Chy12G213460 (gene) Cucumber (hystrix) v1

Overview
NameChy12G213460
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
Descriptionlysosomal Pro-X carboxypeptidase-like
LocationchrH12: 11438506 .. 11442678 (+)
RNA-Seq ExpressionChy12G213460
SyntenyChy12G213460
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTACAATGGATGGACAGTGCTGGCTTCATGGTTTAGGCTAAAGTATCCTCACATCGCTCTTGGAGCCCTGGCTTCTTCAGCTCCAATTCTTTACTTTGACAACATTACACCACAAGACGGATATTACTCGGTCGTCTCCAAATCTTTTAAAGTACGTCCCATCCATCCATATCCATTGAAACTTTAAACTAAAAAATGTGATATATATATGTGTGTTCTTTTGATCGATCACAAATATGATGATGCTTATATATAACAACATTTTTCATTGATCATGAAATGAAATTAGGAAGCGAGCAAAACGTGCCACGACACCATACGTAGATCGTGGGGTGAAATCGACCGAATTGCCGAGAAGACTCAGGGAGGGCTTTCGATTTTGAGCAAGCAATTCAAAACTTGTGGGAAATTGAAGACGAGTTCCGAGATCAAGAACTTAATGGACACTGTGTTTACAATGGCAGCTCAATACAACAATCCTTACGAGAATCCAGTGAGAGGCATATGTGTAGCCATTGATGAAGAAGCAAAGAAAAAAAGTAATGTGATCAAGCAAGTGGTTGCTGGGCTTATTGCTTATTTAGGGGAAAGGCCTTGTTATGATGTCTATGAATTTGGCTATCCTAATGATCCCCTTAACCAATATGGTTGGCAGGTACTTCTTTTTTCCTTTTATATTAAATTAAAATTTGGATACCCAATTTAAGCTCGACGGTTATTGACATAGTCTTTTTGTTTAGACACTGGAGTATTGATCTTCATAGTTGTGCTAAAAATTGATATGTTGGGCATAAAACTTAATCATGAATATTAATAACACGTTTTTTAGAATATACTGAACAGAAAAATTGAATCTCTCACTTCAATGTTAACAATATACAAGAACTTGTTATGAAAACCTACCACAAACCAAAAACATTTTAGTATATGAAAAAGGTTACGATCACAAAAAAATTTGTGCTCACCTACATACTCAATTATAAAATTACTCTCTTAAAACTTTTGACTACTCACACTCTCTCTCACTCTCAAACTAAATAATATAAAAGAAAATTTAACTAAAGTTAACACACCTAAGCTTAAGTGTTTCTAACTAGAGAATTTGAAATTAGAAGTACAAACTTCTTTCACAGTGCTTGGAGCCCATAGTATTTTTTGAAATTAAGGGCATCTAACACACGACAAAACCTGTGACACAAGATGTCACAACATTTCTAGAGAACATCATAGACAAATGCATAAAACTAAAGCCAATCATAGAGCACTGAGTTTGGTAACTCAGTCTAGTGAAACACACCACCTATGTGACTGTCTAGGAGCAATATGCTCTGGAAATATAGCTTCAGTAATATAAAGTCAAACAATTCCAACGATGTACTTATCTGTAAATAAACGAAAATTGCAATGACATTCGAATAGTGAAAATTAGAAAAGGATCAGTTACGCTCCCTCTAAATGTGAGATCCTTTCACCTTTAACGTACTAAGGCTCGTCCTTCTCACCAAGAACGTTTTCTCTTTCCACCTACAAATGAAATCCCCTTCACTTGAATAATATGACTCCTAGGTTTGAGAATCCCCCTCTCAAGTTGTTGAAGAATAAACCACCACATCTAGAGTAAATCTCAAGATCTACAACAATACAATCTTCTTCGACCAAAGATACACTAACACACTAGTGGTTTGAGAGCAAACACACAAGACAACTACTTCCAAGAATTTTCAAAACAACTCAACCTTAAAATTGAACAAAACAATGAACATATATATTGTATATATGTATACATATGCATAAAAAAGTTGAAATATAATTTGATCAATTTACTTTTCATCCGTTCATCCTATTCTAGTTTTTATACTTTTATAGTTCAGATTAGTCCTAATAAACTTTAAAAAATTTGTCCTTCCGAGTTATTTTTTTTTATTAAAATTGATTAAATTATTATTACTATTATTAATGCATGAAAATTCAATGTAAAAATATACTTCCAAGATTGCAATACTTATGGAAGTCTACTATTTGAAATTTGATTTGGTATGTCTATTTGTTTTCTTAAATTTTAGAATAGTTAGCTAATATAGATTGTGTCAAAATGAACCACATTTATATATTTGATTGTTATGTCTACAAGGTTTATAGATGTCCACAGCAAATATTGTCATAGAGTTACACTAGATTTTAAAATAAAGAAAACATCAATTTTACAAAGAACTAAATGCACACACATGCTATTAAAGTTCAATGATAATTTAATAATAAAAACAAGATGAAATACAAGTGACTGATAAATTAATGTAATTGTAGCATTAATAGTAATAAATATGTATTATGTATATATATCTATATTATATGTGTATTACAGTATAGTTATATTGTAAAGTACGTTGTTTCAGTTATATTCAATAATTTGTTTTCACGTATAGGTTTGTAGCGAGATGGTAATGCCCATTGGCATTAGTGGTAGGGACAAAAACTCAATGTTCCCACCTTCACCCTTCCAGTTCAACGACTTCAAAACTATGTGCAAGGATTTGTATGGTGTCACACCAAGGCCTCATTGGATCACCACTTTCTATGGAGGCCAAGTAAGCTCTCTTTAACCTTTCATACTAAACAAAAAATTTCTACACAATAAACACCAAATTCTTTTTCCCTTCTTTAATCTAAGAAAATATCATAATATTGAAAAGATACGTGTCACTAACAATTTTTGGAAAAAAAGATAGTGTCATTTATTGGTGAACTTAAGCTCACTTTAATCTCATTTCTTAAACCTAACTAACTTGACAGAAAAAATATATAAAGCGATCAAAATCCTCTTGCATTAATTTTTCTTATTGGTAATGGGTTATTTGTCTCTTTCTTTAATCTAATTAGAGATAGATAGTGTATTAGAATTTTTTTTTGAAAAAGTAACTTTTAAATATAAAAGCATTTTTTTCTAGATTACTGTAAAGGTTGAAATAGTTTAAATAAAATATAACAAAATTTTAGATTTCATCGATTATCAATATCTATCGGTGACATTGATAAACACTAATAGAATGTTTATTATTGGTAGAATAAATTTTTTTATTATATTTTGTAAATATTTTGATTTATTTTATTATATTTAAAAATATTCTTTTTTCAATAAGAACATTTTAAAAATTATTAATTGAAAGTGTGTTTAATATAATTTTTTTAATGGTTTATTAGGAACACAAATGATTTTTAATTCTAATAAAATAAAATGTCATCCTAGAAAAAAGAAAATTGACAAAGTATTTATACTTCATAGAAAAATTAAGTGTAATAAATATTGTCTTCTAGTGTTTTTTTTTCTATGGAGTATAAATAATTTGTCAATTTTTCTATTTTTGGAATACCCCAATTATAAACTTAGATTAGTTTAAAAAGTTATATTTTCTTTAAGAAAAGCTTGAAAGGTTAATCCTCAAAACATGATTTTTTAAAGGGATAGTTGCAAATTTAGCAATTAGATTTAATTATATTAAATATATAGAAACATTTTTAAAAAATTACAAATACAACAAAATTTGTCAAATTCTACAAAGGATAGAAATCTATCACTGATAGACCATACAAGTCTACCAACGATACAATTCTATCAGTGATAGTTTTGCTATATTTACAATTCTTTTTAAATATCCTTAATAATTATTCTTAAAATCGTGTCAATTATAATTATCTTTTTTTTAAAATTATTAGTTAACTAGAAACTTCTAAACATATAATGGCATTTTAAGAAAATTATTTTTACTTCGAATTTCATTTAATTTTATTCTACATATTCTTAATAAATCTTAATGAATATTAAATAAAGAGAAAAAAACAAACGCATGATCCAAGATCTTTCTAAGACTCATGTTCTAACATCATCTTAATTCACTTATTGACAAAAAAAGAAAGAAAGTTAAAGTTGACGTGTGAAACAAATTTAAAATAATATATCTAACATTTATTACTTTAAACTTAAATTTTAATTAAAATTGGTTAAATGATGATAATAATTTTCATCCAAATAAAATACACTATATATATATATATAATATTATTATTTAAAGATTTTTTTTTTCTTTTGTAGGACATAAAATTAGTGCTGCATCGGTTTGGAAGTAACATCATTTTCTCTAATGGACTCAAAGATCCTTACAGCAGTGGCGGGTAA

mRNA sequence

ATGTACAATGGATGGACAGTGCTGGCTTCATGGTTTAGGCTAAAGTATCCTCACATCGCTCTTGGAGCCCTGGCTTCTTCAGCTCCAATTCTTTACTTTGACAACATTACACCACAAGACGGATATTACTCGGTCGTCTCCAAATCTTTTAAAGAAGCGAGCAAAACGTGCCACGACACCATACGTAGATCGTGGGGTGAAATCGACCGAATTGCCGAGAAGACTCAGGGAGGGCTTTCGATTTTGAGCAAGCAATTCAAAACTTGTGGGAAATTGAAGACGAGTTCCGAGATCAAGAACTTAATGGACACTGTGTTTACAATGGCAGCTCAATACAACAATCCTTACGAGAATCCAGTGAGAGGCATATGTGTAGCCATTGATGAAGAAGCAAAGAAAAAAAGTAATGTGATCAAGCAAGTGGTTGCTGGGCTTATTGCTTATTTAGGGGAAAGGCCTTGTTATGATGTCTATGAATTTGGCTATCCTAATGATCCCCTTAACCAATATGGTTGGCAGGTTTGTAGCGAGATGGTAATGCCCATTGGCATTAGTGGTAGGGACAAAAACTCAATGTTCCCACCTTCACCCTTCCAGTTCAACGACTTCAAAACTATGTGCAAGGATTTGTATGGTGTCACACCAAGGCCTCATTGGATCACCACTTTCTATGGAGGCCAAGACATAAAATTAGTGCTGCATCGGTTTGGAAGTAACATCATTTTCTCTAATGGACTCAAAGATCCTTACAGCAGTGGCGGGTAA

Coding sequence (CDS)

ATGTACAATGGATGGACAGTGCTGGCTTCATGGTTTAGGCTAAAGTATCCTCACATCGCTCTTGGAGCCCTGGCTTCTTCAGCTCCAATTCTTTACTTTGACAACATTACACCACAAGACGGATATTACTCGGTCGTCTCCAAATCTTTTAAAGAAGCGAGCAAAACGTGCCACGACACCATACGTAGATCGTGGGGTGAAATCGACCGAATTGCCGAGAAGACTCAGGGAGGGCTTTCGATTTTGAGCAAGCAATTCAAAACTTGTGGGAAATTGAAGACGAGTTCCGAGATCAAGAACTTAATGGACACTGTGTTTACAATGGCAGCTCAATACAACAATCCTTACGAGAATCCAGTGAGAGGCATATGTGTAGCCATTGATGAAGAAGCAAAGAAAAAAAGTAATGTGATCAAGCAAGTGGTTGCTGGGCTTATTGCTTATTTAGGGGAAAGGCCTTGTTATGATGTCTATGAATTTGGCTATCCTAATGATCCCCTTAACCAATATGGTTGGCAGGTTTGTAGCGAGATGGTAATGCCCATTGGCATTAGTGGTAGGGACAAAAACTCAATGTTCCCACCTTCACCCTTCCAGTTCAACGACTTCAAAACTATGTGCAAGGATTTGTATGGTGTCACACCAAGGCCTCATTGGATCACCACTTTCTATGGAGGCCAAGACATAAAATTAGTGCTGCATCGGTTTGGAAGTAACATCATTTTCTCTAATGGACTCAAAGATCCTTACAGCAGTGGCGGGTAA

Protein sequence

MYNGWTVLASWFRLKYPHIALGALASSAPILYFDNITPQDGYYSVVSKSFKEASKTCHDTIRRSWGEIDRIAEKTQGGLSILSKQFKTCGKLKTSSEIKNLMDTVFTMAAQYNNPYENPVRGICVAIDEEAKKKSNVIKQVVAGLIAYLGERPCYDVYEFGYPNDPLNQYGWQVCSEMVMPIGISGRDKNSMFPPSPFQFNDFKTMCKDLYGVTPRPHWITTFYGGQDIKLVLHRFGSNIIFSNGLKDPYSSGG*
Homology
BLAST of Chy12G213460 vs. ExPASy Swiss-Prot
Match: Q2TA14 (Lysosomal Pro-X carboxypeptidase OS=Bos taurus OX=9913 GN=PRCP PE=2 SV=1)

HSP 1 Score: 161.4 bits (407), Expect = 1.4e-38
Identity = 92/263 (34.98%), Postives = 140/263 (53.23%), Query Frame = 0

Query: 7   VLASWFRLKYPHIALGALASSAPILYFDNITPQDGYYSVVSKSFKEASKTCHDTIRRSWG 66
           +LA+WFR+KYPH+ +GALASSAPI  F+++ P D +  +V+  F ++   C ++IRRSW 
Sbjct: 185 MLAAWFRMKYPHLVVGALASSAPIWQFNDLVPCDIFMKIVTTDFSQSGPNCSESIRRSWD 244

Query: 67  EIDRIAEKTQGGLSILSKQFKTCGKLKTSSEIKNLMDTV---FTMAAQYNNPYEN----- 126
            I+R+A+K   GL  LS+    C  L  S +++ L D +   +   A  + PYE+     
Sbjct: 245 AINRLAKKGT-GLRWLSEALHLCTPLTKSQDVQRLKDWISETWVNVAMVDYPYESNFLQP 304

Query: 127 ----PVRGICVAIDEEAKKKSNVIKQVVAGLIAYL---GERPCYDVYEFGYPNDPLNQYG 186
               PV+ +C          + +++ +   L  Y    G+  C +V E    +  +  + 
Sbjct: 305 LPAWPVKVVCQYFKYSNVPDTVMVQNIFQALNVYYNYSGQAKCLNVSETATSSLGVLGWS 364

Query: 187 WQVCSEMVMPIGISGRDKNSMFPPSPFQFNDFKTMCKDLYGVTPRPHWITTFYGGQDIKL 246
           +Q C+EMVMP    G D   MF P  +   ++   C   +GV PRP WI T YGG++I  
Sbjct: 365 YQACTEMVMPTCSDGVD--DMFEPHSWNMKEYSDDCFKQWGVRPRPSWIPTMYGGKNIS- 424

Query: 247 VLHRFGSNIIFSNGLKDPYSSGG 255
                 +NIIFSNG  DP+S GG
Sbjct: 425 ----SHTNIIFSNGELDPWSGGG 439

BLAST of Chy12G213460 vs. ExPASy Swiss-Prot
Match: Q5RBU7 (Lysosomal Pro-X carboxypeptidase OS=Pongo abelii OX=9601 GN=PRCP PE=2 SV=1)

HSP 1 Score: 153.3 bits (386), Expect = 3.7e-36
Identity = 90/265 (33.96%), Postives = 140/265 (52.83%), Query Frame = 0

Query: 7   VLASWFRLKYPHIALGALASSAPILYFDNITPQDGYYSVVSKSFKEASKTCHDTIRRSWG 66
           +LA+WFR+KYPH+ +GALA+SAPI  F+++ P   +  +V+  F+++   C ++IRRSW 
Sbjct: 183 MLAAWFRMKYPHMVVGALAASAPIWQFEDLVPCGVFMKIVTTDFRKSGPHCSESIRRSWD 242

Query: 67  EIDRIAEKTQGGLSILSKQFKTCGKLKTSSEIKNLMDTV---FTMAAQYNNPYEN----- 126
            I+R++  T  GL  L+     C  L TS +I++L D +   +   A  + PY +     
Sbjct: 243 AINRLS-NTGSGLQWLTGALHLCSPL-TSQDIQHLKDWISETWVNLAMVDYPYASNFLQP 302

Query: 127 ----PVRGICVAIDEEAKKKSNVIKQVVAGLIAYL---GERPCYDVYEFGYPNDPLNQYG 186
               P++ +C  +       S +++ +   L  Y    G+  C ++ E       L   G
Sbjct: 303 LPAWPIKVVCQYLKNPNVSDSLLLQNIFQALNVYYNYSGQVKCLNISETA--TSSLGTLG 362

Query: 187 W--QVCSEMVMPIGISGRDKNSMFPPSPFQFNDFKTMCKDLYGVTPRPHWITTFYGGQDI 246
           W  Q C+E+VMP   +G D   MF P  +   +    C   +GV PRP WITT YGG++I
Sbjct: 363 WSYQACTEVVMPFCTNGVD--DMFEPHSWNLKELSDDCFQQWGVRPRPSWITTMYGGKNI 422

Query: 247 KLVLHRFGSNIIFSNGLKDPYSSGG 255
                   +NI+FSNG  DP+S GG
Sbjct: 423 S-----SHTNIVFSNGELDPWSGGG 436

BLAST of Chy12G213460 vs. ExPASy Swiss-Prot
Match: P42785 (Lysosomal Pro-X carboxypeptidase OS=Homo sapiens OX=9606 GN=PRCP PE=1 SV=1)

HSP 1 Score: 151.4 bits (381), Expect = 1.4e-35
Identity = 89/265 (33.58%), Postives = 139/265 (52.45%), Query Frame = 0

Query: 7   VLASWFRLKYPHIALGALASSAPILYFDNITPQDGYYSVVSKSFKEASKTCHDTIRRSWG 66
           +LA+WFR+KYPH+ +GALA+SAPI  F+++ P   +  +V+  F+++   C ++I RSW 
Sbjct: 183 MLAAWFRMKYPHMVVGALAASAPIWQFEDLVPCGVFMKIVTTDFRKSGPHCSESIHRSWD 242

Query: 67  EIDRIAEKTQGGLSILSKQFKTCGKLKTSSEIKNLMDTV---FTMAAQYNNPYEN----- 126
            I+R++  T  GL  L+     C  L TS +I++L D +   +   A  + PY +     
Sbjct: 243 AINRLS-NTGSGLQWLTGALHLCSPL-TSQDIQHLKDWISETWVNLAMVDYPYASNFLQP 302

Query: 127 ----PVRGICVAIDEEAKKKSNVIKQVVAGLIAYL---GERPCYDVYEFGYPNDPLNQYG 186
               P++ +C  +       S +++ +   L  Y    G+  C ++ E       L   G
Sbjct: 303 LPAWPIKVVCQYLKNPNVSDSLLLQNIFQALNVYYNYSGQVKCLNISETA--TSSLGTLG 362

Query: 187 W--QVCSEMVMPIGISGRDKNSMFPPSPFQFNDFKTMCKDLYGVTPRPHWITTFYGGQDI 246
           W  Q C+E+VMP   +G D   MF P  +   +    C   +GV PRP WITT YGG++I
Sbjct: 363 WSYQACTEVVMPFCTNGVD--DMFEPHSWNLKELSDDCFQQWGVRPRPSWITTMYGGKNI 422

Query: 247 KLVLHRFGSNIIFSNGLKDPYSSGG 255
                   +NI+FSNG  DP+S GG
Sbjct: 423 S-----SHTNIVFSNGELDPWSGGG 436

BLAST of Chy12G213460 vs. ExPASy Swiss-Prot
Match: Q7TMR0 (Lysosomal Pro-X carboxypeptidase OS=Mus musculus OX=10090 GN=Prcp PE=1 SV=2)

HSP 1 Score: 151.4 bits (381), Expect = 1.4e-35
Identity = 90/265 (33.96%), Postives = 136/265 (51.32%), Query Frame = 0

Query: 7   VLASWFRLKYPHIALGALASSAPILYFDNITPQDGYYSVVSKSFKEASKTCHDTIRRSWG 66
           +LA+WFR+KYPHI +GALA+SAPI   D + P   +  +V+  F+++   C ++IR+SW 
Sbjct: 181 MLAAWFRMKYPHIVVGALAASAPIWQLDGMVPCGEFMKIVTNDFRKSGPYCSESIRKSWN 240

Query: 67  EIDRIAEKTQGGLSILSKQFKTCGKLKTSSEIKNLMDTV---FTMAAQYNNPYEN----- 126
            ID+++  +  GL  L+     C  L TS +I  L   +   +   A  N PY       
Sbjct: 241 VIDKLS-GSGSGLQSLTNILHLCSPL-TSEKIPTLKGWIAETWVNLAMVNYPYACNFLQP 300

Query: 127 ----PVRGICVAIDEEAKKKSNVIKQVVAGLIAYL---GERPCYDVYEFGYPNDPLNQYG 186
               P++ +C  +       + +++ +   L  Y    G+  C ++ +       L   G
Sbjct: 301 LPAWPIKEVCQYLKNPNVSDTVLLQNIFQALSVYYNYSGQAACLNISQ--TTTSSLGSMG 360

Query: 187 W--QVCSEMVMPIGISGRDKNSMFPPSPFQFNDFKTMCKDLYGVTPRPHWITTFYGGQDI 246
           W  Q C+EMVMP   +G D   MF P  +    +   C + +GV PRPHW+TT YGG++I
Sbjct: 361 WSFQACTEMVMPFCTNGID--DMFEPFLWDLEKYSNDCFNQWGVKPRPHWMTTMYGGKNI 420

Query: 247 KLVLHRFGSNIIFSNGLKDPYSSGG 255
                   SNIIFSNG  DP+S GG
Sbjct: 421 S-----SHSNIIFSNGELDPWSGGG 434

BLAST of Chy12G213460 vs. ExPASy Swiss-Prot
Match: Q9EPB1 (Dipeptidyl peptidase 2 OS=Rattus norvegicus OX=10116 GN=Dpp7 PE=1 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 4.1e-27
Identity = 82/270 (30.37%), Postives = 130/270 (48.15%), Query Frame = 0

Query: 7   VLASWFRLKYPHIALGALASSAPILYFDNITPQDGYYSVVSKSFKEASKTCHDTIRRSWG 66
           +L+++ R+KYPH+  GALA+SAP++    +   D ++  V+  F   S  C   +R ++ 
Sbjct: 176 MLSAYMRMKYPHLVAGALAASAPVIAVAGLGNPDQFFRDVTADFYGQSPKCAQAVRDAFQ 235

Query: 67  EIDRIAEKTQGGLSILSKQFKTCGKLKTSSEIKNLMD---TVFTMAAQYNNPY------- 126
           +I  +    QG    +S+ F TC  L +  ++  L       FT+ A  + PY       
Sbjct: 236 QIKDLF--LQGAYDTISQNFGTCQSLSSPKDLTQLFGFARNAFTVLAMMDYPYPTNFLGP 295

Query: 127 --ENPVRGICVAIDEEAKKKSNVIKQVVAGLI-AYLGERPCYDVYE-FGYPNDPL----- 186
              NPV+  C  +  E ++   +  + +AGL+    G  PC+D+Y+ +    DP      
Sbjct: 296 LPANPVKVGCERLLSEGQRIMGL--RALAGLVYNSSGMEPCFDIYQMYQSCADPTGCGTG 355

Query: 187 -NQYGW--QVCSEMVMPIGISGRDKNSMFPPSPFQFNDFKTMCKDLYGVTPRPHWITTFY 246
            N   W  Q C+E  + +     +   MFP  PF     +  C D +GV PRP W+ T +
Sbjct: 356 SNARAWDYQACTE--INLTFDSNNVTDMFPEIPFSDELRQQYCLDTWGVWPRPDWLQTSF 415

Query: 247 GGQDIKLVLHRFGSNIIFSNGLKDPYSSGG 255
            G D+K       SNIIFSNG  DP++ GG
Sbjct: 416 WGGDLKA-----ASNIIFSNGDLDPWAGGG 434

BLAST of Chy12G213460 vs. ExPASy TrEMBL
Match: A0A6J1FJ87 (lysosomal Pro-X carboxypeptidase-like OS=Cucurbita moschata OX=3662 GN=LOC111444394 PE=3 SV=1)

HSP 1 Score: 400.6 bits (1028), Expect = 4.9e-108
Identity = 183/248 (73.79%), Postives = 214/248 (86.29%), Query Frame = 0

Query: 7   VLASWFRLKYPHIALGALASSAPILYFDNITPQDGYYSVVSKSFKEASKTCHDTIRRSWG 66
           +LASWFRLKYPHIALGALASSAPILYFDNITPQDGYYS+VSKSFKE S+TC++TIRRSW 
Sbjct: 189 MLASWFRLKYPHIALGALASSAPILYFDNITPQDGYYSIVSKSFKETSQTCYETIRRSWA 248

Query: 67  EIDRIAEKTQGGLSILSKQFKTCGKLKTSSEIKNLMDTVFTMAAQYNNPYENPVRGICVA 126
           E+DRIA     GL ILSK+FKTC KL  S E+K  +D V T AAQYNNP+E PVRGIC A
Sbjct: 249 EVDRIANNKPEGLMILSKRFKTCEKLNGSDELKYYLDHVLTSAAQYNNPHEKPVRGICAA 308

Query: 127 IDEEAKKKSNVIKQVVAGLIAYLGERPCYDVYEFGYPNDPLNQYGWQVCSEMVMPIGISG 186
           IDE A+K S+VI+QVVAG++A++GER CYDV+E G+P++P++ + WQ  SE+VMPIGISG
Sbjct: 309 IDEAARKNSDVIEQVVAGVVAFMGERDCYDVFESGHPDNPIDPFTWQEYSEIVMPIGISG 368

Query: 187 RDKNSMFPPSPFQFNDFKTMCKDLYGVTPRPHWITTFYGGQDIKLVLHRFGSNIIFSNGL 246
           +DK+SMFP +PF FN FK  CK LYGV+P PHWITTFYGGQD+KL+LHRFGSNIIFSNGL
Sbjct: 369 KDKDSMFPTAPFDFNKFKKDCKALYGVSPNPHWITTFYGGQDLKLILHRFGSNIIFSNGL 428

Query: 247 KDPYSSGG 255
           KDPYSSGG
Sbjct: 429 KDPYSSGG 436

BLAST of Chy12G213460 vs. ExPASy TrEMBL
Match: A0A6J1FDJ0 (lysosomal Pro-X carboxypeptidase-like OS=Cucurbita moschata OX=3662 GN=LOC111444393 PE=3 SV=1)

HSP 1 Score: 393.3 bits (1009), Expect = 7.9e-106
Identity = 184/248 (74.19%), Postives = 213/248 (85.89%), Query Frame = 0

Query: 7   VLASWFRLKYPHIALGALASSAPILYFDNITPQDGYYSVVSKSFKEASKTCHDTIRRSWG 66
           +LASWFRLKYPHIALGALASSAPILYFDNITPQDGYYS+VSKSFKE S+TC++TIRRSW 
Sbjct: 188 MLASWFRLKYPHIALGALASSAPILYFDNITPQDGYYSIVSKSFKETSQTCYETIRRSWA 247

Query: 67  EIDRIAEKTQGGLSILSKQFKTCGKLKTSSEIKNLMDTVFTMAAQYNNPYENPVRGICVA 126
           EIDRIA+    GL ILSK+FKTC KL  S E+KN +D VFT AAQYN P+E PVRGIC A
Sbjct: 248 EIDRIAKNKPEGLMILSKRFKTCKKLNESHELKNHLDNVFTYAAQYNEPHEKPVRGICAA 307

Query: 127 IDEEAKKKSNVIKQVVAGLIAYLGERPCYDVYEFGYPNDPLNQYGWQVCSEMVMPIGISG 186
           IDE A+K S+VI+QVVAG++A +GER CYDV+EFG P++ ++ + WQV SEMVMPIGISG
Sbjct: 308 IDEAARKNSDVIEQVVAGVVALMGERDCYDVFEFGDPDNTIDPFTWQVYSEMVMPIGISG 367

Query: 187 RDKNSMFPPSPFQFNDFKTMCKDLYGVTPRPHWITTFYGGQDIKLVLHRFGSNIIFSNGL 246
           ++ +SMFP +PF FN FK  CK LYGV+P  HWITTFYGGQD+KL+LHRFGSNIIFSNGL
Sbjct: 368 KE-DSMFPTAPFDFNKFKEDCKALYGVSPNRHWITTFYGGQDLKLILHRFGSNIIFSNGL 427

Query: 247 KDPYSSGG 255
           KDPYSSGG
Sbjct: 428 KDPYSSGG 434

BLAST of Chy12G213460 vs. ExPASy TrEMBL
Match: A0A6J1DJ73 (lysosomal Pro-X carboxypeptidase-like OS=Momordica charantia OX=3673 GN=LOC111020537 PE=3 SV=1)

HSP 1 Score: 372.1 bits (954), Expect = 1.9e-99
Identity = 181/249 (72.69%), Postives = 206/249 (82.73%), Query Frame = 0

Query: 7   VLASWFRLKYPHIALGALASSAPILYFDNITPQDGYYSVVSKSFKEASKTCHDTIRRSWG 66
           +LASWFRLKYPHIALGALASSAPILYFDNITPQDGYYSVVSKSF+E S+TC++ IRRSW 
Sbjct: 189 MLASWFRLKYPHIALGALASSAPILYFDNITPQDGYYSVVSKSFRETSETCYEIIRRSWA 248

Query: 67  EIDRIAEKTQGGLSILSKQFKTCGKLKTSSEIKNLMDTVFTMAAQYNNPYENPVRGICVA 126
           EIDRIAE+   GLSILSK+FKTCGKL  SSE+K+ +D+V T AAQYN P  NPV  IC A
Sbjct: 249 EIDRIAEEKAQGLSILSKRFKTCGKLNRSSELKDYLDSVLTEAAQYNFPSANPVDAICAA 308

Query: 127 IDEEAKKK-SNVIKQVVAGLIAYLGERPCYDVYEFGYPNDPLNQYGWQVCSEMVMPIGIS 186
           ID EAKKK S++I QV AG++AY+GER CYDV +  Y +D  +QY WQVCSEMVMPIG S
Sbjct: 309 IDGEAKKKSSDLIGQVFAGVVAYMGERSCYDVSD--YDHDSTDQYSWQVCSEMVMPIGRS 368

Query: 187 GRDKNSMFPPSPFQFNDFKTMCKDLYGVTPRPHWITTFYGGQDIKLVLHRFGSNIIFSNG 246
           G+  +SMFP + F  N FK  CK  YGV+P+PHWITTFYGG D+KLVLHRF SNIIFSNG
Sbjct: 369 GKG-DSMFPTAQFDLNSFKNDCKAWYGVSPKPHWITTFYGGHDLKLVLHRFASNIIFSNG 428

Query: 247 LKDPYSSGG 255
           LKDPYSSGG
Sbjct: 429 LKDPYSSGG 434

BLAST of Chy12G213460 vs. ExPASy TrEMBL
Match: A0A6J1DHH2 (lysosomal Pro-X carboxypeptidase-like OS=Momordica charantia OX=3673 GN=LOC111020528 PE=3 SV=1)

HSP 1 Score: 370.2 bits (949), Expect = 7.1e-99
Identity = 177/249 (71.08%), Postives = 208/249 (83.53%), Query Frame = 0

Query: 7   VLASWFRLKYPHIALGALASSAPILYFDNITPQDGYYSVVSKSFKEASKTCHDTIRRSWG 66
           +LASWFRLKYPHIALGALASSAP+LYFDNITPQDGYYSVVSKSF+E S+TC++ IRRSW 
Sbjct: 209 MLASWFRLKYPHIALGALASSAPVLYFDNITPQDGYYSVVSKSFRETSETCYEIIRRSWA 268

Query: 67  EIDRIAEKTQGGLSILSKQFKTCGKLKTSSEIKNLMDTVFTMAAQYNNPYENPVRGICVA 126
           EIDRIAEK Q GLSILSK+FKTC KL  SS++K+ +D +F+ AAQYN P ENPV  IC A
Sbjct: 269 EIDRIAEKPQ-GLSILSKRFKTCAKLNRSSDLKDYLDNMFSGAAQYNFPSENPVDAICAA 328

Query: 127 IDEEAKKK-SNVIKQVVAGLIAYLGERPCYDVYEFGYPNDPLNQYGWQVCSEMVMPIGIS 186
           ID EAKKK S++I QV AG++AY+GE+PCYDV +  +  DP +QY +Q+CSEMV+PIG+S
Sbjct: 329 IDGEAKKKTSDLIGQVFAGVVAYMGEKPCYDVSDSWHTVDPTDQYSFQICSEMVIPIGVS 388

Query: 187 GRDKNSMFPPSPFQFNDFKTMCKDLYGVTPRPHWITTFYGGQDIKLVLHRFGSNIIFSNG 246
           G+   SMFP +PF  N FK  CK  YGV+P+PHWITTFYGG D+KLVL RF SNIIFSNG
Sbjct: 389 GK-VESMFPTAPFDLNSFKNDCKAWYGVSPKPHWITTFYGGHDLKLVLQRFASNIIFSNG 448

Query: 247 LKDPYSSGG 255
           LKDPYSSGG
Sbjct: 449 LKDPYSSGG 455

BLAST of Chy12G213460 vs. ExPASy TrEMBL
Match: A0A0A0LV80 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G143280 PE=4 SV=1)

HSP 1 Score: 349.4 bits (895), Expect = 1.3e-92
Identity = 164/173 (94.80%), Postives = 171/173 (98.84%), Query Frame = 0

Query: 1   MYNGWTVLASWFRLKYPHIALGALASSAPILYFDNITPQDGYYSVVSKSFKEASKTCHDT 60
           +Y+GWTVLASWFRLKYPHIALGALASSAPILYFDNITPQDGYYS+VSKSFKE SKTCHDT
Sbjct: 2   IYHGWTVLASWFRLKYPHIALGALASSAPILYFDNITPQDGYYSIVSKSFKETSKTCHDT 61

Query: 61  IRRSWGEIDRIAEKTQGGLSILSKQFKTCGKLKTSSEIKNLMDTVFTMAAQYNNPYENPV 120
           IRRSWGEIDRIA KT+GGLSILSKQFKTCGKLKTSSEIKNLMD+VFTMAAQYN+PYENPV
Sbjct: 62  IRRSWGEIDRIAGKTRGGLSILSKQFKTCGKLKTSSEIKNLMDSVFTMAAQYNDPYENPV 121

Query: 121 RGICVAIDEEAKKKSNVIKQVVAGLIAYLGERPCYDVYEFGYPNDPLNQYGWQ 174
           RGICVAIDEEAKKKSNVIKQVVAG+IAYLGERPCYDVYEFGYPNDPLNQYGWQ
Sbjct: 122 RGICVAIDEEAKKKSNVIKQVVAGVIAYLGERPCYDVYEFGYPNDPLNQYGWQ 174

BLAST of Chy12G213460 vs. NCBI nr
Match: XP_031739623.1 (lysosomal Pro-X carboxypeptidase [Cucumis sativus] >KAE8649460.1 hypothetical protein Csa_018870 [Cucumis sativus])

HSP 1 Score: 505 bits (1301), Expect = 1.67e-176
Identity = 239/248 (96.37%), Postives = 245/248 (98.79%), Query Frame = 0

Query: 7   VLASWFRLKYPHIALGALASSAPILYFDNITPQDGYYSVVSKSFKEASKTCHDTIRRSWG 66
           +LASWFRLKYPHIALGALASSAPILYFDNITPQDGYYS+VSKSFKE SKTCHDTIRRSWG
Sbjct: 193 MLASWFRLKYPHIALGALASSAPILYFDNITPQDGYYSIVSKSFKETSKTCHDTIRRSWG 252

Query: 67  EIDRIAEKTQGGLSILSKQFKTCGKLKTSSEIKNLMDTVFTMAAQYNNPYENPVRGICVA 126
           EIDRIA KT+GGLSILSKQFKTCGKLKTSSEIKNLMD+VFTMAAQYN+PYENPVRGICVA
Sbjct: 253 EIDRIAGKTRGGLSILSKQFKTCGKLKTSSEIKNLMDSVFTMAAQYNDPYENPVRGICVA 312

Query: 127 IDEEAKKKSNVIKQVVAGLIAYLGERPCYDVYEFGYPNDPLNQYGWQVCSEMVMPIGISG 186
           IDEEAKKKSNVIKQVVAG+IAYLGERPCYDVYEFGYPNDPLNQYGWQVCSEMVMPIG SG
Sbjct: 313 IDEEAKKKSNVIKQVVAGVIAYLGERPCYDVYEFGYPNDPLNQYGWQVCSEMVMPIGSSG 372

Query: 187 RDKNSMFPPSPFQFNDFKTMCKDLYGVTPRPHWITTFYGGQDIKLVLHRFGSNIIFSNGL 246
           RDKNSMFPPSPFQFNDFKTMCKDLYGVTPRPHWITTFYGGQDIKLVLHRFGSNIIFSNGL
Sbjct: 373 RDKNSMFPPSPFQFNDFKTMCKDLYGVTPRPHWITTFYGGQDIKLVLHRFGSNIIFSNGL 432

Query: 247 KDPYSSGG 254
           KDPYSSGG
Sbjct: 433 KDPYSSGG 440

BLAST of Chy12G213460 vs. NCBI nr
Match: XP_031745605.1 (lysosomal Pro-X carboxypeptidase-like [Cucumis sativus] >KGN64893.2 hypothetical protein Csa_022850 [Cucumis sativus])

HSP 1 Score: 505 bits (1300), Expect = 2.37e-176
Identity = 239/248 (96.37%), Postives = 244/248 (98.39%), Query Frame = 0

Query: 7   VLASWFRLKYPHIALGALASSAPILYFDNITPQDGYYSVVSKSFKEASKTCHDTIRRSWG 66
           +LASWFRLKYPHIALGALASSAPILYFDNITPQDGYYS+VSKSFKE SKTCHDTIRRSWG
Sbjct: 193 MLASWFRLKYPHIALGALASSAPILYFDNITPQDGYYSIVSKSFKETSKTCHDTIRRSWG 252

Query: 67  EIDRIAEKTQGGLSILSKQFKTCGKLKTSSEIKNLMDTVFTMAAQYNNPYENPVRGICVA 126
           EIDRIA KTQGGLSILSKQFKTCGKLKTSSEIKNLMD VFTMAAQYN+PYENPVRGICVA
Sbjct: 253 EIDRIAGKTQGGLSILSKQFKTCGKLKTSSEIKNLMDNVFTMAAQYNDPYENPVRGICVA 312

Query: 127 IDEEAKKKSNVIKQVVAGLIAYLGERPCYDVYEFGYPNDPLNQYGWQVCSEMVMPIGISG 186
           IDEEAKKKSNVIKQVVAG+IAYLGERPCYDVYEFGYPNDPLNQYGWQVCSEMVMPIG SG
Sbjct: 313 IDEEAKKKSNVIKQVVAGVIAYLGERPCYDVYEFGYPNDPLNQYGWQVCSEMVMPIGSSG 372

Query: 187 RDKNSMFPPSPFQFNDFKTMCKDLYGVTPRPHWITTFYGGQDIKLVLHRFGSNIIFSNGL 246
           RDKNSMFPPSPF+FNDFKTMCKDLYGVTPRPHWITTFYGGQDIKLVLHRFGSNIIFSNGL
Sbjct: 373 RDKNSMFPPSPFRFNDFKTMCKDLYGVTPRPHWITTFYGGQDIKLVLHRFGSNIIFSNGL 432

Query: 247 KDPYSSGG 254
           KDPYSSGG
Sbjct: 433 KDPYSSGG 440

BLAST of Chy12G213460 vs. NCBI nr
Match: XP_023538113.1 (lysosomal Pro-X carboxypeptidase-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 400 bits (1027), Expect = 4.51e-135
Identity = 185/248 (74.60%), Postives = 215/248 (86.69%), Query Frame = 0

Query: 7   VLASWFRLKYPHIALGALASSAPILYFDNITPQDGYYSVVSKSFKEASKTCHDTIRRSWG 66
           +LASWFRLKYPHIALGALASSAPILYFDNITPQDGYYS+VSKSFKE S+TCH+TIRRSW 
Sbjct: 189 MLASWFRLKYPHIALGALASSAPILYFDNITPQDGYYSIVSKSFKETSQTCHETIRRSWA 248

Query: 67  EIDRIAEKTQGGLSILSKQFKTCGKLKTSSEIKNLMDTVFTMAAQYNNPYENPVRGICVA 126
           E+DRIA     GL ILSK+FKTC KL  S E+K  +D VFT AAQYNNP+E PVRGIC A
Sbjct: 249 EVDRIANNKPEGLMILSKRFKTCEKLNGSDELKYYLDHVFTSAAQYNNPHEKPVRGICAA 308

Query: 127 IDEEAKKKSNVIKQVVAGLIAYLGERPCYDVYEFGYPNDPLNQYGWQVCSEMVMPIGISG 186
           IDE A+K S+VI+QVVAG+IA++GER CYDV+E G+P++P++ + WQ  SE+VMPIG+SG
Sbjct: 309 IDEAARKNSDVIEQVVAGVIAFMGERDCYDVFESGHPDNPIDPFTWQEYSEIVMPIGVSG 368

Query: 187 RDKNSMFPPSPFQFNDFKTMCKDLYGVTPRPHWITTFYGGQDIKLVLHRFGSNIIFSNGL 246
           +DK+SMFP +PF FN FK  CK LYGV+P PHWITTFYGGQD+KL+LHRFGSNIIFSNGL
Sbjct: 369 KDKDSMFPTAPFDFNKFKKDCKALYGVSPNPHWITTFYGGQDLKLILHRFGSNIIFSNGL 428

Query: 247 KDPYSSGG 254
           KDPYSSGG
Sbjct: 429 KDPYSSGG 436

BLAST of Chy12G213460 vs. NCBI nr
Match: XP_023538112.1 (lysosomal Pro-X carboxypeptidase-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 400 bits (1027), Expect = 5.29e-135
Identity = 185/248 (74.60%), Postives = 215/248 (86.69%), Query Frame = 0

Query: 7   VLASWFRLKYPHIALGALASSAPILYFDNITPQDGYYSVVSKSFKEASKTCHDTIRRSWG 66
           +LASWFRLKYPHIALGALASSAPILYFDNITPQDGYYS+VSKSFKE S+TCH+TIRRSW 
Sbjct: 189 MLASWFRLKYPHIALGALASSAPILYFDNITPQDGYYSIVSKSFKETSQTCHETIRRSWA 248

Query: 67  EIDRIAEKTQGGLSILSKQFKTCGKLKTSSEIKNLMDTVFTMAAQYNNPYENPVRGICVA 126
           E+DRIA     GL ILSK+FKTC KL  S E+K  +D VFT AAQYNNP+E PVRGIC A
Sbjct: 249 EVDRIANNKPEGLMILSKRFKTCEKLNGSDELKYYLDHVFTSAAQYNNPHEKPVRGICAA 308

Query: 127 IDEEAKKKSNVIKQVVAGLIAYLGERPCYDVYEFGYPNDPLNQYGWQVCSEMVMPIGISG 186
           IDE A+K S+VI+QVVAG+IA++GER CYDV+E G+P++P++ + WQ  SE+VMPIG+SG
Sbjct: 309 IDEAARKNSDVIEQVVAGVIAFMGERDCYDVFESGHPDNPIDPFTWQEYSEIVMPIGVSG 368

Query: 187 RDKNSMFPPSPFQFNDFKTMCKDLYGVTPRPHWITTFYGGQDIKLVLHRFGSNIIFSNGL 246
           +DK+SMFP +PF FN FK  CK LYGV+P PHWITTFYGGQD+KL+LHRFGSNIIFSNGL
Sbjct: 369 KDKDSMFPTAPFDFNKFKKDCKALYGVSPNPHWITTFYGGQDLKLILHRFGSNIIFSNGL 428

Query: 247 KDPYSSGG 254
           KDPYSSGG
Sbjct: 429 KDPYSSGG 436

BLAST of Chy12G213460 vs. NCBI nr
Match: KAG6586010.1 (Lysosomal Pro-X carboxypeptidase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 398 bits (1022), Expect = 3.02e-134
Identity = 184/248 (74.19%), Postives = 216/248 (87.10%), Query Frame = 0

Query: 7   VLASWFRLKYPHIALGALASSAPILYFDNITPQDGYYSVVSKSFKEASKTCHDTIRRSWG 66
           +LASWFRLKYPHIALGALASSAPILYFDNITPQDGYYS+VSKSFKE S+TC++TIRRSW 
Sbjct: 189 MLASWFRLKYPHIALGALASSAPILYFDNITPQDGYYSIVSKSFKETSQTCYETIRRSWA 248

Query: 67  EIDRIAEKTQGGLSILSKQFKTCGKLKTSSEIKNLMDTVFTMAAQYNNPYENPVRGICVA 126
           EIDRIA+    GL ILSK+FKTC KL  S E+KN +D V T AAQYNNP+E PVRGIC A
Sbjct: 249 EIDRIAKNKPEGLMILSKRFKTCEKLNGSHELKNHLDKVLTSAAQYNNPHEKPVRGICAA 308

Query: 127 IDEEAKKKSNVIKQVVAGLIAYLGERPCYDVYEFGYPNDPLNQYGWQVCSEMVMPIGISG 186
           IDE A+K S+VI+QVVAG++A++GER CYDV+E G+P++P++ + WQ  SE+VMPIG+SG
Sbjct: 309 IDEAARKNSDVIEQVVAGVVAFMGERDCYDVFESGHPDNPIDPFTWQEYSEIVMPIGVSG 368

Query: 187 RDKNSMFPPSPFQFNDFKTMCKDLYGVTPRPHWITTFYGGQDIKLVLHRFGSNIIFSNGL 246
           +DK+SMFP +PF FN FK  CK LYGV+P PHWITTFYGGQD+KL+LHRFGSNIIFSNGL
Sbjct: 369 KDKDSMFPTAPFDFNKFKKDCKALYGVSPNPHWITTFYGGQDLKLILHRFGSNIIFSNGL 428

Query: 247 KDPYSSGG 254
           KDPYSSGG
Sbjct: 429 KDPYSSGG 436

BLAST of Chy12G213460 vs. TAIR 10
Match: AT5G22860.1 (Serine carboxypeptidase S28 family protein )

HSP 1 Score: 280.8 bits (717), Expect = 1.1e-75
Identity = 134/249 (53.82%), Postives = 177/249 (71.08%), Query Frame = 0

Query: 7   VLASWFRLKYPHIALGALASSAPILYFDNITPQDGYYSVVSKSFKEASKTCHDTIRRSWG 66
           +LA+WFRLKYPHIALGALASSAP+LYF++  P+ GYY +V+K FKEAS+ C++TIR SW 
Sbjct: 194 MLAAWFRLKYPHIALGALASSAPLLYFEDTRPKFGYYYIVTKVFKEASERCYNTIRNSWI 253

Query: 67  EIDRIAEKTQGGLSILSKQFKTCGKLKTSSEIKNLMDTVFTMAAQYNNPYENPVRGICVA 126
           EIDR+A K   GLSILSKQFKTC  L  S +IK+ +DT++  A QYN      V  +C A
Sbjct: 254 EIDRVAGK-PNGLSILSKQFKTCAPLNGSFDIKDFLDTIYAEAVQYNRGPNFWVAKVCNA 313

Query: 127 ID-EEAKKKSNVIKQVVAGLIAYLGERPCYDVYEFGYPNDPLNQYGWQVCSEMVMPIGIS 186
           I+     ++ N++ ++ AG++A +G R CYD   F  P +    + WQ CSE+VMP+G  
Sbjct: 314 INANPPNRRYNLLDRIFAGVVALVGNRTCYDTKMFAQPTNNNIAWRWQSCSEIVMPVGYD 373

Query: 187 GRDKNSMFPPSPFQFNDFKTMCKDLYGVTPRPHWITTFYGGQDIKLVLHRFGSNIIFSNG 246
            +D  +MFP +PF    +   CK  +GVTPRPHWITT++G Q++KL+L +FGSNIIFSNG
Sbjct: 374 KQD--TMFPTAPFNMTSYIDGCKSYHGVTPRPHWITTYFGIQEVKLILQKFGSNIIFSNG 433

Query: 247 LKDPYSSGG 255
           L DPYS GG
Sbjct: 434 LSDPYSVGG 439

BLAST of Chy12G213460 vs. TAIR 10
Match: AT5G22860.2 (Serine carboxypeptidase S28 family protein )

HSP 1 Score: 280.8 bits (717), Expect = 1.1e-75
Identity = 134/249 (53.82%), Postives = 177/249 (71.08%), Query Frame = 0

Query: 7   VLASWFRLKYPHIALGALASSAPILYFDNITPQDGYYSVVSKSFKEASKTCHDTIRRSWG 66
           +LA+WFRLKYPHIALGALASSAP+LYF++  P+ GYY +V+K FKEAS+ C++TIR SW 
Sbjct: 194 MLAAWFRLKYPHIALGALASSAPLLYFEDTRPKFGYYYIVTKVFKEASERCYNTIRNSWI 253

Query: 67  EIDRIAEKTQGGLSILSKQFKTCGKLKTSSEIKNLMDTVFTMAAQYNNPYENPVRGICVA 126
           EIDR+A K   GLSILSKQFKTC  L  S +IK+ +DT++  A QYN      V  +C A
Sbjct: 254 EIDRVAGK-PNGLSILSKQFKTCAPLNGSFDIKDFLDTIYAEAVQYNRGPNFWVAKVCNA 313

Query: 127 ID-EEAKKKSNVIKQVVAGLIAYLGERPCYDVYEFGYPNDPLNQYGWQVCSEMVMPIGIS 186
           I+     ++ N++ ++ AG++A +G R CYD   F  P +    + WQ CSE+VMP+G  
Sbjct: 314 INANPPNRRYNLLDRIFAGVVALVGNRTCYDTKMFAQPTNNNIAWRWQSCSEIVMPVGYD 373

Query: 187 GRDKNSMFPPSPFQFNDFKTMCKDLYGVTPRPHWITTFYGGQDIKLVLHRFGSNIIFSNG 246
            +D  +MFP +PF    +   CK  +GVTPRPHWITT++G Q++KL+L +FGSNIIFSNG
Sbjct: 374 KQD--TMFPTAPFNMTSYIDGCKSYHGVTPRPHWITTYFGIQEVKLILQKFGSNIIFSNG 433

Query: 247 LKDPYSSGG 255
           L DPYS GG
Sbjct: 434 LSDPYSVGG 439

BLAST of Chy12G213460 vs. TAIR 10
Match: AT2G24280.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 206.8 bits (525), Expect = 2.0e-53
Identity = 110/259 (42.47%), Postives = 151/259 (58.30%), Query Frame = 0

Query: 7   VLASWFRLKYPHIALGALASSAPILYFDNITPQDGYYSVVSKSFKEASKTCHDTIRRSWG 66
           +LA+WFRLKYPHI +GALASSAPIL+FDNI P   +Y  +S+ FK+AS  C   I+RSW 
Sbjct: 181 MLAAWFRLKYPHITIGALASSAPILHFDNIVPLTSFYDAISQDFKDASINCFKVIKRSWE 240

Query: 67  EIDRIAEKTQGGLSILSKQFKTCGKLKTSSEIKNLMDTVFTMAAQYNNPYE--------- 126
           E++ ++   + GL  LSK+F+TC  L +    ++ +   F   A  N P           
Sbjct: 241 ELEAVS-TMKNGLQELSKKFRTCKGLHSQYSARDWLSGAFVYTAMVNYPTAANFMAPLPG 300

Query: 127 NPVRGICVAIDEEAKKKSNVIKQVVAGLI--AYLGERPCYDVYEFGYPNDPLNQYGWQVC 186
            PV  +C  ID   +  SN+ +   A  +   Y G   C+++ E    +  L+ + +Q C
Sbjct: 301 YPVEQMCKIIDGFPRGSSNLDRAFAAASLYYNYSGSEKCFEM-EQQTDDHGLDGWQYQAC 360

Query: 187 SEMVMPIGISGRDKNSMFPPSPFQFNDFKTMCKDLYGVTPRPHWITTFYGGQDIKLVLHR 246
           +EMVMP+  S +   SM PP       F+  C   YGV PRPHWITT +GG  I+ VL R
Sbjct: 361 TEMVMPMSCSNQ---SMLPPYENDSEAFQEQCMTRYGVKPRPHWITTEFGGMRIETVLKR 420

Query: 247 FGSNIIFSNGLKDPYSSGG 255
           FGSNIIFSNG++DP+S GG
Sbjct: 421 FGSNIIFSNGMQDPWSRGG 434

BLAST of Chy12G213460 vs. TAIR 10
Match: AT5G65760.1 (Serine carboxypeptidase S28 family protein )

HSP 1 Score: 203.4 bits (516), Expect = 2.2e-52
Identity = 103/257 (40.08%), Postives = 153/257 (59.53%), Query Frame = 0

Query: 7   VLASWFRLKYPHIALGALASSAPILYFDNITPQDGYYSVVSKSFKEASKTCHDTIRRSWG 66
           +LA+W RLKYPHIA+GALASSAPIL F+++ P + +Y + S  FK  S +C +TI+ SW 
Sbjct: 196 MLAAWMRLKYPHIAIGALASSAPILQFEDVVPPETFYDIASNDFKRESSSCFNTIKDSWD 255

Query: 67  EIDRIAE-KTQGGLSILSKQFKTCGKLKTSSEIKNLMDTVFTMAAQYNNPYE-------- 126
            I  IAE + + GL  L+K F  C  L ++ ++ + +D+ ++  A  + PY         
Sbjct: 256 AI--IAEGQKENGLLQLTKTFHFCRVLNSTDDLSDWLDSAYSYLAMVDYPYPADFMMPLP 315

Query: 127 -NPVRGICVAIDEEAKKKSNVIKQVVAGLIAYLGERPCYDVYEFGYPNDPLNQYGWQVCS 186
            +P+R +C  ID  A   ++++ ++ AG+  Y       D ++       L+ + WQ C+
Sbjct: 316 GHPIREVCRKID-GAGSNASILDRIYAGISVYYNYTGNVDCFKLDDDPHGLDGWNWQACT 375

Query: 187 EMVMPIGISGRDKNSMFPPSPFQFNDFKTMCKDLYGVTPRPHWITTFYGGQDIKLVLHRF 246
           EMVMP  +S   +NSMFP   F ++ +K  C + + V PRP W+TT +GG DI   L  F
Sbjct: 376 EMVMP--MSSNQENSMFPGYGFNYSSYKEECWNTFRVNPRPKWVTTEFGGHDIATTLKSF 435

Query: 247 GSNIIFSNGLKDPYSSG 254
           GSNIIFSNGL DP+S G
Sbjct: 436 GSNIIFSNGLLDPWSGG 447

BLAST of Chy12G213460 vs. TAIR 10
Match: AT3G28680.1 (Serine carboxypeptidase S28 family protein )

HSP 1 Score: 146.0 bits (367), Expect = 4.2e-35
Identity = 79/170 (46.47%), Postives = 109/170 (64.12%), Query Frame = 0

Query: 7   VLASWFRLKYPHIALGALASSAPILYFDNITPQDGYYSVVSKSFKEASKTCHDTIRRSWG 66
           VLA+WF+LKYP+IALGALASSAP+LYF++  P+ GY+ +V+K FKE SK CH+ I +SW 
Sbjct: 23  VLAAWFKLKYPYIALGALASSAPLLYFEDTLPKHGYFYIVTKVFKEMSKECHNKIHKSWD 82

Query: 67  EIDRIAEKTQGGLSILSKQFKTCGKLKTSSEIKNLMDTVFTMAAQYNNPYENPVRGICVA 126
           EIDRIA K    LSILSK FK C  L    E+K+ +  ++   AQY++   +  R +C A
Sbjct: 83  EIDRIAAK-PNSLSILSKNFKLCNPLNDIIELKSYVSYIYARTAQYSDNQFSVAR-LCEA 142

Query: 127 ID-EEAKKKSNVIKQVVAGLIAYLGERPCYDVYEFGY--PNDPLNQYGWQ 174
           I+      KS+++ Q+ AG++A  G   CY +    Y   ND    +GWQ
Sbjct: 143 INTSPPNTKSDLLDQIFAGVVASRGNISCYGMSSPSYQMTNDD-RAWGWQ 189

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q2TA141.4e-3834.98Lysosomal Pro-X carboxypeptidase OS=Bos taurus OX=9913 GN=PRCP PE=2 SV=1[more]
Q5RBU73.7e-3633.96Lysosomal Pro-X carboxypeptidase OS=Pongo abelii OX=9601 GN=PRCP PE=2 SV=1[more]
P427851.4e-3533.58Lysosomal Pro-X carboxypeptidase OS=Homo sapiens OX=9606 GN=PRCP PE=1 SV=1[more]
Q7TMR01.4e-3533.96Lysosomal Pro-X carboxypeptidase OS=Mus musculus OX=10090 GN=Prcp PE=1 SV=2[more]
Q9EPB14.1e-2730.37Dipeptidyl peptidase 2 OS=Rattus norvegicus OX=10116 GN=Dpp7 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1FJ874.9e-10873.79lysosomal Pro-X carboxypeptidase-like OS=Cucurbita moschata OX=3662 GN=LOC111444... [more]
A0A6J1FDJ07.9e-10674.19lysosomal Pro-X carboxypeptidase-like OS=Cucurbita moschata OX=3662 GN=LOC111444... [more]
A0A6J1DJ731.9e-9972.69lysosomal Pro-X carboxypeptidase-like OS=Momordica charantia OX=3673 GN=LOC11102... [more]
A0A6J1DHH27.1e-9971.08lysosomal Pro-X carboxypeptidase-like OS=Momordica charantia OX=3673 GN=LOC11102... [more]
A0A0A0LV801.3e-9294.80Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G143280 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_031739623.11.67e-17696.37lysosomal Pro-X carboxypeptidase [Cucumis sativus] >KAE8649460.1 hypothetical pr... [more]
XP_031745605.12.37e-17696.37lysosomal Pro-X carboxypeptidase-like [Cucumis sativus] >KGN64893.2 hypothetical... [more]
XP_023538113.14.51e-13574.60lysosomal Pro-X carboxypeptidase-like isoform X2 [Cucurbita pepo subsp. pepo][more]
XP_023538112.15.29e-13574.60lysosomal Pro-X carboxypeptidase-like isoform X1 [Cucurbita pepo subsp. pepo][more]
KAG6586010.13.02e-13474.19Lysosomal Pro-X carboxypeptidase, partial [Cucurbita argyrosperma subsp. sororia... [more]
Match NameE-valueIdentityDescription
AT5G22860.11.1e-7553.82Serine carboxypeptidase S28 family protein [more]
AT5G22860.21.1e-7553.82Serine carboxypeptidase S28 family protein [more]
AT2G24280.12.0e-5342.47alpha/beta-Hydrolases superfamily protein [more]
AT5G65760.12.2e-5240.08Serine carboxypeptidase S28 family protein [more]
AT3G28680.14.2e-3546.47Serine carboxypeptidase S28 family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR042269Serine carboxypeptidase S28, SKS domainGENE3D1.20.120.980Serine carboxypeptidase S28, SKS domaincoord: 38..197
e-value: 2.3E-56
score: 193.8
IPR008758Peptidase S28PFAMPF05577Peptidase_S28coord: 7..254
e-value: 1.3E-27
score: 96.7
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 7..254
e-value: 2.3E-56
score: 193.8
NoneNo IPR availablePANTHERPTHR11010PROTEASE S28 PRO-X CARBOXYPEPTIDASE-RELATEDcoord: 7..254
NoneNo IPR availablePANTHERPTHR11010:SF92LYSOSOMAL PRO-X CARBOXYPEPTIDASE-LIKE ISOFORM X1coord: 7..254

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy12G213460.1Chy12G213460.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0004180 carboxypeptidase activity
molecular_function GO:0008236 serine-type peptidase activity