Chy12G215130 (gene) Cucumber (hystrix) v1

BLAST of Chy12G215130 vs. ExPASy Swiss-Prot
Match: Q9SGN6 (MACPF domain-containing protein NSL1 OS=Arabidopsis thaliana OX=3702 GN=NSL1 PE=2 SV=1)

HSP 1 Score: 789.6 bits (2038), Expect = 2.4e-227
Identity = 391/612 (63.89%), Postives = 474/612 (77.45%), Query Frame = 0

Query: 4   NINRFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPAGVV 63
           N  R +  SAAEKAVSVIG GYDLC+D+R S+CK  P GS+L++ID TR+RDL+ P G+V
Sbjct: 5   NFTRLDAHSAAEKAVSVIGLGYDLCSDVRFSACKTTPDGSRLVEIDPTRNRDLIFPGGIV 64

Query: 64  VPNVPTSINCDKGERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLKGCWQND 123
           V NV +SI CDKGERTR RSD++SFNQMSE  NQ + LSGKIPSG FN MF    CW  D
Sbjct: 65  VNNVSSSIKCDKGERTRLRSDILSFNQMSEKFNQDMCLSGKIPSGMFNNMFAFSKCWPKD 124

Query: 124 AGSTRSLAFDGCFITLYNIELEKSHLTLSEQVKREVPSSWDPAALAEFIEKYGTHVVVGV 183
           A S ++LA+DG FI+LY++E+ +  LTL ++VKREVPSSWD AALA FIEKYGTHVVVGV
Sbjct: 125 ASSVKTLAYDGWFISLYSVEIVRKQLTLRDEVKREVPSSWDSAALAGFIEKYGTHVVVGV 184

Query: 184 KMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSED-ANGGLTSNPVAAKIKDEYSLP 243
            MGGKDVI ++Q+R+SN E  ++QK LK   D++F  D       ++  + K K+E  L 
Sbjct: 185 TMGGKDVIHVKQMRKSNHEPEEIQKMLKHWGDERFCVDPVESKSPASVYSGKPKEENLLQ 244

Query: 244 WELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSFVP 303
           W L+  F  S+   V+ H+K +E+  + IRRGGVD+GQSH +WLST+SQ+PNVISM FVP
Sbjct: 245 WGLQ-PFGTSVSSAVVMHTKNEEIMRVCIRRGGVDLGQSHERWLSTVSQAPNVISMCFVP 304

Query: 304 ITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRKQA 363
           ITSL++G+ G GFLSHAVNLYLRYKPPIEEL QFLEFQLPRQWAPVY DLPL  R  KQ+
Sbjct: 305 ITSLLSGLPGTGFLSHAVNLYLRYKPPIEELHQFLEFQLPRQWAPVYGDLPLGLRRSKQS 364

Query: 364 SPSLKFTLMGPKLYVNTTKVDSNNHPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQLSD 423
           SPSL+F+LMGPKLYVNT+KVDS   PVTG+R FLEGKK +HLAIHLQHLS  P +L LS 
Sbjct: 365 SPSLQFSLMGPKLYVNTSKVDSGERPVTGLRFFLEGKKGNHLAIHLQHLSACPPSLHLSH 424

Query: 424 DLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKGIGMR 483
           D +Y PI+EP EKGY+ PVKW IFSHVCT PV+YNG+   DD+ASIVTKAW EVKG+GMR
Sbjct: 425 DDTYEPIEEPVEKGYYVPVKWGIFSHVCTYPVQYNGAR-SDDTASIVTKAWLEVKGMGMR 484

Query: 484 KVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLH----QPEEQPKI 543
           KVLFLRLGFS+ ASA  R+S W+  ST SRKSG+ S  ISTR S GL       + Q KI
Sbjct: 485 KVLFLRLGFSLDASAVTRKSCWDNLSTNSRKSGVFS-MISTRLSTGLSPNPATTKPQSKI 544

Query: 544 ELNSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKY 603
           ++NSAVYP GP    +  K+L+++DTKE++RGPE  PGYWVVTGAKLCVE G+ISIK KY
Sbjct: 545 DINSAVYPRGPSPPVKP-KLLSLVDTKEVMRGPEEQPGYWVVTGAKLCVEAGKISIKAKY 604

Query: 604 SLLTILSEDSMI 611
           SLLT++SEDS++
Sbjct: 605 SLLTVISEDSLV 612

BLAST of Chy12G215130 vs. ExPASy Swiss-Prot
Match: Q9STW5 (MACPF domain-containing protein At4g24290 OS=Arabidopsis thaliana OX=3702 GN=At4g24290 PE=2 SV=1)

HSP 1 Score: 626.3 bits (1614), Expect = 3.6e-178
Identity = 318/610 (52.13%), Postives = 419/610 (68.69%), Query Frame = 0

Query: 7   RFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDI-DFTRSRDLVLPAGVVVP 66
           R     AAE A+  IG GYDL  DLRL  CK G   S+L+DI +   + ++VLP G+ +P
Sbjct: 4   RLPASKAAEVAIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGISIP 63

Query: 67  NVPTSINCDKGERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLKGCWQNDAG 126
           NV  SI CDKGER RFRSD++ F QM+E  NQ+LSL+GKIPSG FN MF    CWQ DA 
Sbjct: 64  NVSKSIKCDKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQKDAA 123

Query: 127 STRSLAFDGCFITLYNIELEKSHLTLSEQVKREVPSSWDPAALAEFIEKYGTHVVVGVKM 186
            T++LAFDG FI+LY++ L+KS + L E VK+ VPS+WDPAALA FI+ YGTH++V VKM
Sbjct: 124 YTKNLAFDGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKM 183

Query: 187 GGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDA---NGGLTSNPVAAKIKDEYSLP 246
           GGKDVI+ +Q   S ++  D+QK+LK++AD +F E +   N G      ++K++ +    
Sbjct: 184 GGKDVIYAKQQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKEQ-- 243

Query: 247 WELRNAFAASIKPPVINHSKGDELTAIYIRRGGVD-VGQSHNQWLSTISQSPNVISMSFV 306
             LR A  +S+     +++  ++   +  RRGG D     HN+WL T+   P+VISMSF+
Sbjct: 244 -RLRFADTSSLG----SYANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFI 303

Query: 307 PITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRKQ 366
           PITSL+NGV G+GFLSHA+NLYLRYKPPIEEL QFLEFQLPRQWAPV+++LPL P+ ++Q
Sbjct: 304 PITSLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQRKQQ 363

Query: 367 ASPSLKFTLMGPKLYVNTTKVDSNNHPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQLS 426
           +  SL+F+  GPKLYVNTT VD    P+TG+RL+LEG++S+ LAIHLQHLS+LPK  QL 
Sbjct: 364 SCASLQFSFFGPKLYVNTTPVDVGKRPITGMRLYLEGRRSNRLAIHLQHLSSLPKIYQLE 423

Query: 427 DDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKGIGM 486
           DDL+     E  ++ Y+E V W  +SHVCT PVE       DD  S+VT A   V+  G 
Sbjct: 424 DDLNRSIRQESHDRRYYEKVNWKNYSHVCTEPVE------SDDDLSVVTGAQLHVESHGF 483

Query: 487 RKVLFLRLGFSMVASAG-IRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL 546
           + VLFLRL FS V  A  ++ S+W+     + KSGLIST IS  F+A    P     + +
Sbjct: 484 KNVLFLRLCFSRVVGATLVKNSEWDEAVGFAPKSGLISTLISHHFTAAQKPPPRPADVNI 543

Query: 547 NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL 606
           NSA+YP GPP+ ++A K+L  +DT EM RGP+ SPGYWVV+GA+L VE G+IS+KVKYSL
Sbjct: 544 NSAIYPGGPPVPTQAPKLLKFVDTSEMTRGPQESPGYWVVSGARLLVEKGKISLKVKYSL 600

Query: 607 LTILSEDSMI 611
            T +  D +I
Sbjct: 604 FTPILGDEVI 600

BLAST of Chy12G215130 vs. ExPASy Swiss-Prot
Match: Q8L612 (MACPF domain-containing protein At1g14780 OS=Arabidopsis thaliana OX=3702 GN=At1g14780 PE=2 SV=1)

HSP 1 Score: 466.1 bits (1198), Expect = 6.2e-130
Identity = 258/614 (42.02%), Postives = 377/614 (61.40%), Query Frame = 0

Query: 15  EKAVSVIGFGYDLCNDLRLSSCKPGPSGS---KLIDIDFTRSRDLVLPAGVVVPNVPTSI 74
           E AV  +G G+DL  D RL  CK G   +   +L+ +D T++R+L +P   V  NV   I
Sbjct: 10  ETAVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFGVFQNVSADI 69

Query: 75  NCDKGERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLK-GCWQNDAGSTRSL 134
           NCDKGERTRFRSD++ FN+MSE  NQ+ S++GKIPSG FN  FG + G W  DA + +SL
Sbjct: 70  NCDKGERTRFRSDILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWATDAANVKSL 129

Query: 135 AFDGCFITLYNIELEK-SHLTLSEQVKREVPSSWDPAALAEFIEKYGTHVVVGVKMGGKD 194
             D   +TL+N+ +   + L L+++V+  VPSSWDP  LA FIE+YGTHV+ GV +GG+D
Sbjct: 130 GLDASVVTLFNLHIHNPNRLRLTDRVRNAVPSSWDPQLLARFIERYGTHVITGVSVGGQD 189

Query: 195 VIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGLTSNPVAAKIKDEYSLPW--ELRN 254
           V+ +RQ + S+++   ++  L  L D  F+      L++  +       +S P   E  N
Sbjct: 190 VVVVRQDKSSDLDNDLLRHHLYDLGDQLFTGSCL--LSTRRLNKAYHHSHSQPKFPEAFN 249

Query: 255 AF----AASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSFVPI 314
            F      +     IN   G  +T I  +RGG    +SH++WL T+   P+ I+ +F+PI
Sbjct: 250 VFDDKQTVAFNNFSINSQNG--ITVICAKRGGDGRAKSHSEWLITVPDKPDAINFNFIPI 309

Query: 315 TSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPL-APRHRKQA 374
           TSL+  V G+G LSHA++LYLRYKPP+ +LQ FL+F  PR WAPV+ DLP  A  +   A
Sbjct: 310 TSLLKDVPGSGLLSHAMSLYLRYKPPLMDLQYFLDFSGPRAWAPVHNDLPFGAAPNMASA 369

Query: 375 SPSLKFTLMGPKLYVNTTKVDSNNHPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTL--QL 434
            P+L    MGPKLYVNTT V S  +PVTG+R FLEGKK + LAIHLQHL     T+  ++
Sbjct: 370 YPALHINFMGPKLYVNTTPVTSEKNPVTGMRFFLEGKKCNRLAIHLQHLDNTRTTVGEKI 429

Query: 435 SDDLSYRPIDE-PEEKGYFEPVKWSIFSHVCTAPVEYNGSLI--------DDDSASIVTK 494
           +D+  +R  D+  +   YFEP+    FSHVCT PV+Y+ + I         +D A IVT 
Sbjct: 430 TDEHIWRGSDQITDNDRYFEPLNGKKFSHVCTVPVKYDPNWIKTTSNHKSQNDVAFIVTG 489

Query: 495 AWFEVKGIGMRKVLFLRLGFSMVASAGIRRSDW-EGPSTTSRKSGLISTFISTRFSAGLH 554
           A  EVK  G + VL LRL ++ V+   + ++ W  GP  TS+KSG+ S+      S  +H
Sbjct: 490 AQLEVKKHGSKSVLHLRLRYTKVSDHYVVQNSWVHGPIGTSQKSGIFSSMSMPLTSGSVH 549

Query: 555 Q---PEEQPKIELNSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCV 602
                +++ ++ L+S V+P GPP+ +   K++  +D  ++ RGP++SPG+W+VTG +L +
Sbjct: 550 HNMIQKDKNEVVLDSGVFPGGPPVPAN-NKIVKFVDLSQLCRGPQHSPGHWLVTGVRLYL 609

BLAST of Chy12G215130 vs. ExPASy Swiss-Prot
Match: Q9C7N2 (MACPF domain-containing protein CAD1 OS=Arabidopsis thaliana OX=3702 GN=CAD1 PE=2 SV=1)

HSP 1 Score: 426.4 bits (1095), Expect = 5.4e-118
Identity = 239/600 (39.83%), Postives = 348/600 (58.00%), Query Frame = 0

Query: 17  AVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPAGVVVPNVPTSINCDKG 76
           A+  +G G+D+ +D+RL  CK  P GS+L+ I+  ++RDL L  G ++PNVP  I+C +G
Sbjct: 24  AIQALGRGFDVTSDVRLLYCKGAP-GSRLVRIEEGQNRDLELSHGFLLPNVPADIDCSRG 83

Query: 77  ERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLKGCWQNDAGSTRSLAFDGCF 136
                R  V SF++M+E  N +  + G IP G FN MF   G WQ DA ST+SLA  G F
Sbjct: 84  NSGTQRISVCSFHEMAEEFNVRSGVKGNIPLGCFNAMFNYTGSWQVDAASTKSLALVGYF 143

Query: 137 ITLYNIELEKSHLTLSEQVKREVPSSWDPAALAEFIEKYGTHVVVGVKMGGKDVIFMRQL 196
           I LY+++L K  L L  +++R VPSSWDPA+LA FIE YGTH+V  V +GG+DV+++RQ 
Sbjct: 144 IPLYDVKLAKLTLVLHNEIRRAVPSSWDPASLASFIENYGTHIVTSVTIGGRDVVYIRQH 203

Query: 197 RESNVEQTDVQKKLKKLADDKFSEDANGGLTSNPVAAKIKDEYSLPWELRNAFAASIKPP 256
           + S +  ++++  +  +   +F E  +  +T  P+  K KD                   
Sbjct: 204 QSSPLPVSEIENYVNDMIKHRFHEAESQSIT-GPLKYKDKD------------------- 263

Query: 257 VINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSFVPITSLMNGVRGNGFL 316
                    +T I+ RRGG D+ QSH +W  T+  +P++I+M+F PI SL+ GV G   L
Sbjct: 264 ---------ITVIFRRRGGDDLEQSHARWAETVPAAPDIINMTFTPIVSLLEGVPGLRHL 323

Query: 317 SHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRKQASPSLKFTLMGPKLY 376
           + A+ LYL YKPPIE+LQ FL++Q+ R WAP  ++L    + ++    SL+F+LMGPKL+
Sbjct: 324 TRAIELYLEYKPPIEDLQYFLDYQIARAWAPEQSNL----QRKEPVCSSLQFSLMGPKLF 383

Query: 377 VNTTKVDSNNHPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQLSDDLSYRPIDEPEEKG 436
           ++  +V     PVTG+RL LEG K + L+IHLQHL +LPK LQ   D S+ PI  P+ +G
Sbjct: 384 ISADQVTVGRKPVTGLRLSLEGSKQNRLSIHLQHLVSLPKILQPHWD-SHVPIGAPKWQG 443

Query: 437 -------YFEPVKWSIFSHVCTAPVEYNGSLIDD-DSASIVTKAWFEVKGIGMRKVLFLR 496
                  +FEP+KW  FSHV T+P+E+  + I D     IVT A   V   G + VL L+
Sbjct: 444 PEEQDSRWFEPIKWKNFSHVSTSPIEHTETHIGDLSGVHIVTGAQLGVWDFGSKNVLHLK 503

Query: 497 LGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIELNSAVYPDG 556
           L FS V    IRRS W+                        H P     +  +  + P G
Sbjct: 504 LLFSKVPGCTIRRSVWD------------------------HTP-----VASSGRLEPGG 559

Query: 557 PPLTS-------RAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSLL 602
           P  +S       ++ K+  ++D+ EM++GP++ PG+W+VTGAKL VE G+I ++VKYSLL
Sbjct: 564 PSTSSSTEEVSGQSGKLAKIVDSSEMLKGPQDLPGHWLVTGAKLGVEKGKIVLRVKYSLL 559

BLAST of Chy12G215130 vs. ExPASy TrEMBL
Match: A0A0A0LV66 (MACPF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G075560 PE=4 SV=1)

HSP 1 Score: 1232.2 bits (3187), Expect = 0.0e+00
Identity = 608/610 (99.67%), Postives = 609/610 (99.84%), Query Frame = 0

Query: 1   MSFNINRFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA 60
           MSFNINRFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA
Sbjct: 1   MSFNINRFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA 60

Query: 61  GVVVPNVPTSINCDKGERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLKGCW 120
           GVVVPNVPTSINCDKGERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLKGCW
Sbjct: 61  GVVVPNVPTSINCDKGERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLKGCW 120

Query: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHLTLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180
           QNDAGSTRSLAFDGCFITLYNIELEKSH+TLSEQVKREVPSSWDPAALAEFIEKYGTHVV
Sbjct: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180

Query: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGLTSNPVAAKIKDEYS 240
           VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGLTSNPVAAKIKDEYS
Sbjct: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGLTSNPVAAKIKDEYS 240

Query: 241 LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF 300
           LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF
Sbjct: 241 LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF 300

Query: 301 VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK 360
           VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK
Sbjct: 301 VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK 360

Query: 361 QASPSLKFTLMGPKLYVNTTKVDSNNHPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL 420
           QASPSLKFTLMGPKLYVNTTKVDSNN PVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL
Sbjct: 361 QASPSLKFTLMGPKLYVNTTKVDSNNLPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL 420

Query: 421 SDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKGIG 480
           SDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKGIG
Sbjct: 421 SDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKGIG 480

Query: 481 MRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL 540
           MRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL
Sbjct: 481 MRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL 540

Query: 541 NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL 600
           NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL
Sbjct: 541 NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL 600

Query: 601 LTILSEDSMI 611
           LTILSEDSMI
Sbjct: 601 LTILSEDSMI 610

BLAST of Chy12G215130 vs. ExPASy TrEMBL
Match: A0A5D3DSF4 (MACPF domain-containing protein NSL1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861G001020 PE=4 SV=1)

HSP 1 Score: 1216.1 bits (3145), Expect = 0.0e+00
Identity = 601/610 (98.52%), Postives = 604/610 (99.02%), Query Frame = 0

Query: 1   MSFNINRFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA 60
           MSFNINRFNPQSAAEKAVSV+GFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA
Sbjct: 1   MSFNINRFNPQSAAEKAVSVVGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA 60

Query: 61  GVVVPNVPTSINCDKGERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLKGCW 120
           GVVVPNVPTSINCDKGERTRFRSDVISFNQMSEL NQQLSLSGKIPSGFFNTMFGLKGCW
Sbjct: 61  GVVVPNVPTSINCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSGFFNTMFGLKGCW 120

Query: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHLTLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180
           QNDAGSTRSLAFDGCFITLYNIELEKSH+TLSEQVKREVPSSWDPAALAEFIEKYGTHVV
Sbjct: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180

Query: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGLTSNPVAAKIKDEYS 240
           VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGG TSN VAAKIKDEYS
Sbjct: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGFTSNSVAAKIKDEYS 240

Query: 241 LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF 300
           LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF
Sbjct: 241 LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF 300

Query: 301 VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK 360
           VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK
Sbjct: 301 VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK 360

Query: 361 QASPSLKFTLMGPKLYVNTTKVDSNNHPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL 420
           QASPSLKFTLMGPKLYVNTTKVDSNN PVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL
Sbjct: 361 QASPSLKFTLMGPKLYVNTTKVDSNNRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL 420

Query: 421 SDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKGIG 480
           SDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEY+ SLIDDDSASIVTKAWFEVK IG
Sbjct: 421 SDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYHDSLIDDDSASIVTKAWFEVKVIG 480

Query: 481 MRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL 540
           MRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL
Sbjct: 481 MRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL 540

Query: 541 NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL 600
           NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL
Sbjct: 541 NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL 600

Query: 601 LTILSEDSMI 611
           LTILSEDSMI
Sbjct: 601 LTILSEDSMI 610

BLAST of Chy12G215130 vs. ExPASy TrEMBL
Match: A0A5A7UMD4 (MACPF domain-containing protein NSL1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold96G001940 PE=4 SV=1)

HSP 1 Score: 1145.6 bits (2962), Expect = 0.0e+00
Identity = 576/610 (94.43%), Postives = 585/610 (95.90%), Query Frame = 0

Query: 1   MSFNINRFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA 60
           MSFNINRFNPQSAAEKAVSV+GFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA
Sbjct: 1   MSFNINRFNPQSAAEKAVSVVGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA 60

Query: 61  GVVVPNVPTSINCDKGERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLKGCW 120
           GVVVPNVPTSINCDKGERTRFRSDVISFNQMSEL NQQLSLSGKIPSGFFNTMFGLKGCW
Sbjct: 61  GVVVPNVPTSINCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSGFFNTMFGLKGCW 120

Query: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHLTLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180
           QNDAGSTRSLAFDGCFITLYNIELEKSH+TLSEQVKREVPSSWDPAALAEFIEKYGTHVV
Sbjct: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180

Query: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGLTSNPVAAKIKDEYS 240
           VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGG TSN VAAKIK    
Sbjct: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGFTSNSVAAKIK---- 240

Query: 241 LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF 300
           +P +  + + +   P   +H    ELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF
Sbjct: 241 VPTQ-EHKWLSPFGPFHWSH----ELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF 300

Query: 301 VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK 360
           VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK
Sbjct: 301 VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK 360

Query: 361 QASPSLKFTLMGPKLYVNTTKVDSNNHPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL 420
           QASPSLKFTLMGPKLYVNTTKVDSNN PVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL
Sbjct: 361 QASPSLKFTLMGPKLYVNTTKVDSNNRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL 420

Query: 421 SDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKGIG 480
           SDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEY+ SLIDDDSASIVTKAWFEVK IG
Sbjct: 421 SDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYHDSLIDDDSASIVTKAWFEVKVIG 480

Query: 481 MRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL 540
           MRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL
Sbjct: 481 MRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL 540

Query: 541 NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL 600
           NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL
Sbjct: 541 NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL 600

Query: 601 LTILSEDSMI 611
           LTILSEDSMI
Sbjct: 601 LTILSEDSMI 601

BLAST of Chy12G215130 vs. ExPASy TrEMBL
Match: A0A6J1JYX1 (MACPF domain-containing protein NSL1 OS=Cucurbita maxima OX=3661 GN=LOC111490102 PE=4 SV=1)

HSP 1 Score: 1137.1 bits (2940), Expect = 0.0e+00
Identity = 554/610 (90.82%), Postives = 583/610 (95.57%), Query Frame = 0

Query: 1   MSFNINRFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA 60
           MSFN+NRF+PQSAAEKAVSVIGFG+DLCNDLRLSSCKPGPSGSKLIDID TR++DLVLPA
Sbjct: 1   MSFNVNRFDPQSAAEKAVSVIGFGFDLCNDLRLSSCKPGPSGSKLIDIDLTRTKDLVLPA 60

Query: 61  GVVVPNVPTSINCDKGERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLKGCW 120
           GVVVPNV  SI CDKGERTRFRSDVISFNQMSEL NQQLSLSGKIPS FFN+MFGLKGCW
Sbjct: 61  GVVVPNVSNSIKCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSAFFNSMFGLKGCW 120

Query: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHLTLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180
           QNDAGS++SL FDGCFITLYNIELE+SH+TLSEQVKREVPSSWDPAALA+FIE YGTHVV
Sbjct: 121 QNDAGSSKSLGFDGCFITLYNIELERSHITLSEQVKREVPSSWDPAALAKFIENYGTHVV 180

Query: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGLTSNPVAAKIKDEYS 240
           VGVKMGGKDVIFMRQLRESN+EQT+VQKKLK+LAD++FSEDANGG  SN  AAKIKDEYS
Sbjct: 181 VGVKMGGKDVIFMRQLRESNIEQTEVQKKLKQLADERFSEDANGGFMSNSAAAKIKDEYS 240

Query: 241 LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF 300
           +PW+LR+AFAASIKPP+I HSKGDEL  IY+RRGGVDVGQSHNQWLST+SQSPNVI MSF
Sbjct: 241 MPWQLRHAFAASIKPPIITHSKGDELMTIYVRRGGVDVGQSHNQWLSTVSQSPNVILMSF 300

Query: 301 VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK 360
           VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLA RHRK
Sbjct: 301 VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLATRHRK 360

Query: 361 QASPSLKFTLMGPKLYVNTTKVDSNNHPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL 420
           QASPSLKFTLMGPKLYVNTTKVDSN+ PVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL
Sbjct: 361 QASPSLKFTLMGPKLYVNTTKVDSNSRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL 420

Query: 421 SDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKGIG 480
           SDDLSY PIDEPEEKGYF+PVKWSIFSHVCTAPV+YNGSLIDDD+ASIVTKAWFEVK IG
Sbjct: 421 SDDLSYGPIDEPEEKGYFQPVKWSIFSHVCTAPVQYNGSLIDDDTASIVTKAWFEVKVIG 480

Query: 481 MRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL 540
           MRKVLFLRLGFS VASA IRRS+WEGPST+SRKSGLISTFISTRFS GL QPE QPKIEL
Sbjct: 481 MRKVLFLRLGFSTVASAMIRRSEWEGPSTSSRKSGLISTFISTRFSTGLQQPEAQPKIEL 540

Query: 541 NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL 600
           NSAVYPDGPPLT RAMKMLN+IDTKEMVRGPENSPGYWVVTGAKLCVEG RISIKVKYSL
Sbjct: 541 NSAVYPDGPPLTVRAMKMLNIIDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKYSL 600

Query: 601 LTILSEDSMI 611
           LTILSEDSMI
Sbjct: 601 LTILSEDSMI 610

BLAST of Chy12G215130 vs. ExPASy TrEMBL
Match: A0A6J1GVB1 (MACPF domain-containing protein NSL1 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111457211 PE=4 SV=1)

HSP 1 Score: 1132.5 bits (2928), Expect = 0.0e+00
Identity = 551/610 (90.33%), Postives = 582/610 (95.41%), Query Frame = 0

Query: 1   MSFNINRFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA 60
           MSFN+NRF+PQSAAEKAVSVIGFG+DLCNDLRLSSCKPGPSGSKLIDID TR++DLVLPA
Sbjct: 1   MSFNVNRFDPQSAAEKAVSVIGFGFDLCNDLRLSSCKPGPSGSKLIDIDLTRTKDLVLPA 60

Query: 61  GVVVPNVPTSINCDKGERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLKGCW 120
           GVVVPNV  SI CDKGERTRFRSDV+SFNQMSEL NQQLSLSGKIPS FFN+MFGLKGCW
Sbjct: 61  GVVVPNVSNSIKCDKGERTRFRSDVVSFNQMSELFNQQLSLSGKIPSAFFNSMFGLKGCW 120

Query: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHLTLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180
           QNDAGS++SL FDGCFITLYNIELE+SH+TLSEQVKREVPSSWDPAALA+FIE YGTHVV
Sbjct: 121 QNDAGSSKSLGFDGCFITLYNIELERSHITLSEQVKREVPSSWDPAALAKFIENYGTHVV 180

Query: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGLTSNPVAAKIKDEYS 240
           VGVKMGGKDVIFMRQLRESN+EQ +VQKKLK+LAD++FSED NGG  SN  AAKIKDEYS
Sbjct: 181 VGVKMGGKDVIFMRQLRESNIEQAEVQKKLKQLADERFSEDVNGGFMSNSTAAKIKDEYS 240

Query: 241 LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF 300
           +PW+LR+AFAASIKPP+I HSKGDELT IY+RRGGVDVGQSHNQWLST+SQSPNVISMSF
Sbjct: 241 MPWQLRHAFAASIKPPIITHSKGDELTTIYVRRGGVDVGQSHNQWLSTVSQSPNVISMSF 300

Query: 301 VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK 360
           VPITSL+NGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLA R RK
Sbjct: 301 VPITSLLNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLATRLRK 360

Query: 361 QASPSLKFTLMGPKLYVNTTKVDSNNHPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL 420
           QASPSLKFTLMGPKLYVNTTKVDSN+ PVTGVRLFLEGKKSDHLAIHLQHLSTLPKT+QL
Sbjct: 361 QASPSLKFTLMGPKLYVNTTKVDSNSRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTIQL 420

Query: 421 SDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKGIG 480
           SDDLSY PIDEPEEKGYF+PVKWSIFSHVCTAPV+YNGSLIDDD+ASIVTKAWFEVK IG
Sbjct: 421 SDDLSYGPIDEPEEKGYFQPVKWSIFSHVCTAPVQYNGSLIDDDTASIVTKAWFEVKVIG 480

Query: 481 MRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL 540
           MRKVLFLRLGFS VASA IRRS+WEGPST+SRKSGLISTFISTRFS GL QPE QPKIEL
Sbjct: 481 MRKVLFLRLGFSTVASAKIRRSEWEGPSTSSRKSGLISTFISTRFSTGLQQPEAQPKIEL 540

Query: 541 NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL 600
           NSAVYPDGPPLT RAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEG RISIKVKYSL
Sbjct: 541 NSAVYPDGPPLTVRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKYSL 600

Query: 601 LTILSEDSMI 611
           LTILSEDSMI
Sbjct: 601 LTILSEDSMI 610

BLAST of Chy12G215130 vs. NCBI nr
Match: XP_004146657.1 (MACPF domain-containing protein NSL1 [Cucumis sativus] >KGN64702.1 hypothetical protein Csa_013635 [Cucumis sativus])

HSP 1 Score: 1220 bits (3156), Expect = 0.0
Identity = 608/610 (99.67%), Postives = 609/610 (99.84%), Query Frame = 0

Query: 1   MSFNINRFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA 60
           MSFNINRFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA
Sbjct: 1   MSFNINRFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA 60

Query: 61  GVVVPNVPTSINCDKGERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLKGCW 120
           GVVVPNVPTSINCDKGERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLKGCW
Sbjct: 61  GVVVPNVPTSINCDKGERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLKGCW 120

Query: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHLTLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180
           QNDAGSTRSLAFDGCFITLYNIELEKSH+TLSEQVKREVPSSWDPAALAEFIEKYGTHVV
Sbjct: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180

Query: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGLTSNPVAAKIKDEYS 240
           VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGLTSNPVAAKIKDEYS
Sbjct: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGLTSNPVAAKIKDEYS 240

Query: 241 LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF 300
           LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF
Sbjct: 241 LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF 300

Query: 301 VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK 360
           VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK
Sbjct: 301 VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK 360

Query: 361 QASPSLKFTLMGPKLYVNTTKVDSNNHPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL 420
           QASPSLKFTLMGPKLYVNTTKVDSNN PVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL
Sbjct: 361 QASPSLKFTLMGPKLYVNTTKVDSNNLPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL 420

Query: 421 SDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKGIG 480
           SDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKGIG
Sbjct: 421 SDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKGIG 480

Query: 481 MRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL 540
           MRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL
Sbjct: 481 MRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL 540

Query: 541 NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL 600
           NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL
Sbjct: 541 NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL 600

Query: 601 LTILSEDSMI 610
           LTILSEDSMI
Sbjct: 601 LTILSEDSMI 610

BLAST of Chy12G215130 vs. NCBI nr
Match: TYK26438.1 (MACPF domain-containing protein NSL1-like [Cucumis melo var. makuwa])

HSP 1 Score: 1204 bits (3114), Expect = 0.0
Identity = 601/610 (98.52%), Postives = 604/610 (99.02%), Query Frame = 0

Query: 1   MSFNINRFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA 60
           MSFNINRFNPQSAAEKAVSV+GFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA
Sbjct: 1   MSFNINRFNPQSAAEKAVSVVGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA 60

Query: 61  GVVVPNVPTSINCDKGERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLKGCW 120
           GVVVPNVPTSINCDKGERTRFRSDVISFNQMSEL NQQLSLSGKIPSGFFNTMFGLKGCW
Sbjct: 61  GVVVPNVPTSINCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSGFFNTMFGLKGCW 120

Query: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHLTLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180
           QNDAGSTRSLAFDGCFITLYNIELEKSH+TLSEQVKREVPSSWDPAALAEFIEKYGTHVV
Sbjct: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180

Query: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGLTSNPVAAKIKDEYS 240
           VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGG TSN VAAKIKDEYS
Sbjct: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGFTSNSVAAKIKDEYS 240

Query: 241 LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF 300
           LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF
Sbjct: 241 LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF 300

Query: 301 VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK 360
           VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK
Sbjct: 301 VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK 360

Query: 361 QASPSLKFTLMGPKLYVNTTKVDSNNHPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL 420
           QASPSLKFTLMGPKLYVNTTKVDSNN PVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL
Sbjct: 361 QASPSLKFTLMGPKLYVNTTKVDSNNRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL 420

Query: 421 SDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKGIG 480
           SDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEY+ SLIDDDSASIVTKAWFEVK IG
Sbjct: 421 SDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYHDSLIDDDSASIVTKAWFEVKVIG 480

Query: 481 MRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL 540
           MRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL
Sbjct: 481 MRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL 540

Query: 541 NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL 600
           NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL
Sbjct: 541 NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL 600

Query: 601 LTILSEDSMI 610
           LTILSEDSMI
Sbjct: 601 LTILSEDSMI 610

BLAST of Chy12G215130 vs. NCBI nr
Match: XP_038894492.1 (MACPF domain-containing protein NSL1 [Benincasa hispida])

HSP 1 Score: 1194 bits (3089), Expect = 0.0
Identity = 594/610 (97.38%), Postives = 602/610 (98.69%), Query Frame = 0

Query: 1   MSFNINRFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA 60
           MSFNINRF+PQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTR+RDLVLP 
Sbjct: 1   MSFNINRFDPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRTRDLVLPG 60

Query: 61  GVVVPNVPTSINCDKGERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLKGCW 120
           GVVVPNV TSINCDKGERTRFRSDVISFNQMSEL NQQLSLSGKIPSGFFNTMFGLKGCW
Sbjct: 61  GVVVPNVSTSINCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSGFFNTMFGLKGCW 120

Query: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHLTLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180
           QNDAGSTRSLAFDGCFITLYNIELEKSH+TLSEQVKREVPSSWDPAALAEFIEKYGTHVV
Sbjct: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180

Query: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGLTSNPVAAKIKDEYS 240
           VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLAD+KFSEDANGG TSNP AAKIKDEYS
Sbjct: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADEKFSEDANGGFTSNPAAAKIKDEYS 240

Query: 241 LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF 300
           LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF
Sbjct: 241 LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF 300

Query: 301 VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK 360
           VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK
Sbjct: 301 VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK 360

Query: 361 QASPSLKFTLMGPKLYVNTTKVDSNNHPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL 420
           QASPSLKFTLMGPKLYVNTTKVDS+N PVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL
Sbjct: 361 QASPSLKFTLMGPKLYVNTTKVDSHNRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL 420

Query: 421 SDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKGIG 480
           SDDLSYRPIDEPEEKGYFEPV+WSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVK IG
Sbjct: 421 SDDLSYRPIDEPEEKGYFEPVRWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKVIG 480

Query: 481 MRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL 540
           MRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFS+GLHQPEEQPKIEL
Sbjct: 481 MRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSSGLHQPEEQPKIEL 540

Query: 541 NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL 600
           NSAVYPDGPPLTSRAMKMLN+IDTKEMVRGPENSPGYWVVTGAKLCVEG RISIKVKYSL
Sbjct: 541 NSAVYPDGPPLTSRAMKMLNIIDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKYSL 600

Query: 601 LTILSEDSMI 610
           LTILSEDSMI
Sbjct: 601 LTILSEDSMI 610

BLAST of Chy12G215130 vs. NCBI nr
Match: KAA0057012.1 (MACPF domain-containing protein NSL1 [Cucumis melo var. makuwa])

HSP 1 Score: 1134 bits (2932), Expect = 0.0
Identity = 576/610 (94.43%), Postives = 585/610 (95.90%), Query Frame = 0

Query: 1   MSFNINRFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA 60
           MSFNINRFNPQSAAEKAVSV+GFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA
Sbjct: 1   MSFNINRFNPQSAAEKAVSVVGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA 60

Query: 61  GVVVPNVPTSINCDKGERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLKGCW 120
           GVVVPNVPTSINCDKGERTRFRSDVISFNQMSEL NQQLSLSGKIPSGFFNTMFGLKGCW
Sbjct: 61  GVVVPNVPTSINCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSGFFNTMFGLKGCW 120

Query: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHLTLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180
           QNDAGSTRSLAFDGCFITLYNIELEKSH+TLSEQVKREVPSSWDPAALAEFIEKYGTHVV
Sbjct: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180

Query: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGLTSNPVAAKIKDEYS 240
           VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGG TSN VAAKIK    
Sbjct: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGFTSNSVAAKIK---- 240

Query: 241 LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF 300
           +P +  + + +   P   +H    ELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF
Sbjct: 241 VPTQ-EHKWLSPFGPFHWSH----ELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF 300

Query: 301 VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK 360
           VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK
Sbjct: 301 VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK 360

Query: 361 QASPSLKFTLMGPKLYVNTTKVDSNNHPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL 420
           QASPSLKFTLMGPKLYVNTTKVDSNN PVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL
Sbjct: 361 QASPSLKFTLMGPKLYVNTTKVDSNNRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL 420

Query: 421 SDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKGIG 480
           SDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEY+ SLIDDDSASIVTKAWFEVK IG
Sbjct: 421 SDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYHDSLIDDDSASIVTKAWFEVKVIG 480

Query: 481 MRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL 540
           MRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL
Sbjct: 481 MRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL 540

Query: 541 NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL 600
           NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL
Sbjct: 541 NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL 600

Query: 601 LTILSEDSMI 610
           LTILSEDSMI
Sbjct: 601 LTILSEDSMI 601

BLAST of Chy12G215130 vs. NCBI nr
Match: KAG6573287.1 (MACPF domain-containing protein NSL1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1125 bits (2910), Expect = 0.0
Identity = 553/610 (90.66%), Postives = 584/610 (95.74%), Query Frame = 0

Query: 1   MSFNINRFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA 60
           MSFN+NRF+PQSAAEKAVSVIGFG+DLCNDLRLSSCKPGPSGSKLIDID TR++DLVLPA
Sbjct: 1   MSFNVNRFDPQSAAEKAVSVIGFGFDLCNDLRLSSCKPGPSGSKLIDIDLTRTKDLVLPA 60

Query: 61  GVVVPNVPTSINCDKGERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLKGCW 120
           GVVVPNV  SI CDKGERTRFRSDVISFNQMSEL NQQLSLSGKIPS FFN+MFGLKGCW
Sbjct: 61  GVVVPNVSNSIKCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSAFFNSMFGLKGCW 120

Query: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHLTLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180
           QNDAGS++SL FDGCFITLYNIELE+SH+TLSEQVKREVPSSWDPAAL++FIE YGTHVV
Sbjct: 121 QNDAGSSKSLGFDGCFITLYNIELERSHITLSEQVKREVPSSWDPAALSKFIENYGTHVV 180

Query: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGLTSNPVAAKIKDEYS 240
           VGVKMGGKDVIFMRQLRESN+EQ +VQKKLK+LAD++FSEDANGG  SN  AAKIKDEYS
Sbjct: 181 VGVKMGGKDVIFMRQLRESNIEQAEVQKKLKQLADERFSEDANGGFMSNSTAAKIKDEYS 240

Query: 241 LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF 300
           +PW+LR+AFAASIKPP+I HSKGDELT IY+RRGGVDVGQSHNQWLST+SQSPNVISMSF
Sbjct: 241 MPWQLRHAFAASIKPPIITHSKGDELTTIYVRRGGVDVGQSHNQWLSTVSQSPNVISMSF 300

Query: 301 VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK 360
           VPITSL+NGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLA RHRK
Sbjct: 301 VPITSLLNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLATRHRK 360

Query: 361 QASPSLKFTLMGPKLYVNTTKVDSNNHPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL 420
           QASPSLKFTLMGPKLYVNTTKVDSN+ PVTGVRLFLEGKKSDHLAIHLQHLSTLPKT+QL
Sbjct: 361 QASPSLKFTLMGPKLYVNTTKVDSNSRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTIQL 420

Query: 421 SDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKGIG 480
           SDDLSY PIDEPEEKGYF+PVKWSIFSHVCTAPV+YNGSLIDDD+ASIVTKAWFEVK IG
Sbjct: 421 SDDLSYGPIDEPEEKGYFQPVKWSIFSHVCTAPVQYNGSLIDDDTASIVTKAWFEVKVIG 480

Query: 481 MRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL 540
           MRKVLFLRLGFS VASA IRRS+WEGPST+SRKSGLISTFISTRFS GL QPE QPKIEL
Sbjct: 481 MRKVLFLRLGFSTVASAKIRRSEWEGPSTSSRKSGLISTFISTRFSTGLQQPEAQPKIEL 540

Query: 541 NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL 600
           NSAVYPDGPPLT RAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEG RISIKVKYSL
Sbjct: 541 NSAVYPDGPPLTVRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKYSL 600

Query: 601 LTILSEDSMI 610
           LTILSEDSMI
Sbjct: 601 LTILSEDSMI 610

BLAST of Chy12G215130 vs. TAIR 10
Match: AT1G28380.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 789.6 bits (2038), Expect = 1.7e-228
Identity = 391/612 (63.89%), Postives = 474/612 (77.45%), Query Frame = 0

Query: 4   NINRFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPAGVV 63
           N  R +  SAAEKAVSVIG GYDLC+D+R S+CK  P GS+L++ID TR+RDL+ P G+V
Sbjct: 5   NFTRLDAHSAAEKAVSVIGLGYDLCSDVRFSACKTTPDGSRLVEIDPTRNRDLIFPGGIV 64

Query: 64  VPNVPTSINCDKGERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLKGCWQND 123
           V NV +SI CDKGERTR RSD++SFNQMSE  NQ + LSGKIPSG FN MF    CW  D
Sbjct: 65  VNNVSSSIKCDKGERTRLRSDILSFNQMSEKFNQDMCLSGKIPSGMFNNMFAFSKCWPKD 124

Query: 124 AGSTRSLAFDGCFITLYNIELEKSHLTLSEQVKREVPSSWDPAALAEFIEKYGTHVVVGV 183
           A S ++LA+DG FI+LY++E+ +  LTL ++VKREVPSSWD AALA FIEKYGTHVVVGV
Sbjct: 125 ASSVKTLAYDGWFISLYSVEIVRKQLTLRDEVKREVPSSWDSAALAGFIEKYGTHVVVGV 184

Query: 184 KMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSED-ANGGLTSNPVAAKIKDEYSLP 243
            MGGKDVI ++Q+R+SN E  ++QK LK   D++F  D       ++  + K K+E  L 
Sbjct: 185 TMGGKDVIHVKQMRKSNHEPEEIQKMLKHWGDERFCVDPVESKSPASVYSGKPKEENLLQ 244

Query: 244 WELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSFVP 303
           W L+  F  S+   V+ H+K +E+  + IRRGGVD+GQSH +WLST+SQ+PNVISM FVP
Sbjct: 245 WGLQ-PFGTSVSSAVVMHTKNEEIMRVCIRRGGVDLGQSHERWLSTVSQAPNVISMCFVP 304

Query: 304 ITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRKQA 363
           ITSL++G+ G GFLSHAVNLYLRYKPPIEEL QFLEFQLPRQWAPVY DLPL  R  KQ+
Sbjct: 305 ITSLLSGLPGTGFLSHAVNLYLRYKPPIEELHQFLEFQLPRQWAPVYGDLPLGLRRSKQS 364

Query: 364 SPSLKFTLMGPKLYVNTTKVDSNNHPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQLSD 423
           SPSL+F+LMGPKLYVNT+KVDS   PVTG+R FLEGKK +HLAIHLQHLS  P +L LS 
Sbjct: 365 SPSLQFSLMGPKLYVNTSKVDSGERPVTGLRFFLEGKKGNHLAIHLQHLSACPPSLHLSH 424

Query: 424 DLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKGIGMR 483
           D +Y PI+EP EKGY+ PVKW IFSHVCT PV+YNG+   DD+ASIVTKAW EVKG+GMR
Sbjct: 425 DDTYEPIEEPVEKGYYVPVKWGIFSHVCTYPVQYNGAR-SDDTASIVTKAWLEVKGMGMR 484

Query: 484 KVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLH----QPEEQPKI 543
           KVLFLRLGFS+ ASA  R+S W+  ST SRKSG+ S  ISTR S GL       + Q KI
Sbjct: 485 KVLFLRLGFSLDASAVTRKSCWDNLSTNSRKSGVFS-MISTRLSTGLSPNPATTKPQSKI 544

Query: 544 ELNSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKY 603
           ++NSAVYP GP    +  K+L+++DTKE++RGPE  PGYWVVTGAKLCVE G+ISIK KY
Sbjct: 545 DINSAVYPRGPSPPVKP-KLLSLVDTKEVMRGPEEQPGYWVVTGAKLCVEAGKISIKAKY 604

Query: 604 SLLTILSEDSMI 611
           SLLT++SEDS++
Sbjct: 605 SLLTVISEDSLV 612

BLAST of Chy12G215130 vs. TAIR 10
Match: AT4G24290.2 (MAC/Perforin domain-containing protein )

HSP 1 Score: 626.3 bits (1614), Expect = 2.5e-179
Identity = 318/610 (52.13%), Postives = 419/610 (68.69%), Query Frame = 0

Query: 7   RFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDI-DFTRSRDLVLPAGVVVP 66
           R     AAE A+  IG GYDL  DLRL  CK G   S+L+DI +   + ++VLP G+ +P
Sbjct: 4   RLPASKAAEVAIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGISIP 63

Query: 67  NVPTSINCDKGERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLKGCWQNDAG 126
           NV  SI CDKGER RFRSD++ F QM+E  NQ+LSL+GKIPSG FN MF    CWQ DA 
Sbjct: 64  NVSKSIKCDKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQKDAA 123

Query: 127 STRSLAFDGCFITLYNIELEKSHLTLSEQVKREVPSSWDPAALAEFIEKYGTHVVVGVKM 186
            T++LAFDG FI+LY++ L+KS + L E VK+ VPS+WDPAALA FI+ YGTH++V VKM
Sbjct: 124 YTKNLAFDGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKM 183

Query: 187 GGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDA---NGGLTSNPVAAKIKDEYSLP 246
           GGKDVI+ +Q   S ++  D+QK+LK++AD +F E +   N G      ++K++ +    
Sbjct: 184 GGKDVIYAKQQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKEQ-- 243

Query: 247 WELRNAFAASIKPPVINHSKGDELTAIYIRRGGVD-VGQSHNQWLSTISQSPNVISMSFV 306
             LR A  +S+     +++  ++   +  RRGG D     HN+WL T+   P+VISMSF+
Sbjct: 244 -RLRFADTSSLG----SYANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFI 303

Query: 307 PITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRKQ 366
           PITSL+NGV G+GFLSHA+NLYLRYKPPIEEL QFLEFQLPRQWAPV+++LPL P+ ++Q
Sbjct: 304 PITSLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQRKQQ 363

Query: 367 ASPSLKFTLMGPKLYVNTTKVDSNNHPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQLS 426
           +  SL+F+  GPKLYVNTT VD    P+TG+RL+LEG++S+ LAIHLQHLS+LPK  QL 
Sbjct: 364 SCASLQFSFFGPKLYVNTTPVDVGKRPITGMRLYLEGRRSNRLAIHLQHLSSLPKIYQLE 423

Query: 427 DDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKGIGM 486
           DDL+     E  ++ Y+E V W  +SHVCT PVE       DD  S+VT A   V+  G 
Sbjct: 424 DDLNRSIRQESHDRRYYEKVNWKNYSHVCTEPVE------SDDDLSVVTGAQLHVESHGF 483

Query: 487 RKVLFLRLGFSMVASAG-IRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL 546
           + VLFLRL FS V  A  ++ S+W+     + KSGLIST IS  F+A    P     + +
Sbjct: 484 KNVLFLRLCFSRVVGATLVKNSEWDEAVGFAPKSGLISTLISHHFTAAQKPPPRPADVNI 543

Query: 547 NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL 606
           NSA+YP GPP+ ++A K+L  +DT EM RGP+ SPGYWVV+GA+L VE G+IS+KVKYSL
Sbjct: 544 NSAIYPGGPPVPTQAPKLLKFVDTSEMTRGPQESPGYWVVSGARLLVEKGKISLKVKYSL 600

Query: 607 LTILSEDSMI 611
            T +  D +I
Sbjct: 604 FTPILGDEVI 600

BLAST of Chy12G215130 vs. TAIR 10
Match: AT1G14780.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 466.1 bits (1198), Expect = 4.4e-131
Identity = 258/614 (42.02%), Postives = 377/614 (61.40%), Query Frame = 0

Query: 15  EKAVSVIGFGYDLCNDLRLSSCKPGPSGS---KLIDIDFTRSRDLVLPAGVVVPNVPTSI 74
           E AV  +G G+DL  D RL  CK G   +   +L+ +D T++R+L +P   V  NV   I
Sbjct: 10  ETAVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFGVFQNVSADI 69

Query: 75  NCDKGERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLK-GCWQNDAGSTRSL 134
           NCDKGERTRFRSD++ FN+MSE  NQ+ S++GKIPSG FN  FG + G W  DA + +SL
Sbjct: 70  NCDKGERTRFRSDILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWATDAANVKSL 129

Query: 135 AFDGCFITLYNIELEK-SHLTLSEQVKREVPSSWDPAALAEFIEKYGTHVVVGVKMGGKD 194
             D   +TL+N+ +   + L L+++V+  VPSSWDP  LA FIE+YGTHV+ GV +GG+D
Sbjct: 130 GLDASVVTLFNLHIHNPNRLRLTDRVRNAVPSSWDPQLLARFIERYGTHVITGVSVGGQD 189

Query: 195 VIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGLTSNPVAAKIKDEYSLPW--ELRN 254
           V+ +RQ + S+++   ++  L  L D  F+      L++  +       +S P   E  N
Sbjct: 190 VVVVRQDKSSDLDNDLLRHHLYDLGDQLFTGSCL--LSTRRLNKAYHHSHSQPKFPEAFN 249

Query: 255 AF----AASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSFVPI 314
            F      +     IN   G  +T I  +RGG    +SH++WL T+   P+ I+ +F+PI
Sbjct: 250 VFDDKQTVAFNNFSINSQNG--ITVICAKRGGDGRAKSHSEWLITVPDKPDAINFNFIPI 309

Query: 315 TSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPL-APRHRKQA 374
           TSL+  V G+G LSHA++LYLRYKPP+ +LQ FL+F  PR WAPV+ DLP  A  +   A
Sbjct: 310 TSLLKDVPGSGLLSHAMSLYLRYKPPLMDLQYFLDFSGPRAWAPVHNDLPFGAAPNMASA 369

Query: 375 SPSLKFTLMGPKLYVNTTKVDSNNHPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTL--QL 434
            P+L    MGPKLYVNTT V S  +PVTG+R FLEGKK + LAIHLQHL     T+  ++
Sbjct: 370 YPALHINFMGPKLYVNTTPVTSEKNPVTGMRFFLEGKKCNRLAIHLQHLDNTRTTVGEKI 429

Query: 435 SDDLSYRPIDE-PEEKGYFEPVKWSIFSHVCTAPVEYNGSLI--------DDDSASIVTK 494
           +D+  +R  D+  +   YFEP+    FSHVCT PV+Y+ + I         +D A IVT 
Sbjct: 430 TDEHIWRGSDQITDNDRYFEPLNGKKFSHVCTVPVKYDPNWIKTTSNHKSQNDVAFIVTG 489

Query: 495 AWFEVKGIGMRKVLFLRLGFSMVASAGIRRSDW-EGPSTTSRKSGLISTFISTRFSAGLH 554
           A  EVK  G + VL LRL ++ V+   + ++ W  GP  TS+KSG+ S+      S  +H
Sbjct: 490 AQLEVKKHGSKSVLHLRLRYTKVSDHYVVQNSWVHGPIGTSQKSGIFSSMSMPLTSGSVH 549

Query: 555 Q---PEEQPKIELNSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCV 602
                +++ ++ L+S V+P GPP+ +   K++  +D  ++ RGP++SPG+W+VTG +L +
Sbjct: 550 HNMIQKDKNEVVLDSGVFPGGPPVPAN-NKIVKFVDLSQLCRGPQHSPGHWLVTGVRLYL 609

BLAST of Chy12G215130 vs. TAIR 10
Match: AT1G29690.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 426.4 bits (1095), Expect = 3.9e-119
Identity = 239/600 (39.83%), Postives = 348/600 (58.00%), Query Frame = 0

Query: 17  AVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPAGVVVPNVPTSINCDKG 76
           A+  +G G+D+ +D+RL  CK  P GS+L+ I+  ++RDL L  G ++PNVP  I+C +G
Sbjct: 24  AIQALGRGFDVTSDVRLLYCKGAP-GSRLVRIEEGQNRDLELSHGFLLPNVPADIDCSRG 83

Query: 77  ERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLKGCWQNDAGSTRSLAFDGCF 136
                R  V SF++M+E  N +  + G IP G FN MF   G WQ DA ST+SLA  G F
Sbjct: 84  NSGTQRISVCSFHEMAEEFNVRSGVKGNIPLGCFNAMFNYTGSWQVDAASTKSLALVGYF 143

Query: 137 ITLYNIELEKSHLTLSEQVKREVPSSWDPAALAEFIEKYGTHVVVGVKMGGKDVIFMRQL 196
           I LY+++L K  L L  +++R VPSSWDPA+LA FIE YGTH+V  V +GG+DV+++RQ 
Sbjct: 144 IPLYDVKLAKLTLVLHNEIRRAVPSSWDPASLASFIENYGTHIVTSVTIGGRDVVYIRQH 203

Query: 197 RESNVEQTDVQKKLKKLADDKFSEDANGGLTSNPVAAKIKDEYSLPWELRNAFAASIKPP 256
           + S +  ++++  +  +   +F E  +  +T  P+  K KD                   
Sbjct: 204 QSSPLPVSEIENYVNDMIKHRFHEAESQSIT-GPLKYKDKD------------------- 263

Query: 257 VINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSFVPITSLMNGVRGNGFL 316
                    +T I+ RRGG D+ QSH +W  T+  +P++I+M+F PI SL+ GV G   L
Sbjct: 264 ---------ITVIFRRRGGDDLEQSHARWAETVPAAPDIINMTFTPIVSLLEGVPGLRHL 323

Query: 317 SHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRKQASPSLKFTLMGPKLY 376
           + A+ LYL YKPPIE+LQ FL++Q+ R WAP  ++L    + ++    SL+F+LMGPKL+
Sbjct: 324 TRAIELYLEYKPPIEDLQYFLDYQIARAWAPEQSNL----QRKEPVCSSLQFSLMGPKLF 383

Query: 377 VNTTKVDSNNHPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQLSDDLSYRPIDEPEEKG 436
           ++  +V     PVTG+RL LEG K + L+IHLQHL +LPK LQ   D S+ PI  P+ +G
Sbjct: 384 ISADQVTVGRKPVTGLRLSLEGSKQNRLSIHLQHLVSLPKILQPHWD-SHVPIGAPKWQG 443

Query: 437 -------YFEPVKWSIFSHVCTAPVEYNGSLIDD-DSASIVTKAWFEVKGIGMRKVLFLR 496
                  +FEP+KW  FSHV T+P+E+  + I D     IVT A   V   G + VL L+
Sbjct: 444 PEEQDSRWFEPIKWKNFSHVSTSPIEHTETHIGDLSGVHIVTGAQLGVWDFGSKNVLHLK 503

Query: 497 LGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIELNSAVYPDG 556
           L FS V    IRRS W+                        H P     +  +  + P G
Sbjct: 504 LLFSKVPGCTIRRSVWD------------------------HTP-----VASSGRLEPGG 559

Query: 557 PPLTS-------RAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSLL 602
           P  +S       ++ K+  ++D+ EM++GP++ PG+W+VTGAKL VE G+I ++VKYSLL
Sbjct: 564 PSTSSSTEEVSGQSGKLAKIVDSSEMLKGPQDLPGHWLVTGAKLGVEKGKIVLRVKYSLL 559

BLAST of Chy12G215130 vs. TAIR 10
Match: AT4G24290.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 322.0 bits (824), Expect = 1.0e-87
Identity = 166/327 (50.76%), Postives = 222/327 (67.89%), Query Frame = 0

Query: 7   RFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDI-DFTRSRDLVLPAGVVVP 66
           R     AAE A+  IG GYDL  DLRL  CK G   S+L+DI +   + ++VLP G+ +P
Sbjct: 4   RLPASKAAEVAIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGISIP 63

Query: 67  NVPTSINCDKGERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLKGCWQNDAG 126
           NV  SI CDKGER RFRSD++ F QM+E  NQ+LSL+GKIPSG FN MF    CWQ DA 
Sbjct: 64  NVSKSIKCDKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQKDAA 123

Query: 127 STRSLAFDGCFITLYNIELEKSHLTLSEQVKREVPSSWDPAALAEFIEKYGTHVVVGVKM 186
            T++LAFDG FI+LY++ L+KS + L E VK+ VPS+WDPAALA FI+ YGTH++V VKM
Sbjct: 124 YTKNLAFDGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKM 183

Query: 187 GGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDA---NGGLTSNPVAAKIKDEYSLP 246
           GGKDVI+ +Q   S ++  D+QK+LK++AD +F E +   N G      ++K++ +    
Sbjct: 184 GGKDVIYAKQQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKEQ-- 243

Query: 247 WELRNAFAASIKPPVINHSKGDELTAIYIRRGGVD-VGQSHNQWLSTISQSPNVISMSFV 306
             LR A  +S+     +++  ++   +  RRGG D     HN+WL T+   P+VISMSF+
Sbjct: 244 -RLRFADTSSLG----SYANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFI 303

Query: 307 PITSLMNGVRGNGFLSHAVNLYLRYKP 329
           PITSL+NGV G+GFLSHA+NLYLR KP
Sbjct: 304 PITSLLNGVPGSGFLSHAINLYLRCKP 323