Chy12G216700 (gene) Cucumber (hystrix) v1

Overview


Name	Chy12G216700
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	Lactoylglutathione lyase
Location	chrH12: 15824833 .. 15827236 (-)
RNA-Seq Expression	Chy12G216700
Synteny	Chy12G216700

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACAGAGCTGAATAAACATGTGGCTCCTGATGGGGATGAACAATTGTTGGAATGGCCCGGGAAAGATAATCGGCGCTTTGTTCGTGCAGTTTATCGTGTCGGTGATCTCGATCGTACCATCAAGTATATATTCATCAATCTTGCTTCAAATCTCTGTGTCTTCTATCCTTCTTTATGGTAATGCATTTTCTATCTGCAGATTCTACACTGAATGCTTAGGAATGAAACTCCTAAGAAAATATGAAGTCCAAAATGAGAAACACACCAAAGCATTGATGGGGTTTGGCCCTGAGGAATCTTCGTTCGTACTTGAGTTGACTTATGGTACGTGGTCTTAATAAAAGTAATACTTACATGCTAGCTAGATTACTGTCATTCTTTACACATAGTACCACGTTACAAATTTTTGTAAAAAATCGTTGTAATATCGGTTAGATGATATAACATTAAATTTACCTTTACCTATTAACTTGAAATTTTAAGTCAATTAGTGACTTAAAGATAGTATCAAAGTAGATGATCTAAAAGTCATGTATTCAAATCCCTAAAATGTTATTTCTATTTTCACCTATTGTATCTTTTATATATTTGAAAGGATTATTAGATTAATTTTACCTTCACTCATTCGACTAAAAGATAAGTACTGTGTCCGAAAGAAGTCATAATCTCCTATGTTGTGTTAAAAATATGCAGAACATGGGGTAACTTCATATGATATTGGCACTGGATTTGGACATTTTGCCATTGCTACTCAGGATGTGGGTGAAGTCAATTTCTTCATGGTTTTTATTTACCTGAAAATTGACATGAACGATATTAACATGAATTGTTTTTTTGTGATTTTCCAAGGTCTACAAAATGGTTGAAAATGTAAGGGCCAAGGGTGGTGAGAATATGATCATTAGAGAACCTTTTGAGCTGAAAGGCAGCCCTAATTTTTTGCTGGCCTACGTGAAAGATCCTAATGGTTATATCTTTGAGCTCATTCAAAGAGCCCAAACTGAGCCCCTCTGTCATTTAATGCTTCGTGTTGCTGATTTACAACGCTCTATTGACTTCTACCAAAAGGTAAAATCACATATTTTCAAAAAGCTTCAAAAAATTTAGCTCTTAAACTTTAGTATAAAAGAATTTAGTCACTTTTAACAATTTAATCAGTGTTACTTTTTATTGTGTAACAATTTTGATCTTTATAGTTTCTAACTAAGTTGTTTTCGTGTAAGTTAAGAGACTAAAAGTGATTTTTAAAAAAAAATTGATAAAAAAGTTAGGATGTTGTTAGAAGTTTGTTAAATTTCAATGATGATGGGAGTTTCTCATGATGACCAAAGAAAACATAAAATAGAATTAAGCACGGATTATGGCAGACGATAGAAAAATACATCAGAAACGTTTTTACTATCATACAGAGAGATATCTTTCTGCTGTGCTACTAACATCTTATTTAACATTCATTTGGTTTCTCATTTTTTGATTTTTGAAAAATAAGCTCATAGACACTATTTTACTTATTGATTTCTTCATTTTCTTACCAACTTTTGATGAGTGTTAAAAAACGAAGTGAAATCTTAAAAACTAAAACTGATAGTCTTTACAAACTTGTTTTTGTTATTCAAATTTAGTTGAGAATGAAAAAGATAAAAATTGTGTGAAAAAAGTCATTAAGTAAAAAACGACAATAATAAAAGAAACTAAATGGTTATGGAACCAAACCCACGAAATTTTCTATCCTAAATAAGAATTTGTTTCCACATACAGGCATTGGGAATGAGGGTACTTACCAAGGTTGAGAGTGTTGAACAGAAGGTGATGAACTTTAAACTTTTACTTTCATCAATCTTAGCAAATTGAAAGTTGACAAAATGCTATAATTTTCAATGCACAAACCAATTGACTCTGCAGTACGCCATAGCCCTAATGGGATATGCAGATGAGTTAGAGACAACTGCTCTGGAGTTGAATTACAATCATGGTGTTACACAACATTCCAAGGGAAATGGATATTCCCAGGTCAAAATTTTCCTTTTAACTTGGAGTTTGTCCTTGATTAAGAACCACTAATTCCCATACTTGTGCTAAATTTTTGTTTTGATTTCAGGTTGCTATTGGCACCGATGATGTATACAAGAGTGCTGAGATTGTTAATCTCATTACTAAAGAACTTGGTGGAAAGATAATACAACCACCCAGTTTGGATAGCCAAATGAACTCCAAGATTACCAGTTTTCTTGATCCTGATGGCTGGAAAATTGTGAGTTTTCGAATTTTGTTTGAAATTCTAGAGGATTGCTTTCATATAATTTTGTTTTCTTGTTTTGTTATGTTTAAGGAATGCTTAAAACCAAGATTTTCTCTTTTAGGTTCTTGTAGACAATGAAGATTATCTGAAGGGAATGCAGTGA

mRNA sequence

ATGACAGAGCTGAATAAACATGTGGCTCCTGATGGGGATGAACAATTGTTGGAATGGCCCGGGAAAGATAATCGGCGCTTTGTTCGTGCAGTTTATCGTGTCGGTGATCTCGATCGTACCATCAAATTCTACACTGAATGCTTAGGAATGAAACTCCTAAGAAAATATGAAGTCCAAAATGAGAAACACACCAAAGCATTGATGGGGTTTGGCCCTGAGGAATCTTCGTTCGTACTTGAGTTGACTTATGAACATGGGGTAACTTCATATGATATTGGCACTGGATTTGGACATTTTGCCATTGCTACTCAGGATGTGGGTGAAGTCAATTTCTTCATGGTCTACAAAATGGTTGAAAATGTAAGGGCCAAGGGTGGTGAGAATATGATCATTAGAGAACCTTTTGAGCTGAAAGGCAGCCCTAATTTTTTGCTGGCCTACGTGAAAGATCCTAATGGTTATATCTTTGAGCTCATTCAAAGAGCCCAAACTGAGCCCCTCTGTCATTTAATGCTTCGTGTTGCTGATTTACAACGCTCTATTGACTTCTACCAAAAGGCATTGGGAATGAGGGTACTTACCAAGGTTGAGAGTGTTGAACAGAAGTACGCCATAGCCCTAATGGGATATGCAGATGAGTTAGAGACAACTGCTCTGGAGTTGAATTACAATCATGGTGTTACACAACATTCCAAGGGAAATGGATATTCCCAGGTTGCTATTGGCACCGATGATGTATACAAGAGTGCTGAGATTGTTAATCTCATTACTAAAGAACTTGGTGGAAAGATAATACAACCACCCAGTTTGGATAGCCAAATGAACTCCAAGATTACCAGTTTTCTTGATCCTGATGGCTGGAAAATTGTTCTTGTAGACAATGAAGATTATCTGAAGGGAATGCAGTGA

Coding sequence (CDS)

ATGACAGAGCTGAATAAACATGTGGCTCCTGATGGGGATGAACAATTGTTGGAATGGCCCGGGAAAGATAATCGGCGCTTTGTTCGTGCAGTTTATCGTGTCGGTGATCTCGATCGTACCATCAAATTCTACACTGAATGCTTAGGAATGAAACTCCTAAGAAAATATGAAGTCCAAAATGAGAAACACACCAAAGCATTGATGGGGTTTGGCCCTGAGGAATCTTCGTTCGTACTTGAGTTGACTTATGAACATGGGGTAACTTCATATGATATTGGCACTGGATTTGGACATTTTGCCATTGCTACTCAGGATGTGGGTGAAGTCAATTTCTTCATGGTCTACAAAATGGTTGAAAATGTAAGGGCCAAGGGTGGTGAGAATATGATCATTAGAGAACCTTTTGAGCTGAAAGGCAGCCCTAATTTTTTGCTGGCCTACGTGAAAGATCCTAATGGTTATATCTTTGAGCTCATTCAAAGAGCCCAAACTGAGCCCCTCTGTCATTTAATGCTTCGTGTTGCTGATTTACAACGCTCTATTGACTTCTACCAAAAGGCATTGGGAATGAGGGTACTTACCAAGGTTGAGAGTGTTGAACAGAAGTACGCCATAGCCCTAATGGGATATGCAGATGAGTTAGAGACAACTGCTCTGGAGTTGAATTACAATCATGGTGTTACACAACATTCCAAGGGAAATGGATATTCCCAGGTTGCTATTGGCACCGATGATGTATACAAGAGTGCTGAGATTGTTAATCTCATTACTAAAGAACTTGGTGGAAAGATAATACAACCACCCAGTTTGGATAGCCAAATGAACTCCAAGATTACCAGTTTTCTTGATCCTGATGGCTGGAAAATTGTTCTTGTAGACAATGAAGATTATCTGAAGGGAATGCAGTGA

Protein sequence

MTELNKHVAPDGDEQLLEWPGKDNRRFVRAVYRVGDLDRTIKFYTECLGMKLLRKYEVQNEKHTKALMGFGPEESSFVLELTYEHGVTSYDIGTGFGHFAIATQDVGEVNFFMVYKMVENVRAKGGENMIIREPFELKGSPNFLLAYVKDPNGYIFELIQRAQTEPLCHLMLRVADLQRSIDFYQKALGMRVLTKVESVEQKYAIALMGYADELETTALELNYNHGVTQHSKGNGYSQVAIGTDDVYKSAEIVNLITKELGGKIIQPPSLDSQMNSKITSFLDPDGWKIVLVDNEDYLKGMQ*

Homology

[Close]

BLAST of Chy12G216700 vs. ExPASy Swiss-Prot
Match: O65398 (Lactoylglutathione lyase GLX1 OS=Arabidopsis thaliana OX=3702 GN=GLX1 PE=1 SV=1)

HSP 1 Score: 361.3 bits (926), Expect = 1.1e-98
Identity = 176/288 (61.11%), Postives = 222/288 (77.08%), Query Frame = 0


Query: 16  LLEWPGKDNRRFVRAVYRVGDLDRTIKFYTECLGMKLLRKYEVQNEKHTKALMGFGPEES 75
           LLEWP KDNRRF+  VYRVGDLDRTI+FYTE  GMKLLRK ++  EK++ A +GFGPE S
Sbjct: 7   LLEWPKKDNRRFLHVVYRVGDLDRTIEFYTEVFGMKLLRKRDIPEEKYSNAFLGFGPETS 66

Query: 76  SFVLELTYEHGVTSYDIGTGFGHFAIATQDVGEVNFFMVYKMVENVRAKGGENMIIREPF 135
           +FV+ELTY +GV+SYDIGTGFGHFAI+TQDV         K+VENVRAKGG   + REP 
Sbjct: 67  NFVVELTYNYGVSSYDIGTGFGHFAISTQDVS--------KLVENVRAKGGN--VTREPG 126

Query: 136 ELKGSPNFLLAYVKDPNGYIFELIQRAQT-EPLCHLMLRVADLQRSIDFYQKALGMRVLT 195
            +KG  + ++A+VKDP+GY FELIQR  T EP C +MLRV DL R+I FY+KALGMR+L 
Sbjct: 127 PVKGGGS-VIAFVKDPDGYTFELIQRGPTPEPFCQVMLRVGDLDRAIKFYEKALGMRLLR 186

Query: 196 KVESVEQKYAIALMGYADELETTALELNYNHGVTQHSKGNGYSQVAIGTDDVYKSAEIVN 255
           K+E  E KY I +MGYA+E E+  LEL YN+ VT+++KGN Y+Q+AIGTDDVYKS E++ 
Sbjct: 187 KIERPEYKYTIGMMGYAEEYESIVLELTYNYDVTEYTKGNAYAQIAIGTDDVYKSGEVIK 246

Query: 256 LITKELGGKIIQPPSLDSQMNSKITSFLDPDGWKIVLVDNEDYLKGMQ 303
           ++ +ELGGKI +       + +KI SFLDPDGWK VLVDN+D+LK ++
Sbjct: 247 IVNQELGGKITREAGPLPGLGTKIVSFLDPDGWKTVLVDNKDFLKELE 283

[Close]

BLAST of Chy12G216700 vs. ExPASy Swiss-Prot
Match: Q948T6 (Lactoylglutathione lyase OS=Oryza sativa subsp. japonica OX=39947 GN=GLYI-11 PE=1 SV=2)

HSP 1 Score: 355.5 bits (911), Expect = 5.8e-97
Identity = 176/295 (59.66%), Postives = 223/295 (75.59%), Query Frame = 0


Query: 9   APDGDEQLLEWPGKDNRRFVRAVYRVGDLDRTIKFYTECLGMKLLRKYEVQNEKHTKALM 68
           A    E +LEWP KD +R + AVYRVGDLDRTIK YTEC GMKLLRK +V  EK+T A +
Sbjct: 7   AEKSPEVVLEWPKKDKKRLLHAVYRVGDLDRTIKCYTECFGMKLLRKRDVPEEKYTNAFL 66

Query: 69  GFGPEESSFVLELTYEHGVTSYDIGTGFGHFAIATQDVGEVNFFMVYKMVENVRAKGGEN 128
           GFGPE+++F LELTY +GV  YDIG GFGHFAIAT+D        VYK+ E +++     
Sbjct: 67  GFGPEDTNFALELTYNYGVDKYDIGAGFGHFAIATED--------VYKLAEKIKSSCC-C 126

Query: 129 MIIREPFELKGSPNFLLAYVKDPNGYIFELIQRAQT-EPLCHLMLRVADLQRSIDFYQKA 188
            I REP  +KG  + ++A+ +DP+GY+FELIQR  T EPLC +MLRV DL RSI FY+KA
Sbjct: 127 KITREPGPVKGG-STVIAFAQDPDGYMFELIQRGPTPEPLCQVMLRVGDLDRSIKFYEKA 186

Query: 189 LGMRVLTKVESVEQKYAIALMGYADELETTALELNYNHGVTQHSKGNGYSQVAIGTDDVY 248
           LGM++L K +  + KY IA++GYADE +TT +EL YN+GVT+++KGN Y+QVAIGT+DVY
Sbjct: 187 LGMKLLRKKDVPDYKYTIAMLGYADEDKTTVIELTYNYGVTEYTKGNAYAQVAIGTEDVY 246

Query: 249 KSAEIVNLITKELGGKIIQPPSLDSQMNSKITSFLDPDGWKIVLVDNEDYLKGMQ 303
           KSAE V L+TKELGGKI++ P     +N+KI SFLDPDGWK+VLVDN D+LK +Q
Sbjct: 247 KSAEAVELVTKELGGKILRQPGPLPGLNTKIASFLDPDGWKVVLVDNADFLKELQ 291

[Close]

BLAST of Chy12G216700 vs. ExPASy Swiss-Prot
Match: Q39366 (Putative lactoylglutathione lyase OS=Brassica oleracea var. gemmifera OX=178616 PE=2 SV=1)

HSP 1 Score: 352.4 bits (903), Expect = 5.0e-96
Identity = 175/288 (60.76%), Postives = 221/288 (76.74%), Query Frame = 0


Query: 16  LLEWPGKDNRRFVRAVYRVGDLDRTIKFYTECLGMKLLRKYEVQNEKHTKALMGFGPEES 75
           L+EWP KD RRF+  VYRVGDLDRTI+FYTEC GMK+LRK +V  EK++ A +GFGPE S
Sbjct: 7   LVEWPKKDKRRFLHVVYRVGDLDRTIQFYTECFGMKVLRKRDVPEEKYSNAFLGFGPETS 66

Query: 76  SFVLELTYEHGVTSYDIGTGFGHFAIATQDVGEVNFFMVYKMVENVRAKGGENMIIREPF 135
           +FV+ELTY +GV+SYDIGTGFGHFAI+TQDV         KMVE VRAKGG   + REP 
Sbjct: 67  NFVVELTYNYGVSSYDIGTGFGHFAISTQDVS--------KMVEAVRAKGGN--VTREPG 126

Query: 136 ELKGSPNFLLAYVKDPNGYIFELIQRAQT-EPLCHLMLRVADLQRSIDFYQKALGMRVLT 195
            +KG  + ++A+VKDP+GY FELIQR  T EPLC +MLRV DL R++ F +KALGMR+L 
Sbjct: 127 PVKGGGS-VIAFVKDPDGYTFELIQRGPTPEPLCQVMLRVGDLDRAVKFMEKALGMRLLR 186

Query: 196 KVESVEQKYAIALMGYADELETTALELNYNHGVTQHSKGNGYSQVAIGTDDVYKSAEIVN 255
           ++E  E    I +MGYA+E E+  LEL YN+GVT+++KGN Y+Q+AIGTDDVYKSAE+V 
Sbjct: 187 RIERPEYN-TIGMMGYAEEYESIVLELTYNYGVTEYTKGNAYAQIAIGTDDVYKSAEVVK 246

Query: 256 LITKELGGKIIQPPSLDSQMNSKITSFLDPDGWKIVLVDNEDYLKGMQ 303
           ++ +ELGGKI +       + +KI SFLDPDGWK VLVDNED+LK ++
Sbjct: 247 IVNQELGGKITREAGPLPGLGTKIVSFLDPDGWKQVLVDNEDFLKELE 282

[Close]

BLAST of Chy12G216700 vs. ExPASy Swiss-Prot
Match: Q8W593 (Probable lactoylglutathione lyase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At1g67280 PE=1 SV=1)

HSP 1 Score: 316.6 bits (810), Expect = 3.0e-85
Identity = 160/303 (52.81%), Postives = 207/303 (68.32%), Query Frame = 0


Query: 1   MTELNKHVAPDGDEQLLEWPGKDNRRFVRAVYRVGDLDRTIKFYTECLGMKLLRKYEVQN 60
           + E  K       + LL W   D RR +  VYRVGD+DRTIKFYTECLGMKLLRK ++  
Sbjct: 63  VAESGKAAQATTQDDLLTWVKNDKRRMLHVVYRVGDMDRTIKFYTECLGMKLLRKRDIPE 122

Query: 61  EKHTKALMGFGPEESSFVLELTYEHGVTSYDIGTGFGHFAIATQDVGEVNFFMVYKMVEN 120
           EK+T A +G+GPE+S FV+ELTY +GV  YDIG GFGHF IA  DV         K VE 
Sbjct: 123 EKYTNAFLGYGPEDSHFVIELTYNYGVDKYDIGAGFGHFGIAVDDVA--------KTVEL 182

Query: 121 VRAKGGENMIIREPFELKGSPNFLLAYVKDPNGYIFELIQRAQT-EPLCHLMLRVADLQR 180
           V+AKGG+  + REP  +KG    ++A+++DP+GY FEL++R  T EPLC +MLRV DL R
Sbjct: 183 VKAKGGK--VSREPGPVKGGKT-VIAFIEDPDGYKFELLERGPTPEPLCQVMLRVGDLDR 242

Query: 181 SIDFYQKALGMRVLTKVESVEQKYAIALMGYADELETTALELNYNHGVTQHSKGNGYSQV 240
           +I FY+KA GM +L   ++ E KY IA+MGY  E +   LEL YN+GVT++ KGN Y+Q+
Sbjct: 243 AIKFYEKAFGMELLRTRDNPEYKYTIAMMGYGPEDKFPVLELTYNYGVTEYDKGNAYAQI 302

Query: 241 AIGTDDVYKSAEIVNLITKELGGKIIQPPSLDSQMNSKITSFLDPDGWKIVLVDNEDYLK 300
           AIGTDDVYK+AE + L     GGKI + P     +++KIT+ LDPDGWK V VDN D+LK
Sbjct: 303 AIGTDDVYKTAEAIKL----FGGKITREPGPLPGISTKITACLDPDGWKSVFVDNIDFLK 350

Query: 301 GMQ 303
            ++
Sbjct: 363 ELE 350

[Close]

BLAST of Chy12G216700 vs. ExPASy Swiss-Prot
Match: P46235 (Probable lactoylglutathione lyase OS=Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633) OX=223926 GN=gloA PE=3 SV=2)

HSP 1 Score: 120.2 bits (300), Expect = 4.1e-26
Identity = 62/140 (44.29%), Postives = 85/140 (60.71%), Query Frame = 0


Query: 24  NRRFVRAVYRVGDLDRTIKFYTECLGMKLLRKYEVQNEKHTKALMGFGPEESSFVLELTY 83
           N R +  + RVGDLD++IKFYTE +GM+LLR  E +  ++T A +G+G E    V+ELTY
Sbjct: 3   NGRILHTMLRVGDLDKSIKFYTEVMGMQLLRTNENKEYEYTLAFVGYGDESQGAVIELTY 62

Query: 84  EHGVTSYDIGTGFGHFAIATQDVGEVNFFMVYKMVENVRAKGGENMIIREPFELKGSPNF 143
             G T YD+GT FGH AI   D        +Y   + ++A GG   + RE   +KG    
Sbjct: 63  NWGKTEYDLGTAFGHIAIGVDD--------IYATCDAIKAAGGN--VTREAGPVKGGTTH 122

Query: 144 LLAYVKDPNGYIFELIQRAQ 164
            +A+VKDP+GY+ ELIQ  Q
Sbjct: 123 -IAFVKDPDGYMIELIQNKQ 131

[Close]

BLAST of Chy12G216700 vs. ExPASy TrEMBL
Match: A0A0A0LS05 (Glyoxalase I OS=Cucumis sativus OX=3659 GN=Csa_1G062880 PE=3 SV=1)

HSP 1 Score: 574.3 bits (1479), Expect = 3.0e-160
Identity = 282/302 (93.38%), Postives = 289/302 (95.70%), Query Frame = 0


Query: 1   MTELNKHVAPDGDEQLLEWPGKDNRRFVRAVYRVGDLDRTIKFYTECLGMKLLRKYEVQN 60
           MTELNKHVAPDGDEQLLEWPGKDNRRFVRAVYRVGDLDRTIKFYTECL MKLLRKYEV N
Sbjct: 1   MTELNKHVAPDGDEQLLEWPGKDNRRFVRAVYRVGDLDRTIKFYTECLEMKLLRKYEVPN 60

Query: 61  EKHTKALMGFGPEESSFVLELTYEHGVTSYDIGTGFGHFAIATQDVGEVNFFMVYKMVEN 120
           EKHTKALMGFGPEESSF+LELT+EHGVTSYDIGTGFGHFAIATQD        VYKMVEN
Sbjct: 61  EKHTKALMGFGPEESSFILELTHEHGVTSYDIGTGFGHFAIATQD--------VYKMVEN 120

Query: 121 VRAKGGENMIIREPFELKGSPNFLLAYVKDPNGYIFELIQRAQTEPLCHLMLRVADLQRS 180
           VRAKGGENMIIREPFELKGSPNFLLAYVKDPNGYIFELIQR QT+PLCHLMLRVADLQRS
Sbjct: 121 VRAKGGENMIIREPFELKGSPNFLLAYVKDPNGYIFELIQRVQTQPLCHLMLRVADLQRS 180

Query: 181 IDFYQKALGMRVLTKVESVEQKYAIALMGYADELETTALELNYNHGVTQHSKGNGYSQVA 240
           IDFYQKA+GMRVLT+VESVEQKYAIALMGYADELETTALELNYNHGVTQHSKGNGYSQV 
Sbjct: 181 IDFYQKAMGMRVLTQVESVEQKYAIALMGYADELETTALELNYNHGVTQHSKGNGYSQVV 240

Query: 241 IGTDDVYKSAEIVNLITKELGGKIIQPPSLDSQMNSKITSFLDPDGWKIVLVDNEDYLKG 300
           IGTDDVYKSAEIVNLITKELGG+I QPPSLDSQ+NSKITSFLDPDGWKIVLVDNEDYLKG
Sbjct: 241 IGTDDVYKSAEIVNLITKELGGEITQPPSLDSQINSKITSFLDPDGWKIVLVDNEDYLKG 294

Query: 301 MQ 303
           MQ
Sbjct: 301 MQ 294

[Close]

BLAST of Chy12G216700 vs. ExPASy TrEMBL
Match: A0A5D3BI05 (Glyoxalase I OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G005690 PE=3 SV=1)

HSP 1 Score: 544.3 bits (1401), Expect = 3.3e-151
Identity = 274/303 (90.43%), Postives = 282/303 (93.07%), Query Frame = 0


Query: 1   MTELNKHVAPDGDEQLLEWPGKDNRRFVRAVYRVGDLDRTIKFYTECLGMKLLRKYEVQN 60
           MTE  K VAPDGDEQLLEWP KDNRRFVRAVYRVGDLDRTIKFYTECLGM+LLRKYEV N
Sbjct: 1   MTE-TKPVAPDGDEQLLEWPNKDNRRFVRAVYRVGDLDRTIKFYTECLGMELLRKYEVSN 60

Query: 61  EKHTKALMGFGPEESSFVLELTYEHGVTSYDIGTGFGHFAIATQDVGEVNFFMVYKMVEN 120
           EKHTKA+MGFGPEESSFVLELTYE GVTSYDIGTGFGHFAIATQD        VYKMVEN
Sbjct: 61  EKHTKAIMGFGPEESSFVLELTYEDGVTSYDIGTGFGHFAIATQD--------VYKMVEN 120

Query: 121 VRAKGGENMIIREPFELKGSPNFLLAYVKDPNGYIFELIQRAQT-EPLCHLMLRVADLQR 180
           VRAKGGENMIIREPFELKGSP  LLAYVKDPNGYIFELIQR QT +PLCHLMLRVADLQR
Sbjct: 121 VRAKGGENMIIREPFELKGSPVLLLAYVKDPNGYIFELIQRGQTPQPLCHLMLRVADLQR 180

Query: 181 SIDFYQKALGMRVLTKVESVEQKYAIALMGYADELETTALELNYNHGVTQHSKGNGYSQV 240
           SIDFY+KALGMRVLTKVES+EQKYAIALMGYADELETTA+EL YNHGVTQHSKGNGYSQV
Sbjct: 181 SIDFYRKALGMRVLTKVESLEQKYAIALMGYADELETTAVELTYNHGVTQHSKGNGYSQV 240

Query: 241 AIGTDDVYKSAEIVNLITKELGGKIIQPPSLDSQMNSKITSFLDPDGWKIVLVDNEDYLK 300
           AIGTDDVYKSAEIVNLITK+LGGKI QPPSLDSQMNSKI SFLDPDGW+IVLVDNEDYLK
Sbjct: 241 AIGTDDVYKSAEIVNLITKKLGGKITQPPSLDSQMNSKIISFLDPDGWQIVLVDNEDYLK 294

Query: 301 GMQ 303
           GMQ
Sbjct: 301 GMQ 294

[Close]

BLAST of Chy12G216700 vs. ExPASy TrEMBL
Match: E5GC58 (Glyoxalase I OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 544.3 bits (1401), Expect = 3.3e-151
Identity = 274/303 (90.43%), Postives = 282/303 (93.07%), Query Frame = 0


Query: 1   MTELNKHVAPDGDEQLLEWPGKDNRRFVRAVYRVGDLDRTIKFYTECLGMKLLRKYEVQN 60
           MTE  K VAPDGDEQLLEWP KDNRRFVRAVYRVGDLDRTIKFYTECLGM+LLRKYEV N
Sbjct: 1   MTE-TKPVAPDGDEQLLEWPNKDNRRFVRAVYRVGDLDRTIKFYTECLGMELLRKYEVSN 60

Query: 61  EKHTKALMGFGPEESSFVLELTYEHGVTSYDIGTGFGHFAIATQDVGEVNFFMVYKMVEN 120
           EKHTKA+MGFGPEESSFVLELTYE GVTSYDIGTGFGHFAIATQD        VYKMVEN
Sbjct: 61  EKHTKAIMGFGPEESSFVLELTYEDGVTSYDIGTGFGHFAIATQD--------VYKMVEN 120

Query: 121 VRAKGGENMIIREPFELKGSPNFLLAYVKDPNGYIFELIQRAQT-EPLCHLMLRVADLQR 180
           VRAKGGENMIIREPFELKGSP  LLAYVKDPNGYIFELIQR QT +PLCHLMLRVADLQR
Sbjct: 121 VRAKGGENMIIREPFELKGSPVLLLAYVKDPNGYIFELIQRGQTPQPLCHLMLRVADLQR 180

Query: 181 SIDFYQKALGMRVLTKVESVEQKYAIALMGYADELETTALELNYNHGVTQHSKGNGYSQV 240
           SIDFY+KALGMRVLTKVES+EQKYAIALMGYADELETTA+EL YNHGVTQHSKGNGYSQV
Sbjct: 181 SIDFYRKALGMRVLTKVESLEQKYAIALMGYADELETTAVELTYNHGVTQHSKGNGYSQV 240

Query: 241 AIGTDDVYKSAEIVNLITKELGGKIIQPPSLDSQMNSKITSFLDPDGWKIVLVDNEDYLK 300
           AIGTDDVYKSAEIVNLITK+LGGKI QPPSLDSQMNSKI SFLDPDGW+IVLVDNEDYLK
Sbjct: 241 AIGTDDVYKSAEIVNLITKKLGGKITQPPSLDSQMNSKIISFLDPDGWQIVLVDNEDYLK 294

Query: 301 GMQ 303
           GMQ
Sbjct: 301 GMQ 294

[Close]

BLAST of Chy12G216700 vs. ExPASy TrEMBL
Match: A0A1S3B2D8 (Glyoxalase I OS=Cucumis melo OX=3656 GN=LOC103485234 PE=3 SV=1)

HSP 1 Score: 544.3 bits (1401), Expect = 3.3e-151
Identity = 274/303 (90.43%), Postives = 282/303 (93.07%), Query Frame = 0


Query: 1   MTELNKHVAPDGDEQLLEWPGKDNRRFVRAVYRVGDLDRTIKFYTECLGMKLLRKYEVQN 60
           MTE  K VAPDGDEQLLEWP KDNRRFVRAVYRVGDLDRTIKFYTECLGM+LLRKYEV N
Sbjct: 1   MTE-TKPVAPDGDEQLLEWPNKDNRRFVRAVYRVGDLDRTIKFYTECLGMELLRKYEVSN 60

Query: 61  EKHTKALMGFGPEESSFVLELTYEHGVTSYDIGTGFGHFAIATQDVGEVNFFMVYKMVEN 120
           EKHTKA+MGFGPEESSFVLELTYE GVTSYDIGTGFGHFAIATQD        VYKMVEN
Sbjct: 61  EKHTKAIMGFGPEESSFVLELTYEDGVTSYDIGTGFGHFAIATQD--------VYKMVEN 120

Query: 121 VRAKGGENMIIREPFELKGSPNFLLAYVKDPNGYIFELIQRAQT-EPLCHLMLRVADLQR 180
           VRAKGGENMIIREPFELKGSP  LLAYVKDPNGYIFELIQR QT +PLCHLMLRVADLQR
Sbjct: 121 VRAKGGENMIIREPFELKGSPVLLLAYVKDPNGYIFELIQRGQTPQPLCHLMLRVADLQR 180

Query: 181 SIDFYQKALGMRVLTKVESVEQKYAIALMGYADELETTALELNYNHGVTQHSKGNGYSQV 240
           SIDFY+KALGMRVLTKVES+EQKYAIALMGYADELETTA+EL YNHGVTQHSKGNGYSQV
Sbjct: 181 SIDFYRKALGMRVLTKVESLEQKYAIALMGYADELETTAVELTYNHGVTQHSKGNGYSQV 240

Query: 241 AIGTDDVYKSAEIVNLITKELGGKIIQPPSLDSQMNSKITSFLDPDGWKIVLVDNEDYLK 300
           AIGTDDVYKSAEIVNLITK+LGGKI QPPSLDSQMNSKI SFLDPDGW+IVLVDNEDYLK
Sbjct: 241 AIGTDDVYKSAEIVNLITKKLGGKITQPPSLDSQMNSKIISFLDPDGWQIVLVDNEDYLK 294

Query: 301 GMQ 303
           GMQ
Sbjct: 301 GMQ 294

[Close]

BLAST of Chy12G216700 vs. ExPASy TrEMBL
Match: A0A6J1CEU9 (Lactoylglutathione lyase OS=Momordica charantia OX=3673 GN=LOC111010851 PE=3 SV=1)

HSP 1 Score: 457.2 bits (1175), Expect = 5.3e-125
Identity = 232/303 (76.57%), Postives = 255/303 (84.16%), Query Frame = 0


Query: 1   MTELNKHVAPDGDEQLLEWPGKDNRRFVRAVYRVGDLDRTIKFYTECLGMKLLRKYEVQN 60
           M E    +APD D QLLEWP KDNRR + AVY VGDLDRTIKFYTEC GMKLLR  +V  
Sbjct: 1   MAEQATPLAPDADAQLLEWPKKDNRRLLHAVYCVGDLDRTIKFYTECFGMKLLRIRDVPE 60

Query: 61  EKHTKALMGFGPEESSFVLELTYEHGVTSYDIGTGFGHFAIATQDVGEVNFFMVYKMVEN 120
           EK++ A MGFGPE+SSFV+EL Y HGVTSYDIGTGFGHFAIATQD        VYKMVEN
Sbjct: 61  EKYSNAFMGFGPEQSSFVVELIYNHGVTSYDIGTGFGHFAIATQD--------VYKMVEN 120

Query: 121 VRAKGGENMIIREPFELKGSPNFLLAYVKDPNGYIFELIQRAQT-EPLCHLMLRVADLQR 180
           + AK  ++ IIREPFELKGS +F+LAYVKDP+GYIFELIQRA T +PLCH+MLRVADLQR
Sbjct: 121 ITAKSADS-IIREPFELKGS-SFVLAYVKDPDGYIFELIQRAPTPQPLCHVMLRVADLQR 180

Query: 181 SIDFYQKALGMRVLTKVESVEQKYAIALMGYADELETTALELNYNHGVTQHSKGNGYSQV 240
           SI+FY+KALGMR L KVESVE KYAIA+MGYADE ET ALEL YNHGVT+HSKGNGYSQV
Sbjct: 181 SINFYEKALGMRSLNKVESVEHKYAIAIMGYADESETVALELTYNHGVTEHSKGNGYSQV 240

Query: 241 AIGTDDVYKSAEIVNLITKELGGKIIQPPSLDSQMNSKITSFLDPDGWKIVLVDNEDYLK 300
           AIGTDDVYKSAE+VNLITKELGGKI Q PSL   +N+KIT FLDPDGWKIVLVDNEDYLK
Sbjct: 241 AIGTDDVYKSAEVVNLITKELGGKITQQPSLIHPINAKITEFLDPDGWKIVLVDNEDYLK 293

Query: 301 GMQ 303
            +Q
Sbjct: 301 ELQ 293

[Close]

BLAST of Chy12G216700 vs. NCBI nr
Match: XP_004145118.1 (lactoylglutathione lyase GLX1 [Cucumis sativus] >KGN64513.1 hypothetical protein Csa_013535 [Cucumis sativus])

HSP 1 Score: 570 bits (1469), Expect = 1.52e-204
Identity = 282/302 (93.38%), Postives = 289/302 (95.70%), Query Frame = 0


Query: 1   MTELNKHVAPDGDEQLLEWPGKDNRRFVRAVYRVGDLDRTIKFYTECLGMKLLRKYEVQN 60
           MTELNKHVAPDGDEQLLEWPGKDNRRFVRAVYRVGDLDRTIKFYTECL MKLLRKYEV N
Sbjct: 1   MTELNKHVAPDGDEQLLEWPGKDNRRFVRAVYRVGDLDRTIKFYTECLEMKLLRKYEVPN 60

Query: 61  EKHTKALMGFGPEESSFVLELTYEHGVTSYDIGTGFGHFAIATQDVGEVNFFMVYKMVEN 120
           EKHTKALMGFGPEESSF+LELT+EHGVTSYDIGTGFGHFAIATQDV        YKMVEN
Sbjct: 61  EKHTKALMGFGPEESSFILELTHEHGVTSYDIGTGFGHFAIATQDV--------YKMVEN 120

Query: 121 VRAKGGENMIIREPFELKGSPNFLLAYVKDPNGYIFELIQRAQTEPLCHLMLRVADLQRS 180
           VRAKGGENMIIREPFELKGSPNFLLAYVKDPNGYIFELIQR QT+PLCHLMLRVADLQRS
Sbjct: 121 VRAKGGENMIIREPFELKGSPNFLLAYVKDPNGYIFELIQRVQTQPLCHLMLRVADLQRS 180

Query: 181 IDFYQKALGMRVLTKVESVEQKYAIALMGYADELETTALELNYNHGVTQHSKGNGYSQVA 240
           IDFYQKA+GMRVLT+VESVEQKYAIALMGYADELETTALELNYNHGVTQHSKGNGYSQV 
Sbjct: 181 IDFYQKAMGMRVLTQVESVEQKYAIALMGYADELETTALELNYNHGVTQHSKGNGYSQVV 240

Query: 241 IGTDDVYKSAEIVNLITKELGGKIIQPPSLDSQMNSKITSFLDPDGWKIVLVDNEDYLKG 300
           IGTDDVYKSAEIVNLITKELGG+I QPPSLDSQ+NSKITSFLDPDGWKIVLVDNEDYLKG
Sbjct: 241 IGTDDVYKSAEIVNLITKELGGEITQPPSLDSQINSKITSFLDPDGWKIVLVDNEDYLKG 294

Query: 301 MQ 302
           MQ
Sbjct: 301 MQ 294

[Close]

BLAST of Chy12G216700 vs. NCBI nr
Match: XP_008440988.1 (PREDICTED: putative lactoylglutathione lyase [Cucumis melo] >ADN34057.1 lactoylglutathione lyase [Cucumis melo subsp. melo] >KAA0056849.1 putative lactoylglutathione lyase [Cucumis melo var. makuwa] >TYJ99352.1 putative lactoylglutathione lyase [Cucumis melo var. makuwa])

HSP 1 Score: 540 bits (1391), Expect = 1.17e-192
Identity = 274/303 (90.43%), Postives = 282/303 (93.07%), Query Frame = 0


Query: 1   MTELNKHVAPDGDEQLLEWPGKDNRRFVRAVYRVGDLDRTIKFYTECLGMKLLRKYEVQN 60
           MTE  K VAPDGDEQLLEWP KDNRRFVRAVYRVGDLDRTIKFYTECLGM+LLRKYEV N
Sbjct: 1   MTET-KPVAPDGDEQLLEWPNKDNRRFVRAVYRVGDLDRTIKFYTECLGMELLRKYEVSN 60

Query: 61  EKHTKALMGFGPEESSFVLELTYEHGVTSYDIGTGFGHFAIATQDVGEVNFFMVYKMVEN 120
           EKHTKA+MGFGPEESSFVLELTYE GVTSYDIGTGFGHFAIATQDV        YKMVEN
Sbjct: 61  EKHTKAIMGFGPEESSFVLELTYEDGVTSYDIGTGFGHFAIATQDV--------YKMVEN 120

Query: 121 VRAKGGENMIIREPFELKGSPNFLLAYVKDPNGYIFELIQRAQT-EPLCHLMLRVADLQR 180
           VRAKGGENMIIREPFELKGSP  LLAYVKDPNGYIFELIQR QT +PLCHLMLRVADLQR
Sbjct: 121 VRAKGGENMIIREPFELKGSPVLLLAYVKDPNGYIFELIQRGQTPQPLCHLMLRVADLQR 180

Query: 181 SIDFYQKALGMRVLTKVESVEQKYAIALMGYADELETTALELNYNHGVTQHSKGNGYSQV 240
           SIDFY+KALGMRVLTKVES+EQKYAIALMGYADELETTA+EL YNHGVTQHSKGNGYSQV
Sbjct: 181 SIDFYRKALGMRVLTKVESLEQKYAIALMGYADELETTAVELTYNHGVTQHSKGNGYSQV 240

Query: 241 AIGTDDVYKSAEIVNLITKELGGKIIQPPSLDSQMNSKITSFLDPDGWKIVLVDNEDYLK 300
           AIGTDDVYKSAEIVNLITK+LGGKI QPPSLDSQMNSKI SFLDPDGW+IVLVDNEDYLK
Sbjct: 241 AIGTDDVYKSAEIVNLITKKLGGKITQPPSLDSQMNSKIISFLDPDGWQIVLVDNEDYLK 294

Query: 301 GMQ 302
           GMQ
Sbjct: 301 GMQ 294

[Close]

BLAST of Chy12G216700 vs. NCBI nr
Match: XP_038895603.1 (LOW QUALITY PROTEIN: lactoylglutathione lyase GLX1 [Benincasa hispida])

HSP 1 Score: 478 bits (1230), Expect = 3.95e-168
Identity = 238/296 (80.41%), Postives = 261/296 (88.18%), Query Frame = 0


Query: 8   VAPDGDEQLLEWPGKDNRRFVRAVYRVGDLDRTIKFYTECLGMKLLRKYEVQNEKHTKAL 67
           VAPDG +Q+LEWP KDNRRF+R VYRVGDLDRTIKFYTEC GM+LLRK EVQ EKHTKA 
Sbjct: 7   VAPDGHDQMLEWPKKDNRRFLRTVYRVGDLDRTIKFYTECFGMELLRKNEVQEEKHTKAF 66

Query: 68  MGFGPEESSFVLELTYEHGVTSYDIGTGFGHFAIATQDVGEVNFFMVYKMVENVRAKGGE 127
           MG+GPE+SS VLEL YEHGVTSY+IG GFGHFAIATQDV        YKMVENV+ KGG+
Sbjct: 67  MGYGPEQSSIVLELIYEHGVTSYNIGNGFGHFAIATQDV--------YKMVENVKDKGGD 126

Query: 128 NMIIREPFELKGSPNFLLAYVKDPNGYIFELIQRAQT-EPLCHLMLRVADLQRSIDFYQK 187
           NMIIREPFE+KGS  F+LAYVKDP+GYIFELIQR  T +PLCH+MLRV DLQRSIDFY+K
Sbjct: 127 NMIIREPFEVKGSSIFVLAYVKDPDGYIFELIQRDPTPQPLCHVMLRVGDLQRSIDFYKK 186

Query: 188 ALGMRVLTKVESVEQKYAIALMGYADELETTALELNYNHGVTQHSKGNGYSQVAIGTDDV 247
           ALGMRVL KV+S+E KYAIA+MGYADELETTALEL YNHGVT+HSKG  YSQVAIGTDDV
Sbjct: 187 ALGMRVLNKVQSLEHKYAIAMMGYADELETTALELTYNHGVTEHSKGMXYSQVAIGTDDV 246

Query: 248 YKSAEIVNLITKELGGKIIQPPSLDSQMNSKITSFLDPDGWKIVLVDNEDYLKGMQ 302
           YKSAE+VNLITKELGGKI Q PSL SQ NSKITSFLDPDGW+IVLVDNEDY+K +Q
Sbjct: 247 YKSAEVVNLITKELGGKINQQPSLASQTNSKITSFLDPDGWQIVLVDNEDYMKELQ 294

[Close]

BLAST of Chy12G216700 vs. NCBI nr
Match: XP_022140116.1 (putative lactoylglutathione lyase [Momordica charantia])

HSP 1 Score: 453 bits (1165), Expect = 3.01e-158
Identity = 232/303 (76.57%), Postives = 255/303 (84.16%), Query Frame = 0


Query: 1   MTELNKHVAPDGDEQLLEWPGKDNRRFVRAVYRVGDLDRTIKFYTECLGMKLLRKYEVQN 60
           M E    +APD D QLLEWP KDNRR + AVY VGDLDRTIKFYTEC GMKLLR  +V  
Sbjct: 1   MAEQATPLAPDADAQLLEWPKKDNRRLLHAVYCVGDLDRTIKFYTECFGMKLLRIRDVPE 60

Query: 61  EKHTKALMGFGPEESSFVLELTYEHGVTSYDIGTGFGHFAIATQDVGEVNFFMVYKMVEN 120
           EK++ A MGFGPE+SSFV+EL Y HGVTSYDIGTGFGHFAIATQDV        YKMVEN
Sbjct: 61  EKYSNAFMGFGPEQSSFVVELIYNHGVTSYDIGTGFGHFAIATQDV--------YKMVEN 120

Query: 121 VRAKGGENMIIREPFELKGSPNFLLAYVKDPNGYIFELIQRAQT-EPLCHLMLRVADLQR 180
           + AK  ++ IIREPFELKGS +F+LAYVKDP+GYIFELIQRA T +PLCH+MLRVADLQR
Sbjct: 121 ITAKSADS-IIREPFELKGS-SFVLAYVKDPDGYIFELIQRAPTPQPLCHVMLRVADLQR 180

Query: 181 SIDFYQKALGMRVLTKVESVEQKYAIALMGYADELETTALELNYNHGVTQHSKGNGYSQV 240
           SI+FY+KALGMR L KVESVE KYAIA+MGYADE ET ALEL YNHGVT+HSKGNGYSQV
Sbjct: 181 SINFYEKALGMRSLNKVESVEHKYAIAIMGYADESETVALELTYNHGVTEHSKGNGYSQV 240

Query: 241 AIGTDDVYKSAEIVNLITKELGGKIIQPPSLDSQMNSKITSFLDPDGWKIVLVDNEDYLK 300
           AIGTDDVYKSAE+VNLITKELGGKI Q PSL   +N+KIT FLDPDGWKIVLVDNEDYLK
Sbjct: 241 AIGTDDVYKSAEVVNLITKELGGKITQQPSLIHPINAKITEFLDPDGWKIVLVDNEDYLK 293

Query: 301 GMQ 302
            +Q
Sbjct: 301 ELQ 293

[Close]

BLAST of Chy12G216700 vs. NCBI nr
Match: XP_022994208.1 (LOW QUALITY PROTEIN: lactoylglutathione lyase GLX1 [Cucurbita maxima])

HSP 1 Score: 425 bits (1093), Expect = 1.49e-146
Identity = 216/301 (71.76%), Postives = 249/301 (82.72%), Query Frame = 0


Query: 3   ELNKHVAPDGDEQLLEWPGKDNRRFVRAVYRVGDLDRTIKFYTECLGMKLLRKYEVQNEK 62
           ++ + VAPD DEQLLEWP KDNR F+RAVYRV DLDRTI+FYTEC GMKLLR  +V   K
Sbjct: 49  QMAEVVAPDADEQLLEWPMKDNRPFLRAVYRVSDLDRTIEFYTECFGMKLLRIRDVPEAK 108

Query: 63  HTKALMGFGPEESSFVLELTYEHGVTSYDIGTGFGHFAIATQDVGEVNFFMVYKMVENVR 122
            T+A MGFG E+SSFVLEL Y +GVTSYDIG GFGHFAIA+QDV        YKMVENVR
Sbjct: 109 CTRAFMGFGSEQSSFVLELNYSYGVTSYDIGAGFGHFAIASQDV--------YKMVENVR 168

Query: 123 AKG-GENMIIREPFELKGSPNFLLAYVKDPNGYIFELIQRAQT-EPLCHLMLRVADLQRS 182
            KG G   ++RE FE+KG  + +LA+VKDP+GYIFELIQR  T +PLC +MLRV DL+RS
Sbjct: 169 VKGAGAGAVVRESFEVKGF-SIVLAFVKDPDGYIFELIQRGPTPQPLCQVMLRVGDLERS 228

Query: 183 IDFYQKALGMRVLTKVESVEQKYAIALMGYADELETTALELNYNHGVTQHSKGNGYSQVA 242
           I+FY+KALGMRVLT++ES+E KYAIA+MGYADELETT LEL YNHGVTQHSKGNGYSQVA
Sbjct: 229 INFYEKALGMRVLTRIESLEHKYAIAMMGYADELETTVLELTYNHGVTQHSKGNGYSQVA 288

Query: 243 IGTDDVYKSAEIVNLITKELGGKIIQPPSLDSQMNSKITSFLDPDGWKIVLVDNEDYLKG 301
           IGTD V KSAE++NLI +ELGGKI Q PSL  Q  +KIT+FLDPDGW+IVLVDNEDYLKG
Sbjct: 289 IGTDKVNKSAEVLNLIIQELGGKITQQPSLVHQFKAKITTFLDPDGWQIVLVDNEDYLKG 340

[Close]

BLAST of Chy12G216700 vs. TAIR 10
Match: AT1G11840.6 (glyoxalase I homolog )

HSP 1 Score: 363.2 bits (931), Expect = 2.0e-100
Identity = 179/300 (59.67%), Postives = 229/300 (76.33%), Query Frame = 0


Query: 4   LNKHVAPDGDEQLLEWPGKDNRRFVRAVYRVGDLDRTIKFYTECLGMKLLRKYEVQNEKH 63
           L++++A   D  LLEWP KDNRRF+  VYRVGDLDRTI+FYTE  GMKLLRK ++  EK+
Sbjct: 36  LSRNMAEASD--LLEWPKKDNRRFLHVVYRVGDLDRTIEFYTEVFGMKLLRKRDIPEEKY 95

Query: 64  TKALMGFGPEESSFVLELTYEHGVTSYDIGTGFGHFAIATQDVGEVNFFMVYKMVENVRA 123
           + A +GFGPE S+FV+ELTY +GV+SYDIGTGFGHFAI+TQDV         K+VENVRA
Sbjct: 96  SNAFLGFGPETSNFVVELTYNYGVSSYDIGTGFGHFAISTQDVS--------KLVENVRA 155

Query: 124 KGGENMIIREPFELKGSPNFLLAYVKDPNGYIFELIQRAQT-EPLCHLMLRVADLQRSID 183
           KGG   + REP  +KG  + ++A+VKDP+GY FELIQR  T EP C +MLRV DL R+I 
Sbjct: 156 KGGN--VTREPGPVKGGGS-VIAFVKDPDGYTFELIQRGPTPEPFCQVMLRVGDLDRAIK 215

Query: 184 FYQKALGMRVLTKVESVEQKYAIALMGYADELETTALELNYNHGVTQHSKGNGYSQVAIG 243
           FY+KALGMR+L K+E  E KY I +MGYA+E E+  LEL YN+ VT+++KGN Y+Q+AIG
Sbjct: 216 FYEKALGMRLLRKIERPEYKYTIGMMGYAEEYESIVLELTYNYDVTEYTKGNAYAQIAIG 275

Query: 244 TDDVYKSAEIVNLITKELGGKIIQPPSLDSQMNSKITSFLDPDGWKIVLVDNEDYLKGMQ 303
           TDDVYKS E++ ++ +ELGGKI +       + +KI SFLDPDGWK VLVDN+D+LK ++
Sbjct: 276 TDDVYKSGEVIKIVNQELGGKITREAGPLPGLGTKIVSFLDPDGWKTVLVDNKDFLKELE 322

[Close]

BLAST of Chy12G216700 vs. TAIR 10
Match: AT1G11840.2 (glyoxalase I homolog )

HSP 1 Score: 361.3 bits (926), Expect = 7.6e-100
Identity = 176/288 (61.11%), Postives = 222/288 (77.08%), Query Frame = 0


Query: 16  LLEWPGKDNRRFVRAVYRVGDLDRTIKFYTECLGMKLLRKYEVQNEKHTKALMGFGPEES 75
           LLEWP KDNRRF+  VYRVGDLDRTI+FYTE  GMKLLRK ++  EK++ A +GFGPE S
Sbjct: 7   LLEWPKKDNRRFLHVVYRVGDLDRTIEFYTEVFGMKLLRKRDIPEEKYSNAFLGFGPETS 66

Query: 76  SFVLELTYEHGVTSYDIGTGFGHFAIATQDVGEVNFFMVYKMVENVRAKGGENMIIREPF 135
           +FV+ELTY +GV+SYDIGTGFGHFAI+TQDV         K+VENVRAKGG   + REP 
Sbjct: 67  NFVVELTYNYGVSSYDIGTGFGHFAISTQDVS--------KLVENVRAKGGN--VTREPG 126

Query: 136 ELKGSPNFLLAYVKDPNGYIFELIQRAQT-EPLCHLMLRVADLQRSIDFYQKALGMRVLT 195
            +KG  + ++A+VKDP+GY FELIQR  T EP C +MLRV DL R+I FY+KALGMR+L 
Sbjct: 127 PVKGGGS-VIAFVKDPDGYTFELIQRGPTPEPFCQVMLRVGDLDRAIKFYEKALGMRLLR 186

Query: 196 KVESVEQKYAIALMGYADELETTALELNYNHGVTQHSKGNGYSQVAIGTDDVYKSAEIVN 255
           K+E  E KY I +MGYA+E E+  LEL YN+ VT+++KGN Y+Q+AIGTDDVYKS E++ 
Sbjct: 187 KIERPEYKYTIGMMGYAEEYESIVLELTYNYDVTEYTKGNAYAQIAIGTDDVYKSGEVIK 246

Query: 256 LITKELGGKIIQPPSLDSQMNSKITSFLDPDGWKIVLVDNEDYLKGMQ 303
           ++ +ELGGKI +       + +KI SFLDPDGWK VLVDN+D+LK ++
Sbjct: 247 IVNQELGGKITREAGPLPGLGTKIVSFLDPDGWKTVLVDNKDFLKELE 283

[Close]

BLAST of Chy12G216700 vs. TAIR 10
Match: AT1G11840.3 (glyoxalase I homolog )

HSP 1 Score: 361.3 bits (926), Expect = 7.6e-100
Identity = 176/288 (61.11%), Postives = 222/288 (77.08%), Query Frame = 0


Query: 16  LLEWPGKDNRRFVRAVYRVGDLDRTIKFYTECLGMKLLRKYEVQNEKHTKALMGFGPEES 75
           LLEWP KDNRRF+  VYRVGDLDRTI+FYTE  GMKLLRK ++  EK++ A +GFGPE S
Sbjct: 7   LLEWPKKDNRRFLHVVYRVGDLDRTIEFYTEVFGMKLLRKRDIPEEKYSNAFLGFGPETS 66

Query: 76  SFVLELTYEHGVTSYDIGTGFGHFAIATQDVGEVNFFMVYKMVENVRAKGGENMIIREPF 135
           +FV+ELTY +GV+SYDIGTGFGHFAI+TQDV         K+VENVRAKGG   + REP 
Sbjct: 67  NFVVELTYNYGVSSYDIGTGFGHFAISTQDVS--------KLVENVRAKGGN--VTREPG 126

Query: 136 ELKGSPNFLLAYVKDPNGYIFELIQRAQT-EPLCHLMLRVADLQRSIDFYQKALGMRVLT 195
            +KG  + ++A+VKDP+GY FELIQR  T EP C +MLRV DL R+I FY+KALGMR+L 
Sbjct: 127 PVKGGGS-VIAFVKDPDGYTFELIQRGPTPEPFCQVMLRVGDLDRAIKFYEKALGMRLLR 186

Query: 196 KVESVEQKYAIALMGYADELETTALELNYNHGVTQHSKGNGYSQVAIGTDDVYKSAEIVN 255
           K+E  E KY I +MGYA+E E+  LEL YN+ VT+++KGN Y+Q+AIGTDDVYKS E++ 
Sbjct: 187 KIERPEYKYTIGMMGYAEEYESIVLELTYNYDVTEYTKGNAYAQIAIGTDDVYKSGEVIK 246

Query: 256 LITKELGGKIIQPPSLDSQMNSKITSFLDPDGWKIVLVDNEDYLKGMQ 303
           ++ +ELGGKI +       + +KI SFLDPDGWK VLVDN+D+LK ++
Sbjct: 247 IVNQELGGKITREAGPLPGLGTKIVSFLDPDGWKTVLVDNKDFLKELE 283

[Close]

BLAST of Chy12G216700 vs. TAIR 10
Match: AT1G11840.1 (glyoxalase I homolog )

HSP 1 Score: 361.3 bits (926), Expect = 7.6e-100
Identity = 176/288 (61.11%), Postives = 222/288 (77.08%), Query Frame = 0


Query: 16  LLEWPGKDNRRFVRAVYRVGDLDRTIKFYTECLGMKLLRKYEVQNEKHTKALMGFGPEES 75
           LLEWP KDNRRF+  VYRVGDLDRTI+FYTE  GMKLLRK ++  EK++ A +GFGPE S
Sbjct: 7   LLEWPKKDNRRFLHVVYRVGDLDRTIEFYTEVFGMKLLRKRDIPEEKYSNAFLGFGPETS 66

Query: 76  SFVLELTYEHGVTSYDIGTGFGHFAIATQDVGEVNFFMVYKMVENVRAKGGENMIIREPF 135
           +FV+ELTY +GV+SYDIGTGFGHFAI+TQDV         K+VENVRAKGG   + REP 
Sbjct: 67  NFVVELTYNYGVSSYDIGTGFGHFAISTQDVS--------KLVENVRAKGGN--VTREPG 126

Query: 136 ELKGSPNFLLAYVKDPNGYIFELIQRAQT-EPLCHLMLRVADLQRSIDFYQKALGMRVLT 195
            +KG  + ++A+VKDP+GY FELIQR  T EP C +MLRV DL R+I FY+KALGMR+L 
Sbjct: 127 PVKGGGS-VIAFVKDPDGYTFELIQRGPTPEPFCQVMLRVGDLDRAIKFYEKALGMRLLR 186

Query: 196 KVESVEQKYAIALMGYADELETTALELNYNHGVTQHSKGNGYSQVAIGTDDVYKSAEIVN 255
           K+E  E KY I +MGYA+E E+  LEL YN+ VT+++KGN Y+Q+AIGTDDVYKS E++ 
Sbjct: 187 KIERPEYKYTIGMMGYAEEYESIVLELTYNYDVTEYTKGNAYAQIAIGTDDVYKSGEVIK 246

Query: 256 LITKELGGKIIQPPSLDSQMNSKITSFLDPDGWKIVLVDNEDYLKGMQ 303
           ++ +ELGGKI +       + +KI SFLDPDGWK VLVDN+D+LK ++
Sbjct: 247 IVNQELGGKITREAGPLPGLGTKIVSFLDPDGWKTVLVDNKDFLKELE 283

[Close]

BLAST of Chy12G216700 vs. TAIR 10
Match: AT1G11840.4 (glyoxalase I homolog )

HSP 1 Score: 361.3 bits (926), Expect = 7.6e-100
Identity = 176/288 (61.11%), Postives = 222/288 (77.08%), Query Frame = 0


Query: 16  LLEWPGKDNRRFVRAVYRVGDLDRTIKFYTECLGMKLLRKYEVQNEKHTKALMGFGPEES 75
           LLEWP KDNRRF+  VYRVGDLDRTI+FYTE  GMKLLRK ++  EK++ A +GFGPE S
Sbjct: 7   LLEWPKKDNRRFLHVVYRVGDLDRTIEFYTEVFGMKLLRKRDIPEEKYSNAFLGFGPETS 66

Query: 76  SFVLELTYEHGVTSYDIGTGFGHFAIATQDVGEVNFFMVYKMVENVRAKGGENMIIREPF 135
           +FV+ELTY +GV+SYDIGTGFGHFAI+TQDV         K+VENVRAKGG   + REP 
Sbjct: 67  NFVVELTYNYGVSSYDIGTGFGHFAISTQDVS--------KLVENVRAKGGN--VTREPG 126

Query: 136 ELKGSPNFLLAYVKDPNGYIFELIQRAQT-EPLCHLMLRVADLQRSIDFYQKALGMRVLT 195
            +KG  + ++A+VKDP+GY FELIQR  T EP C +MLRV DL R+I FY+KALGMR+L 
Sbjct: 127 PVKGGGS-VIAFVKDPDGYTFELIQRGPTPEPFCQVMLRVGDLDRAIKFYEKALGMRLLR 186

Query: 196 KVESVEQKYAIALMGYADELETTALELNYNHGVTQHSKGNGYSQVAIGTDDVYKSAEIVN 255
           K+E  E KY I +MGYA+E E+  LEL YN+ VT+++KGN Y+Q+AIGTDDVYKS E++ 
Sbjct: 187 KIERPEYKYTIGMMGYAEEYESIVLELTYNYDVTEYTKGNAYAQIAIGTDDVYKSGEVIK 246

Query: 256 LITKELGGKIIQPPSLDSQMNSKITSFLDPDGWKIVLVDNEDYLKGMQ 303
           ++ +ELGGKI +       + +KI SFLDPDGWK VLVDN+D+LK ++
Sbjct: 247 IVNQELGGKITREAGPLPGLGTKIVSFLDPDGWKTVLVDNKDFLKELE 283

The following BLAST results are available for this feature:

BLAST of Chy12G216700 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O65398	1.1e-98	61.11	Lactoylglutathione lyase GLX1 OS=Arabidopsis thaliana OX=3702 GN=GLX1 PE=1 SV=1	[more]
Q948T6	5.8e-97	59.66	Lactoylglutathione lyase OS=Oryza sativa subsp. japonica OX=39947 GN=GLYI-11 PE=...	[more]
Q39366	5.0e-96	60.76	Putative lactoylglutathione lyase OS=Brassica oleracea var. gemmifera OX=178616 ...	[more]
Q8W593	3.0e-85	52.81	Probable lactoylglutathione lyase, chloroplastic OS=Arabidopsis thaliana OX=3702...	[more]
P46235	4.1e-26	44.29	Probable lactoylglutathione lyase OS=Vibrio parahaemolyticus serotype O3:K6 (str...	[more]

BLAST of Chy12G216700 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LS05	3.0e-160	93.38	Glyoxalase I OS=Cucumis sativus OX=3659 GN=Csa_1G062880 PE=3 SV=1	[more]
A0A5D3BI05	3.3e-151	90.43	Glyoxalase I OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G005690 ...	[more]
E5GC58	3.3e-151	90.43	Glyoxalase I OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1	[more]
A0A1S3B2D8	3.3e-151	90.43	Glyoxalase I OS=Cucumis melo OX=3656 GN=LOC103485234 PE=3 SV=1	[more]
A0A6J1CEU9	5.3e-125	76.57	Lactoylglutathione lyase OS=Momordica charantia OX=3673 GN=LOC111010851 PE=3 SV=...	[more]

BLAST of Chy12G216700 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004145118.1	1.52e-204	93.38	lactoylglutathione lyase GLX1 [Cucumis sativus] >KGN64513.1 hypothetical protein...	[more]
XP_008440988.1	1.17e-192	90.43	PREDICTED: putative lactoylglutathione lyase [Cucumis melo] >ADN34057.1 lactoylg...	[more]
XP_038895603.1	3.95e-168	80.41	LOW QUALITY PROTEIN: lactoylglutathione lyase GLX1 [Benincasa hispida]	[more]
XP_022140116.1	3.01e-158	76.57	putative lactoylglutathione lyase [Momordica charantia]	[more]
XP_022994208.1	1.49e-146	71.76	LOW QUALITY PROTEIN: lactoylglutathione lyase GLX1 [Cucurbita maxima]	[more]

BLAST of Chy12G216700 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G11840.6	2.0e-100	59.67	glyoxalase I homolog	[more]
AT1G11840.2	7.6e-100	61.11	glyoxalase I homolog	[more]
AT1G11840.3	7.6e-100	61.11	glyoxalase I homolog	[more]
AT1G11840.1	7.6e-100	61.11	glyoxalase I homolog	[more]
AT1G11840.4	7.6e-100	61.11	glyoxalase I homolog	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004360	Glyoxalase/fosfomycin resistance/dioxygenase domain	PFAM	PF00903	Glyoxalase	coord: 29..158 e-value: 2.4E-12 score: 47.2 coord: 169..289 e-value: 7.5E-11 score: 42.3
IPR029068	Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase	GENE3D	3.10.180.10		coord: 164..301 e-value: 2.1E-35 score: 123.9
IPR029068	Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase	GENE3D	3.10.180.10		coord: 5..163 e-value: 2.9E-38 score: 133.2
IPR029068	Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase	SUPERFAMILY	54593	Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase	coord: 167..294
IPR029068	Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase	SUPERFAMILY	54593	Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase	coord: 26..162
IPR004361	Glyoxalase I	TIGRFAM	TIGR00068	TIGR00068	coord: 15..165 e-value: 9.2E-45 score: 149.7
None	No IPR available	PANTHER	PTHR46036:SF8	LACTOYLGLUTATHIONE LYASE-LIKE PROTEIN	coord: 14..301
None	No IPR available	PANTHER	PTHR46036	LACTOYLGLUTATHIONE LYASE	coord: 14..301
IPR018146	Glyoxalase I, conserved site	PROSITE	PS00935	GLYOXALASE_I_2	coord: 93..105
IPR037523	Vicinal oxygen chelate (VOC) domain	PROSITE	PS51819	VOC	coord: 166..294 score: 20.796955
IPR037523	Vicinal oxygen chelate (VOC) domain	PROSITE	PS51819	VOC	coord: 26..161 score: 18.326258

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy12G216700.1	Chy12G216700.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019243	methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0004462	lactoylglutathione lyase activity
molecular_function	GO:0046872	metal ion binding