENGDSKFGGV-TRKIMELLTRFCVADFFPGFGLIDVVRGFIGELETTFKTMD 284

Query: 181 GFFDMVIEDHVIKLRSSDRCDDKKDFVDIM--LQQLNDEDYTFHDHFTRDNLKGILMDMF 240
            FFD VIE H  K+  SD   D++DFVDIM  LQQ    DY     FTRDNLK IL+DMF
Sbjct: 285 AFFDKVIEKHREKMGESDDKSDEQDFVDIMLQLQQNKTSDY----RFTRDNLKAILLDMF 344

Query: 241 IGGTDSTATILEWTMAELLRNPNTMKKVQQEIRTIVGKNKKKIEIKDINKMEYMKCVIKE 300
           +GGTD+T+  LEWTMAEL++NP  MKKVQ+E+R IVGK K KIE KD  KMEYMKCVIKE
Sbjct: 345 VGGTDTTSVGLEWTMAELIKNPTIMKKVQKEVREIVGK-KPKIETKDTEKMEYMKCVIKE 404

Query: 301 SLRLHPPVPLLVPRETTDMVDIEGYHVGAGTSVLVNVWAIQRDPKIWENPNQFIPERFME 360
           SLRLHPP PLLVPRET  + ++ GY + + T+V +N WAIQRDPKIWE+PN+FIPERFM 
Sbjct: 405 SLRLHPPAPLLVPRETLKIANLGGYQIPSKTNVWINAWAIQRDPKIWESPNEFIPERFMG 464

Query: 361 ENKSIDFKGSDFELVPFGSGRRKCPGIGFGMASSECVLVNLLYWFDWNMVEDMKGELMDM 420
           +N S DFKG DFE +PFGSGRRKCPGI FG+AS E  L NLLYWFDW + E+M GEL DM
Sbjct: 465 KN-SFDFKGQDFEFLPFGSGRRKCPGISFGLASFEYALANLLYWFDWKLQEEMAGEL-DM 524

Query: 421 TEENGSTVHKKIPL 433
           +EE+G T HKKIPL
Sbjct: 525 SEEHGLTDHKKIPL 530

[Close]

BLAST of Chy12G218220 vs. NCBI nr
Match: XP_004152576.3 (cytochrome P450 71A1-like [Cucumis sativus])

HSP 1 Score: 853 bits (2204), Expect = 2.12e-310
Identity = 417/432 (96.53%), Postives = 422/432 (97.69%), Query Frame = 0


Query: 1   MLLKLGQTPTLVVSSPIVAREIMKTHDLKFSNRPKTTAPNLMFYSCQDVGFAPYGDYWKK 60
           MLLKLGQTPTLVVSSP VAREIMKTHDLKFSNRPKTTAPN+MFYSCQDVGFAPYGDYWKK
Sbjct: 87  MLLKLGQTPTLVVSSPRVAREIMKTHDLKFSNRPKTTAPNVMFYSCQDVGFAPYGDYWKK 146

Query: 61  AKKMCVVELFSHKRVESFQYVRDEEIDILINKVHKGANCSESLNLTQLLFQTSNDIVSRC 120
           AKKMCVVELFSHKRVESFQYVRDEEIDILINKVHKGANC E L+LTQLLFQTSNDIVSRC
Sbjct: 147 AKKMCVVELFSHKRVESFQYVRDEEIDILINKVHKGANCREGLDLTQLLFQTSNDIVSRC 206

Query: 121 VLGKKFEDENGKNEFRDLYTRRVIELLSAFCVGDFFPNFEWVDVIRGLVGEMKKVSKIID 180
           VLGKKFEDENGKNEFRDLYTRRVIELLSAFCVGDFFPNFEWVDVIRGLVGEMKKVSKIID
Sbjct: 207 VLGKKFEDENGKNEFRDLYTRRVIELLSAFCVGDFFPNFEWVDVIRGLVGEMKKVSKIID 266

Query: 181 GFFDMVIEDHVIKLRSSDRCDDKKDFVDIMLQQLNDEDYTFHDHFTRDNLKGILMDMFIG 240
           GFFDMVIEDHVIKLRS+DRCDDKKDFVDIMLQQLNDEDY FHDHFTRDNLKGILMDMFIG
Sbjct: 267 GFFDMVIEDHVIKLRSNDRCDDKKDFVDIMLQQLNDEDYMFHDHFTRDNLKGILMDMFIG 326

Query: 241 GTDSTATILEWTMAELLRNPNTMKKVQQEIRTIVGKNKKKIEIKDINKMEYMKCVIKESL 300
           GTDSTATILEWTMAELLRN NTMKKVQQEIRTIVGKNKKKIE  DINKMEYMKCVIKESL
Sbjct: 327 GTDSTATILEWTMAELLRNRNTMKKVQQEIRTIVGKNKKKIETMDINKMEYMKCVIKESL 386

Query: 301 RLHPPVPLLVPRETTDMVDIEGYHVGAGTSVLVNVWAIQRDPKIWENPNQFIPERFMEEN 360
           RLHPPVPLLVPRETTDMVDIEGY V AGTSVLVNVWAIQRDPKIWENPNQFIPERFMEEN
Sbjct: 387 RLHPPVPLLVPRETTDMVDIEGYRVRAGTSVLVNVWAIQRDPKIWENPNQFIPERFMEEN 446

Query: 361 KSIDFKGSDFELVPFGSGRRKCPGIGFGMASSECVLVNLLYWFDWNMVEDMKGELMDMTE 420
           KSIDFKGSDFELVPFGSGRRKCPGIGFG+A+SECVLVNLLYWFDW MVEDMKGELMDMTE
Sbjct: 447 KSIDFKGSDFELVPFGSGRRKCPGIGFGIAASECVLVNLLYWFDWKMVEDMKGELMDMTE 506

Query: 421 ENGSTVHKKIPL 432
           ENGSTVHKKIPL
Sbjct: 507 ENGSTVHKKIPL 518

[Close]

BLAST of Chy12G218220 vs. NCBI nr
Match: XP_008439061.1 (PREDICTED: cytochrome P450 71A1-like [Cucumis melo])

HSP 1 Score: 791 bits (2043), Expect = 3.05e-287
Identity = 384/434 (88.48%), Postives = 407/434 (93.78%), Query Frame = 0


Query: 1   MLLKLGQTPTLVVSSPIVAREIMKTHDLKFSNRPKTTAPNLMFYSCQDVGFAPYGDYWKK 60
           MLLKLGQTPTLVVSSP VA EIMKTHDLKFSNRPKTTA NLMFY CQDVGFAPYGDYW+K
Sbjct: 1   MLLKLGQTPTLVVSSPKVAGEIMKTHDLKFSNRPKTTATNLMFYGCQDVGFAPYGDYWRK 60

Query: 61  AKKMCVVELFSHKRVESFQYVRDEEIDILINKVHKGANCSESLNLTQLLFQTSNDIVSRC 120
           AKKMCV+ELFSHKRVESFQYVRDEE+DILINK+ K ANCSE LNLTQLLFQ SNDIVSRC
Sbjct: 61  AKKMCVLELFSHKRVESFQYVRDEEVDILINKIRKRANCSEGLNLTQLLFQISNDIVSRC 120

Query: 121 VLGKKFEDENGKNEFRDLYTRRVIELLSAFCVGDFFPNFEWVDVIRGLVGEMKKVSKIID 180
           VLGKKFEDENGK+EFRD+++RRV+ELL+AFCVGDFFPNF WVDVIRG+VGEMKKVSKI+D
Sbjct: 121 VLGKKFEDENGKSEFRDVHSRRVMELLAAFCVGDFFPNFGWVDVIRGVVGEMKKVSKIMD 180

Query: 181 GFFDMVIEDHVIKLRSSDRCDDKKDFVDIMLQQLNDEDYTFHDHFTRDNLKGILMDMFIG 240
           GFFDMVIEDH+IKL+SSDRCDDKKDFVDIMLQQLNDED  FHDHFTRDNLK IL DMF+G
Sbjct: 181 GFFDMVIEDHIIKLKSSDRCDDKKDFVDIMLQQLNDEDDMFHDHFTRDNLKAILTDMFVG 240

Query: 241 GTDSTATILEWTMAELLRNPNTMKKVQQEIRTIVGKNKKKIEIKDINKMEYMKCVIKESL 300
           GTDSTAT+LEWTMAELLRNPNTMKKVQQEIRTIVGKNK K+E KDINKMEYMKCV+KESL
Sbjct: 241 GTDSTATLLEWTMAELLRNPNTMKKVQQEIRTIVGKNKTKVETKDINKMEYMKCVMKESL 300

Query: 301 RLHPPVPLLVPRETT-DMVDIEGYHVGAGTSVLVNVWAIQRDPKIWENPNQFIPERFMEE 360
           RLHP +PLLVPRETT D+VDIEGYHV AGTSV VNVWAIQRDPKIWENPNQFIPERFMEE
Sbjct: 301 RLHPSLPLLVPRETTTDVVDIEGYHVAAGTSVFVNVWAIQRDPKIWENPNQFIPERFMEE 360

Query: 361 NKSI-DFKGSDFELVPFGSGRRKCPGIGFGMASSECVLVNLLYWFDWNMVEDMKGELMDM 420
           NKSI DFKGSDFELVPFGSGRRKCPGIGFG A+ ECVL NLLYWFDW MVEDMKGE +DM
Sbjct: 361 NKSIIDFKGSDFELVPFGSGRRKCPGIGFGSAAYECVLANLLYWFDWKMVEDMKGETLDM 420

Query: 421 TEENGSTVHKKIPL 432
           TEE+G TVHKK PL
Sbjct: 421 TEEHGITVHKKFPL 434

[Close]

BLAST of Chy12G218220 vs. NCBI nr
Match: KAA0055274.1 (cytochrome P450 71A1-like [Cucumis melo var. makuwa] >TYJ99197.1 cytochrome P450 71A1-like [Cucumis melo var. makuwa])

HSP 1 Score: 791 bits (2043), Expect = 7.30e-286
Identity = 384/434 (88.48%), Postives = 407/434 (93.78%), Query Frame = 0


Query: 1   MLLKLGQTPTLVVSSPIVAREIMKTHDLKFSNRPKTTAPNLMFYSCQDVGFAPYGDYWKK 60
           MLLKLGQTPTLVVSSP VA EIMKTHDLKFSNRPKTTA NLMFY CQDVGFAPYGDYW+K
Sbjct: 86  MLLKLGQTPTLVVSSPKVAGEIMKTHDLKFSNRPKTTATNLMFYGCQDVGFAPYGDYWRK 145

Query: 61  AKKMCVVELFSHKRVESFQYVRDEEIDILINKVHKGANCSESLNLTQLLFQTSNDIVSRC 120
           AKKMCV+ELFSHKRVESFQYVRDEE+DILINK+ K ANCSE LNLTQLLFQ SNDIVSRC
Sbjct: 146 AKKMCVLELFSHKRVESFQYVRDEEVDILINKIRKRANCSEGLNLTQLLFQISNDIVSRC 205

Query: 121 VLGKKFEDENGKNEFRDLYTRRVIELLSAFCVGDFFPNFEWVDVIRGLVGEMKKVSKIID 180
           VLGKKFEDENGK+EFRD+++RRV+ELL+AFCVGDFFPNF WVDVIRG+VGEMKKVSKI+D
Sbjct: 206 VLGKKFEDENGKSEFRDVHSRRVMELLAAFCVGDFFPNFGWVDVIRGVVGEMKKVSKIMD 265

Query: 181 GFFDMVIEDHVIKLRSSDRCDDKKDFVDIMLQQLNDEDYTFHDHFTRDNLKGILMDMFIG 240
           GFFDMVIEDH+IKL+SSDRCDDKKDFVDIMLQQLNDED  FHDHFTRDNLK IL DMF+G
Sbjct: 266 GFFDMVIEDHIIKLKSSDRCDDKKDFVDIMLQQLNDEDDMFHDHFTRDNLKAILTDMFVG 325

Query: 241 GTDSTATILEWTMAELLRNPNTMKKVQQEIRTIVGKNKKKIEIKDINKMEYMKCVIKESL 300
           GTDSTAT+LEWTMAELLRNPNTMKKVQQEIRTIVGKNK K+E KDINKMEYMKCV+KESL
Sbjct: 326 GTDSTATLLEWTMAELLRNPNTMKKVQQEIRTIVGKNKTKVETKDINKMEYMKCVMKESL 385

Query: 301 RLHPPVPLLVPRETT-DMVDIEGYHVGAGTSVLVNVWAIQRDPKIWENPNQFIPERFMEE 360
           RLHP +PLLVPRETT D+VDIEGYHV AGTSV VNVWAIQRDPKIWENPNQFIPERFMEE
Sbjct: 386 RLHPSLPLLVPRETTTDVVDIEGYHVAAGTSVFVNVWAIQRDPKIWENPNQFIPERFMEE 445

Query: 361 NKSI-DFKGSDFELVPFGSGRRKCPGIGFGMASSECVLVNLLYWFDWNMVEDMKGELMDM 420
           NKSI DFKGSDFELVPFGSGRRKCPGIGFG A+ ECVL NLLYWFDW MVEDMKGE +DM
Sbjct: 446 NKSIIDFKGSDFELVPFGSGRRKCPGIGFGSAAYECVLANLLYWFDWKMVEDMKGETLDM 505

Query: 421 TEENGSTVHKKIPL 432
           TEE+G TVHKK PL
Sbjct: 506 TEEHGITVHKKFPL 519

[Close]

BLAST of Chy12G218220 vs. NCBI nr
Match: XP_004152573.3 (cytochrome P450 71A1-like [Cucumis sativus] >KAE8652603.1 hypothetical protein Csa_013298 [Cucumis sativus])

HSP 1 Score: 786 bits (2030), Expect = 6.23e-284
Identity = 380/432 (87.96%), Postives = 404/432 (93.52%), Query Frame = 0


Query: 1   MLLKLGQTPTLVVSSPIVAREIMKTHDLKFSNRPKTTAPNLMFYSCQDVGFAPYGDYWKK 60
           MLLKLGQTPTLVVSSP +AREIMKTHDL FSNRPKTTA NL+FYSCQDVGF+PY D WKK
Sbjct: 85  MLLKLGQTPTLVVSSPNMAREIMKTHDLNFSNRPKTTASNLLFYSCQDVGFSPYSDCWKK 144

Query: 61  AKKMCVVELFSHKRVESFQYVRDEEIDILINKVHKGANCSESLNLTQLLFQTSNDIVSRC 120
           AKKMCVVELFSHKRVESF+YVRDEE+DILINK+ KGANCSE LNLTQLLFQTSNDIVSRC
Sbjct: 145 AKKMCVVELFSHKRVESFRYVRDEEVDILINKIRKGANCSEGLNLTQLLFQTSNDIVSRC 204

Query: 121 VLGKKFEDENGKNEFRDLYTRRVIELLSAFCVGDFFPNFEWVDVIRGLVGEMKKVSKIID 180
           VLGKKFEDENGKNEFRD+++RRV+ELLSAFCVGDFFPNF WVDVIRGLVGEMKKVSKI+D
Sbjct: 205 VLGKKFEDENGKNEFRDVHSRRVMELLSAFCVGDFFPNFGWVDVIRGLVGEMKKVSKIMD 264

Query: 181 GFFDMVIEDHVIKLRSSDRCDDKKDFVDIMLQQLNDEDYTFHDHFTRDNLKGILMDMFIG 240
           GFFDMVIEDH+IKL+SSDRCDDKKDFVDIMLQQLNDED+TFHDHFT DNLK ILMDMF+G
Sbjct: 265 GFFDMVIEDHIIKLKSSDRCDDKKDFVDIMLQQLNDEDHTFHDHFTIDNLKAILMDMFVG 324

Query: 241 GTDSTATILEWTMAELLRNPNTMKKVQQEIRTIVGKNKKKIEIKDINKMEYMKCVIKESL 300
           GTDSTAT+LEWTMAELLR+ NTMKKVQQEIRTIVG+NK KIE  DINKMEYMKCV+KES+
Sbjct: 325 GTDSTATLLEWTMAELLRDSNTMKKVQQEIRTIVGENKTKIEPTDINKMEYMKCVMKESM 384

Query: 301 RLHPPVPLLVPRETTDMVDIEGYHVGAGTSVLVNVWAIQRDPKIWENPNQFIPERFMEEN 360
           RLHP VPLLVPRET D VDIEGYHVGAGTSV VNVWAIQRDPKIWENPNQFIPERFMEEN
Sbjct: 385 RLHPSVPLLVPRETIDKVDIEGYHVGAGTSVFVNVWAIQRDPKIWENPNQFIPERFMEEN 444

Query: 361 KSIDFKGSDFELVPFGSGRRKCPGIGFGMASSECVLVNLLYWFDWNMVEDMKGELMDMTE 420
           KSIDFKGS+FELVPFGSGRRKCPGI FG A+ ECVL NLLYWFDW MVE  K E +DMTE
Sbjct: 445 KSIDFKGSNFELVPFGSGRRKCPGIEFGSAAYECVLANLLYWFDWKMVEGRKEETLDMTE 504

Query: 421 ENGSTVHKKIPL 432
           E+G TVHKKIPL
Sbjct: 505 EHGITVHKKIPL 516

[Close]

BLAST of Chy12G218220 vs. NCBI nr
Match: XP_038895708.1 (cytochrome P450 71A1-like [Benincasa hispida])

HSP 1 Score: 673 bits (1737), Expect = 2.59e-239
Identity = 328/442 (74.21%), Postives = 379/442 (85.75%), Query Frame = 0


Query: 1   MLLKLGQTPTLVVSSPIVAREIMKTHDLKFSNRPKTTAPNLMFYSCQDVGFAPYGDYWKK 60
           MLLKLGQTPTLVVSS  +ARE+MKTHDL FSNRPKT A NL+FY CQDVGFAPYG+ W++
Sbjct: 84  MLLKLGQTPTLVVSSAKMAREVMKTHDLIFSNRPKTRATNLLFYGCQDVGFAPYGENWRQ 143

Query: 61  AKKMCVVELFSHKRVESFQYVRDEEIDILINKVHKGANCS-----ESLNLTQLLFQTSND 120
           AKKMCV+ELFS KRVESFQY+RDEEID LIN++HK ANCS     E LNL+QL  Q SN+
Sbjct: 144 AKKMCVLELFSLKRVESFQYIRDEEIDTLINRIHKAANCSVGGGGEGLNLSQLFSQISNN 203

Query: 121 IVSRCVLGKKFEDENGKNEFRDLYTRRVIELLSAFCVGDFFPNFEWVDVIRGLVGEMKKV 180
           IVSRCV+GKKFEDENGK+++ ++ +RR IELLS FCVGDFFP+F WVDVIRG+VGEMK V
Sbjct: 204 IVSRCVMGKKFEDENGKSKYGEV-SRRTIELLSVFCVGDFFPSFGWVDVIRGVVGEMKLV 263

Query: 181 SKIIDGFFDMVIEDHVIKLRSSDRCDDKKDFVDIMLQQLNDEDYTFHDHFTRDNLKGILM 240
           SK +D F+DMVIE+H+ K RS +  D K DFVDIMLQ LN++D     HFTRDNLKGI+ 
Sbjct: 264 SKKMDEFYDMVIEEHITKSRSDEANDHKMDFVDIMLQ-LNNQDDMLDYHFTRDNLKGIIQ 323

Query: 241 DMFIGGTDSTATILEWTMAELLRNPNTMKKVQQEIRTIVGKNKKKIEIKDINKMEYMKCV 300
           DMF GG+D+TAT LEWTMAEL+R+PNTMKKVQ+EIRTIVGKNK KIE  DI KMEYMKCV
Sbjct: 324 DMFAGGSDTTATTLEWTMAELIRSPNTMKKVQEEIRTIVGKNKAKIETNDIRKMEYMKCV 383

Query: 301 IKESLRLHPPVPLLVPRETTDMVDIEGYHVGAGTSVLVNVWAIQRDPKIWENPNQFIPER 360
           IKESLRLHPPVPLLVPRET DMVDIEGYHV +GTSV VNVWAIQRDP+IWE+PN+FIPER
Sbjct: 384 IKESLRLHPPVPLLVPRETLDMVDIEGYHVESGTSVFVNVWAIQRDPEIWESPNEFIPER 443

Query: 361 FMEENKSIDFKGSDFELVPFGSGRRKCPGIGFGMASSECVLVNLLYWFDWNMVEDMKGEL 420
           FM E KS+DFKG DFEL+PFGSGRRKCPG+ FG+AS ECVL +LLYWFDW + +DMKGE+
Sbjct: 444 FMNEKKSVDFKGLDFELIPFGSGRRKCPGLAFGLASLECVLASLLYWFDWKLPQDMKGEV 503

Query: 421 MDMTEENGSTVHKKIPLS-HPI 436
           +DM+E +G T+HKK PL  HP+
Sbjct: 504 LDMSEVHGITIHKKFPLFLHPL 523

[Close]

BLAST of Chy12G218220 vs. TAIR 10
Match: AT3G48280.1 (cytochrome P450, family 71, subfamily A, polypeptide 25 )

HSP 1 Score: 364.4 bits (934), Expect = 1.3e-100
Identity = 182/433 (42.03%), Postives = 284/433 (65.59%), Query Frame = 0


Query: 1   MLLKLGQTPTLVVSSPIVAREIMKTHDLKFSNRPKTTAPNLMFYSCQDVGFAPYGDYWKK 60
           MLL LG+ P L+VSS  +A+EI+KTHD  F+NRP++     + Y+ +DV  APYG+YW++
Sbjct: 65  MLLHLGRVPVLIVSSADMAQEILKTHDQAFANRPRSKLSQKLLYNNRDVASAPYGEYWRQ 124

Query: 61  AKKMCVVELFSHKRVESFQYVRDEEIDILINKVHKGANCSESLNLTQLLFQTSNDIVSRC 120
            K +CV+ L S+K V SF+ VR+EEI +++ K+ K +  S   N++++L   +ND++ R 
Sbjct: 125 MKSVCVIHLLSNKMVRSFRDVREEEITLMMAKIRKSS--SLPFNVSKVLECLTNDVICRV 184

Query: 121 VLGKKFEDENGKNEFRDLYTRRVIELLSAFCVGDFFPNFEWVDVIRGLVGEMKKVSKIID 180
            LG+K+    G+ +F+ L T R+ ELL  F +G F P   WVD IRG   ++ K+ K +D
Sbjct: 185 ALGRKY---GGETDFKKL-TDRLSELLGTFSIGSFVPWLAWVDWIRGWDAQLDKMGKDLD 244

Query: 181 GFFDMVIEDHVIKLRSSDRCDDKKDFVDIMLQQLNDEDYTFHDHFTRDNLKGILMDMFIG 240
            FF+ V++DH       DR  D  D +D +L+   ++   F     R ++K I +D+F+G
Sbjct: 245 DFFEKVVQDH----EDGDR-RDGTDLIDALLRVKREKSPGF--EIERVSIKAITLDVFVG 304

Query: 241 GTDSTATILEWTMAELLRNPNTMKKVQQEIRTIVGKNKKKIEIKDINKMEYMKCVIKESL 300
           G+D++ T+LEW M ELLR+P ++ ++Q+E+RTI  K K ++   DI  M+Y+K VIKE+L
Sbjct: 305 GSDTSFTLLEWAMTELLRHPKSLNRLQEEVRTIC-KGKSRVSEDDIQGMKYLKAVIKEAL 364

Query: 301 RLHPPVPLLVPRETTDMVDIEGYHVGAGTSVLVNVWAIQRDPKIW-ENPNQFIPERFMEE 360
           RLHPP P++ P E+T+ V +  YH+ AGT V++N WAI R+   W  +  +F PER ++ 
Sbjct: 365 RLHPPFPMMAPHESTEDVKLRDYHIPAGTQVMMNAWAIGREVATWGPDAEEFKPERHLD- 424

Query: 361 NKSIDFKGSDFELVPFGSGRRKCPGIGFGMASSECVLVNLLYWFDWNMVEDMKGELMDMT 420
             S+DF+G +FEL+PFG+GRR CP + F +  +E VL NL++ FDW + E+ K +  D+ 
Sbjct: 425 -TSVDFRGQNFELLPFGAGRRICPAVSFAVVLNEVVLANLVHGFDWKLPEESKEDKTDVA 481

Query: 421 EENGSTVHKKIPL 433
           E +G +VH++ PL
Sbjct: 485 ESSGFSVHREFPL 481

[Close]

BLAST of Chy12G218220 vs. TAIR 10
Match: AT2G30750.1 (cytochrome P450, family 71, subfamily A, polypeptide 12 )

HSP 1 Score: 362.5 bits (929), Expect = 4.9e-100
Identity = 177/433 (40.88%), Postives = 277/433 (63.97%), Query Frame = 0


Query: 1   MLLKLGQTPTLVVSSPIVAREIMKTHDLKFSNRPKTTAPNLMFYSCQDVGFAPYGDYWKK 60
           MLL  G+ P LVVSS   A+E++KTHDLKF+NRP++ A + +    +DV F PYG+YW++
Sbjct: 74  MLLHFGRVPILVVSSGEAAQEVLKTHDLKFANRPRSKAVHGLMNGGRDVVFGPYGEYWRQ 133

Query: 61  AKKMCVVELFSHKRVESFQYVRDEEIDILINKVHKGANCSESLNLTQLLFQTSNDIVSRC 120
            K +C++ L ++K V SF+ +R+EE++ +I K+ K ++ S S NL++L     +D+ SR 
Sbjct: 134 MKSVCILNLLTNKMVASFEKIREEELNEMIKKLEKASSSSSSENLSELFVTLPSDVTSRI 193

Query: 121 VLGKKFEDENGKNEFRDLYTRRVIELLSAFCVGDFFPNFEWVDVIRGLVGEMKKVSKIID 180
            LG+K  ++    + +    R+++ELL  F +GD+ P   W+D I G    +K+VS+   
Sbjct: 194 ALGRKHSEDETARDLKK-RVRQIMELLGEFPIGDYVPALAWIDRINGFNARIKEVSQGFS 253

Query: 181 GFFDMVIEDHVIKLRSSDRCDDKKDFVDIMLQQLNDEDYTFHDHFTRDNLKGILMDMFIG 240
              D V+++H+      +  + K+DFVDI+L   +++   F     RD++K +++DMFIG
Sbjct: 254 DLMDKVVQEHL------EAGNHKEDFVDILLSIESEKSIGFQAQ--RDDIKFMILDMFIG 313

Query: 241 GTDSTATILEWTMAELLRNPNTMKKVQQEIRTIVGKNKKKIEIKDINKMEYMKCVIKESL 300
           GT +++T+LEW M EL+RNPN MKK+Q EIR+ +  +   I+ KD+  M+Y+K VIKE  
Sbjct: 314 GTSTSSTLLEWIMTELIRNPNVMKKLQDEIRSTIRPHGSYIKEKDVENMKYLKAVIKEVF 373

Query: 301 RLHPPVPLLVPRETTDMVDIEGYHVGAGTSVLVNVWAIQRDPKIW-ENPNQFIPERFMEE 360
           R+HPP+PL++PR  ++ V ++GY++ AGT V++N WAIQRDP IW  +  +F PER ++ 
Sbjct: 374 RVHPPLPLILPRLLSEDVKVKGYNIAAGTEVIINAWAIQRDPAIWGPDAEEFKPERHLD- 433

Query: 361 NKSIDFKGSDFELVPFGSGRRKCPGIGFGMASSECVLVNLLYWFDWNMVEDMKGELMDMT 420
             ++D+ G D   +PFGSGRR CPGI   +   E  + NL+  FDW       G+  D+T
Sbjct: 434 -STLDYHGKDLNFIPFGSGRRICPGINLALGLVEVTVANLVGRFDWRAEAGPNGDQPDLT 493

Query: 421 EENGSTVHKKIPL 433
           E  G  V +K PL
Sbjct: 494 EAFGLDVCRKFPL 495

[Close]

BLAST of Chy12G218220 vs. TAIR 10
Match: AT3G48270.1 (cytochrome P450, family 71, subfamily A, polypeptide 26 )

HSP 1 Score: 362.1 bits (928), Expect = 6.4e-100
Identity = 180/433 (41.57%), Postives = 282/433 (65.13%), Query Frame = 0


Query: 1   MLLKLGQTPTLVVSSPIVAREIMKTHDLKFSNRPKTTAPNLMFYSCQDVGFAPYGDYWKK 60
           MLL  G+ P LVVSS  +AR+++KTHD  F++RP++     + Y   DV  APYG+YW++
Sbjct: 66  MLLHFGRVPVLVVSSAELARDVLKTHDRVFASRPRSKIFEKLLYDKHDVASAPYGEYWRQ 125

Query: 61  AKKMCVVELFSHKRVESFQYVRDEEIDILINKVHKGANCSESLNLTQLLFQTSNDIVSRC 120
            K +CV+ LFS+K V SF+ VR+EEI +++ K+ K  + S  +NL+++L   +ND++ + 
Sbjct: 126 MKSVCVLHLFSNKMVRSFREVREEEISLMMEKIRK--SISLPVNLSKILVSLTNDVICKV 185

Query: 121 VLGKKFEDENGKNEFRDLYTRRVIELLSAFCVGDFFPNFEWVDVIRGLVGEMKKVSKIID 180
            LG+K+    G+ +F++L   R+ +LL  F VG + P   W+D IRGL  +++K +  +D
Sbjct: 186 ALGRKY---GGETDFKEL-MERLNKLLGTFSVGSYVPWLAWIDWIRGLDCQLEKTANDVD 245

Query: 181 GFFDMVIEDHVIKLRSSDRCDDKKDFVDIMLQQLNDEDYTFHDHFTRDNLKGILMDMFIG 240
            FF+ V++DHV      D   D  DFVD++L    D+   F     R ++K I+M++F+G
Sbjct: 246 KFFERVVQDHV------DGNRDMTDFVDVLLAIQRDKTVGF--EINRVSIKAIVMNVFVG 305

Query: 241 GTDSTATILEWTMAELLRNPNTMKKVQQEIRTIVGKNKKKIEIKDINKMEYMKCVIKESL 300
           GTD+++T++EW M ELLR+P  +K++Q+E+RTI  K+K  +  ++I  M Y+K VIKE+L
Sbjct: 306 GTDTSSTLMEWAMTELLRHPKCLKRLQEEVRTIC-KDKSSVSEEEIQNMSYLKAVIKEAL 365

Query: 301 RLHPPVPLLVPRETTDMVDIEGYHVGAGTSVLVNVWAIQRDPKIW-ENPNQFIPERFMEE 360
           RLHPP+PL+VP E+T  V +  +H+ AGT VL+N WAI R+   W  +  +F PER ++ 
Sbjct: 366 RLHPPLPLMVPHESTQDVRLGDHHIPAGTQVLINAWAIGREAATWGPDVEEFRPERHLD- 425

Query: 361 NKSIDFKGSDFELVPFGSGRRKCPGIGFGMASSECVLVNLLYWFDWNMVEDMKGELMDMT 420
             S+D++G  FEL+PFGSGRR CP I F +  +E VL NL++ FDW +  +   +  ++ 
Sbjct: 426 -SSVDYRGQAFELIPFGSGRRICPAISFAVVLNEVVLANLVHRFDWRLSVESTEDQTEVA 481

Query: 421 EENGSTVHKKIPL 433
           E  G  +H+  PL
Sbjct: 486 ESTGIAIHRMFPL 481

[Close]

BLAST of Chy12G218220 vs. TAIR 10
Match: AT2G30770.1 (cytochrome P450, family 71, subfamily A, polypeptide 13 )

HSP 1 Score: 360.5 bits (924), Expect = 1.9e-99
Identity = 176/433 (40.65%), Postives = 281/433 (64.90%), Query Frame = 0


Query: 1   MLLKLGQTPTLVVSSPIVAREIMKTHDLKFSNRPKTTAPNLMFYSCQDVGFAPYGDYWKK 60
           MLL  G+ P LVVSS   A+E++KTHD KF+NRP++ A + +    +DV FAPYG+YW++
Sbjct: 74  MLLHFGRVPILVVSSGEAAQEVLKTHDHKFANRPRSKAVHGLMNGGRDVVFAPYGEYWRQ 133

Query: 61  AKKMCVVELFSHKRVESFQYVRDEEIDILINKVHKGANCSESLNLTQLLFQTSNDIVSRC 120
            K +C++ L ++K VESF+ VR++E++ +I K+ K ++ S S NL++L     +D+ SR 
Sbjct: 134 MKSVCILNLLTNKMVESFEKVREDEVNAMIEKLEKASSSSSSENLSELFITLPSDVTSRV 193

Query: 121 VLGKKFEDENGKNEFRDLYTRRVIELLSAFCVGDFFPNFEWVDVIRGLVGEMKKVSKIID 180
            LG+K  ++    + +    R+++ELL  F +G++ P   W+D IRG   ++K+VS+   
Sbjct: 194 ALGRKHSEDETARDLKK-RVRQIMELLGEFPIGEYVPILAWIDGIRGFNNKIKEVSRGFS 253

Query: 181 GFFDMVIEDHVIKLRSSDRCDDKKDFVDIMLQQLNDEDYTFHDHFTRDNLKGILMDMFIG 240
              D V+++H+      +  +DK DFVDI+L    D++  F     R+++K +++DMFIG
Sbjct: 254 DLMDKVVQEHL------EASNDKADFVDILLSIEKDKNSGF--QVQRNDIKFMILDMFIG 313

Query: 241 GTDSTATILEWTMAELLRNPNTMKKVQQEIRTIVGKNKKKIEIKDINKMEYMKCVIKESL 300
           GT +T+T+LEWTM EL+R+P +MKK+Q EIR+ +  +   I+ K++  M+Y+K VIKE L
Sbjct: 314 GTSTTSTLLEWTMTELIRSPKSMKKLQDEIRSTIRPHGSYIKEKEVENMKYLKAVIKEVL 373

Query: 301 RLHPPVPLLVPRETTDMVDIEGYHVGAGTSVLVNVWAIQRDPKIW-ENPNQFIPERFMEE 360
           RLHP +P+++PR  ++ V ++GY++ AGT V++N WAIQRD  IW  +  +F PER ++ 
Sbjct: 374 RLHPSLPMILPRLLSEDVKVKGYNIAAGTEVIINAWAIQRDTAIWGPDAEEFKPERHLD- 433

Query: 361 NKSIDFKGSDFELVPFGSGRRKCPGIGFGMASSECVLVNLLYWFDWNMVEDMKGELMDMT 420
              +D+ G +   +PFGSGRR CPGI   +  +E  + NL+  FDW +     G+  D+T
Sbjct: 434 -SGLDYHGKNLNYIPFGSGRRICPGINLALGLAEVTVANLVGRFDWRVEAGPNGDQPDLT 493

Query: 421 EENGSTVHKKIPL 433
           E  G  V +K PL
Sbjct: 494 EAIGIDVCRKFPL 495

[Close]

BLAST of Chy12G218220 vs. TAIR 10
Match: AT3G26210.1 (cytochrome P450, family 71, subfamily B, polypeptide 23 )

HSP 1 Score: 360.5 bits (924), Expect = 1.9e-99
Identity = 176/434 (40.55%), Postives = 275/434 (63.36%), Query Frame = 0


Query: 1   MLLKLGQTPTLVVSSPIVAREIMKTHDLKFSNRPKTTAPNLMFYSCQDVGFAPYGDYWKK 60
           M L+LG  P +V+SS   A E++KTHDL   +RP+T A   + Y+ +D+GFAPYG+ W+ 
Sbjct: 66  MQLQLGYVPLVVISSNQAAEEVLKTHDLDCCSRPETIASKTISYNFKDIGFAPYGEEWRA 125

Query: 61  AKKMCVVELFSHKRVESFQYVRDEEIDILINKVHKGANCSESLNLTQLLFQTSNDIVSRC 120
            +K+ V+ELFS K+  SF+Y+R+EE D+L+ K+ + +     +NL + LF  S  IV R 
Sbjct: 126 LRKLAVIELFSLKKFNSFRYIREEENDLLVKKLSEASEKQSPVNLKKALFTLSASIVCRL 185

Query: 121 VLGKKFEDEN--GKNEFRDLYTRRVIELLSAFCVGDFFPNFEWVDVIRGLVGEMKKVSKI 180
             G+   +     ++   DL +R   ++ + F   +FFP    +D I G    + ++   
Sbjct: 186 AFGQNLHESEFIDEDSMEDLASRSE-KIQAKFAFSNFFPGGWILDKITGQSKSLNEIFAD 245

Query: 181 IDGFFDMVIEDHVIKLRSSDRCDDKKDFVDIMLQQLNDEDYTFHDHFTRDNLKGILMDMF 240
           +DGFF+ V++DH   L+   +  +  D VD+M+  +N +        T D++KGI+ D+F
Sbjct: 246 LDGFFNQVLDDH---LKPGRKVLETPDVVDVMIDMMNKQSQDGSFKLTTDHIKGIISDIF 305

Query: 241 IGGTDSTATILEWTMAELLRNPNTMKKVQQEIRTIVGKNKKKIEIKDINKMEYMKCVIKE 300
           + G +++AT + W M EL+RNP  MKKVQ E+RT++G+ + +I  +D+N++ Y K VIKE
Sbjct: 306 LAGVNTSATTILWAMTELIRNPRVMKKVQDEVRTVLGEKRDRITEQDLNQLNYFKLVIKE 365

Query: 301 SLRLHPPVPLLVPRETTDMVDIEGYHVGAGTSVLVNVWAIQRDPKIWENPNQFIPERFME 360
           + RLHP  PLL+PRE    + I+GY +   T ++VNV+AI RDP +WENP +F PERF++
Sbjct: 366 TFRLHPAAPLLLPREAMAKIKIQGYDIPEKTQIMVNVYAIGRDPDLWENPEEFKPERFVD 425

Query: 361 ENKSIDFKGSDFELVPFGSGRRKCPGIGFGMASSECVLVNLLYWFDWNMVEDMKGELMDM 420
              S+D++G +FEL+PFGSGRR CPG+  G+A+ E  L+NLLY+FDW + E    + +D+
Sbjct: 426 --SSVDYRGLNFELLPFGSGRRICPGMTMGIATVELGLLNLLYFFDWGLPEGRTVKDIDL 485

Query: 421 TEENGSTVHKKIPL 433
            EE    + KK+ L
Sbjct: 486 EEEGAIIIGKKVSL 493

The following BLAST results are available for this feature:

BLAST of Chy12G218220 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P24465	5.6e-125	49.66	Cytochrome P450 71A1 OS=Persea americana OX=3435 GN=CYP71A1 PE=1 SV=2	[more]
A0A068Q609	1.3e-116	47.25	Phenylacetaldehyde oxime monooxygenase CYP71AN24 OS=Prunus mume OX=102107 GN=CYP...	[more]
A0A0N9HTU1	1.0e-110	44.68	Desmethylyatein synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP71CU1 PE=1 ...	[more]
A0A068Q6L2	6.5e-105	44.95	Cytochrome P450 736A117 OS=Prunus mume OX=102107 GN=CYP736A117 PE=2 SV=1	[more]
Q9STK8	1.8e-99	42.03	Cytochrome P450 71A25 OS=Arabidopsis thaliana OX=3702 GN=CYP71A25 PE=2 SV=1	[more]

BLAST of Chy12G218220 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7UJE1	2.6e-226	88.48	Cytochrome P450 71A1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]
A0A1S3AXW4	2.6e-226	88.48	cytochrome P450 71A1-like OS=Cucumis melo OX=3656 GN=LOC103483957 PE=3 SV=1	[more]
A0A6J1E7U9	6.4e-180	71.76	cytochrome P450 71A1-like OS=Cucurbita moschata OX=3662 GN=LOC111431445 PE=3 SV=...	[more]
A0A6J1C7Y2	1.6e-151	61.90	cytochrome P450 71A1-like OS=Momordica charantia OX=3673 GN=LOC111009056 PE=3 SV...	[more]
A0A6J1C953	1.3e-148	61.06	cytochrome P450 71A1-like OS=Momordica charantia OX=3673 GN=LOC111008589 PE=3 SV...	[more]

BLAST of Chy12G218220 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004152576.3	2.12e-310	96.53	cytochrome P450 71A1-like [Cucumis sativus]	[more]
XP_008439061.1	3.05e-287	88.48	PREDICTED: cytochrome P450 71A1-like [Cucumis melo]	[more]
KAA0055274.1	7.30e-286	88.48	cytochrome P450 71A1-like [Cucumis melo var. makuwa] >TYJ99197.1 cytochrome P450...	[more]
XP_004152573.3	6.23e-284	87.96	cytochrome P450 71A1-like [Cucumis sativus] >KAE8652603.1 hypothetical protein C...	[more]
XP_038895708.1	2.59e-239	74.21	cytochrome P450 71A1-like [Benincasa hispida]	[more]

BLAST of Chy12G218220 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G48280.1	1.3e-100	42.03	cytochrome P450, family 71, subfamily A, polypeptide 25	[more]
AT2G30750.1	4.9e-100	40.88	cytochrome P450, family 71, subfamily A, polypeptide 12	[more]
AT3G48270.1	6.4e-100	41.57	cytochrome P450, family 71, subfamily A, polypeptide 26	[more]
AT2G30770.1	1.9e-99	40.65	cytochrome P450, family 71, subfamily A, polypeptide 13	[more]
AT3G26210.1	1.9e-99	40.55	cytochrome P450, family 71, subfamily B, polypeptide 23	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 240..257 score: 33.51 coord: 294..305 score: 45.15 coord: 373..382 score: 56.32
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 3..423 e-value: 4.3E-92 score: 309.3
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 372..382 score: 53.04 coord: 229..246 score: 31.91 coord: 112..130 score: 16.81 coord: 293..311 score: 38.15 coord: 249..275 score: 35.02 coord: 18..39 score: 23.22 coord: 334..358 score: 39.02 coord: 382..405 score: 25.46
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 1..437 e-value: 4.0E-112 score: 377.2
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 2..432
None	No IPR available	PANTHER	PTHR47956:SF10	CYTOCHROME P450 FAMILY 71 PROTEIN	coord: 1..433
None	No IPR available	PANTHER	PTHR47956	CYTOCHROME P450 71B11-RELATED	coord: 1..433
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 375..384

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy12G218220.1	Chy12G218220.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen