Chy12G214520 (gene) Cucumber (hystrix) v1

Overview


Name	Chy12G214520
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	DNA-directed RNA polymerases I and III subunit RPAC2
Location	chrH12: 13756268 .. 13759856 (-)
RNA-Seq Expression	Chy12G214520
Synteny	Chy12G214520

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGAAGGTTTCAAATGCAAGAAGAAAAAGCAAAAGGGTATGGAGGTACACCGTTAATTAAGCTGAAACAGAGTGAGAATGGGTAAAATTTGAAGGTTGAGAAGGAAAGTTATAGAAAGAAGGAGATAGCTAGAGAGTACCTGAAGTGAAAAGGGTGAGAGTGTGAAGGTTGAAGAAGTTGAGACGGTTGTTAATGGAAGCAATTGAGGAAGAAAGATAGAGAGGAAGAGTGATCACAGATCACAGAAAAGAGGAACCGCACCCGAGAGAGAAGAACAAATTAATTATCCTTAGAACAGAAAAGATCATGCAAAATTTGAAAATACACTTATTTCTAAAATACATAAAAAAAAAAATTAAAATTAGGGTGTCGGCAAAAATGAAAAGAAAAAATTATATCACTATATTTTTTAAATTATAAAAGTAACAAATTTAAAAGCATACAACTCTCCGATAGTTCTTTGATATCATCAATCAATTGTCATATTTATCATATCTGCAATATGTATAAAAAAATGTGTTATGCACTGTTTTTTTCTAATTTTTTTTGTCCTATAATGCAATTTCTCTTAAAATTAAGCTAGCTTTGATGGTGAAAATTCCACTACAAAACAAATGGTCCAACGACATTCATGTCTTTATTTTTTATTTTTTCATTTTTTTTTATCTATAGAAACTTTTGTATTTCTTTTTCTGTTTTTTCAATTTGTACACTTATTTTCTTATCTTCAAGGAACAAACAAAAATTTATCTTGTAAAATTAAGAATCAAAACATACCAAAAATAAATAGTCAAATAAAAAAAATAATTTAACTGTCAACATACATGTACATATTTGTTAAAATATCTCTAAATACATTCAATATTTTCATTTTTAAACATGTGTGTTCCCTTCTACGTGCAAACAATATGTTATTTAATTTCTATTTACCAAACTCAACATGCCACAAATTTGGCTGATTTTGTAATATATTATTTCTTTAATTATCATATAGTTCTAAATTAGCTAACACTTTCTAAATTCTAATTTGTAAATAATAACAATAACAATAAATCTATTTACACTTAATAACAAAAAAAAAAAAAAAAAACATTACACATTCATAATTAAAAAAAATGGTTAAGATTAGAAGATTTGAGAAAATATTTATAATAAATTTTGTTTATAAATAGTTTGCTAATTTTTGTGAAAAAACTCCTAAAATAATTGTTCTTAATTTTTTTTTTTAAATTACAACCTTGCTGTAATCTCTCTTCCTGCTCCACTACAGCCCAGCCGCAGCCCCTCTCTTCGGCGAGCTTCTTCGAACCGCATACGAACGGTGGCCACAACACCGTATCGCCCAAGAGCAGCGTCCGGTGACCTCAGTGGCGGACTACACCCGACACGTTTGCTTGGTCGACAGCTTGGAGCAACTGTGAGTGACGCCACCAACGTCTTCTGGCTTTTTCAGCGGCGTTTTCTCTAGTTCAAGGAGTTTCGGTACTTGCCCATTCTATTTTAACATTAGATTTTGAGTTACCCACTTCTTAACGGCTCCAAACATGTAGGATTGAACGTATTCGGACGCCAACCAGCAAAGTTTCAAGTCGTTTGGATAAGTTTCTGCGGGGTAAGAATGGTCTAAATTTCCTTTTGAACTTGTTTAAGGTCCTTTATTTAGACACTTTTCACCCTTTTGGATTAGATTTAGATGGTGTAAATTAGTTTATAACTCTTGGTATGCTTAATTTGTGGTTTAAGGTTAGAGACTTTTGAGGAGGATAATTGAAGAATCATGAAACTAGTCTGCCTAATTTTATCTGTTAACTTCTGATGATTGATATTTCAATTCTATAAAGTTGGTGGAGAAAAGATTTTAACTTCTTGGATCCGTTGGTTATGTAGAATTTTGGCATTTTTTTTATGTTATCAAATTTTTGGTTTATTTTCTTTGATATTCTCAATTTATTTACCTTCATGCTAATCAAGATGGTATTTTGTTTGGTTTTCCATTTTTTAAATAGCAATGGAACATGGATCCTTCACAGATGAGACCAAATCAACATTTTCTGTGGCTGATGAAGATCATACACTTGCCAATACTCTCAGATACACTTTGAACCAAGAGTACGAGCTCTTATTCAGTTATTCTTGAGTGAATATTTCGAAATGTTAACATGACATGGACATGCCAAAGAATTTGCAGTTCTTGGACTACTTATCTTTTCTAACCATTTCATCACTGATTTCTATTAATTCATGACATAAATAATTAAACAGATTCTTGGTTCTTGTGAACATATCATCAACAAGGTCCTTGAAAAGTTAATTAGGATCTAGCTGGTTTCGATTATTGTTATTTATTCTTTAGTAAATCATATTCAGACATACTTTTAAATGACTTGCAATGATCTTGCAGTCCAAGAGTGACATTCTGTGGATACAGCATCCCTCATCCTTCAGATAATCGAGTTAATATTCGAGTTCAGACAACAGGTAATTTGTCTCTTTAAAAGTGGAGCCGATTGGTTCCCATGCGCAGGCTCGACTTAATACATAGTAGCGGACATGTTTTCTAATGATTAGATTTGTTCTTTCTGTTTTTATGCTTTATACACATCCAATCTATGCATCCATGAGATATATAGCTTATGGATCTATGGGAAGTGTGATCCTCTTTAAAGAAAAATCTTTCAGTAAAATCACCTTGTTGCATTGCTAGAACCCCGTTATTTTTATAAGCACATAAATATATATTTTAGTATTCATATGAAAGGTTGTATCTATTTAGTTTCATAACTTTAACGAGATTCAATTGTCTATTTAAAATTTGAATAGTTAACTATCATGGATTGGTGTACTGGTTAAAAAGGAGATATAGTCTCAACAGCTAATTAAGATGTCATGGGTTCAATCCATGGTCGCTACCTGCCTTTTTCTACAACTTTTCTTGGCACTTTGGACATACCACACTATTGTCTAATCAACAATAATGTTAATAACTATGACTACACATGACTATACATGATTAATTACACTTGCAAAGTGTGCAAACCACATTTAAAACTTGAAATACCAATTAGGCACAACCATTTTAGTTACTTTCGAGTTTTAAGGGCATAGTTGTACAACTTATAGAATAGTTCAAATCCAAATAGGGTCATAAATGTATAATTTAAACATGAACATATGTGCATATATTGTTTTGCAACTGTGAAGTAAACCCAACCAACCAAGATGTCGAGGCCTCACAAATATAAATAGTGTCCCAAATAAAGGATTCTTACTCTTCCCCCATAGTTGGAGTTCCATTATTGATTACTAGGGAAAACAAAGCACATGGAAATATGGTAGGAGCAATATAGCAAACAGAAGACTATCTGTTGAAGTGACTATGGATTATTAGCATTACTGAAAGAAAGGTTAATTATCGTTGAATTAAGCTGGATTTTTTATTGAACATGGTAGGTGATCCTGCGAGAGAGGTTTTGAAAGATGCTTGTCAGAACTTGATGGTGGTGTGCCAACATGTGAGGAACGCATTTGACAAAGCTGTGCTTGATGTCAAACTCACCAACCCTGAGCGAGACATTAATATCAAATAG

mRNA sequence

ATGGGAAGGTTTCAAATGCAAGAAGAAAAAGCAAAAGGGTATGGAGGTACACCGTTAATTAAGCTGAAACAGAGTGAGAATGGCCCAGCCGCAGCCCCTCTCTTCGGCGAGCTTCTTCGAACCGCATACGAACGGTGGCCACAACACCGTATCGCCCAAGAGCAGCGTCCGGTGACCTCAGTGGCGGACTACACCCGACACGTTTGCTTGGTCGACAGCTTGGAGCAACTGATTGAACGTATTCGGACGCCAACCAGCAAAGTTTCAAGTCGTTTGGATAAGTTTCTGCGGGCAATGGAACATGGATCCTTCACAGATGAGACCAAATCAACATTTTCTGTGGCTGATGAAGATCATACACTTGCCAATACTCTCAGATACACTTTGAACCAAGATCCAAGAGTGACATTCTGTGGATACAGCATCCCTCATCCTTCAGATAATCGAGTTAATATTCGAGTTCAGACAACAGGTGATCCTGCGAGAGAGGTTTTGAAAGATGCTTGTCAGAACTTGATGGTGGTGTGCCAACATGTGAGGAACGCATTTGACAAAGCTGTGCTTGATGTCAAACTCACCAACCCTGAGCGAGACATTAATATCAAATAG

Coding sequence (CDS)

ATGGGAAGGTTTCAAATGCAAGAAGAAAAAGCAAAAGGGTATGGAGGTACACCGTTAATTAAGCTGAAACAGAGTGAGAATGGCCCAGCCGCAGCCCCTCTCTTCGGCGAGCTTCTTCGAACCGCATACGAACGGTGGCCACAACACCGTATCGCCCAAGAGCAGCGTCCGGTGACCTCAGTGGCGGACTACACCCGACACGTTTGCTTGGTCGACAGCTTGGAGCAACTGATTGAACGTATTCGGACGCCAACCAGCAAAGTTTCAAGTCGTTTGGATAAGTTTCTGCGGGCAATGGAACATGGATCCTTCACAGATGAGACCAAATCAACATTTTCTGTGGCTGATGAAGATCATACACTTGCCAATACTCTCAGATACACTTTGAACCAAGATCCAAGAGTGACATTCTGTGGATACAGCATCCCTCATCCTTCAGATAATCGAGTTAATATTCGAGTTCAGACAACAGGTGATCCTGCGAGAGAGGTTTTGAAAGATGCTTGTCAGAACTTGATGGTGGTGTGCCAACATGTGAGGAACGCATTTGACAAAGCTGTGCTTGATGTCAAACTCACCAACCCTGAGCGAGACATTAATATCAAATAG

Protein sequence

MGRFQMQEEKAKGYGGTPLIKLKQSENGPAAAPLFGELLRTAYERWPQHRIAQEQRPVTSVADYTRHVCLVDSLEQLIERIRTPTSKVSSRLDKFLRAMEHGSFTDETKSTFSVADEDHTLANTLRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTGDPAREVLKDACQNLMVVCQHVRNAFDKAVLDVKLTNPERDINIK*

Homology

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BLAST of Chy12G214520 vs. ExPASy Swiss-Prot
Match: Q5XK67 (DNA-directed RNA polymerases I and III subunit RPAC2 OS=Xenopus laevis OX=8355 GN=polr1d PE=3 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 3.4e-16
Identity = 40/87 (45.98%), Postives = 58/87 (66.67%), Query Frame = 0


Query: 106 DETKSTFSVADEDHTLANTLRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQT-TGDPAREV 165
           +E+  TF + +EDHTL N+LRY + ++P V FCGYSI HPS+ ++N R+QT  G PA E 
Sbjct: 20  EESCVTFVLHEEDHTLGNSLRYMVMKNPEVEFCGYSITHPSETKINFRIQTRNGIPAVEP 79

Query: 166 LKDACQNLMVVCQHVRNAFDKAVLDVK 192
            +     L+ VCQHV N F+ ++ + K
Sbjct: 80  FRRGLNELVDVCQHVLNTFETSIKEYK 106

[Close]

BLAST of Chy12G214520 vs. ExPASy Swiss-Prot
Match: P0DPB6 (DNA-directed RNA polymerases I and III subunit RPAC2 OS=Homo sapiens OX=9606 GN=POLR1D PE=1 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 4.4e-16
Identity = 41/94 (43.62%), Postives = 60/94 (63.83%), Query Frame = 0


Query: 99  MEHGSFTDETKSTFSVADEDHTLANTLRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 158
           M   + TD    TF + +EDHTL N+LRY + ++P V FCGY+  HPS++++N+R+QT G
Sbjct: 29  MVQAAGTDRHCVTFVLHEEDHTLGNSLRYMIMKNPEVEFCGYTTTHPSESKINLRIQTRG 88

Query: 159 D-PAREVLKDACQNLMVVCQHVRNAFDKAVLDVK 192
             PA E  +     LM VCQHV + F+ ++ D K
Sbjct: 89  TLPAVEPFQRGLNELMNVCQHVLDKFEASIKDYK 122

[Close]

BLAST of Chy12G214520 vs. ExPASy Swiss-Prot
Match: P28000 (DNA-directed RNA polymerases I and III subunit RPAC2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=RPC19 PE=1 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 1.3e-15
Identity = 38/93 (40.86%), Postives = 61/93 (65.59%), Query Frame = 0


Query: 96  LRAMEHGSFTDETKSTFSVADEDHTLANTLRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQ 155
           ++ +   +  D T ++F + +EDHTL N LRY + ++P V FCGYSIPHPS+N +NIR+Q
Sbjct: 47  IKLLTQATSEDGTSASFQIVEEDHTLGNALRYVIMKNPDVEFCGYSIPHPSENLLNIRIQ 106

Query: 156 TTGD-PAREVLKDACQNLMVVCQHVRNAFDKAV 188
           T G+  A + L+   ++LM +C  V + F + +
Sbjct: 107 TYGETTAVDALQKGLKDLMDLCDVVESKFTEKI 139

[Close]

BLAST of Chy12G214520 vs. ExPASy Swiss-Prot
Match: Q1RMG8 (DNA-directed RNA polymerases I and III subunit RPAC2 OS=Bos taurus OX=9913 GN=POLR1D PE=2 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 1.7e-15
Identity = 40/94 (42.55%), Postives = 61/94 (64.89%), Query Frame = 0


Query: 99  MEHGSFTDETKSTFSVADEDHTLANTLRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 158
           M   + TD    TF + +EDHTL N+LRY + ++P V FCGY+  HPS++++N+R+QT G
Sbjct: 29  MVQAAGTDRHCVTFVLHEEDHTLGNSLRYMIMKNPEVEFCGYTTTHPSESKINLRIQTRG 88

Query: 159 D-PAREVLKDACQNLMVVCQHVRNAFDKAVLDVK 192
             PA E  +    +LM VCQHV + F+ ++ + K
Sbjct: 89  ALPAVEPFQRGLTDLMNVCQHVLDKFEASIKEYK 122

[Close]

BLAST of Chy12G214520 vs. ExPASy Swiss-Prot
Match: P97304 (DNA-directed RNA polymerases I and III subunit RPAC2 OS=Mus musculus OX=10090 GN=Polr1d PE=1 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 3.8e-15
Identity = 40/94 (42.55%), Postives = 58/94 (61.70%), Query Frame = 0


Query: 99  MEHGSFTDETKSTFSVADEDHTLANTLRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 158
           M   + TD    TF + +EDHTL N LRY + ++P V FCGY+  HPS++++N+R+QT G
Sbjct: 29  MVQAAGTDRQCVTFVLHEEDHTLGNCLRYIIMKNPEVEFCGYTTTHPSESKINLRIQTRG 88

Query: 159 D-PAREVLKDACQNLMVVCQHVRNAFDKAVLDVK 192
             PA E  +     L+ VCQHV   F+ ++ D K
Sbjct: 89  ALPAVEPFQKGLNELLNVCQHVLVKFEASIKDYK 122

[Close]

BLAST of Chy12G214520 vs. ExPASy TrEMBL
Match: A0A0A0LUT5 (RNA_pol_L_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G095010 PE=3 SV=1)

HSP 1 Score: 213.4 bits (542), Expect = 8.9e-52
Identity = 102/104 (98.08%), Postives = 102/104 (98.08%), Query Frame = 0


Query: 99  MEHGSFTDETKSTFSVADEDHTLANTLRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 158
           MEHGSFTDETKSTFSVADEDHTLANTLRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG
Sbjct: 1   MEHGSFTDETKSTFSVADEDHTLANTLRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 60

Query: 159 DPAREVLKDACQNLMVVCQHVRNAFDKAVLDVKLTNPERDINIK 203
           DPAREVLKDACQNLMVVCQHVRN FDKAVLDVKLTNP RDINIK
Sbjct: 61  DPAREVLKDACQNLMVVCQHVRNTFDKAVLDVKLTNPVRDINIK 104

[Close]

BLAST of Chy12G214520 vs. ExPASy TrEMBL
Match: A0A1S3C5G1 (DNA-directed RNA polymerases I and III subunit RPAC2 OS=Cucumis melo OX=3656 GN=LOC103497108 PE=3 SV=1)

HSP 1 Score: 196.8 bits (499), Expect = 8.6e-47
Identity = 96/108 (88.89%), Postives = 98/108 (90.74%), Query Frame = 0


Query: 99  MEHGSFTDETKSTFSVADEDHTLANTLRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 158
           MEHGSFTDETKSTFS+ADEDHTLAN LRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG
Sbjct: 1   MEHGSFTDETKSTFSMADEDHTLANALRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 60

Query: 159 DPAREVLKDACQNLMVVCQHVRNAFDKAVLDVKLT----NPERDINIK 203
           DPAREVLKDACQNLMVVCQHVRN FDKAVLD  LT     PE DINI+
Sbjct: 61  DPAREVLKDACQNLMVVCQHVRNTFDKAVLDFNLTKAKEEPEGDINIE 108

[Close]

BLAST of Chy12G214520 vs. ExPASy TrEMBL
Match: A0A6J1KDK3 (DNA-directed RNA polymerases I and III subunit RPAC2 OS=Cucurbita maxima OX=3661 GN=LOC111493371 PE=3 SV=1)

HSP 1 Score: 196.8 bits (499), Expect = 8.6e-47
Identity = 93/104 (89.42%), Postives = 97/104 (93.27%), Query Frame = 0


Query: 99  MEHGSFTDETKSTFSVADEDHTLANTLRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 158
           MEHGSFTDE+KSTFS+ADEDHTLAN++RYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG
Sbjct: 1   MEHGSFTDESKSTFSMADEDHTLANSIRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 60

Query: 159 DPAREVLKDACQNLMVVCQHVRNAFDKAVLDVKLTNPERDINIK 203
           DPAREVLKDACQNLM VCQHVRN  DKAVLD KLT P  DINIK
Sbjct: 61  DPAREVLKDACQNLMAVCQHVRNTLDKAVLDHKLTKPVGDINIK 104

[Close]

BLAST of Chy12G214520 vs. ExPASy TrEMBL
Match: A0A5D3BBZ7 (DNA-directed RNA polymerases I and III subunit RPAC2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold194G001620 PE=3 SV=1)

HSP 1 Score: 196.8 bits (499), Expect = 8.6e-47
Identity = 96/108 (88.89%), Postives = 98/108 (90.74%), Query Frame = 0


Query: 99  MEHGSFTDETKSTFSVADEDHTLANTLRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 158
           MEHGSFTDETKSTFS+ADEDHTLAN LRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG
Sbjct: 1   MEHGSFTDETKSTFSMADEDHTLANALRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 60

Query: 159 DPAREVLKDACQNLMVVCQHVRNAFDKAVLDVKLT----NPERDINIK 203
           DPAREVLKDACQNLMVVCQHVRN FDKAVLD  LT     PE DINI+
Sbjct: 61  DPAREVLKDACQNLMVVCQHVRNTFDKAVLDFNLTKAKEEPEGDINIE 108

[Close]

BLAST of Chy12G214520 vs. ExPASy TrEMBL
Match: A0A6J1G901 (DNA-directed RNA polymerases I and III subunit RPAC2 OS=Cucurbita moschata OX=3662 GN=LOC111452057 PE=3 SV=1)

HSP 1 Score: 196.8 bits (499), Expect = 8.6e-47
Identity = 93/104 (89.42%), Postives = 97/104 (93.27%), Query Frame = 0


Query: 99  MEHGSFTDETKSTFSVADEDHTLANTLRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 158
           MEHGSFTDE+KSTFS+ADEDHTLAN++RYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG
Sbjct: 1   MEHGSFTDESKSTFSMADEDHTLANSIRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 60

Query: 159 DPAREVLKDACQNLMVVCQHVRNAFDKAVLDVKLTNPERDINIK 203
           DPAREVLKDACQNLM VCQHVRN  DKAVLD KLT P  DINIK
Sbjct: 61  DPAREVLKDACQNLMAVCQHVRNTLDKAVLDHKLTKPVGDINIK 104

[Close]

BLAST of Chy12G214520 vs. NCBI nr
Match: KAE8652742.1 (hypothetical protein Csa_014245 [Cucumis sativus])

HSP 1 Score: 236 bits (601), Expect = 1.57e-75
Identity = 121/156 (77.56%), Postives = 126/156 (80.77%), Query Frame = 0


Query: 57  PVTSVADYTRHVCLVDSLEQL----------IERIRTPTSKVSSRLDKFLRAMEHGSFTD 116
           P ++VADYTRHVCL +SLEQL          +       +K       F  AMEHGSFTD
Sbjct: 45  PKSTVADYTRHVCLANSLEQLTVFSSSRSFGLNVFGRQPAKFQVVWISFCGAMEHGSFTD 104

Query: 117 ETKSTFSVADEDHTLANTLRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTGDPAREVLK 176
           ETKSTFSVADEDHTLANTLRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTGDPAREVLK
Sbjct: 105 ETKSTFSVADEDHTLANTLRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTGDPAREVLK 164

Query: 177 DACQNLMVVCQHVRNAFDKAVLDVKLTNPERDINIK 202
           DACQNLMVVCQHVRN FDKAVLDVKLTNP RDINIK
Sbjct: 165 DACQNLMVVCQHVRNTFDKAVLDVKLTNPVRDINIK 200

[Close]

BLAST of Chy12G214520 vs. NCBI nr
Match: XP_004147276.2 (DNA-directed RNA polymerases I and III subunit RPAC2 [Cucumis sativus] >XP_011653666.1 DNA-directed RNA polymerases I and III subunit RPAC2 [Cucumis sativus])

HSP 1 Score: 212 bits (540), Expect = 1.25e-67
Identity = 102/104 (98.08%), Postives = 102/104 (98.08%), Query Frame = 0


Query: 99  MEHGSFTDETKSTFSVADEDHTLANTLRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 158
           MEHGSFTDETKSTFSVADEDHTLANTLRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG
Sbjct: 1   MEHGSFTDETKSTFSVADEDHTLANTLRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 60

Query: 159 DPAREVLKDACQNLMVVCQHVRNAFDKAVLDVKLTNPERDINIK 202
           DPAREVLKDACQNLMVVCQHVRN FDKAVLDVKLTNP RDINIK
Sbjct: 61  DPAREVLKDACQNLMVVCQHVRNTFDKAVLDVKLTNPVRDINIK 104

[Close]

BLAST of Chy12G214520 vs. NCBI nr
Match: XP_038893936.1 (DNA-directed RNA polymerases I and III subunit RPAC2 [Benincasa hispida])

HSP 1 Score: 204 bits (518), Expect = 2.78e-64
Identity = 97/104 (93.27%), Postives = 99/104 (95.19%), Query Frame = 0


Query: 99  MEHGSFTDETKSTFSVADEDHTLANTLRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 158
           MEHGSFTDETKSTFS+ADEDHTLAN LRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG
Sbjct: 1   MEHGSFTDETKSTFSMADEDHTLANALRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 60

Query: 159 DPAREVLKDACQNLMVVCQHVRNAFDKAVLDVKLTNPERDINIK 202
           DPAREVLKDACQNLMVVCQHVRN FDKAVLD+KLT P  DINIK
Sbjct: 61  DPAREVLKDACQNLMVVCQHVRNTFDKAVLDIKLTKPVGDINIK 104

[Close]

BLAST of Chy12G214520 vs. NCBI nr
Match: KAG7036964.1 (DNA-directed RNA polymerases I and III subunit RPAC2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 200 bits (508), Expect = 5.14e-62
Identity = 96/111 (86.49%), Postives = 101/111 (90.99%), Query Frame = 0


Query: 92  LDKFLRAMEHGSFTDETKSTFSVADEDHTLANTLRYTLNQDPRVTFCGYSIPHPSDNRVN 151
           L  F+ AMEHGSFTDE+KSTFS+ADEDHTLAN++RYTLNQDPRVTFCGYSIPHPSDNRVN
Sbjct: 47  LPLFVTAMEHGSFTDESKSTFSMADEDHTLANSIRYTLNQDPRVTFCGYSIPHPSDNRVN 106

Query: 152 IRVQTTGDPAREVLKDACQNLMVVCQHVRNAFDKAVLDVKLTNPERDINIK 202
           IRVQTTGDPAREVLKDACQNLM VCQHVRN  DKAVLD KLT P  DINIK
Sbjct: 107 IRVQTTGDPAREVLKDACQNLMAVCQHVRNTLDKAVLDHKLTKPVGDINIK 157

[Close]

BLAST of Chy12G214520 vs. NCBI nr
Match: XP_023522054.1 (DNA-directed RNA polymerases I and III subunit RPAC2-like [Cucurbita pepo subsp. pepo] >XP_023524948.1 DNA-directed RNA polymerases I and III subunit RPAC2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 197 bits (501), Expect = 1.07e-61
Identity = 94/104 (90.38%), Postives = 97/104 (93.27%), Query Frame = 0


Query: 99  MEHGSFTDETKSTFSVADEDHTLANTLRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 158
           MEHGSFTDETKSTFS+ADEDHTLAN++RYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG
Sbjct: 1   MEHGSFTDETKSTFSMADEDHTLANSIRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 60

Query: 159 DPAREVLKDACQNLMVVCQHVRNAFDKAVLDVKLTNPERDINIK 202
           DPAREVLKDACQNLM VCQHVRN  DKAVLD KLT P  DINIK
Sbjct: 61  DPAREVLKDACQNLMAVCQHVRNTLDKAVLDHKLTKPVGDINIK 104

[Close]

BLAST of Chy12G214520 vs. TAIR 10
Match: AT2G29540.1 (RNApolymerase 14 kDa subunit )

HSP 1 Score: 132.9 bits (333), Expect = 2.9e-31
Identity = 61/93 (65.59%), Postives = 77/93 (82.80%), Query Frame = 0


Query: 99  MEHGSFTDETKSTFSVADEDHTLANTLRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 158
           MEHGSFT+ + ++F++++EDHTLAN +R+ LNQDPRVT   Y+IPHPS  +VNIRVQTTG
Sbjct: 1   MEHGSFTNVSHASFTLSEEDHTLANAVRFVLNQDPRVTVAAYTIPHPSLEQVNIRVQTTG 60

Query: 159 DPAREVLKDACQNLMVVCQHVRNAFDKAVLDVK 192
           DPAREV KDACQ LM + +HVR+ FDKAV + K
Sbjct: 61  DPAREVFKDACQELMQMNRHVRSVFDKAVAEYK 93

[Close]

BLAST of Chy12G214520 vs. TAIR 10
Match: AT2G29540.2 (RNApolymerase 14 kDa subunit )

HSP 1 Score: 132.9 bits (333), Expect = 2.9e-31
Identity = 61/93 (65.59%), Postives = 77/93 (82.80%), Query Frame = 0


Query: 99  MEHGSFTDETKSTFSVADEDHTLANTLRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 158
           MEHGSFT+ + ++F++++EDHTLAN +R+ LNQDPRVT   Y+IPHPS  +VNIRVQTTG
Sbjct: 1   MEHGSFTNVSHASFTLSEEDHTLANAVRFVLNQDPRVTVAAYTIPHPSLEQVNIRVQTTG 60

Query: 159 DPAREVLKDACQNLMVVCQHVRNAFDKAVLDVK 192
           DPAREV KDACQ LM + +HVR+ FDKAV + K
Sbjct: 61  DPAREVFKDACQELMQMNRHVRSVFDKAVAEYK 93

[Close]

BLAST of Chy12G214520 vs. TAIR 10
Match: AT2G29540.3 (RNApolymerase 14 kDa subunit )

HSP 1 Score: 88.2 bits (217), Expect = 8.3e-18
Identity = 41/59 (69.49%), Postives = 47/59 (79.66%), Query Frame = 0


Query: 133 PRVTFCGYSIPHPSDNRVNIRVQTTGDPAREVLKDACQNLMVVCQHVRNAFDKAVLDVK 192
           PRVT   Y+IPHPS  +VNIRVQTTGDPAREV KDACQ LM + +HVR+ FDKAV + K
Sbjct: 52  PRVTVAAYTIPHPSLEQVNIRVQTTGDPAREVFKDACQELMQMNRHVRSVFDKAVAEYK 110

[Close]

BLAST of Chy12G214520 vs. TAIR 10
Match: AT3G52090.1 (DNA-directed RNA polymerase, RBP11-like )

HSP 1 Score: 54.7 bits (130), Expect = 1.0e-07
Identity = 28/90 (31.11%), Postives = 49/90 (54.44%), Query Frame = 0


Query: 103 SFTDETK----STFSVADEDHTLANTLRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 162
           S+  +TK    ++F+V  EDHT+ N +R  L++D  V F GY +PHP   ++ +R+ TT 
Sbjct: 20  SYDRDTKIINAASFTVEREDHTIGNIVRMQLHRDENVLFAGYQLPHPLKYKIIVRIHTTS 79

Query: 163 DPA-REVLKDACQNLMVVCQHVRNAFDKAV 188
             +  +    A  +L     +++N F+  V
Sbjct: 80  QSSPMQAYNQAINDLDKELDYLKNQFEAEV 109

[Close]

BLAST of Chy12G214520 vs. TAIR 10
Match: AT3G52090.2 (DNA-directed RNA polymerase, RBP11-like )

HSP 1 Score: 47.8 bits (112), Expect = 1.2e-05
Identity = 29/77 (37.66%), Postives = 43/77 (55.84%), Query Frame = 0


Query: 103 SFTDETK----STFSVADEDHTLANTLRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 162
           S+  +TK    ++F+V  EDHT+ N +R  L++D  V F GY +PHP   ++ +R     
Sbjct: 20  SYDRDTKIINAASFTVEREDHTIGNIVRMQLHRDENVLFAGYQLPHPLKYKIIVR----- 79

Query: 163 DPAREVLKDACQNLMVV 176
           DP     KD  +NL VV
Sbjct: 80  DP-----KDWFRNLKVV 86

The following BLAST results are available for this feature:

BLAST of Chy12G214520 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q5XK67	3.4e-16	45.98	DNA-directed RNA polymerases I and III subunit RPAC2 OS=Xenopus laevis OX=8355 G...	[more]
P0DPB6	4.4e-16	43.62	DNA-directed RNA polymerases I and III subunit RPAC2 OS=Homo sapiens OX=9606 GN=...	[more]
P28000	1.3e-15	40.86	DNA-directed RNA polymerases I and III subunit RPAC2 OS=Saccharomyces cerevisiae...	[more]
Q1RMG8	1.7e-15	42.55	DNA-directed RNA polymerases I and III subunit RPAC2 OS=Bos taurus OX=9913 GN=PO...	[more]
P97304	3.8e-15	42.55	DNA-directed RNA polymerases I and III subunit RPAC2 OS=Mus musculus OX=10090 GN...	[more]

BLAST of Chy12G214520 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LUT5	8.9e-52	98.08	RNA_pol_L_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G095010...	[more]
A0A1S3C5G1	8.6e-47	88.89	DNA-directed RNA polymerases I and III subunit RPAC2 OS=Cucumis melo OX=3656 GN=...	[more]
A0A6J1KDK3	8.6e-47	89.42	DNA-directed RNA polymerases I and III subunit RPAC2 OS=Cucurbita maxima OX=3661...	[more]
A0A5D3BBZ7	8.6e-47	88.89	DNA-directed RNA polymerases I and III subunit RPAC2 OS=Cucumis melo var. makuwa...	[more]
A0A6J1G901	8.6e-47	89.42	DNA-directed RNA polymerases I and III subunit RPAC2 OS=Cucurbita moschata OX=36...	[more]

BLAST of Chy12G214520 vs. NCBI nr


Match Name	E-value	Identity	Description
KAE8652742.1	1.57e-75	77.56	hypothetical protein Csa_014245 [Cucumis sativus]	[more]
XP_004147276.2	1.25e-67	98.08	DNA-directed RNA polymerases I and III subunit RPAC2 [Cucumis sativus] >XP_01165...	[more]
XP_038893936.1	2.78e-64	93.27	DNA-directed RNA polymerases I and III subunit RPAC2 [Benincasa hispida]	[more]
KAG7036964.1	5.14e-62	86.49	DNA-directed RNA polymerases I and III subunit RPAC2, partial [Cucurbita argyros...	[more]
XP_023522054.1	1.07e-61	90.38	DNA-directed RNA polymerases I and III subunit RPAC2-like [Cucurbita pepo subsp....	[more]

BLAST of Chy12G214520 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G29540.1	2.9e-31	65.59	RNApolymerase 14 kDa subunit	[more]
AT2G29540.2	2.9e-31	65.59	RNApolymerase 14 kDa subunit	[more]
AT2G29540.3	8.3e-18	69.49	RNApolymerase 14 kDa subunit	[more]
AT3G52090.1	1.0e-07	31.11	DNA-directed RNA polymerase, RBP11-like	[more]
AT3G52090.2	1.2e-05	37.66	DNA-directed RNA polymerase, RBP11-like	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036603	RNA polymerase, RBP11-like subunit	GENE3D	3.30.1360.10		coord: 97..193 e-value: 2.5E-33 score: 116.1
IPR036603	RNA polymerase, RBP11-like subunit	SUPERFAMILY	55257	RBP11-like subunits of RNA polymerase	coord: 106..188
IPR009025	DNA-directed RNA polymerase, RBP11-like dimerisation domain	PFAM	PF13656	RNA_pol_L_2	coord: 110..181 e-value: 2.2E-28 score: 97.8
None	No IPR available	PANTHER	PTHR13946	DNA-DIRECTED RNA POLYMERASE I,II,III	coord: 99..202
None	No IPR available	PANTHER	PTHR13946:SF37	SUBFAMILY NOT NAMED	coord: 99..202
IPR008193	DNA-directed RNA polymerase Rpb11, 13-16kDa subunit, conserved site	PROSITE	PS01154	RNA_POL_L_13KD	coord: 114..145
IPR033898	DNA-directed RNA polymerases I and III subunit AC19	CDD	cd07029	RNAP_I_III_AC19	coord: 100..183 e-value: 4.3375E-43 score: 137.363

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy12G214520.1	Chy12G214520.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006351	transcription, DNA-templated
cellular_component	GO:0005736	RNA polymerase I complex
cellular_component	GO:0005666	RNA polymerase III complex
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003899	DNA-directed 5'-3' RNA polymerase activity
molecular_function	GO:0046983	protein dimerization activity