Chy12G213900 (gene) Cucumber (hystrix) v1

Overview


Name	Chy12G213900
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	Cystatin Hv-CPI6
Location	chrH12: 12398793 .. 12399121 (-)
RNA-Seq Expression	Chy12G213900
Synteny	Chy12G213900

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TATTACTACATACACAATATGAGTAGAGCAGAACCAATTAAGGATGTCAATGACTCAGAAGTGAAATTTGCTGCAGGGGAAGCAGTTAAAATCCATAACGATAAAAAGCACGACAATTTGGTTTTCATTCGTGTAGTGAATGGTCTGAAGCAGCAAGTTGATAATGCCACGCTGTATTCTCTTCTGATAGAAGCTACAAATGGTGATGGAACTCATTGGGGATATTTCGCTAAGATCAAGTTGATACCTAAGCCCGCAAGTCATTATGATCCATATCCCCATAGCTTTCAGCTTATATCTTGGGAAGATCCCCTCAAATTCTACAACTA

mRNA sequence

TATTACTACATACACAATATGAGTAGAGCAGAACCAATTAAGGATGTCAATGACTCAGAAGTGAAATTTGCTGCAGGGGAAGCAGTTAAAATCCATAACGATAAAAAGCACGACAATTTGGTTTTCATTCGTGTAGTGAATGGTCTGAAGCAGCAAGTTGATAATGCCACGCTGTATTCTCTTCTGATAGAAGCTACAAATGGTGATGGAACTCATTGGGGATATTTCGCTAAGATCAAGTTGATACCTAAGCCCGCAAGTCATTATGATCCATATCCCCATAGCTTTCAGCTTATATCTTGGGAAGATCCCCTCAAATTCTACAACTA

Coding sequence (CDS)

TATTACTACATACACAATATGAGTAGAGCAGAACCAATTAAGGATGTCAATGACTCAGAAGTGAAATTTGCTGCAGGGGAAGCAGTTAAAATCCATAACGATAAAAAGCACGACAATTTGGTTTTCATTCGTGTAGTGAATGGTCTGAAGCAGCAAGTTGATAATGCCACGCTGTATTCTCTTCTGATAGAAGCTACAAATGGTGATGGAACTCATTGGGGATATTTCGCTAAGATCAAGTTGATACCTAAGCCCGCAAGTCATTATGATCCATATCCCCATAGCTTTCAGCTTATATCTTGGGAAGATCCCCTCAAATTCTACAACTA

Protein sequence

YYYIHNMSRAEPIKDVNDSEVKFAAGEAVKIHNDKKHDNLVFIRVVNGLKQQVDNATLYSLLIEATNGDGTHWGYFAKIKLIPKPASHYDPYPHSFQLISWEDPLKFYNX

Homology

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BLAST of Chy12G213900 vs. ExPASy Swiss-Prot
Match: Q84WT8 (Cysteine proteinase inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=CYS4 PE=3 SV=2)

HSP 1 Score: 46.6 bits (109), Expect = 2.1e-04
Identity = 27/69 (39.13%), Postives = 40/69 (57.97%), Query Frame = 0


Query: 11  EPIKDVNDSEVKFAAGEAVKIHNDKKHDNLVFIRVVNGLKQQVDNATLYSLLIEATNGDG 70
           +PIK+V+D +V   A  A++ HN +  + LVF++VV G  Q V + T Y L I A +G G
Sbjct: 33  KPIKNVSDPDVVAVAKYAIEEHNKESKEKLVFVKVVEGTTQVV-SGTKYDLKIAAKDGGG 92

Query: 71  THWGYFAKI 80
               Y A +
Sbjct: 93  KIKNYEAVV 100

[Close]

BLAST of Chy12G213900 vs. ExPASy TrEMBL
Match: A0A0A0LVH4 (Cystatin Hv-CPI6 OS=Cucumis sativus OX=3659 GN=Csa_1G123470 PE=4 SV=1)

HSP 1 Score: 188.3 bits (477), Expect = 1.7e-44
Identity = 88/103 (85.44%), Postives = 94/103 (91.26%), Query Frame = 0


Query: 7   MSRAEPIKDVNDSEVKFAAGEAVKIHNDKKHDNLVFIRVVNGLKQQVDNATLYSLLIEAT 66
           M RAEPIKDVNDSEVKFAAG+AVKIHND+KHD LVFIRVVNGLKQ+VD A LYSLLIEAT
Sbjct: 1   MGRAEPIKDVNDSEVKFAAGQAVKIHNDQKHDTLVFIRVVNGLKQEVDTAKLYSLLIEAT 60

Query: 67  NGDGTHWGYFAKIKLIPKPASHYDPYPHSFQLISWEDPLKFYN 110
           NGDGTHWGY AKIKL PK  SHYDPY H+F+LISWEDPLKF+N
Sbjct: 61  NGDGTHWGYIAKIKLWPKAPSHYDPYSHTFELISWEDPLKFWN 103

[Close]

BLAST of Chy12G213900 vs. ExPASy TrEMBL
Match: A0A5A7SX36 (Cystatin Hv-CPI6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold836G00250 PE=4 SV=1)

HSP 1 Score: 180.6 bits (457), Expect = 3.5e-42
Identity = 86/103 (83.50%), Postives = 92/103 (89.32%), Query Frame = 0


Query: 7   MSRAEPIKDVNDSEVKFAAGEAVKIHNDKKHDNLVFIRVVNGLKQQVDNATLYSLLIEAT 66
           MS AEPIKDVNDSEVKFAAGEAVKIHNDKK D+LVFIRVVNG+KQQVD    YSLLIEA 
Sbjct: 1   MSGAEPIKDVNDSEVKFAAGEAVKIHNDKKDDDLVFIRVVNGVKQQVDYGKQYSLLIEAR 60

Query: 67  NGDGTHWGYFAKIKLIPKPASHYDPYPHSFQLISWEDPLKFYN 110
           NGDGTHWGY AKIKL+ KP SH+DPYPHS+ LISWEDPLKFY+
Sbjct: 61  NGDGTHWGYCAKIKLLRKPVSHHDPYPHSYVLISWEDPLKFYD 103

[Close]

BLAST of Chy12G213900 vs. NCBI nr
Match: KGN64832.1 (hypothetical protein Csa_022861 [Cucumis sativus])

HSP 1 Score: 187 bits (475), Expect = 2.58e-59
Identity = 88/103 (85.44%), Postives = 94/103 (91.26%), Query Frame = 0


Query: 7   MSRAEPIKDVNDSEVKFAAGEAVKIHNDKKHDNLVFIRVVNGLKQQVDNATLYSLLIEAT 66
           M RAEPIKDVNDSEVKFAAG+AVKIHND+KHD LVFIRVVNGLKQ+VD A LYSLLIEAT
Sbjct: 1   MGRAEPIKDVNDSEVKFAAGQAVKIHNDQKHDTLVFIRVVNGLKQEVDTAKLYSLLIEAT 60

Query: 67  NGDGTHWGYFAKIKLIPKPASHYDPYPHSFQLISWEDPLKFYN 109
           NGDGTHWGY AKIKL PK  SHYDPY H+F+LISWEDPLKF+N
Sbjct: 61  NGDGTHWGYIAKIKLWPKAPSHYDPYSHTFELISWEDPLKFWN 103

[Close]

BLAST of Chy12G213900 vs. NCBI nr
Match: KAA0033827.1 (Cystatin Hv-CPI6 [Cucumis melo var. makuwa] >TYK21868.1 Cystatin Hv-CPI6 [Cucumis melo var. makuwa])

HSP 1 Score: 179 bits (455), Expect = 2.90e-56
Identity = 86/103 (83.50%), Postives = 92/103 (89.32%), Query Frame = 0


Query: 7   MSRAEPIKDVNDSEVKFAAGEAVKIHNDKKHDNLVFIRVVNGLKQQVDNATLYSLLIEAT 66
           MS AEPIKDVNDSEVKFAAGEAVKIHNDKK D+LVFIRVVNG+KQQVD    YSLLIEA 
Sbjct: 1   MSGAEPIKDVNDSEVKFAAGEAVKIHNDKKDDDLVFIRVVNGVKQQVDYGKQYSLLIEAR 60

Query: 67  NGDGTHWGYFAKIKLIPKPASHYDPYPHSFQLISWEDPLKFYN 109
           NGDGTHWGY AKIKL+ KP SH+DPYPHS+ LISWEDPLKFY+
Sbjct: 61  NGDGTHWGYCAKIKLLRKPVSHHDPYPHSYVLISWEDPLKFYD 103

[Close]

BLAST of Chy12G213900 vs. NCBI nr
Match: KAE8645934.1 (hypothetical protein Csa_021370 [Cucumis sativus])

HSP 1 Score: 62.4 bits (150), Expect = 6.45e-10
Identity = 38/105 (36.19%), Postives = 62/105 (59.05%), Query Frame = 0


Query: 9   RAEPIKDVND-SEVKFAAGEAVKIHNDKKHDNLVFIRVVNGLKQQVD--NATL--YSLLI 68
           +  PI +++   +V+   G AV IHN++K D L+F RV+NGL+  ++  N ++  Y+L++
Sbjct: 6   KGSPIPNIDQRKDVQQIGGIAVDIHNEEKSDTLIFHRVINGLELVINDINGSILSYTLVV 65

Query: 69  EATNGDGTHWGYFAKIKLIPKPASHYDPYPHSFQLISWEDPLKFY 108
           EA N D   W Y   IK I KP+S        ++L+ + DPL +Y
Sbjct: 66  EAINKDKNIWTYVTSIK-ITKPSS--------YELVYFRDPLGYY 101

[Close]

BLAST of Chy12G213900 vs. NCBI nr
Match: KAE8645932.1 (hypothetical protein Csa_021380 [Cucumis sativus])

HSP 1 Score: 62.0 bits (149), Expect = 9.11e-10
Identity = 38/107 (35.51%), Postives = 62/107 (57.94%), Query Frame = 0


Query: 8   SRAEPIKDVND-SEVKFAAGEAVKIHNDKKHDNLVFIRVVNGLKQQVDNA-----TLYSL 67
           ++  PI +++   +V+     AV IHN++K D L+F RV+NGL+  + +       LY+L
Sbjct: 5   NKGPPIPNIDQRKDVQQIGRIAVDIHNEQKPDTLIFHRVINGLEIGISDIHGPRILLYTL 64

Query: 68  LIEATNGDGTHWGYFAKIKLIPKPASHYDPYPHSFQLISWEDPLKFY 108
           ++EA N D   W Y   IK I KP+S        ++L+S++DPL +Y
Sbjct: 65  VVEAINKDKIIWTYVTSIK-ITKPSS--------YELVSFKDPLGYY 102

[Close]

BLAST of Chy12G213900 vs. NCBI nr
Match: KAE8647444.1 (hypothetical protein Csa_003412 [Cucumis sativus])

HSP 1 Score: 62.4 bits (150), Expect = 1.05e-09
Identity = 38/105 (36.19%), Postives = 62/105 (59.05%), Query Frame = 0


Query: 9   RAEPIKDVND-SEVKFAAGEAVKIHNDKKHDNLVFIRVVNGLKQQVD--NATL--YSLLI 68
           +  PI +++   +V+   G AV IHN++K D L+F RV+NGL+  ++  N ++  Y+L++
Sbjct: 6   KGSPIPNIDQRKDVQQIGGIAVDIHNEEKSDTLIFHRVINGLELVINDINGSILSYTLVV 65

Query: 69  EATNGDGTHWGYFAKIKLIPKPASHYDPYPHSFQLISWEDPLKFY 108
           EA N D   W Y   IK I KP+S        ++L+ + DPL +Y
Sbjct: 66  EAINKDKNIWTYVTSIK-ITKPSS--------YELVYFRDPLGYY 101

[Close]

BLAST of Chy12G213900 vs. TAIR 10
Match: AT4G16500.1 (Cystatin/monellin superfamily protein )

HSP 1 Score: 46.6 bits (109), Expect = 1.5e-05
Identity = 27/69 (39.13%), Postives = 40/69 (57.97%), Query Frame = 0


Query: 11  EPIKDVNDSEVKFAAGEAVKIHNDKKHDNLVFIRVVNGLKQQVDNATLYSLLIEATNGDG 70
           +PIK+V+D +V   A  A++ HN +  + LVF++VV G  Q V + T Y L I A +G G
Sbjct: 33  KPIKNVSDPDVVAVAKYAIEEHNKESKEKLVFVKVVEGTTQVV-SGTKYDLKIAAKDGGG 92

Query: 71  THWGYFAKI 80
               Y A +
Sbjct: 93  KIKNYEAVV 100

The following BLAST results are available for this feature:

BLAST of Chy12G213900 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q84WT8	2.1e-04	39.13	Cysteine proteinase inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=CYS4 PE=3 SV=...	[more]

BLAST of Chy12G213900 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LVH4	1.7e-44	85.44	Cystatin Hv-CPI6 OS=Cucumis sativus OX=3659 GN=Csa_1G123470 PE=4 SV=1	[more]
A0A5A7SX36	3.5e-42	83.50	Cystatin Hv-CPI6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold836G002...	[more]

BLAST of Chy12G213900 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN64832.1	2.58e-59	85.44	hypothetical protein Csa_022861 [Cucumis sativus]	[more]
KAA0033827.1	2.90e-56	83.50	Cystatin Hv-CPI6 [Cucumis melo var. makuwa] >TYK21868.1 Cystatin Hv-CPI6 [Cucumi...	[more]
KAE8645934.1	6.45e-10	36.19	hypothetical protein Csa_021370 [Cucumis sativus]	[more]
KAE8645932.1	9.11e-10	35.51	hypothetical protein Csa_021380 [Cucumis sativus]	[more]
KAE8647444.1	1.05e-09	36.19	hypothetical protein Csa_003412 [Cucumis sativus]	[more]

BLAST of Chy12G213900 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G16500.1	1.5e-05	39.13	Cystatin/monellin superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.10.450.10		coord: 7..103 e-value: 1.1E-13 score: 53.0
None	No IPR available	SUPERFAMILY	54403	Cystatin/monellin	coord: 11..72
IPR000010	Cystatin domain	PFAM	PF16845	SQAPI	coord: 13..78 e-value: 1.2E-12 score: 48.0

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy12G213900.1	Chy12G213900.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
molecular_function	GO:0004869	cysteine-type endopeptidase inhibitor activity