Chy12G214270 (gene) Cucumber (hystrix) v1

Overview


Name	Chy12G214270
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	High-affinity nitrate transporter 2.2
Location	chrH12: 13493944 .. 13495777 (+)
RNA-Seq Expression	Chy12G214270
Synteny	Chy12G214270

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTGATGTTGAAGGTTCTCCAGGAAGCTCAATGCATGGAGTGACTGGAAGAGAACAAACCTTTGCATTCTCTGTAGCTTCCCCAATCGTCCCAACTGACACCACGGCTAAATTTGCATTACCGGTCGACTCGGAGCATAAATCTAAGGTTTTCAGGATATGGTCTCTAGCCAACCCCCACATGAGAACCTTTCACCTTTCTTGGATTTCCTTCTTCACATGCTTTGTGTCCACCTTCGCAGCCGCCCCTCTCGTCCCCATCATTCGCGACAACCTGAACCTGACGAAAGTCGACATTGGGAACGCTGGAGTTACATCTGTTTCTGGAAGTATCTTCTCTCGTCTTGTGATGGGGGCTGTGTGTGACCTTCTCGGACCACGCTATGGATGTGCGTTTTTGATAATGTTGTCTGCTCCGACGGTGTTTTGTATGTCGTTTGTGTCGAATGCTGCTGGTTACATAACAGTTAGATTCATGATAGGGTTTTCGCTAGCGACGTTCGTGTCGTGTCAGTATTGGATGAGTACAATGTTTAATAGTAAGATAATAGGGCTTGTGAACGGGACAGCAGCTGGGTGGGGGAATATGGGAGGAGGAGCAACTCAATTAATAATGCCATTGTTGTACGATGTTATTCAGAGAGCTGGTGCTACTCCTTTTACTGCTTGGAGGATCGCTTTCTTCATTCCTGGTTTCCTTCATGTTCTTATGGGCATTTTGGTTTTGACTCTTGGTCAAGACTTGCCTGATGGTAACCTTGCTAGTCTTCAGAAGAAGGGAAACGTTGCCAAAGACAAATTCTCCAATGTATGTATAATTAACTTATCCTCTTAATATAATGTAATCTCTCTCATATATATGCTTTACTAATTAATTAATCATCATCTCTAATAATAAACTAAATATATATATATCCTCTTCCTCACAGGTCTTGTGGTATGCTGTGACCAATTACCGAACTTGGATCTTCGTCCTCCTCTATGGCTACTCCATGGGGGTCGAGCTTTCCACCGATAACGTCATTGCCGAATATTTCTACGATAGGTAACATAATTAAATTATATTACTATGTTTTTGTTGTGATTTAAATAGTAGGTTAGCTACATCGTCCACAAGCAAAGCGAACTAAAGAAGTACTTGTGTTGGTCGTGTATGTTAAAACAGGTTCGATCTAAAGCTTCACACAGCGGGAATCATTGCAGCAACGTTCGGGATGGCAAATCTAGTGGCTAGACCGTTCGGAGGGTATGCGTCTGACGTAGCAGCGCGATACTTCGGGATGAGAGGAAGGTTGTGGACACTATGGATCCTACAAACATGTGGGGGAGTGTTTTGCATGTGGCTGGGGAAAGCAACCAAGTTGCCAATAGCAATAGTGGCAATGATATTGTTCTCAATAGGAGCACAAGCAGCATGTGGAGCAACATTTGGTATAATTCCATTCATATCAAGGAGATCTTTGGGAATAATATCAGGACTAACAGGAGCTGGTGGGAACTTTGGAAGTGGTTTAACACAATTAGTATTCTTCTCAACAACAAAATACTCAACAGACACCGGATTGTTCTTGATGGGAATAATGATTGTTTGTTGCACTCTTCCAGTAACTTTGGTTCATTTCCCTCAATGGGGAAGCATGTTTCTACCACCAACTAAACATGTTGAAAAATCAACTGAAGAATTTTATTATGGTTCTGAATGGACTGAGGATGAAAAGAAGAAAGGTTTGCATCAACAAAGTATTAAGTTTGCTGAGAATAGCAGATCTGAACGTGGTAGACGTGTTGCTTCAGCTCCAACTCCACCAAATACAACTCCTTCTCATGTTTAA

mRNA sequence

ATGGGTGATGTTGAAGGTTCTCCAGGAAGCTCAATGCATGGAGTGACTGGAAGAGAACAAACCTTTGCATTCTCTGTAGCTTCCCCAATCGTCCCAACTGACACCACGGCTAAATTTGCATTACCGGTCGACTCGGAGCATAAATCTAAGGTTTTCAGGATATGGTCTCTAGCCAACCCCCACATGAGAACCTTTCACCTTTCTTGGATTTCCTTCTTCACATGCTTTGTGTCCACCTTCGCAGCCGCCCCTCTCGTCCCCATCATTCGCGACAACCTGAACCTGACGAAAGTCGACATTGGGAACGCTGGAGTTACATCTGTTTCTGGAAGTATCTTCTCTCGTCTTGTGATGGGGGCTGTGTGTGACCTTCTCGGACCACGCTATGGATGTGCGTTTTTGATAATGTTGTCTGCTCCGACGGTGTTTTGTATGTCGTTTGTGTCGAATGCTGCTGGTTACATAACAGTTAGATTCATGATAGGGTTTTCGCTAGCGACGTTCGTGTCGTGTCAGTATTGGATGAGTACAATGTTTAATAGTAAGATAATAGGGCTTGTGAACGGGACAGCAGCTGGGTGGGGGAATATGGGAGGAGGAGCAACTCAATTAATAATGCCATTGTTGTACGATGTTATTCAGAGAGCTGGTGCTACTCCTTTTACTGCTTGGAGGATCGCTTTCTTCATTCCTGGTTTCCTTCATGTTCTTATGGGCATTTTGGTTTTGACTCTTGGTCAAGACTTGCCTGATGGTAACCTTGCTAGTCTTCAGAAGAAGGGAAACGTTGCCAAAGACAAATTCTCCAATGTCTTGTGGTATGCTGTGACCAATTACCGAACTTGGATCTTCGTCCTCCTCTATGGCTACTCCATGGGGGTCGAGCTTTCCACCGATAACGTCATTGCCGAATATTTCTACGATAGGTTCGATCTAAAGCTTCACACAGCGGGAATCATTGCAGCAACGTTCGGGATGGCAAATCTAGTGGCTAGACCGTTCGGAGGGTATGCGTCTGACGTAGCAGCGCGATACTTCGGGATGAGAGGAAGGTTGTGGACACTATGGATCCTACAAACATGTGGGGGAGTGTTTTGCATGTGGCTGGGGAAAGCAACCAAGTTGCCAATAGCAATAGTGGCAATGATATTGTTCTCAATAGGAGCACAAGCAGCATGTGGAGCAACATTTGGTATAATTCCATTCATATCAAGGAGATCTTTGGGAATAATATCAGGACTAACAGGAGCTGGTGGGAACTTTGGAAGTGGTTTAACACAATTAGTATTCTTCTCAACAACAAAATACTCAACAGACACCGGATTGTTCTTGATGGGAATAATGATTGTTTGTTGCACTCTTCCAGTAACTTTGGTTCATTTCCCTCAATGGGGAAGCATGTTTCTACCACCAACTAAACATGTTGAAAAATCAACTGAAGAATTTTATTATGGTTCTGAATGGACTGAGGATGAAAAGAAGAAAGGTTTGCATCAACAAAGTATTAAGTTTGCTGAGAATAGCAGATCTGAACGTGGTAGACGTGTTGCTTCAGCTCCAACTCCACCAAATACAACTCCTTCTCATGTTTAA

Coding sequence (CDS)

ATGGGTGATGTTGAAGGTTCTCCAGGAAGCTCAATGCATGGAGTGACTGGAAGAGAACAAACCTTTGCATTCTCTGTAGCTTCCCCAATCGTCCCAACTGACACCACGGCTAAATTTGCATTACCGGTCGACTCGGAGCATAAATCTAAGGTTTTCAGGATATGGTCTCTAGCCAACCCCCACATGAGAACCTTTCACCTTTCTTGGATTTCCTTCTTCACATGCTTTGTGTCCACCTTCGCAGCCGCCCCTCTCGTCCCCATCATTCGCGACAACCTGAACCTGACGAAAGTCGACATTGGGAACGCTGGAGTTACATCTGTTTCTGGAAGTATCTTCTCTCGTCTTGTGATGGGGGCTGTGTGTGACCTTCTCGGACCACGCTATGGATGTGCGTTTTTGATAATGTTGTCTGCTCCGACGGTGTTTTGTATGTCGTTTGTGTCGAATGCTGCTGGTTACATAACAGTTAGATTCATGATAGGGTTTTCGCTAGCGACGTTCGTGTCGTGTCAGTATTGGATGAGTACAATGTTTAATAGTAAGATAATAGGGCTTGTGAACGGGACAGCAGCTGGGTGGGGGAATATGGGAGGAGGAGCAACTCAATTAATAATGCCATTGTTGTACGATGTTATTCAGAGAGCTGGTGCTACTCCTTTTACTGCTTGGAGGATCGCTTTCTTCATTCCTGGTTTCCTTCATGTTCTTATGGGCATTTTGGTTTTGACTCTTGGTCAAGACTTGCCTGATGGTAACCTTGCTAGTCTTCAGAAGAAGGGAAACGTTGCCAAAGACAAATTCTCCAATGTCTTGTGGTATGCTGTGACCAATTACCGAACTTGGATCTTCGTCCTCCTCTATGGCTACTCCATGGGGGTCGAGCTTTCCACCGATAACGTCATTGCCGAATATTTCTACGATAGGTTCGATCTAAAGCTTCACACAGCGGGAATCATTGCAGCAACGTTCGGGATGGCAAATCTAGTGGCTAGACCGTTCGGAGGGTATGCGTCTGACGTAGCAGCGCGATACTTCGGGATGAGAGGAAGGTTGTGGACACTATGGATCCTACAAACATGTGGGGGAGTGTTTTGCATGTGGCTGGGGAAAGCAACCAAGTTGCCAATAGCAATAGTGGCAATGATATTGTTCTCAATAGGAGCACAAGCAGCATGTGGAGCAACATTTGGTATAATTCCATTCATATCAAGGAGATCTTTGGGAATAATATCAGGACTAACAGGAGCTGGTGGGAACTTTGGAAGTGGTTTAACACAATTAGTATTCTTCTCAACAACAAAATACTCAACAGACACCGGATTGTTCTTGATGGGAATAATGATTGTTTGTTGCACTCTTCCAGTAACTTTGGTTCATTTCCCTCAATGGGGAAGCATGTTTCTACCACCAACTAAACATGTTGAAAAATCAACTGAAGAATTTTATTATGGTTCTGAATGGACTGAGGATGAAAAGAAGAAAGGTTTGCATCAACAAAGTATTAAGTTTGCTGAGAATAGCAGATCTGAACGTGGTAGACGTGTTGCTTCAGCTCCAACTCCACCAAATACAACTCCTTCTCATGTTTAA

Protein sequence

MGDVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKSKVFRIWSLANPHMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAVCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYITVRFMIGFSLATFVSCQYWMSTMFNSKIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGFLHVLMGILVLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNVIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTCGGVFCMWLGKATKLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNFGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEEFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSHV*

Homology

[Close]

BLAST of Chy12G214270 vs. ExPASy Swiss-Prot
Match: Q9FJH8 (High affinity nitrate transporter 2.4 OS=Arabidopsis thaliana OX=3702 GN=NRT2.4 PE=2 SV=1)

HSP 1 Score: 863.6 bits (2230), Expect = 1.2e-249
Identity = 420/530 (79.25%), Postives = 476/530 (89.81%), Query Frame = 0


Query: 1   MGDVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKSKVFRIWSLANP 60
           M D  G PGSSMHGVTGREQ++AFSV SP VP+D++AKF+LPVD+EHK+KVF++ S   P
Sbjct: 1   MADGFGEPGSSMHGVTGREQSYAFSVESPAVPSDSSAKFSLPVDTEHKAKVFKLLSFEAP 60

Query: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120
           HMRTFHL+WISFFTCF+STFAAAPLVPIIRDNLNLT+ D+GNAGV SVSGSIFSRLVMGA
Sbjct: 61  HMRTFHLAWISFFTCFISTFAAAPLVPIIRDNLNLTRQDVGNAGVASVSGSIFSRLVMGA 120

Query: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYITVRFMIGFSLATFVSCQYWMSTMFN 180
           VCDLLGPRYGCAFL+MLSAPTVF MSFV  A GYITVRFMIGF LATFVSCQYWMSTMFN
Sbjct: 121 VCDLLGPRYGCAFLVMLSAPTVFSMSFVGGAGGYITVRFMIGFCLATFVSCQYWMSTMFN 180

Query: 181 SKIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGFLHVLMGI 240
            +IIGLVNGTAAGWGNMGGG TQL+MP++Y++I+R G+T FTAWR+AFF+PG++H++MGI
Sbjct: 181 GQIIGLVNGTAAGWGNMGGGVTQLLMPMVYEIIRRLGSTSFTAWRMAFFVPGWMHIIMGI 240

Query: 241 LVLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDN 300
           LVLTLGQDLPDGN ++L+KKG V KDKFS VLWYA+TNYRTW+FVLLYGYSMGVEL+TDN
Sbjct: 241 LVLTLGQDLPDGNRSTLEKKGAVTKDKFSKVLWYAITNYRTWVFVLLYGYSMGVELTTDN 300

Query: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT 360
           VIAEYF+DRF LKLHTAGIIAA+FGMAN  ARP GG+ASD+AAR FGMRGRLWTLWI+QT
Sbjct: 301 VIAEYFFDRFHLKLHTAGIIAASFGMANFFARPIGGWASDIAARRFGMRGRLWTLWIIQT 360

Query: 361 CGGVFCMWLGKATKLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420
            GG FC+WLG+AT LP A+V MILFS+GAQAACGATF IIPFISRRSLGIISGLTGAGGN
Sbjct: 361 LGGFFCLWLGRATTLPTAVVFMILFSLGAQAACGATFAIIPFISRRSLGIISGLTGAGGN 420

Query: 421 FGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE 480
           FGSGLTQLVFFST+ +ST+ GL  MG+MI+ CTLPVTLVHFPQWGSMFLP T+   KSTE
Sbjct: 421 FGSGLTQLVFFSTSTFSTEQGLTWMGVMIMACTLPVTLVHFPQWGSMFLPSTEDEVKSTE 480

Query: 481 EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSHV 531
           E+YY  EWTE EK+KG+H+ S+KFA NSRSERGRRVASAP+PP   P HV
Sbjct: 481 EYYYMKEWTETEKRKGMHEGSLKFAVNSRSERGRRVASAPSPP---PEHV 527

[Close]

BLAST of Chy12G214270 vs. ExPASy Swiss-Prot
Match: O82811 (High-affinity nitrate transporter 2.1 OS=Arabidopsis thaliana OX=3702 GN=NRT2.1 PE=1 SV=1)

HSP 1 Score: 862.1 bits (2226), Expect = 3.4e-249
Identity = 420/530 (79.25%), Postives = 473/530 (89.25%), Query Frame = 0


Query: 1   MGDVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKSKVFRIWSLANP 60
           MGD  G PGSSMHGVTGREQ+FAFSV SPIV TD TAKF LPVD+EHK+ VF+++S A P
Sbjct: 1   MGDSTGEPGSSMHGVTGREQSFAFSVQSPIVHTDKTAKFDLPVDTEHKATVFKLFSFAKP 60

Query: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120
           HMRTFHLSWISF TCFVSTFAAAPLVPIIR+NLNLTK DIGNAGV SVSGSIFSRLVMGA
Sbjct: 61  HMRTFHLSWISFSTCFVSTFAAAPLVPIIRENLNLTKQDIGNAGVASVSGSIFSRLVMGA 120

Query: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYITVRFMIGFSLATFVSCQYWMSTMFN 180
           VCDLLGPRYGCAFL+MLSAPTVF MSFVS+AAG+ITVRFMIGF LATFVSCQYWMSTMFN
Sbjct: 121 VCDLLGPRYGCAFLVMLSAPTVFSMSFVSDAAGFITVRFMIGFCLATFVSCQYWMSTMFN 180

Query: 181 SKIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGFLHVLMGI 240
           S+IIGLVNGTAAGWGNMGGG TQL+MP++Y++I+R G+T FTAWRIAFF+PG+LH++MGI
Sbjct: 181 SQIIGLVNGTAAGWGNMGGGITQLLMPIVYEIIRRCGSTAFTAWRIAFFVPGWLHIIMGI 240

Query: 241 LVLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDN 300
           LVL LGQDLPDGN A+L+K G VAKDKF  +LWYAVTNYRTWIFVLLYGYSMGVELSTDN
Sbjct: 241 LVLNLGQDLPDGNRATLEKAGEVAKDKFGKILWYAVTNYRTWIFVLLYGYSMGVELSTDN 300

Query: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT 360
           VIAEYF+DRF LKLHTAG+IAA FGMAN  ARP GGYASD AA+YFGMRGRLWTLWI+QT
Sbjct: 301 VIAEYFFDRFHLKLHTAGLIAACFGMANFFARPAGGYASDFAAKYFGMRGRLWTLWIIQT 360

Query: 361 CGGVFCMWLGKATKLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420
            GG+FC+WLG+A  L  A+VAM+LFS+GAQAACGATF I+PF+SRR+LGIISGLTGAGGN
Sbjct: 361 AGGLFCVWLGRANTLVTAVVAMVLFSMGAQAACGATFAIVPFVSRRALGIISGLTGAGGN 420

Query: 421 FGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE 480
           FGSGLTQL+FFST+ ++T+ GL  MG+MIV CTLPVTLVHFPQWGSMFLPP+    K TE
Sbjct: 421 FGSGLTQLLFFSTSHFTTEQGLTWMGVMIVACTLPVTLVHFPQWGSMFLPPSTDPVKGTE 480

Query: 481 EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSHV 531
             YYGSEW E EK+K +HQ S++FAEN++SE GRRV SA TPP  TP++V
Sbjct: 481 AHYYGSEWNEQEKQKNMHQGSLRFAENAKSEGGRRVRSAATPPENTPNNV 530

[Close]

BLAST of Chy12G214270 vs. ExPASy Swiss-Prot
Match: P0DKG9 (High-affinity nitrate transporter 2.1 OS=Oryza sativa subsp. japonica OX=39947 GN=NRT2.1 PE=1 SV=1)

HSP 1 Score: 822.4 bits (2123), Expect = 3.0e-237
Identity = 407/529 (76.94%), Postives = 454/529 (85.82%), Query Frame = 0


Query: 6   GSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKSKVFRIWSLANPHMRTF 65
           G+PGSS+HGVTGRE  FAFS           +KF LPVDSEHK+K  R+ S ANPHMRTF
Sbjct: 7   GAPGSSLHGVTGREPAFAFSTEVGGEDAAAASKFDLPVDSEHKAKTIRLLSFANPHMRTF 66

Query: 66  HLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAVCDLL 125
           HLSWISFF+CFVSTFAAAPLVPIIRDNLNLTK DIGNAGV SVSGSIFSRL MGA+CD+L
Sbjct: 67  HLSWISFFSCFVSTFAAAPLVPIIRDNLNLTKADIGNAGVASVSGSIFSRLAMGAICDML 126

Query: 126 GPRYGCAFLIMLSAPTVFCMSFVSNAAGYITVRFMIGFSLATFVSCQYWMSTMFNSKIIG 185
           GPRYGCAFLIML+APTVFCMS + +AAGYI VRF+IGFSLATFVSCQYWMSTMFNSKIIG
Sbjct: 127 GPRYGCAFLIMLAAPTVFCMSLIDSAAGYIAVRFLIGFSLATFVSCQYWMSTMFNSKIIG 186

Query: 186 LVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGFLHVLMGILVLTL 245
           LVNG AAGWGNMGGGATQLIMPL+YDVI++ GATPFTAWR+A+F+PG LHV+MG+LVLTL
Sbjct: 187 LVNGLAAGWGNMGGGATQLIMPLVYDVIRKCGATPFTAWRLAYFVPGTLHVVMGVLVLTL 246

Query: 246 GQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNVIAEY 305
           GQDLPDGNL SLQKKG+V +D FS VLWYAVTNYRTWIFVLLYGYSMGVEL+TDNVIAEY
Sbjct: 247 GQDLPDGNLRSLQKKGDVNRDSFSRVLWYAVTNYRTWIFVLLYGYSMGVELTTDNVIAEY 306

Query: 306 FYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTCGGVF 365
           FYDRFDL L  AGIIAA+FGMAN+VARP GG  SD+ ARYFGMR RLW +WILQT GG F
Sbjct: 307 FYDRFDLDLRVAGIIAASFGMANIVARPTGGLLSDLGARYFGMRARLWNIWILQTAGGAF 366

Query: 366 CMWLGKATKLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNFGSGL 425
           C+ LG+A+ LP ++V M+LFS  AQAACGA FG+IPF+SRRSLGIISG+TGAGGNFG+GL
Sbjct: 367 CLLLGRASTLPTSVVCMVLFSFCAQAACGAIFGVIPFVSRRSLGIISGMTGAGGNFGAGL 426

Query: 426 TQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEEFYYG 485
           TQL+FF++++YST TGL  MGIMI+ CTLPV LVHFPQWGSMFLPP    E   EE YYG
Sbjct: 427 TQLLFFTSSRYSTGTGLEYMGIMIMACTLPVVLVHFPQWGSMFLPPNAGAE---EEHYYG 486

Query: 486 SEWTEDEKKKGLHQQSIKFAENSRSERGRR-----VASAPTPPNTTPSH 530
           SEW+E EK KGLH  S+KFAENSRSERGRR      A+A TPPN +P H
Sbjct: 487 SEWSEQEKSKGLHGASLKFAENSRSERGRRNVINAAAAAATPPNNSPEH 532

[Close]

BLAST of Chy12G214270 vs. ExPASy Swiss-Prot
Match: P0DKH0 (High-affinity nitrate transporter 2.2 OS=Oryza sativa subsp. japonica OX=39947 GN=NRT2.2 PE=1 SV=1)

HSP 1 Score: 822.4 bits (2123), Expect = 3.0e-237
Identity = 407/529 (76.94%), Postives = 454/529 (85.82%), Query Frame = 0


Query: 6   GSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKSKVFRIWSLANPHMRTF 65
           G+PGSS+HGVTGRE  FAFS           +KF LPVDSEHK+K  R+ S ANPHMRTF
Sbjct: 7   GAPGSSLHGVTGREPAFAFSTEVGGEDAAAASKFDLPVDSEHKAKTIRLLSFANPHMRTF 66

Query: 66  HLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAVCDLL 125
           HLSWISFF+CFVSTFAAAPLVPIIRDNLNLTK DIGNAGV SVSGSIFSRL MGA+CD+L
Sbjct: 67  HLSWISFFSCFVSTFAAAPLVPIIRDNLNLTKADIGNAGVASVSGSIFSRLAMGAICDML 126

Query: 126 GPRYGCAFLIMLSAPTVFCMSFVSNAAGYITVRFMIGFSLATFVSCQYWMSTMFNSKIIG 185
           GPRYGCAFLIML+APTVFCMS + +AAGYI VRF+IGFSLATFVSCQYWMSTMFNSKIIG
Sbjct: 127 GPRYGCAFLIMLAAPTVFCMSLIDSAAGYIAVRFLIGFSLATFVSCQYWMSTMFNSKIIG 186

Query: 186 LVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGFLHVLMGILVLTL 245
           LVNG AAGWGNMGGGATQLIMPL+YDVI++ GATPFTAWR+A+F+PG LHV+MG+LVLTL
Sbjct: 187 LVNGLAAGWGNMGGGATQLIMPLVYDVIRKCGATPFTAWRLAYFVPGTLHVVMGVLVLTL 246

Query: 246 GQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNVIAEY 305
           GQDLPDGNL SLQKKG+V +D FS VLWYAVTNYRTWIFVLLYGYSMGVEL+TDNVIAEY
Sbjct: 247 GQDLPDGNLRSLQKKGDVNRDSFSRVLWYAVTNYRTWIFVLLYGYSMGVELTTDNVIAEY 306

Query: 306 FYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTCGGVF 365
           FYDRFDL L  AGIIAA+FGMAN+VARP GG  SD+ ARYFGMR RLW +WILQT GG F
Sbjct: 307 FYDRFDLDLRVAGIIAASFGMANIVARPTGGLLSDLGARYFGMRARLWNIWILQTAGGAF 366

Query: 366 CMWLGKATKLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNFGSGL 425
           C+ LG+A+ LP ++V M+LFS  AQAACGA FG+IPF+SRRSLGIISG+TGAGGNFG+GL
Sbjct: 367 CLLLGRASTLPTSVVCMVLFSFCAQAACGAIFGVIPFVSRRSLGIISGMTGAGGNFGAGL 426

Query: 426 TQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEEFYYG 485
           TQL+FF++++YST TGL  MGIMI+ CTLPV LVHFPQWGSMFLPP    E   EE YYG
Sbjct: 427 TQLLFFTSSRYSTGTGLEYMGIMIMACTLPVVLVHFPQWGSMFLPPNAGAE---EEHYYG 486

Query: 486 SEWTEDEKKKGLHQQSIKFAENSRSERGRR-----VASAPTPPNTTPSH 530
           SEW+E EK KGLH  S+KFAENSRSERGRR      A+A TPPN +P H
Sbjct: 487 SEWSEQEKSKGLHGASLKFAENSRSERGRRNVINAAAAAATPPNNSPEH 532

[Close]

BLAST of Chy12G214270 vs. ExPASy Swiss-Prot
Match: Q9FJH7 (High affinity nitrate transporter 2.3 OS=Arabidopsis thaliana OX=3702 GN=NRT2.3 PE=1 SV=1)

HSP 1 Score: 791.6 bits (2043), Expect = 5.6e-228
Identity = 391/531 (73.63%), Postives = 451/531 (84.93%), Query Frame = 0


Query: 5   EGSPGSSMHGVTGREQTFAFSV---ASPIVPTDTTAKFALPVDSEHKSKVFRIWSLANPH 64
           EGS G+S+HGVT REQ F+FSV   +  +   D TAKFALPVDSEH++KVF   S A PH
Sbjct: 9   EGSIGTSLHGVTAREQVFSFSVDASSQTVQSDDPTAKFALPVDSEHRAKVFNPLSFAKPH 68

Query: 65  MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAV 124
           MR FHL W+SFFTCF+STFAAAPLVPIIRDNL+LTK DIGNAGV SVSG+IFSRL MGAV
Sbjct: 69  MRAFHLGWLSFFTCFISTFAAAPLVPIIRDNLDLTKTDIGNAGVASVSGAIFSRLAMGAV 128

Query: 125 CDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYITVRFMIGFSLATFVSCQYWMSTMFNS 184
           CDLLG RYG AF +ML+APTVF MSFV   +GY+ VRFMIGF LATFVSCQYW S MFN 
Sbjct: 129 CDLLGARYGTAFSLMLTAPTVFSMSFVGGPSGYLGVRFMIGFCLATFVSCQYWTSVMFNG 188

Query: 185 KIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGFLHVLMGIL 244
           KIIGLVNG A GWG+MGGG TQL+MP+++ VI+ AGATPF AWRIAFF+PGFL V+MGIL
Sbjct: 189 KIIGLVNGCAGGWGDMGGGVTQLLMPMVFHVIKLAGATPFMAWRIAFFVPGFLQVVMGIL 248

Query: 245 VLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV 304
           VL+LGQDLPDGNL++LQK G V+KDKFS V W+AV NYRTWI  +LYG SMG+EL+ +NV
Sbjct: 249 VLSLGQDLPDGNLSTLQKSGQVSKDKFSKVFWFAVKNYRTWILFVLYGSSMGIELTINNV 308

Query: 305 IAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTC 364
           I+ YFYDRF+LKL TAGI+AA+FGMAN +ARPFGGYASDVAAR FGMRGRLWTLWI QT 
Sbjct: 309 ISGYFYDRFNLKLQTAGIVAASFGMANFIARPFGGYASDVAARVFGMRGRLWTLWIFQTV 368

Query: 365 GGVFCMWLGKATKLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 424
           G +FC+WLG+A+ LPIAI+AM+LFSIG QAACGA FG+ PF+SRRSLG+ISGLTGAGGNF
Sbjct: 369 GALFCIWLGRASSLPIAILAMMLFSIGTQAACGALFGVAPFVSRRSLGLISGLTGAGGNF 428

Query: 425 GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEE 484
           GSGLTQL+FFS+ ++ST  GL LMG+M V CTLPV  +HFPQWGSMFL P+   E+S EE
Sbjct: 429 GSGLTQLLFFSSARFSTAEGLSLMGVMAVLCTLPVAFIHFPQWGSMFLRPSTDGERSQEE 488

Query: 485 FYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASA--PTPPNTTPSHV 531
           +YYGSEWTE+EK++GLH+ SIKFAENSRSERGR+VA A  PTP N TPSHV
Sbjct: 489 YYYGSEWTENEKQQGLHEGSIKFAENSRSERGRKVALANIPTPENGTPSHV 539

[Close]

BLAST of Chy12G214270 vs. ExPASy TrEMBL
Match: A0A3Q9CZ42 (High affinity nitrate transporter 2.1 OS=Cucumis sativus OX=3659 GN=NRT2.1 PE=2 SV=1)

HSP 1 Score: 1045.8 bits (2703), Expect = 6.1e-302
Identity = 523/530 (98.68%), Postives = 527/530 (99.43%), Query Frame = 0


Query: 1   MGDVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKSKVFRIWSLANP 60
           MGDVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHK+KVFRIWSLANP
Sbjct: 1   MGDVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFRIWSLANP 60

Query: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120
           HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA
Sbjct: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120

Query: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYITVRFMIGFSLATFVSCQYWMSTMFN 180
           VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYI VRFMIGFSLATFVSCQYWMSTMFN
Sbjct: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180

Query: 181 SKIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGFLHVLMGI 240
           S+IIGLVNGTAAGWGNMGGGATQLIMPLLY+VIQRAGATPFTAWRIAFFIPGFLHVLMGI
Sbjct: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYEVIQRAGATPFTAWRIAFFIPGFLHVLMGI 240

Query: 241 LVLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDN 300
           LVLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDN
Sbjct: 241 LVLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDN 300

Query: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT 360
           VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT
Sbjct: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT 360

Query: 361 CGGVFCMWLGKATKLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420
            GGVFCMWLGKATKLPIAI AMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN
Sbjct: 361 FGGVFCMWLGKATKLPIAIAAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420

Query: 421 FGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE 480
           FGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE
Sbjct: 421 FGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE 480

Query: 481 EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSHV 531
           EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTP+HV
Sbjct: 481 EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPTHV 530

[Close]

BLAST of Chy12G214270 vs. ExPASy TrEMBL
Match: Q6PRP8 (High-affinity nitrate transporter OS=Cucumis sativus OX=3659 GN=NRT2 PE=2 SV=3)

HSP 1 Score: 1043.9 bits (2698), Expect = 2.3e-301
Identity = 523/530 (98.68%), Postives = 526/530 (99.25%), Query Frame = 0


Query: 1   MGDVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKSKVFRIWSLANP 60
           MGDVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHK+KVFRIWSLANP
Sbjct: 1   MGDVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFRIWSLANP 60

Query: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120
           HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA
Sbjct: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120

Query: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYITVRFMIGFSLATFVSCQYWMSTMFN 180
           VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYI VRFMIGFSLATFVSCQYWMSTMFN
Sbjct: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180

Query: 181 SKIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGFLHVLMGI 240
           S+IIGLVNGTAAGWGNMGGGATQLIMPLLY+VIQRAGATPFTAWRIAFFIPGFLHVLMGI
Sbjct: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYEVIQRAGATPFTAWRIAFFIPGFLHVLMGI 240

Query: 241 LVLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDN 300
           LVLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDN
Sbjct: 241 LVLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDN 300

Query: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT 360
           VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT
Sbjct: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT 360

Query: 361 CGGVFCMWLGKATKLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420
            GGVFCMWLGKATKLPIAI AMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN
Sbjct: 361 FGGVFCMWLGKATKLPIAIAAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420

Query: 421 FGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE 480
           FGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE
Sbjct: 421 FGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE 480

Query: 481 EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSHV 531
           EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPS V
Sbjct: 481 EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSRV 530

[Close]

BLAST of Chy12G214270 vs. ExPASy TrEMBL
Match: S4VM42 (High-affinity nitrate transporter NRT2.2 OS=Cucumis sativus OX=3659 GN=NRT2.2 PE=2 SV=1)

HSP 1 Score: 1043.5 bits (2697), Expect = 3.0e-301
Identity = 521/530 (98.30%), Postives = 527/530 (99.43%), Query Frame = 0


Query: 1   MGDVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKSKVFRIWSLANP 60
           MGDVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHK+KVFRIWSLANP
Sbjct: 1   MGDVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFRIWSLANP 60

Query: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120
           HMRTFHLSW+SFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA
Sbjct: 61  HMRTFHLSWVSFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120

Query: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYITVRFMIGFSLATFVSCQYWMSTMFN 180
           VCDLLGPRYGCAFLIMLSAPTVFCMSFVS+AAGYI VRFMIGFSLATFVSCQYWMSTMFN
Sbjct: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSDAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180

Query: 181 SKIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGFLHVLMGI 240
           S+IIGLVNGTAAGWGNMGGGATQLIMPLLY+VIQRAGATPFTAWRIAFFIPGFLHVLMGI
Sbjct: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYEVIQRAGATPFTAWRIAFFIPGFLHVLMGI 240

Query: 241 LVLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDN 300
           LVLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDN
Sbjct: 241 LVLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDN 300

Query: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT 360
           VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT
Sbjct: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT 360

Query: 361 CGGVFCMWLGKATKLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420
            GGVFCMWLGKATKLPIAI AMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN
Sbjct: 361 FGGVFCMWLGKATKLPIAIAAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420

Query: 421 FGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE 480
           FGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE
Sbjct: 421 FGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE 480

Query: 481 EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSHV 531
           EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTP+HV
Sbjct: 481 EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPTHV 530

[Close]

BLAST of Chy12G214270 vs. ExPASy TrEMBL
Match: A0A6J1DHB7 (high affinity nitrate transporter 2.4-like OS=Momordica charantia OX=3673 GN=LOC111020453 PE=4 SV=1)

HSP 1 Score: 1013.1 bits (2618), Expect = 4.4e-292
Identity = 501/529 (94.71%), Postives = 520/529 (98.30%), Query Frame = 0


Query: 2   GDVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKSKVFRIWSLANPH 61
           G+VEGSPGSSMHGVTGREQTFAFSVASPIVPTDT+AKFALPVDSEHK+KVF+I+SLANPH
Sbjct: 6   GNVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTSAKFALPVDSEHKAKVFKIFSLANPH 65

Query: 62  MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAV 121
           MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTK DIGNAGVTSVSGSIFSRLVMGAV
Sbjct: 66  MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKSDIGNAGVTSVSGSIFSRLVMGAV 125

Query: 122 CDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYITVRFMIGFSLATFVSCQYWMSTMFNS 181
           CDLLGPRYGCAFLIMLSAPTVFCMSFVS+ AGYI VRFMIGFSLATFVSCQYWMSTMFNS
Sbjct: 126 CDLLGPRYGCAFLIMLSAPTVFCMSFVSSPAGYIAVRFMIGFSLATFVSCQYWMSTMFNS 185

Query: 182 KIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGFLHVLMGIL 241
           +IIGLVNGTAAGWGNMGGGATQL+MPLLY++I++ GATPFTAWRIAFFIPG LHVLMGIL
Sbjct: 186 QIIGLVNGTAAGWGNMGGGATQLLMPLLYEIIKKVGATPFTAWRIAFFIPGSLHVLMGIL 245

Query: 242 VLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV 301
           V+TLGQDLPDGNL+SLQKKG VAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV
Sbjct: 246 VMTLGQDLPDGNLSSLQKKGTVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV 305

Query: 302 IAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTC 361
           +AEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASD AARYFGMRGRLWTLWILQTC
Sbjct: 306 VAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQTC 365

Query: 362 GGVFCMWLGKATKLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 421
           GGVFCMWLGKAT+LP+AIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF
Sbjct: 366 GGVFCMWLGKATQLPVAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 425

Query: 422 GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEE 481
           GSGLTQLVFFSTTKYSTD GLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEE
Sbjct: 426 GSGLTQLVFFSTTKYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEE 485

Query: 482 FYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSHV 531
           FYYGSEWTEDEKKKGLHQQSIKFAENSRSERGR++ASAPTPPN TPSHV
Sbjct: 486 FYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRKIASAPTPPNATPSHV 534

[Close]

BLAST of Chy12G214270 vs. ExPASy TrEMBL
Match: A0A6J1HIH1 (high affinity nitrate transporter 2.4-like OS=Cucurbita moschata OX=3662 GN=LOC111464708 PE=4 SV=1)

HSP 1 Score: 1012.7 bits (2617), Expect = 5.7e-292
Identity = 504/530 (95.09%), Postives = 518/530 (97.74%), Query Frame = 0


Query: 1   MGDVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKSKVFRIWSLANP 60
           M  VEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHK+KVF+++S ANP
Sbjct: 1   MSGVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKLFSFANP 60

Query: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120
           HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA
Sbjct: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120

Query: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYITVRFMIGFSLATFVSCQYWMSTMFN 180
           VCDLLGPRYGCAFLIMLSAPTVFCMSFVS+AAGYI VRFMIGFSLATFVSCQYWMSTMFN
Sbjct: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSDAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180

Query: 181 SKIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGFLHVLMGI 240
           S+IIGLVNGTAAGWGNMGGGATQLIMPLLYD+I+RAGATPFTAWRIAFFIPG+LHV+MGI
Sbjct: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYDIIKRAGATPFTAWRIAFFIPGWLHVIMGI 240

Query: 241 LVLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDN 300
           LVLTLGQDLPDGNL+SLQKKG+VAKDKFS VLWYA+TNYRTWIFVLLYGYSMGVELSTDN
Sbjct: 241 LVLTLGQDLPDGNLSSLQKKGDVAKDKFSKVLWYAITNYRTWIFVLLYGYSMGVELSTDN 300

Query: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT 360
           VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASD AARYFGMRGRLWTLWILQT
Sbjct: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQT 360

Query: 361 CGGVFCMWLGKATKLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420
            GGVFCMWLGKA KLP AI AMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN
Sbjct: 361 FGGVFCMWLGKADKLPTAIAAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420

Query: 421 FGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE 480
           FGSGLTQLVFFSTTKYSTD GLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE
Sbjct: 421 FGSGLTQLVFFSTTKYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE 480

Query: 481 EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSHV 531
           EFYYG+EWTEDEKKKGLHQQSIKFAENSRSERGRRV SAPTPPNTTPSHV
Sbjct: 481 EFYYGAEWTEDEKKKGLHQQSIKFAENSRSERGRRVGSAPTPPNTTPSHV 530

[Close]

BLAST of Chy12G214270 vs. NCBI nr
Match: XP_031736393.1 (high affinity nitrate transporter 2.4-like isoform X1 [Cucumis sativus] >AZP53714.1 high affinity nitrate transporter 2.1 [Cucumis sativus] >KAE8652752.1 hypothetical protein Csa_014056 [Cucumis sativus])

HSP 1 Score: 1045 bits (2703), Expect = 0.0
Identity = 523/530 (98.68%), Postives = 527/530 (99.43%), Query Frame = 0


Query: 1   MGDVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKSKVFRIWSLANP 60
           MGDVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHK+KVFRIWSLANP
Sbjct: 1   MGDVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFRIWSLANP 60

Query: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120
           HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA
Sbjct: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120

Query: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYITVRFMIGFSLATFVSCQYWMSTMFN 180
           VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYI VRFMIGFSLATFVSCQYWMSTMFN
Sbjct: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180

Query: 181 SKIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGFLHVLMGI 240
           S+IIGLVNGTAAGWGNMGGGATQLIMPLLY+VIQRAGATPFTAWRIAFFIPGFLHVLMGI
Sbjct: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYEVIQRAGATPFTAWRIAFFIPGFLHVLMGI 240

Query: 241 LVLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDN 300
           LVLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDN
Sbjct: 241 LVLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDN 300

Query: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT 360
           VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT
Sbjct: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT 360

Query: 361 CGGVFCMWLGKATKLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420
            GGVFCMWLGKATKLPIAI AMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN
Sbjct: 361 FGGVFCMWLGKATKLPIAIAAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420

Query: 421 FGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE 480
           FGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE
Sbjct: 421 FGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE 480

Query: 481 EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSHV 530
           EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTP+HV
Sbjct: 481 EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPTHV 530

[Close]

BLAST of Chy12G214270 vs. NCBI nr
Match: NP_001274401.1 (high affinity nitrate transporter 2.4-like [Cucumis sativus] >AAS93686.3 high-affinity nitrate transporter [Cucumis sativus])

HSP 1 Score: 1043 bits (2698), Expect = 0.0
Identity = 523/530 (98.68%), Postives = 526/530 (99.25%), Query Frame = 0


Query: 1   MGDVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKSKVFRIWSLANP 60
           MGDVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHK+KVFRIWSLANP
Sbjct: 1   MGDVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFRIWSLANP 60

Query: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120
           HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA
Sbjct: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120

Query: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYITVRFMIGFSLATFVSCQYWMSTMFN 180
           VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYI VRFMIGFSLATFVSCQYWMSTMFN
Sbjct: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180

Query: 181 SKIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGFLHVLMGI 240
           S+IIGLVNGTAAGWGNMGGGATQLIMPLLY+VIQRAGATPFTAWRIAFFIPGFLHVLMGI
Sbjct: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYEVIQRAGATPFTAWRIAFFIPGFLHVLMGI 240

Query: 241 LVLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDN 300
           LVLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDN
Sbjct: 241 LVLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDN 300

Query: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT 360
           VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT
Sbjct: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT 360

Query: 361 CGGVFCMWLGKATKLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420
            GGVFCMWLGKATKLPIAI AMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN
Sbjct: 361 FGGVFCMWLGKATKLPIAIAAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420

Query: 421 FGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE 480
           FGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE
Sbjct: 421 FGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE 480

Query: 481 EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSHV 530
           EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPS V
Sbjct: 481 EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSRV 530

[Close]

BLAST of Chy12G214270 vs. NCBI nr
Match: AGO64298.1 (high-affinity nitrate transporter NRT2.2 [Cucumis sativus])

HSP 1 Score: 1043 bits (2697), Expect = 0.0
Identity = 521/530 (98.30%), Postives = 527/530 (99.43%), Query Frame = 0


Query: 1   MGDVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKSKVFRIWSLANP 60
           MGDVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHK+KVFRIWSLANP
Sbjct: 1   MGDVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFRIWSLANP 60

Query: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120
           HMRTFHLSW+SFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA
Sbjct: 61  HMRTFHLSWVSFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120

Query: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYITVRFMIGFSLATFVSCQYWMSTMFN 180
           VCDLLGPRYGCAFLIMLSAPTVFCMSFVS+AAGYI VRFMIGFSLATFVSCQYWMSTMFN
Sbjct: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSDAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180

Query: 181 SKIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGFLHVLMGI 240
           S+IIGLVNGTAAGWGNMGGGATQLIMPLLY+VIQRAGATPFTAWRIAFFIPGFLHVLMGI
Sbjct: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYEVIQRAGATPFTAWRIAFFIPGFLHVLMGI 240

Query: 241 LVLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDN 300
           LVLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDN
Sbjct: 241 LVLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDN 300

Query: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT 360
           VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT
Sbjct: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT 360

Query: 361 CGGVFCMWLGKATKLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420
            GGVFCMWLGKATKLPIAI AMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN
Sbjct: 361 FGGVFCMWLGKATKLPIAIAAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420

Query: 421 FGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE 480
           FGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE
Sbjct: 421 FGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE 480

Query: 481 EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSHV 530
           EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTP+HV
Sbjct: 481 EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPTHV 530

[Close]

BLAST of Chy12G214270 vs. NCBI nr
Match: NP_001269063.2 (high affinity nitrate transporter 2.4-like [Cucumis sativus])

HSP 1 Score: 1039 bits (2687), Expect = 0.0
Identity = 520/530 (98.11%), Postives = 525/530 (99.06%), Query Frame = 0


Query: 1   MGDVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKSKVFRIWSLANP 60
           MGDVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHK+KVFRIWSLANP
Sbjct: 1   MGDVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFRIWSLANP 60

Query: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120
           HMRTFHLSW+SFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA
Sbjct: 61  HMRTFHLSWVSFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120

Query: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYITVRFMIGFSLATFVSCQYWMSTMFN 180
           VCDLLGPRYGCAFLIMLSAPTVFCMSFVS+AAGYI VRFMIGFSLATFVSCQYWMSTMFN
Sbjct: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSDAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180

Query: 181 SKIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGFLHVLMGI 240
           S+IIGLVNGTAAGWGNMGGGATQLIMPLLY+VIQRAGATPFTAWRIAFFIPGFLHVLMGI
Sbjct: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYEVIQRAGATPFTAWRIAFFIPGFLHVLMGI 240

Query: 241 LVLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDN 300
           LVLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDN
Sbjct: 241 LVLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDN 300

Query: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT 360
           VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLW LWILQT
Sbjct: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWALWILQT 360

Query: 361 CGGVFCMWLGKATKLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420
            GGVFCMWLGKATKLPIAI AMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN
Sbjct: 361 FGGVFCMWLGKATKLPIAIAAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420

Query: 421 FGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE 480
           FGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE
Sbjct: 421 FGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE 480

Query: 481 EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSHV 530
           EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPS V
Sbjct: 481 EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSRV 530

[Close]

BLAST of Chy12G214270 vs. NCBI nr
Match: XP_038895485.1 (high affinity nitrate transporter 2.4-like [Benincasa hispida] >XP_038895611.1 high affinity nitrate transporter 2.4-like [Benincasa hispida])

HSP 1 Score: 1038 bits (2684), Expect = 0.0
Identity = 518/530 (97.74%), Postives = 523/530 (98.68%), Query Frame = 0


Query: 1   MGDVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKSKVFRIWSLANP 60
           MGDVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKF+LPVDSEHK+KVF++WSLANP
Sbjct: 1   MGDVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFSLPVDSEHKAKVFKLWSLANP 60

Query: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120
           HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA
Sbjct: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120

Query: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYITVRFMIGFSLATFVSCQYWMSTMFN 180
           VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYI VRFMIGFSLATFVSCQYWMSTMFN
Sbjct: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFN 180

Query: 181 SKIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGFLHVLMGI 240
           S+IIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGFLHVLMGI
Sbjct: 181 SQIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGFLHVLMGI 240

Query: 241 LVLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDN 300
           LVLTLGQDLPDGNL SLQKKGNV KDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDN
Sbjct: 241 LVLTLGQDLPDGNLGSLQKKGNVTKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDN 300

Query: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT 360
           VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT
Sbjct: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT 360

Query: 361 CGGVFCMWLGKATKLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420
            GGVFCMWLGKA KLP AIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN
Sbjct: 361 FGGVFCMWLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420

Query: 421 FGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE 480
           FGSGLTQLVFFSTTKYSTDTGLF MGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE
Sbjct: 421 FGSGLTQLVFFSTTKYSTDTGLFFMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE 480

Query: 481 EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSHV 530
           EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSHV
Sbjct: 481 EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSHV 530

[Close]

BLAST of Chy12G214270 vs. TAIR 10
Match: AT5G60770.1 (nitrate transporter 2.4 )

HSP 1 Score: 863.6 bits (2230), Expect = 8.2e-251
Identity = 420/530 (79.25%), Postives = 476/530 (89.81%), Query Frame = 0


Query: 1   MGDVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKSKVFRIWSLANP 60
           M D  G PGSSMHGVTGREQ++AFSV SP VP+D++AKF+LPVD+EHK+KVF++ S   P
Sbjct: 1   MADGFGEPGSSMHGVTGREQSYAFSVESPAVPSDSSAKFSLPVDTEHKAKVFKLLSFEAP 60

Query: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120
           HMRTFHL+WISFFTCF+STFAAAPLVPIIRDNLNLT+ D+GNAGV SVSGSIFSRLVMGA
Sbjct: 61  HMRTFHLAWISFFTCFISTFAAAPLVPIIRDNLNLTRQDVGNAGVASVSGSIFSRLVMGA 120

Query: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYITVRFMIGFSLATFVSCQYWMSTMFN 180
           VCDLLGPRYGCAFL+MLSAPTVF MSFV  A GYITVRFMIGF LATFVSCQYWMSTMFN
Sbjct: 121 VCDLLGPRYGCAFLVMLSAPTVFSMSFVGGAGGYITVRFMIGFCLATFVSCQYWMSTMFN 180

Query: 181 SKIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGFLHVLMGI 240
            +IIGLVNGTAAGWGNMGGG TQL+MP++Y++I+R G+T FTAWR+AFF+PG++H++MGI
Sbjct: 181 GQIIGLVNGTAAGWGNMGGGVTQLLMPMVYEIIRRLGSTSFTAWRMAFFVPGWMHIIMGI 240

Query: 241 LVLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDN 300
           LVLTLGQDLPDGN ++L+KKG V KDKFS VLWYA+TNYRTW+FVLLYGYSMGVEL+TDN
Sbjct: 241 LVLTLGQDLPDGNRSTLEKKGAVTKDKFSKVLWYAITNYRTWVFVLLYGYSMGVELTTDN 300

Query: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT 360
           VIAEYF+DRF LKLHTAGIIAA+FGMAN  ARP GG+ASD+AAR FGMRGRLWTLWI+QT
Sbjct: 301 VIAEYFFDRFHLKLHTAGIIAASFGMANFFARPIGGWASDIAARRFGMRGRLWTLWIIQT 360

Query: 361 CGGVFCMWLGKATKLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420
            GG FC+WLG+AT LP A+V MILFS+GAQAACGATF IIPFISRRSLGIISGLTGAGGN
Sbjct: 361 LGGFFCLWLGRATTLPTAVVFMILFSLGAQAACGATFAIIPFISRRSLGIISGLTGAGGN 420

Query: 421 FGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE 480
           FGSGLTQLVFFST+ +ST+ GL  MG+MI+ CTLPVTLVHFPQWGSMFLP T+   KSTE
Sbjct: 421 FGSGLTQLVFFSTSTFSTEQGLTWMGVMIMACTLPVTLVHFPQWGSMFLPSTEDEVKSTE 480

Query: 481 EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSHV 531
           E+YY  EWTE EK+KG+H+ S+KFA NSRSERGRRVASAP+PP   P HV
Sbjct: 481 EYYYMKEWTETEKRKGMHEGSLKFAVNSRSERGRRVASAPSPP---PEHV 527

[Close]

BLAST of Chy12G214270 vs. TAIR 10
Match: AT1G08090.1 (nitrate transporter 2:1 )

HSP 1 Score: 862.1 bits (2226), Expect = 2.4e-250
Identity = 420/530 (79.25%), Postives = 473/530 (89.25%), Query Frame = 0


Query: 1   MGDVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKSKVFRIWSLANP 60
           MGD  G PGSSMHGVTGREQ+FAFSV SPIV TD TAKF LPVD+EHK+ VF+++S A P
Sbjct: 1   MGDSTGEPGSSMHGVTGREQSFAFSVQSPIVHTDKTAKFDLPVDTEHKATVFKLFSFAKP 60

Query: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120
           HMRTFHLSWISF TCFVSTFAAAPLVPIIR+NLNLTK DIGNAGV SVSGSIFSRLVMGA
Sbjct: 61  HMRTFHLSWISFSTCFVSTFAAAPLVPIIRENLNLTKQDIGNAGVASVSGSIFSRLVMGA 120

Query: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYITVRFMIGFSLATFVSCQYWMSTMFN 180
           VCDLLGPRYGCAFL+MLSAPTVF MSFVS+AAG+ITVRFMIGF LATFVSCQYWMSTMFN
Sbjct: 121 VCDLLGPRYGCAFLVMLSAPTVFSMSFVSDAAGFITVRFMIGFCLATFVSCQYWMSTMFN 180

Query: 181 SKIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGFLHVLMGI 240
           S+IIGLVNGTAAGWGNMGGG TQL+MP++Y++I+R G+T FTAWRIAFF+PG+LH++MGI
Sbjct: 181 SQIIGLVNGTAAGWGNMGGGITQLLMPIVYEIIRRCGSTAFTAWRIAFFVPGWLHIIMGI 240

Query: 241 LVLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDN 300
           LVL LGQDLPDGN A+L+K G VAKDKF  +LWYAVTNYRTWIFVLLYGYSMGVELSTDN
Sbjct: 241 LVLNLGQDLPDGNRATLEKAGEVAKDKFGKILWYAVTNYRTWIFVLLYGYSMGVELSTDN 300

Query: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT 360
           VIAEYF+DRF LKLHTAG+IAA FGMAN  ARP GGYASD AA+YFGMRGRLWTLWI+QT
Sbjct: 301 VIAEYFFDRFHLKLHTAGLIAACFGMANFFARPAGGYASDFAAKYFGMRGRLWTLWIIQT 360

Query: 361 CGGVFCMWLGKATKLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420
            GG+FC+WLG+A  L  A+VAM+LFS+GAQAACGATF I+PF+SRR+LGIISGLTGAGGN
Sbjct: 361 AGGLFCVWLGRANTLVTAVVAMVLFSMGAQAACGATFAIVPFVSRRALGIISGLTGAGGN 420

Query: 421 FGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE 480
           FGSGLTQL+FFST+ ++T+ GL  MG+MIV CTLPVTLVHFPQWGSMFLPP+    K TE
Sbjct: 421 FGSGLTQLLFFSTSHFTTEQGLTWMGVMIVACTLPVTLVHFPQWGSMFLPPSTDPVKGTE 480

Query: 481 EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSHV 531
             YYGSEW E EK+K +HQ S++FAEN++SE GRRV SA TPP  TP++V
Sbjct: 481 AHYYGSEWNEQEKQKNMHQGSLRFAENAKSEGGRRVRSAATPPENTPNNV 530

[Close]

BLAST of Chy12G214270 vs. TAIR 10
Match: AT5G60780.1 (nitrate transporter 2.3 )

HSP 1 Score: 791.6 bits (2043), Expect = 4.0e-229
Identity = 391/531 (73.63%), Postives = 451/531 (84.93%), Query Frame = 0


Query: 5   EGSPGSSMHGVTGREQTFAFSV---ASPIVPTDTTAKFALPVDSEHKSKVFRIWSLANPH 64
           EGS G+S+HGVT REQ F+FSV   +  +   D TAKFALPVDSEH++KVF   S A PH
Sbjct: 9   EGSIGTSLHGVTAREQVFSFSVDASSQTVQSDDPTAKFALPVDSEHRAKVFNPLSFAKPH 68

Query: 65  MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAV 124
           MR FHL W+SFFTCF+STFAAAPLVPIIRDNL+LTK DIGNAGV SVSG+IFSRL MGAV
Sbjct: 69  MRAFHLGWLSFFTCFISTFAAAPLVPIIRDNLDLTKTDIGNAGVASVSGAIFSRLAMGAV 128

Query: 125 CDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYITVRFMIGFSLATFVSCQYWMSTMFNS 184
           CDLLG RYG AF +ML+APTVF MSFV   +GY+ VRFMIGF LATFVSCQYW S MFN 
Sbjct: 129 CDLLGARYGTAFSLMLTAPTVFSMSFVGGPSGYLGVRFMIGFCLATFVSCQYWTSVMFNG 188

Query: 185 KIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGFLHVLMGIL 244
           KIIGLVNG A GWG+MGGG TQL+MP+++ VI+ AGATPF AWRIAFF+PGFL V+MGIL
Sbjct: 189 KIIGLVNGCAGGWGDMGGGVTQLLMPMVFHVIKLAGATPFMAWRIAFFVPGFLQVVMGIL 248

Query: 245 VLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV 304
           VL+LGQDLPDGNL++LQK G V+KDKFS V W+AV NYRTWI  +LYG SMG+EL+ +NV
Sbjct: 249 VLSLGQDLPDGNLSTLQKSGQVSKDKFSKVFWFAVKNYRTWILFVLYGSSMGIELTINNV 308

Query: 305 IAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTC 364
           I+ YFYDRF+LKL TAGI+AA+FGMAN +ARPFGGYASDVAAR FGMRGRLWTLWI QT 
Sbjct: 309 ISGYFYDRFNLKLQTAGIVAASFGMANFIARPFGGYASDVAARVFGMRGRLWTLWIFQTV 368

Query: 365 GGVFCMWLGKATKLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 424
           G +FC+WLG+A+ LPIAI+AM+LFSIG QAACGA FG+ PF+SRRSLG+ISGLTGAGGNF
Sbjct: 369 GALFCIWLGRASSLPIAILAMMLFSIGTQAACGALFGVAPFVSRRSLGLISGLTGAGGNF 428

Query: 425 GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEE 484
           GSGLTQL+FFS+ ++ST  GL LMG+M V CTLPV  +HFPQWGSMFL P+   E+S EE
Sbjct: 429 GSGLTQLLFFSSARFSTAEGLSLMGVMAVLCTLPVAFIHFPQWGSMFLRPSTDGERSQEE 488

Query: 485 FYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASA--PTPPNTTPSHV 531
           +YYGSEWTE+EK++GLH+ SIKFAENSRSERGR+VA A  PTP N TPSHV
Sbjct: 489 YYYGSEWTENEKQQGLHEGSIKFAENSRSERGRKVALANIPTPENGTPSHV 539

[Close]

BLAST of Chy12G214270 vs. TAIR 10
Match: AT1G08100.1 (nitrate transporter 2.2 )

HSP 1 Score: 784.6 bits (2025), Expect = 4.9e-227
Identity = 385/516 (74.61%), Postives = 453/516 (87.79%), Query Frame = 0


Query: 1   MGDVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKSKVFRIWSLANP 60
           MG  +  PGSSMHGVTGREQ++AFSV     PT+T  K+ LPVD+E K+ VF+++S A P
Sbjct: 1   MGSTD-EPGSSMHGVTGREQSYAFSVDGS-EPTNTKKKYNLPVDAEDKATVFKLFSFAKP 60

Query: 61  HMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGA 120
           HMRTFHLSWISF TCFVSTFAAAPL+PIIR+NLNLTK DIGNAGV SVSGSIFSRLVMGA
Sbjct: 61  HMRTFHLSWISFSTCFVSTFAAAPLIPIIRENLNLTKHDIGNAGVASVSGSIFSRLVMGA 120

Query: 121 VCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYITVRFMIGFSLATFVSCQYWMSTMFN 180
           VCDLLGPRYGCAFL+MLSAPTVF MSFVS+AAG+ITVRFMIGF LATFVSCQYWMSTMFN
Sbjct: 121 VCDLLGPRYGCAFLVMLSAPTVFSMSFVSDAAGFITVRFMIGFCLATFVSCQYWMSTMFN 180

Query: 181 SKIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGFLHVLMGI 240
           S+IIGLVNGTAAGWGNMGGG TQL+MP++Y++I+R G+T FTAWRIAFF+PG+LH++MGI
Sbjct: 181 SQIIGLVNGTAAGWGNMGGGITQLLMPIVYEIIRRCGSTAFTAWRIAFFVPGWLHIIMGI 240

Query: 241 LVLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDN 300
           LVLTLGQDLP GN A+++K G VAKDKF  +LWYAVTNYRTWIFVLLYGYSMGVELSTDN
Sbjct: 241 LVLTLGQDLPGGNRAAMEKAGEVAKDKFGKILWYAVTNYRTWIFVLLYGYSMGVELSTDN 300

Query: 301 VIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQT 360
           VIAEYF+DRF LKLHTAGIIAA FGMAN  ARP GG+ASD+AA+ FGMRGRLWTLWI+QT
Sbjct: 301 VIAEYFFDRFHLKLHTAGIIAACFGMANFFARPAGGWASDIAAKRFGMRGRLWTLWIIQT 360

Query: 361 CGGVFCMWLGKATKLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGN 420
            GG+FC+WLG+A  L  A+V+M+LFS+GAQAACGATF I+PF+SRR+LGIISGLTGAGGN
Sbjct: 361 SGGLFCVWLGRANTLVTAVVSMVLFSLGAQAACGATFAIVPFVSRRALGIISGLTGAGGN 420

Query: 421 FGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTE 480
           FGSGLTQLVFFST++++T+ GL  MG+MIV CTLPVTL+HFPQWGSMF PP+     +TE
Sbjct: 421 FGSGLTQLVFFSTSRFTTEEGLTWMGVMIVACTLPVTLIHFPQWGSMFFPPSNDSVDATE 480

Query: 481 EFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRV 517
            +Y G E++++E++ G+H +S  FA+ +++E G  V
Sbjct: 481 HYYVG-EYSKEEQQIGMHLKSKLFADGAKTEGGSSV 513

[Close]

BLAST of Chy12G214270 vs. TAIR 10
Match: AT3G45060.1 (high affinity nitrate transporter 2.6 )

HSP 1 Score: 774.2 bits (1998), Expect = 6.6e-224
Identity = 381/534 (71.35%), Postives = 446/534 (83.52%), Query Frame = 0


Query: 5   EGSPGSSMHGVTGREQTFAFSV------ASPIVPTDTTAKFALPVDSEHKSKVFRIWSLA 64
           +GS G+S+HGVT REQ F+FSV      +  +   D TAKFALPVDSEH++KVF+  S A
Sbjct: 9   DGSIGTSLHGVTAREQVFSFSVQEDVPSSQAVRTNDPTAKFALPVDSEHRAKVFKPLSFA 68

Query: 65  NPHMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVM 124
            PHMR FHL WISFFTCF+STFAAAPLVP+IRDNL+LTK DIGNAGV SVSG+IFSRL M
Sbjct: 69  KPHMRAFHLGWISFFTCFISTFAAAPLVPVIRDNLDLTKTDIGNAGVASVSGAIFSRLAM 128

Query: 125 GAVCDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYITVRFMIGFSLATFVSCQYWMSTM 184
           GAVCDLLG RYG AF +ML+AP VF MSFV++A  Y+ VRFMIGF LATFVSCQYW S M
Sbjct: 129 GAVCDLLGARYGTAFSLMLTAPAVFSMSFVADAGSYLAVRFMIGFCLATFVSCQYWTSVM 188

Query: 185 FNSKIIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGFLHVLM 244
           F  KIIGLVNG A GWG+MGGG TQL+MP+++ VI+  GATPFTAWR AFFIPG L ++M
Sbjct: 189 FTGKIIGLVNGCAGGWGDMGGGVTQLLMPMVFHVIKLTGATPFTAWRFAFFIPGILQIVM 248

Query: 245 GILVLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELST 304
           GILVLTLGQDLPDGNL++LQK G V+KDKFS V W+AV NYRTWI  +LYG+SMGVEL+ 
Sbjct: 249 GILVLTLGQDLPDGNLSTLQKSGQVSKDKFSKVFWFAVKNYRTWILFMLYGFSMGVELTI 308

Query: 305 DNVIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWIL 364
           +NVI+ YFYDRF+L LHTAGIIAA+FGMAN  ARPFGGYASDVAAR FGMRGRLW LWIL
Sbjct: 309 NNVISGYFYDRFNLTLHTAGIIAASFGMANFFARPFGGYASDVAARLFGMRGRLWILWIL 368

Query: 365 QTCGGVFCMWLGKATKLPIAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAG 424
           QT G +FC+WLG+A+ LPIAI+AM+LFS+G QAACGA FG+ PF+SRRSLG+ISGLTGAG
Sbjct: 369 QTVGALFCIWLGRASSLPIAILAMMLFSMGTQAACGALFGVAPFVSRRSLGLISGLTGAG 428

Query: 425 GNFGSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKS 484
           GNFGSG+TQL+FFS++++ST  GL LMG+M V C+LPV  +HFPQWGSMFL P++  EKS
Sbjct: 429 GNFGSGVTQLLFFSSSRFSTAEGLSLMGVMAVVCSLPVAFIHFPQWGSMFLRPSQDGEKS 488

Query: 485 TEEFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRR--VASAPTPPNTTPSHV 531
            EE YYG+EWTE+EK  GLH+ SIKFAENSRSERGR+  +A  PTP   +P+HV
Sbjct: 489 KEEHYYGAEWTEEEKSLGLHEGSIKFAENSRSERGRKAMLADIPTPETGSPAHV 542

The following BLAST results are available for this feature:

BLAST of Chy12G214270 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FJH8	1.2e-249	79.25	High affinity nitrate transporter 2.4 OS=Arabidopsis thaliana OX=3702 GN=NRT2.4 ...	[more]
O82811	3.4e-249	79.25	High-affinity nitrate transporter 2.1 OS=Arabidopsis thaliana OX=3702 GN=NRT2.1 ...	[more]
P0DKG9	3.0e-237	76.94	High-affinity nitrate transporter 2.1 OS=Oryza sativa subsp. japonica OX=39947 G...	[more]
P0DKH0	3.0e-237	76.94	High-affinity nitrate transporter 2.2 OS=Oryza sativa subsp. japonica OX=39947 G...	[more]
Q9FJH7	5.6e-228	73.63	High affinity nitrate transporter 2.3 OS=Arabidopsis thaliana OX=3702 GN=NRT2.3 ...	[more]

BLAST of Chy12G214270 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A3Q9CZ42	6.1e-302	98.68	High affinity nitrate transporter 2.1 OS=Cucumis sativus OX=3659 GN=NRT2.1 PE=2 ...	[more]
Q6PRP8	2.3e-301	98.68	High-affinity nitrate transporter OS=Cucumis sativus OX=3659 GN=NRT2 PE=2 SV=3	[more]
S4VM42	3.0e-301	98.30	High-affinity nitrate transporter NRT2.2 OS=Cucumis sativus OX=3659 GN=NRT2.2 PE...	[more]
A0A6J1DHB7	4.4e-292	94.71	high affinity nitrate transporter 2.4-like OS=Momordica charantia OX=3673 GN=LOC...	[more]
A0A6J1HIH1	5.7e-292	95.09	high affinity nitrate transporter 2.4-like OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]

BLAST of Chy12G214270 vs. NCBI nr


Match Name	Identity	Description
XP_031736393.1	98.68	high affinity nitrate transporter 2.4-like isoform X1 [Cucumis sativus] >AZP5371...	[more]
NP_001274401.1	98.68	high affinity nitrate transporter 2.4-like [Cucumis sativus] >AAS93686.3 high-af...	[more]
AGO64298.1	98.30	high-affinity nitrate transporter NRT2.2 [Cucumis sativus]	[more]
NP_001269063.2	98.11	high affinity nitrate transporter 2.4-like [Cucumis sativus]	[more]
XP_038895485.1	97.74	high affinity nitrate transporter 2.4-like [Benincasa hispida] >XP_038895611.1 h...	[more]

BLAST of Chy12G214270 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G60770.1	8.2e-251	79.25	nitrate transporter 2.4	[more]
AT1G08090.1	2.4e-250	79.25	nitrate transporter 2:1	[more]
AT5G60780.1	4.0e-229	73.63	nitrate transporter 2.3	[more]
AT1G08100.1	4.9e-227	74.61	nitrate transporter 2.2	[more]
AT3G45060.1	6.6e-224	71.35	high affinity nitrate transporter 2.6	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036259	MFS transporter superfamily	GENE3D	1.20.1250.20	MFS general substrate transporter like domains	coord: 264..466 e-value: 3.9E-27 score: 97.0
IPR036259	MFS transporter superfamily	GENE3D	1.20.1250.20	MFS general substrate transporter like domains	coord: 58..254 e-value: 3.1E-21 score: 77.7
IPR036259	MFS transporter superfamily	SUPERFAMILY	103473	MFS general substrate transporter	coord: 57..460
IPR011701	Major facilitator superfamily	PFAM	PF07690	MFS_1	coord: 69..425 e-value: 3.8E-20 score: 72.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 490..513
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 490..530
None	No IPR available	PANTHER	PTHR23515:SF16	HIGH-AFFINITY NITRATE TRANSPORTER 2.1-LIKE	coord: 1..527
IPR044772	Nitrate transporter	PANTHER	PTHR23515	HIGH-AFFINITY NITRATE TRANSPORTER 2.3	coord: 1..527
IPR044772	Nitrate transporter	CDD	cd17341	MFS_NRT2_like	coord: 65..458 e-value: 8.11605E-136 score: 397.007
IPR020846	Major facilitator superfamily domain	PROSITE	PS50850	MFS	coord: 64..458 score: 8.889123

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy12G214270.1	Chy12G214270.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071249	cellular response to nitrate
biological_process	GO:0042128	nitrate assimilation
biological_process	GO:0015706	nitrate transport
biological_process	GO:0031204	posttranslational protein targeting to membrane, translocation
biological_process	GO:0055085	transmembrane transport
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0009705	plant-type vacuole membrane
cellular_component	GO:0005886	plasma membrane
cellular_component	GO:0071261	Ssh1 translocon complex
molecular_function	GO:0015112	nitrate transmembrane transporter activity
molecular_function	GO:0008320	protein transmembrane transporter activity
molecular_function	GO:0022857	transmembrane transporter activity