Chy12G215710 (gene) Cucumber (hystrix) v1

Overview


Name	Chy12G215710
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	Aspergillus nuclease S(1)
Location	chrH12: 15011842 .. 15014320 (-)
RNA-Seq Expression	Chy12G215710
Synteny	Chy12G215710

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGAAAATTGGTATTTAGATTTTTGGTGGTGTTGAGTTTTATTTCATTCCTTCTAGTCCTGCCTTGTGCTCAAGGATGGAGCAAAGAAGGTCATATCCTAACATGTGAAATTGCACAGGTAACTTTATTTACTTTTTCTTGTCTGATGATCTTTTAATTAAATTGGACGTTTTGGATCAAATTCTACCTTGACCATTTGAACACAAATAGGGTCATTTTTACTCTTAAATTTGACAAACTGTCTCCATTTTTATCTGCAATTTTATATTTTCTTTAATCAGTTTTTGTATCAGCTTAAAAAAAATTGTTATATGGGTGGGATCTAAGGAGTTTGTTATGAAATCAACAAATAAACCAAGCAGAAAAACGTAAATGACGAGAGAATCGGCATAGAAGCATATTAGCTACATTTATGCTCATTAGAAAGAATATTTCAGATTACAGAACAAAGACGCTTCTATAAATGGGGAGTTCATACAACCAATTTGATCTTCAAATCTTAAGGTTAAAATGGTAAACAACGTAAATACAGTGAGATCTTAAACATTTTTGTTTAATTAGTTGTTGAGAGGTGAGATATCAGATCTAAATGTATTCAACTCAGTTATAAAATATATATCAGGCAAGAAACTGATGTGTTTTGAAAGAGAAATTAACACCAAAACAGATTTTGGGCTGAATTCTGGAAGATTTATCAGACAATTGACATTGTACATTGATCTAGCTCAATTCGATCATGTCAAACTTGATTTTTTGTTGAAAGCAGTGAGAGTCTTAAAAAGGAGGATTTTACATGTTAAACAAATTTAGAAACAAAAATGTAAAATTTGACACACATAATTGAATATTCAAGAGGACAACTAATGGGTAACAATGTTTTTCCCTAATTAATCCAACCACATTCAAGGAGCTACTAATTCCAGAGGCAGCAGAGGCAGTTCAAGATCTGTTACCTGAAAGTGCCGGAGGAAATTTATCGGCGATGTGCATATGGCCAGACCAAATCCGACTCCAGTCAAAGTACCGCTGGGCCAGTCCCCTTCACTACGCCAACACCCCCGACTCTTGTTCTTTCGTCTACAAAAGTAAAGTAACACCTTTTTATTCTCCCATCCCTAAGGTTAAATAAATTCATTTCGAATTATTAAACTAACCGGTGCGTTGTTGTTTAGGGGATTGTCATAATGACGCCGGACAGCCGGACATGTGCGTCGCCGGTGCCATTCGAAATTTCACCACTCAGCTCACAACCTACAGAACACAAGGCTTCGACAGTCCTCGTAAATGATCCGACCCGACCCGACCCACTCGAATTATTCGAGTGATAATATGAAATTTGTTTAATTTCATTTTTATTATTGTAGATAACTTGACCGAGGCGTTACTCTTTCTATCGCATTTCGTTGGGGATATTCATCAGGTGATACTAAAGTTATTCAAAAACGTTTATTTGTTGAATTGCTTTAATTTTATTATGAGATATGTAATTATAACTTATTTTACATATATATAATTTCAGCCATTACATGTGGGGTTCGAGAGTGATGCGGGAGGAAACACCATAGAAGTACGGTGGTTCCGTCGTAAATCAAATCTCCATCACGTAAGATCTTAATCTAAAAACGTATCAAAATAATAGATGTGTTTTAAGTTTATTATGAGTTCTATTAGATTTAAATTATAAATTAATAATTATATGGATTAATGCAGTTATTGTTATTATTATTATTAAAAAGATTAAAGCTTAGGATTAATTCTTGCCTTATAATTTAACCAAAGAAAAAAAAAATGATCCTATGCAGGTGTGGGATAGGGACATTATTCTTGAAGCTCTTGGAGATTATTACGACAAAGACGGTGGCCTCCTCTTAGATGAACTTGACAGGAATTTGACTGCGGTAATTAATTAATACTCCTATTTTTCCTTTTATTAGGTTAATACATTAATTTATAACATTAGGATATTACAAAATTTATTATGTTCTTGTCAAATTTCCATTTTTGTAGGGAATTTGGAGCAATGACGTTCCGGTATGGGAGCGTTGTCCTACAATTAATTCATGTGTTAATAAGTATGTATAGAATATGAAATTAGGTTATATTTGGTAAATATTTTTTGAACAATAATACTAACAACTTGTGGATTAGGTGGGCTGATGAGAGTACGGCCTTGGCTTGCAAGTGGGCATATGAAGGAGTTGAAGCTGGTATTACTTTATCAGGTAAACCCAATCAATCAATCAAACATTGTTTAAATTAATATCATAGTCATTGATTCTTAAAATGATTTGAAATATAATATATGATCAAAGGATAATGAAATCCGGAATTGATGTGATTTGTGTACAGAGGAATACTACGATTCAAGGTTGCCAATTGTGATGGAACGATTAGCTCAAGGTGGGGTCCGGTTGGCAATGCTCTTGAACCGGGTTTTTGCTGAAGATGCTACACGAGGATTTGCCTATTCATCTTGA

mRNA sequence

ATGGGAAAATTGGTATTTAGATTTTTGGTGGTGTTGAGTTTTATTTCATTCCTTCTAGTCCTGCCTTGTGCTCAAGGATGGAGCAAAGAAGGTCATATCCTAACATGTGAAATTGCACAGGAGCTACTAATTCCAGAGGCAGCAGAGGCAGTTCAAGATCTGTTACCTGAAAGTGCCGGAGGAAATTTATCGGCGATGTGCATATGGCCAGACCAAATCCGACTCCAGTCAAAGTACCGCTGGGCCAGTCCCCTTCACTACGCCAACACCCCCGACTCTTGTTCTTTCGTCTACAAAAGGGATTGTCATAATGACGCCGGACAGCCGGACATGTGCGTCGCCGGTGCCATTCGAAATTTCACCACTCAGCTCACAACCTACAGAACACAAGGCTTCGACAGTCCTCATAACTTGACCGAGGCGTTACTCTTTCTATCGCATTTCGTTGGGGATATTCATCAGCCATTACATGTGGGGTTCGAGAGTGATGCGGGAGGAAACACCATAGAAGTACGGTGGTTCCGTCGTAAATCAAATCTCCATCACGTGTGGGATAGGGACATTATTCTTGAAGCTCTTGGAGATTATTACGACAAAGACGGTGGCCTCCTCTTAGATGAACTTGACAGGAATTTGACTGCGGGAATTTGGAGCAATGACGTTCCGGTATGGGAGCGTTGTCCTACAATTAATTCATGTGTTAATAAGTGGGCTGATGAGAGTACGGCCTTGGCTTGCAAGTGGGCATATGAAGGAGTTGAAGCTGGTATTACTTTATCAGAGGAATACTACGATTCAAGGTTGCCAATTGTGATGGAACGATTAGCTCAAGGTGGGGTCCGGTTGGCAATGCTCTTGAACCGGGTTTTTGCTGAAGATGCTACACGAGGATTTGCCTATTCATCTTGA

Coding sequence (CDS)

ATGGGAAAATTGGTATTTAGATTTTTGGTGGTGTTGAGTTTTATTTCATTCCTTCTAGTCCTGCCTTGTGCTCAAGGATGGAGCAAAGAAGGTCATATCCTAACATGTGAAATTGCACAGGAGCTACTAATTCCAGAGGCAGCAGAGGCAGTTCAAGATCTGTTACCTGAAAGTGCCGGAGGAAATTTATCGGCGATGTGCATATGGCCAGACCAAATCCGACTCCAGTCAAAGTACCGCTGGGCCAGTCCCCTTCACTACGCCAACACCCCCGACTCTTGTTCTTTCGTCTACAAAAGGGATTGTCATAATGACGCCGGACAGCCGGACATGTGCGTCGCCGGTGCCATTCGAAATTTCACCACTCAGCTCACAACCTACAGAACACAAGGCTTCGACAGTCCTCATAACTTGACCGAGGCGTTACTCTTTCTATCGCATTTCGTTGGGGATATTCATCAGCCATTACATGTGGGGTTCGAGAGTGATGCGGGAGGAAACACCATAGAAGTACGGTGGTTCCGTCGTAAATCAAATCTCCATCACGTGTGGGATAGGGACATTATTCTTGAAGCTCTTGGAGATTATTACGACAAAGACGGTGGCCTCCTCTTAGATGAACTTGACAGGAATTTGACTGCGGGAATTTGGAGCAATGACGTTCCGGTATGGGAGCGTTGTCCTACAATTAATTCATGTGTTAATAAGTGGGCTGATGAGAGTACGGCCTTGGCTTGCAAGTGGGCATATGAAGGAGTTGAAGCTGGTATTACTTTATCAGAGGAATACTACGATTCAAGGTTGCCAATTGTGATGGAACGATTAGCTCAAGGTGGGGTCCGGTTGGCAATGCTCTTGAACCGGGTTTTTGCTGAAGATGCTACACGAGGATTTGCCTATTCATCTTGA

Protein sequence

MGKLVFRFLVVLSFISFLLVLPCAQGWSKEGHILTCEIAQELLIPEAAEAVQDLLPESAGGNLSAMCIWPDQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLTTYRTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHVWDRDIILEALGDYYDKDGGLLLDELDRNLTAGIWSNDVPVWERCPTINSCVNKWADESTALACKWAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFAEDATRGFAYSS*

Homology

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BLAST of Chy12G215710 vs. ExPASy Swiss-Prot
Match: Q9SXA6 (Endonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=ENDO1 PE=1 SV=1)

HSP 1 Score: 362.8 bits (930), Expect = 3.7e-99
Identity = 166/286 (58.04%), Postives = 217/286 (75.87%), Query Frame = 0


Query: 9   LVVLSFISFLLVLPCAQGWSKEGHILTCEIAQELLIPEAAEAVQDLLPESAGGNLSAMCI 68
           L+++  I  L  +   + WSKEGHILTC IAQ LL    A  V++LLP+   G+LSA+C+
Sbjct: 11  LILVLGILILCSVSSVRSWSKEGHILTCRIAQNLLEAGPAHVVENLLPDYVKGDLSALCV 70

Query: 69  WPDQIRLQSKYRWASPLHYANTPD-SCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLTTY 128
           WPDQIR   KYRW S LHY +TPD +CS+ Y RDCH+  G  DMCV GAI+NFT+QL  Y
Sbjct: 71  WPDQIRHWYKYRWTSHLHYIDTPDQACSYEYSRDCHDQHGLKDMCVDGAIQNFTSQLQHY 130

Query: 129 RTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHVWDRD 188
                D  +N+TEALLFLSHF+GDIHQP+HVGF SD GGNTI++RW++ KSNLHHVWDR+
Sbjct: 131 GEGTSDRRYNMTEALLFLSHFMGDIHQPMHVGFTSDEGGNTIDLRWYKHKSNLHHVWDRE 190

Query: 189 IILEALGDYYDKDGGLLLDELDRNLTAGIWSNDVPVWERCPTINSCVNKWADESTALACK 248
           IIL AL + YDK+  LL ++L++N+T G+W +D+  W  C  + +C +K+A ES  LACK
Sbjct: 191 IILTALKENYDKNLDLLQEDLEKNITNGLWHDDLSSWTECNDLIACPHKYASESIKLACK 250

Query: 249 WAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFAED 294
           W Y+GV++G TLSEEY+++RLPIVM+R+ QGGVRLAM+LNRVF++D
Sbjct: 251 WGYKGVKSGETLSEEYFNTRLPIVMKRIVQGGVRLAMILNRVFSDD 296

[Close]

BLAST of Chy12G215710 vs. ExPASy Swiss-Prot
Match: Q9C9G4 (Endonuclease 2 OS=Arabidopsis thaliana OX=3702 GN=ENDO2 PE=1 SV=1)

HSP 1 Score: 315.8 bits (808), Expect = 5.1e-85
Identity = 148/283 (52.30%), Postives = 199/283 (70.32%), Query Frame = 0


Query: 9   LVVLSFISFLLVLPCAQGWSKEGHILTCEIAQELLIPEAAEAVQDLLPESAGGNLSAMCI 68
           ++++  +  L   P   GW KEGH + C+IAQ  L   AA+AV++LLPESA G+LS++C+
Sbjct: 10  VMMIITVWLLYAAPNIHGWGKEGHEIICKIAQTRLDETAAKAVKELLPESAEGDLSSLCL 69

Query: 69  WPDQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLTTYR 128
           W D+++   +Y W+SPLHY NTPD+CS+ Y RDC +++G+   CVAGAI N+TTQL +Y+
Sbjct: 70  WADRVKF--RYHWSSPLHYINTPDACSYQYNRDCKDESGEKGRCVAGAIYNYTTQLLSYK 129

Query: 129 T-QGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHVWDRD 188
           T     S +NLTEALLF+SHF+GDIHQPLHV + SD GGNTIEV W+ RK+NLHH+WD +
Sbjct: 130 TAASSQSQYNLTEALLFVSHFMGDIHQPLHVSYASDKGGNTIEVHWYTRKANLHHIWDSN 189

Query: 189 IILEALGDYYDKDGGLLLDELDRNLTAGIWSNDVPVWERCPTINSCVNKWADESTALACK 248
           II  A  D Y+     ++D L +N+T   W++ V  WE C    +C + +A E    AC 
Sbjct: 190 IIETAEADLYNSALEGMVDALKKNITTE-WADQVKRWETCTKKTACPDIYASEGIQAACD 249

Query: 249 WAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVF 291
           WAY+GV  G TL +EY+ SRLPIV +RLAQGGVRLA  LNR+F
Sbjct: 250 WAYKGVTEGDTLEDEYFYSRLPIVYQRLAQGGVRLAATLNRIF 289

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BLAST of Chy12G215710 vs. ExPASy Swiss-Prot
Match: F4JJL0 (Endonuclease 4 OS=Arabidopsis thaliana OX=3702 GN=ENDO4 PE=1 SV=1)

HSP 1 Score: 303.5 bits (776), Expect = 2.6e-81
Identity = 146/268 (54.48%), Postives = 183/268 (68.28%), Query Frame = 0


Query: 27  WSKEGHILTCEIAQELLIPEAAEAVQDLLPESAGGNLSAMCIWPDQIRLQSKYRWASPLH 86
           W KEGH   C+IA+     E   AV+ LLP+SA G+L+++C WPD+I+   ++RW SPLH
Sbjct: 25  WGKEGHYTVCKIAESYFEEETVAAVKKLLPKSADGDLASVCSWPDEIKHHWQWRWTSPLH 84

Query: 87  YANTPD-SCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLTTYRTQGFDSPH-NLTEALLF 146
           Y +TPD  C++ Y RDCH+     D CV GAI N+T QL +         H NLTEAL+F
Sbjct: 85  YVDTPDYRCNYEYCRDCHDTHKNQDRCVTGAIFNYTMQLMSASENSDTIVHYNLTEALMF 144

Query: 147 LSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHVWDRDIILEALGDYYDKDGGLL 206
           LSHF+GDIHQPLHVGF  D GGNTI VRW+RRK+NLHHVWD  II  AL  YY+K   L+
Sbjct: 145 LSHFIGDIHQPLHVGFLGDEGGNTITVRWYRRKTNLHHVWDNMIIESALKTYYNKSLPLM 204

Query: 207 LDELDRNLTAGIWSNDVPVWERCP-TINSCVNKWADESTALACKWAYEGVEAGITLSEEY 266
           ++ L  NLT   WSNDVP+WE C     +C N +A ES  LACK+AY     G TL ++Y
Sbjct: 205 IEALQANLT-NDWSNDVPLWESCQLNQTACPNPYASESINLACKYAYRNATPGTTLGDDY 264

Query: 267 YDSRLPIVMERLAQGGVRLAMLLNRVFA 292
           + SRLPIV +RLAQGG+RLA  LNR+F+
Sbjct: 265 FLSRLPIVEKRLAQGGIRLAATLNRIFS 291

[Close]

BLAST of Chy12G215710 vs. ExPASy Swiss-Prot
Match: F4JJL3 (Endonuclease 5 OS=Arabidopsis thaliana OX=3702 GN=ENDO5 PE=1 SV=1)

HSP 1 Score: 275.0 bits (702), Expect = 1.0e-72
Identity = 139/287 (48.43%), Postives = 179/287 (62.37%), Query Frame = 0


Query: 12  LSFISFLLVLPCAQG---WSKEGHILTCEIAQELLIPEAAEAVQDLLPESA-GGNLSAMC 71
           L  +S L++     G   W K+GH   C++A+     +   AV+ LLPES  GG L+  C
Sbjct: 3   LWIVSVLVLTHLVHGALCWGKDGHYTVCKLAEGFFEDDTIAAVKKLLPESVDGGGLADFC 62

Query: 72  IWPDQIRLQSKYRWASPLHYANTPD-SCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLTT 131
            WPD+I+  S+++W S LHY NTP+  C++ Y RDCH+     D CV GAI N+T QL +
Sbjct: 63  SWPDEIKKLSQWQWTSTLHYVNTPEYRCNYEYCRDCHDTHKHKDWCVTGAIFNYTNQLMS 122

Query: 132 YRTQGFDSPH-NLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHVWD 191
                 +  H NLTEALLFLSH++GD+HQPLH GF  D GGNTI V W+  KSNLHHVWD
Sbjct: 123 ASENSQNIVHYNLTEALLFLSHYMGDVHQPLHTGFLGDLGGNTIIVNWYHNKSNLHHVWD 182

Query: 192 RDIILEALGDYYDKDGGLLLDELDRNLTAGIWSNDVPVWERCP-TINSCVNKWADESTAL 251
             II  AL  YY+     ++  L   L  G WSNDVP W+ C     +C N +A ES  L
Sbjct: 183 NMIIDSALETYYNSSLPHMIQALQAKLKNG-WSNDVPSWKSCHFHQKACPNLYASESIDL 242

Query: 252 ACKWAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFA 292
           ACK+AY     G TL +EY+ SRLP+V +RLAQGG+RLA  LNR+F+
Sbjct: 243 ACKYAYRNATPGTTLGDEYFLSRLPVVEKRLAQGGIRLAATLNRIFS 288

[Close]

BLAST of Chy12G215710 vs. ExPASy Swiss-Prot
Match: Q8LDW6 (Endonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=ENDO3 PE=1 SV=1)

HSP 1 Score: 270.0 bits (689), Expect = 3.2e-71
Identity = 137/283 (48.41%), Postives = 176/283 (62.19%), Query Frame = 0


Query: 15  ISFLLVLPCAQG---WSKEGHILTCEIAQELLIPEAAEAVQDLLPESAGGNLSAMCIWPD 74
           +S L++     G   W   GH   C+IAQ     +   AV+ LLPESA G L+A+C WPD
Sbjct: 10  VSILVLTQLVNGALCWGDAGHYAVCKIAQSYFEEDTVVAVKKLLPESANGELAAVCSWPD 69

Query: 75  QIRLQSKYRWASPLHYANTPD-SCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLTTYRTQ 134
           +I+   ++RW S LH+A+TPD  C++ Y RDC       D CV GAI N+T QL +    
Sbjct: 70  EIKKLPQWRWTSALHFADTPDYKCNYEYSRDC-----PKDWCVTGAIFNYTNQLMSTSEN 129

Query: 135 GFDSPH-NLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHVWDRDII 194
                H NLTEAL+FLSH++GDIHQPLH GF  D GGN I+V W+ +++NLH VWD  II
Sbjct: 130 SQSIVHYNLTEALMFLSHYMGDIHQPLHEGFIGDLGGNKIKVHWYNQETNLHRVWDDMII 189

Query: 195 LEALGDYYDKDGGLLLDELDRNLTAGIWSNDVPVWERCP-TINSCVNKWADESTALACKW 254
             AL  YY+     ++ EL   L  G WSNDVP WE C     +C N +A ES  LACK+
Sbjct: 190 ESALETYYNSSLPRMIHELQAKLKNG-WSNDVPSWESCQLNQTACPNPYASESIDLACKY 249

Query: 255 AYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFA 292
           AY    AG TL + Y+ SRLP+V +RLAQGG+RLA  LNR+F+
Sbjct: 250 AYRNATAGTTLGDYYFVSRLPVVEKRLAQGGIRLAGTLNRIFS 286

[Close]

BLAST of Chy12G215710 vs. ExPASy TrEMBL
Match: C3VEY2 (Aspergillus nuclease S(1) OS=Cucumis sativus OX=3659 PE=2 SV=2)

HSP 1 Score: 609.0 bits (1569), Expect = 1.1e-170
Identity = 290/302 (96.03%), Postives = 294/302 (97.35%), Query Frame = 0


Query: 1   MGKLVFRFLVVLSFISFLLVLPCAQGWSKEGHILTCEIAQELLIPEAAEAVQDLLPESAG 60
           MGKLV  FLVVL FISFLLVLPCAQGWSKEGHILTCEIAQELLIPEAAEAVQDLLPESAG
Sbjct: 1   MGKLV--FLVVLIFISFLLVLPCAQGWSKEGHILTCEIAQELLIPEAAEAVQDLLPESAG 60

Query: 61  GNLSAMCIWPDQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNF 120
           GNLSAMC+WPDQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNF
Sbjct: 61  GNLSAMCVWPDQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNF 120

Query: 121 TTQLTTYRTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNL 180
           TTQLTTYRTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNL
Sbjct: 121 TTQLTTYRTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNL 180

Query: 181 HHVWDRDIILEALGDYYDKDGGLLLDELDRNLTAGIWSNDVPVWERCPTINSCVNKWADE 240
           HHVWDRDIILEALGDYYDKDGGLLLDEL+RNLT GIWSNDV  WERC T+NSCVN+WADE
Sbjct: 181 HHVWDRDIILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCSTVNSCVNRWADE 240

Query: 241 STALACKWAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFAEDATRGFAY 300
           ST LACKWAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFAEDATRGFAY
Sbjct: 241 STGLACKWAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFAEDATRGFAY 300

Query: 301 SS 303
           SS
Sbjct: 301 SS 300

[Close]

BLAST of Chy12G215710 vs. ExPASy TrEMBL
Match: A0A5D3DSP7 (Aspergillus nuclease S(1) OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861G00370 PE=3 SV=1)

HSP 1 Score: 592.8 bits (1527), Expect = 8.0e-166
Identity = 279/302 (92.38%), Postives = 288/302 (95.36%), Query Frame = 0


Query: 1   MGKLVFRFLVVLSFISFLLVLPCAQGWSKEGHILTCEIAQELLIPEAAEAVQDLLPESAG 60
           MGKL FRFLVVLSFISFLLVLPCAQGWSKEGHILTCEIAQELL PEAA+AVQDLLPESAG
Sbjct: 1   MGKLAFRFLVVLSFISFLLVLPCAQGWSKEGHILTCEIAQELLNPEAADAVQDLLPESAG 60

Query: 61  GNLSAMCIWPDQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNF 120
           GNLSAMC+W DQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNF
Sbjct: 61  GNLSAMCVWADQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNF 120

Query: 121 TTQLTTYRTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNL 180
           TTQLTTYRTQG DSPHNLTEALLFLSHFVGDIHQPLHVGF SD GGNTIEVRWFRRKSNL
Sbjct: 121 TTQLTTYRTQGSDSPHNLTEALLFLSHFVGDIHQPLHVGFASDEGGNTIEVRWFRRKSNL 180

Query: 181 HHVWDRDIILEALGDYYDKDGGLLLDELDRNLTAGIWSNDVPVWERCPTINSCVNKWADE 240
           HHVWDRDIIL AL DYYDKDGGLLL+EL RNLT GIWSNDVP WERC  +NSCVNKWA+E
Sbjct: 181 HHVWDRDIILTALADYYDKDGGLLLEELQRNLTQGIWSNDVPTWERCVKVNSCVNKWAEE 240

Query: 241 STALACKWAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFAEDATRGFAY 300
           ST LACKWAYEGVEAGITLSE+Y+DSRLPIVMERLAQGGVRLAMLLNRVF+EDAT+GFAY
Sbjct: 241 STDLACKWAYEGVEAGITLSEDYFDSRLPIVMERLAQGGVRLAMLLNRVFSEDATQGFAY 300

Query: 301 SS 303
           SS
Sbjct: 301 SS 302

[Close]

BLAST of Chy12G215710 vs. ExPASy TrEMBL
Match: A0A1S3B4B0 (Aspergillus nuclease S(1) OS=Cucumis melo OX=3656 GN=LOC103486022 PE=3 SV=1)

HSP 1 Score: 592.8 bits (1527), Expect = 8.0e-166
Identity = 279/302 (92.38%), Postives = 288/302 (95.36%), Query Frame = 0


Query: 1   MGKLVFRFLVVLSFISFLLVLPCAQGWSKEGHILTCEIAQELLIPEAAEAVQDLLPESAG 60
           MGKL FRFLVVLSFISFLLVLPCAQGWSKEGHILTCEIAQELL PEAA+AVQDLLPESAG
Sbjct: 1   MGKLAFRFLVVLSFISFLLVLPCAQGWSKEGHILTCEIAQELLNPEAADAVQDLLPESAG 60

Query: 61  GNLSAMCIWPDQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNF 120
           GNLSAMC+W DQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNF
Sbjct: 61  GNLSAMCVWADQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNF 120

Query: 121 TTQLTTYRTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNL 180
           TTQLTTYRTQG DSPHNLTEALLFLSHFVGDIHQPLHVGF SD GGNTIEVRWFRRKSNL
Sbjct: 121 TTQLTTYRTQGSDSPHNLTEALLFLSHFVGDIHQPLHVGFASDEGGNTIEVRWFRRKSNL 180

Query: 181 HHVWDRDIILEALGDYYDKDGGLLLDELDRNLTAGIWSNDVPVWERCPTINSCVNKWADE 240
           HHVWDRDIIL AL DYYDKDGGLLL+EL RNLT GIWSNDVP WERC  +NSCVNKWA+E
Sbjct: 181 HHVWDRDIILTALADYYDKDGGLLLEELQRNLTQGIWSNDVPTWERCVKVNSCVNKWAEE 240

Query: 241 STALACKWAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFAEDATRGFAY 300
           ST LACKWAYEGVEAGITLSE+Y+DSRLPIVMERLAQGGVRLAMLLNRVF+EDAT+GFAY
Sbjct: 241 STDLACKWAYEGVEAGITLSEDYFDSRLPIVMERLAQGGVRLAMLLNRVFSEDATQGFAY 300

Query: 301 SS 303
           SS
Sbjct: 301 SS 302

[Close]

BLAST of Chy12G215710 vs. ExPASy TrEMBL
Match: A0A0A0LUG7 (Aspergillus nuclease S(1) OS=Cucumis sativus OX=3659 GN=Csa_1G072450 PE=3 SV=1)

HSP 1 Score: 496.5 bits (1277), Expect = 7.8e-137
Identity = 228/237 (96.20%), Postives = 232/237 (97.89%), Query Frame = 0


Query: 66  MCIWPDQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLT 125
           MC+WPDQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLT
Sbjct: 1   MCVWPDQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLT 60

Query: 126 TYRTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHVWD 185
           TYRTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHVWD
Sbjct: 61  TYRTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHVWD 120

Query: 186 RDIILEALGDYYDKDGGLLLDELDRNLTAGIWSNDVPVWERCPTINSCVNKWADESTALA 245
           RDIILEALGDYYDKDGGLLLDEL+RNLT GIWSNDV  WERC T+NSCVN+WADEST LA
Sbjct: 121 RDIILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCSTVNSCVNRWADESTGLA 180

Query: 246 CKWAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFAEDATRGFAYSS 303
           CKWAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFAEDATRGFAYSS
Sbjct: 181 CKWAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFAEDATRGFAYSS 237

[Close]

BLAST of Chy12G215710 vs. ExPASy TrEMBL
Match: A0A6J1ENU3 (Aspergillus nuclease S(1) OS=Cucurbita moschata OX=3662 GN=LOC111434241 PE=3 SV=1)

HSP 1 Score: 481.1 bits (1237), Expect = 3.4e-132
Identity = 228/298 (76.51%), Postives = 254/298 (85.23%), Query Frame = 0


Query: 5   VFRFLVVLSFISFLLVLPCAQGWSKEGHILTCEIAQELLIPEAAEAVQDLLPESAGGNLS 64
           VFRFLV+L F    L+LP AQGWSKEGH+LTC+IAQELL PEA EAVQ LLPESAGGNLS
Sbjct: 3   VFRFLVLLCFT--FLLLPSAQGWSKEGHVLTCQIAQELLNPEATEAVQALLPESAGGNLS 62

Query: 65  AMCIWPDQIRLQSKYRWASPLHYANTPD-SCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQ 124
           AMC+W DQIR  SKYRW SPLHY NTPD +CSF+YKRDCHN A Q +MCVAGAIRNFTTQ
Sbjct: 63  AMCVWADQIRRWSKYRWTSPLHYINTPDNACSFLYKRDCHNTAAQVNMCVAGAIRNFTTQ 122

Query: 125 LTTYRTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHV 184
           LT +  QG D+ +NLTEALLFLSHFVGDIHQPLHVGF SD GGNTIE+RW+R KSNLHHV
Sbjct: 123 LTAFPKQGPDAKNNLTEALLFLSHFVGDIHQPLHVGFTSDEGGNTIELRWYRHKSNLHHV 182

Query: 185 WDRDIILEALGDYYDKDGGLLLDELDRNLTAGIWSNDVPVWERCPTINSCVNKWADESTA 244
           WDR+IIL AL DYYDKD  LLL++L RNLT GIWS+DVP WERC  +NSC+N WA+ES  
Sbjct: 183 WDREIILTALADYYDKDTDLLLEDLQRNLTHGIWSDDVPTWERCVNVNSCINNWAEESIK 242

Query: 245 LACKWAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFAEDATRGFAYS 302
           LAC WAYEGVEAG+TLSE+Y+DSRLPIVMERLA+GGVRLAMLLNRVF+E+   GF  S
Sbjct: 243 LACTWAYEGVEAGMTLSEDYFDSRLPIVMERLAKGGVRLAMLLNRVFSENPKGGFRSS 298

[Close]

BLAST of Chy12G215710 vs. NCBI nr
Match: NP_001292654.1 (endonuclease 1 precursor [Cucumis sativus] >ACO72982.2 bifunctional nuclease precursor [Cucumis sativus] >KAE8652702.1 hypothetical protein Csa_013303 [Cucumis sativus])

HSP 1 Score: 606 bits (1563), Expect = 8.89e-219
Identity = 290/302 (96.03%), Postives = 294/302 (97.35%), Query Frame = 0


Query: 1   MGKLVFRFLVVLSFISFLLVLPCAQGWSKEGHILTCEIAQELLIPEAAEAVQDLLPESAG 60
           MGKLVF  LVVL FISFLLVLPCAQGWSKEGHILTCEIAQELLIPEAAEAVQDLLPESAG
Sbjct: 1   MGKLVF--LVVLIFISFLLVLPCAQGWSKEGHILTCEIAQELLIPEAAEAVQDLLPESAG 60

Query: 61  GNLSAMCIWPDQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNF 120
           GNLSAMC+WPDQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNF
Sbjct: 61  GNLSAMCVWPDQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNF 120

Query: 121 TTQLTTYRTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNL 180
           TTQLTTYRTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNL
Sbjct: 121 TTQLTTYRTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNL 180

Query: 181 HHVWDRDIILEALGDYYDKDGGLLLDELDRNLTAGIWSNDVPVWERCPTINSCVNKWADE 240
           HHVWDRDIILEALGDYYDKDGGLLLDEL+RNLT GIWSNDV  WERC T+NSCVN+WADE
Sbjct: 181 HHVWDRDIILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCSTVNSCVNRWADE 240

Query: 241 STALACKWAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFAEDATRGFAY 300
           ST LACKWAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFAEDATRGFAY
Sbjct: 241 STGLACKWAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFAEDATRGFAY 300

Query: 301 SS 302
           SS
Sbjct: 301 SS 300

[Close]

BLAST of Chy12G215710 vs. NCBI nr
Match: XP_008442043.1 (PREDICTED: endonuclease 1 [Cucumis melo] >KAA0056948.1 endonuclease 1 [Cucumis melo var. makuwa] >TYK26375.1 endonuclease 1 [Cucumis melo var. makuwa])

HSP 1 Score: 590 bits (1521), Expect = 2.42e-212
Identity = 279/302 (92.38%), Postives = 288/302 (95.36%), Query Frame = 0


Query: 1   MGKLVFRFLVVLSFISFLLVLPCAQGWSKEGHILTCEIAQELLIPEAAEAVQDLLPESAG 60
           MGKL FRFLVVLSFISFLLVLPCAQGWSKEGHILTCEIAQELL PEAA+AVQDLLPESAG
Sbjct: 1   MGKLAFRFLVVLSFISFLLVLPCAQGWSKEGHILTCEIAQELLNPEAADAVQDLLPESAG 60

Query: 61  GNLSAMCIWPDQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNF 120
           GNLSAMC+W DQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNF
Sbjct: 61  GNLSAMCVWADQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNF 120

Query: 121 TTQLTTYRTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNL 180
           TTQLTTYRTQG DSPHNLTEALLFLSHFVGDIHQPLHVGF SD GGNTIEVRWFRRKSNL
Sbjct: 121 TTQLTTYRTQGSDSPHNLTEALLFLSHFVGDIHQPLHVGFASDEGGNTIEVRWFRRKSNL 180

Query: 181 HHVWDRDIILEALGDYYDKDGGLLLDELDRNLTAGIWSNDVPVWERCPTINSCVNKWADE 240
           HHVWDRDIIL AL DYYDKDGGLLL+EL RNLT GIWSNDVP WERC  +NSCVNKWA+E
Sbjct: 181 HHVWDRDIILTALADYYDKDGGLLLEELQRNLTQGIWSNDVPTWERCVKVNSCVNKWAEE 240

Query: 241 STALACKWAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFAEDATRGFAY 300
           ST LACKWAYEGVEAGITLSE+Y+DSRLPIVMERLAQGGVRLAMLLNRVF+EDAT+GFAY
Sbjct: 241 STDLACKWAYEGVEAGITLSEDYFDSRLPIVMERLAQGGVRLAMLLNRVFSEDATQGFAY 300

Query: 301 SS 302
           SS
Sbjct: 301 SS 302

[Close]

BLAST of Chy12G215710 vs. NCBI nr
Match: XP_038895329.1 (LOW QUALITY PROTEIN: endonuclease 1-like [Benincasa hispida])

HSP 1 Score: 513 bits (1322), Expect = 6.39e-182
Identity = 247/302 (81.79%), Postives = 265/302 (87.75%), Query Frame = 0


Query: 6   FRFLVVLSFISFLLVLPCAQGWSKEGHILTCEIAQELLIPEAAEAVQDLLPESAGGNLSA 65
           FRFLV+LS ISFL VLPCA GWSKEGH+LTC+IAQELL  EA EAVQDLLPESAGGNLSA
Sbjct: 8   FRFLVLLSSISFL-VLPCAHGWSKEGHVLTCQIAQELLNGEATEAVQDLLPESAGGNLSA 67

Query: 66  MCIWPDQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLT 125
           +C+W DQIRLQSKYRWASPLHYANTPD+CSF+YKRDCHN AGQPDMCVAGAIRNFTTQLT
Sbjct: 68  LCVWADQIRLQSKYRWASPLHYANTPDTCSFLYKRDCHNTAGQPDMCVAGAIRNFTTQLT 127

Query: 126 TYRTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWF-----RRKSNL 185
           TYRTQG DSPHNLTEALLFLSHFVGDIHQPLHVGFESD GGNTIE   F     R++   
Sbjct: 128 TYRTQGSDSPHNLTEALLFLSHFVGDIHQPLHVGFESDEGGNTIESESFEIFNKRKRKXT 187

Query: 186 HHVWDRDIILEALGDYYDKDGGLLLDELDRNLTAGIWSNDVPVWERCPTINSCVNKWADE 245
             VWDRDIIL A+ DYYDKD GLLL+EL RNLT GIWSNDVP WE C  +NSCVNKWA+E
Sbjct: 188 TQVWDRDIILTAVADYYDKDTGLLLEELQRNLTNGIWSNDVPAWESCVKVNSCVNKWAEE 247

Query: 246 STALACKWAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFAEDATRGFAY 302
           S  LACKWAYEGVEAG+TLS++Y+DSRLPIV ERLAQGGVRLAMLLNRVF+ED TRGFA 
Sbjct: 248 SIDLACKWAYEGVEAGMTLSDDYFDSRLPIVTERLAQGGVRLAMLLNRVFSEDTTRGFAS 307

[Close]

BLAST of Chy12G215710 vs. NCBI nr
Match: KAG6584021.1 (Endonuclease 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019639.1 Endonuclease 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 479 bits (1234), Expect = 1.17e-168
Identity = 230/298 (77.18%), Postives = 256/298 (85.91%), Query Frame = 0


Query: 5   VFRFLVVLSFISFLLVLPCAQGWSKEGHILTCEIAQELLIPEAAEAVQDLLPESAGGNLS 64
           VFRFLV+L F +FLL LP AQGWSKEGH+LTC+IAQELL PEA EAVQ LLPESAGGNLS
Sbjct: 3   VFRFLVLLCF-TFLL-LPSAQGWSKEGHVLTCQIAQELLNPEATEAVQALLPESAGGNLS 62

Query: 65  AMCIWPDQIRLQSKYRWASPLHYANTPDS-CSFVYKRDCHNDAGQPDMCVAGAIRNFTTQ 124
           AMC+W DQIR  SKYRW SPLHY NTPD+ CSF+YKRDCHN A Q +MCVAGAIRNFTTQ
Sbjct: 63  AMCVWADQIRRSSKYRWTSPLHYINTPDNACSFLYKRDCHNTAAQLNMCVAGAIRNFTTQ 122

Query: 125 LTTYRTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHV 184
           LT +  QG D+ +NLTEALLFLSHFVGDIHQPLHVGF SD GGNTIE+RW+R KSNLHHV
Sbjct: 123 LTAFPKQGPDAKNNLTEALLFLSHFVGDIHQPLHVGFTSDEGGNTIELRWYRHKSNLHHV 182

Query: 185 WDRDIILEALGDYYDKDGGLLLDELDRNLTAGIWSNDVPVWERCPTINSCVNKWADESTA 244
           WDR+IIL AL DYYDKD  LLL++L RNLT GIWS+DVP WERC  +NSC+N WA+ES  
Sbjct: 183 WDREIILTALADYYDKDTDLLLEDLQRNLTHGIWSDDVPTWERCVNVNSCINNWAEESIK 242

Query: 245 LACKWAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFAEDATRGFAYS 301
           LAC WAYEGVEAG+TLSE+Y+DSRLPIVMERLA+GGVRLAMLLNRVF+E+   GF  S
Sbjct: 243 LACTWAYEGVEAGMTLSEDYFDSRLPIVMERLAKGGVRLAMLLNRVFSENPKGGFRSS 298

[Close]

BLAST of Chy12G215710 vs. NCBI nr
Match: XP_022927405.1 (endonuclease 1 [Cucurbita moschata])

HSP 1 Score: 479 bits (1233), Expect = 1.65e-168
Identity = 230/298 (77.18%), Postives = 256/298 (85.91%), Query Frame = 0


Query: 5   VFRFLVVLSFISFLLVLPCAQGWSKEGHILTCEIAQELLIPEAAEAVQDLLPESAGGNLS 64
           VFRFLV+L F +FLL LP AQGWSKEGH+LTC+IAQELL PEA EAVQ LLPESAGGNLS
Sbjct: 3   VFRFLVLLCF-TFLL-LPSAQGWSKEGHVLTCQIAQELLNPEATEAVQALLPESAGGNLS 62

Query: 65  AMCIWPDQIRLQSKYRWASPLHYANTPDS-CSFVYKRDCHNDAGQPDMCVAGAIRNFTTQ 124
           AMC+W DQIR  SKYRW SPLHY NTPD+ CSF+YKRDCHN A Q +MCVAGAIRNFTTQ
Sbjct: 63  AMCVWADQIRRWSKYRWTSPLHYINTPDNACSFLYKRDCHNTAAQVNMCVAGAIRNFTTQ 122

Query: 125 LTTYRTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHV 184
           LT +  QG D+ +NLTEALLFLSHFVGDIHQPLHVGF SD GGNTIE+RW+R KSNLHHV
Sbjct: 123 LTAFPKQGPDAKNNLTEALLFLSHFVGDIHQPLHVGFTSDEGGNTIELRWYRHKSNLHHV 182

Query: 185 WDRDIILEALGDYYDKDGGLLLDELDRNLTAGIWSNDVPVWERCPTINSCVNKWADESTA 244
           WDR+IIL AL DYYDKD  LLL++L RNLT GIWS+DVP WERC  +NSC+N WA+ES  
Sbjct: 183 WDREIILTALADYYDKDTDLLLEDLQRNLTHGIWSDDVPTWERCVNVNSCINNWAEESIK 242

Query: 245 LACKWAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFAEDATRGFAYS 301
           LAC WAYEGVEAG+TLSE+Y+DSRLPIVMERLA+GGVRLAMLLNRVF+E+   GF  S
Sbjct: 243 LACTWAYEGVEAGMTLSEDYFDSRLPIVMERLAKGGVRLAMLLNRVFSENPKGGFRSS 298

[Close]

BLAST of Chy12G215710 vs. TAIR 10
Match: AT1G11190.1 (bifunctional nuclease i )

HSP 1 Score: 362.8 bits (930), Expect = 2.6e-100
Identity = 166/286 (58.04%), Postives = 217/286 (75.87%), Query Frame = 0


Query: 9   LVVLSFISFLLVLPCAQGWSKEGHILTCEIAQELLIPEAAEAVQDLLPESAGGNLSAMCI 68
           L+++  I  L  +   + WSKEGHILTC IAQ LL    A  V++LLP+   G+LSA+C+
Sbjct: 11  LILVLGILILCSVSSVRSWSKEGHILTCRIAQNLLEAGPAHVVENLLPDYVKGDLSALCV 70

Query: 69  WPDQIRLQSKYRWASPLHYANTPD-SCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLTTY 128
           WPDQIR   KYRW S LHY +TPD +CS+ Y RDCH+  G  DMCV GAI+NFT+QL  Y
Sbjct: 71  WPDQIRHWYKYRWTSHLHYIDTPDQACSYEYSRDCHDQHGLKDMCVDGAIQNFTSQLQHY 130

Query: 129 RTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHVWDRD 188
                D  +N+TEALLFLSHF+GDIHQP+HVGF SD GGNTI++RW++ KSNLHHVWDR+
Sbjct: 131 GEGTSDRRYNMTEALLFLSHFMGDIHQPMHVGFTSDEGGNTIDLRWYKHKSNLHHVWDRE 190

Query: 189 IILEALGDYYDKDGGLLLDELDRNLTAGIWSNDVPVWERCPTINSCVNKWADESTALACK 248
           IIL AL + YDK+  LL ++L++N+T G+W +D+  W  C  + +C +K+A ES  LACK
Sbjct: 191 IILTALKENYDKNLDLLQEDLEKNITNGLWHDDLSSWTECNDLIACPHKYASESIKLACK 250

Query: 249 WAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFAED 294
           W Y+GV++G TLSEEY+++RLPIVM+R+ QGGVRLAM+LNRVF++D
Sbjct: 251 WGYKGVKSGETLSEEYFNTRLPIVMKRIVQGGVRLAMILNRVFSDD 296

[Close]

BLAST of Chy12G215710 vs. TAIR 10
Match: AT1G68290.1 (endonuclease 2 )

HSP 1 Score: 315.8 bits (808), Expect = 3.6e-86
Identity = 148/283 (52.30%), Postives = 199/283 (70.32%), Query Frame = 0


Query: 9   LVVLSFISFLLVLPCAQGWSKEGHILTCEIAQELLIPEAAEAVQDLLPESAGGNLSAMCI 68
           ++++  +  L   P   GW KEGH + C+IAQ  L   AA+AV++LLPESA G+LS++C+
Sbjct: 10  VMMIITVWLLYAAPNIHGWGKEGHEIICKIAQTRLDETAAKAVKELLPESAEGDLSSLCL 69

Query: 69  WPDQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLTTYR 128
           W D+++   +Y W+SPLHY NTPD+CS+ Y RDC +++G+   CVAGAI N+TTQL +Y+
Sbjct: 70  WADRVKF--RYHWSSPLHYINTPDACSYQYNRDCKDESGEKGRCVAGAIYNYTTQLLSYK 129

Query: 129 T-QGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHVWDRD 188
           T     S +NLTEALLF+SHF+GDIHQPLHV + SD GGNTIEV W+ RK+NLHH+WD +
Sbjct: 130 TAASSQSQYNLTEALLFVSHFMGDIHQPLHVSYASDKGGNTIEVHWYTRKANLHHIWDSN 189

Query: 189 IILEALGDYYDKDGGLLLDELDRNLTAGIWSNDVPVWERCPTINSCVNKWADESTALACK 248
           II  A  D Y+     ++D L +N+T   W++ V  WE C    +C + +A E    AC 
Sbjct: 190 IIETAEADLYNSALEGMVDALKKNITTE-WADQVKRWETCTKKTACPDIYASEGIQAACD 249

Query: 249 WAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVF 291
           WAY+GV  G TL +EY+ SRLPIV +RLAQGGVRLA  LNR+F
Sbjct: 250 WAYKGVTEGDTLEDEYFYSRLPIVYQRLAQGGVRLAATLNRIF 289

[Close]

BLAST of Chy12G215710 vs. TAIR 10
Match: AT4G21585.1 (endonuclease 4 )

HSP 1 Score: 303.5 bits (776), Expect = 1.9e-82
Identity = 146/268 (54.48%), Postives = 183/268 (68.28%), Query Frame = 0


Query: 27  WSKEGHILTCEIAQELLIPEAAEAVQDLLPESAGGNLSAMCIWPDQIRLQSKYRWASPLH 86
           W KEGH   C+IA+     E   AV+ LLP+SA G+L+++C WPD+I+   ++RW SPLH
Sbjct: 25  WGKEGHYTVCKIAESYFEEETVAAVKKLLPKSADGDLASVCSWPDEIKHHWQWRWTSPLH 84

Query: 87  YANTPD-SCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLTTYRTQGFDSPH-NLTEALLF 146
           Y +TPD  C++ Y RDCH+     D CV GAI N+T QL +         H NLTEAL+F
Sbjct: 85  YVDTPDYRCNYEYCRDCHDTHKNQDRCVTGAIFNYTMQLMSASENSDTIVHYNLTEALMF 144

Query: 147 LSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHVWDRDIILEALGDYYDKDGGLL 206
           LSHF+GDIHQPLHVGF  D GGNTI VRW+RRK+NLHHVWD  II  AL  YY+K   L+
Sbjct: 145 LSHFIGDIHQPLHVGFLGDEGGNTITVRWYRRKTNLHHVWDNMIIESALKTYYNKSLPLM 204

Query: 207 LDELDRNLTAGIWSNDVPVWERCP-TINSCVNKWADESTALACKWAYEGVEAGITLSEEY 266
           ++ L  NLT   WSNDVP+WE C     +C N +A ES  LACK+AY     G TL ++Y
Sbjct: 205 IEALQANLT-NDWSNDVPLWESCQLNQTACPNPYASESINLACKYAYRNATPGTTLGDDY 264

Query: 267 YDSRLPIVMERLAQGGVRLAMLLNRVFA 292
           + SRLPIV +RLAQGG+RLA  LNR+F+
Sbjct: 265 FLSRLPIVEKRLAQGGIRLAATLNRIFS 291

[Close]

BLAST of Chy12G215710 vs. TAIR 10
Match: AT4G21600.1 (endonuclease 5 )

HSP 1 Score: 275.0 bits (702), Expect = 7.1e-74
Identity = 139/287 (48.43%), Postives = 179/287 (62.37%), Query Frame = 0


Query: 12  LSFISFLLVLPCAQG---WSKEGHILTCEIAQELLIPEAAEAVQDLLPESA-GGNLSAMC 71
           L  +S L++     G   W K+GH   C++A+     +   AV+ LLPES  GG L+  C
Sbjct: 3   LWIVSVLVLTHLVHGALCWGKDGHYTVCKLAEGFFEDDTIAAVKKLLPESVDGGGLADFC 62

Query: 72  IWPDQIRLQSKYRWASPLHYANTPD-SCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLTT 131
            WPD+I+  S+++W S LHY NTP+  C++ Y RDCH+     D CV GAI N+T QL +
Sbjct: 63  SWPDEIKKLSQWQWTSTLHYVNTPEYRCNYEYCRDCHDTHKHKDWCVTGAIFNYTNQLMS 122

Query: 132 YRTQGFDSPH-NLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHVWD 191
                 +  H NLTEALLFLSH++GD+HQPLH GF  D GGNTI V W+  KSNLHHVWD
Sbjct: 123 ASENSQNIVHYNLTEALLFLSHYMGDVHQPLHTGFLGDLGGNTIIVNWYHNKSNLHHVWD 182

Query: 192 RDIILEALGDYYDKDGGLLLDELDRNLTAGIWSNDVPVWERCP-TINSCVNKWADESTAL 251
             II  AL  YY+     ++  L   L  G WSNDVP W+ C     +C N +A ES  L
Sbjct: 183 NMIIDSALETYYNSSLPHMIQALQAKLKNG-WSNDVPSWKSCHFHQKACPNLYASESIDL 242

Query: 252 ACKWAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFA 292
           ACK+AY     G TL +EY+ SRLP+V +RLAQGG+RLA  LNR+F+
Sbjct: 243 ACKYAYRNATPGTTLGDEYFLSRLPVVEKRLAQGGIRLAATLNRIFS 288

[Close]

BLAST of Chy12G215710 vs. TAIR 10
Match: AT4G21590.1 (endonuclease 3 )

HSP 1 Score: 270.0 bits (689), Expect = 2.3e-72
Identity = 137/283 (48.41%), Postives = 176/283 (62.19%), Query Frame = 0


Query: 15  ISFLLVLPCAQG---WSKEGHILTCEIAQELLIPEAAEAVQDLLPESAGGNLSAMCIWPD 74
           +S L++     G   W   GH   C+IAQ     +   AV+ LLPESA G L+A+C WPD
Sbjct: 10  VSILVLTQLVNGALCWGDAGHYAVCKIAQSYFEEDTVVAVKKLLPESANGELAAVCSWPD 69

Query: 75  QIRLQSKYRWASPLHYANTPD-SCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLTTYRTQ 134
           +I+   ++RW S LH+A+TPD  C++ Y RDC       D CV GAI N+T QL +    
Sbjct: 70  EIKKLPQWRWTSALHFADTPDYKCNYEYSRDC-----PKDWCVTGAIFNYTNQLMSTSEN 129

Query: 135 GFDSPH-NLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHVWDRDII 194
                H NLTEAL+FLSH++GDIHQPLH GF  D GGN I+V W+ +++NLH VWD  II
Sbjct: 130 SQSIVHYNLTEALMFLSHYMGDIHQPLHEGFIGDLGGNKIKVHWYNQETNLHRVWDDMII 189

Query: 195 LEALGDYYDKDGGLLLDELDRNLTAGIWSNDVPVWERCP-TINSCVNKWADESTALACKW 254
             AL  YY+     ++ EL   L  G WSNDVP WE C     +C N +A ES  LACK+
Sbjct: 190 ESALETYYNSSLPRMIHELQAKLKNG-WSNDVPSWESCQLNQTACPNPYASESIDLACKY 249

Query: 255 AYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFA 292
           AY    AG TL + Y+ SRLP+V +RLAQGG+RLA  LNR+F+
Sbjct: 250 AYRNATAGTTLGDYYFVSRLPVVEKRLAQGGIRLAGTLNRIFS 286

The following BLAST results are available for this feature:

BLAST of Chy12G215710 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SXA6	3.7e-99	58.04	Endonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=ENDO1 PE=1 SV=1	[more]
Q9C9G4	5.1e-85	52.30	Endonuclease 2 OS=Arabidopsis thaliana OX=3702 GN=ENDO2 PE=1 SV=1	[more]
F4JJL0	2.6e-81	54.48	Endonuclease 4 OS=Arabidopsis thaliana OX=3702 GN=ENDO4 PE=1 SV=1	[more]
F4JJL3	1.0e-72	48.43	Endonuclease 5 OS=Arabidopsis thaliana OX=3702 GN=ENDO5 PE=1 SV=1	[more]
Q8LDW6	3.2e-71	48.41	Endonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=ENDO3 PE=1 SV=1	[more]

BLAST of Chy12G215710 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
C3VEY2	1.1e-170	96.03	Aspergillus nuclease S(1) OS=Cucumis sativus OX=3659 PE=2 SV=2	[more]
A0A5D3DSP7	8.0e-166	92.38	Aspergillus nuclease S(1) OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]
A0A1S3B4B0	8.0e-166	92.38	Aspergillus nuclease S(1) OS=Cucumis melo OX=3656 GN=LOC103486022 PE=3 SV=1	[more]
A0A0A0LUG7	7.8e-137	96.20	Aspergillus nuclease S(1) OS=Cucumis sativus OX=3659 GN=Csa_1G072450 PE=3 SV=1	[more]
A0A6J1ENU3	3.4e-132	76.51	Aspergillus nuclease S(1) OS=Cucurbita moschata OX=3662 GN=LOC111434241 PE=3 SV=...	[more]

BLAST of Chy12G215710 vs. NCBI nr


Match Name	E-value	Identity	Description
NP_001292654.1	8.89e-219	96.03	endonuclease 1 precursor [Cucumis sativus] >ACO72982.2 bifunctional nuclease pre...	[more]
XP_008442043.1	2.42e-212	92.38	PREDICTED: endonuclease 1 [Cucumis melo] >KAA0056948.1 endonuclease 1 [Cucumis m...	[more]
XP_038895329.1	6.39e-182	81.79	LOW QUALITY PROTEIN: endonuclease 1-like [Benincasa hispida]	[more]
KAG6584021.1	1.17e-168	77.18	Endonuclease 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019639.1 En...	[more]
XP_022927405.1	1.65e-168	77.18	endonuclease 1 [Cucurbita moschata]	[more]

BLAST of Chy12G215710 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G11190.1	2.6e-100	58.04	bifunctional nuclease i	[more]
AT1G68290.1	3.6e-86	52.30	endonuclease 2	[more]
AT4G21585.1	1.9e-82	54.48	endonuclease 4	[more]
AT4G21600.1	7.1e-74	48.43	endonuclease 5	[more]
AT4G21590.1	2.3e-72	48.41	endonuclease 3	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003154	S1/P1 nuclease	PFAM	PF02265	S1-P1_nuclease	coord: 27..290 e-value: 1.5E-80 score: 270.7
IPR003154	S1/P1 nuclease	PANTHER	PTHR33146	ENDONUCLEASE 4	coord: 6..296
IPR003154	S1/P1 nuclease	CDD	cd11010	S1-P1_nuclease	coord: 27..290 e-value: 3.04682E-89 score: 264.632
IPR008947	Phospholipase C/P1 nuclease domain superfamily	GENE3D	1.10.575.10	P1 Nuclease	coord: 27..295 e-value: 1.0E-95 score: 322.6
IPR008947	Phospholipase C/P1 nuclease domain superfamily	SUPERFAMILY	48537	Phospholipase C/P1 nuclease	coord: 27..288
None	No IPR available	PANTHER	PTHR33146:SF14	ENDONUCLEASE 1	coord: 6..296

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy12G215710.1	Chy12G215710.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006308	DNA catabolic process
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004521	endoribonuclease activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0000014	single-stranded DNA endodeoxyribonuclease activity
molecular_function	GO:0004519	endonuclease activity
molecular_function	GO:0016788	hydrolase activity, acting on ester bonds