Chy12G214100 (gene) Cucumber (hystrix) v1

Overview
NameChy12G214100
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
Descriptionvacuolar protein sorting-associated protein 24 homolog 1
LocationchrH12: 13154537 .. 13159259 (+)
RNA-Seq ExpressionChy12G214100
SyntenyChy12G214100
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAAAAGTGATGAACATCTTGAAGCCCAAACCAAATCCTCAACAACAACTGAGGGATTGGCAGCGTAGGCTCCGACAAGAGTGCCGCAACGTCGAACGCCAAATTCGAGGTAACGGATCGTATTTTCGTATCTTCAGTTTCAATCTTTTTCGTGTTTTCTTTTATGTATCTGTTCCTTGCTAGGTTAAAATTCTGATCATGCCATCCATTCTTCTTCTACCGTTAGGGATTCTTTAATTCTTCATGTCTCTTTTCTTTGATTGATTTGGGTCAGATATACAGCGAGAAGAGAAAAATGTGCAGAAAGCCATTCGGGATGCTGCCAAGAGGAATGATATGGTCTCTGCAAAGGTATGTTAAGAACTTGAGGTTATCTCTGTTTAATTTTTTTATTAGAGGTTTCATAATGAAAAACAAATTTCGATCTGGGAAATTTTCTACAAGTTAAGAATATTTAGGGTATTGAGGTGAAAAAAGGGCGAATCTGAAAAATATTAACCAAAAATGTTATATGAAAACTTATTTTCACTAATGTTCTTTTATTTCGGGAACAAAACCAGGGGAGTAGTCTGTTCCCTCCCTTGTTTGTTCTTGTGTTTTAGTATAAATCTTATGCCTTGAGGGCAAAGCATGATTTGAAAATTGGGGGTGGGGTTTGGTTGTGAGAGAATGTGCAGGTTTGCCTATAGCTATTACTACTGTTGCTAAGGCATTATGAAATAAGCCTTCCGACATTTGGAATGATGCCTTAGATCAACTTAAAAGTGGATAACCATCTTATTCATTAATGCACGACGTTTACAACTCTAGAATGAAAAAGGAGACCTTTGTTAGATAACATGCCCTAGATATGGTGGTTCATTTTATAGTTTTGCTGTAAAAATGGAAAAAAATTAGGTTCAGAGTAATAGAATCTCACTTTTCTCTCATCCTTCGTATATCCTTTTTAAAACTTTAATGATAAGTATCTACCACTAATTAAGAATATATATATGGAATGTCCTACAGCCCCTGCTCGTAATGCATACCAAGCTGTTAGGGATACCGCCGGATAAAGGCCAATGATAAAGCTCGAGTCTATATCTTAGCAAGTCTGTCTGCAGGGTTTTGGTTTTATTGACAGGAAAACTAAATTGCATGAAATTAAAGTGCACAATAAACTATTGACCATAGTATGAATGCTTGAATTTCATGTAGTGATGTGAGAGTGGATCAAGTTCAAGTTGATAGTCTAAATGGTCTATAGTATAGGAATAGGTAATCACTTTGGGCTGAGGGTTATTTTTTCTTAATTTTATAAAAGCTACATCTTTCATGGACAAATATTGCAAGGGTATACACAAAAAGCCAAGCCCCCTAAAAGAGAAACCAAACTTCACTCGGCTCCTTATCCTAAACACTGTTATTCCACTCGTCCAAAAATACTCCAAATTAATATGCACCCAGCAAATCATAAAAGTTGGTCCTTGTCACGCTGAAGTGGATGGGATGAACTCTCCAATCAACTATAATCATTTACATTGCTGAATTTCTTTTTGTTCCACTGGTACCTTAGATGGCTGGGTTCAGTTTGATATTGAGGTTTCCTATATATCTGGAATGTATTTATGTGATATTATCTAAATTCTCAGTGGACTAGGGAAAATGATCTATAATTCAGTACACTGGACCTAATCTCTAATTGTTTCAGCATGGAATTATGTTCTATCTCTTGCTTAATATTGCCATTTATATGTGTTTCAGGCACTGGCTAAAGAAATTGTGAGGTCTAAAAAAACTGTGAACCGCCTTCATGAAAACAAGGCACAGCTTAATTCGATATCCATGCACCTTGGCGAAAGTGTTGGTACGATGTTTTGCATATTCTAAAGCTTGAAAATTCTTCTAGGACCCTTTGCTGTTTTATCTTCAACAACCTAGCTAAATGCTTTGTGGAGTCTGAGAGATAATTTGATGATTATGAATCCATGACAAACTCTGTAATATAGCTAAAGTTTATTGTAAACTATTGATTTTATGAAATTAATCTCTATTTCATTAATTATTCTAACCATAGAGAAATACACTTGAGAAAATAATATCAAATAATATCTCCAGAATAATATCACCTAAAAATATTCTAATTATATTAATACCCTCCCTCAAACTCAAGGTTGAAATCACAAACTTGAGTTTGCTAAGAACTAAGAAAAGAAACAATAGATCTAGAATAGAATAAAAAACCCGAAGAACAAACACATAAAGAACTTCTAGGATAAAACAAACACACGAAGAGCGAAACAAAGAACTTCTGGGATAGAAACATAGATCCTCGTATAGAGATCTATAACAGAACCTAGGAAGGACGAAACAAGAACTCTGGGGGCTAAGTGAATGGAGCAAAACTGAAGCAAAGTAGAATCAAAGTAGGACGAAAACAAGGTCTGAATTGGGCAGGAGTGTTTCGTCGAATCAAAATGGAATCGAATGAACTAAACTAGGGTCTGAATTGGGTAGGAGTGTTTCGTCGGATCAGAATGGAACCGAACGAACTAATCTAGAGCAAGTCAGAATCAGACTGAACGAGAAATTTGGGGTGAACAGAACGAAAACTGAACAGAGCTGATAGGTTGAGCGTATAGAGGGATTTGCAGTGTTCTTCGCAAAAAGAGAAGATGGGTTTCGTGGCGGCGAGCTTCACGAACGGAGGCGGACGGAGCTGAACCTGTGCGTCGTCGTCTGTGAGTGGACCGGAGCGGTGGATCATGGATCTGAACAAAGATTCACCTTCTTCATCAGGATTGGGAATCTTCAACCGAAAGGAGTCGTCTGGTACGAGTAAGGGGCCGCGGCAGCGGGTCGGTCTAATATGGAACAGTATGGTTCGAAGGCGGAAGATGAAATAAGACACGGATCTGGAAGTTGGAGACACGAACGGAGATGCGCGAACAAATCGGTGGTTGCAATGTGCGAACAGATAGATCTGAGTGAGGACGAATGGATACATGGATCTGGGTGCGAGAGTGCGACGAATTGTGTGGATGGAAGGAGCAGTGGTGGCGGCAGTCTCGGCGGAAGGAGTAGTGGTGGTGGCGGCTGCAGAATCAGAGACAAGGATTTTGATCTATGCGGTAGAGCGAACTACTTGGAGGAATGATCTGTATTGATTACTGATGGGTATGGCTCTGCCTCCGTCGACGAATCAATGGAGGCAGACGAACAGGGATGTGACGGGAATACAAGGAGACTAAAACCTATAGCTTTGATACCATGTTAATTTTATGGAATTAATCTCTATTTTATTAATTATTTAGTAGAGGTACAAGCCTATATATAACCATAGAGAAATACACTTAAATAATATCAAATAATATCTTCAGAATTATATCACCTAAGAATAATCTAATTATATTAATATAAACAATAGTTTGCTGAAGACATACATGCATGCATGCATATATATATGTAAAGATATATTTTTTGCTTATGTTATCAATTGAATACAATAGAAAAGCTTGAATTGGTATGAAATTTAGAAGTATAAGGATGGAAGGTGGCGGAAGGGATTCTTACTTAGCTCAATTTACGACAACTTTTTATATCGTAGTGCAACATAATATTACCATTGTTGAAAGTCTACCAATTTTGCAGCCATTGCGAGAACGGTGGGACATCTTTCCAAGAGTGCTGAAGTTATGAAGCTTGTTAATAATCTGATGAAGGCCCCAGAAATGGCAAAAACAATGCAAGAGTTTAGCAAGGAGATGACCAAGGTATCTTTTTTAAGAATATAAACAATTTCTTACTTTCTCCTTGGTCAAGGCTATGAATTTAGAGGCTTGGAGCTGACATAAGACTTGATTGAACATTATTTGTTTGGTTCTTTTTTGTGGGTAGAAATCATTTTTTATCAAGTTACAATGTGTTAATCCTGGTTCCATAACTTGGTTTATCTTTTATTGTCATTGGGAGAATGAGGAAATAATTCCACGATTATACTATCTTTTATTTATAGAAACATAGTTAACTTTGTTTGACACCAAAAGGAAAAAGCCTAAGGTTTGAGGTGGAGAGAACCTCCTCCCAAAGAGACTACAAGAGAGAGCCTACTAATTGTACAAAATTATGAGGAGTCTATAATTACAAAAGGATGTTTTGTGGTTGGAGCTCCAACACAACTTTGTTTACGACCCGTTTGTTAAATCCTCACGCTCCTTTGTTTGTGAACATTTATCGAAAGGATACTTGCTTGTAGCCTTATTGTCAAGTTTTTGTTCTCTGTTTAACTTACCAATTGCCACTAAATTTATATGTTTTCTTTAGAAGGTGTTATAATACCATTATTAATGTAGCTAGTTTTTTATATACTATATCTATAGGCTGGGGTGATTGAGGAATTTGTAAATGATGCGGTGGATTCAGCACTAGATTCTGAGGATATTGAAGAGGAGATTGAAGAGGAAGTTGACAAAGTTTTGACTGCCATTGCCGGTGAGACTGTGGCACAGCTTCCAGAAGCTGTCAGGAAGGAAAAGATGAAACAACCGGCTCATGTGCAGGAAGTCCGGGAAGAGGTTTCTATTGCATAATTTTGAGCACCCACGTTCTAGTCCTACGATGGTTATGTACAGGAAAAGTATTGTGACATTTATTTCTTTTGCAGGAAGCAATAGCTGAGGGCGTTGATGACGAGGAAGAACTAGAAGAAATAAGGGCTCGACTTGCGAAAGTTAGATCATAA

mRNA sequence

ATGGAAAAAGTGATGAACATCTTGAAGCCCAAACCAAATCCTCAACAACAACTGAGGGATTGGCAGCGTAGGCTCCGACAAGAGTGCCGCAACGTCGAACGCCAAATTCGAGATATACAGCGAGAAGAGAAAAATGTGCAGAAAGCCATTCGGGATGCTGCCAAGAGGAATGATATGGTCTCTGCAAAGGCACTGGCTAAAGAAATTGTGAGGTCTAAAAAAACTGTGAACCGCCTTCATGAAAACAAGGCACAGCTTAATTCGATATCCATGCACCTTGGCGAAAGTGTTGCCATTGCGAGAACGGTGGGACATCTTTCCAAGAGTGCTGAAGTTATGAAGCTTGTTAATAATCTGATGAAGGCCCCAGAAATGGCAAAAACAATGCAAGAGTTTAGCAAGGAGATGACCAAGGCTGGGGTGATTGAGGAATTTGTAAATGATGCGGTGGATTCAGCACTAGATTCTGAGGATATTGAAGAGGAGATTGAAGAGGAAGTTGACAAAGTTTTGACTGCCATTGCCGGTGAGACTGTGGCACAGCTTCCAGAAGCTGTCAGGAAGGAAAAGATGAAACAACCGGCTCATGTGCAGGAAGTCCGGGAAGAGGAAGCAATAGCTGAGGGCGTTGATGACGAGGAAGAACTAGAAGAAATAAGGGCTCGACTTGCGAAAGTTAGATCATAA

Coding sequence (CDS)

ATGGAAAAAGTGATGAACATCTTGAAGCCCAAACCAAATCCTCAACAACAACTGAGGGATTGGCAGCGTAGGCTCCGACAAGAGTGCCGCAACGTCGAACGCCAAATTCGAGATATACAGCGAGAAGAGAAAAATGTGCAGAAAGCCATTCGGGATGCTGCCAAGAGGAATGATATGGTCTCTGCAAAGGCACTGGCTAAAGAAATTGTGAGGTCTAAAAAAACTGTGAACCGCCTTCATGAAAACAAGGCACAGCTTAATTCGATATCCATGCACCTTGGCGAAAGTGTTGCCATTGCGAGAACGGTGGGACATCTTTCCAAGAGTGCTGAAGTTATGAAGCTTGTTAATAATCTGATGAAGGCCCCAGAAATGGCAAAAACAATGCAAGAGTTTAGCAAGGAGATGACCAAGGCTGGGGTGATTGAGGAATTTGTAAATGATGCGGTGGATTCAGCACTAGATTCTGAGGATATTGAAGAGGAGATTGAAGAGGAAGTTGACAAAGTTTTGACTGCCATTGCCGGTGAGACTGTGGCACAGCTTCCAGAAGCTGTCAGGAAGGAAAAGATGAAACAACCGGCTCATGTGCAGGAAGTCCGGGAAGAGGAAGCAATAGCTGAGGGCGTTGATGACGAGGAAGAACTAGAAGAAATAAGGGCTCGACTTGCGAAAGTTAGATCATAA

Protein sequence

MEKVMNILKPKPNPQQQLRDWQRRLRQECRNVERQIRDIQREEKNVQKAIRDAAKRNDMVSAKALAKEIVRSKKTVNRLHENKAQLNSISMHLGESVAIARTVGHLSKSAEVMKLVNNLMKAPEMAKTMQEFSKEMTKAGVIEEFVNDAVDSALDSEDIEEEIEEEVDKVLTAIAGETVAQLPEAVRKEKMKQPAHVQEVREEEAIAEGVDDEEELEEIRARLAKVRS*
Homology
BLAST of Chy12G214100 vs. ExPASy Swiss-Prot
Match: Q9FFB3 (Vacuolar protein sorting-associated protein 24 homolog 1 OS=Arabidopsis thaliana OX=3702 GN=VPS24-1 PE=1 SV=1)

HSP 1 Score: 348.6 bits (893), Expect = 5.4e-95
Identity = 193/229 (84.28%), Postives = 217/229 (94.76%), Query Frame = 0

Query: 1   MEKVMNILKPKPNPQQQLRDWQRRLRQECRNVERQIRDIQREEKNVQKAIRDAAKRNDMV 60
           ME+VMNI+KPKP+P+Q LRDWQR+LRQECRN+ERQIRDIQ+EE+NVQKAI++AAKRNDMV
Sbjct: 1   MERVMNIIKPKPDPKQLLRDWQRKLRQECRNIERQIRDIQKEERNVQKAIKEAAKRNDMV 60

Query: 61  SAKALAKEIVRSKKTVNRLHENKAQLNSISMHLGESVAIARTVGHLSKSAEVMKLVNNLM 120
           SAKALAKEIV S++TVNRL+ENKAQ+NSISMHLGESVAIARTVGHLSKSAEVMKLVNNLM
Sbjct: 61  SAKALAKEIVSSRRTVNRLYENKAQMNSISMHLGESVAIARTVGHLSKSAEVMKLVNNLM 120

Query: 121 KAPEMAKTMQEFSKEMTKAGVIEEFVNDAVDSALDSEDIEEEIEEEVDKVLTAIAGETVA 180
           KAP+MA TMQEFSKEMTKAGVIEEFVN+A+D+ALDSED+EEEI+EEVDKVLTAIAGET A
Sbjct: 121 KAPQMAATMQEFSKEMTKAGVIEEFVNEAIDNALDSEDMEEEIDEEVDKVLTAIAGETAA 180

Query: 181 QLPEAVRKEKMKQPAH-VQEVREEEAIAEGVDDEEELEEIRARLAKVRS 229
           +LP AVRKE++K PA      REEEA+AEGVDDEEELEEIRARLAKVRS
Sbjct: 181 ELPVAVRKERIKVPAQKASTSREEEAVAEGVDDEEELEEIRARLAKVRS 229

BLAST of Chy12G214100 vs. ExPASy Swiss-Prot
Match: Q9LXH5 (Putative vacuolar protein sorting-associated protein 24 homolog 2 OS=Arabidopsis thaliana OX=3702 GN=VPS24-2 PE=3 SV=1)

HSP 1 Score: 252.7 bits (644), Expect = 4.0e-66
Identity = 147/198 (74.24%), Postives = 171/198 (86.36%), Query Frame = 0

Query: 32  VERQIRDIQREEKNVQKAIRDAAKRNDMVSAKALAKEIVRSKKTVNRLHENKAQLNSISM 91
           ++  + DI+REE+NV KAI+D+ KRND+V+AKALA+EIV S++TV RL+ENKAQ+NSISM
Sbjct: 3   IKSLLSDIEREERNVHKAIKDSVKRNDLVTAKALAREIVSSRRTVKRLYENKAQVNSISM 62

Query: 92  HLGESVAIARTVGHLSKSAEVMKLVNNLMKAPEMAKTMQEFSKEMTKAGVIEEFVNDAVD 151
           HLGES+A A TVG+LSKS EVMKLVN+LMKAPE+A TMQ FSKEMTK GVIEEFV+DAVD
Sbjct: 63  HLGESIATAVTVGNLSKSGEVMKLVNSLMKAPEIAVTMQAFSKEMTKTGVIEEFVSDAVD 122

Query: 152 SALDSEDIEEEIEEEVDKVLTAIAGETVAQLPEAVRKEKMKQPAHVQE-VREEEAIAEGV 211
           +ALDSEDIEEEIEEEVDKVLTAIAGET A+LPEAVRK+KM  PA   E +  E+AI EGV
Sbjct: 123 NALDSEDIEEEIEEEVDKVLTAIAGETAAELPEAVRKQKMNVPAQKAETLHGEDAIVEGV 182

Query: 212 DDEEELEEIRARLAKVRS 229
           D EEELE IR RLA VRS
Sbjct: 183 DAEEELEAIRYRLANVRS 200

BLAST of Chy12G214100 vs. ExPASy Swiss-Prot
Match: Q9CQ10 (Charged multivesicular body protein 3 OS=Mus musculus OX=10090 GN=Chmp3 PE=1 SV=3)

HSP 1 Score: 145.2 bits (365), Expect = 9.1e-34
Identity = 90/216 (41.67%), Postives = 140/216 (64.81%), Query Frame = 0

Query: 14  PQQQLRDWQRRLRQECRNVERQIRDIQREEKNVQKAIRDAAKRNDMVSAKALAKEIVRSK 73
           P++ + +W  ++R+E R V+RQIRDIQREE+ V+++++DAAK+        LAKE++RS+
Sbjct: 12  PKELVNEWSLKIRKEMRVVDRQIRDIQREEEKVKRSVKDAAKKGQKEVCVVLAKEMIRSR 71

Query: 74  KTVNRLHENKAQLNSISMHLGESVAIARTVGHLSKSAEVMKLVNNLMKAPEMAKTMQEFS 133
           K V++L+ +KA +NS+ M +   +A+ R  G L KS EVMK + +L+K PE+  TM+E S
Sbjct: 72  KAVSKLYASKAHMNSVLMGMKNQLAVLRVAGSLQKSTEVMKAMQSLVKIPEIQATMRELS 131

Query: 134 KEMTKAGVIEEFVNDAVDSALDSEDIEEEIEEEVDKVLTAIAGETVAQLPEAVRKEKMK- 193
           KEM KAG+IEE + D  +S  D E++EE  E E+D++L  I    + + P  V     + 
Sbjct: 132 KEMMKAGIIEEMLEDTFESMDDQEEMEEAAEMEIDRILFEITAGALGKAPSKVTDALPEP 191

Query: 194 QPAHVQEVREEEAIAEGVDDEEELEEIRARLAKVRS 229
           +PA      EE    E  +DEE+LE +++RLA +RS
Sbjct: 192 EPAGAMAASEE---GEEEEDEEDLEAMQSRLATLRS 224

BLAST of Chy12G214100 vs. ExPASy Swiss-Prot
Match: Q8CGS4 (Charged multivesicular body protein 3 OS=Rattus norvegicus OX=10116 GN=Chmp3 PE=1 SV=3)

HSP 1 Score: 144.8 bits (364), Expect = 1.2e-33
Identity = 89/215 (41.40%), Postives = 140/215 (65.12%), Query Frame = 0

Query: 14  PQQQLRDWQRRLRQECRNVERQIRDIQREEKNVQKAIRDAAKRNDMVSAKALAKEIVRSK 73
           P++ + +W  ++R+E R V+RQIRDIQREE+ V+++++DAAK+        LAKE++RS+
Sbjct: 12  PKELVNEWSLKIRKEMRVVDRQIRDIQREEEKVKRSVKDAAKKGQKEVCVVLAKEMIRSR 71

Query: 74  KTVNRLHENKAQLNSISMHLGESVAIARTVGHLSKSAEVMKLVNNLMKAPEMAKTMQEFS 133
           K V++L+ +KA +NS+ M +   +A+ R  G L KS EVMK + +L+K PE+  TM+E S
Sbjct: 72  KAVSKLYASKAHMNSVLMGMKNQLAVLRVAGSLQKSTEVMKAMQSLVKIPEIQATMRELS 131

Query: 134 KEMTKAGVIEEFVNDAVDSALDSEDIEEEIEEEVDKVLTAIAGETVAQLPEAVRKEKMKQ 193
           KEM KAG+IEE + D  +S  D E++EE  E E+D++L  I    + + P  V  + + +
Sbjct: 132 KEMMKAGIIEEMLEDTFESMDDQEEMEEAAEMEIDRILFEITAGALGKAPSKV-TDALPE 191

Query: 194 PAHVQEVREEEAIAEGVDDEEELEEIRARLAKVRS 229
           P     +   E   E  DDEE+LE +++RLA +RS
Sbjct: 192 PEPAGAMAASEGDEE--DDEEDLEAMQSRLATLRS 223

BLAST of Chy12G214100 vs. ExPASy Swiss-Prot
Match: Q5RAU5 (Charged multivesicular body protein 3 OS=Pongo abelii OX=9601 GN=CHMP3 PE=2 SV=3)

HSP 1 Score: 142.5 bits (358), Expect = 5.9e-33
Identity = 88/216 (40.74%), Postives = 140/216 (64.81%), Query Frame = 0

Query: 14  PQQQLRDWQRRLRQECRNVERQIRDIQREEKNVQKAIRDAAKRNDMVSAKALAKEIVRSK 73
           P++ + +W  ++R+E R V+RQIRDIQREE+ V+++++DAAK+        LAKE++RS+
Sbjct: 12  PKELVNEWSLKIRKEMRVVDRQIRDIQREEEKVKRSVKDAAKKGQKDVCVVLAKEMIRSR 71

Query: 74  KTVNRLHENKAQLNSISMHLGESVAIARTVGHLSKSAEVMKLVNNLMKAPEMAKTMQEFS 133
           K V++L+ +KA +NS+ M +   +A+ R  G L KS EVMK + +L+K PE+  TM+E S
Sbjct: 72  KAVSKLYASKAHMNSVLMGMKNQLAVLRVAGSLQKSTEVMKAMQSLVKIPEIQATMRELS 131

Query: 134 KEMTKAGVIEEFVNDAVDSALDSEDIEEEIEEEVDKVLTAIAGETVAQLPEAVRKEKMK- 193
           KEM KAG+IEE + D  +S  D E++EEE E E+D++L  I    + + P  V     + 
Sbjct: 132 KEMMKAGIIEEMLEDTFESMDDQEEMEEEAEMEIDRILFEITAGALGKAPSKVTDALPEP 191

Query: 194 QPAHVQEVREEEAIAEGVDDEEELEEIRARLAKVRS 229
           +P+      E+E      ++EE LE +++RLA +RS
Sbjct: 192 EPSGAMAASEDEE-----EEEEALEAMQSRLATLRS 222

BLAST of Chy12G214100 vs. ExPASy TrEMBL
Match: A0A0A0LXU7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G109340 PE=4 SV=1)

HSP 1 Score: 404.8 bits (1039), Expect = 2.3e-109
Identity = 226/228 (99.12%), Postives = 227/228 (99.56%), Query Frame = 0

Query: 1   MEKVMNILKPKPNPQQQLRDWQRRLRQECRNVERQIRDIQREEKNVQKAIRDAAKRNDMV 60
           MEKVMNILKPKPNPQQQLRDWQRRLRQECRNVERQIRDIQREEKNVQKAIRDAAKRNDMV
Sbjct: 1   MEKVMNILKPKPNPQQQLRDWQRRLRQECRNVERQIRDIQREEKNVQKAIRDAAKRNDMV 60

Query: 61  SAKALAKEIVRSKKTVNRLHENKAQLNSISMHLGESVAIARTVGHLSKSAEVMKLVNNLM 120
           SAKALAKEIVRSKKTVNRLHENKAQLNSISMHLGESVAIARTVGHLSKSAEVMKLVNNLM
Sbjct: 61  SAKALAKEIVRSKKTVNRLHENKAQLNSISMHLGESVAIARTVGHLSKSAEVMKLVNNLM 120

Query: 121 KAPEMAKTMQEFSKEMTKAGVIEEFVNDAVDSALDSEDIEEEIEEEVDKVLTAIAGETVA 180
           KAPEMAKTMQEFSKEMTKAGVIEEFVNDAVDSALDSEDIEEEIEEEVDKVLTAIAGET A
Sbjct: 121 KAPEMAKTMQEFSKEMTKAGVIEEFVNDAVDSALDSEDIEEEIEEEVDKVLTAIAGETAA 180

Query: 181 QLPEAVRKEKMKQPAHVQEVREEEAIAEGVDDEEELEEIRARLAKVRS 229
           QLPEAVRKEKMKQPAHVQEVREEEAIAEG+DDEEELEEIRARLAKVRS
Sbjct: 181 QLPEAVRKEKMKQPAHVQEVREEEAIAEGIDDEEELEEIRARLAKVRS 228

BLAST of Chy12G214100 vs. ExPASy TrEMBL
Match: A0A5D3DDQ8 (Vacuolar protein sorting-associated protein 24-like protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold609G001070 PE=4 SV=1)

HSP 1 Score: 402.1 bits (1032), Expect = 1.5e-108
Identity = 225/228 (98.68%), Postives = 226/228 (99.12%), Query Frame = 0

Query: 1   MEKVMNILKPKPNPQQQLRDWQRRLRQECRNVERQIRDIQREEKNVQKAIRDAAKRNDMV 60
           MEK+MNILKPKPNPQQQLRDWQRRLRQECRNVERQIRDIQREEKNV KAIRDAAKRNDMV
Sbjct: 1   MEKMMNILKPKPNPQQQLRDWQRRLRQECRNVERQIRDIQREEKNVHKAIRDAAKRNDMV 60

Query: 61  SAKALAKEIVRSKKTVNRLHENKAQLNSISMHLGESVAIARTVGHLSKSAEVMKLVNNLM 120
           SAKALAKEIVRSKKTVNRLHENKAQLNSISMHLGESVAIARTVGHLSKSAEVMKLVNNLM
Sbjct: 61  SAKALAKEIVRSKKTVNRLHENKAQLNSISMHLGESVAIARTVGHLSKSAEVMKLVNNLM 120

Query: 121 KAPEMAKTMQEFSKEMTKAGVIEEFVNDAVDSALDSEDIEEEIEEEVDKVLTAIAGETVA 180
           KAPEMAKTMQEFSKEMTKAGVIEEFVNDAVDSALDSEDIEEEIEEEVDKVLTAIAGET A
Sbjct: 121 KAPEMAKTMQEFSKEMTKAGVIEEFVNDAVDSALDSEDIEEEIEEEVDKVLTAIAGETAA 180

Query: 181 QLPEAVRKEKMKQPAHVQEVREEEAIAEGVDDEEELEEIRARLAKVRS 229
           QLPEAVRKEKMKQPAHVQEVREEEAIAEGVDDEEELEEIRARLAKVRS
Sbjct: 181 QLPEAVRKEKMKQPAHVQEVREEEAIAEGVDDEEELEEIRARLAKVRS 228

BLAST of Chy12G214100 vs. ExPASy TrEMBL
Match: A0A1S3CIN5 (vacuolar protein sorting-associated protein 24 homolog 1 OS=Cucumis melo OX=3656 GN=LOC103501246 PE=4 SV=1)

HSP 1 Score: 402.1 bits (1032), Expect = 1.5e-108
Identity = 225/228 (98.68%), Postives = 226/228 (99.12%), Query Frame = 0

Query: 1   MEKVMNILKPKPNPQQQLRDWQRRLRQECRNVERQIRDIQREEKNVQKAIRDAAKRNDMV 60
           MEK+MNILKPKPNPQQQLRDWQRRLRQECRNVERQIRDIQREEKNV KAIRDAAKRNDMV
Sbjct: 1   MEKMMNILKPKPNPQQQLRDWQRRLRQECRNVERQIRDIQREEKNVHKAIRDAAKRNDMV 60

Query: 61  SAKALAKEIVRSKKTVNRLHENKAQLNSISMHLGESVAIARTVGHLSKSAEVMKLVNNLM 120
           SAKALAKEIVRSKKTVNRLHENKAQLNSISMHLGESVAIARTVGHLSKSAEVMKLVNNLM
Sbjct: 61  SAKALAKEIVRSKKTVNRLHENKAQLNSISMHLGESVAIARTVGHLSKSAEVMKLVNNLM 120

Query: 121 KAPEMAKTMQEFSKEMTKAGVIEEFVNDAVDSALDSEDIEEEIEEEVDKVLTAIAGETVA 180
           KAPEMAKTMQEFSKEMTKAGVIEEFVNDAVDSALDSEDIEEEIEEEVDKVLTAIAGET A
Sbjct: 121 KAPEMAKTMQEFSKEMTKAGVIEEFVNDAVDSALDSEDIEEEIEEEVDKVLTAIAGETAA 180

Query: 181 QLPEAVRKEKMKQPAHVQEVREEEAIAEGVDDEEELEEIRARLAKVRS 229
           QLPEAVRKEKMKQPAHVQEVREEEAIAEGVDDEEELEEIRARLAKVRS
Sbjct: 181 QLPEAVRKEKMKQPAHVQEVREEEAIAEGVDDEEELEEIRARLAKVRS 228

BLAST of Chy12G214100 vs. ExPASy TrEMBL
Match: A0A6J1DJ20 (vacuolar protein sorting-associated protein 24 homolog 1 OS=Momordica charantia OX=3673 GN=LOC111020496 PE=4 SV=1)

HSP 1 Score: 383.3 bits (983), Expect = 7.3e-103
Identity = 217/229 (94.76%), Postives = 224/229 (97.82%), Query Frame = 0

Query: 1   MEKVMNILKPKPNPQQQLRDWQRRLRQECRNVERQIRDIQREEKNVQKAIRDAAKRNDMV 60
           MEK+M++LKPK NPQQQLRDWQRRLRQECRNVERQIRDIQREEKNVQKAIR+AAKRNDMV
Sbjct: 1   MEKMMSMLKPKANPQQQLRDWQRRLRQECRNVERQIRDIQREEKNVQKAIREAAKRNDMV 60

Query: 61  SAKALAKEIVRSKKTVNRLHENKAQLNSISMHLGESVAIARTVGHLSKSAEVMKLVNNLM 120
           SAKALAKE+VRS+KTVNRLHENKAQLNSISMHLGESVAIARTVGHLSKSAEVMKLVNNLM
Sbjct: 61  SAKALAKELVRSRKTVNRLHENKAQLNSISMHLGESVAIARTVGHLSKSAEVMKLVNNLM 120

Query: 121 KAPEMAKTMQEFSKEMTKAGVIEEFVNDAVDSALDSEDIEEEIEEEVDKVLTAIAGETVA 180
           KAPEMA TMQEFSKEMTKAGVIEE VNDAVDSALDSEDIEEEIEEEVDKVLTAIAGET A
Sbjct: 121 KAPEMAITMQEFSKEMTKAGVIEEIVNDAVDSALDSEDIEEEIEEEVDKVLTAIAGETAA 180

Query: 181 QLPEAVRKEKMKQPAHVQEVR-EEEAIAEGVDDEEELEEIRARLAKVRS 229
           QLPEAVRKE+MKQPAHVQEVR EEEAIAEGVDDEEELEEIRARLAKVRS
Sbjct: 181 QLPEAVRKERMKQPAHVQEVREEEEAIAEGVDDEEELEEIRARLAKVRS 229

BLAST of Chy12G214100 vs. ExPASy TrEMBL
Match: A0A6J1HYM6 (vacuolar protein sorting-associated protein 24 homolog 1 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111469281 PE=4 SV=1)

HSP 1 Score: 378.6 bits (971), Expect = 1.8e-101
Identity = 212/229 (92.58%), Postives = 222/229 (96.94%), Query Frame = 0

Query: 1   MEKVMNILKPKPNPQQQLRDWQRRLRQECRNVERQIRDIQREEKNVQKAIRDAAKRNDMV 60
           MEK+MN+LKPKPNPQQQLRDWQRRLRQECRN ERQIRDI+REEKNVQKAI+DAAKRNDMV
Sbjct: 1   MEKMMNVLKPKPNPQQQLRDWQRRLRQECRNFERQIRDIEREEKNVQKAIKDAAKRNDMV 60

Query: 61  SAKALAKEIVRSKKTVNRLHENKAQLNSISMHLGESVAIARTVGHLSKSAEVMKLVNNLM 120
           SAKALAKEIVRSKKTVNRLHENKAQ+NSISMHLGES+AIARTVGHLSKSAEVMKLVNNLM
Sbjct: 61  SAKALAKEIVRSKKTVNRLHENKAQVNSISMHLGESIAIARTVGHLSKSAEVMKLVNNLM 120

Query: 121 KAPEMAKTMQEFSKEMTKAGVIEEFVNDAVDSALDSEDIEEEIEEEVDKVLTAIAGETVA 180
           KAPEM  TM+EFSKEMTKAGVIEE VNDA+DSALDSEDIEEEIEEEVDKVLTAIAGET A
Sbjct: 121 KAPEMETTMREFSKEMTKAGVIEEIVNDALDSALDSEDIEEEIEEEVDKVLTAIAGETAA 180

Query: 181 QLPEAVRKEKMKQPAHVQEV-REEEAIAEGVDDEEELEEIRARLAKVRS 229
           QLPEAVRKE+MKQP+ VQEV REEEAIAEGVDDEEELEEIRARLAKVRS
Sbjct: 181 QLPEAVRKERMKQPSRVQEVRREEEAIAEGVDDEEELEEIRARLAKVRS 229

BLAST of Chy12G214100 vs. NCBI nr
Match: XP_004147294.1 (vacuolar protein sorting-associated protein 24 homolog 1 [Cucumis sativus] >KGN64811.1 hypothetical protein Csa_013890 [Cucumis sativus])

HSP 1 Score: 409 bits (1052), Expect = 2.51e-143
Identity = 226/228 (99.12%), Postives = 227/228 (99.56%), Query Frame = 0

Query: 1   MEKVMNILKPKPNPQQQLRDWQRRLRQECRNVERQIRDIQREEKNVQKAIRDAAKRNDMV 60
           MEKVMNILKPKPNPQQQLRDWQRRLRQECRNVERQIRDIQREEKNVQKAIRDAAKRNDMV
Sbjct: 1   MEKVMNILKPKPNPQQQLRDWQRRLRQECRNVERQIRDIQREEKNVQKAIRDAAKRNDMV 60

Query: 61  SAKALAKEIVRSKKTVNRLHENKAQLNSISMHLGESVAIARTVGHLSKSAEVMKLVNNLM 120
           SAKALAKEIVRSKKTVNRLHENKAQLNSISMHLGESVAIARTVGHLSKSAEVMKLVNNLM
Sbjct: 61  SAKALAKEIVRSKKTVNRLHENKAQLNSISMHLGESVAIARTVGHLSKSAEVMKLVNNLM 120

Query: 121 KAPEMAKTMQEFSKEMTKAGVIEEFVNDAVDSALDSEDIEEEIEEEVDKVLTAIAGETVA 180
           KAPEMAKTMQEFSKEMTKAGVIEEFVNDAVDSALDSEDIEEEIEEEVDKVLTAIAGET A
Sbjct: 121 KAPEMAKTMQEFSKEMTKAGVIEEFVNDAVDSALDSEDIEEEIEEEVDKVLTAIAGETAA 180

Query: 181 QLPEAVRKEKMKQPAHVQEVREEEAIAEGVDDEEELEEIRARLAKVRS 228
           QLPEAVRKEKMKQPAHVQEVREEEAIAEG+DDEEELEEIRARLAKVRS
Sbjct: 181 QLPEAVRKEKMKQPAHVQEVREEEAIAEGIDDEEELEEIRARLAKVRS 228

BLAST of Chy12G214100 vs. NCBI nr
Match: XP_008463000.1 (PREDICTED: vacuolar protein sorting-associated protein 24 homolog 1 [Cucumis melo] >KAA0051801.1 vacuolar protein sorting-associated protein 24-like protein 1 [Cucumis melo var. makuwa] >TYK21439.1 vacuolar protein sorting-associated protein 24-like protein 1 [Cucumis melo var. makuwa])

HSP 1 Score: 407 bits (1045), Expect = 2.93e-142
Identity = 225/228 (98.68%), Postives = 226/228 (99.12%), Query Frame = 0

Query: 1   MEKVMNILKPKPNPQQQLRDWQRRLRQECRNVERQIRDIQREEKNVQKAIRDAAKRNDMV 60
           MEK+MNILKPKPNPQQQLRDWQRRLRQECRNVERQIRDIQREEKNV KAIRDAAKRNDMV
Sbjct: 1   MEKMMNILKPKPNPQQQLRDWQRRLRQECRNVERQIRDIQREEKNVHKAIRDAAKRNDMV 60

Query: 61  SAKALAKEIVRSKKTVNRLHENKAQLNSISMHLGESVAIARTVGHLSKSAEVMKLVNNLM 120
           SAKALAKEIVRSKKTVNRLHENKAQLNSISMHLGESVAIARTVGHLSKSAEVMKLVNNLM
Sbjct: 61  SAKALAKEIVRSKKTVNRLHENKAQLNSISMHLGESVAIARTVGHLSKSAEVMKLVNNLM 120

Query: 121 KAPEMAKTMQEFSKEMTKAGVIEEFVNDAVDSALDSEDIEEEIEEEVDKVLTAIAGETVA 180
           KAPEMAKTMQEFSKEMTKAGVIEEFVNDAVDSALDSEDIEEEIEEEVDKVLTAIAGET A
Sbjct: 121 KAPEMAKTMQEFSKEMTKAGVIEEFVNDAVDSALDSEDIEEEIEEEVDKVLTAIAGETAA 180

Query: 181 QLPEAVRKEKMKQPAHVQEVREEEAIAEGVDDEEELEEIRARLAKVRS 228
           QLPEAVRKEKMKQPAHVQEVREEEAIAEGVDDEEELEEIRARLAKVRS
Sbjct: 181 QLPEAVRKEKMKQPAHVQEVREEEAIAEGVDDEEELEEIRARLAKVRS 228

BLAST of Chy12G214100 vs. NCBI nr
Match: XP_038894885.1 (vacuolar protein sorting-associated protein 24 homolog 1 [Benincasa hispida])

HSP 1 Score: 399 bits (1024), Expect = 4.81e-139
Identity = 223/229 (97.38%), Postives = 226/229 (98.69%), Query Frame = 0

Query: 1   MEKVMNILKPKPNPQQQLRDWQRRLRQECRNVERQIRDIQREEKNVQKAIRDAAKRNDMV 60
           MEK+M+ILKPKPNPQQQLRDWQRRLRQECRNVERQIRDIQREEKNVQKAIRDAAKRNDMV
Sbjct: 1   MEKMMSILKPKPNPQQQLRDWQRRLRQECRNVERQIRDIQREEKNVQKAIRDAAKRNDMV 60

Query: 61  SAKALAKEIVRSKKTVNRLHENKAQLNSISMHLGESVAIARTVGHLSKSAEVMKLVNNLM 120
           SAKALAKEIVRSKKTVNRLHENKAQLNSISMHLGESVAIARTVGHLSKSAEVMKLVNNLM
Sbjct: 61  SAKALAKEIVRSKKTVNRLHENKAQLNSISMHLGESVAIARTVGHLSKSAEVMKLVNNLM 120

Query: 121 KAPEMAKTMQEFSKEMTKAGVIEEFVNDAVDSALDSEDIEEEIEEEVDKVLTAIAGETVA 180
           KAPEMA TMQEFSKEMTKAGVIEEFVNDAVDSALDSEDIEEEIEEEVDKVLTAIAGET A
Sbjct: 121 KAPEMATTMQEFSKEMTKAGVIEEFVNDAVDSALDSEDIEEEIEEEVDKVLTAIAGETAA 180

Query: 181 QLPEAVRKEKMKQPAHVQEVREEE-AIAEGVDDEEELEEIRARLAKVRS 228
           QLPEAVRKE+MKQPAHVQEVREEE AIAEGVDDEEELEEIRARLAKVRS
Sbjct: 181 QLPEAVRKERMKQPAHVQEVREEEEAIAEGVDDEEELEEIRARLAKVRS 229

BLAST of Chy12G214100 vs. NCBI nr
Match: XP_022152871.1 (vacuolar protein sorting-associated protein 24 homolog 1 [Momordica charantia])

HSP 1 Score: 388 bits (996), Expect = 8.89e-135
Identity = 217/229 (94.76%), Postives = 224/229 (97.82%), Query Frame = 0

Query: 1   MEKVMNILKPKPNPQQQLRDWQRRLRQECRNVERQIRDIQREEKNVQKAIRDAAKRNDMV 60
           MEK+M++LKPK NPQQQLRDWQRRLRQECRNVERQIRDIQREEKNVQKAIR+AAKRNDMV
Sbjct: 1   MEKMMSMLKPKANPQQQLRDWQRRLRQECRNVERQIRDIQREEKNVQKAIREAAKRNDMV 60

Query: 61  SAKALAKEIVRSKKTVNRLHENKAQLNSISMHLGESVAIARTVGHLSKSAEVMKLVNNLM 120
           SAKALAKE+VRS+KTVNRLHENKAQLNSISMHLGESVAIARTVGHLSKSAEVMKLVNNLM
Sbjct: 61  SAKALAKELVRSRKTVNRLHENKAQLNSISMHLGESVAIARTVGHLSKSAEVMKLVNNLM 120

Query: 121 KAPEMAKTMQEFSKEMTKAGVIEEFVNDAVDSALDSEDIEEEIEEEVDKVLTAIAGETVA 180
           KAPEMA TMQEFSKEMTKAGVIEE VNDAVDSALDSEDIEEEIEEEVDKVLTAIAGET A
Sbjct: 121 KAPEMAITMQEFSKEMTKAGVIEEIVNDAVDSALDSEDIEEEIEEEVDKVLTAIAGETAA 180

Query: 181 QLPEAVRKEKMKQPAHVQEVREEE-AIAEGVDDEEELEEIRARLAKVRS 228
           QLPEAVRKE+MKQPAHVQEVREEE AIAEGVDDEEELEEIRARLAKVRS
Sbjct: 181 QLPEAVRKERMKQPAHVQEVREEEEAIAEGVDDEEELEEIRARLAKVRS 229

BLAST of Chy12G214100 vs. NCBI nr
Match: XP_022970267.1 (vacuolar protein sorting-associated protein 24 homolog 1 isoform X1 [Cucurbita maxima])

HSP 1 Score: 383 bits (984), Expect = 5.99e-133
Identity = 212/229 (92.58%), Postives = 222/229 (96.94%), Query Frame = 0

Query: 1   MEKVMNILKPKPNPQQQLRDWQRRLRQECRNVERQIRDIQREEKNVQKAIRDAAKRNDMV 60
           MEK+MN+LKPKPNPQQQLRDWQRRLRQECRN ERQIRDI+REEKNVQKAI+DAAKRNDMV
Sbjct: 1   MEKMMNVLKPKPNPQQQLRDWQRRLRQECRNFERQIRDIEREEKNVQKAIKDAAKRNDMV 60

Query: 61  SAKALAKEIVRSKKTVNRLHENKAQLNSISMHLGESVAIARTVGHLSKSAEVMKLVNNLM 120
           SAKALAKEIVRSKKTVNRLHENKAQ+NSISMHLGES+AIARTVGHLSKSAEVMKLVNNLM
Sbjct: 61  SAKALAKEIVRSKKTVNRLHENKAQVNSISMHLGESIAIARTVGHLSKSAEVMKLVNNLM 120

Query: 121 KAPEMAKTMQEFSKEMTKAGVIEEFVNDAVDSALDSEDIEEEIEEEVDKVLTAIAGETVA 180
           KAPEM  TM+EFSKEMTKAGVIEE VNDA+DSALDSEDIEEEIEEEVDKVLTAIAGET A
Sbjct: 121 KAPEMETTMREFSKEMTKAGVIEEIVNDALDSALDSEDIEEEIEEEVDKVLTAIAGETAA 180

Query: 181 QLPEAVRKEKMKQPAHVQEVR-EEEAIAEGVDDEEELEEIRARLAKVRS 228
           QLPEAVRKE+MKQP+ VQEVR EEEAIAEGVDDEEELEEIRARLAKVRS
Sbjct: 181 QLPEAVRKERMKQPSRVQEVRREEEAIAEGVDDEEELEEIRARLAKVRS 229

BLAST of Chy12G214100 vs. TAIR 10
Match: AT5G22950.1 (SNF7 family protein )

HSP 1 Score: 348.6 bits (893), Expect = 3.8e-96
Identity = 193/229 (84.28%), Postives = 217/229 (94.76%), Query Frame = 0

Query: 1   MEKVMNILKPKPNPQQQLRDWQRRLRQECRNVERQIRDIQREEKNVQKAIRDAAKRNDMV 60
           ME+VMNI+KPKP+P+Q LRDWQR+LRQECRN+ERQIRDIQ+EE+NVQKAI++AAKRNDMV
Sbjct: 1   MERVMNIIKPKPDPKQLLRDWQRKLRQECRNIERQIRDIQKEERNVQKAIKEAAKRNDMV 60

Query: 61  SAKALAKEIVRSKKTVNRLHENKAQLNSISMHLGESVAIARTVGHLSKSAEVMKLVNNLM 120
           SAKALAKEIV S++TVNRL+ENKAQ+NSISMHLGESVAIARTVGHLSKSAEVMKLVNNLM
Sbjct: 61  SAKALAKEIVSSRRTVNRLYENKAQMNSISMHLGESVAIARTVGHLSKSAEVMKLVNNLM 120

Query: 121 KAPEMAKTMQEFSKEMTKAGVIEEFVNDAVDSALDSEDIEEEIEEEVDKVLTAIAGETVA 180
           KAP+MA TMQEFSKEMTKAGVIEEFVN+A+D+ALDSED+EEEI+EEVDKVLTAIAGET A
Sbjct: 121 KAPQMAATMQEFSKEMTKAGVIEEFVNEAIDNALDSEDMEEEIDEEVDKVLTAIAGETAA 180

Query: 181 QLPEAVRKEKMKQPAH-VQEVREEEAIAEGVDDEEELEEIRARLAKVRS 229
           +LP AVRKE++K PA      REEEA+AEGVDDEEELEEIRARLAKVRS
Sbjct: 181 ELPVAVRKERIKVPAQKASTSREEEAVAEGVDDEEELEEIRARLAKVRS 229

BLAST of Chy12G214100 vs. TAIR 10
Match: AT3G45000.1 (SNF7 family protein )

HSP 1 Score: 252.7 bits (644), Expect = 2.9e-67
Identity = 147/198 (74.24%), Postives = 171/198 (86.36%), Query Frame = 0

Query: 32  VERQIRDIQREEKNVQKAIRDAAKRNDMVSAKALAKEIVRSKKTVNRLHENKAQLNSISM 91
           ++  + DI+REE+NV KAI+D+ KRND+V+AKALA+EIV S++TV RL+ENKAQ+NSISM
Sbjct: 3   IKSLLSDIEREERNVHKAIKDSVKRNDLVTAKALAREIVSSRRTVKRLYENKAQVNSISM 62

Query: 92  HLGESVAIARTVGHLSKSAEVMKLVNNLMKAPEMAKTMQEFSKEMTKAGVIEEFVNDAVD 151
           HLGES+A A TVG+LSKS EVMKLVN+LMKAPE+A TMQ FSKEMTK GVIEEFV+DAVD
Sbjct: 63  HLGESIATAVTVGNLSKSGEVMKLVNSLMKAPEIAVTMQAFSKEMTKTGVIEEFVSDAVD 122

Query: 152 SALDSEDIEEEIEEEVDKVLTAIAGETVAQLPEAVRKEKMKQPAHVQE-VREEEAIAEGV 211
           +ALDSEDIEEEIEEEVDKVLTAIAGET A+LPEAVRK+KM  PA   E +  E+AI EGV
Sbjct: 123 NALDSEDIEEEIEEEVDKVLTAIAGETAAELPEAVRKQKMNVPAQKAETLHGEDAIVEGV 182

Query: 212 DDEEELEEIRARLAKVRS 229
           D EEELE IR RLA VRS
Sbjct: 183 DAEEELEAIRYRLANVRS 200

BLAST of Chy12G214100 vs. TAIR 10
Match: AT3G45000.2 (SNF7 family protein )

HSP 1 Score: 184.1 bits (466), Expect = 1.3e-46
Identity = 109/139 (78.42%), Postives = 119/139 (85.61%), Query Frame = 0

Query: 91  MHLGESVAIARTVGHLSKSAEVMKLVNNLMKAPEMAKTMQEFSKEMTKAGVIEEFVNDAV 150
           MHLGES+A A TVG+LSKS EVMKLVN+LMKAPE+A TMQ FSKEMTK GVIEEFV+DAV
Sbjct: 1   MHLGESIATAVTVGNLSKSGEVMKLVNSLMKAPEIAVTMQAFSKEMTKTGVIEEFVSDAV 60

Query: 151 DSALDSEDIEEEIEEEVDKVLTAIAGETVAQLPEAVRKEKMKQPAHVQE-VREEEAIAEG 210
           D+ALDSEDIEEEIEEEVDKVLTAIAGET A+LPEAVRK+KM  PA   E +  E+AI EG
Sbjct: 61  DNALDSEDIEEEIEEEVDKVLTAIAGETAAELPEAVRKQKMNVPAQKAETLHGEDAIVEG 120

Query: 211 VDDEEELEEIRARLAKVRS 229
           VD EEELE IR RLA VRS
Sbjct: 121 VDAEEELEAIRYRLANVRS 139

BLAST of Chy12G214100 vs. TAIR 10
Match: AT1G03950.1 (vacuolar protein sorting-associated protein 2.3 )

HSP 1 Score: 81.6 bits (200), Expect = 8.8e-16
Identity = 62/223 (27.80%), Postives = 117/223 (52.47%), Query Frame = 0

Query: 5   MNILKPKPNPQQQLRDWQRRLRQECRNVERQIRDIQREEKNVQKAIRDAAKRNDMVSAKA 64
           MNI   KPNP++ LR+ +R + Q  R +E++I  +Q EEK +   I+  AK  +  + K 
Sbjct: 1   MNIFTKKPNPREVLRESKREMTQATRGIEKEIGSLQSEEKKLVLEIKRTAKSGNEGATKI 60

Query: 65  LAKEIVRSKKTVNRLHENKAQLNSISMHLGESVAIARTVGHLSKSAEVMKLVNNLMKAPE 124
           LA++++R ++ +  L  ++AQ+  I+ H     A       +  + + M  ++  M   +
Sbjct: 61  LARQLIRLRQQIANLQGSRAQMRGIATHTQAMHAHTSVAAGMQGATKAMAAMSKNMDPAK 120

Query: 125 MAKTMQEFSKEMTKAGVIEEFVNDAVDSALDSEDIEEEIEEEVDKVLTAIAGETVAQLPE 184
            AK M+EF K+  +  +  E ++D++D ALD+++ E+E E+  ++VL  I  +  +QL  
Sbjct: 121 QAKVMREFQKQSAQMDMTTEMMSDSIDDALDNDEAEDETEDLTNQVLDEIGIDIASQLSS 180

Query: 185 AVRKEKMKQPAHVQEVREEEAIAEGVDDEEELEEIRARLAKVR 228
           A +         +   + E+  + G+D      E+  RLA +R
Sbjct: 181 APK-------GKIGGKKAEDVGSSGID------ELEKRLAALR 210

BLAST of Chy12G214100 vs. TAIR 10
Match: AT5G44560.1 (SNF7 family protein )

HSP 1 Score: 73.6 bits (179), Expect = 2.4e-13
Identity = 60/223 (26.91%), Postives = 108/223 (48.43%), Query Frame = 0

Query: 5   MNILKPKPNPQQQLRDWQRRLRQECRNVERQIRDIQREEKNVQKAIRDAAKRNDMVSAKA 64
           MNI K K  P+  LR  +R +    R +ER+I  +Q EEK +   I+  AK  +  + K 
Sbjct: 1   MNIFKKKTTPKDALRTSKREMAVATRGIEREITSLQLEEKRLVAEIKKTAKTGNEAATKI 60

Query: 65  LAKEIVRSKKTVNRLHENKAQLNSISMHLGESVAIARTVGHLSKSAEVMKLVNNLMKAPE 124
           LA+++VR ++ +  L  ++AQ+  ++ H     A       +  + + M  +N  M   +
Sbjct: 61  LARQLVRLRQQITNLQGSRAQIRGVTTHTQALYASTSISSGMKGATKAMVAMNKQMAPTK 120

Query: 125 MAKTMQEFSKEMTKAGVIEEFVNDAVDSALDSEDIEEEIEEEVDKVLTAIAGETVAQLPE 184
            AK +++F K+  +  +  E +++A+D  LD ++ EEE E+  ++VL  I     +QL  
Sbjct: 121 QAKVIKDFQKQSAQLDMTIEMMSEAIDETLDKDEAEEETEDLTNQVLDEIGVGVASQLSS 180

Query: 185 AVRKEKMKQPAHVQEVREEEAIAEGVDDEEELEEIRARLAKVR 228
           A    K +            A      +  E++E+  RLA +R
Sbjct: 181 A---PKGRIATKTAAPPASTAATNKNSESSEVDELEKRLASLR 220

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FFB35.4e-9584.28Vacuolar protein sorting-associated protein 24 homolog 1 OS=Arabidopsis thaliana... [more]
Q9LXH54.0e-6674.24Putative vacuolar protein sorting-associated protein 24 homolog 2 OS=Arabidopsis... [more]
Q9CQ109.1e-3441.67Charged multivesicular body protein 3 OS=Mus musculus OX=10090 GN=Chmp3 PE=1 SV=... [more]
Q8CGS41.2e-3341.40Charged multivesicular body protein 3 OS=Rattus norvegicus OX=10116 GN=Chmp3 PE=... [more]
Q5RAU55.9e-3340.74Charged multivesicular body protein 3 OS=Pongo abelii OX=9601 GN=CHMP3 PE=2 SV=3[more]
Match NameE-valueIdentityDescription
A0A0A0LXU72.3e-10999.12Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G109340 PE=4 SV=1[more]
A0A5D3DDQ81.5e-10898.68Vacuolar protein sorting-associated protein 24-like protein 1 OS=Cucumis melo va... [more]
A0A1S3CIN51.5e-10898.68vacuolar protein sorting-associated protein 24 homolog 1 OS=Cucumis melo OX=3656... [more]
A0A6J1DJ207.3e-10394.76vacuolar protein sorting-associated protein 24 homolog 1 OS=Momordica charantia ... [more]
A0A6J1HYM61.8e-10192.58vacuolar protein sorting-associated protein 24 homolog 1 isoform X1 OS=Cucurbita... [more]
Match NameE-valueIdentityDescription
XP_004147294.12.51e-14399.12vacuolar protein sorting-associated protein 24 homolog 1 [Cucumis sativus] >KGN6... [more]
XP_008463000.12.93e-14298.68PREDICTED: vacuolar protein sorting-associated protein 24 homolog 1 [Cucumis mel... [more]
XP_038894885.14.81e-13997.38vacuolar protein sorting-associated protein 24 homolog 1 [Benincasa hispida][more]
XP_022152871.18.89e-13594.76vacuolar protein sorting-associated protein 24 homolog 1 [Momordica charantia][more]
XP_022970267.15.99e-13392.58vacuolar protein sorting-associated protein 24 homolog 1 isoform X1 [Cucurbita m... [more]
Match NameE-valueIdentityDescription
AT5G22950.13.8e-9684.28SNF7 family protein [more]
AT3G45000.12.9e-6774.24SNF7 family protein [more]
AT3G45000.21.3e-4678.42SNF7 family protein [more]
AT1G03950.18.8e-1627.80vacuolar protein sorting-associated protein 2.3 [more]
AT5G44560.12.4e-1326.91SNF7 family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 22..56
NoneNo IPR availableGENE3D6.10.140.1230coord: 9..185
e-value: 4.6E-59
score: 201.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 186..209
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 186..204
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..20
NoneNo IPR availablePANTHERPTHR10476CHARGED MULTIVESICULAR BODY PROTEINcoord: 3..228
NoneNo IPR availablePANTHERPTHR10476:SF39VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 24 HOMOLOG 1-LIKE ISOFORM X1coord: 3..228
IPR005024Snf7 familyPFAMPF03357Snf7coord: 21..188
e-value: 2.7E-29
score: 102.0

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy12G214100.1Chy12G214100.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032509 endosome transport via multivesicular body sorting pathway
biological_process GO:0045324 late endosome to vacuole transport
biological_process GO:0015031 protein transport
biological_process GO:0007034 vacuolar transport
cellular_component GO:0000815 ESCRT III complex
cellular_component GO:0005771 multivesicular body