Chy12G214420 (gene) Cucumber (hystrix) v1

BLAST of Chy12G214420 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 3.2e-10
Identity = 57/204 (27.94%), Postives = 94/204 (46.08%), Query Frame = 0

Query: 164 YLATFSGSGSALAAIDNLGQVAESLAYPSDAISIIISWVSVFNFFGRIFSGFISENIMMK 223
           Y+A F G    L   +NLGQ+A+SL   S   + +++  S F+FFGR+ S    + +  +
Sbjct: 364 YIAYFCGGTIGLVYSNNLGQIAQSLGQNS---TTLVTIYSSFSFFGRLLSA-APDFMHKR 423

Query: 224 WKLPRPLTFAVPFFITGIGQLIVAYPSTGSVFI--ASMVIGFGFGMQVPLLFAIISDLFG 283
           ++L R   FA+    T I   ++A  S+    +  A+ +IG   G       +I SDLFG
Sbjct: 424 FRLTRTGWFAIALLPTPIAFFLLAVSSSQQTALQTATALIGLSSGFIFAAAVSITSDLFG 483

Query: 284 LKHYSTLFNCGQLVVPIGSYILNVHVVGRIYDKEALVNGIKLTGRGLICTGAHCFNLCFT 343
                   N     +PIGS +L  ++   IY+  A  +   +    ++C G  C+   F 
Sbjct: 484 PNSVGVNHNILITNIPIGS-LLYGYIAASIYEANASPDITPIVSDSIVCIGRDCYFKTFV 543

Query: 344 ILAGATLFGGIIMLVLAYRTRDFY 366
                ++ G +  L L  RT+  Y
Sbjct: 544 FWGCLSILGVVSSLSLYIRTKPVY 562

BLAST of Chy12G214420 vs. ExPASy TrEMBL
Match: A0A5A7UCC0 (Protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2273G00090 PE=4 SV=1)

HSP 1 Score: 652.1 bits (1681), Expect = 1.5e-183
Identity = 359/566 (63.43%), Postives = 378/566 (66.78%), Query Frame = 0

Query: 1   MGTKTWDFTKQVIQGRWFSVFAGLIMMLGNGSTYIYGTYSKVIKTEFNYSQTQLSILGFA 60
           MGTK WD+TKQVIQGRWFSVFAGLIMM+GNGSTYIYGTYSKVIKTEFNYSQTQLSILGFA
Sbjct: 1   MGTKAWDYTKQVIQGRWFSVFAGLIMMVGNGSTYIYGTYSKVIKTEFNYSQTQLSILGFA 60

Query: 61  KDLGSNVGIFAGLLAEVAPPWVLFLIGSVQNFTGFFLIWLSITHRIPQPR--QIFLCVCL 120
           KDLGSNVGIFAGLLAEVAPPWVLFLIGSVQNFTGFFLIWLSITHRIPQP+  Q+FLCVCL
Sbjct: 61  KDLGSNVGIFAGLLAEVAPPWVLFLIGSVQNFTGFFLIWLSITHRIPQPKFWQMFLCVCL 120

Query: 121 GTNSSNFANKTIMATSVVNFPDRHGIILGLLKGYVGIGGATLTQIY-------------- 180
           GT+SSNFAN  IM TSVVNFPDR GIILGLL GYVGIGGA LTQIY              
Sbjct: 121 GTHSSNFANTAIMVTSVVNFPDRRGIILGLLNGYVGIGGAILTQIYLGIYGPKDPSNLVL 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 LFAWLPSAVILVLSFSIRPICIHKHPEELKVFYHLLYVSVILAMFILFLTIAQKEVAFTR 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 RGYTNGAAVIVVLLFLPLVIVCREEHVLYKLNKQNEDSSFNISINDHQKPHSSITTEKTE 300

Query: 301 ----------------------------------LATFSGSGSALAAIDNLGQVAESLAY 360
                                             LATFSGSGSAL AIDNLGQVAESLAY
Sbjct: 301 NSRSYFSKIWNKPERGEDFSILQAIFSIDMTLIFLATFSGSGSALTAIDNLGQVAESLAY 360

Query: 361 PSDAISIIISWVSVFNFFGRIFSGFISENIMMKWKLPRPLTFAVPFFITGIGQLIVAYPS 397
           PS+AISII+SWVSVFNFFGRIFSGFISEN M+K+KLPRPLTFAV FFITGIGQLIVAYPS
Sbjct: 361 PSEAISIIVSWVSVFNFFGRIFSGFISENFMIKYKLPRPLTFAVAFFITGIGQLIVAYPS 420

BLAST of Chy12G214420 vs. ExPASy TrEMBL
Match: A0A1S3CM93 (uncharacterized protein LOC103502588 OS=Cucumis melo OX=3656 GN=LOC103502588 PE=4 SV=1)

HSP 1 Score: 652.1 bits (1681), Expect = 1.5e-183
Identity = 359/566 (63.43%), Postives = 378/566 (66.78%), Query Frame = 0

Query: 1   MGTKTWDFTKQVIQGRWFSVFAGLIMMLGNGSTYIYGTYSKVIKTEFNYSQTQLSILGFA 60
           MGTK WD+TKQVIQGRWFSVFAGLIMM+GNGSTYIYGTYSKVIKTEFNYSQTQLSILGFA
Sbjct: 1   MGTKAWDYTKQVIQGRWFSVFAGLIMMVGNGSTYIYGTYSKVIKTEFNYSQTQLSILGFA 60

Query: 61  KDLGSNVGIFAGLLAEVAPPWVLFLIGSVQNFTGFFLIWLSITHRIPQPR--QIFLCVCL 120
           KDLGSNVGIFAGLLAEVAPPWVLFLIGSVQNFTGFFLIWLSITHRIPQP+  Q+FLCVCL
Sbjct: 61  KDLGSNVGIFAGLLAEVAPPWVLFLIGSVQNFTGFFLIWLSITHRIPQPKFWQMFLCVCL 120

Query: 121 GTNSSNFANKTIMATSVVNFPDRHGIILGLLKGYVGIGGATLTQIY-------------- 180
           GT+SSNFAN  IM TSVVNFPDR GIILGLL GYVGIGGA LTQIY              
Sbjct: 121 GTHSSNFANTAIMVTSVVNFPDRRGIILGLLNGYVGIGGAILTQIYLGIYGPKDPSNLVL 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 LFAWLPSAVILVLSFSIRPICIHKHPEELKVFYHLLYVSVILAMFILFLTIAQKEVAFTR 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 RGYTNGAAVIVVLLFLPLVIVCREEHVLYKLNKQNEDSSFNISINDHQKPHSSITTEKTE 300

Query: 301 ----------------------------------LATFSGSGSALAAIDNLGQVAESLAY 360
                                             LATFSGSGSAL AIDNLGQVAESLAY
Sbjct: 301 NSRSYFSKIWNKPERGEDFSILQAIFSIDMTLIFLATFSGSGSALTAIDNLGQVAESLAY 360

Query: 361 PSDAISIIISWVSVFNFFGRIFSGFISENIMMKWKLPRPLTFAVPFFITGIGQLIVAYPS 397
           PS+AISII+SWVSVFNFFGRIFSGFISEN M+K+KLPRPLTFAV FFITGIGQLIVAYPS
Sbjct: 361 PSEAISIIVSWVSVFNFFGRIFSGFISENFMIKYKLPRPLTFAVAFFITGIGQLIVAYPS 420

BLAST of Chy12G214420 vs. ExPASy TrEMBL
Match: A0A5A7U9N3 (Protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2273G00150 PE=4 SV=1)

HSP 1 Score: 527.7 bits (1358), Expect = 4.2e-146
Identity = 267/459 (58.17%), Postives = 325/459 (70.81%), Query Frame = 0

Query: 1   MGTKTWDFTKQVIQGRWFSVFAGLIMMLGNGSTYIYGTYSKVIKTEFNYSQTQLSILGFA 60
           M ++ W F KQV+QGRWFSV+AG++MM+GNG+TYI+GTYSKV+KTEF+YSQTQ+++LGFA
Sbjct: 1   MESEAWAFIKQVVQGRWFSVYAGMMMMIGNGTTYIFGTYSKVLKTEFDYSQTQVNMLGFA 60

Query: 61  KDLGSNVGIFAGLLAEVAPPWVLFLIGSVQNFTGFFLIWLSITHRIPQPR--QIFLCVCL 120
           KDLGSNVGI AGLLAE  P WVLF IG+ QNFT +F IWLS+T RIPQP   Q+FL VC 
Sbjct: 61  KDLGSNVGIIAGLLAEFVPTWVLFTIGAFQNFTCYFFIWLSMTRRIPQPEFWQMFLSVCF 120

Query: 121 GTNSSNFANKTIMATSVVNFPDRHGIILGLLKG--------------------------- 180
           G+NSSN+AN  IM  S+ NFPDR GIILGLLKG                           
Sbjct: 121 GSNSSNYANTAIMVISLRNFPDRRGIILGLLKGEEFLLYKLKKQNLNRDPSVALSIVDQK 180

Query: 181 -------------------------------------YVGIGGATLTQIYLATFSGSGSA 240
                                                   I    +  I LATFSG GS+
Sbjct: 181 VTNCPKSFSTLEEIAEISPSCISNICNKPDRGNDFTVLQAIFSIDMLLICLATFSGCGSS 240

Query: 241 LAAIDNLGQVAESLAYPSDAISIIISWVSVFNFFGRIFSGFISENIMMKWKLPRPLTFAV 300
           LAAIDNLGQ+ ESL YP+ AISI +SWVS+FNFFGR+FSGFISE +++K+KLPRPL FA 
Sbjct: 241 LAAIDNLGQIGESLGYPARAISIFVSWVSIFNFFGRVFSGFISELMIIKYKLPRPLMFAF 300

Query: 301 PFFITGIGQLIVAYPSTGSVFIASMVIGFGFGMQVPLLFAIISDLFGLKHYSTLFNCGQL 360
            F +T +GQL +AYP  GSV++AS +IGFGFG Q PLLFA+IS++FGLKHYS LFNCGQL
Sbjct: 301 AFLLTCLGQLCIAYPFPGSVYVASSIIGFGFGAQNPLLFAVISEMFGLKHYSILFNCGQL 360

Query: 361 VVPIGSYILNVHVVGRIYDKEALVNGIKLTGRGLICTGAHCFNLCFTILAGATLFGGIIM 394
           VVP+GSYILNV +VG++YD EAL  G K+TG+G+ CTGAHCF   FTILA +TLFG ++M
Sbjct: 361 VVPLGSYILNVDIVGKLYDAEALREGRKMTGKGITCTGAHCFGGSFTILAASTLFGALVM 420

BLAST of Chy12G214420 vs. ExPASy TrEMBL
Match: A0A6J1I2B3 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111469248 PE=4 SV=1)

HSP 1 Score: 515.0 bits (1325), Expect = 2.8e-142
Identity = 279/564 (49.47%), Postives = 334/564 (59.22%), Query Frame = 0

Query: 2   GTKTWDFTKQVIQGRWFSVFAGLIMMLGNGSTYIYGTYSKVIKTEFNYSQTQLSILGFAK 61
           G++ W F KQV+ GRWFSVFAGLIMM+GNG+TY++ TYSKVIKT+F+YSQTQ++ LGFAK
Sbjct: 6   GSEAWGFIKQVVTGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINTLGFAK 65

Query: 62  DLGSNVGIFAGLLAEVAPPWVLFLIGSVQNFTGFFLIWLSITHRIPQPR--QIFLCVCLG 121
           DLGSNVGI AGLLAEV P WVLF++G+ QNFTGFFLIWLSIT RI +P+  Q+F+ VC G
Sbjct: 66  DLGSNVGIIAGLLAEVTPTWVLFIVGAFQNFTGFFLIWLSITARIAKPKFWQMFILVCFG 125

Query: 122 TNSSNFANKTIMATSVVNFPDRHGIILGLLKGYVGIGGATLTQIY--------------- 181
           TNSSN+AN  IM TSV NFPDR GIILGLLKGYVGIGGA ++Q+Y               
Sbjct: 126 TNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIMSQLYLAIYGHNDPSNLVLL 185

Query: 182 ------------------------------------------------------------ 241
                                                                       
Sbjct: 186 FAWLPSLLILILFPSIRPIRIRKHPNELKVFYQLLYVSILLAIFILFLTIAQKQVVFSQA 245

Query: 242 ------------------------------------------------------------ 301
                                                                       
Sbjct: 246 GYVGGASFVVSLLCVPILIACREELLLYKLNKQTAGPSVTVSMVHQNPPTLSASADSEIP 305

Query: 302 -----------------------------------LATFSGSGSALAAIDNLGQVAESLA 361
                                              LATFSG GS+LAAIDNLGQV ESL 
Sbjct: 306 EILPSCCENIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNLGQVGESLG 365

Query: 362 YPSDAISIIISWVSVFNFFGRIFSGFISENIMMKWKLPRPLTFAVPFFITGIGQLIVAYP 394
           YP  AI I++SWVS+FNFFGR+FSGFISE +M K+KLPRPLTF+  F IT +GQL +AYP
Sbjct: 366 YPQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFSFAFLITCVGQLFIAYP 425

BLAST of Chy12G214420 vs. ExPASy TrEMBL
Match: A0A6J1I5D7 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111469398 PE=4 SV=1)

HSP 1 Score: 511.1 bits (1315), Expect = 4.0e-141
Identity = 278/564 (49.29%), Postives = 332/564 (58.87%), Query Frame = 0

Query: 2   GTKTWDFTKQVIQGRWFSVFAGLIMMLGNGSTYIYGTYSKVIKTEFNYSQTQLSILGFAK 61
           G++ W F KQV+ GRWFSVFAGLIMM+GNG+TY++ TYSKVIKT+F+YSQTQ++ LGFAK
Sbjct: 6   GSEAWGFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINTLGFAK 65

Query: 62  DLGSNVGIFAGLLAEVAPPWVLFLIGSVQNFTGFFLIWLSITHRIPQPR--QIFLCVCLG 121
           DLGSN+GI AGLLAEV P WVLF+IG+ QNF GFFLIWLSIT RI +P+  Q+F+ VC G
Sbjct: 66  DLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFIGFFLIWLSITARIAKPKFWQMFILVCFG 125

Query: 122 TNSSNFANKTIMATSVVNFPDRHGIILGLLKGYVGIGGATLTQIY--------------- 181
           TNSSN+AN  IM TSV NFPDR GIILGLLKGYVGIGGA ++Q Y               
Sbjct: 126 TNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIMSQFYLAIYGHNDPSNLVLL 185

Query: 182 ------------------------------------------------------------ 241
                                                                       
Sbjct: 186 FAWLPSLLILILFPSIRPIRIRKHPNELKVFYQLLYVSILLAIFILFLTIAQKQVVFSQA 245

Query: 242 ------------------------------------------------------------ 301
                                                                       
Sbjct: 246 GYVGGASFVVSLLCVPILIACREELLLYKLNKQTAGPSVTVSMVHQNPPTLPASADSEIP 305

Query: 302 -----------------------------------LATFSGSGSALAAIDNLGQVAESLA 361
                                              LATFSG GS+LAAIDNLGQV ESL 
Sbjct: 306 EISPSCCENIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNLGQVGESLG 365

Query: 362 YPSDAISIIISWVSVFNFFGRIFSGFISENIMMKWKLPRPLTFAVPFFITGIGQLIVAYP 394
           YP  AI I++SWVS+FNFFGR+FSGFISE +M K+KLPRPLTF+  F IT +GQL +AYP
Sbjct: 366 YPQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFSFAFLITCVGQLFIAYP 425

BLAST of Chy12G214420 vs. NCBI nr
Match: XP_008464784.1 (PREDICTED: uncharacterized protein LOC103502588 [Cucumis melo] >KAA0051886.1 protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis melo var. makuwa] >TYK17148.1 protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis melo var. makuwa])

HSP 1 Score: 654 bits (1688), Expect = 5.84e-232
Identity = 359/566 (63.43%), Postives = 378/566 (66.78%), Query Frame = 0

Query: 1   MGTKTWDFTKQVIQGRWFSVFAGLIMMLGNGSTYIYGTYSKVIKTEFNYSQTQLSILGFA 60
           MGTK WD+TKQVIQGRWFSVFAGLIMM+GNGSTYIYGTYSKVIKTEFNYSQTQLSILGFA
Sbjct: 1   MGTKAWDYTKQVIQGRWFSVFAGLIMMVGNGSTYIYGTYSKVIKTEFNYSQTQLSILGFA 60

Query: 61  KDLGSNVGIFAGLLAEVAPPWVLFLIGSVQNFTGFFLIWLSITHRIPQPR--QIFLCVCL 120
           KDLGSNVGIFAGLLAEVAPPWVLFLIGSVQNFTGFFLIWLSITHRIPQP+  Q+FLCVCL
Sbjct: 61  KDLGSNVGIFAGLLAEVAPPWVLFLIGSVQNFTGFFLIWLSITHRIPQPKFWQMFLCVCL 120

Query: 121 GTNSSNFANKTIMATSVVNFPDRHGIILGLLKGYVGIGGATLTQIYL------------- 180
           GT+SSNFAN  IM TSVVNFPDR GIILGLL GYVGIGGA LTQIYL             
Sbjct: 121 GTHSSNFANTAIMVTSVVNFPDRRGIILGLLNGYVGIGGAILTQIYLGIYGPKDPSNLVL 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 LFAWLPSAVILVLSFSIRPICIHKHPEELKVFYHLLYVSVILAMFILFLTIAQKEVAFTR 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 RGYTNGAAVIVVLLFLPLVIVCREEHVLYKLNKQNEDSSFNISINDHQKPHSSITTEKTE 300

Query: 301 -----------------------------------ATFSGSGSALAAIDNLGQVAESLAY 360
                                              ATFSGSGSAL AIDNLGQVAESLAY
Sbjct: 301 NSRSYFSKIWNKPERGEDFSILQAIFSIDMTLIFLATFSGSGSALTAIDNLGQVAESLAY 360

Query: 361 PSDAISIIISWVSVFNFFGRIFSGFISENIMMKWKLPRPLTFAVPFFITGIGQLIVAYPS 396
           PS+AISII+SWVSVFNFFGRIFSGFISEN M+K+KLPRPLTFAV FFITGIGQLIVAYPS
Sbjct: 361 PSEAISIIVSWVSVFNFFGRIFSGFISENFMIKYKLPRPLTFAVAFFITGIGQLIVAYPS 420

BLAST of Chy12G214420 vs. NCBI nr
Match: XP_038895748.1 (uncharacterized protein LOC120083912 [Benincasa hispida])

HSP 1 Score: 570 bits (1468), Expect = 6.80e-197
Identity = 309/562 (54.98%), Postives = 350/562 (62.28%), Query Frame = 0

Query: 1   MGTKTWDFTKQVIQGRWFSVFAGLIMMLGNGSTYIYGTYSKVIKTEFNYSQTQLSILGFA 60
           M +K W FTKQV+ GRWFSVFAGL+MMLGNGSTYIYGTYSKVIKT+F+YSQTQ++ILGFA
Sbjct: 118 MDSKAWSFTKQVVAGRWFSVFAGLMMMLGNGSTYIYGTYSKVIKTQFDYSQTQVNILGFA 177

Query: 61  KDLGSNVGIFAGLLAEVAPPWVLFLIGSVQNFTGFFLIWLSITHRIPQPR--QIFLCVCL 120
           KDLGSNVGIFAGLL EVAP WVLFLIGS  NFTGFF+IWL+ITHRIPQP+  Q+F  VC 
Sbjct: 178 KDLGSNVGIFAGLLGEVAPAWVLFLIGSFLNFTGFFMIWLAITHRIPQPKFWQMFCLVCF 237

Query: 121 GTNSSNFANKTIMATSVVNFPDRHGIILGLLKGYVGIGGATLTQIYL------------- 180
           GTNSSNFAN  IM +SV NFPDR GIILGLLKG+VGIGGA  TQIYL             
Sbjct: 238 GTNSSNFANTAIMVSSVGNFPDRRGIILGLLKGFVGIGGAVYTQIYLGIYGHDDPANLVL 297

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 298 LFAWLPSTVTLLLFTSIRPIRIRKHPEELKVFYHLLYVSIIIALFILFLTLAQKEVVFSR 357

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 358 SGYGSGATVIVALLFLPLLIACREEYLLYKLNRDDDASVTLSINEQKQPPPVTTTEISQS 417

Query: 301 -------------------------------ATFSGSGSALAAIDNLGQVAESLAYPSDA 360
                                          ATF+GSGS+LAAIDNLGQV ESL YP  A
Sbjct: 418 CCSGIWNKPERGEDFSILQAIFSIDMALIYLATFAGSGSSLAAIDNLGQVGESLGYPPRA 477

Query: 361 ISIIISWVSVFNFFGRIFSGFISENIMMKWKLPRPLTFAVPFFITGIGQLIVAYPSTGSV 396
           ISI +SWVS+FNFFGR+FSGF+SE +M+K+KLPRP+ FAV F IT IGQL +AYP+ GSV
Sbjct: 478 ISIFVSWVSIFNFFGRVFSGFVSETMMIKYKLPRPVMFAVAFLITCIGQLFIAYPTPGSV 537

BLAST of Chy12G214420 vs. NCBI nr
Match: KAA0051880.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis melo var. makuwa] >TYK17152.1 protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis melo var. makuwa])

HSP 1 Score: 530 bits (1366), Expect = 1.32e-184
Identity = 268/462 (58.01%), Postives = 326/462 (70.56%), Query Frame = 0

Query: 1   MGTKTWDFTKQVIQGRWFSVFAGLIMMLGNGSTYIYGTYSKVIKTEFNYSQTQLSILGFA 60
           M ++ W F KQV+QGRWFSV+AG++MM+GNG+TYI+GTYSKV+KTEF+YSQTQ+++LGFA
Sbjct: 1   MESEAWAFIKQVVQGRWFSVYAGMMMMIGNGTTYIFGTYSKVLKTEFDYSQTQVNMLGFA 60

Query: 61  KDLGSNVGIFAGLLAEVAPPWVLFLIGSVQNFTGFFLIWLSITHRIPQPR--QIFLCVCL 120
           KDLGSNVGI AGLLAE  P WVLF IG+ QNFT +F IWLS+T RIPQP   Q+FL VC 
Sbjct: 61  KDLGSNVGIIAGLLAEFVPTWVLFTIGAFQNFTCYFFIWLSMTRRIPQPEFWQMFLSVCF 120

Query: 121 GTNSSNFANKTIMATSVVNFPDRHGIILGLLKG--------------------------- 180
           G+NSSN+AN  IM  S+ NFPDR GIILGLLKG                           
Sbjct: 121 GSNSSNYANTAIMVISLRNFPDRRGIILGLLKGEEFLLYKLKKQNLNRDPSVALSIVDQK 180

Query: 181 -------------------------------------YVGIGGATLTQIYLATFSGSGSA 240
                                                   I    +  I LATFSG GS+
Sbjct: 181 VTNCPKSFSTLEEIAEISPSCISNICNKPDRGNDFTVLQAIFSIDMLLICLATFSGCGSS 240

Query: 241 LAAIDNLGQVAESLAYPSDAISIIISWVSVFNFFGRIFSGFISENIMMKWKLPRPLTFAV 300
           LAAIDNLGQ+ ESL YP+ AISI +SWVS+FNFFGR+FSGFISE +++K+KLPRPL FA 
Sbjct: 241 LAAIDNLGQIGESLGYPARAISIFVSWVSIFNFFGRVFSGFISELMIIKYKLPRPLMFAF 300

Query: 301 PFFITGIGQLIVAYPSTGSVFIASMVIGFGFGMQVPLLFAIISDLFGLKHYSTLFNCGQL 360
            F +T +GQL +AYP  GSV++AS +IGFGFG Q PLLFA+IS++FGLKHYS LFNCGQL
Sbjct: 301 AFLLTCLGQLCIAYPFPGSVYVASSIIGFGFGAQNPLLFAVISEMFGLKHYSILFNCGQL 360

Query: 361 VVPIGSYILNVHVVGRIYDKEALVNGIKLTGRGLICTGAHCFNLCFTILAGATLFGGIIM 396
           VVP+GSYILNV +VG++YD EAL  G K+TG+G+ CTGAHCF   FTILA +TLFG ++M
Sbjct: 361 VVPLGSYILNVDIVGKLYDAEALREGRKMTGKGITCTGAHCFGGSFTILAASTLFGALVM 420

BLAST of Chy12G214420 vs. NCBI nr
Match: KAG6583522.1 (Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 531 bits (1368), Expect = 3.73e-184
Identity = 276/504 (54.76%), Postives = 330/504 (65.48%), Query Frame = 0

Query: 2   GTKTWDFTKQVIQGRWFSVFAGLIMMLGNGSTYIYGTYSKVIKTEFNYSQTQLSILGFAK 61
           G++ W F KQV+ GRWFSVFAGLIMM+GNG+TY++ TYSKVIKT+F+YSQTQ++ LGFAK
Sbjct: 6   GSEAWAFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINTLGFAK 65

Query: 62  DLGSNVGIFAGLLAEVAPPWVLFLIGSVQNFTGFFLIWLSITHRIPQPR--QIFLCVCLG 121
           DLGSN+GI AGLLAEV P WVLF+IG+ QNFTGFFLIWLSIT RI +P+  Q+F+ VCLG
Sbjct: 66  DLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFTGFFLIWLSITARIAKPKFWQMFILVCLG 125

Query: 122 TNSSNFANKTIMATSVVNFPDRHGIILGLLKGYVGIGGATLTQIYLA------------- 181
           TNSSN+AN  IM TSV NFPDR GIILGLLKGYVGIGGA ++Q+YLA             
Sbjct: 126 TNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIISQLYLAIYGHNDPSNLVLL 185

Query: 182 ------------------------------------------------------------ 241
                                                                       
Sbjct: 186 FAWLPSLLILILFPSIRPIRIQKSCCSTNSTNKPLLLLSHSPCFTKNPPTLPTSADSEIP 245

Query: 242 -------------------------------------TFSGSGSALAAIDNLGQVAESLA 301
                                                TFSG GS+LAAIDNLGQV ESL 
Sbjct: 246 EISPSCCPNIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNLGQVGESLG 305

Query: 302 YPSDAISIIISWVSVFNFFGRIFSGFISENIMMKWKLPRPLTFAVPFFITGIGQLIVAYP 361
           Y   AI I++SWVS+FNFFGR+FSGFISE +M K+KLPRPLTFA  F IT +GQL +AYP
Sbjct: 306 YSQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFAFAFLITCVGQLFIAYP 365

Query: 362 STGSVFIASMVIGFGFGMQVPLLFAIISDLFGLKHYSTLFNCGQLVVPIGSYILNVHVVG 393
             GS+++A+++IGFGFG Q P+LFA+IS++FGLK YSTLFNCGQL  P GSYILNV VVG
Sbjct: 366 FPGSLYLAAIIIGFGFGAQNPMLFAVISEMFGLKRYSTLFNCGQLAAPFGSYILNVDVVG 425

BLAST of Chy12G214420 vs. NCBI nr
Match: KAG6583528.1 (Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 529 bits (1362), Expect = 5.35e-184
Identity = 268/454 (59.03%), Postives = 321/454 (70.70%), Query Frame = 0

Query: 2   GTKTWDFTKQVIQGRWFSVFAGLIMMLGNGSTYIYGTYSKVIKTEFNYSQTQLSILGFAK 61
           G++ W F KQV+ GRWFSVFAGLIMM+GNG+TY++ TYSKVIKT+F+YSQTQ++ LGFAK
Sbjct: 6   GSEAWAFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINTLGFAK 65

Query: 62  DLGSNVGIFAGLLAEVAPPWVLFLIGSVQNFTGFFLIWLSITHRIPQPR--QIFLCVCLG 121
           DLGSN+GI AGLLAEV P WVLF+IG+ QNFTGFFLIWLSIT RI +P+  Q+F+ VCLG
Sbjct: 66  DLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFTGFFLIWLSITARIAKPKFWQMFILVCLG 125

Query: 122 TNSSNFANKTIMATSVVNFPDRHGIILGLLKG---------------------------- 181
           TNSSN+AN  IM TSV NFPDR GIILGLLKG                            
Sbjct: 126 TNSSNWANTAIMVTSVRNFPDRRGIILGLLKGEELLLYKLNKQTAAPSVALSMLHQNPPT 185

Query: 182 --------------------------------YVGIGGATLTQIYLATFSGSGSALAAID 241
                                              +    +  I LATFSG GS+LAAID
Sbjct: 186 LPTSADSEIPEISPSCCPNIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAID 245

Query: 242 NLGQVAESLAYPSDAISIIISWVSVFNFFGRIFSGFISENIMMKWKLPRPLTFAVPFFIT 301
           NLGQV ESL Y   AI I++SWVS+FNFFGR+FSGFISE +M K+KLPRPLTFA  F IT
Sbjct: 246 NLGQVGESLGYSQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFAFAFLIT 305

Query: 302 GIGQLIVAYPSTGSVFIASMVIGFGFGMQVPLLFAIISDLFGLKHYSTLFNCGQLVVPIG 361
            +GQL +AYP  GS+++A+++IGFGFG Q P+LFA+IS++FGLK YSTLFNCGQL  P G
Sbjct: 306 CVGQLFIAYPFPGSLYLAAIIIGFGFGAQNPMLFAVISEMFGLKRYSTLFNCGQLAAPFG 365

Query: 362 SYILNVHVVGRIYDKEALVNGIKLTGRGLICTGAHCFNLCFTILAGATLFGGIIMLVLAY 393
           SYILNV VVG++YD EAL    ++ G+GL CTGAHCF   FT+LA ATLFG ++M VLAY
Sbjct: 366 SYILNVDVVGKLYDMEALRERKQIAGKGLTCTGAHCFGGSFTVLAAATLFGAVVMFVLAY 425

BLAST of Chy12G214420 vs. TAIR 10
Match: AT2G28120.1 (Major facilitator superfamily protein )

HSP 1 Score: 357.5 bits (916), Expect = 1.4e-98
Identity = 212/574 (36.93%), Postives = 285/574 (49.65%), Query Frame = 0

Query: 4   KTWDFTKQVIQGRWFSVFAGLIMMLGNGSTYIYGTYSKVIKTEFNYSQTQLSILGFAKDL 63
           +T  F      GRWF VFA  ++M   G+TY++GTYSK IK+   Y QT L++LGF KDL
Sbjct: 5   ETVRFLIHFFNGRWFMVFASFLIMACAGATYLFGTYSKDIKSTLGYDQTTLNLLGFFKDL 64

Query: 64  GSNVGIFAGLLAEVAPPWVLFLIGSVQNFTGFFLIWLSITHRIPQPR--QIFLCVCLGTN 123
           G+NVG+ +GL+AEV P W +  IGS  NF G+F+IWL++T ++ +P+  Q+ L +C+G N
Sbjct: 65  GANVGVLSGLIAEVTPTWFVLTIGSAMNFVGYFMIWLTVTGKVAKPKVWQMCLYICIGAN 124

Query: 124 SSNFANKTIMATSVVNFPDRHGIILGLLKGYVGIGGATLTQIY----------------- 183
           S NFAN   + T V NFP+  G++LGLLKGYVG+ GA  TQ+Y                 
Sbjct: 125 SQNFANTGALVTCVKNFPESRGVMLGLLKGYVGLSGAIFTQLYFAIYGHDSKSLILLIAW 184

Query: 184 ------------------------------------------------------------ 243
                                                                       
Sbjct: 185 LPAAVSLVFVYLIREKKVVRQRNELSVFYQFLYISIFLALFLMAMNIAEKQVHFSKAAYA 244

Query: 244 ------------------------------------------------------------ 303
                                                                       
Sbjct: 245 ASATICCALLFVPLTVSVKQELEVWNMMKLPIEEPSEVKVEKPKKELDLDQDKAAKVNGE 304

Query: 304 ------------------------------------LATFSGSGSALAAIDNLGQVAESL 363
                                               +ATF G GS+L A+DNLGQ+ ESL
Sbjct: 305 EKETKSCFSTVFSPPPRGEDYTILQALLSTDMIILFVATFCGLGSSLTAVDNLGQIGESL 364

Query: 364 AYPSDAISIIISWVSVFNFFGRIFSGFISENIMMKWKLPRPLTFAVPFFITGIGQLIVAY 395
            YP+  +S  +S VS++N+FGR+FSGF+SE ++ K+KLPRPL   +   ++  G L++A+
Sbjct: 365 GYPNHTVSSFVSLVSIWNYFGRVFSGFVSEYLLAKYKLPRPLMMTLVLLLSCAGHLLIAF 424

BLAST of Chy12G214420 vs. TAIR 10
Match: AT2G39210.1 (Major facilitator superfamily protein )

HSP 1 Score: 313.2 bits (801), Expect = 3.1e-85
Identity = 186/561 (33.16%), Postives = 261/561 (46.52%), Query Frame = 0

Query: 9   TKQVIQGRWFSVFAGLIMMLGNGSTYIYGTYSKVIKTEFNYSQTQLSILGFAKDLGSNVG 68
           T Q++ GRWF  F  L++M   G+TY++G YS  IK    Y QT L++L F KDLG+NVG
Sbjct: 14  TIQILTGRWFMFFGSLLIMSTAGATYMFGIYSGDIKETLGYDQTTLNLLSFFKDLGANVG 73

Query: 69  IFAGLLAEVAPPWVLFLIGSVQNFTGFFLIWLSITHRIPQPRQIFLC--VCLGTNSSNFA 128
           + AGLL EV PPW + LIG++ NF G+F+IWL++T RI +P+   +C  +C+G NS +FA
Sbjct: 74  VLAGLLNEVTPPWFILLIGAILNFFGYFMIWLAVTERISKPQVWHMCLYICVGANSQSFA 133

Query: 129 NKTIMATSVVNFPDRHGIILGLLKGYVGIGGATLTQIY---------------------- 188
           N   + T V NFP+  G++LG+LKGYVG+ GA +TQ+Y                      
Sbjct: 134 NTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLYRAFYGEDTKELILMIGWLPAIV 193

Query: 189 ------------------------------------------------------------ 248
                                                                       
Sbjct: 194 SFAFLRTIRIMKVKRQTNELKVFYNFLYISLGLATFLMVVIIINKLSGFTQSEFGGSAAV 253

Query: 249 ------------------------------------------------------------ 308
                                                                       
Sbjct: 254 VIVLLLLPIIVVILEEKKLWKEKQVALNDPAPINVVTEKPKLDSSEFKDDDGEESKEVVE 313

Query: 309 ------------------------------------LATFSGSGSALAAIDNLGQVAESL 368
                                               LAT  G G  L AIDNLGQ+  SL
Sbjct: 314 KVKTPSCWTTVFNPPERGDDYTILQALFSVDMLILFLATICGVGGTLTAIDNLGQIGNSL 373

Query: 369 AYPSDAISIIISWVSVFNFFGRIFSGFISENIMMKWKLPRPLTFAVPFFITGIGQLIVAY 385
            YP  ++S  +S VS++N++GR+ SG +SE  ++K+K PRPL   +   ++  G L++A+
Sbjct: 374 GYPKRSVSTFVSLVSIWNYYGRVVSGVVSEIFLIKYKFPRPLMLTMVLLLSCAGHLLIAF 433

BLAST of Chy12G214420 vs. TAIR 10
Match: AT1G74780.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 195.3 bits (495), Expect = 9.4e-50
Identity = 137/535 (25.61%), Postives = 221/535 (41.31%), Query Frame = 0

Query: 11  QVIQGRWFSVFAGLIMMLGNGSTYIYGTYSKVIKTEFNYSQTQLSILGFAKDLGSNVGIF 70
           ++++ +W ++ A + +   +G++Y +G YS V+K+  +Y Q+ L  +   KD+G+N G+F
Sbjct: 2   EILRTKWVAMTASIWIQCASGASYTFGIYSAVLKSTQSYDQSTLDTVSVFKDIGANAGVF 61

Query: 71  AGLLAEVAP----------------PWVLFLIGSVQNFTGFFLIWLSITHRIPQPRQIFL 130
           +GLL   A                 PWV+  +G++Q F G+FLIW S+T  I +P    +
Sbjct: 62  SGLLYTYATSNRLRGRGGGIGGAGGPWVVLAVGAIQCFAGYFLIWASVTGLIRKPPVPLM 121

Query: 131 C--VCLGTNSSNFANKTIMATSVVNFPDRHGIILGLLKGYVGIGGATLTQIY-------- 190
           C  + L   S  F N   + ++V NF D  G  +G++KG++G+ GA L Q+Y        
Sbjct: 122 CLFMFLAAQSQTFFNTANVVSAVENFADYGGTAVGIMKGFLGLSGAILIQLYETLCAGDP 181

Query: 191 ------------------------------------------------------------ 250
                                                                       
Sbjct: 182 ASFILLLAVTPTVLSLLVMPLVRIYETSVADDKKHLNGLSAVSLIIAAYLMIIIILKNTF 241

Query: 251 ------------------------------------------------------------ 310
                                                                       
Sbjct: 242 GLSSWANIVTLVCLLVMLALPLLIARRAQRDGMEKTVPHDYSPLISSPKATTSGNQSSEG 301

Query: 311 ---------------------------LATFSGSGSALAAIDNLGQVAESLAYPSDAISI 370
                                      LA   G GS L+ I+N+ Q+ ESL Y S  I+ 
Sbjct: 302 DSKVEAGLSENLNLLQAMKKLSFWLLFLAMICGMGSGLSTINNIRQIGESLRYSSVEINS 361

Query: 371 IISWVSVFNFFGRIFSGFISENIMMKWKLPRPLTFAVPFFITGIGQLIVAYPSTGSVFIA 373
           ++S  S++NF GR  +G+ S+ ++ K   PRPL  A       IG LI+A    G++++ 
Sbjct: 362 LVSLWSIWNFLGRFGAGYASDALLHKKGWPRPLLMAATLGTMSIGHLIIASGFQGNLYVG 421

BLAST of Chy12G214420 vs. TAIR 10
Match: AT1G18940.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 183.0 bits (463), Expect = 4.8e-46
Identity = 126/520 (24.23%), Postives = 212/520 (40.77%), Query Frame = 0

Query: 11  QVIQGRWFSVFAGLIMMLGNGSTYIYGTYSKVIKTEFNYSQTQLSILGFAKDLGSNVGIF 70
           ++++ +W ++ A + +    G +Y +G YS ++K+  +Y Q+ L  +   KD+G NVG+ 
Sbjct: 4   EILRTKWMAMTASIWIQCSAGGSYTFGIYSAILKSTQSYDQSTLDTVSVFKDIGGNVGVL 63

Query: 71  AGLLAEVAP--------------PWVLFLIGSVQNFTGFFLIWLSITHRIPQPRQIFLC- 130
           +GL+   A               PWV+ LIG++ NFTG+FL+W S+T  I +P    +C 
Sbjct: 64  SGLVYTAATFNRRRRDGRERRGGPWVVILIGAILNFTGYFLMWASVTGLIKRPPVPVMCL 123

Query: 131 -VCLGTNSSNFANKTIMATSVVNFPDRHGIILGLLKGYVGIGGATLTQIY---------- 190
            + +   S  F N   + +S+ NF D  G  +G++KG+VG+ GA L Q+Y          
Sbjct: 124 FMFIAAQSLTFLNTANVVSSLENFADYGGTAVGIMKGFVGLSGAMLIQLYEVVCPGDPKT 183

Query: 191 ------------------------------------------------------------ 250
                                                                       
Sbjct: 184 FILLLAIVPSLLSVLVMPLVRVYKTSTVDEKKHLDGLSTLSLIIAAYLMITIILKSTLSL 243

Query: 251 ------------------------------------------------------------ 310
                                                                       
Sbjct: 244 PSWANAVTLAVLLVLLSSPLLVAVRAHRDSIEKPLSSVYSPLVDNLEATTSGEILMLDED 303

Query: 311 ------------------LATFSGSGSALAAIDNLGQVAESLAYPSDAISIIISWVSVFN 367
                             LA   G GS ++ I+N+ Q+ ESL Y S  I+ +++  +++N
Sbjct: 304 KSLNLLQAMCNVDFWLLFLAMICGMGSGISTINNIRQIGESLRYTSVEINSLLALWNIWN 363

BLAST of Chy12G214420 vs. TAIR 10
Match: AT5G50630.1 (Major facilitator superfamily protein )

HSP 1 Score: 171.0 bits (432), Expect = 1.9e-42
Identity = 127/521 (24.38%), Postives = 208/521 (39.92%), Query Frame = 0

Query: 12  VIQGRWFSVFAGLIMMLGNGSTYIY-GTYSKVIKTEFNYSQTQLSILGFAKDLGSNVGIF 71
           ++  RW      + +    G  Y++ G+ S  IKT   Y+Q Q+++LG AK+LG  +G  
Sbjct: 12  LVNDRWLVFVCAMWVQSVAGVGYLFGGSMSPAIKTSLGYNQKQIALLGVAKNLGDAIGFV 71

Query: 72  AGLLAEVAPPWVLFLIGSVQNFTGFFLIWLSITHRIPQPR--QIFLCVCLGTNSSNFANK 131
           +G L+EV+P WV+ L+G+ QN  G+ ++WL +T ++P      +F+ + +GTN   + N 
Sbjct: 72  SGALSEVSPSWVVLLVGATQNLFGYGVVWLVVTGQLPNLPLWMLFVAIFVGTNGETYYNT 131

Query: 132 TIMATSVVNFPDRHGIILGLLKGYVGIGGATLTQIY------------------------ 191
             + + + NFP+  G ++G+LKG+ G+ GA LTQ+Y                        
Sbjct: 132 ASLVSCIHNFPESRGPVVGILKGFSGLSGAILTQVYLMFNPSHDSSVILMVALGPPVVVL 191

Query: 192 ------------------------------------------------------------ 251
                                                                       
Sbjct: 192 ALLFIVRPVERSCRTNLRSDDLRFLAIYGFCVVLAVYLLGLLVLQSVFDMTQTIITTSGA 251

Query: 252 ----------LATFS--------------------------------------------- 311
                     L  FS                                             
Sbjct: 252 ILVIFMVVPVLVPFSSVFISGNNVTSVKPEEGTSNVDQHEARTLIERSDRPPEKKRAPCI 311

Query: 312 ------------------------GSGSALAAIDNLGQVAESLAYPSDAISIIISWVSVF 366
                                   G GS +  IDNLGQ+  SL Y +    I +S +S+ 
Sbjct: 312 GEDFTLLQALGQADFWLIFMSLVLGVGSGITIIDNLGQICYSLGYSN--TKIFVSLISIS 371