Chy12G214910 (gene) Cucumber (hystrix) v1

Overview
NameChy12G214910
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionProtein kinase domain-containing protein
LocationchrH12: 14274954 .. 14277604 (-)
RNA-Seq ExpressionChy12G214910
SyntenyChy12G214910
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTTGTTTTCGTTGTACCGGTTCATCCAGCAAGAAATCGGAGGATATCGAACAAAAAGACCCCAATACTTTCAATTCCAAAACCCATAAGCCCGATGATCGAACCCCTTCAGGTTATTTTTTTATTATCTTTTCAACAATATCTTAAATGGGTCAAATCGATTCTGTTTGACCTTCTTGATTTTGGGTTTTCTTTTTCTTTGAAATTTTTTTGTCATGGGATTTCTGCGAATTAATTCTTTTTTTTTTTTTCTGGTTGAGGAATTAGGAATTGAGATTGGTTTTCTGTTTGGGGTTTAATGTAATGTTTGGACTTCCAATCTGTTTGTATTTTCTGCTAATTAGTTGCAAGGTTTTATAAGCTATTTGAGAGGAAAAATCTCATGTCAAGAAAATTTTAATGTAATTTGTGATGTTTTTTTCTCTTTGGTTTGAATTTCAGGTAGTTGTTTGGTGGTGGTTTACTGGTTTTTGGATTATGGTCAAGTGAATTAAGTAAAGAGTTTTGTTTCTTATTGTTTTTTAAATTTTCTTTTGGGTTTGTTCAGATAAAGCCAAAGTTATTTCAAATTTGGTGAAGAGGGATTCTGATGTGAAGGTGGTTGTGTCTAAAAATGACCAATTGGCTTTAGATGTAAAGAAATTGAATTTGAAAGATGAGGTTTCTGAGGAAGGAGAAGCCAATGGCCGTGCTAAGACGTTTACATTTGAAGAACTTGCAGCCGCGACGGGCAATTTCAGGTCAGATTGCTTCTTGGGTGAAGGAGGATTTGGCAAAGTTTACAAGGGGTACTTGGAAAAAGTTAATGAGGTACATTCAGTGAAACATTTTACTGTTTATGTGTAGGGTCATCATGCATTTTCTAAGGCTTTTATTTGTAAATGTAAGACAAAATTCAAACTGAAAACAATTATAAACCAAAACCGTTCTCATGTGCTAATAGTATTAGGCTGAATTGCAAGTTTGGTCATATCTATGATTTGGAGAAAGTTGGGATTTAATTCCTATGGTTTGATAAAACTCTCCTAAAGGGTCCTACTGTATGACTATTAAAGAGGTTTTTGTCAAACCACAAGGACTAATGTCTAACTTCTTTCAACCATAGGGACCAAAATTTGAAATTTAACCTAGTATTAAGCAAGCTAGATGTTCATATAGTATGGTACTGTTTCTCATTATCTCTCTTTCTTTAATAGAATTTGTGCTTCTGTTTAATTTGTTAAGTTCCATTAAATAGCAATGCTTTAGTTATCTAACCCCTTCTTTTGCTTTGGTCTAGGTTGTAGCAATTAAACAGCTTGACCGCAATGGACTTCAAGGAATTCGAGAGTTTGTTGTGGAAGTTTTGACTTTAAGTCTAGCTGATCATCCAAACCTTGTAAAACTTATTGGCTTTTGTGCTGAAGGGGATCAGAGATTATTGGTTTATGAATATATGCCATTGGGATCATTGGAAAACCATTTACACGGTACCTAATTAATTGACCCACGGCTTATAATCCTTTTGCCTACAATGTTTCTCGATTATTTTACATATTCTTCGCTCCTTGTAGATCTTCGACCTGGTGCCAAAGTAATTGATTGGAACACAAGAATGAAAATAGCAGCAGGTGCAGCGAGGGGGTTGGAGTATTTGCATGAGAAGATGAAGCCCCCGGTTATATATCGTGATTTGAAATGCTCAAACATTTTACTCGGTGAAGGATATCATCCAAAGCTATCTGATTTTGGATTGGCTAAAGTAGGTCCTAGTGGGGATAAAACCCACGTTTCAACCAGGGTTATGGGCACATATGGGTATTGCGCTCCAGATTATGCAATGACAGGCCAGCTGACATTCAAATCAGATATTTACAGCTTTGGCGTTGTTTTATTGGAACTCATCACGGGCAGGAAAGCAATCGATCATTCAAAACCTCATTCGGAGCAAAATCTTGTGGCTTGGGTAATATAGCTAGCCTATTCTCTTTTGTAGTCTAGTTCCACTTTTATGATTGTATAGTTGTTTCTTCATGAAAATGCATGCTTTGGAAATATTATTTGTAGAACTTAAACATTTGACTGCTCGGCATAATACTTTGATGGTTTGTGCATGTACTTGAGCGGATTGTTTCAAACATTTGGAAACTTTGTTTGTCGTAACAGGTGGAGTTTAGTGATATAAACCATTTGAATTACGTTAGACTTTTAGTAGGCTGCTACATGCAAAACAAATTCATTGCATGATAAGAAAATGCTGGGTGTATTTATTTGCATATTTTGAGTTCTGTGAAGCGCACAAACATTGGTTCTGAATGCCCATTGGAGGCTAAAGCAAACTTATGATGTGTCTGCTATTGCAGGCGCGACCCTTGTTCCGAGACCGAAAGAAGTTCTCACAAATGGTTGATCCAATGCTCCAAGGACATTATCCTGTGAGAGGATTATACCAATCTCTAGCCATAGCCGCCATGTGTGTCCAGGAGCAACCGAATATGCGACCAGTCATAACTGATGTTGTAACAGCTTTAAACTACCTAGCGTCCCAGAAATACGATCCCCAGACTCAACCAAACCAAAACTCCCAAAAGAGACCATCTTCTCATAAAATGGAACGGAGTGATGATAAGAATGAAACTTCAGACAACGATGAGTCAGAGAGAGAGTGA

mRNA sequence

ATGGGTTGTTTTCGTTGTACCGGTTCATCCAGCAAGAAATCGGAGGATATCGAACAAAAAGACCCCAATACTTTCAATTCCAAAACCCATAAGCCCGATGATCGAACCCCTTCAGATAAAGCCAAAGTTATTTCAAATTTGGTGAAGAGGGATTCTGATGTGAAGGTGGTTGTGTCTAAAAATGACCAATTGGCTTTAGATGTAAAGAAATTGAATTTGAAAGATGAGGTTTCTGAGGAAGGAGAAGCCAATGGCCGTGCTAAGACGTTTACATTTGAAGAACTTGCAGCCGCGACGGGCAATTTCAGGTCAGATTGCTTCTTGGGTGAAGGAGGATTTGGCAAAGTTTACAAGGGGTACTTGGAAAAAGTTAATGAGGTTGTAGCAATTAAACAGCTTGACCGCAATGGACTTCAAGGAATTCGAGAGTTTGTTGTGGAAGTTTTGACTTTAAGTCTAGCTGATCATCCAAACCTTGTAAAACTTATTGGCTTTTGTGCTGAAGGGGATCAGAGATTATTGGTTTATGAATATATGCCATTGGGATCATTGGAAAACCATTTACACGATCTTCGACCTGGTGCCAAAGTAATTGATTGGAACACAAGAATGAAAATAGCAGCAGGTGCAGCGAGGGGGTTGGAGTATTTGCATGAGAAGATGAAGCCCCCGGTTATATATCGTGATTTGAAATGCTCAAACATTTTACTCGGTGAAGGATATCATCCAAAGCTATCTGATTTTGGATTGGCTAAAGTAGGTCCTAGTGGGGATAAAACCCACGTTTCAACCAGGGTTATGGGCACATATGGGTATTGCGCTCCAGATTATGCAATGACAGGCCAGCTGACATTCAAATCAGATATTTACAGCTTTGGCGTTGTTTTATTGGAACTCATCACGGGCAGGAAAGCAATCGATCATTCAAAACCTCATTCGGAGCAAAATCTTGTGGCTTGGGCGCGACCCTTGTTCCGAGACCGAAAGAAGTTCTCACAAATGGTTGATCCAATGCTCCAAGGACATTATCCTGTGAGAGGATTATACCAATCTCTAGCCATAGCCGCCATGTGTGTCCAGGAGCAACCGAATATGCGACCAGTCATAACTGATGTTGTAACAGCTTTAAACTACCTAGCGTCCCAGAAATACGATCCCCAGACTCAACCAAACCAAAACTCCCAAAAGAGACCATCTTCTCATAAAATGGAACGGAGTGATGATAAGAATGAAACTTCAGACAACGATGAGTCAGAGAGAGAGTGA

Coding sequence (CDS)

ATGGGTTGTTTTCGTTGTACCGGTTCATCCAGCAAGAAATCGGAGGATATCGAACAAAAAGACCCCAATACTTTCAATTCCAAAACCCATAAGCCCGATGATCGAACCCCTTCAGATAAAGCCAAAGTTATTTCAAATTTGGTGAAGAGGGATTCTGATGTGAAGGTGGTTGTGTCTAAAAATGACCAATTGGCTTTAGATGTAAAGAAATTGAATTTGAAAGATGAGGTTTCTGAGGAAGGAGAAGCCAATGGCCGTGCTAAGACGTTTACATTTGAAGAACTTGCAGCCGCGACGGGCAATTTCAGGTCAGATTGCTTCTTGGGTGAAGGAGGATTTGGCAAAGTTTACAAGGGGTACTTGGAAAAAGTTAATGAGGTTGTAGCAATTAAACAGCTTGACCGCAATGGACTTCAAGGAATTCGAGAGTTTGTTGTGGAAGTTTTGACTTTAAGTCTAGCTGATCATCCAAACCTTGTAAAACTTATTGGCTTTTGTGCTGAAGGGGATCAGAGATTATTGGTTTATGAATATATGCCATTGGGATCATTGGAAAACCATTTACACGATCTTCGACCTGGTGCCAAAGTAATTGATTGGAACACAAGAATGAAAATAGCAGCAGGTGCAGCGAGGGGGTTGGAGTATTTGCATGAGAAGATGAAGCCCCCGGTTATATATCGTGATTTGAAATGCTCAAACATTTTACTCGGTGAAGGATATCATCCAAAGCTATCTGATTTTGGATTGGCTAAAGTAGGTCCTAGTGGGGATAAAACCCACGTTTCAACCAGGGTTATGGGCACATATGGGTATTGCGCTCCAGATTATGCAATGACAGGCCAGCTGACATTCAAATCAGATATTTACAGCTTTGGCGTTGTTTTATTGGAACTCATCACGGGCAGGAAAGCAATCGATCATTCAAAACCTCATTCGGAGCAAAATCTTGTGGCTTGGGCGCGACCCTTGTTCCGAGACCGAAAGAAGTTCTCACAAATGGTTGATCCAATGCTCCAAGGACATTATCCTGTGAGAGGATTATACCAATCTCTAGCCATAGCCGCCATGTGTGTCCAGGAGCAACCGAATATGCGACCAGTCATAACTGATGTTGTAACAGCTTTAAACTACCTAGCGTCCCAGAAATACGATCCCCAGACTCAACCAAACCAAAACTCCCAAAAGAGACCATCTTCTCATAAAATGGAACGGAGTGATGATAAGAATGAAACTTCAGACAACGATGAGTCAGAGAGAGAGTGA

Protein sequence

MGCFRCTGSSSKKSEDIEQKDPNTFNSKTHKPDDRTPSDKAKVISNLVKRDSDVKVVVSKNDQLALDVKKLNLKDEVSEEGEANGRAKTFTFEELAAATGNFRSDCFLGEGGFGKVYKGYLEKVNEVVAIKQLDRNGLQGIREFVVEVLTLSLADHPNLVKLIGFCAEGDQRLLVYEYMPLGSLENHLHDLRPGAKVIDWNTRMKIAAGAARGLEYLHEKMKPPVIYRDLKCSNILLGEGYHPKLSDFGLAKVGPSGDKTHVSTRVMGTYGYCAPDYAMTGQLTFKSDIYSFGVVLLELITGRKAIDHSKPHSEQNLVAWARPLFRDRKKFSQMVDPMLQGHYPVRGLYQSLAIAAMCVQEQPNMRPVITDVVTALNYLASQKYDPQTQPNQNSQKRPSSHKMERSDDKNETSDNDESERE*
Homology
BLAST of Chy12G214910 vs. ExPASy Swiss-Prot
Match: Q9LQQ8 (Probable serine/threonine-protein kinase PBL5 OS=Arabidopsis thaliana OX=3702 GN=PBL5 PE=2 SV=1)

HSP 1 Score: 568.5 bits (1464), Expect = 6.1e-161
Identity = 302/429 (70.40%), Postives = 349/429 (81.35%), Query Frame = 0

Query: 1   MGCFRCTGSSSKKSEDIEQKDPN-------TFNSKTHKPDDRT-PSDKAKVISNLVKRDS 60
           MGCF C+  SSK+SE  +    N       T  +K+ K DD+T PS  +  +S    RD 
Sbjct: 1   MGCFGCSKKSSKRSETNKDTVINRKIVGGTTSVAKSDKRDDQTQPSSDSTKVSPY--RDV 60

Query: 61  DVKVVVSKNDQLALDVKKLNLKDEVSEEGEANGRAKTFTFEELAAATGNFRSDCFLGEGG 120
           + +  V K DQL+LDVK LNL D+V+ +     +A+TFTF+ELA ATGNFRSDCFLGEGG
Sbjct: 61  NNEGGVGKEDQLSLDVKGLNLNDQVTGK-----KAQTFTFQELAEATGNFRSDCFLGEGG 120

Query: 121 FGKVYKGYLEKVNEVVAIKQLDRNGLQGIREFVVEVLTLSLADHPNLVKLIGFCAEGDQR 180
           FGKV+KG +EK+++VVAIKQLDRNG+QGIREFVVEVLTLSLADHPNLVKLIGFCAEGDQR
Sbjct: 121 FGKVFKGTIEKLDQVVAIKQLDRNGVQGIREFVVEVLTLSLADHPNLVKLIGFCAEGDQR 180

Query: 181 LLVYEYMPLGSLENHLHDLRPGAKVIDWNTRMKIAAGAARGLEYLHEKMKPPVIYRDLKC 240
           LLVYEYMP GSLE+HLH L  G K +DWNTRMKIAAGAARGLEYLH++M PPVIYRDLKC
Sbjct: 181 LLVYEYMPQGSLEDHLHVLPSGKKPLDWNTRMKIAAGAARGLEYLHDRMTPPVIYRDLKC 240

Query: 241 SNILLGEGYHPKLSDFGLAKVGPSGDKTHVSTRVMGTYGYCAPDYAMTGQLTFKSDIYSF 300
           SNILLGE Y PKLSDFGLAKVGPSGDKTHVSTRVMGTYGYCAPDYAMTGQLTFKSDIYSF
Sbjct: 241 SNILLGEDYQPKLSDFGLAKVGPSGDKTHVSTRVMGTYGYCAPDYAMTGQLTFKSDIYSF 300

Query: 301 GVVLLELITGRKAIDHSKPHSEQNLVAWARPLFRDRKKFSQMVDPMLQGHYPVRGLYQSL 360
           GVVLLELITGRKAID++K   +QNLV WARPLF+DR+ F +MVDP+LQG YPVRGLYQ+L
Sbjct: 301 GVVLLELITGRKAIDNTKTRKDQNLVGWARPLFKDRRNFPKMVDPLLQGQYPVRGLYQAL 360

Query: 361 AIAAMCVQEQPNMRPVITDVVTALNYLASQKYDPQTQPNQNSQKRPSSHKMERSDDKNET 420
           AI+AMCVQEQP MRPV++DVV ALN+LAS KYDP + P+ +S K PS H+ +R D++   
Sbjct: 361 AISAMCVQEQPTMRPVVSDVVLALNFLASSKYDPNS-PSSSSGKNPSFHR-DRDDEEKRP 420

Query: 421 SDNDESERE 422
               E+E E
Sbjct: 421 HLVKETECE 420

BLAST of Chy12G214910 vs. ExPASy Swiss-Prot
Match: Q9SIB6 (Probable serine/threonine-protein kinase PBL6 OS=Arabidopsis thaliana OX=3702 GN=PBL6 PE=2 SV=1)

HSP 1 Score: 536.6 bits (1381), Expect = 2.6e-151
Identity = 283/429 (65.97%), Postives = 335/429 (78.09%), Query Frame = 0

Query: 1   MGCFRCTGSSSKKSEDIEQKDPNTFNSKTHKPDDRTPSDKAKVISN---LVKRDSDV--- 60
           MGCF  T  S+K+S+           +KT K +D TP  K  V +N   L +  SD    
Sbjct: 1   MGCFGRTPKSNKRSD-----------TKTTKNNDFTPK-KLTVNANRDKLTQPSSDCLKV 60

Query: 61  -------KVVVSKNDQLALDVKKLNLKDEVSEEGEANGRAKTFTFEELAAATGNFRSDCF 120
                  K +V+K DQLALD K  N++DEV  +     +A+TFTFEEL+ +TGNF+SDCF
Sbjct: 61  SICGDVSKEIVTKKDQLALDAKDTNVEDEVIVK-----KAQTFTFEELSVSTGNFKSDCF 120

Query: 121 LGEGGFGKVYKGYLEKVNEVVAIKQLDRNGLQGIREFVVEVLTLSLADHPNLVKLIGFCA 180
           LGEGGFGKVYKG++EK+N+VVAIKQLDRNG QGIREFVVEVLTLSLADHPNLVKLIGFCA
Sbjct: 121 LGEGGFGKVYKGFIEKINQVVAIKQLDRNGAQGIREFVVEVLTLSLADHPNLVKLIGFCA 180

Query: 181 EGDQRLLVYEYMPLGSLENHLHDLRPGAKVIDWNTRMKIAAGAARGLEYLHEKMKPPVIY 240
           EG QRLLVYEYMPLGSL+NHLHDL  G   + WNTRMKIAAGAARGLEYLH+ MKPPVIY
Sbjct: 181 EGVQRLLVYEYMPLGSLDNHLHDLPSGKNPLAWNTRMKIAAGAARGLEYLHDTMKPPVIY 240

Query: 241 RDLKCSNILLGEGYHPKLSDFGLAKVGPSGDKTHVSTRVMGTYGYCAPDYAMTGQLTFKS 300
           RDLKCSNIL+ EGYH KLSDFGLAKVGP G +THVSTRVMGTYGYCAPDYA+TGQLTFKS
Sbjct: 241 RDLKCSNILIDEGYHAKLSDFGLAKVGPRGSETHVSTRVMGTYGYCAPDYALTGQLTFKS 300

Query: 301 DIYSFGVVLLELITGRKAIDHSKPHSEQNLVAWARPLFRDRKKFSQMVDPMLQGHYPVRG 360
           D+YSFGVVLLELITGRKA D+++  + Q+LV WA PLF+DRK F +MVDP+L+G YPVRG
Sbjct: 301 DVYSFGVVLLELITGRKAYDNTRTRNHQSLVEWANPLFKDRKNFKKMVDPLLEGDYPVRG 360

Query: 361 LYQSLAIAAMCVQEQPNMRPVITDVVTALNYLASQKYDPQTQPNQNSQKRPSSHKMERSD 417
           LYQ+LAIAAMCVQEQP+MRPVI DVV AL++LAS KYD       + QK+ +  + +  +
Sbjct: 361 LYQALAIAAMCVQEQPSMRPVIADVVMALDHLASSKYD-----RSHRQKQDNVTETKVDE 407

BLAST of Chy12G214910 vs. ExPASy Swiss-Prot
Match: Q0WRY5 (Probable serine/threonine-protein kinase PBL7 OS=Arabidopsis thaliana OX=3702 GN=PBL7 PE=1 SV=1)

HSP 1 Score: 510.8 bits (1314), Expect = 1.5e-143
Identity = 251/358 (70.11%), Postives = 290/358 (81.01%), Query Frame = 0

Query: 46  NLVKRDSDVKVVVSKNDQLALDVKKLNLKDEVSE---EGEANGRAKTFTFEELAAATGNF 105
           N  +R+ D K+    +D      +K   K  +SE   +G  +  A+TFTF ELA AT NF
Sbjct: 14  NKTRRNGDHKLDRKSSDCSVSTSEKSRAKSSLSESKSKGSDHIVAQTFTFSELATATRNF 73

Query: 106 RSDCFLGEGGFGKVYKGYLEKVNEVVAIKQLDRNGLQGIREFVVEVLTLSLADHPNLVKL 165
           R +C +GEGGFG+VYKGYL   ++  AIKQLD NGLQG REF+VEVL LSL  HPNLV L
Sbjct: 74  RKECLIGEGGFGRVYKGYLASTSQTAAIKQLDHNGLQGNREFLVEVLMLSLLHHPNLVNL 133

Query: 166 IGFCAEGDQRLLVYEYMPLGSLENHLHDLRPGAKVIDWNTRMKIAAGAARGLEYLHEKMK 225
           IG+CA+GDQRLLVYEYMPLGSLE+HLHD+ PG + +DWNTRMKIAAGAA+GLEYLH+K  
Sbjct: 134 IGYCADGDQRLLVYEYMPLGSLEDHLHDISPGKQPLDWNTRMKIAAGAAKGLEYLHDKTM 193

Query: 226 PPVIYRDLKCSNILLGEGYHPKLSDFGLAKVGPSGDKTHVSTRVMGTYGYCAPDYAMTGQ 285
           PPVIYRDLKCSNILL + Y PKLSDFGLAK+GP GDK+HVSTRVMGTYGYCAP+YAMTGQ
Sbjct: 194 PPVIYRDLKCSNILLDDDYFPKLSDFGLAKLGPVGDKSHVSTRVMGTYGYCAPEYAMTGQ 253

Query: 286 LTFKSDIYSFGVVLLELITGRKAIDHSKPHSEQNLVAWARPLFRDRKKFSQMVDPMLQGH 345
           LT KSD+YSFGVVLLE+ITGRKAID S+   EQNLVAWARPLF+DR+KFSQM DPMLQG 
Sbjct: 254 LTLKSDVYSFGVVLLEIITGRKAIDSSRSTGEQNLVAWARPLFKDRRKFSQMADPMLQGQ 313

Query: 346 YPVRGLYQSLAIAAMCVQEQPNMRPVITDVVTALNYLASQKYDPQTQPNQNSQKRPSS 401
           YP RGLYQ+LA+AAMCVQEQPN+RP+I DVVTAL+YLASQK+DP  QP Q S   P +
Sbjct: 314 YPPRGLYQALAVAAMCVQEQPNLRPLIADVVTALSYLASQKFDPLAQPVQGSLFAPGT 371

BLAST of Chy12G214910 vs. ExPASy Swiss-Prot
Match: Q1PDV6 (Serine/threonine-protein kinase PBL27 OS=Arabidopsis thaliana OX=3702 GN=PBL27 PE=1 SV=1)

HSP 1 Score: 490.0 bits (1260), Expect = 2.8e-137
Identity = 244/364 (67.03%), Postives = 285/364 (78.30%), Query Frame = 0

Query: 58  VSKNDQLALDVKKLNLKDEVSEE-------GEANGRAKTFTFEELAAATGNFRSDCFLGE 117
           V+++  ++LD  K     E  +E         A+  A+TFTF ELAAAT NFR +C LGE
Sbjct: 32  VTQSHHISLDKSKSRRGPEQKKELTAPKEGPTAHIAAQTFTFRELAAATKNFRPECLLGE 91

Query: 118 GGFGKVYKGYLEKVNEVVAIKQLDRNGLQGIREFVVEVLTLSLADHPNLVKLIGFCAEGD 177
           GGFG+VYKG LE   ++VA+KQLDRNGLQG REF+VEVL LSL  HPNLV LIG+CA+GD
Sbjct: 92  GGFGRVYKGRLETTGQIVAVKQLDRNGLQGNREFLVEVLMLSLLHHPNLVNLIGYCADGD 151

Query: 178 QRLLVYEYMPLGSLENHLHDLRPGAKVIDWNTRMKIAAGAARGLEYLHEKMKPPVIYRDL 237
           QRLLVYEYMPLGSLE+HLHDL P  + +DW+TRM IAAGAA+GLEYLH+K  PPVIYRDL
Sbjct: 152 QRLLVYEYMPLGSLEDHLHDLPPDKEPLDWSTRMTIAAGAAKGLEYLHDKANPPVIYRDL 211

Query: 238 KCSNILLGEGYHPKLSDFGLAKVGPSGDKTHVSTRVMGTYGYCAPDYAMTGQLTFKSDIY 297
           K SNILLG+GYHPKLSDFGLAK+GP GDKTHVSTRVMGTYGYCAP+YAMTGQLT KSD+Y
Sbjct: 212 KSSNILLGDGYHPKLSDFGLAKLGPVGDKTHVSTRVMGTYGYCAPEYAMTGQLTLKSDVY 271

Query: 298 SFGVVLLELITGRKAIDHSKPHSEQNLVAWARPLFRDRKKFSQMVDPMLQGHYPVRGLYQ 357
           SFGVV LELITGRKAID+++   E NLVAWARPLF+DR+KF +M DP LQG YP+RGLYQ
Sbjct: 272 SFGVVFLELITGRKAIDNARAPGEHNLVAWARPLFKDRRKFPKMADPSLQGRYPMRGLYQ 331

Query: 358 SLAIAAMCVQEQPNMRPVITDVVTALNYLASQKYDPQTQPNQNSQKRPSSHKMERSDDKN 415
           +LA+AAMC+QEQ   RP+I DVVTAL YLASQ +DP     QNS+       +   DD+ 
Sbjct: 332 ALAVAAMCLQEQAATRPLIGDVVTALTYLASQTFDPNAPSGQNSRSGSGPPFIRTRDDRR 391

BLAST of Chy12G214910 vs. ExPASy Swiss-Prot
Match: Q6I5Q6 (Receptor-like cytoplasmic kinase 185 OS=Oryza sativa subsp. japonica OX=39947 GN=RLCK185 PE=1 SV=1)

HSP 1 Score: 490.0 bits (1260), Expect = 2.8e-137
Identity = 260/406 (64.04%), Postives = 302/406 (74.38%), Query Frame = 0

Query: 1   MGCFRCTGSSSK---KSEDIEQKDPNTFNSKTHKPDDRTPSDKAKVISNLVKRDSDVKVV 60
           MGCF C GS  K   K     +KD  + + +      R  SDK+K               
Sbjct: 1   MGCFPCFGSGGKGEAKKGGGGRKDGGSADRRV----ARVGSDKSK--------------- 60

Query: 61  VSKNDQLALDVKKLNLKDEVSEEGEANGR---AKTFTFEELAAATGNFRSDCFLGEGGFG 120
                Q  LD +K     +     +ANG+   A TFTF ELAAAT NFR DC LGEGGFG
Sbjct: 61  ----SQGGLDSRK-----DAFIPRDANGQPIAAHTFTFRELAAATKNFRQDCLLGEGGFG 120

Query: 121 KVYKGYLEKVNEVVAIKQLDRNGLQGIREFVVEVLTLSLADHPNLVKLIGFCAEGDQRLL 180
           +VYKG+LE   + VA+KQLDRNGLQG REF+VEVL LSL  H NLV LIG+CA+GDQRLL
Sbjct: 121 RVYKGHLEN-GQAVAVKQLDRNGLQGNREFLVEVLMLSLLHHDNLVNLIGYCADGDQRLL 180

Query: 181 VYEYMPLGSLENHLHDLRPGAKVIDWNTRMKIAAGAARGLEYLHEKMKPPVIYRDLKCSN 240
           VYE+MPLGSLE+HLHD+ P  + +DWNTRMKIAAGAA+GLE+LH+K  PPVIYRD K SN
Sbjct: 181 VYEFMPLGSLEDHLHDIPPDKEPLDWNTRMKIAAGAAKGLEFLHDKANPPVIYRDFKSSN 240

Query: 241 ILLGEGYHPKLSDFGLAKVGPSGDKTHVSTRVMGTYGYCAPDYAMTGQLTFKSDIYSFGV 300
           ILLGEGYHPKLSDFGLAK+GP GDKTHVSTRVMGTYGYCAP+YAMTGQLT KSD+YSFGV
Sbjct: 241 ILLGEGYHPKLSDFGLAKLGPVGDKTHVSTRVMGTYGYCAPEYAMTGQLTVKSDVYSFGV 300

Query: 301 VLLELITGRKAIDHSKPHSEQNLVAWARPLFRDRKKFSQMVDPMLQGHYPVRGLYQSLAI 360
           V LELITGRKAID++KP  EQNLVAWARPLF+DR+KF +M DP+L G +P+RGLYQ+LA+
Sbjct: 301 VFLELITGRKAIDNTKPLGEQNLVAWARPLFKDRRKFPKMADPLLAGRFPMRGLYQALAV 360

Query: 361 AAMCVQEQPNMRPVITDVVTALNYLASQKYDPQTQPNQNSQKRPSS 401
           AAMC+QEQ   RP I DVVTAL+YLASQ YDP T P Q+S+   S+
Sbjct: 361 AAMCLQEQAATRPFIGDVVTALSYLASQTYDPNT-PVQHSRSNAST 376

BLAST of Chy12G214910 vs. ExPASy TrEMBL
Match: A0A0A0LSG4 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G077740 PE=3 SV=1)

HSP 1 Score: 833.6 bits (2152), Expect = 3.8e-238
Identity = 417/421 (99.05%), Postives = 419/421 (99.52%), Query Frame = 0

Query: 1   MGCFRCTGSSSKKSEDIEQKDPNTFNSKTHKPDDRTPSDKAKVISNLVKRDSDVKVVVSK 60
           MGCFRCTGSSSKKSEDIEQKDPNTFNSKTHKPDDRTPSDKAKVISNLVKRDSDVKVVVSK
Sbjct: 1   MGCFRCTGSSSKKSEDIEQKDPNTFNSKTHKPDDRTPSDKAKVISNLVKRDSDVKVVVSK 60

Query: 61  NDQLALDVKKLNLKDEVSEEGEANGRAKTFTFEELAAATGNFRSDCFLGEGGFGKVYKGY 120
           NDQLALDVKKLNLK+EVSEEGEANGRAKTFTFEELAAATGNFRSDCFLGEGGFGKVYKGY
Sbjct: 61  NDQLALDVKKLNLKNEVSEEGEANGRAKTFTFEELAAATGNFRSDCFLGEGGFGKVYKGY 120

Query: 121 LEKVNEVVAIKQLDRNGLQGIREFVVEVLTLSLADHPNLVKLIGFCAEGDQRLLVYEYMP 180
           LEKVNEVVAIKQLDRNGLQGIREFVVEVLTLSLADHPNLVKLIGFCAEGDQRLLVYEYMP
Sbjct: 121 LEKVNEVVAIKQLDRNGLQGIREFVVEVLTLSLADHPNLVKLIGFCAEGDQRLLVYEYMP 180

Query: 181 LGSLENHLHDLRPGAKVIDWNTRMKIAAGAARGLEYLHEKMKPPVIYRDLKCSNILLGEG 240
           LGSLENHLHDLRPGAKVIDWNTRMKIAAGAARGLEYLHEKMKPPVIYRDLKCSNILLGEG
Sbjct: 181 LGSLENHLHDLRPGAKVIDWNTRMKIAAGAARGLEYLHEKMKPPVIYRDLKCSNILLGEG 240

Query: 241 YHPKLSDFGLAKVGPSGDKTHVSTRVMGTYGYCAPDYAMTGQLTFKSDIYSFGVVLLELI 300
           YHPKLSDFGLAKVGPSGDKTHVSTRVMGTYGYCAPDYAMTGQLTFKSDIYSFGVVLLELI
Sbjct: 241 YHPKLSDFGLAKVGPSGDKTHVSTRVMGTYGYCAPDYAMTGQLTFKSDIYSFGVVLLELI 300

Query: 301 TGRKAIDHSKPHSEQNLVAWARPLFRDRKKFSQMVDPMLQGHYPVRGLYQSLAIAAMCVQ 360
           TGRKAIDHSKPHSEQNLVAWARP+FRDRKKFSQMVDPMLQGHYPVRGLYQSLAIAAMCVQ
Sbjct: 301 TGRKAIDHSKPHSEQNLVAWARPMFRDRKKFSQMVDPMLQGHYPVRGLYQSLAIAAMCVQ 360

Query: 361 EQPNMRPVITDVVTALNYLASQKYDPQTQPNQNSQKRPSSHKMERSDDKNETSDNDESER 420
           EQPNMRPVITDVVTALNYLASQKYDPQTQPNQNSQKRPSSHKMER DDK ETSDNDESER
Sbjct: 361 EQPNMRPVITDVVTALNYLASQKYDPQTQPNQNSQKRPSSHKMERGDDKKETSDNDESER 420

Query: 421 E 422
           E
Sbjct: 421 E 421

BLAST of Chy12G214910 vs. ExPASy TrEMBL
Match: A0A5D3DCD2 (Putative serine/threonine-protein kinase RLCKVII OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold359G00410 PE=3 SV=1)

HSP 1 Score: 826.6 bits (2134), Expect = 4.6e-236
Identity = 414/421 (98.34%), Postives = 417/421 (99.05%), Query Frame = 0

Query: 1   MGCFRCTGSSSKKSEDIEQKDPNTFNSKTHKPDDRTPSDKAKVISNLVKRDSDVKVVVSK 60
           MGCFRCTGSSSKKSE+IEQ +PNTFNSKTHKPDDRTPSDKAKVISNLVKRDSDVKVVVSK
Sbjct: 1   MGCFRCTGSSSKKSENIEQNNPNTFNSKTHKPDDRTPSDKAKVISNLVKRDSDVKVVVSK 60

Query: 61  NDQLALDVKKLNLKDEVSEEGEANGRAKTFTFEELAAATGNFRSDCFLGEGGFGKVYKGY 120
           NDQLALDVKKLNLKDEVSEEGEANGRAKTFTFEELAAATGNFRSDCFLGEGGFGKVYKGY
Sbjct: 61  NDQLALDVKKLNLKDEVSEEGEANGRAKTFTFEELAAATGNFRSDCFLGEGGFGKVYKGY 120

Query: 121 LEKVNEVVAIKQLDRNGLQGIREFVVEVLTLSLADHPNLVKLIGFCAEGDQRLLVYEYMP 180
           L+KVNEVVAIKQLDRNGLQGIREFVVEVLTLSLADHPNLVKLIGFCAEGDQRLLVYEYMP
Sbjct: 121 LQKVNEVVAIKQLDRNGLQGIREFVVEVLTLSLADHPNLVKLIGFCAEGDQRLLVYEYMP 180

Query: 181 LGSLENHLHDLRPGAKVIDWNTRMKIAAGAARGLEYLHEKMKPPVIYRDLKCSNILLGEG 240
           LGSLENHLHDLRPGAKVIDWNTRMKIAAGAARGLEYLHEKMKPPVIYRDLKCSNILLGEG
Sbjct: 181 LGSLENHLHDLRPGAKVIDWNTRMKIAAGAARGLEYLHEKMKPPVIYRDLKCSNILLGEG 240

Query: 241 YHPKLSDFGLAKVGPSGDKTHVSTRVMGTYGYCAPDYAMTGQLTFKSDIYSFGVVLLELI 300
           YHPKLSDFGLAKVGPSGDKTHVSTRVMGTYGYCAPDYAMTGQLTFKSDIYSFGVVLLELI
Sbjct: 241 YHPKLSDFGLAKVGPSGDKTHVSTRVMGTYGYCAPDYAMTGQLTFKSDIYSFGVVLLELI 300

Query: 301 TGRKAIDHSKPHSEQNLVAWARPLFRDRKKFSQMVDPMLQGHYPVRGLYQSLAIAAMCVQ 360
           TGRKAIDHSKPHSEQNLVAWARPLFRDRKKFSQMVDPMLQGHYPVRGLYQSLAIAAMCVQ
Sbjct: 301 TGRKAIDHSKPHSEQNLVAWARPLFRDRKKFSQMVDPMLQGHYPVRGLYQSLAIAAMCVQ 360

Query: 361 EQPNMRPVITDVVTALNYLASQKYDPQTQPNQNSQKRPSSHKMERSDDKNETSDNDESER 420
           EQPNMRPVITDVVTALNYLASQKYDPQTQPNQNSQKRPSSHKMER DDK ETSDND SER
Sbjct: 361 EQPNMRPVITDVVTALNYLASQKYDPQTQPNQNSQKRPSSHKMERGDDKRETSDNDGSER 420

Query: 421 E 422
           E
Sbjct: 421 E 421

BLAST of Chy12G214910 vs. ExPASy TrEMBL
Match: A0A1S3CNI7 (probable serine/threonine-protein kinase RLCKVII OS=Cucumis melo OX=3656 GN=LOC103502940 PE=3 SV=1)

HSP 1 Score: 826.6 bits (2134), Expect = 4.6e-236
Identity = 414/421 (98.34%), Postives = 417/421 (99.05%), Query Frame = 0

Query: 1   MGCFRCTGSSSKKSEDIEQKDPNTFNSKTHKPDDRTPSDKAKVISNLVKRDSDVKVVVSK 60
           MGCFRCTGSSSKKSE+IEQ +PNTFNSKTHKPDDRTPSDKAKVISNLVKRDSDVKVVVSK
Sbjct: 1   MGCFRCTGSSSKKSENIEQNNPNTFNSKTHKPDDRTPSDKAKVISNLVKRDSDVKVVVSK 60

Query: 61  NDQLALDVKKLNLKDEVSEEGEANGRAKTFTFEELAAATGNFRSDCFLGEGGFGKVYKGY 120
           NDQLALDVKKLNLKDEVSEEGEANGRAKTFTFEELAAATGNFRSDCFLGEGGFGKVYKGY
Sbjct: 61  NDQLALDVKKLNLKDEVSEEGEANGRAKTFTFEELAAATGNFRSDCFLGEGGFGKVYKGY 120

Query: 121 LEKVNEVVAIKQLDRNGLQGIREFVVEVLTLSLADHPNLVKLIGFCAEGDQRLLVYEYMP 180
           L+KVNEVVAIKQLDRNGLQGIREFVVEVLTLSLADHPNLVKLIGFCAEGDQRLLVYEYMP
Sbjct: 121 LQKVNEVVAIKQLDRNGLQGIREFVVEVLTLSLADHPNLVKLIGFCAEGDQRLLVYEYMP 180

Query: 181 LGSLENHLHDLRPGAKVIDWNTRMKIAAGAARGLEYLHEKMKPPVIYRDLKCSNILLGEG 240
           LGSLENHLHDLRPGAKVIDWNTRMKIAAGAARGLEYLHEKMKPPVIYRDLKCSNILLGEG
Sbjct: 181 LGSLENHLHDLRPGAKVIDWNTRMKIAAGAARGLEYLHEKMKPPVIYRDLKCSNILLGEG 240

Query: 241 YHPKLSDFGLAKVGPSGDKTHVSTRVMGTYGYCAPDYAMTGQLTFKSDIYSFGVVLLELI 300
           YHPKLSDFGLAKVGPSGDKTHVSTRVMGTYGYCAPDYAMTGQLTFKSDIYSFGVVLLELI
Sbjct: 241 YHPKLSDFGLAKVGPSGDKTHVSTRVMGTYGYCAPDYAMTGQLTFKSDIYSFGVVLLELI 300

Query: 301 TGRKAIDHSKPHSEQNLVAWARPLFRDRKKFSQMVDPMLQGHYPVRGLYQSLAIAAMCVQ 360
           TGRKAIDHSKPHSEQNLVAWARPLFRDRKKFSQMVDPMLQGHYPVRGLYQSLAIAAMCVQ
Sbjct: 301 TGRKAIDHSKPHSEQNLVAWARPLFRDRKKFSQMVDPMLQGHYPVRGLYQSLAIAAMCVQ 360

Query: 361 EQPNMRPVITDVVTALNYLASQKYDPQTQPNQNSQKRPSSHKMERSDDKNETSDNDESER 420
           EQPNMRPVITDVVTALNYLASQKYDPQTQPNQNSQKRPSSHKMER DDK ETSDND SER
Sbjct: 361 EQPNMRPVITDVVTALNYLASQKYDPQTQPNQNSQKRPSSHKMERGDDKRETSDNDGSER 420

Query: 421 E 422
           E
Sbjct: 421 E 421

BLAST of Chy12G214910 vs. ExPASy TrEMBL
Match: A0A6J1I3P0 (probable serine/threonine-protein kinase PBL5 OS=Cucurbita maxima OX=3661 GN=LOC111469375 PE=3 SV=1)

HSP 1 Score: 781.9 bits (2018), Expect = 1.3e-222
Identity = 394/422 (93.36%), Postives = 405/422 (95.97%), Query Frame = 0

Query: 1   MGCFRCTGSSSKKSEDIEQKDPNTFNSKTHKPDDRTPSDKAKVISNL-VKRDSDVKVVVS 60
           MGCF C GSSSKK E IEQ +PNTFNSKTHKPDDRTPSDK KV S+L VKRDSDVKVVVS
Sbjct: 1   MGCFCCFGSSSKKPEKIEQNNPNTFNSKTHKPDDRTPSDKPKVNSSLAVKRDSDVKVVVS 60

Query: 61  KNDQLALDVKKLNLKDEVSEEGEANGRAKTFTFEELAAATGNFRSDCFLGEGGFGKVYKG 120
           K+DQLALDVKKLNLKDEVSEEGE NGRAKTFTFEEL AATGNFRSDCFLGEGGFGKVYKG
Sbjct: 61  KDDQLALDVKKLNLKDEVSEEGETNGRAKTFTFEELKAATGNFRSDCFLGEGGFGKVYKG 120

Query: 121 YLEKVNEVVAIKQLDRNGLQGIREFVVEVLTLSLADHPNLVKLIGFCAEGDQRLLVYEYM 180
           YL++V+EVVAIKQLDRNG+QGIREFVVEVLTLSLADHPNLVKLIGFCAEGDQRLLVYEYM
Sbjct: 121 YLQQVSEVVAIKQLDRNGVQGIREFVVEVLTLSLADHPNLVKLIGFCAEGDQRLLVYEYM 180

Query: 181 PLGSLENHLHDLRPGAKVIDWNTRMKIAAGAARGLEYLHEKMKPPVIYRDLKCSNILLGE 240
           PLGSLENHLHDLRPG KVIDWNTRMKIAAGAA+GLEYLHEKMKPPVIYRDLKCSNILLGE
Sbjct: 181 PLGSLENHLHDLRPGVKVIDWNTRMKIAAGAAKGLEYLHEKMKPPVIYRDLKCSNILLGE 240

Query: 241 GYHPKLSDFGLAKVGPSGDKTHVSTRVMGTYGYCAPDYAMTGQLTFKSDIYSFGVVLLEL 300
           GYHPKLSDFGLAKVGPSGDKTHVSTRVMGTYGYCAPDYAMTGQLTFKSDIYSFGVVLLEL
Sbjct: 241 GYHPKLSDFGLAKVGPSGDKTHVSTRVMGTYGYCAPDYAMTGQLTFKSDIYSFGVVLLEL 300

Query: 301 ITGRKAIDHSKPHSEQNLVAWARPLFRDRKKFSQMVDPMLQGHYPVRGLYQSLAIAAMCV 360
           ITGRKAIDHSKPHSEQNLVAWARPLFRDRKKFSQMVDPMLQGHYPVRGLYQSLAIAAMCV
Sbjct: 301 ITGRKAIDHSKPHSEQNLVAWARPLFRDRKKFSQMVDPMLQGHYPVRGLYQSLAIAAMCV 360

Query: 361 QEQPNMRPVITDVVTALNYLASQKYDPQTQPNQNSQKRPSSHKMERSDDKNETSDNDESE 420
           QEQPNMRPVI+DVVTALNYLASQKYDPQTQPN +SQKRP S KMER DDK  TSD+DESE
Sbjct: 361 QEQPNMRPVISDVVTALNYLASQKYDPQTQPNHSSQKRPPSPKMERDDDKRHTSDDDESE 420

Query: 421 RE 422
           RE
Sbjct: 421 RE 422

BLAST of Chy12G214910 vs. ExPASy TrEMBL
Match: A0A6J1HJX5 (probable serine/threonine-protein kinase PBL5 OS=Cucurbita moschata OX=3662 GN=LOC111464790 PE=3 SV=1)

HSP 1 Score: 776.9 bits (2005), Expect = 4.2e-221
Identity = 391/422 (92.65%), Postives = 403/422 (95.50%), Query Frame = 0

Query: 1   MGCFRCTGSSSKKSEDIEQKDPNTFNSKTHKPDDRTPSDKAKVISNL-VKRDSDVKVVVS 60
           MGCF C GSSSKK E IEQ +PN FNSKTHKPDDRTPSDK KV S+L VKRDSDVKVVVS
Sbjct: 1   MGCFCCFGSSSKKPEKIEQNNPNAFNSKTHKPDDRTPSDKPKVNSSLAVKRDSDVKVVVS 60

Query: 61  KNDQLALDVKKLNLKDEVSEEGEANGRAKTFTFEELAAATGNFRSDCFLGEGGFGKVYKG 120
           K+DQLALDVKKLNLKDEVSE+GE NGRAKTFTFEEL AATGNFRSDCFLGEGGFGKVYKG
Sbjct: 61  KDDQLALDVKKLNLKDEVSEDGETNGRAKTFTFEELKAATGNFRSDCFLGEGGFGKVYKG 120

Query: 121 YLEKVNEVVAIKQLDRNGLQGIREFVVEVLTLSLADHPNLVKLIGFCAEGDQRLLVYEYM 180
           YL++V+E+VAIKQLDRNG+QGIREFVVEVLTLSLADHPNLVKLIGFCAEGDQRLLVYEYM
Sbjct: 121 YLQQVSEIVAIKQLDRNGVQGIREFVVEVLTLSLADHPNLVKLIGFCAEGDQRLLVYEYM 180

Query: 181 PLGSLENHLHDLRPGAKVIDWNTRMKIAAGAARGLEYLHEKMKPPVIYRDLKCSNILLGE 240
           PLGSLENHLHDLRPG KVIDWNTRMKIAAGAA+GLEYLHEKMKPPVIYRDLKCSNILLGE
Sbjct: 181 PLGSLENHLHDLRPGVKVIDWNTRMKIAAGAAKGLEYLHEKMKPPVIYRDLKCSNILLGE 240

Query: 241 GYHPKLSDFGLAKVGPSGDKTHVSTRVMGTYGYCAPDYAMTGQLTFKSDIYSFGVVLLEL 300
           GYHPKLSDFGLAKVGPSGDKTHVSTRVMGTYGYCAPDYAMTGQLTFKSDIYSFGVVLLEL
Sbjct: 241 GYHPKLSDFGLAKVGPSGDKTHVSTRVMGTYGYCAPDYAMTGQLTFKSDIYSFGVVLLEL 300

Query: 301 ITGRKAIDHSKPHSEQNLVAWARPLFRDRKKFSQMVDPMLQGHYPVRGLYQSLAIAAMCV 360
           ITGRKAIDHSKPHSEQNLVAWARPLFRDRKKFSQMVDPMLQGHYPVRGLYQSLAIAAMCV
Sbjct: 301 ITGRKAIDHSKPHSEQNLVAWARPLFRDRKKFSQMVDPMLQGHYPVRGLYQSLAIAAMCV 360

Query: 361 QEQPNMRPVITDVVTALNYLASQKYDPQTQPNQNSQKRPSSHKMERSDDKNETSDNDESE 420
           QEQPNMRPVI+DVVTALNYLASQKYDPQTQPN  SQKRP S KMER DDK  TSD+DESE
Sbjct: 361 QEQPNMRPVISDVVTALNYLASQKYDPQTQPNHISQKRPPSPKMERDDDKRHTSDDDESE 420

Query: 421 RE 422
           RE
Sbjct: 421 RE 422

BLAST of Chy12G214910 vs. NCBI nr
Match: XP_004148777.1 (probable serine/threonine-protein kinase PBL5 [Cucumis sativus] >KGN64723.1 hypothetical protein Csa_013577 [Cucumis sativus])

HSP 1 Score: 826 bits (2134), Expect = 9.45e-302
Identity = 417/421 (99.05%), Postives = 419/421 (99.52%), Query Frame = 0

Query: 1   MGCFRCTGSSSKKSEDIEQKDPNTFNSKTHKPDDRTPSDKAKVISNLVKRDSDVKVVVSK 60
           MGCFRCTGSSSKKSEDIEQKDPNTFNSKTHKPDDRTPSDKAKVISNLVKRDSDVKVVVSK
Sbjct: 1   MGCFRCTGSSSKKSEDIEQKDPNTFNSKTHKPDDRTPSDKAKVISNLVKRDSDVKVVVSK 60

Query: 61  NDQLALDVKKLNLKDEVSEEGEANGRAKTFTFEELAAATGNFRSDCFLGEGGFGKVYKGY 120
           NDQLALDVKKLNLK+EVSEEGEANGRAKTFTFEELAAATGNFRSDCFLGEGGFGKVYKGY
Sbjct: 61  NDQLALDVKKLNLKNEVSEEGEANGRAKTFTFEELAAATGNFRSDCFLGEGGFGKVYKGY 120

Query: 121 LEKVNEVVAIKQLDRNGLQGIREFVVEVLTLSLADHPNLVKLIGFCAEGDQRLLVYEYMP 180
           LEKVNEVVAIKQLDRNGLQGIREFVVEVLTLSLADHPNLVKLIGFCAEGDQRLLVYEYMP
Sbjct: 121 LEKVNEVVAIKQLDRNGLQGIREFVVEVLTLSLADHPNLVKLIGFCAEGDQRLLVYEYMP 180

Query: 181 LGSLENHLHDLRPGAKVIDWNTRMKIAAGAARGLEYLHEKMKPPVIYRDLKCSNILLGEG 240
           LGSLENHLHDLRPGAKVIDWNTRMKIAAGAARGLEYLHEKMKPPVIYRDLKCSNILLGEG
Sbjct: 181 LGSLENHLHDLRPGAKVIDWNTRMKIAAGAARGLEYLHEKMKPPVIYRDLKCSNILLGEG 240

Query: 241 YHPKLSDFGLAKVGPSGDKTHVSTRVMGTYGYCAPDYAMTGQLTFKSDIYSFGVVLLELI 300
           YHPKLSDFGLAKVGPSGDKTHVSTRVMGTYGYCAPDYAMTGQLTFKSDIYSFGVVLLELI
Sbjct: 241 YHPKLSDFGLAKVGPSGDKTHVSTRVMGTYGYCAPDYAMTGQLTFKSDIYSFGVVLLELI 300

Query: 301 TGRKAIDHSKPHSEQNLVAWARPLFRDRKKFSQMVDPMLQGHYPVRGLYQSLAIAAMCVQ 360
           TGRKAIDHSKPHSEQNLVAWARP+FRDRKKFSQMVDPMLQGHYPVRGLYQSLAIAAMCVQ
Sbjct: 301 TGRKAIDHSKPHSEQNLVAWARPMFRDRKKFSQMVDPMLQGHYPVRGLYQSLAIAAMCVQ 360

Query: 361 EQPNMRPVITDVVTALNYLASQKYDPQTQPNQNSQKRPSSHKMERSDDKNETSDNDESER 420
           EQPNMRPVITDVVTALNYLASQKYDPQTQPNQNSQKRPSSHKMER DDK ETSDNDESER
Sbjct: 361 EQPNMRPVITDVVTALNYLASQKYDPQTQPNQNSQKRPSSHKMERGDDKKETSDNDESER 420

BLAST of Chy12G214910 vs. NCBI nr
Match: XP_008465289.1 (PREDICTED: probable serine/threonine-protein kinase RLCKVII [Cucumis melo] >TYK21232.1 putative serine/threonine-protein kinase RLCKVII [Cucumis melo var. makuwa])

HSP 1 Score: 819 bits (2116), Expect = 5.24e-299
Identity = 414/421 (98.34%), Postives = 417/421 (99.05%), Query Frame = 0

Query: 1   MGCFRCTGSSSKKSEDIEQKDPNTFNSKTHKPDDRTPSDKAKVISNLVKRDSDVKVVVSK 60
           MGCFRCTGSSSKKSE+IEQ +PNTFNSKTHKPDDRTPSDKAKVISNLVKRDSDVKVVVSK
Sbjct: 1   MGCFRCTGSSSKKSENIEQNNPNTFNSKTHKPDDRTPSDKAKVISNLVKRDSDVKVVVSK 60

Query: 61  NDQLALDVKKLNLKDEVSEEGEANGRAKTFTFEELAAATGNFRSDCFLGEGGFGKVYKGY 120
           NDQLALDVKKLNLKDEVSEEGEANGRAKTFTFEELAAATGNFRSDCFLGEGGFGKVYKGY
Sbjct: 61  NDQLALDVKKLNLKDEVSEEGEANGRAKTFTFEELAAATGNFRSDCFLGEGGFGKVYKGY 120

Query: 121 LEKVNEVVAIKQLDRNGLQGIREFVVEVLTLSLADHPNLVKLIGFCAEGDQRLLVYEYMP 180
           L+KVNEVVAIKQLDRNGLQGIREFVVEVLTLSLADHPNLVKLIGFCAEGDQRLLVYEYMP
Sbjct: 121 LQKVNEVVAIKQLDRNGLQGIREFVVEVLTLSLADHPNLVKLIGFCAEGDQRLLVYEYMP 180

Query: 181 LGSLENHLHDLRPGAKVIDWNTRMKIAAGAARGLEYLHEKMKPPVIYRDLKCSNILLGEG 240
           LGSLENHLHDLRPGAKVIDWNTRMKIAAGAARGLEYLHEKMKPPVIYRDLKCSNILLGEG
Sbjct: 181 LGSLENHLHDLRPGAKVIDWNTRMKIAAGAARGLEYLHEKMKPPVIYRDLKCSNILLGEG 240

Query: 241 YHPKLSDFGLAKVGPSGDKTHVSTRVMGTYGYCAPDYAMTGQLTFKSDIYSFGVVLLELI 300
           YHPKLSDFGLAKVGPSGDKTHVSTRVMGTYGYCAPDYAMTGQLTFKSDIYSFGVVLLELI
Sbjct: 241 YHPKLSDFGLAKVGPSGDKTHVSTRVMGTYGYCAPDYAMTGQLTFKSDIYSFGVVLLELI 300

Query: 301 TGRKAIDHSKPHSEQNLVAWARPLFRDRKKFSQMVDPMLQGHYPVRGLYQSLAIAAMCVQ 360
           TGRKAIDHSKPHSEQNLVAWARPLFRDRKKFSQMVDPMLQGHYPVRGLYQSLAIAAMCVQ
Sbjct: 301 TGRKAIDHSKPHSEQNLVAWARPLFRDRKKFSQMVDPMLQGHYPVRGLYQSLAIAAMCVQ 360

Query: 361 EQPNMRPVITDVVTALNYLASQKYDPQTQPNQNSQKRPSSHKMERSDDKNETSDNDESER 420
           EQPNMRPVITDVVTALNYLASQKYDPQTQPNQNSQKRPSSHKMER DDK ETSDND SER
Sbjct: 361 EQPNMRPVITDVVTALNYLASQKYDPQTQPNQNSQKRPSSHKMERGDDKRETSDNDGSER 420

BLAST of Chy12G214910 vs. NCBI nr
Match: XP_038895614.1 (probable serine/threonine-protein kinase PBL5 [Benincasa hispida])

HSP 1 Score: 787 bits (2033), Expect = 2.43e-286
Identity = 400/422 (94.79%), Postives = 412/422 (97.63%), Query Frame = 0

Query: 1   MGCFRCTGSSSKKSEDIEQKDPNTFNSKTHKPDDRTPSDKAKVISNL-VKRDSDVKVVVS 60
           MGCFRCTGSSSKKSE+IE+ +PNTFNSKTHKPDDR PSDKAK+ S+L VKRDSDVKVVVS
Sbjct: 1   MGCFRCTGSSSKKSENIEENNPNTFNSKTHKPDDRMPSDKAKINSSLAVKRDSDVKVVVS 60

Query: 61  KNDQLALDVKKLNLKDEVSEEGEANGRAKTFTFEELAAATGNFRSDCFLGEGGFGKVYKG 120
           K+DQLALDVK+LNLKDEVSEEGE+NGRAKTFTFEELAAATGNFRSDCFLGEGGFGKVYKG
Sbjct: 61  KDDQLALDVKELNLKDEVSEEGESNGRAKTFTFEELAAATGNFRSDCFLGEGGFGKVYKG 120

Query: 121 YLEKVNEVVAIKQLDRNGLQGIREFVVEVLTLSLADHPNLVKLIGFCAEGDQRLLVYEYM 180
           YL+KVNEVVAIKQLDRNGLQGIREFVVEVLTLSLADHPNLVKLIGFCAEGDQRLLVYEYM
Sbjct: 121 YLQKVNEVVAIKQLDRNGLQGIREFVVEVLTLSLADHPNLVKLIGFCAEGDQRLLVYEYM 180

Query: 181 PLGSLENHLHDLRPGAKVIDWNTRMKIAAGAARGLEYLHEKMKPPVIYRDLKCSNILLGE 240
           PLGSLENHLHDLRPGAKVIDWNTRMKIAAGAARGLEYLHEKMKPPVIYRDLKCSNILLGE
Sbjct: 181 PLGSLENHLHDLRPGAKVIDWNTRMKIAAGAARGLEYLHEKMKPPVIYRDLKCSNILLGE 240

Query: 241 GYHPKLSDFGLAKVGPSGDKTHVSTRVMGTYGYCAPDYAMTGQLTFKSDIYSFGVVLLEL 300
           GYHPKLSDFGLAKVGPSGDKTHVSTRVMGTYGYCAPDYAMTGQLTFKSDIYSFGVVLLEL
Sbjct: 241 GYHPKLSDFGLAKVGPSGDKTHVSTRVMGTYGYCAPDYAMTGQLTFKSDIYSFGVVLLEL 300

Query: 301 ITGRKAIDHSKPHSEQNLVAWARPLFRDRKKFSQMVDPMLQGHYPVRGLYQSLAIAAMCV 360
           ITGRKAIDHSKPHSEQNLVAWARPLFRDRKKFSQMVDPMLQG YPVRGLYQSLAIAAMCV
Sbjct: 301 ITGRKAIDHSKPHSEQNLVAWARPLFRDRKKFSQMVDPMLQGQYPVRGLYQSLAIAAMCV 360

Query: 361 QEQPNMRPVITDVVTALNYLASQKYDPQTQPNQNSQKRPSSHKMERSDDKNETSDNDESE 420
           QEQPNMRPVI+DVVTALNYLASQKYDPQTQPNQ+SQKRPSS KMER +D  ETSDND SE
Sbjct: 361 QEQPNMRPVISDVVTALNYLASQKYDPQTQPNQSSQKRPSSLKMERDNDTRETSDNDRSE 420

BLAST of Chy12G214910 vs. NCBI nr
Match: XP_022970373.1 (probable serine/threonine-protein kinase PBL5 [Cucurbita maxima])

HSP 1 Score: 775 bits (2000), Expect = 2.59e-281
Identity = 394/422 (93.36%), Postives = 405/422 (95.97%), Query Frame = 0

Query: 1   MGCFRCTGSSSKKSEDIEQKDPNTFNSKTHKPDDRTPSDKAKVISNL-VKRDSDVKVVVS 60
           MGCF C GSSSKK E IEQ +PNTFNSKTHKPDDRTPSDK KV S+L VKRDSDVKVVVS
Sbjct: 1   MGCFCCFGSSSKKPEKIEQNNPNTFNSKTHKPDDRTPSDKPKVNSSLAVKRDSDVKVVVS 60

Query: 61  KNDQLALDVKKLNLKDEVSEEGEANGRAKTFTFEELAAATGNFRSDCFLGEGGFGKVYKG 120
           K+DQLALDVKKLNLKDEVSEEGE NGRAKTFTFEEL AATGNFRSDCFLGEGGFGKVYKG
Sbjct: 61  KDDQLALDVKKLNLKDEVSEEGETNGRAKTFTFEELKAATGNFRSDCFLGEGGFGKVYKG 120

Query: 121 YLEKVNEVVAIKQLDRNGLQGIREFVVEVLTLSLADHPNLVKLIGFCAEGDQRLLVYEYM 180
           YL++V+EVVAIKQLDRNG+QGIREFVVEVLTLSLADHPNLVKLIGFCAEGDQRLLVYEYM
Sbjct: 121 YLQQVSEVVAIKQLDRNGVQGIREFVVEVLTLSLADHPNLVKLIGFCAEGDQRLLVYEYM 180

Query: 181 PLGSLENHLHDLRPGAKVIDWNTRMKIAAGAARGLEYLHEKMKPPVIYRDLKCSNILLGE 240
           PLGSLENHLHDLRPG KVIDWNTRMKIAAGAA+GLEYLHEKMKPPVIYRDLKCSNILLGE
Sbjct: 181 PLGSLENHLHDLRPGVKVIDWNTRMKIAAGAAKGLEYLHEKMKPPVIYRDLKCSNILLGE 240

Query: 241 GYHPKLSDFGLAKVGPSGDKTHVSTRVMGTYGYCAPDYAMTGQLTFKSDIYSFGVVLLEL 300
           GYHPKLSDFGLAKVGPSGDKTHVSTRVMGTYGYCAPDYAMTGQLTFKSDIYSFGVVLLEL
Sbjct: 241 GYHPKLSDFGLAKVGPSGDKTHVSTRVMGTYGYCAPDYAMTGQLTFKSDIYSFGVVLLEL 300

Query: 301 ITGRKAIDHSKPHSEQNLVAWARPLFRDRKKFSQMVDPMLQGHYPVRGLYQSLAIAAMCV 360
           ITGRKAIDHSKPHSEQNLVAWARPLFRDRKKFSQMVDPMLQGHYPVRGLYQSLAIAAMCV
Sbjct: 301 ITGRKAIDHSKPHSEQNLVAWARPLFRDRKKFSQMVDPMLQGHYPVRGLYQSLAIAAMCV 360

Query: 361 QEQPNMRPVITDVVTALNYLASQKYDPQTQPNQNSQKRPSSHKMERSDDKNETSDNDESE 420
           QEQPNMRPVI+DVVTALNYLASQKYDPQTQPN +SQKRP S KMER DDK  TSD+DESE
Sbjct: 361 QEQPNMRPVISDVVTALNYLASQKYDPQTQPNHSSQKRPPSPKMERDDDKRHTSDDDESE 420

BLAST of Chy12G214910 vs. NCBI nr
Match: XP_023519827.1 (probable serine/threonine-protein kinase PBL5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 771 bits (1991), Expect = 6.09e-280
Identity = 392/422 (92.89%), Postives = 404/422 (95.73%), Query Frame = 0

Query: 1   MGCFRCTGSSSKKSEDIEQKDPNTFNSKTHKPDDRTPSDKAKVISNL-VKRDSDVKVVVS 60
           MGCF C GSSSKK E IEQ +PNTFNSKTHKPDDRTPSDK KV S+L VKRDSDVKVVVS
Sbjct: 1   MGCFCCFGSSSKKPEKIEQNNPNTFNSKTHKPDDRTPSDKPKVNSSLAVKRDSDVKVVVS 60

Query: 61  KNDQLALDVKKLNLKDEVSEEGEANGRAKTFTFEELAAATGNFRSDCFLGEGGFGKVYKG 120
           K+DQLALDVKKLNLKDEVSE+GE NGRAKTFTFEEL AATGNFRSDCFLGEGGFGKVYKG
Sbjct: 61  KDDQLALDVKKLNLKDEVSEDGETNGRAKTFTFEELKAATGNFRSDCFLGEGGFGKVYKG 120

Query: 121 YLEKVNEVVAIKQLDRNGLQGIREFVVEVLTLSLADHPNLVKLIGFCAEGDQRLLVYEYM 180
           YL++V+EVVAIKQLDRNG+QGIREFVVEVLTLSLADHPNLVKLIGFCAEGDQRLLVYEYM
Sbjct: 121 YLQQVSEVVAIKQLDRNGVQGIREFVVEVLTLSLADHPNLVKLIGFCAEGDQRLLVYEYM 180

Query: 181 PLGSLENHLHDLRPGAKVIDWNTRMKIAAGAARGLEYLHEKMKPPVIYRDLKCSNILLGE 240
           PLGSLENHLHDLRP  KVIDWNTRMKIAAGAA+GLEYLHEKMKPPVIYRDLKCSNILLGE
Sbjct: 181 PLGSLENHLHDLRPSVKVIDWNTRMKIAAGAAKGLEYLHEKMKPPVIYRDLKCSNILLGE 240

Query: 241 GYHPKLSDFGLAKVGPSGDKTHVSTRVMGTYGYCAPDYAMTGQLTFKSDIYSFGVVLLEL 300
           GYHPKLSDFGLAKVGPSGDKTHVSTRVMGTYGYCAPDYAMTGQLTFKSDIYSFGVVLLEL
Sbjct: 241 GYHPKLSDFGLAKVGPSGDKTHVSTRVMGTYGYCAPDYAMTGQLTFKSDIYSFGVVLLEL 300

Query: 301 ITGRKAIDHSKPHSEQNLVAWARPLFRDRKKFSQMVDPMLQGHYPVRGLYQSLAIAAMCV 360
           ITGRKAIDHSKPHSEQNLVAWARPLFRDRKKFSQMVDPMLQGHYPVRGLYQSLAIAAMCV
Sbjct: 301 ITGRKAIDHSKPHSEQNLVAWARPLFRDRKKFSQMVDPMLQGHYPVRGLYQSLAIAAMCV 360

Query: 361 QEQPNMRPVITDVVTALNYLASQKYDPQTQPNQNSQKRPSSHKMERSDDKNETSDNDESE 420
           QEQPNMRPVI+DVVTALNYLASQKYDPQTQPN +SQKRP S KMER DDK  TSD+DESE
Sbjct: 361 QEQPNMRPVISDVVTALNYLASQKYDPQTQPNHSSQKRPPSPKMERDDDKRHTSDDDESE 420

BLAST of Chy12G214910 vs. TAIR 10
Match: AT1G07870.1 (Protein kinase superfamily protein )

HSP 1 Score: 568.5 bits (1464), Expect = 4.3e-162
Identity = 302/429 (70.40%), Postives = 349/429 (81.35%), Query Frame = 0

Query: 1   MGCFRCTGSSSKKSEDIEQKDPN-------TFNSKTHKPDDRT-PSDKAKVISNLVKRDS 60
           MGCF C+  SSK+SE  +    N       T  +K+ K DD+T PS  +  +S    RD 
Sbjct: 1   MGCFGCSKKSSKRSETNKDTVINRKIVGGTTSVAKSDKRDDQTQPSSDSTKVSPY--RDV 60

Query: 61  DVKVVVSKNDQLALDVKKLNLKDEVSEEGEANGRAKTFTFEELAAATGNFRSDCFLGEGG 120
           + +  V K DQL+LDVK LNL D+V+ +     +A+TFTF+ELA ATGNFRSDCFLGEGG
Sbjct: 61  NNEGGVGKEDQLSLDVKGLNLNDQVTGK-----KAQTFTFQELAEATGNFRSDCFLGEGG 120

Query: 121 FGKVYKGYLEKVNEVVAIKQLDRNGLQGIREFVVEVLTLSLADHPNLVKLIGFCAEGDQR 180
           FGKV+KG +EK+++VVAIKQLDRNG+QGIREFVVEVLTLSLADHPNLVKLIGFCAEGDQR
Sbjct: 121 FGKVFKGTIEKLDQVVAIKQLDRNGVQGIREFVVEVLTLSLADHPNLVKLIGFCAEGDQR 180

Query: 181 LLVYEYMPLGSLENHLHDLRPGAKVIDWNTRMKIAAGAARGLEYLHEKMKPPVIYRDLKC 240
           LLVYEYMP GSLE+HLH L  G K +DWNTRMKIAAGAARGLEYLH++M PPVIYRDLKC
Sbjct: 181 LLVYEYMPQGSLEDHLHVLPSGKKPLDWNTRMKIAAGAARGLEYLHDRMTPPVIYRDLKC 240

Query: 241 SNILLGEGYHPKLSDFGLAKVGPSGDKTHVSTRVMGTYGYCAPDYAMTGQLTFKSDIYSF 300
           SNILLGE Y PKLSDFGLAKVGPSGDKTHVSTRVMGTYGYCAPDYAMTGQLTFKSDIYSF
Sbjct: 241 SNILLGEDYQPKLSDFGLAKVGPSGDKTHVSTRVMGTYGYCAPDYAMTGQLTFKSDIYSF 300

Query: 301 GVVLLELITGRKAIDHSKPHSEQNLVAWARPLFRDRKKFSQMVDPMLQGHYPVRGLYQSL 360
           GVVLLELITGRKAID++K   +QNLV WARPLF+DR+ F +MVDP+LQG YPVRGLYQ+L
Sbjct: 301 GVVLLELITGRKAIDNTKTRKDQNLVGWARPLFKDRRNFPKMVDPLLQGQYPVRGLYQAL 360

Query: 361 AIAAMCVQEQPNMRPVITDVVTALNYLASQKYDPQTQPNQNSQKRPSSHKMERSDDKNET 420
           AI+AMCVQEQP MRPV++DVV ALN+LAS KYDP + P+ +S K PS H+ +R D++   
Sbjct: 361 AISAMCVQEQPTMRPVVSDVVLALNFLASSKYDPNS-PSSSSGKNPSFHR-DRDDEEKRP 420

Query: 421 SDNDESERE 422
               E+E E
Sbjct: 421 HLVKETECE 420

BLAST of Chy12G214910 vs. TAIR 10
Match: AT1G07870.2 (Protein kinase superfamily protein )

HSP 1 Score: 568.5 bits (1464), Expect = 4.3e-162
Identity = 302/429 (70.40%), Postives = 349/429 (81.35%), Query Frame = 0

Query: 1   MGCFRCTGSSSKKSEDIEQKDPN-------TFNSKTHKPDDRT-PSDKAKVISNLVKRDS 60
           MGCF C+  SSK+SE  +    N       T  +K+ K DD+T PS  +  +S    RD 
Sbjct: 1   MGCFGCSKKSSKRSETNKDTVINRKIVGGTTSVAKSDKRDDQTQPSSDSTKVSPY--RDV 60

Query: 61  DVKVVVSKNDQLALDVKKLNLKDEVSEEGEANGRAKTFTFEELAAATGNFRSDCFLGEGG 120
           + +  V K DQL+LDVK LNL D+V+ +     +A+TFTF+ELA ATGNFRSDCFLGEGG
Sbjct: 61  NNEGGVGKEDQLSLDVKGLNLNDQVTGK-----KAQTFTFQELAEATGNFRSDCFLGEGG 120

Query: 121 FGKVYKGYLEKVNEVVAIKQLDRNGLQGIREFVVEVLTLSLADHPNLVKLIGFCAEGDQR 180
           FGKV+KG +EK+++VVAIKQLDRNG+QGIREFVVEVLTLSLADHPNLVKLIGFCAEGDQR
Sbjct: 121 FGKVFKGTIEKLDQVVAIKQLDRNGVQGIREFVVEVLTLSLADHPNLVKLIGFCAEGDQR 180

Query: 181 LLVYEYMPLGSLENHLHDLRPGAKVIDWNTRMKIAAGAARGLEYLHEKMKPPVIYRDLKC 240
           LLVYEYMP GSLE+HLH L  G K +DWNTRMKIAAGAARGLEYLH++M PPVIYRDLKC
Sbjct: 181 LLVYEYMPQGSLEDHLHVLPSGKKPLDWNTRMKIAAGAARGLEYLHDRMTPPVIYRDLKC 240

Query: 241 SNILLGEGYHPKLSDFGLAKVGPSGDKTHVSTRVMGTYGYCAPDYAMTGQLTFKSDIYSF 300
           SNILLGE Y PKLSDFGLAKVGPSGDKTHVSTRVMGTYGYCAPDYAMTGQLTFKSDIYSF
Sbjct: 241 SNILLGEDYQPKLSDFGLAKVGPSGDKTHVSTRVMGTYGYCAPDYAMTGQLTFKSDIYSF 300

Query: 301 GVVLLELITGRKAIDHSKPHSEQNLVAWARPLFRDRKKFSQMVDPMLQGHYPVRGLYQSL 360
           GVVLLELITGRKAID++K   +QNLV WARPLF+DR+ F +MVDP+LQG YPVRGLYQ+L
Sbjct: 301 GVVLLELITGRKAIDNTKTRKDQNLVGWARPLFKDRRNFPKMVDPLLQGQYPVRGLYQAL 360

Query: 361 AIAAMCVQEQPNMRPVITDVVTALNYLASQKYDPQTQPNQNSQKRPSSHKMERSDDKNET 420
           AI+AMCVQEQP MRPV++DVV ALN+LAS KYDP + P+ +S K PS H+ +R D++   
Sbjct: 361 AISAMCVQEQPTMRPVVSDVVLALNFLASSKYDPNS-PSSSSGKNPSFHR-DRDDEEKRP 420

Query: 421 SDNDESERE 422
               E+E E
Sbjct: 421 HLVKETECE 420

BLAST of Chy12G214910 vs. TAIR 10
Match: AT2G28590.1 (Protein kinase superfamily protein )

HSP 1 Score: 536.6 bits (1381), Expect = 1.8e-152
Identity = 283/429 (65.97%), Postives = 335/429 (78.09%), Query Frame = 0

Query: 1   MGCFRCTGSSSKKSEDIEQKDPNTFNSKTHKPDDRTPSDKAKVISN---LVKRDSDV--- 60
           MGCF  T  S+K+S+           +KT K +D TP  K  V +N   L +  SD    
Sbjct: 1   MGCFGRTPKSNKRSD-----------TKTTKNNDFTPK-KLTVNANRDKLTQPSSDCLKV 60

Query: 61  -------KVVVSKNDQLALDVKKLNLKDEVSEEGEANGRAKTFTFEELAAATGNFRSDCF 120
                  K +V+K DQLALD K  N++DEV  +     +A+TFTFEEL+ +TGNF+SDCF
Sbjct: 61  SICGDVSKEIVTKKDQLALDAKDTNVEDEVIVK-----KAQTFTFEELSVSTGNFKSDCF 120

Query: 121 LGEGGFGKVYKGYLEKVNEVVAIKQLDRNGLQGIREFVVEVLTLSLADHPNLVKLIGFCA 180
           LGEGGFGKVYKG++EK+N+VVAIKQLDRNG QGIREFVVEVLTLSLADHPNLVKLIGFCA
Sbjct: 121 LGEGGFGKVYKGFIEKINQVVAIKQLDRNGAQGIREFVVEVLTLSLADHPNLVKLIGFCA 180

Query: 181 EGDQRLLVYEYMPLGSLENHLHDLRPGAKVIDWNTRMKIAAGAARGLEYLHEKMKPPVIY 240
           EG QRLLVYEYMPLGSL+NHLHDL  G   + WNTRMKIAAGAARGLEYLH+ MKPPVIY
Sbjct: 181 EGVQRLLVYEYMPLGSLDNHLHDLPSGKNPLAWNTRMKIAAGAARGLEYLHDTMKPPVIY 240

Query: 241 RDLKCSNILLGEGYHPKLSDFGLAKVGPSGDKTHVSTRVMGTYGYCAPDYAMTGQLTFKS 300
           RDLKCSNIL+ EGYH KLSDFGLAKVGP G +THVSTRVMGTYGYCAPDYA+TGQLTFKS
Sbjct: 241 RDLKCSNILIDEGYHAKLSDFGLAKVGPRGSETHVSTRVMGTYGYCAPDYALTGQLTFKS 300

Query: 301 DIYSFGVVLLELITGRKAIDHSKPHSEQNLVAWARPLFRDRKKFSQMVDPMLQGHYPVRG 360
           D+YSFGVVLLELITGRKA D+++  + Q+LV WA PLF+DRK F +MVDP+L+G YPVRG
Sbjct: 301 DVYSFGVVLLELITGRKAYDNTRTRNHQSLVEWANPLFKDRKNFKKMVDPLLEGDYPVRG 360

Query: 361 LYQSLAIAAMCVQEQPNMRPVITDVVTALNYLASQKYDPQTQPNQNSQKRPSSHKMERSD 417
           LYQ+LAIAAMCVQEQP+MRPVI DVV AL++LAS KYD       + QK+ +  + +  +
Sbjct: 361 LYQALAIAAMCVQEQPSMRPVIADVVMALDHLASSKYD-----RSHRQKQDNVTETKVDE 407

BLAST of Chy12G214910 vs. TAIR 10
Match: AT5G02800.1 (Protein kinase superfamily protein )

HSP 1 Score: 510.8 bits (1314), Expect = 1.1e-144
Identity = 251/358 (70.11%), Postives = 290/358 (81.01%), Query Frame = 0

Query: 46  NLVKRDSDVKVVVSKNDQLALDVKKLNLKDEVSE---EGEANGRAKTFTFEELAAATGNF 105
           N  +R+ D K+    +D      +K   K  +SE   +G  +  A+TFTF ELA AT NF
Sbjct: 14  NKTRRNGDHKLDRKSSDCSVSTSEKSRAKSSLSESKSKGSDHIVAQTFTFSELATATRNF 73

Query: 106 RSDCFLGEGGFGKVYKGYLEKVNEVVAIKQLDRNGLQGIREFVVEVLTLSLADHPNLVKL 165
           R +C +GEGGFG+VYKGYL   ++  AIKQLD NGLQG REF+VEVL LSL  HPNLV L
Sbjct: 74  RKECLIGEGGFGRVYKGYLASTSQTAAIKQLDHNGLQGNREFLVEVLMLSLLHHPNLVNL 133

Query: 166 IGFCAEGDQRLLVYEYMPLGSLENHLHDLRPGAKVIDWNTRMKIAAGAARGLEYLHEKMK 225
           IG+CA+GDQRLLVYEYMPLGSLE+HLHD+ PG + +DWNTRMKIAAGAA+GLEYLH+K  
Sbjct: 134 IGYCADGDQRLLVYEYMPLGSLEDHLHDISPGKQPLDWNTRMKIAAGAAKGLEYLHDKTM 193

Query: 226 PPVIYRDLKCSNILLGEGYHPKLSDFGLAKVGPSGDKTHVSTRVMGTYGYCAPDYAMTGQ 285
           PPVIYRDLKCSNILL + Y PKLSDFGLAK+GP GDK+HVSTRVMGTYGYCAP+YAMTGQ
Sbjct: 194 PPVIYRDLKCSNILLDDDYFPKLSDFGLAKLGPVGDKSHVSTRVMGTYGYCAPEYAMTGQ 253

Query: 286 LTFKSDIYSFGVVLLELITGRKAIDHSKPHSEQNLVAWARPLFRDRKKFSQMVDPMLQGH 345
           LT KSD+YSFGVVLLE+ITGRKAID S+   EQNLVAWARPLF+DR+KFSQM DPMLQG 
Sbjct: 254 LTLKSDVYSFGVVLLEIITGRKAIDSSRSTGEQNLVAWARPLFKDRRKFSQMADPMLQGQ 313

Query: 346 YPVRGLYQSLAIAAMCVQEQPNMRPVITDVVTALNYLASQKYDPQTQPNQNSQKRPSS 401
           YP RGLYQ+LA+AAMCVQEQPN+RP+I DVVTAL+YLASQK+DP  QP Q S   P +
Sbjct: 314 YPPRGLYQALAVAAMCVQEQPNLRPLIADVVTALSYLASQKFDPLAQPVQGSLFAPGT 371

BLAST of Chy12G214910 vs. TAIR 10
Match: AT5G18610.1 (Protein kinase superfamily protein )

HSP 1 Score: 490.0 bits (1260), Expect = 2.0e-138
Identity = 244/364 (67.03%), Postives = 285/364 (78.30%), Query Frame = 0

Query: 58  VSKNDQLALDVKKLNLKDEVSEE-------GEANGRAKTFTFEELAAATGNFRSDCFLGE 117
           V+++  ++LD  K     E  +E         A+  A+TFTF ELAAAT NFR +C LGE
Sbjct: 32  VTQSHHISLDKSKSRRGPEQKKELTAPKEGPTAHIAAQTFTFRELAAATKNFRPECLLGE 91

Query: 118 GGFGKVYKGYLEKVNEVVAIKQLDRNGLQGIREFVVEVLTLSLADHPNLVKLIGFCAEGD 177
           GGFG+VYKG LE   ++VA+KQLDRNGLQG REF+VEVL LSL  HPNLV LIG+CA+GD
Sbjct: 92  GGFGRVYKGRLETTGQIVAVKQLDRNGLQGNREFLVEVLMLSLLHHPNLVNLIGYCADGD 151

Query: 178 QRLLVYEYMPLGSLENHLHDLRPGAKVIDWNTRMKIAAGAARGLEYLHEKMKPPVIYRDL 237
           QRLLVYEYMPLGSLE+HLHDL P  + +DW+TRM IAAGAA+GLEYLH+K  PPVIYRDL
Sbjct: 152 QRLLVYEYMPLGSLEDHLHDLPPDKEPLDWSTRMTIAAGAAKGLEYLHDKANPPVIYRDL 211

Query: 238 KCSNILLGEGYHPKLSDFGLAKVGPSGDKTHVSTRVMGTYGYCAPDYAMTGQLTFKSDIY 297
           K SNILLG+GYHPKLSDFGLAK+GP GDKTHVSTRVMGTYGYCAP+YAMTGQLT KSD+Y
Sbjct: 212 KSSNILLGDGYHPKLSDFGLAKLGPVGDKTHVSTRVMGTYGYCAPEYAMTGQLTLKSDVY 271

Query: 298 SFGVVLLELITGRKAIDHSKPHSEQNLVAWARPLFRDRKKFSQMVDPMLQGHYPVRGLYQ 357
           SFGVV LELITGRKAID+++   E NLVAWARPLF+DR+KF +M DP LQG YP+RGLYQ
Sbjct: 272 SFGVVFLELITGRKAIDNARAPGEHNLVAWARPLFKDRRKFPKMADPSLQGRYPMRGLYQ 331

Query: 358 SLAIAAMCVQEQPNMRPVITDVVTALNYLASQKYDPQTQPNQNSQKRPSSHKMERSDDKN 415
           +LA+AAMC+QEQ   RP+I DVVTAL YLASQ +DP     QNS+       +   DD+ 
Sbjct: 332 ALAVAAMCLQEQAATRPLIGDVVTALTYLASQTFDPNAPSGQNSRSGSGPPFIRTRDDRR 391

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LQQ86.1e-16170.40Probable serine/threonine-protein kinase PBL5 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q9SIB62.6e-15165.97Probable serine/threonine-protein kinase PBL6 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q0WRY51.5e-14370.11Probable serine/threonine-protein kinase PBL7 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q1PDV62.8e-13767.03Serine/threonine-protein kinase PBL27 OS=Arabidopsis thaliana OX=3702 GN=PBL27 P... [more]
Q6I5Q62.8e-13764.04Receptor-like cytoplasmic kinase 185 OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
Match NameE-valueIdentityDescription
A0A0A0LSG43.8e-23899.05Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G077... [more]
A0A5D3DCD24.6e-23698.34Putative serine/threonine-protein kinase RLCKVII OS=Cucumis melo var. makuwa OX=... [more]
A0A1S3CNI74.6e-23698.34probable serine/threonine-protein kinase RLCKVII OS=Cucumis melo OX=3656 GN=LOC1... [more]
A0A6J1I3P01.3e-22293.36probable serine/threonine-protein kinase PBL5 OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A6J1HJX54.2e-22192.65probable serine/threonine-protein kinase PBL5 OS=Cucurbita moschata OX=3662 GN=L... [more]
Match NameE-valueIdentityDescription
XP_004148777.19.45e-30299.05probable serine/threonine-protein kinase PBL5 [Cucumis sativus] >KGN64723.1 hypo... [more]
XP_008465289.15.24e-29998.34PREDICTED: probable serine/threonine-protein kinase RLCKVII [Cucumis melo] >TYK2... [more]
XP_038895614.12.43e-28694.79probable serine/threonine-protein kinase PBL5 [Benincasa hispida][more]
XP_022970373.12.59e-28193.36probable serine/threonine-protein kinase PBL5 [Cucurbita maxima][more]
XP_023519827.16.09e-28092.89probable serine/threonine-protein kinase PBL5 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT1G07870.14.3e-16270.40Protein kinase superfamily protein [more]
AT1G07870.24.3e-16270.40Protein kinase superfamily protein [more]
AT2G28590.11.8e-15265.97Protein kinase superfamily protein [more]
AT5G02800.11.1e-14470.11Protein kinase superfamily protein [more]
AT5G18610.12.0e-13867.03Protein kinase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 102..379
e-value: 6.9E-40
score: 148.5
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 107..372
e-value: 5.1E-44
score: 150.6
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 102..379
score: 36.640289
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 179..408
e-value: 2.9E-60
score: 205.1
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 70..178
e-value: 1.2E-31
score: 110.9
NoneNo IPR availablePIRSRPIRSR630220-2PIRSR630220-2coord: 108..301
e-value: 1.1E-16
score: 58.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 11..40
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 383..421
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..40
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 398..421
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 383..397
NoneNo IPR availablePANTHERPTHR47985:SF41SERINE/THREONINE-PROTEIN KINASE PBL5-RELATEDcoord: 1..410
NoneNo IPR availablePANTHERPTHR47985OS07G0668900 PROTEINcoord: 1..410
NoneNo IPR availableCDDcd14066STKc_IRAKcoord: 108..377
e-value: 7.38908E-96
score: 286.862
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 108..131
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 225..237
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 88..400

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy12G214910.1Chy12G214910.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity